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Factors Controlling the Expansion Behavior of Favia favus (Cnidaria: Scleractinia): Effects of Light, Flow, and Planktonic Prey.

O. LEVY [*]

Abstract. Colonies of the massive stony coral Favia favus were exposed to different flow speeds and levels of light, and to the addition of zooplankton prey. The relative importance of each factor in controlling polyp expansion behavior was tested. The coral polyps fully expanded when they were exposed to low light intensity (0-40 [micro]mol [m.sup.-2] [s.sup.-1] and high flow speed (15 cm [s.sup.-1]), regardless of prey presence. They also partially expanded under low and medium light (40-80 [micro]mol [m.sup.-2] [s.sup.-1]) at medium flow speed (10 cm [s.sup.-1]. The corals expanded their polyps only when they were exposed to light levels below compensation irradiance ([I.sub.com]: light level at which photosynthesis = respiration), which was determined to be about 107 [+ or -] 24 [micro]mol [m.sup.-2] [s.sup.-1].

The results presented here indicate that high flow, low light, and the presence of planktonic prey induce coral expansion. There is a hierarchy of response to these stimuli, in which light level and flow speed are dominant over prey presence. Coral response to these three factors is probably due to the relative importance of gas exchange and zooplankton prey.

Introduction

Diel patterns of polyp expansion and contraction vary between species of anthozoans, including reef-building corals (Kawaguti, 1954; Porter, 1974; Lasker, 1979), gorgonians (Wainwright, 1967), and sea anemones (Pearse, 1974; Sebens and DeRiemer, 1977). Most reef corals expand their tentacles nocturnally, but some expand continuously or only during the day (Abe, 1939; Porter, 1974; Lasker, 1979).

Corals that expand tentacles at night remain open until dawn, but a beam of light or mechanical stimulation may cause them to contract immediately (Abe, 1939). Sea anemones show a similar range of behavior. Structures containing high densities of zooxanthellae (such as pseudotentacles and column vesicles) may expand under conditions favorable for photosynthesis, whereas structures that contain few or no zooxanthellae (such as feeding tentacles) contract under these conditions (Sebens and DeRiemer, 1977). In the stony coral Plerogyra sinuosa, vesicles with high zooxanthellae density expand only during daytime, and feeding tentacles expand at night (Vareschi and Fricke, 1986). Likewise, polyps of the encrusting gorgonian Erythropodium caribbaeorum have pinnate tentacles with dense populations of zooxanthellae that do not function in nocturnal feeding and are expanded during the day (Sebens and Miles, 1988). It is assumed that, since such structures contract when their main function is impeded, their expansion i nvolves some form of energy cost. When feeding tentacles themselves contain dense populations of zooxanthellae, they serve both functions of prey capture and photosynthesis, and generally are expanded continuously.

Zooplankton prey are most abundant at night on coral reefs (Sorokin, 1990), so it has been assumed that most corals expand their tentacles nocturnally in order to capture prey (Porter, 1974; Lewis and Price, 1975). Tentacular expansion also is affected by water flow, which reduces the thickness of the boundary layer over tissues and enhances gas exchange (Shashar et al., 1993). Increases in water flow result in higher (approximately doubled) rates of respiration by sea anemones, stony corals, and soft corals (Patterson and Sebens, 1989; Patterson et al., 1991).

The effect of flow on respiration is likely to be magnified when the exposed tissue surface area is increased by extension of either tentacles or specialized column structures. Low flow rate limits the diffusion of materials in the water column to and from coral tissues, thereby regulating and reducing metabolic rates (Patterson and Sebens, 1989; Patterson et al., 1991), zooxanthellae productivity (Dennison and Barnes, 1988; Shick, 1990; Patterson et al., 1991), and nutrient uptake (Atkinson and Bilger, 1992). Oxygen builds up to high levels within and above coral tissue surfaces during the day as a result of photosynthesis, and is extremely depleted near those surfaces at night due to respiration; both gradients are reduced, improving delivery of dissolved substances to and from tissues, with even small increases of flow speed (Shashar and Stambler, 1992; Kuhl et al,, 1995; Shashar et al., 1996).

