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Exploration of the sea urchin coelomit fluid via combinatorial peptide ligand libraries.

Introduction

Sea urchin is a widely used model organism for the study of different features in cellular, molecular, and developmental biology (Gustafson and Wolpert, 1963; Giudice, 1986; Nishioka et al., 2003). Coelomic fluid is a suspension that occupies the coelomic cavity of these invertebrates. The general function of such fluid is both to wrap and protect the inner organs and to mediate interactions between different regions of the coelomic cavity. A large number of cells (1-5 X 106/ml), called coelomocytes, are contained in the coelomic fluid and take part in the sea urchin immune response (Smith et at, 2006). About 1/3 of these cells are involved in phagocytosis, while the remainder have clotting and antibacterial functions through the release of specific molecules (Smith et a/.,1996; Stabili et at, 1996; Terwilliger et at, 2006; Dheilly et al., 2009).

The genome of the sea urchin Strongylocentrotus purpurants has been recently sequenced (Sea Urchin Genome Sequencing Consortium et at, 2006), primarily because of its usefulness as a model research system for molecular, evolutionary, and cell biology. Its genome has been found to contain about 23,300 genes. The interest in this organism in cell biology stems primarily from (a) its diverse and sophisticated immune system, mediated by an extraordinarily large repertoire of innate pathogen-recognition proteins; (b) the presence of orthologs of genes associated with vision, hearing, and balance; and (c) the presence of orthologs of many genes associated with human disease.

Not much has been published so far on the proteome of sea urchin. In 2007, Noll et al. reported an extensive investigation on the toposome, a large, hexameric glycoprotein essential for cell adhesion and development. More recently, reports have appeared on the expression of selected proteins, such as 14-3-3 epsilon and Plnectin, in sea urchin embryos (Russo et at, 2010; Zito et at, 2010). The sea urchin is also a well-established animal model for studying pollutant toxicity, such as that derived from heavy metals (Matranga and Yokota, 2008; Pinsino et al., 2010). Of the very few proteomic studies on sea urchin, one investigated the response of coelomocytes to bacterial infection. Upon two-dimensional map analysis and spot excision, 17 proteins, whose expression had been modulated by the infectious agent, could be identified (Dheilly et al., 2011). Perhaps the most extensive proteome investigation in sea urchins is represented by the work of Mann et al. (2010), who examined the proteome content of the spicules (calcitic skeletal elements), which abound in sea urchin embryos as development to the pluteus larva proceeds. Since most of the proteins embedded in the matrix of coelomic fluid would contain transmembrane domains and would thus be difficult to solubilize, the authors resorted to total trypsin digestion as a solubilizing step. This permitted, via mass spectrometry (MS) analysis, the identification of 231 unique gene products.

Since the genome of Strongylocentrotus purpuratus, the purple sea urchin, was sequenced in 2006 (Sea Urchin Genome Sequencing Consortium et al., 2006), only a few studies (Mann et al., 2008; Sewell et al., 2008) have investigated the proteome of coelomic fluid in echinoderms. In the coelomic fluid the cells responsible for immuno-defense, the coelomocytes, are dispersed, and one problem for knowledge of its proteome is the low protein level. In the present study we have applied the combinatorial peptide ligand library (CPLL) technology to the enhancement and normalization of relative concentrations of coelomic proteins, so as to explore in depth the proteome of coelomic fluid. CPLLs (see Righetti et al., 2006, 2010; Righetti and Boschetti, 2008; for reviews) have worked very well in exploring the low-abundance proteome in a variety of biological fluids, in particular in the analysis of the red blood cell cytoplasmic proteome (Roux-Dalvai et al., 2008) and human plasma (Sennels et al., 2007) and cerebrospinal fluid (Mouton-Barbosa et al., 2010) proteomes. Here we report the coverage of the sea urchin coelomic fluid.

Materials and Methods

Chemicals, biologicals, and apparatuses

ProteoMiner (combinatorial hexapeptide ligand library beads, PM), molecular mass standards, SDS-PAGE precast gels, Dc protein assay kit and electrophoresis apparatus for one-dimensional electrophoresis were from Bio-Rad Laboratories (Hercules, CA). [bate]-mercaptoethanol, DTT, De-Streak [Bis-(2-hydroxyethyl)disulphide, [(HOC[H.sub.2]C[H.sub.2]).sub.2][S.sub.2])], and all other chemicals used throughout the experimental work were current pure analytical grade products purchased from Sigma-Aldrich (St. Louis, MO). Complete protease inhibitor cocktail tablets was from Roche Diagnostics (Ba-sel, CH). Homemade combinatorial peptide ligand libraries (HM-CPLL) were produced in our laboratory and used as described in Cereda et al. (2010).

Coelomic: fluid collection and treatment

As sea urchin coelomic fluid is similar in its ionic composition to the outside environment, we collected our sampies of Paracentrotus lividus (Lamarck) from different locations to counterbalance possible differences in the composition of the fluid due to different microenvironments. Coelomic fluid was collected from different individuals, through the peristomium, using a 10-m1 syringe with a 21-gauge needle. The collected fluid was pooled, an isosmotic, anti-coagulant solution (ISO-EDTA: 20 mmol [1.sup.-1] Tris, 0.5 mol [1.sup.-1] NaCI, 70 mmol [1.sup.-1] EDTA, pH 7.5) was added, and the liquid phase was separated from the cellular component by centrifugation at 17400 X g for 5 min in a Beckman centrifuge using a JA20 rotor (the sample pro-cessed here is referred to as cell-free coelomic fluid). The liquid phase was dialyzed overnight at 4 [degrees] C against distilled water, and then lyophilized in a speed vacuum centrifuge. Aliquots of 300 mg of lyophilized sample were resuspended in 25 mmol [1.sup.-1] phosphate buffer, pH 7.2, and 50 [micro]l of PM beads subsequently added. Protein adsorption was imple-mented via gentle shaking for 3 h at room temperature; then the beads were collected by filtration and washed twice with 10 mmol [1.sup.-1] phosphate buffer at pH 7.2. The flow-through was then titrated to pH 2.2 and a further 50 AI of HM-CPLL was added for additional capture of species that might not have been adsorbed at pH 7.2. The captured proteins from each peptide library sample were then desorbed by using a solution composed of 4% SDS, 2% DTT for 15 min, under boiling conditions (as in Candiano et at, 2009). Desorption was carried out twice with 50 [micro]l each, and the two eluates were pooled. Such extractions were done in triplicate. Two different biological samples were prepared separately and analyzed separately by nano-liquid-chromatography-mass spectrometry.

SDS-poivacrylatn ide gel electrophoresis

The gel was loaded with 15 or 20 pA of each bead eluate and control, in the presence of Laernmli buffer (4% SDS. 20% glycerol, 10% 2-mercaptoethanol. 0.004% broniophenol blue, and 0.125 mol [1.sup.-1] Tris MCI, pH approx. 6.8. The SDS-PAGE gel was composed of a 4% poiyacrylamIde stacking gel (125 mmol [1.sup.-1] Tris-HC1, pH 6.8, 0.1% SDS) cast over an 8%-18% resolving polyacrylamide gel (in 375 mmol [1.sup.-1] Tris-HC1, pH 8.8, 0.1% SDS buffer). The cathodic and anodic compartments were filled with Tris-glycine buffer, pH 8.3, containing 0.1% SDS. Electrophoresis was at 100 V until the dye front reached the bottom of the gel. Staining and destaining were performed with colloidal Coomassie blue and 7% acetic acid in water, respectively (Candiano et aL, 2004). The SDS-PAGE gels were scanned with a Model 300 VersaDoc imaging system (Bio-Rad).

Two-dimensional map analysis

After SDS content was eliminated by precipitation with methanol/chloroform, the desired amount of protein sample was solubilized in 2D sample buffer (7 mol [1.sup.-1] urea, 2 mol [1.sup.-1] thiourea, 3% CHAPS, 40 mmol [1.sup.-1] Tris) to a final concentration of 2 mg/m1 protein. Disulfide bond reduction was performed at room temperature for 60 min by addition of TCEP [Tris(2-carboxyethyl)phosphine hydrochloride] at a final concentration of 5 mmol [1.sup.-1]. For alkylating sulfy-dryl groups, De-Streak [Bis-(2-hydroxyethyl)disulphide, [(HOC[H.sub.2]C[H.sub.2]).sub.2][S.sub.2])] was added to the solution at a final concentration of 150 mmol [1.sup.-1] (by dilution from the 8.175 mol [1.sup.-1] stock solution, Sigma-Aldrich), followed by addition of Ampholine (from the 40% stock solution to a final concentration of 0.5%) and a trace amount of bromophenol blue. IPG strips (Bio-Rad), pH 3-10 nonlinear, 18-cm long, were rehydrated with 150 [micro]l of protein solution for 5 h. Isoelectric focusing was carried out in a linear voltage gradient from 100 to 2000 V for 5 h, 2000 V for 4 h, followed by an exponential gradient up to 10000 V, until each strip was electrophoresed for 25 kV/h. For the second dimension, the IPG strips were equilibrated for 25 min in a solution containing 6 mol 1-1 urea, 2% SDS, 20% glycerol, 375 mmol [1.sup.-1] Tris-HC1 (pH 8.8) under gentle shaking. The 1PG strips were then laid on an 8%-18% acrylamide gradient SDS-PAGE with 0.5% agarose in the cathode buffer (192 mmol [1.sup.-1] glycine, 0.1% SDS and Tris-HC1 to pH 8.3). The electrophoretic run was performed by setting a current of 5 mA/gel for 1 h, followed by 10 mA/gel for 1 h and 15 mA/gel until the dye front reached the bottom of the gel. Gels were incubated in a fixing solution containing 40% methanol and 7% acetic acid for 1 h, followed by silver staining (Shevchenko et al., 2006). Destaining was performed in 7% acetic acid until background was clear, followed by a rinse in pure water. The 2-DE gels were scanned with the VersaDoc imaging system (Bio-Rad), by fixing the acquisition time at 10 s; the relative gel images were captured via the PDQuest Advanced software, ver. 8.0 (Bio-Rad). After the gel images were filtered to remove the background, spots were automatically detected, manually edited, and then counted.

