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First Report of the Isolation of Nocardia thailandica from the Bronchoalveolar Lavage of a Patient in Iran.

Introduction

Nocardiae are Gram-positive, aerobic, slow-growing, and relatively acid-fast bacteria which live as saprophytes in environmental sources such as water, soil, dust, animal excreta, and decaying plants. This group of bacteria can enter the human body through inhalation and skin injuries, creating nocardiosis infection. (1) Nocardia infections can be hosts for immune system disorders, and they can even involve healthy individuals. (2)

More than 30 different species of Nocardia have so far been isolated from clinical samples, among which Nocardia thailandica (N. thailandica) was first introduced by Kageyama and colleagues from the purulent secretions of a Thai patient's abscess. According to the existing reports, N. thailandica is isolated from respiratory and eye infections. (1-3)

Given the scarcity of reports in the existing literature on the isolation of N. thailandica, we sought to conduct the present case study on respiratory infections caused by N. thailandica.

Case Presentation

A 53-year-old Iranian man with chronic bronchitis was admitted to Al-Zahra Hospital, Isfahan, because of mild fever, weight loss, nonproductive cough, anorexia, and chronic chest pain. Diffuse nodular infiltrates and enlargement of blood vessels were observed in the radiography images of the patient's chest (figure 1). There was no apparent evidence of immunodeficiency or HIV infection. Microscopic examination (acid-fast staining) of 3 sputum samples did not reveal acid-fast bacteria. Additionally, the tuberculin test on the patient proved negative. Because the patient's fever and chest pain remained unchanged, the treating physician requested a bronchoalveolar lavage sample. The sample was incubated at 37 [degrees]C in a blood agar medium containing 5% sheep blood, chocolate agar, and brain heart infusion (BHI) agar. White and dry colonies emitting soil odor were observed in the chocolate and BHI mediums after 3 days. A Nocardia-like organism was detected in the direct microscopic evaluation of the patient's bronchoalveolar lavage (Gram-positive and weakly acid-fast with branching filamentous rods) (figure 2). This isolate was subjected to identification by resistance to lysozyme broth and conventional phenotypic tests. (1)

Primarily, the bacterial genome was extracted via the boiling method for molecular investigations. Then, 27-F (5'-AGAGTTTGATCCTGGCTCAG-3') and 1492-R (5'-GGTTACCTTGTTACGACTT-3') primers were used to reproduce the 16S rRNA gene as recommended by Morelli et al. (4) Sequencing was performed by Bioneer Corporation (South Korea), and the sequence data received were aligned using CLC Main Workbench 5 software with the existing sequences of Nocardia retrieved from the GenBank in the National Center for Biotechnology Information (NCBI) website database. The resultant data were thereafter analyzed using the Basic Local Alignment Search Tool (BLAST) program in GenBank. According to the results, the considered microorganism was identified as N. thailandica and the studied isolation sequences were registered in the NCBI under accession No. KC577173.1. The partial 16S rRNA gene sequences (1387 bp) of the isolate revealed 99% similarities with those of the N. thailandica strain DSM 44808.

The disk diffusion method was utilized to test the determination of the antimicrobial susceptibility test for the considered isolation. The considered microorganism was sensitive to trimethoprim-sulfamethoxazole, amikacin, and linezolid, while it was reported to be resistant to penicillin-G and ciprofloxacin. The patient was treated with trimethoprim-sulfamethoxazole (480 mg) and linezolid (600 mg) for 6 months and his symptoms eventually disappeared completely. Informed consent to report this case was obtained from the patient.

Discussion

Nocardia spp. are Gram-positive, weakly acid-fast, nonspore-forming, filamentous, branching, obligatory aerobic, and relatively slow-growing bacteria which often form branched hyphae in both tissue and culture. The hyphae are easily disrupted into rods and cocci elements. Nocardia spp. are found extensively around the world as a saprophytic component of the soil, water, dust, and air. Nocardia was first isolated by Edmond Nocard in 1888 from a case of bovine farcy. A year after, an Italian researcher, Trevisan, characterized the organism and named it N. farcinica. (1)