Polyp size, tentacle morphology, and tissue surface area vary widely among coral species, and all three factors contribute to prey encounter and capture rate. Porter (1976) suggested that large polyps with elongate tentacles specialize in zooplankton capture, at the expense of high surface area conducive to photosynthesis. However, more recent evidence shows that certain small-polyped corals are able to capture almost the same size range of prey as do those with larger polyps, and actually have much higher rates of prey capture per unit biomass because of the high surface area of the tentacles that they expose to moving water (Sebens, 1987a). The small-polyped coral Madracis mirabilis captures 30 times more zooplankton per coral biomass than does the much larger-polyped Montastrea cavernosa (Sebens et al., 1996). The biomass of polyps of M. mirabilis is only 1/90th of the biomass of M. cavernosa polyps, but an equal biomass of M. the colony has 12 times the tentacular surface area of a M. cavernosa colony.

A primary cost associated with expansion in corals is increased respiration, facilitated by a larger surface area of tissue in contact with the overlying water, including water inside the coelenteron, than in contracted corals. Polyp expansion is energy consuming, in that it is achieved by pressurization of the coelenteron. Pressurization is brought about by ciliary pumping of water into the coelenteron, which acts on the viscoelastic material of the polyp wall (Barnes, 1987).

Rates of respiration are known to be significantly reduced in contracted corals (Brafield and Chapman, 1965; Pearse, 1974; Robbins, and Shick, 1980; Lasker, 1981). The magnitude of this difference depends on ambient oxygen concentration (Beattie, 1971; Sassaman and Mangum, 1972, 1973, 1974; Shumway, 1978) and on the ratio of expanded to contracted tissue area, rather than being a simple response to low external oxygen concentration alone. Contraction may be a means of producing a low oxygen concentration in the coelenteron, resulting in lower metabolic rates and in savings of energy or carbon regardless of the external oxygen level (Sebens, 1987b). In contrast, expansion may aid in the diffusion of excess oxygen away from photosyn-thesizing coral tissues, thus reducing local hyperoxia and its negative effect on metabolic rates or damage due to super-oxide radicals (Lesser and Shick, 1989; Shick, 1991). On the other hand, contraction could result in anaerobic metabolism and oxygen debt.

The effects of polyp expansion and contraction on gas exchange and on the key metabolic processes of photosynthesis and respiration are unknown in stony corals. In some cases, photosynthesis contributes all of the required carbon for corals, as in the shallow-water, high-light-adapted Stylophora pistillata (Falkowski et al., 1984), whereas in low-light-adapted S. pistillata (Falkowski et al., 1984) and in other coral species that depend mostly on predation, photosynthesis supplies only a small fraction of energy demand. Since, in the course of photosynthesis, oxygen is evolved while carbon dioxide is consumed, it is possible that in some corals and other zooxanthellate organisms, the extension and contraction of tentacles is tightly coupled to photosynthetic rates. Tentacle expansion status in corals affects the amount of light absorbed by the zooxanthellae, and in turn photosynthetic rates determine [O.sub.2] and [CO.sub.2] concentrations in the coral tissue and surrounding waters.

Predation on coral tentacles may cause their retraction at times when predators are active. However, a considerable number of coral species with zooxanthellate tentacles expand during the daytime when many coral predators (e.g., chaetodontid fish) are active, suggesting that predation on tentacles is not a currently active selective force on these behavioral patterns.

On coral reefs at Eilat (Gulf of Aqaba, northern Red Sea), colonies of the massive stony coral Favia favus expand their tentacles only after sunset and remain open until sunrise (O. Levy, unpub. obs.). In the present study, we determined the relative importance of flow speed, photosynthetically active radiation (PAR), and planktonic prey availability in controlling tentacle expansion behavior in Favia favus.