Mass spectrometry and data analysis

The sample lanes of SDS-PAGE were cut in eight pieces along the migration path and proteins were reduced by 10 mmol [1.sup.-1] DTT, alkylated by 55 mmol [1.sup.-1] iodoacetamide, and digested overnight with 0.1 mg/m1 bovine trypsin 99% grade (Shevchenko et al., 2006). The tryptic mixtures were acidified with formic acid to a final concentration of 1%. Ten microliters of tryptic digest for each band was injected in a nano-chromatographic system, UltiMate 3000 RSLCnano System (Dionex). The peptide mixtures were loaded on a reversed-phase trap column for the cleanup and pre-concentration. After cleanup, the two-position valve was switched to place the trap column in series with a fused silica reversed-phase column (Acclaim PepMap100, C18, 3 [micro]m, 100, 75 [micro]m i.d. x 15 cm, Dionex). The peptides were eluted with a 30-min gradient from 4% buffer A (2% acetonitrile, 0.1% formic acid in water) to 60% buffer B (2% water, 0.1% formic acid in acetonitrile) at a constant flow rate of 300 ml/min. The liquid chromatograph was connected to an LTQ-XL mass spectrometer (Thermo Scientific) equipped with a nanospray ion source. Fullscan mass spectra were acquired in the mass range ink 350 to m/z 1800 Da, and the three most intense ions were automatically selected and fragmented in the ion trap. Target ions already selected for mass spectrometry (MS/MS) were dynamically excluded for 60 s. The MS data were searched against three databases with the Mascot search engine (ver. 2.3.01): Uniprot_sea urchin (1058248 residues, 3418 sequences) and Uniprot_ EST_sea urchin (409520196 residues, 1720644 sequences), and a non-redundant protein database from Blast. The first two databases were down-loaded from the National Center for Biotechnology Information (http://www.ncbi.nlm.nih.gov/nucest/?term=SEA%2OURCIN) and The Universal Protein Resource (http://www.uniprot.org/uniprot/?query=SEA+URCHIN&sort=score) in February 2011. The raw data coming from the biological duplicates of the same sample (eluate or untreated sample) were analyzed in the same batch by the Proteome Discoverer software, ver. 2.0.3, using Mascot and the parameters tryptic cleavage, cysteine alkylation, oxidation of methionine residues, and acetylation of N termini as variable modifications. Parameters were a peptide mass tolerance of 1 Da, a fragment mass tolerance of 0.8 Da, and an ion score cut-off of 20 or the cut-off calculated from an expected value of 0.05. In cases of proteins identified by a single peptide, the MSMS spectra of the corresponding peptides were checked manually. The translated nucleotide sequences obtained by Mascot, querying the EST database, were searched against a non-redundant protein database by Blast (http://blast.ncbi.nlm.nih.gov/Blast.cgi) to find the homologous proteins. In addition, the raw data were also searched against a Mascot decoy database, and the false discovery rate was 0.05%. Moreover, each band of the SDS lane was analyzed separately by nLC MSMS, and the identified proteins in specific samples are listed in the last three columns of Appendix Table 1. The Gene Ontology analysis was performed by using the QuickGO interface (Binns et al., 2009).

Results and Discussion

Figure 1 shows the SDS-PAGE profiling of the sample not treated by CPLL beads (control) and of two eluates from the CPLL beads, after capture at pH 7.2 and 2.2. A strong decrement of the two heavy bands at 75 and 100 kDa, present in the control, can be observed in the CPLL eluates. In addition, the capture at pH 2.2 seems to be particularly enriched in low Mr components, ranging from 10 to 20 kDa. There is a heavy vertical streaking in all lanes, which we could not abolish by treating the sample with DNA-ase and not even with PNGase, which should eliminate the glycan moiety in heavily glycosylated species such as the topo-some, one of the most abundant proteins in this biological fluid (not shown). The enrichment in low-abundance species can also be appreciated in the 2D maps of Figure 2, the left one showing the control and the right one a pool of the two eluates (after elimination of excess SDS, as in Candiano et at, 2009).

The lanes in Figure 1 were cut into 8 segments (7 for the p1-1 2.2 lane). The proteins were digested with trypsin and identified via MS analysis. The Venn diagram on the right side summarizes the total findings (82 proteins identified), among which only 26 could be detected in the control fluid, all else being attributed to the two eluates. Although the increment in detection is greater than 3-fold, the total protein discovery is not so high, summing to a total of 82 unique gene products. Judging from the 2D maps of all eluates (Fig. 2, right panel), one would expect that a much greater number of proteins would be identified. Yet the total number of spots in this panel is misleading in that one can observe a large presence of horizontal strings of spots. This geometry suggests the presence of charge-modified species that is, those originating from deamidation, sulfation, acetylation, or glycosylation or glycation, as theoretically predicted and experimentally verified by Pietrogrande et al. (2005). Such post-synthetic modifications could generate an impressive number of spots from individual polypeptide chains. As an example, in human cerebrospinal fluid, the prion protein, which is composed of three polypeptide chains of mass values from 18 to 23 kDa, just due to the presence of two glycosylation sites, produces a total of 64 spots in 2D maps (Castagna et al., 2002).

Appendix Table 1 lists all the species so far identified: since the control was characterized by 26 new gene products, the CPLL treatment of coelomic fluid has permitted us to identify 82 new proteins. This suggest that our method could be useful for the enrichment of low-abundance proteins. In fact, the gene ontology (Fig. 3) also showed differences between treated and control samples in the percentages of proteins in various categories of proteins. The first four categories in the CPLL eluates are extracellular region (13%), DNA binding (12%), ATP binding (10%), and cytoplasm (9%); whereas in the control they are cytoplasma (13%), ATP binding (12%), extracellular region (10%), and membrane (9%).

But considering the general functions of identified proteins, our data have confirmed the complexity of molecular pathways that have been reported in the literature (Franco a at, 2011). For example, in eluates we found proteins involved in the regulation of cell adhesion and cytoskeleton organization: the actin cytoskeletal (gi/10939908) and profilin (gi/89440162). The last one is also important for changes in the cell shape of sea urchin coelomocytes (Smith et al., 1992). Moreover, calmodulin 2 (gi/139254559), identified in the eluate samples, is a C[a.sup.2+]-binding protein involved in the signaling events because calcium intracellular concentration regulates cellular signals. A protein important in the regeneration processes was always identified in the eluates: the wnt8 (gi/61136150). Finally, the complement C3 (gi/89438622) was found both in the eluates and in the control, proving the presence of nonspecific innate immune response in sea urchin.

Our identifications were important not only to give a preliminary proteomic characterization of coelomic fluid of sea urchin, but also to test the capability of CPLL to deplete the abundant proteins. In fact, in the control coelomic fluid we found two very abundant proteins: the toposome and the major yolk proteins, both members of a new class of cell adhesion proteins. In particular, the toposome is a modified calcium-binding, iron-less transferrin that in denaturing conditions (SDS-PAGE) generates different bands along the migration track. Moreover, this protein is highly glycosylated at the N-terminus. This particular feature favored a stronger binding of the toposome to the beads. As one can see in Appendix Table 1, we found this protein with a very high score in the CPLL eluates (38150) and with a diminished score, but still quite high, in the control (5861). Due to this rather overwhelming presence, one can also find peptides originating from the toposome protein in other bands along the individual gel lanes. Another undesirable aspect is that the overpowering presence of the toposome might have inhibited binding of many other proteins to the beads and in turn brought about a suppression of signals during mass spectrometry.

In conclusion, the present work has unraveled the identity of several proteins not previously reported in coelomic fluid. This will help in decoding the biological function of this fluid; nevertheless, more efforts will have to be devoted in the future to identifying other very low abundance proteins that will surely be present in coelomic fluid.

Acknowledgments

PGR is supported by MURST, Rome, Italy (PR1N 2008, project number 20087ATS57). We dedicate this work to Professor Rainer Barbieri, one of the authors, who died prematurely after this article was submitted. The co-authors remember that he was the true inspirer and promoter of this research.

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Received 11 July 2011; accepted 28 February 2012.

* To whom correspondence should be addressed. E-mail: daniele.bellavia@unipa.it. [double dagger]: Deceased.

Abbreviations: CPLL, combinatorial peptide ligand library; HM-CPLL, home-made combinatorial peptide ligand library; PM, ProteoMiner.

ELISA FASOLT (1), ALFONSINA D'AMATO'(1), PIER GIORGIO RIGHETTI (1), RAINER BARBIERI (2), [double dagger], AND DANTELE BELLAVIA (2), *

(1.) Department of Chemistry, Materials and Chemical Engineering "Giulio Natta", Politecnico di Milano, Via Mancinelli 7, 20131 Milan, Italy; and (2.) Dipartimento di Scienze e Tecnologie Molecolari e Biomolecolari (STEMBIO), Sezione di Biologia Cellulare, Universitcl degli Studi di Palermo, V.le delle Scienze, Edificio 16, 90128 Palermo, Italy

Appendix

Table 1 Identified proteins in celomalic fluid of sea urchin

Database       Accession       Protein name            Mascot      Mr
               number                                   score

EST_seaurchin  gi|50872995     Sp1170 Bacterially        2856   54162
                               activated coelomocyle.
                               arrayed
                               Strorgvlocetrotus
                               purpuratus cDNA clone
                               Spl170 5', similar to
                               Actin, mKNA sequence

               gi|139309108    AM569285 RZPD             2716   32092
                               MPMGp1173
                               Paracentrotus lividus
                               CASPLU Paracentrotus
                               lividus cDNA clone
                               MPMGp1173C12115Q 5',
                               mRNA sequence

               gi|109400265    132678021 Sea Urchin      1793   38193
                               Mesentary cDNA library
                               Strongylocentrouce
                               purpuratus cDNA done
                               MSP-2L19 5', mRNA
                               sequence

               gi|24901528     RN.V/8T3 Sea urchin       1537   46192
                               radial nerve Lambda
                               Zap cDNA library
                               Heliocidaris
                               ervthrogramma cDNA 5',
                               similar to Cyl actin,
                               mRNA

               gi|5713384      MPMGp621_0341119          1475   37539
                               MPMGp621
                               Strongylocentrouce
                               purpuratus to cDNA
                               done 034H19 similar to
                               CyIIIa. mRNA sequence

               gi|89438622     AM2(MW54 RZPD             1051   17060
                               MPMGp1172
                               Paracentrotus lividus
                               MBSB Paracentrotus
                               lividus cDNA done
                               MPMGp1172J2350Q 5',
                               mRNA

               gi|139330259    AM573430 RZPD              950   26036
                               MPMGp1173
                               Paracentrotus lividus
                               GASPLU Paracentrotus
                               lividus cDNA clone
                               MPMGp1173F08126Q 5',
                               mRNA

               gi|34756695     StrPu691.005010 Sea        389   23648
                               urchin larva cDNA
                               library MPMGp691
                               Strangylocentrotus
                               purpuratus cDNA clone
                               MPMGp69101838;
                               MPI_SURUD138018 5',
                               mRNA sequence

               gi|89445358     AM2IU987 RZPD              366   26014
                               MPMGp1172
                               Paracentrotus lividus
                               MBSB Paracenlrotus
                               liudus cD.NA Clone
                               MPMGp1172I0660Q 5',
                               mRNA

               gi|89442983     AM2OH097 RZPD MPMGp1       352   32135
                               174 Paracentrotus
                               lividus EGG
                               Paracentotus lividus
                               cDNA clone
                               MPMGp1174D2214Q 5',
                               mRNA

               gi|139328625    AM563391 RZPD              350   34078
                               MPMGp1173
                               Paracentrotus lividus
                               GASPLU Puracentratus
                               lividus cDNA clone
                               MPMGp1173E09100Q 5',
                               mRNA

               gi|109399080    133334354 Sea Urchin       347   37109
                               Lantern cDNA library
                               Strangylocentrotus
                               purpuratus cDNA clone
                               LSP-2F21 5', mRNA
                               sequence

               gi|62385757     92292867 Sea Urchin        333   33809
                               primary mesenchyme
                               cell cDNA library
                               Strangylocentrotus
                               purpuralus cDNA clone
                               PMCSPR2-165P21 5',
                               mRNA