The prevalence of Nocardia infections has risen in recent years. This phenomenon can be the result of factors such as increasing numbers of patients with immunodeficiency and the use of advanced molecular methods. (5,1) The detection of clinically significant Nocardia spp. is important for the definitive diagnosis, prediction of antimicrobial susceptibility, epidemiological purposes, and effective treatment. (1,3) Owing to the similarity between Nocardiae and fungi and the non-exclusivity of clinical manifestations as well as radiologic findings in nocardiosis infections, Nocardia infections can be mistaken with fungal infections and mycobacteria. (5,6) Molecular methods can identify Nocardia infections with acceptable precision and exclusivity. For instance, finding the sequence of the 16S rRNA gene is one of the best alternatives for determining and comparing Nocardia spp. (7,8) Corticosteroid and immunosuppression treatment of a patient with chronic bronchitis is one of the most important risk factors in respiratory nocardiosis infections. (9)

Shahraki et al. (8) collected 127 clinical isolates of Nocardia spp. from patients living in 5 difference Iranian provinces and found that N. cyriacigeorgica and N. asteroids were the most common species isolated from Iranian patients with nocardiosis. They also reported that trimethoprim-sulfamethoxazole and linezolid were respectively the first- and second-line antibiotics against nocardiosis. The most common manifestation of Nocardia infections is the pulmonary form, and the first isolate of N. cyriacigeorgica was from a patient affected by chronic bronchitis. (6) Canterino et al. (10) (2015) published a case study on N. thailandica respiratory infection in a patient receiving a transplantation. Whereas the patient in their study was immunocompromised, we isolated this species from an older man. Thus, N. thailandica is an opportunistic pathogen that has emerged in recent years. Reddy et al. (11) reported a single case of keratitis caused by N. thailandica. There is a dearth of data on the isolation of N. thailandica. In the present study, the microorganism isolated from the patient suffering from chronic bronchitis was evaluated via phenotypic and molecular tests and identified as N. thailandica. Based on DNA sequences, N. thailandica is closely related to N. asteroides, N. neocaledoniensis, and N. caishijiensis. Therefore, the identification of this species requires trained technicians. (10) In Iran, Shahraki et al. (6) isolated 11 different Nocardia spp. from 46 patients with nocardiosis and reported that N. asteroides and N. veterana were respectively the most and least frequent Nocardia spp. isolated from the patients. Nocardia identification at the species level is vitally important for appropriate treatment, production of secondary metabolisms especially antibiotics, and epidemiologic and geographic studies. (12)

Our literature review yielded precious few clinical reports about the isolation of N. thailandica around the globe. Because this species is susceptible to the majority of antimicrobials for other Nocardia spp., (8,10) the isolation and identification of this species is important for epidemiological studies into the distribution of Nocardia spp. in the world. N. thailandica IFM [10145.sup.T] was first isolated as a novel species in 2004 from a wound infection (3) and has also been reported from pulmonary and keratitis infections. N. thailandica infections can be treated with trimethoprim-sulfamethoxazole, imipenem, amikacin, tobramycin, and the third generation of cephalosporins. (3,10)

Our report may help clarify the taxonomy of N. thailandica and its role as a member of the genus Nocardia, which can cause nocardiosis in men, specially pulmonary nocardiosis.

Conclusion

N. thailandica is a possible emerging pathogen within the Nocardia genus and is often accompanied by pulmonary infections. Molecular methods can identify Nocardia spp. faster and better than conventional methods. Due to the similarity between N. thailandica and N. asteroides, 16S rRNA gene sequencing is the best method for the rapid and accurate identification and differentiation of Nocardia spp.

Acknowledgment

We wish to thank Isfahan University of Medical Sciences for supporting this study via grant No. 390510.

Conflict of Interest: None declared.

References

(1.) Brown-Elliott BA, Brown JM, Conville PS, Wallace RJ, Jr. Clinical and laboratory features of the Nocardia spp. based on current molecular taxonomy. Clin Microbiol Rev. 2006;19:259-82. doi: 10.1128/CMR.19.2.259-282.2006. PubMed PMID: 16614249; PubMed Central PMCID: PMCPMC1471991.

(2.) Simpson GL, Stinson EB, Egger MJ, Remington JS. Nocardial infections in the immunocompromised host: A detailed study in a defined population. Rev Infect Dis. 1981;3:492-507. PubMed PMID: 7025149.

(3.) Kageyama A, Poonwan N, Yazawa K, Suzuki S-i, Kroppenstedt RM, Mikami Y. Nocardia vermiculata sp. nov. and Nocardia thailandica sp. nov. isolated from clinical specimens. Actinomycetologica. 2004;18:27-33. doi: 10.3209/saj.18_27.