Materials and Methods

Coral collection and flow tank setup

Colonies of the common coral Favia favus were collected from the reef adjacent to the Interuniversity Institute for Marine Science at Eilat, Israel, northern Red Sea, from depths of 5-7 m. Individuals of this species were chosen for study because they are abundant on coral reefs at Eilat, their polyps are large (about 1 cm diameter) and easy to observe, and they exhibit regular diel behavior under laboratory conditions. The collected coral colonies, each up to 10 cm diameter, were transferred to the laboratory and placed in shallow tanks supplied with running seawater for 10 h of acclimation before experiments were begun. New corals were collected for each combination of experimental treatments.

Experiments on tentacle expansion behavior were performed in two 25-1 recirculating flow tanks; procedures were based on those described by Vogel and

LaBarbera (1978). Each tank was 100 cm long X 10 cm wide X 25 cm high, and seawater was circulated with a 12-V DC motor. Electronic function generators, consisting of computer programs that were interfaced through a digital-to-analog circuit, controlled the motor speed. Flow speed was measured by following the movement of brightly illuminated particles that were added to the water. Particle movements were recorded using a video camera (Sony CCD 2000E, Hi8 PAL system) with a close-up lens. Flow velocity was measured as described by Trager et al. (1990), using a videocassette recorder (Sony EVO-9800P) for frame-to-frame tracking of the movement of the back-lit particles suspended in the flowing water.

Coral expansion experiments

Colonies of F. favus were tested at three flow speeds: 5, 10, and 15 cm [s.sup.-1] These speeds were selected as similar to natural flow speeds on coral reefs at Eilat, which range between about 5 and 20 cm [s.sup.-1] (Genin et al., 1994). For each set of experiments, 20 colonies were used; five corals were placed in each of four tanks. Two of the tanks served as controls with no flow, while in the other two tanks flow was generated as described above. Halogen lamps coupled to fiber optics were set above all four tanks within each experiment, and three light irradiance levels were tested-- 40, 80, and 120 [micro]mol [m.sup-2] [s.sup.-1] (equivalent to 40, 80, and 120 [micro]E [m.sup.-2][s.sup.-1]) Irradiance was measured as photon flux, with a QSI-140 quantum scalar irradiance meter. Temperature was maintained at 24 [+ or -] 0.1[degrees]C. Each experiment lasted 180 mm, and coral expansion behavior was recorded every 10 min. The tanks were rinsed between experiments. In feeding experiments, freshly hatched Artemia nauplii were added ad libidum. Tentacle expansion of F. favus was scored on a scale based on Lasker (1979), ranging from 0 to 4, where 0 represents 0% expansion (full contraction), 1 is 25% expansion, 2 is 50% expansion, 3 is 75% expansion, and 4 is 100% expansion (Fig. 1). Each score refers to a whole coral colony (Fig. 1).

The first set of three experiments was conducted at a flow speed of 5 cm [s.sup.-1], with three levels of irradiance (40, 80, 120 [micro]mol [m.sup.-2][s.sup.-1])). In one of the two tanks with water flow, and in one of the control tanks without flow, Artemia nauplii serving as zooplankton prey were added after 90 min. In the second set of three experiments, the flow speed was set to 10 cm[s.sup.-1])and the last set of three experiments was run with new colonies of F. favus at a flow speed of 15 cm [s.sup.-1]. Thus, in the three sets of experiments, corals were exposed to all possible combinations of water flow, light intensity, and prey presence. The corals were replaced with new colonies in each experiment to avoid changes in behavior with experience. At the beginning of all experiments, the tentacles of the F. favus colonies were contracted (Fig. 1).

We assessed possible interaction effects between light intensity, prey presence, and flow speed on tentacle expansion behavior in F. favus by performing three-way ANOVA tests on the results.