               gi|89457047     AM214690 RZPD              323   23910
                               MPMGp1174 Paracentotus
                               lividus HGG
                               Paracentotus lividus
                               cDNA clone
                               MPMGp1174E1230Q 5',
                               mRNA sequence

               gi|91196436     AM205158 RZPD              255   24959
                               MPMGp1172 Paracentotus
                               lividus MBSB
                               Paracentotus lividus
                               cD.NA clone
                               MPMGp1172PI860Q 5',
                               mRNA sequence

               gi|89437392     AM188140 RZPD              250   25166
                               MPMGp1172 Paracentrous
                               lividus MBSB
                               Paracentrous lividus
                               cDNA clone
                               MPMGp1172D171Q 5',
                               mRNA sequence

               gi|109399528    133334291 Sea Urchin       236   28186
                               Lantern cDNA library
                               Strongylocentrouce
                               purpuratus cDNA clone
                               LSP-2B1 5', mRNA
                               sequence

               gi|139265663    AM570670 RZPD              218   31335
                               MPMGp1173 Paracentrous
                               lividus GASPI.U
                               Paracentrous lividus
                               cDNA clone
                               MPMGp1173O02118Q 5',
                               mRNA sequence

               gi|139245731    AM557988 RZPD              214   35260
                               MPMGp1172 Paracentrous
                               lividus MBSB
                               Paracentrous lividus
                               cDNA clone
                               MPMGp1172M1164Q 5',
                               mRNA sequence

               gi|139268548    AM551774 RZPD              162   30182
                               MPMGp1172 Paracentrous
                               lividus MBSB
                               Paracentrous lividus
                               cDNA clone
                               MPMGp1172P17105Q 5',
                               mRNA sequence

               gi|139302769    AM539511 RZPD              158   28010
                               MPMGp1171 Paracentrous
                               lividus CLEB
                               Paracentrous lividus
                               cDNA clone
                               MPMGp1171E03207Q 5',
                               mRNA sequence

               gi|89446177     AM212199 RZPDMPMGp1174     138   25419
                               Paracentrous lividus
                               EGG Paracentrous
                               lividus cDNA clone
                               MPMGp1174G0224Q 5'
                               mRNA sequence

               gi|34746608     StrPu691.006926 Sea        135   27274
                               urchin larva cDNA
                               library MPMCp69l
                               Strongylocentrouce
                               purpuratus cDNA clone
                               MPMGp69101599; MPI
                               SURUDI_99015 5', mRNA
                               sequence

               gi|89440162     AM197614 RZPD              135   26921
                               MPMGp1172 Paracentrous
                               lividus MBSB
                               Paracentrous lividus
                               cDNA clone
                               MPMGp1172J1442Q 5'
                               mRNA sequence

               gi|139242372    AM545798 RZPD              134   26167
                               MPMGp1171 Paracentrous
                               lividus CLEB
                               Paracentrous lividus
                               cDNA clone
                               MPMGp1171F22200Q 5'
                               mRNA sequence

               gi|89438467     AM197503 RZPD              129   32029
                               MPMGp1172 Paracentrous
                               lividus MBSB
                               Paracentrous lividus
                               cDNA clone
                               MPMGp1172HI642Q 5',
                               mRNA sequence

               gi|l39268897    AM543994 RZPD              120   32698
                               MPMGp1171 Paracentrous
                               lividus CLEB
                               Paracentrous lividus
                               cDNA clone
                               MPMGpll71E13116Q 5',
                               mRNA sequence

               gi|91194567     AM194337 RZPD              119   26742
                               MPMGp1172 Paracentrous
                               lividus MBSB
                               Paracentrous lividus
                               cDNA clone
                               MPMGp1172L1134Q 5',
                               mRNA sequence

               gi|139255468    AM566312 RZPD              115   32171
                               MPMGp1173 Paracentrous
                               lividus GASPLU
                               Paracentrous lividus
                               cDNA clone
                               MPMGp1173C14108Q 5',
                               mRNA sequence

               gij 109401942   165424028 Sea Urchin       112   32556
                               Radial Nerve cDNA
                               Library
                               Strongylocentrouce
                               purpuratus cDNA clone
                               RNSP-9D11 5', mRNA
                               sequence

               gi|139313404    AM591951 RZPD              107   31793
                               MPMGpI173 Paracentrous
                               lividus GASPU.
                               Paracentrous lividus
                               cDNA clone
                               MPMGp1173E1357Q 5',
                               mRNA sequence

               gi|139357079    AM52S425 RZPD MPMGpl       100   29368
                               171 Paracentrous
                               lividus CLEB
                               Paracentrous lividus
                               cDNA clone
                               MPMGp1171.107203Q 5',
                               mRNA sequence

               gi|34745864     SirPuMI.006182 Sea          89   25783
                               urchin larva cDNA
                               library MPMGp691
                               Strongylocentrouce
                               purpuratus cDNA clone
                               MPMGp691L1867;
                               MPI_SURUDI_67L18 5',
                               mRNA sequence

               gi|34757996     StrPu621.003311 Sea         88   19961
                               urchin unfertilised
                               egg cDNA Library
                               MPMGpo21
                               Strongylocentrouce
                               purpuratus cDNA clone
                               MPMGp621K2014;
                               MPI_621_14K20 5',
                               mRNA

               gi|139269763    AM527561 RZPD               87   32942
                               MPMGpll7l Paracentrous
                               lividus CLEB
                               Paracentrous lividus
                               cDNA clone
                               MPMGp117G05205Q 5',
                               mRNA sequence

               gi|57947733     ydf02cO3.yl Sea urchin      87   24117
                               EST Lib 1
                               Sironevlocentrotu
                               purpural us cDNA clone
                               ydf02c03 5', mRNA
                               sequence

               gi|34748064     SlrPu691.008382 Sea         86   22841
                               urchin larva eDNA
                               library MPMGp69 1
                               Strongylocentrouce
                               purpuratus cDNA clone
                               MPMGp691K04142:
                               MPI_SURUDI_142K4 5',
                               mRNA sequence

               gi|139249974    AM560126 RZPD               84   33449
                               MPMGp1172
                               Paracentrotus lividus
                               MBSB Paracentrotus
                               lividus. cDNA clone
                               MPMGp1172U870Q 5',
                               mRNA sequence

               gi|138478028    AM504745 RZPD               79   30265
                               MPMGp1171 Paracentrous
                               lividus CLEB
                               Paracentrous lividus
                               cDNA clone
                               MPMGp1171G242Q 5',
                               mRNA sequence

               gi|89442847     AM211931 RZPD               75   28495
                               MPMGp1174 Paracentrous
                               lividus KGG
                               Paracentrous lividus
                               cDNA clone
                               MPMGp1174J1323Q 5',
                               mRNA sequence

               gi|139279753    AM587839 RZPD               74   28997
                               MPMGp1173 Paracentrous
                               lividus GASPL.U
                               Paracentrous lividus
                               cDNA clone
                               MPMGp1173D1547Q 5',
                               mRNA sequence

               gi|139251073    AM568303 RZPD MPMGpl        73   30442
                               173 Paracentrous
                               lividus GASPLU
                               Paracentrous lividus
                               cDNA clone
                               MPMGp11731114112Q 5',
                               mRNA sequence

               gi|5713390      MPMGp621_035K08             72   34796
                               MPMGp621
                               Strongylocentrouce
                               purpuratus cDNA done
                               035KOK similar to
                               alpha-tubulin isotype
                               5', mRNA sequence

               gi|61136150     94026114 Sea Urchin         72   31872
                               primary mesenchyme
                               cell cDNA library
                               Strongylocentrouce
                               purpuratus cDNA clone
                               PMCSPR2-149L21 5',
                               mRNA sequence

               gi|l39254559    AM546I96 RZPD               67   26521
                               MPMGp1171 Paracentrous
                               lividus CLEB
                               Paracentrous lividus
                               cDNA done
                               MPMGp1171J06214Q 5',
                               mRNA sequence

               gi|139345636    AM542633 RZPD               63   30433
                               MPMGp1171 Paracentrous
                               lividus CLEB
                               Paracentrous lividus
                               cDNA clone
                               MPMGpl17IL17I09Q 5',
                               mRNA sequence

               gi|34751766     StrPu691.009QS4 Sea         61   22797
                               urchin larva cDNA
                               library MPMGp691
                               Strongylocentrouce
                               purpuratus cDNA clone
                               MPMGp691H4106:
                               MPI_SURUDI_106F14 5',
                               mRNA sequence

               gi|109396781    132763544 Sea Urchin        60   36079
                               Mesentary cDNA library
                               Strongylocentrouce
                               purpuratus cDNA clone
                               M.SP-1N8 5', mRNA
                               sequence

               gi|139253277    AM56264I RZPD MPMGpl        60   29133
                               173 Paracentrous
                               lividus GASPLU
                               Paracentrous lividus
                               cDNA clone
                               MPMGp1173F112Q 5',
                               mRNA sequence

               gi|l.39294700   AM579100 RZPD               58   22388
                               MPMGp1173 Paracentrous
                               lividus s GASPLU
                               Paracentrous lividus
                               cDNA clone
                               MPMGp1173.I2325Q 5',
                               mRNA sequence

               gi|61232318     92891311 Sea Urchin         56   38294
                               primary mesenchyme
                               cell cDNA librarv
                               Strongylocentrouce
                               purpuratus cDNA clone
                               PMCSPR2-73K21 5', mRNA
                               sequence

               gi|62377095     90959498 Sea Urchin         56   38138
                               primary mesenchyme
                               cell cDNA librarv
                               Strongylocentrouce
                               purpuratus cDNA clone
                               PMCSPR2-98N6 5', mRNA
                               sequence

               gi|62383655     92283899 Sea Urchin         56   36549
                               primary mesenchyme
                               cell cDNA librarv
                               Strongylocentrouce
                               purpuratus cDNA clone
                               PMCSPR2-164M9 5', mRNA
                               sequence

               gi|109399379    133201928 Sea Urchin        54   39426
                               Lantern cDNA library
                               Strongylocentrouce
                               purpuratus cDNA clone
                               LSP-1B23 5', mRNA
                               sequence