(4.) Madueno L, Coppotelli B, Alvarez H, Morelli I. Isolation and characterization of indigenous soil bacteria for bioaugmentation of PAH contaminated soil of semiarid Patagonia, Argentina. Int Biodeterior Biodegradation. 2011;65:345-51. doi: 10.1016/j.ibiod.2010.12.008.

(5.) Hau SC, Dart JK, Vesaluoma M, Parmar DN, Claerhout I, Bibi K, et al. Diagnostic accuracy of microbial keratitis with in vivo scanning laser confocal microscopy. Br J Ophthalmol. 2010;94:982-7. doi: 10.1136/bjo.2009.175083. PubMed PMID: 20538659.

(6.) Hashemi-Shahraki A, Bostanabad SZ, Heidarieh P, Sheikhi N, Biranvand M, Alavi SM, et al. Species spectrum of Nocardia spp. isolated from suspected tuberculosis patients. Health. 2015;7:847-58. doi: 10.4236/health.2015.77100.

(7.) Conville PS, Witebsky FG. Multiple copies of the 16S rRNA gene in Nocardia nova isolates and implications for sequence-based identification procedures. J Clin Microbiol. 2005;43:2881-5. doi: 10.1128/JCM.43.6.2881-2885.2005. PubMed PMID: 15956412; PubMed Central PMCID: PMCPMC1151890.

(8.) Hashemi-Shahraki A, Heidarieh P, Bostanabad SZ, Hashemzadeh M, Feizabadi MM, Schraufnagel D, et al. Genetic diversity and antimicrobial susceptibility of Nocardia species among patients with nocardiosis. Sci Rep. 2015;5:17862. doi: 10.1038/srep17862. PubMed PMID: 26638771; PubMed Central PMCID: PMCPMC4671095.

(9.) Angrill J, Agusti C, de Celis R, Rano A, Gonzalez J, Sole T, et al. Bacterial colonisation in patients with bronchiectasis: microbiological pattern and risk factors. Thorax. 2002;57:15-9. doi: 10.1136/thorax.57.1.15. PubMed PMID: 11809984; PubMed Central PMCID: PMCPMC1746176.

(10.) Canterino J, Paniz-Mondolfi A, Brown-Elliott BA, Vientos W, Vasireddy R, Wallace RJ, Jr., et al. Nocardia thailandica Pulmonary Nocardiosis in a Post-Solid Organ Transplant Patient. J Clin Microbiol. 2015;53:3686-90. doi: 10.1128/JCM.00959-15. PubMed PMID: 26292312; PubMed Central PMCID: PMCPMC4609689.

(11.) Reddy AK, Garg P, Kaur I. Spectrum and clinicomicrobiological profile of Nocardia keratitis caused by rare species of Nocardia identified by 16S rRNA gene sequencing. Eye (Lond). 2010;24:1259-62. doi: 10.1038/eye.2009.299. PubMed PMID: 19960036.

(12.) Bafghi MF, Heidarieh P, Habibnia S, Rasouli-Nasab M, Kalantar Neyestanaki D, Afshar D, et al. Phenotypic and molecular properties of the Nocardia species. Avecinna J Clin Microb Infect. 2014;1:e19215. doi: 10.17795/ajcmi-19215.

Samaneh Bourbour (1), PhD;

Masoud Keikha (2), MSc;

Jamshid Faghri (2), PhD

(1) Department of Microbiology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran;

(2) Department of Microbiology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran

Correspondence:

Jamshid Faghri, MD;

Department of Microbiology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran

Tel: +98 3137929021

Fax: +98 9131148618

Email: faghri@med.mui.ac.ir

Received: 11 July 2017

Revised: 5 August 2017

Accepted: 20 August 2017

What's Known

* Nocardia thailandica is an emerging pathogen.

What's New

* This manuscript is the first report of the isolation of Nocardia thailandica from Iran.

* Isolation of this microorganism is important in epidemiological studies.
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Title Annotation:Case Report
Author:Bourbour, Samaneh; Keikha, Masoud; Faghri, Jamshid
Publication:Iranian Journal of Medical Sciences
Date:Sep 1, 2018
Words:1856
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