Determination of light compensation point ([I.sub.com])

The light compensation point for F. favus was determined by measuring coral respiration in a closed system (Haramaty et al., 1997). Photosynthesis (under different light intensities) and dark respiration were measured as changes in oxygen concentration, using a Clark-type [O.sub.2] electrode (YSI 5331) (Dubinsky et al., 1987). Measurements were made in a double-walled, cylindrical 230-m1 chamber filled with filtered seawater (GF/C 0.45 [micro]m). Temperature was maintained at 24 [+ or -] 0.1[degrees]C. The water in each respirometry chamber was stirred by a magnetic bar beneath a perforated plate supporting the coral. An oxygen electrode detected the rate of oxygen decline in the chamber with an amplifier, which was connected to a data logger (Furier, Israel), coupled to an oxygen meter. Oxygen uptake was measured in the dark for 30 min, and then light intensity was increased, in steps of 30 min, to 10, 40, 80, 120, and 200 [micro]mo1 [m.sup.-2] [s.sup.-1].

Colony surface area was measured by covering each coral colony with plasticene, then removing the cover and spreading it flat. The outline of the plasticene sheet was then copied onto aluminum foil and cut; surface area was determined by the weight of the foil in relation to the weight of a piece of foil of known surface area (Marsh, 1970). For calculation of light compensation point ([I.sub.com]), we used computer software attached to the data logger, and the inverse quadratic equation for unit surface area, as described by Ben-Zion and Dubinsky (1988).

Results

Coral expansion behavior

The expansion behavior of F. favus colonies varied with each combination of stimuli. In the first set of experiments at low flow speed (5 cm [s.sup.-1]) under low irradiance levels (40 [micro]mol [m.sup.-2][s.sub.-1]), the coral tentacles expanded only partially with or without the addition of Artemia nauplii (Fig. 2A), up to level 1 (25%) (Fig. 1). Most tentacles remained fully contracted under medium irradiance level (Fig. 2B); when prey were added, only one coral responded and expanded up to level 1 (25%). In the control tanks with no water flow, the corals remained contracted during the entire 180 min of the experiment. When corals were exposed to higher irradiance (120 [micro]mol [m.sub.-2][s.sup.-1]), tentacle expansion did not occur at all, and the corals remained contracted during the entire experiment (180 mm) (Fig. 2C). The same response occurred in the two control tanks in which corals were exposed to no flow at 120 [micro]mol [m.sup.-2][s.sup.-1].

In the second set of experiments at intermediate water flow rate (10 cm [s.sup.-1]), at a low light intensity of 40 [micro]mol [m.sup-2] [s.sup.-1], and in the absence of Artemia nauplii, two corals expanded up to level 1 (25%). When Artemia nauplii were added, four corals expanded their tentacles--two to level 1 (25%) and two up to level 2 (50%) (Fig. 3A). In the control tanks with no flow, all corals remained contracted during the entire experiment, even when prey was added. When light intensity was increased to 80 .[micro]mol [m.sup.-2] [s.sup.-1], only three corals responded. Two colonies Out of the group fed with Artemia expanded to level 3 (75%); the third colony from the unfed group expanded only to level 1 (25%) (Fig. 3B). No expansion responses occurred in the two control groups at 80 [micro]mol [m.sup.-2] [s.sup.-1]. At high light intensity (120 [micro]mol [m.sup.-2] [s.sup.-1]) and intermediate flow speed, only four corals expanded their tentacles slightly, two in the Arternia-fed group and two in the non-fed group (Fig. 3C). Again, there was no expansion response by the control corals.

In the third set of experiments run at high flow speed (15 cm [s.sup.-1]), with low irradiance (40 [micro]mol [m.sup.-2] [S.sup.-1]), most of the corals showed positive response and expanded their tentacles. In the group fed with Artemia, four corals responded: two fully expanded to level 4 (100%), and two opened to level 3 (75%).

In the non-fed group, four corals responded: two expanded to level 1 (25%), and the other two expanded up to level 2 (50%) (Fig. 4A). In the two control groups, with no flow, the corals remained contracted during the entire experiment. Similar responses occurred at medium light intensity 80 )[micro]mol [m.sup.-2] [s.sup.-1] (Fig. 4B), but no expansion response occurred in the two (no-flow) control groups. Only one coral expanded its tentacles to level 3 (75%) when light irradiance was increased to 120 [micro]mol [m.sup.-2] [s.sup.-1], and all the corals, including those in the other control groups, remained contracted during the entire 180 min (Fig. 4C).