Uniprot_seaur  tr|Q6WQT5|      Toposome OS =            38158  155336
chin           Q6WQT5_PARU     Paracentrous lividus

               tr|Q3YL94|      Major yolk protein OS    21366  155959
               Q3YL94_STRNU    = Strongylocentrouce
                               purpuratus

               tr|Q2637S]      CyI actin OS =            5684   42178
               Q26378_TRIGR    Tripneustes gratilla

               tr|O18554|      Cytoplasmic actin CyII    5148   40484
               O18554_HELER    (Fragment) OS =
                               Heliocidaris
                               erythrogramma

               sp|P53474|      Actin.cytoskeletal 3A     5100   42162
               ACTE_STRPU      OS =
                               Strongylocentrouce
                               purpuratus

               tr|O18555|      Cytoplasmic actin CyII    5040   40237
               O18555_HELER    (Fragment) OS =
                               Heliocidaris
                               erythrogramma

               tr|Q8WQY0|      Toposome OS =             4937  156291
               Q8WQY0_TRIOR    Tripneustes gratilla

               tr|O18553|      Cytoplasmic actin CyII    4160   40263
               O18553_HFLTB    (Fragment) OS =
                               Heliocidaris
                               erythrogramma

               sp|Q07903|      Actin.cytoskeletal 2A     1098   42059
               ACTC_STRPU      OS =
                               Strongylocentrouce
                               purpuratus

               tr|Q70JA0|      Nectin OS =                701  108817
               Q70JA0_PARLI    Paracentrous lividus

               tr|O44344|      Complemenl component       563  187929
               O44344_STRFU    C3 OS =
                               Strongylocentrouce
                               purpuratus

               tr|Q86RA9|      Amassin OS =               287   57012
               Q86RA9_STRPU    Strongylocentrouce
                               purpuratus

               tr|A9LYL6|      Putative uterine-ovary     180   45789
               A9LYL6_HELTB    speeilie-44 protein OS
                               = Heliocidaris
                               tuberculata

               tr|Q6PTFl|      Fructose- bisphosphatc     171   21958
               Q6PTF1_STRPU    aldolase (Fragment) OS
                               = Strongylocentrouce
                               purpuratus

               tr|D5H3J3|      Ubiquitin OS =             154   14927
               D5H3J3_P5AMI    Psammechinus miliaris

               sp|P02290|      Histone H2B.1, sperm       152   16135
               H2BS1_PARAN     OS = Parechinus
                               angulosus

               tr|Q6TS36|      Neural alfa2 tubulin       135   50845
               Q6TS36_PARLI    OS = Paracentrous
                               lividus

               tr|O61230|      G-cadherin OS =            125  305537
               O61230_LYTVA    Lytechinus variegatus

               tr|Q27753|      Histone 114 (Fragment)     108    7390
               Q27753_PSAM1    OS = Psammechinus
                               miliaris

               tr|Q68BMl|      Outer arm dynein            96   62847
               Q68BMLANTCR     binding protein OS =
                               Anthocidaris

               sp|Q05634|      Fascin OS =                 71   55537
               FASC_STRPU      Strongylocentrouce
                               purpuratus

               lr|Q8MPK6|      Beta- thymosin OS -         47    4592
               QKMPK6 PARLI    Paracentrous lividus

               tr|Q963T2|      Early historic H3           44    8264
               Q963T2_9ECHN    (Fragment) OS =
                               Tetrapygus mger

               Lr|B6DS92|      Fyn-related kinase OS       43    6029
               B6DS92_STRPU    = Strongylocentrouce
                               purpuratus

               trlQSMXBO|      DNA- binding protein        41   12474
               Q8MXB0_9HCHN    HOX10 (Fragment) OS =
                               Holopneustes
                               purpurescens

               trlQ25427|      USAI OS=Lytechinus          31   32843
               Q25427_LYTVA    variegatus

               sp|Q27802|      Cytoplasmic dynein 2        35  492912
               DYHC2_TRiGR     heavy chain 1 OS =
                               Tripneustes gratilla

               tr|Q6IUG6|      Axin OS - Lytechinus        30   95299
               Q6IUG6_LYTVA    variegaius

Database       Accession       Protein name                   N
               number                                  peptides

EST_seaurchin  gi|50872995     Sp1170 Bacterially            12
                               activated coelomocyle.
                               arrayed
                               Strorgvlocetrotus
                               purpuratus cDNA clone
                               Spl170 5', similar to
                               Actin, mKNA sequence

               gi|139309108    AM569285 RZPD                 12
                               MPMGp1173
                               Paracentrotus lividus
                               CASPLU Paracentrotus
                               lividus cDNA clone
                               MPMGp1173CI2I I5Q 5',
                               mRNA sequence

               gi|109400265    132678021 Sea Urchin           5
                               Mesentary cDNA library
                               .Strongylocentrouce
                               purpuratus cDNA done
                               MSP-2L19 5', mRNA
                               sequence

               gi|24901528     RN.V/8T3 Sea urchin            7
                               radial nerve Lambda
                               Zap cDNA library
                               Heliocidaris
                               ervthrogramma cDNA 5',
                               similar to Cyl actin,
                               mRNA

               gi|5713384      MPMGp621_0341119               7
                               MPMGp621
                               Strongylocentrouce
                               purpuratus to cDNA
                               done 034H19 similar to
                               CyIIIa. mRNA sequence

               gi|89438622     AM2(MW54 RZPD                  9
                               MPMGp1172
                               Paracentrotus lividus
                               MBSB Paracentrotus
                               lividus cDNA done
                               MPMGp1172J2350Q 5',
                               mRNA

               gi|139330259    AM573430 RZPD                  4
                               MPMGp1173
                               Paracentrotus lividus
                               GASPLU Paracentrotus
                               lividus cDNA clone
                               MPMGp1173F08126Q 5',
                               mRNA

               gi|34756695     StrPu69 1.005010 Sea           3
                               urchin larva cDNA
                               library MPMGp691
                               Strangylocentrotus
                               purpuratus cDNA clone
                               MPMGp69101838;
                               MPI_SURUDI 38018 5',
                               mRNA sequence

               gi|89445358     AM2IU987 RZPD                  1
                               MPMGp1172
                               Paracentrotus lividus
                               MBSB Paracenlrotus
                               liudus cDNA Clone
                               MPMGp1172I0660Q 5',
                               mRNA

               gi|89442983     AM2OH097 RZPD MPMGpl           3
                               174 Paracentrotus
                               lividus EGG
                               Paracentotus lividus
                               cDNA clone
                               MPMGp1174D2214Q 5',
                               mRNA

               gi|139328625    AM563391 RZPD                  4
                               MPMGp1173
                               Paracentrotus lividus
                               GASPLU Puracentratus
                               lividus cDNA clone
                               MPMGp1173E09100Q 5',
                               mRNA

               gi|109399080    133334354 Sea Urchin           7
                               Lantern cDNA library
                               Strangylocentrotus
                               purpuratus cDNA clone
                               LSP-2F21 5', mRNA
                               sequence

               gi|62385757     92292867 Sea Urchin            7
                               primary mesenchyme
                               cell cDNA library
                               Strangylocentrotus
                               purpuralus cDNA clone
                               PMCSPR2-165P21 5',
                               mRNA

               gi|89457047     AM214690 RZPD                  1
                               MPMGp1174 Paracentotus
                               lividus HGG
                               Paracentotus lividus
                               cDNA clone
                               MPMGp1174E1230Q 5',
                               mRNA sequence

               gi|91196436     AM205158 RZPD                  2
                               MPMGp1172 Paracentotus
                               lividus MBSB
                               Paracentotus lividus
                               cD.NA clone
                               MPMGp1172PI860Q 5',
                               mRNA sequence

               gi|89437392     AM188140 RZPD                  4
                               MPMGp1172 Paracentrous
                               lividus MBSB
                               Paracentrous lividus
                               cDNA clone
                               MPMGp1172D171Q 5',
                               mRNA sequence

               gi|109399528    133334291 Sea Urchin           4
                               Lantern cDNA library
                               .Strongylocentrouce
                               purpuratus cDNA clone
                               LSP-2B1 5', mRNA
                               sequence

               gi|139265663    AM570670 RZPD                  3
                               MPMGp1173 Paracentrous
                               lividus GASPI.U
                               Paracentrous lividus
                               cDNA clone
                               MPMGp1173O02118Q 5',
                               mRNA sequence

               gi|139245731    AM557988 RZPD                  2
                               MPMGp1172 Paracentrous
                               lividus MBSB
                               Paracentrous lividus
                               cDNA clone
                               MPMGp1172M1164Q 5',
                               mRNA sequence

               gi|139268548    AM551774 RZPD                  2
                               MPMGp1172 Paracentrous
                               lividus MBSB
                               Paracentrous lividus
                               cDNA clone
                               MPMGp1172PI7105Q 5',
                               mRNA sequence

               gi|139302769    AM539511 RZPD                  2
                               MPMGp1171 Paracentrous
                               lividus CLEB
                               Paracentrous lividus
                               cDNA clone
                               MPMGp1171E03207Q 5',
                               mRNA sequence

               gi|89446177     AM212199 RZPDMPMGpll74         1
                               Paracentrous lividus
                               EGG Paracentrous
                               lividus cDNA clone
                               MPMGp1174G0224Q 5',
                               mRNA sequence

               gi|34746608     StrPu691.006926 Sea            1
                               urchin larva cDNA
                               library MPMCp69l
                               Strongylocentrouce
                               purpuratus cDNA clone
                               MPMGp69101599; MPI
                               SURUDI_99015 5', mRNA
                               sequence

               gi|89440162     AM197614 RZPD                  1
                               MPMGp1172 Paracentrous
                               lividus MBSB
                               Paracentrous lividus
                               cDNA clone
                               MPMGp1172J1442Q 5',
                               mRNA sequence

               gi|139242372    AM545798 RZPD                  2
                               MPMGp1171 Paracentrous
                               lividus CLEB
                               Paracentrous lividus
                               cDNA clone
                               MPMGp1171F22200Q 5',
                               mRNA sequence

               gi|89438467     AM197503 RZPD                  1
                               MPMGp1172 Paracentrous
                               lividus MBSB
                               Paracentrous lividus
                               cDNA clone
                               MPMGp1172HI642Q 5',
                               mRNA sequence

               gi|l39268897    AM543994 RZPD                  2
                               MPMGp1171 Paracentrous
                               lividus CLEB
                               Paracentrous lividus
                               cDNA clone
                               MPMGpll71E13116Q 5',
                               mRNA sequence

               gi|91194567     AM194337 RZPD                  1
                               MPMGp1172 Paracentrous
                               lividus MBSB
                               Paracentrous lividus
                               cDNA clone
                               MPMGp1172L1134Q 5',
                               mRNA sequence

               gi|139255468    AM566312 RZPD                  1
                               MPMGp1173 Paracentrous
                               lividus GASPLU
                               Paracentrous lividus
                               cDNA clone
                               MPMGp1173C14108Q 5',
                               mRNA sequence

               gij 109401942   165424028 Sea Urchin           2
                               Radial Nerve cDNA
                               Library
                               Strongylocentrouce
                               purpuratus cDNA clone
                               RNSP-9D11 5', mRNA
                               sequence

               gi|139313404    AM591951 RZPD                  2
                               MPMGpI173 Paracentrous
                               lividus GASPU.
                               Paracentrous lividus
                               cDNA clone
                               MPMGp1173E1357Q 5',
                               mRNA sequence

               gi|139357079    AM52S425 RZPD MPMGpl           1
                               171 Paracentrous
                               lividus CLEB
                               Paracentrous lividus
                               cDNA clone
                               MPMGp1171.107203Q 5',
                               mRNA sequence

               gi|34745864     SirPuMI.006182 Sea             1
                               urchin larva cDNA
                               library MPMGp691
                               Strongylocentrouce
                               purpuratus cDNA clone
                               MPMGp691L1867;
                               MPI_SURUDI_67L18 5',
                               mRNA sequence

               gi|34757996     StrPu621.003311 Sea            3
                               urchin unfertilised
                               egg cDNA Library
                               MPMGpo21
                               Strongylocentrouce
                               purpuratus cDNA clone
                               MPMGp621K2014;
                               MPI_621_14K20 5',
                               mRNA

               gi|139269763    AM527561 RZPD                  1
                               MPMGpll7l Paracentrous
                               lividus CLEB
                               Paracentrous lividus
                               cDNA clone
                               MPMGp117G05205Q 5',
                               mRNA sequence

               gi|57947733     ydf02cO3.yl Sea urchin         1
                               EST Lib 1
                               Sironevlocentrotu
                               purpural us cDNA clone
                               ydf02c03 5', mRNA
                               sequence

               gi|34748064     SlrPu691.008382 Sea            2
                               urchin larva eDNA
                               library MPMGp69 1
                               Strongylocentrouce
                               purpuratus cDNA clone
                               MPMGp691K04142:
                               MPI_SURUDI_142K4 5',
                               mRNA sequence

               gi|139249974    AM560126 RZPD                  1
                               MPMGp1172
                               Paracentrotus lividus
                               MBSB Paracentrotus
                               lividus. cDNA clone
                               MPMGp1172U870Q 5',
                               mRNA sequence

               gi|138478028    AM504745 RZPD                  2
                               MPMGp1171 Paracentrous
                               lividus CLEB
                               Paracentrous lividus
                               cDNA clone
                               MPMGp1171G242Q 5',
                               mRNA sequence

               gi|89442847     AM211931 RZPD                  1
                               MPMGp1174 Paracentrous
                               lividus KGG
                               Paracentrous lividus
                               cDNA clone
                               MPMGp1174J1323Q 5',
                               mRNA sequence

               gi|139279753    AM587839 RZPD                  2
                               MPMGp1173 Paracentrous
                               lividus GASPL.U
                               Paracentrous lividus
                               cDNA clone
                               MPMGp1173D1547Q 5',
                               mRNA sequence

               gi|139251073    AM568303 RZPD MPMGpl           2
                               173 Paracentrous
                               lividus GASPLU
                               Paracentrous lividus
                               cDNA clone
                               MPMGp11731114112Q 5',
                               mRNA sequence

               gi|5713390      MPMGp621_035K08                1
                               MPMGp621
                               Strongylocentrouce
                               purpuratus cDNA done
                               035KOK similar to
                               alpha-tubulin isotype
                               2. mRNA sequence

               gi|61136150     94026114 Sea Urchin            1
                               primary mesenchyme
                               cell cDNA library
                               Strongylocentrouce
                               purpuratus cDNA clone
                               PMCSPR2-149L21 5',
                               mRNA sequence

               gi|l39254559    AM546I96 RZPD                  1
                               MPMGp1171 Paracentrous
                               lividus CLEB
                               Paracentrous lividus
                               cDNA done
                               MPMGp1171J06214Q 5',
                               mRNA sequence

               gi|139345636    AM542633 RZPD                  1

                               MPMGp1171 Paracentrous
                               lividus CLEB
                               Paracentrous lividus
                               cDNA clone
                               MPMGpl17IL17I09Q 5',
                               mRNA sequence

               gi|34751766     StrPu691.009QS4 Sea            1
                               urchin larva cDNA
                               library MPMGp691
                               Strongylocentrouce
                               purpuratus cDNA clone
                               MPMGp691H4106:
                               MPI_SURUDI_106F14 5',
                               mRNA sequence

               gi|109396781    132763544 Sea Urchin           2
                               Mesentary cDNA library
                               Strongylocentrouce
                               purpuratus cDNA clone
                               M.SP-1N8 5', mRNA
                               sequence

               gi|139253277    AM56264I RZPD MPMGpl           2
                               173 Paracentrous
                               lividus GASPLU
                               Paracentrous lividus
                               cDNA clone
                               MPMGp1173F112Q 5',
                               mRNA sequence

               gi|l.39294700   AM579100 RZPD                  1
                               MPMGp1173 Paracentrous
                               lividus s GASPLU
                               Paracentrous lividus
                               cDNA clone
                               MPMGp1173.I2325Q 5',
                               mRNA sequence

               gi|61232318     92891311 Sea Urchin            3
                               primary mesenchyme
                               cell cDNA librarv
                               Strongylocentrouce
                               purpuratus cDNA clone
                               PMCSPR2-73K21 5', mRNA
                               sequence

               gi|62377095     90959498 Sea Urchin            3
                               primary mesenchyme
                               cell cDNA librarv
                               Strongylocentrouce
                               purpuratus cDNA clone
                               PMCSPR2-98N6 5', mRNA
                               sequence

               gi|62383655     92283899 Sea Urchin            2
                               primary mesenchyme
                               cell cDNA librarv
                               Strongylocentrouce
                               purpuratus cDNA clone
                               PMCSPR2-164M9 5', mRNA
                               sequence

               gi|109399379    133201928 Sea Urchin           2
                               Lantern cDNA library
                               Strongylocentrouce
                               purpuratus cDNA clone
                               LSP-1B23 5', mRNA
                               sequence

Uniprot_seaur  tr|Q6WQT5|      Toposome OS =                 60
chin           Q6WQT5_PARU     Paracentrous lividus

               tr|Q3YL94|      Major yolk protein OS         21
               Q3YL94_STRNU    = Strongylocentrouce
                               purpuratus

               tr|Q2637S]      CyI actin OS =                17
               Q26378_TRIGR    Tripneustes gratilla

               tr|O18554|      Cytoplasmic actin CyII        15
               O18554_HELER    (Fragment) OS =
                               Heliocidaris
                               erythrogramma

               sp|P53474|      Actin cytoskeletal 3A         16
               ACTE_STRPU      OS =
                               Strongylocentrouce
                               purpuratus

               tr|O18555|      Cytoplasmic actin CyII        16
               O18555_HELER    (Fragment) OS =
                               Heliocidaris
                               erythrogramma

               tr|Q8WQY0|      Toposome OS =                 12
               Q8WQY0_TRIOR    Tripneustes gratilla

               tr|O18553|      Cytoplasmic actin CyII        12
               O18553_HFLTB    (Fragment) OS =
                               Heliocidaris
                               erythrogramma

               sp|Q07903|      Actin.cytoskeletal 2A         12
               ACTC_STRPU      OS =
                               Strongylocentrouce
                               purpuratus

               tr|Q70JA0|      Nectin OS =                    6
               Q70JA0_PARLI    Paracentrous lividus

               tr|O44344|      Complemenl component           5
               O44344_STRFU    C3 OS =
                               Strongylocentrouce
                               purpuratus

               tr|Q86RA9|      Amassin OS =                   2
               Q86RA9_STRPU    Strongylocentrouce
                               purpuratus

               tr|A9LYL6|      Putative uterine-ovary         1
               A9LYL6_HELTB    speeilie-44 protein OS
                               = Heliocidaris
                               tuberculata

               tr|Q6PTFl|      Fructose- bisphosphatc         1
               Q6PTF1_STRPU    aldolase (Fragment) OS
                               = Strongylocentrouce
                               purpuratus

               tr|D5H3J3|      Ubiquitin OS =                 4
               D5H3J3_P5AMI    Psammechinus miliaris

               sp|P02290|      Histone H2B.1, sperm           1
               H2BS1_PARAN     OS = Parechinus
                               angulosus

               tr|Q6TS36|      Neural alfa2 tubulin           2
               Q6TS36_PARLI    OS = Paracentrous
                               lividus

               tr|O61230|      G-cadherin OS =                1
               O61230_LYTVA    Lytechinus variegatus

               tr|Q27753|      Histone 114 (Fragment)         1
               Q27753_PSAM1    OS = Psammechinus
                               miliaris

               tr|Q68BMl|      Outer arm dynein               1
               Q68BMLANTCR     binding protein OS =
                               Anthocidaris

               sp|Q05634|      Fascin OS =                    2
               FASC_STRPU      Strongylocentrouce
                               purpuratus

               lr|Q8MPK6|      Beta- thymosin OS -            1
               QKMPK6 PARLI    Paracentrous lividus

               tr|Q963T2|      Early historic H3              1
               Q963T2_9ECHN    (Fragment) OS =
                               Tetrapygus mger

               Lr|B6DS92|      Fyn-related kinase OS          1
               B6DS92_STRPU    = Strongylocentrouce
                               purpuratus

               trlQSMXBO|      DNA- binding protein           1
               Q8MXB0_9HCHN    HOX10 (Fragment) OS =
                               Holopneustes
                               purpurescens

               trlQ25427|      USAI OS=Lytechinus             1
               Q25427_LYTVA    variegatus

               sp|Q27802|      Cytoplasmic dynein 2           2
               DYHC2_TRiGR     heavy chain 1 OS =
                               Tripneustes gratilla

               tr|Q6IUG6|      Axin OS - Lytechinus           2
               Q6IUG6_LYTVA    variegaius

Database       Accession      Protein name         Description
               number

EST_seaurchin  gi|50872995    Sp1170               actin related
                              Bacterially
                              activated            protein I
                              coelomocyle.         [Strongylocentrouce
                              arrayed              purpuratus]
                              Strorgvlocetrotus
                              purpuratus cDNA
                              clone Spl170 5',
                              similar to Actin,
                              mKNA
                              sequence

               gi|139309108   AM569285 RZPD        Actin [Ciona
                              MPMGp1173            intestinalis]
                              Paracentrotus
                              lividus CASPLU
                              Paracentrotus
                              lividus cDNA
                              clone
                              MPMGp1173C12115Q
                              5', mRNA sequence

               gi|109400265   132678021 Sea        major yolk protein
                              Urchin
                              Mesentary cDNA       precursor
                              library.             [Strongylocentrouce
                              Strongylocentrouce   purpuratus]