A three-way ANOVA of expansion behavior with the three variables tested (light, flow, and prey) revealed no significant interactions between the variables (P [less than] 0.0001 [F.sub.(17,89)] = 3.54) (Table 1). A three-way ANOVA without interactions showed significant variation in expansion with each variable (P [less than] 0.000 1, [F.sub.(584)] = 7.27) (Table 2). Duncan's test (Table 3) showed significantly higher frequencies of coral expansion at high flow (15 cm [s.sup.-1]) than at low (5 cm [s.sup.-1]) and medium flow (10 cm [s.sup.-1]). Expansion rates also were significantly higher at low-to-medium irradiance levels (40 and 80 [micro]mol [m.sup.-2] [s.sup.-1]) than at high irradiance (120 [micro]mol [m.sup.-2] [s.sup.-1]). When prey (Artemia) was present, expansion rates were significantly higher than for corals not fed with prey (Table 3).

Light compensation point ([I.sup.com])

Calculation of the P/I curve revealed that the irradiance level needed to achieve compensation in F. favus was 107 [+ or -] 24 [micro]mol [m.sup.-2] [s.sup.-1] (n = 5 corals tested). We calculated only the [I.sub.com]' therefore the maximum light intensity that the corals were exposed to was only 200 [micro]mol [m.sup.-2] [s.sup.-1].

Discussion

We demonstrate here the relative importance of light, flow, and zooplankton prey in controlling the expansion behavior of a stony coral. Although previous studies have examined these factors separately, the present study is the first to test all three factors simultaneously and to elucidate that prey presence is of secondary importance to light and flow in inducing the expansion of coral tissue.

Several reports have demonstrated that stony corals and sea anemones expand their tentacles in the presence of food (Mariscal and Lenhoff, 1968; Reimer, 1970, 1971; Lewis and Price, 1975). Electrophysiological studies using suction electrodes in the coral Meandrina meandrites have shown that repeated stimulation of the nerve net evokes oral disk expansion and tentacle extension, similar to the expansion behavior that normally occurs at night when the corals feed (McFarlane, 1978). It is assumed that the nervous responses occur due to prey stimulation of chemoreceptors, as described for the mushroom coral Heliofungia actiniformis by McFarlane and Lawn (1991). Flow also is known to cause expansion by corals; high flow speed increases both the potential for encountering plantonic prey and the mass transfer rate of dissolved gases and nutrients (Sebens et al., 1997).

Our analysis of three variables simultaneously shows that each factor has an impact on the expansion behavior of F. favus, but that flow and light are the main triggers. Maximum tentacle expansion in F. favus corals occurred with high flow, low irradiance, and the addition of prey (Fig. 4A). When there was no addition of prey, but light intensity remained low and flow velocity high, the corals responded and expanded their tentacles up to 75%. Positive responses also occurred in medium flow, with medium light (Fig. 3A, B), but there was almost no response at all when light intensity was maximal (120 [micro]mol [m.sup.-2] [s.sup.-1]), regardless of flow speed or prey presence.

At high flow, the benefits of flow to photosynthesis are greater than the costs associated with increased respiration, thus energy available to the coral for growth and reproduction is greater, up to some asymptote, with increasing flow speed (Patterson et al., 1991; Patterson, 1992). Coral energetics depend upon tissue expansion state, which alters both the surface area exposed to flow and the thickness of the tissue layers through which materials diffuse. In high-speed flow environments, flow modulation of coral energetics is minimal. When water flow is low, dense branches or other morphologies that increase the total volume and thickness of a colony's diffusional boundary layer may negatively affect the coral's energy balance. In low-flow environments, the diffusional boundary layer may become severely limiting to metabolic processes; corals in these environments show morphologies that maximize the exposure of their tissue surfaces to flow (Lesser et al., 1994; Sebens et al., 1997).