                              purpuratus cDNA
                              done MSP-2L19
                              5', mRNA sequence

               gi|24901528    RN.V/8T3 Sea         actin
                              urchin
                              radial nerve         [Strongylocentrouce
                              Lambda
                              Zap cDNA library     franciscanus]
                              Heliocidaris
                              ervthrogramma cDNA
                              5', similar to Cyl
                              actin,
                              mRNA

               gi|5713384     MPMGp621_0341119     non-muscle actin II
                              MPMGp621             [Hydractinia
                              Strongylocentrouce   echinata]
                              purpuratus to
                              cDNA
                              done 034H19 similar
                              to CyIIIa. mRNA
                              sequence

               gi|89438622    AM2(MW54 RZPD        complement component
                              MPMGp1172            C3
                              Paracentrotus        [Strongylocentrouce
                              lividus              purpuratus]
                              MBSB Paracentrotus
                              lividus cDNA done
                              MPMGp1172J2350Q
                              5', mRNA

               gi|139330259   AM573430 RZPD        similar to cellular
                              MPMGp1173            retinol-binding
                              Paracentrotus        protein type 1b
                              lividus
                              GASPLU               [Strongylocentrouce
                              Paracentrotus
                              lividus cDNA clone   purpuratus]
                              MPMGp1173F08126Q
                              5', mRNA

               gi|34756695    StrPu691.005010      major yolk protei
                              Sea                  precursor
                              urchin larva cDNA    [Strongylocentrouce
                              library MPMGp691     purpuratus]
                              Strangylocentrotus
                              purpuratus cDNA
                              clone
                              MPMGp69101838;
                              MPI_SURUDI38018
                              5', mRNA sequence

               gi|89445358    AM2IU987 RZPD        similar to scavenger
                              MPMGp1172            receptor
                              Paracentrotus        cysteine-rich
                              lividus              protein type 12
                              MBSB Paracenlrotus   precursor, partial
                              liudus cD.NA Clone   [Strongylocentrouce
                              MPMGp1172I0660Q     purpuratus]
                              5', mRNA

               gi|89442983    AM2OH097 RZPD        similar to cathepsin
                              MPMGpl 174           D1. oarlial
                              Paracentrotus        [Strongylocentrouce
                              lividus EGG          purpuratus]
                              Paracentotus
                              lividus cDNA clone
                              MPMGp1174D2214Q
                              5', mRNA

               gi|139328625   AM563391 RZPD        similar to
                              MPMGp1173            transmembrane
                              Paracentrotus        protein UO-44D
                              lividus              [Strongylocentratus
                              GASPLU               purpuralus]
                              Puracentratus
                              lividus cDNA clone
                              MPMGp1173E09100Q
                              5', mRNA

               gi|109399080   133334354 Sea        actin, cytoskeletal
                              Urchin               2B
                              Lantern cDNA         [Strongylocentrouce
                              library              purpuratus]
                              Strangylocentrotus
                              purpuratus cDNA
                              clone
                              LSP-2F21 5', mRNA
                              sequence

               gi|62385757    92292867 Sea         Actin-15A
                              Urchin               [Strongylocentrouce
                              primary mesenchyme   purpuratus]
                              cell cDNA library
                              Strangylocentrotus
                              purpuralus cDNA
                              clone
                              PMCSPR2-165P21
                              5', mRNA

               gi|89457047    AM214690 RZPD        similar to
                              MPMGp1174            tetraspanin. partial
                              Paracentotus         [Strongylocentrouce
                              lividus HGG          purpuratus]
                              Paracentotus
                              lividus cDNA
                              clone
                              MPMGp1174E1230Q
                              5', mRNA sequence

               gi|91196436    AM205158 RZPD        cellular
                              MPMGp1172            retinol-binding
                              Paracentotus         protein type 1b
                              lividus              [Strongylocentrouce
                              MBSB Paracentotus    purpuratus]
                              lividus cD.NA clone
                              MPMGp1172PI860Q
                              5', mRNA sequence

               gi|89437392    AM188140 RZPD        similar to alpha
                              MPMGp1172            macroglobulin
                              Paracentrous         [Strongylocentrouce
                              lividus              purpuratus]
                              MBSB Paracentrous
                              lividus cDNA clone
                              MPMGp1172D171Q
                              5', mRNA
                              sequence

               gi|109399528   133334291 Sea        major yolk protein
                              Urchin               precursor
                              Lantern cDNA         [Strongylocentrouce
                              library              purpuratus]
                              Strongylocentrouce
                              purpuratus cDNA
                              clone LSP-2B1 5',
                              mRNA sequence

               gi|139265663   AM570670 RZPD        nectin precursor
                              MPMGp1173            [Paracentrous
                              Paracentrous         lividus]
                              lividus
                              GASPI.U
                              Paracentrous
                              lividus
                              cDNA clone
                              MPMGp1173O02118Q
                              5', mRNA sequence

               gi|139245731   AM557988 RZPD        similar to
                              MPMGp1172            apolipoprotein B
                              Paracentrous         [Strongylocentrouce
                              lividus              purpuratus]
                              MBSB
                              Paracentrous
                              lividus cDNA
                              clone
                              MPMGp1172M1164Q
                              5', mRNA sequence

               gi|139268548   AM551774 RZPD        hypothetical protein
                              MPMGp1172            [Strongylocentrouce
                              Paracentrous         purpuratus]
                              lividus
                              MBSB
                              Paracentrous
                              lividus cDNA
                              clone
                              MPMGp1172PI7105Q
                              5', mRNA sequence

               gi|139302769   AM539511 RZPD        propralein
                              MPMGp1171            convertase
                              Paracentrous         subtilisin/kexin
                              lividus              type
                              CLEB                 9 preproprotein
                              Paracentrous         [Strongylocentrouce
                              lividus cDNA         purpuratus]
                              clone
                              MPMGp1171E03207Q
                              5', mRNA sequence

               gi|89446177    AM212199             similar to
                                                   G-cadherin
                              RZPDMPMGpll74        [Strongylocentrouce
                              Paracentrous         purpuratus]
                              lividus
                              EGG Paracentrous
                              lividus cDNA
                              clone
                              MPMGp1174G0224Q
                              5'mRNA sequence

               gi|34746608    StrPu691.006926      similar to catalase
                              Sea                  [Strongylocentrouce
                              urchin larva cDNA    purpuratus]
                              library MPMCp69l
                              Strongylocentrouce
                              purpuratus cDNA
                              clone
                              MPMGp69101599;
                              MPI
                              SURUDI_99015
                              5', mRNA sequence

               gi|89440162    AM197614 RZPD        Profilin
                              MPMGp1172
                              Paracentrous
                              lividus MBSB
                              Paracentrous
                              lividus
                              cDNA clone
                              MPMGp1172J1442Q
                              5'mRNA sequence

               gi|139242372   AM545798 RZPD        similar to
                              MPMGp1171            Glutathione
                              Paracentrous         s-transferase
                              lividus              protein 35
                              CLEB Paracentrous    [Strongylocentrouce
                              lividus cDNA clone   purpuratus]
                              MPMGp1171F22200Q
                              5', mRNA sequence

               gi|89438467    AM197503 RZPD        tropomyosin
                              MPMGp1172            [Strongylocentrouce
                              Paracentrous         purpuratus]
                              lividus
                              MBSB Paracentrous
                              lividus cDNA clone
                              MPMGp1172HI642Q
                              5', mRNA sequence

               gi|l39268897   AM543994 RZPD        hypothetical protein
                              MPMGp1171            [Strongylocentrouce
                              Paracentrous         purpuratus]
                              lividus
                              CLEB Paracentrous
                              lividus cDNA clone
                              MPMGpll71E13116Q
                              5', mRNA sequence

               gi|91194567    AM194337 RZPD        similar to
                              MPMGp1172            prominin-1.sl
                              Paracentrous         splice
                              lividus              variant, partial
                              MBSB Paracentrous    [Strongylocentrouce
                              lividus cDNA clone   purpuratus]
                              MPMGp1172L1134Q
                              5', mRNA sequence

               gi|139255468   AM566312 RZPD        similar to
                              MPMGp1173            glutathione-
                              Paracentrous         S-transferase
                              lividus              [Strongylocentrouce
                              GASPLU                purpuratus]
                              Paracentrous
                              lividus
                              cDNA clone
                              MPMGp1173C14108Q
                              5', mRNA sequence

               gi|109401942   165424028 Sea        similar to
                              Urchin               apolipophorin
                              Radial Nerve cDNA    precursor protein
                              Library              [Strongylocentrouce
                              Strongylocentrouce   purpuratus]
                              purpuratus cDNA
                              clone
                              RNSP-9D11 5', mRNA
                              sequence

               gi|139313404   AM591951 RZPD        similar to
                              MPMGpI173            14-3-3-like
                              Paracentrous         protein 2
                              lividus              [Strongyloeeniroius
                              GASPU.Paracentrous   purpuratus]
                              lividus cDNA clone
                              MPMGp1173E1357Q
                              5', mRNA sequence

               gi|139357079   AM52S425 RZPD        hypothetical protein
                              MPMGpl 171           [Strongylocentrouce
                              Paracentrous         purpuratus]
                              lividus
                              CLEB Paracentrous
                              lividus cDNA clone
                              MPMGp1171.107203Q
                              5', mRNA sequence

               gi|34745864    SirPuMI.006182 Sea   similar to
                              urchin larva cDNA    arylsulfatase
                              library MPMGp691     isoform 1
                              Strongylocentrouce   [Strongylocentrouce
                              purpuratus cDNA      purpuratus]
                              clone
                              MPMGp691L1867;
                              MPI_SURUDI_67L18
                              5', mRNA sequence

               gi|34757996    StrPu621.003311      ubiquitin A-52
                              Sea urchin           residue
                              unfertilised         ribosomal protein
                              egg cDNA Library     lusion product
                              MPMGpo21             1 [Homo
                              Strongylocentrouce   sapiens]
                              purpuratus cDNA
                              clone
                              MPMGp621K2014;
                              MPI_621_14K20 5',
                              mRNA

               gi|139269763   AM527561 RZPD        similar to
                              MPMGpll7l            snEchinoidin
                              Paracentrous         [Strongylocentrouce
                              lividus              purpuratus]
                              CLEB Paracentrous
                              lividus cDNA clone
                              MPMGp117G05205Q
                              5', mRNA sequence

               gi|57947733    ydf02cO3.yl Sea      hypothetical
                              urchin EST Lib 1     protein
                              Sironevlocentrotu    [Strongylocentrouce
                              purpural us cDNA     purpuratus]
                              clone ydf02c03
                              5', mRNA
                              sequence

               gi|34748064    SlrPu691.008382      Annexin A4
                              Sea urchin larva     [Strongylocentrouce
                              eDNA library         purpuratus]
                              MPMGp691
                              Strongylocentrouce
                              purpuratus cDNA
                              clone
                              MPMGp691K04142:
                              MPI_SURUDI_142K4
                              5', mRNA sequence

               gi|139249974   AM560126 RZPD        similar to
                              MPMGp1172            zonadhesin
                              Paracentrotus        [Strongylocentrouce
                              lividus              purpuratus]
                              MBSB
                              Paracentrotus
                              lividus. cDNA
                              clone
                              MPMGp1172U870Q
                              5', mRNA
                              sequence

               gi|138478028   AM504745 RZPD        similar to H4
                              MPMGp1171            histone
                              Paracentrous         protein
                              lividus              [Strongylocentrouce
                              CLEB                 purpuratus]
                              Paracentrous
                              lividus cDNA
                              clone
                              MPMGp1171G242Q
                              5', mRNA
                              sequence

               gi|89442847    AM211931 RZPD        Glutathione
                              MPMGp1174            s-transferase
                              Paracentrous         protein 35
                              lividus              [Strongylocentrouce
                              KGG                  purpuratus]
                              Paracentrous
                              lividus cDNA
                              clone
                              MPMGp1174J1323Q
                              5', mRNA
                              sequence

               gi|139279753   AM587839 RZPD        similar to
                              MPMGp1173            membrane alanine
                              Paracentrous         aminopeptidase
                              lividus GASPL.U      precursor variant
                              Paracentrous         [Strongylocentrouce
                              lividus              purpuratus]
                              cDNA clone
                              MPMGp1173D1547Q
                              5', mRNA sequence

               gi|139251073   AM568303 RZPD        hypothetical
                              MPMGpl               protein
                              173 Paracentrous     [Strongylocentrouce
                              lividus GASPLU       purpuratus]
                              Paracentrous
                              lividus
                              cDNA clone
                              MPMGp11731114112Q
                              5', mRNA sequence

               gi|5713390     MPMGp621_035K08      Beta-tubulin
                              MPMGp621             [Paracentrous
                              Strongylocentrouce   lividus]
                              purpuratus cDNA
                              done 035KOK
                              similar to
                              alpha-tubulin
                              isotype 5', mRNA
                              sequence

               gi|61136150    94026114 Sea         Wnt8
                              Urchin               [Hemicentrotus
                              primary mesenchyme   pulcherrimus]
                              cell cDNA library
                              Strongylocentrouce
                              purpuratus cDNA
                              clone
                              PMCSPR2-149L21
                              5', mRNA sequence

               gi|l39254559   AM546I96 RZPD        calmodulin 2
                              MPMGp1171            [Strongylocentrouce
                              Paracentrous         purpuratus]
                              lividus CLEB
                              Paracentrous
                              lividus
                              cDNA done
                              MPMGp1171J06214Q
                              5', mRNA sequence

               gi|139345636   AM542633 RZPD        alpha
                              MPMGp1171            macroglobulin
                              Paracentrous         [Strongylocentrouce
                              lividus CLEB         purpuratus]
                              Paracentrous
                              lividus
                              cDNA clone
                              MPMGpl17IL17I09Q
                              5', mRNA sequence

               gi|34751766    StrPu691.009QS4      DNA
                              Sea                  topoisomerasell
                              urchin larva cDNA    _beta
                              library MPMGp691     [Strongylocentrouce
                              Strongylocentrouce   purpuratus]
                              purpuratus cDNA
                              clone
                              MPMGp691H4106:
                              MPI_SURUDI_106F14
                              5', mRNA sequence

               gi|109396781   132763544 Sea        major yolk protein
                              Urchin               precursor
                              Mesentary cDNA       [Strongylocentrouce
                              library              purpuratus]
                              Strongylocentrouce
                              purpuratus cDNA
                              clone M.SP-1N8
                              5', mRNA sequence

               gi|139253277   AM56264I RZPD        hypothetical
                              MPMGpl               protein
                              173 Paracentrous     [Strongylocentrouce
                              lividus GASPLU       purpuratus]
                              Paracentrous
                              lividus
                              cDNA clone
                              MPMGp1173F112Q
                              5', mRNA sequence

               gi|l.39294700  AM579100 RZPD        Rap 1b-prov
                              MPMGp1173            protein
                              Paracentrous         [Strongylocentrouce
                              lividus s GASPLU     purpuratus]
                              Paracentrous
                              lividus
                              cDNA clone
                              MPMGp1173.I2325Q
                              5', mRNA sequence

               gi|61232318    92891311 Sea         polyubiquitin-
                              Urchin               C-like
                              primary mesenchyme   [Susscrofa]
                              cell cDNA librarv
                              Strongylocentrouce
                              purpuratus cDNA
                              clone
                              PMCSPR2-73K21
                              5', mRNA sequence

               gi|62377095    90959498 Sea         polyubiquhin
                              Urchin               [Tribolium
                              primary mesenchyme   castaneum]
                              cell cDNA librarv
                              Strongylocentrouce
                              purpuratus cDNA
                              clone
                              PMCSPR2-98N6
                              5', mRNA sequence

               gi|62383655    92283899 Sea         ubiquitin
                              Urchin               [Monodelphis
                              primary mesenchyme   domestica]
                              cell cDNA librarv
                              Strongylocentrouce
                              purpuratus cDNA
                              clone
                              PMCSPR2-164M9
                              5', mRNA sequence

               gi|109399379   133201928 Sea        Actin,
                              Urchin               eytoskeletal
                              Lantern cDNA         [Heliocidaris
                              library              tuberculata]
                              Strongylocentrouce
                              purpuratus cDNA
                              clone
                              LSP-1B23 5', mRNA
                              sequence

Uniprot_seaur  tr|Q6WQT5|     Toposome OS =
chin           Q6WQT5_PARU    Paracentrous
                              lividus

               tr|Q3YL94|     Major yolk protein
               Q3YL94_STRNU   OS =
                              Strongylocentrouce
                              purpuratus

               tr|Q2637S]     CyI actin OS =
               Q26378_TRIGR   Tripneustes
                              gratilla

               tr|O18554|     Cytoplasmic actin
               O18554_HELER   CyII (Fragment)
                              OS =
                              Heliocidaris
                              erythrogramma

               sp|P53474|     Actin.cytoskeletal
               ACTE_STRPU     3A OS =
                              Strongylocentrouce
                              purpuratus

               tr|O18555|     Cytoplasmic actin
               O18555_HELER   CyII (Fragment)
                              OS =
                              Heliocidaris
                              erythrogramma

               tr|Q8WQY0|     Toposome OS =
               Q8WQY0_TRIOR   Tripneustes
                              gratilla

               tr|O18553|     Cytoplasmic actin
               O18553_HFLTB   CyII (Fragment)
                              OS =
                              Heliocidaris
                              erythrogramma

               sp|Q07903|     Actin.cytoskeletal
               ACTC_STRPU     2A OS =
                              Strongylocentrouce
                              purpuratus

               tr|Q70JA0|     Nectin OS =
               Q70JA0_PARLI   Paracentrous
                              lividus

               tr|O44344|     Complemenl
               O44344_STRFU   component C3 OS =
                              Strongylocentrouce
                              purpuratus

               tr|Q86RA9|     Amassin OS =
               Q86RA9_STRPU   Strongylocentrouce
                              purpuratus

               tr|A9LYL6|     Putative
               A9LYL6_HELTB   uterine-ovary
                              speeilie-44 protein
                              OS = Heliocidaris
                              tuberculata

               tr|Q6PTFl|     Fructose-
               Q6PTF1_STRPU   bisphosphatc
                              aldolase (Fragment)
                              OS =
                              Strongylocentrouce
                              purpuratus

               tr|D5H3J3|     Ubiquitin OS =
               D5H3J3_P5AMI   Psammechinus
                              miliaris

               sp|P02290|     Histone H2B.1,
               H2BS1_PARAN    sperm OS =
                              Parechinus
                              angulosus

               tr|Q6TS36|     Neural alfa2
               Q6TS36_PARLI   tubulin OS =
                              Paracentrous
                              lividus

               tr|O61230|     G-cadherin OS
               O61230_LYTVA   = Lytechinus
                              variegatus

               tr|Q27753|     Histone 114
               Q27753_PSAM1   (Fragment) OS =
                              Psammechinus
                              miliaris

               tr|Q68BMl|     Outer arm dynein
               Q68BMLANTCR    binding protein
                              OS
                              =Anthocidaris

               sp|Q05634|     Fascin OS =
               FASC_STRPU     Strongylocentrouce
                              purpuratus

               lr|Q8MPK6|     Beta- thymosin OS -
               QKMPK6 PARLI   Paracentrous
                              lividus

               tr|Q963T2|     Early historic H3
               Q963T2_9ECHN   (Fragment) OS =
                              Tetrapygus mger

               Lr|B6DS92|     Fyn-related kinase
               B6DS92_STRPU   OS =
                              Strongylocentrouce
                              purpuratus

               trlQSMXBO|     DNA-binding protein
               Q8MXB0_9HCHN   HOX10 (Fragment)
                              OS = Holopneustes
                              purpurescens

               trlQ25427|     USAI OS=
               Q25427_LYTVA   Lytechinus
                              variegatus

               sp|Q27802|     Cytoplasmic dynein
               DYHC2_TRiGR    2 heavy chain 1
                              OS = Tripneustes
                              gratilla

               tr|Q6IUG6|     Axin OS-Lytechinus
               Q6IUG6_LYTVA   variegaius

Database       Accession       Protein name             Max  total
               number                                        score

EST_seaurchin  gi|50872995     Sp1170 Bacterially       605    661
                               activated coelomocyle.
                               arrayed
                               Strorgvlocetrotus
                               purpuratus cDNA clone
                               Spl170 5', similar to
                               Actin, mKNA sequence

               gi|139309108    AM569285 RZPD            560    560
                               MPMGp1173
                               Paracentrotus lividus
                               CASPLU Paracentrotus
                               lividus cDNA clone
                               MPMGp1173CI2I I5Q 5',
                               mRNA sequence

               gi|109400265    132678021 Sea Urchin     632    632
                               Mesentary cDNA library
                               Strongylocentrouce
                               purpuratus cDNA done
                               MSP-2L19 5', mRNA
                               sequence

               gi|24901528     RN.V/8T3 Sea urchin      406    550
                               radial nerve Lambda
                               Zap cDNA library
                               Heliocidaris
                               ervthrogramma cDNA 5',
                               similar to Cyl actin,
                               mRNA

               gi|5713384      MPMGp621_0341119         278    278
                               MPMGp621
                               Strongylocentrouce
                               purpuratus to s cDNA
                               done 034H19 similar to
                               CyIIIa. mRNA sequence

               gi|89438622     AM2(MW54 RZPD            182    182
                               MPMGp1172
                               Paracentrotus lividus
                               MBSB Paracentrotus
                               lividus cDNA done
                               MPMGp1172J2350Q 5',
                               mRNA

               gi|139330259    AM573430 RZPD            187    187
                               MPMGp1173
                               Paracentrotus lividus
                               GASPLU Paracentrotus
                               lividus cDNA clone
                               MPMGp1173F08126Q 5',
                               mRNA

               gi|34756695     StrPu69 1.005010 Sea     365    420
                               urchin larva cDNA
                               library MPMGp691
                               Strangylocentrotus
                               purpuratus cDNA clone
                               MPMGp69101838;
                               MPI_SURUDI 38018 5',
                               mRNA sequence

               gi|89445358     AM2IU987 RZPD            222   1786
                               MPMGp1172
                               Paracentrotus lividus
                               MBSB Paracenlrotus
                               liudus cD.NA Clone
                               MPMGp1172I0660Q 5',
                               mRNA

               gi|89442983     AM2OH097 RZPD MPMGpl     332    332
                               174 Paracentrotus
                               lividus EGG
                               Paracentotus lividus
                               cDNA clone
                               MPMGp1174D2214Q 5'.
                               mRNA

               gi|139328625    AM563391 RZPD            445    445
                               MPMGp1173
                               Paracentrotus lividus
                               GASPLU Puracentratus
                               lividus cDNA clone
                               MPMGp1173E09100Q 5',
                               mRNA

               gi|109399080    133334354 Sea Urchin     545    545
                               Lantern cDNA library
                               Strangylocentrotus
                               purpuratus cDNA clone
                               LSP-2F21 5', mRNA
                               sequence

               gi|62385757     92292867 Sea Urchin      508    508
                               primary mesenchyme
                               cell cDNA J i bran
                               Strangylocentrotus
                               purpuralus cDNA clone
                               PMCSPR2-165P21 5',
                               mRNA

               gi|89457047     AM214690 RZPD            145    145
                               MPMGp1174 Paracentotus
                               lividus HGG
                               Paracentotus lividus
                               cDNA clone
                               MPMGp1174E1230Q 5',
                               mRNA sequence