We suggest that expansion behavior in zooxanthellate corals relates to their energy equilibrium between heterotrophy and autotrophy. The expanded tentacles of F.favus corals have low densities of zooxanthellae and may produce a self-shadow on the rest of the coral tissue that contains dense algae (O. Levy, unpub. obs.). Thus, under conditions of high light, expansion may lead to a decrease in overall photosynthetic rate and an increase in coral metabolic rate, resulting in a net loss of energy.

The expansion responses that we observed here lead us to propose a hierarchy of response to the three variables examined. According to our results, in still water, corals will not expand their tentacles even if light and prey levels vary (Fig. 5). When the light level is too high (over [I.com]), they also will not expand, even when flow and prey vary. When light levels are low ([less than][I.sub.com]), the expansion behavior depends on conditions of flow and prey (Fig. 5). Finally, if no prey is present, the corals will still expand, but only if light is low and flow speed is medium to high. Therefore, the coral's response to prey presence is secondary to its response to light and flow.

This relative importance of different environmental factors in controlling tentacular expansion (Fig. 5) may apply to many coral species, which, like the colonies of F. favus tested here, have low densities of algae in their tentacles and are known for their nocturnal expansion behavior. However, these results probably do not apply to corals that contain high algal densities in their tentacles and thus would benefit from expansion when light levels are high.

Acknowledgments

We thank the staff of the Interuniversity Institute for Marine Science in Eilat for hospitality and diving facilities. We acknowledge the assistance of S. Karko, D. Zakai, N. Stambler, and N. Simon-Blecher in conducting the experiments. We thank K. Tarnaruder for help with graphical design; we also appreciate the help of R. Levy with the statistical analysis.

(*.) To whom correspondence should be addressed. Email: levysher@netvision.net.il.

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                   Three-way ANOVA, with interactions
                      among the variables of light
                   intensity, prey presence, and flow
                   speed on the behavior of tentacle
                   expansion in the stony coral Favia
                          favus after 180 min
Source of variation  DF  Mean square      F                P
Light                 2      3298.61   6.55  [less than]0.01
Prey                  1      2777.77   5.52  [less than]0.05
Light X Prey          2      1173.61   2.33  Not significant
Flow                  2      5361.11  10.65            0.001
Light X Flow          4      1027.77   2.04  Not significant
Prey X Flow           2       694.44   1.38  Not significant
Light X Prey X Flow   4       590.27   1.17  Not significant
P [less than] 0.0001, [F.sub.17,89)] = 3.54.
                        Three-way ANOVA, without
                   interaction among the variables of
                  light intensity, prey presense, and
                     flow speed on the behavior of
                    tentacle expansion in the stony
                    coral Favia favus after 180 min
Source of variation  DF  Mean square   F           P
Light                2     3298.61    5.96  [less than]0.01
Prey                 1     2777.77    5.02  [less than]0.005
Flow                 2     5361.11    9.69  [less than]0.001
P [less than] 0.0001, [F.sub.(5,84]) = 7.27.
                     Duncan's test on the effect of
                     three types of stimuli (light
                   intensity, prey presence, and flow
                    speed) on the tentacle expansion
                      behavior of the stony coral
                              Favia favus
                             Tentacle expansion
Stimulus                          (mean %)       Duncan grouping
Light intensity ([micro]mol
[m.sup.-2] [s.sup.-1])
  40                                27.5                A
  80                               19.16                A
  120                               6.66                B
Prey (Artemia nauplii)
  Present                          23.33                A
  Absent                           12.22                B
Flow speed (cm [s.sup.-1])
  5                                 5                   B
  10                               16.66                B
  15                               31.66                A


Maximum coral expansion occurred when light intensity was low (40 [micro]mol [m.sup.-2] [s.sup.-1]), prey were present, and water flow speed was high (15 cm [s.sup.-1]. See Fig. 1 for description of % tentacle expansion. Means with the same letter are not significantly different a P = 0.05.

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Author:LEVY, O.; MIZRAHI, L.; CHADWICK-FURMAN, N. E.; ACHITUV, Y.
Publication:The Biological Bulletin
Date:Apr 1, 2001
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