               gi|91196436     AM205158 RZPD            187    187
                               MPMGp1172 Paracentotus
                               lividus MBSB
                               Paracentotus lividus
                               cD.NA clone
                               MPMGp1172PI860Q 5',
                               mRNA sequence

               gi|89437392     AM188140 RZPD            270    270
                               MPMGp1172 Paracentrous
                               lividus MBSB
                               Paracentrous lividus
                               cDNA clone
                               MPMGp1172D171Q 5',
                               mRNA sequence

               gi|109399528    133334291 Sea Urchin     379    526
                               Lantern cDNA library
                               .Strongylocentrouce
                               purpuratus cDNA clone
                               LSP-2B1 5', mRNA
                               sequence

               gi|139265663    AM570670 RZPD            557    557
                               MPMGp1173 Paracentrous
                               lividus GASPI.U
                               Paracentrous lividus
                               cDNA clone
                               MPMGp1173O02118Q 5',
                               mRNA sequence

               gi|139245731    AM557988 RZPD            395    444
                               MPMGp1172 Paracentrous
                               lividus MBSB
                               Paracentrous lividus
                               cDNA clone
                               MPMGp1172M1164Q 5',
                               mRNA sequence

               gi|139268548    AM551774 RZPD            225    225
                               MPMGp1172 Paracentrous
                               lividus MBSB
                               Paracentrous lividus
                               cDNA clone
                               MPMGp1172PI7105Q 5',
                               mRNA sequence

               gi|139302769    AM539511 RZPD            342    342
                               MPMGp1171 Paracentrous
                               lividus CLEB
                               Paracentrous lividus
                               cDNA clone
                               MPMGp1171E03207Q 5',
                               mRNA sequence

               gi|89446177     AM212199 RZPDMPMGpll74   281    332
                               Paracentrous lividus
                               EGG Paracentrous
                               lividus cDNA clone
                               MPMGp1174G0224Q 5'
                               mRNA sequence

               gi|34746608     StrPu691.006926 Sea      387    387
                               urchin larva cDNA
                               library MPMCp69l
                               Strongylocentrouce
                               purpuratus cDNA clone
                               MPMGp69101599; MPI
                               SURUDI_99015 5', mRNA
                               sequence

               gi|89440162     AM197614 RZPD            214    214
                               MPMGp1172 Paracentrous
                               lividus MBSB
                               Paracentrous lividus
                               cDNA clone
                               MPMGp1172J1442Q 5'
                               mRNA sequence

               gi|139242372    AM545798 RZPD            210    210
                               MPMGp1171 Paracentrous
                               lividus CLEB
                               Paracentrous lividus
                               cDNA clone
                               MPMGp1171F22200Q 5'
                               mRNA sequence

               gi|89438467     AM197503 RZPD            352    352
                               MPMGp1172 Paracentrous
                               lividus MBSB
                               Paracentrous lividus
                               cDNA clone
                               MPMGp1172HI642Q 5',
                               mRNA sequence

               gi|l39268897    AM543994 RZPD            553    553
                               MPMGp1171 Paracentrous
                               lividus CLEB
                               Paracentrous lividus
                               cDNA clone
                               MPMGpll71E13116Q 5',
                               mRNA sequence

               gi|91194567     AM194337 RZPD            362    362
                               MPMGp1172 Paracentrous
                               lividus MBSB
                               Paracentrous lividus
                               cDNA clone
                               MPMGp1172L1134Q 5',
                               mRNA sequence

               gi|139255468    AM566312 RZPD            172    172
                               MPMGp1173 Paracentrous
                               lividus GASPLU
                               Paracentrous lividus
                               cDNA clone
                               MPMGp1173C14108Q 5',
                               mRNA sequence

               gi|109401942    165424028 Sea Urchin     559    600
                               Radial Nerve cDNA
                               Library
                               Strongylocentrouce
                               purpuratus cDNA clone
                               RNSP-9D11 5', mRNA
                               sequence

               gi|139313404    AM591951 RZPD            311    311
                               MPMGpI173 Paracentrous
                               lividus GASPU.
                               Paracentrous lividus
                               cDNA clone
                               MPMGp1173E1357Q 5',
                               mRNA sequence

               gi|139357079    AM52S425 RZPD MPMGpl     343    343
                               171 Paracentrous
                               lividus CLEB
                               Paracentrous lividus
                               cDNA clone
                               MPMGp1171.107203Q 5'
                               mRNA sequence

               gi|34745864     SirPuMI.006182 Sea       350    350
                               urchin larva cDNA
                               library MPMGp691
                               Strongylocentrouce
                               purpuratus cDNA clone
                               MPMGp691L1867;
                               MPI_SURUDI_67L18 5'
                               mRNA sequence

               gi|34757996     StrPu621.003311 Sea      266    266
                               urchin unfertilised
                               egg cDNA Library
                               MPMGpo21
                               Strongylocentrouce
                               purpuratus cDNA clone
                               MPMGp621K2014;
                               MPI_621_14K20 5'.
                               mRNA

               gi|139269763    AM527561 RZPD            181    181
                               MPMGpll7l Paracentrous
                               lividus CLEB
                               Paracentrous lividus
                               cDNA clone
                               MPMGp117G05205Q 5'
                               mRNA sequence

               gi|57947733     ydf02cO3.yl Sea urchin  89.7    143
                               EST Lib 1
                               Sironevlocentrotu.-.
                               purpura! us cDNA clone
                               ydf02c03 5'. mRNA
                               sequence

               gi|34748064     SlrPu691.008382 Sea      380    562
                               urchin larva eDNA
                               library MPMGp69 1
                               Strongylocentrouce
                               purpuratus cDNA clone
                               MPMGp691K04142:
                               MPI_SURUDI_142K4 5',
                               mRNA sequence

               gi|139249974    AM560126 RZPD            496    585
                               MPMGp1172
                               Paracentrotus lividus
                               MBSB Paracentrotus
                               lividus. cDNA clone
                               MPMGp1172U870Q 5'.
                               mRNA sequence

               gi|138478028    AM504745 RZPD            164    164
                               MPMGp1171 Paracentrous
                               lividus CLEB
                               Paracentrous lividus
                               cDNA clone
                               MPMGp1171G242Q 5',
                               mRNA sequence

               gi|89442847     AM211931 RZPD            205    205
                               MPMGp1174 Paracentrous
                               lividus KGG
                               Paracentrous lividus
                               cDNA clone
                               MPMGp1174J1323Q 5',
                               mRNA sequence

               gi|139279753    AM587839 RZPD            437    437
                               MPMGp1173 Paracentrous
                               lividus GASPL.U
                               Paracentrous lividus
                               cDNA clone
                               MPMGp1173D1547Q 5',
                               mRNA sequence

               gi|139251073    AM568303 RZPD MPMGpl     284    499
                               173 Paracentrous
                               lividus GASPLU
                               Paracentrous lividus
                               cDNA clone
                               MPMGp11731114112Q 5',
                               mRNA sequence

               gi|5713390      MPMGp621_035K08          511    511
                               MPMGp621
                               Strongylocentrouce
                               purpuratus cDNA done
                               035KOK similar to
                               alpha-tubulin isotype
                               2. mRNA sequence

               gi|61136150     94026114 Sea Urchin      367    367
                               primary mesenchyme
                               cell cDNA library
                               Strongylocentrouce
                               purpuratus cDNA clone
                               PMCSPR2-149L21 5',
                               mRNA sequence

               gi|l39254559    AM546I96 RZPD            293    293
                               MPMGp1171 Paracentrous
                               lividus CLEB
                               Paracentrous lividus
                               cDNA done
                               MPMGp1171J06214Q 5',
                               mRNA sequence

               gi|139345636    AM542633 RZPD            234    234
                               MPMGp1171 Paracentrous
                               lividus CLEB
                               Paracentrous lividus
                               cDNA clone
                               MPMGpl17IL17I09Q 5',
                               mRNA sequence

               gi|34751766     StrPu691.009QS4 Sea      372    372
                               urchin larva cDNA
                               library MPMGp691
                               Strongylocentrouce
                               purpuratus cDNA clone
                               MPMGp691H4106:
                               MPI_SURUDI_106F14 5',
                               mRNA sequence

               gi|109396781    132763544 Sea Urchin     561    615
                               Mesentary cDNA library
                               Strongylocentrouce
                               purpuratus cDNA clone
                               M.SP-1N8 3'. mRNA
                               sequence

               gi|139253277    AM56264I RZPD MPMGpl     263    471
                               173 Paracentrous
                               lividus GASPLU
                               Paracentrous lividus
                               cDNA clone
                               MPMGp1173F112Q 5',.
                               mRNA sequence

               gi|l.39294700   AM579100 RZPD            238    238
                               MPMGp1173 Paracentrous
                               lividus s GASPLU
                               Paracentrous lividus
                               cDNA clone
                               MPMGp1173.I2325Q 5',
                               mRNA sequence

               gi|61232318     92891311 Sea Urchin      265   1714
                               primary mesenchyme
                               cell cDNA librarv
                               Strongylocentrouce
                               purpuratus cDNA clone
                               PMCSPR2-73K21 5', mRNA
                               sequence

               gi|62377095     90959498 Sea Urchin      512   3496
                               primary mesenchyme
                               cell cDNA librarv
                               Strongylocentrouce
                               purpuratus cDNA clone
                               PMCSPR2-98N6 5', mRNA
                               sequence

               gi|62383655     92283899 Sea Urchin      502   3941
                               primary mesenchyme
                               cell cDNA librarv
                               Strongylocentrouce
                               purpuratus cDNA clone
                               PMCSPR2-164M9 5', mRNA
                               sequence

               gi|109399379    133201928 Sea Urchin     647    647
                               Lantern cDNA library
                               Strongylocentrouce
                               purpuratus cDNA clone
                               LSP-1B23 5', mRNA
                               sequence

Uniprot_seaur  tr|Q6WQT5|      Toposome OS =
chin           Q6WQT5_PARU     Paracentrous lividus

               tr|Q3YL94|      Major yolk protein OS
               Q3YL94_STRNU    = Strongylocentrouce
                               purpuratus

               tr|Q2637S]      CyI actin OS =
               Q26378_TRIGR    Tripneustes gratilla

               tr|O18554|      Cytoplasmic actin CyII
               O18554_HELER    (Fragment) OS =
                               Heliocidaris
                               erythrogramma

               sp|P53474|      Actin.cytoskeletal 3A
               ACTE_STRPU      OS =
                               Strongylocentrouce
                               purpuratus

               tr|O18555|      Cytoplasmic actin CyII
               O18555_HELER    (Fragment) OS =
                               Heliocidaris
                               erythrogramma

               tr|Q8WQY0|      Toposome OS =
               Q8WQY0_TRIOR    Tripneustes gratilla

               tr|O18553|      Cytoplasmic actin CyII
               O18553_HFLTB    (Fragment) OS =
                               Heliocidaris
                               erythrogramma

               sp|Q07903|      Actin.cytoskeletal 2A
               ACTC_STRPU      OS =
                               Strongylocentrouce
                               purpuratus

               tr|Q70JA0|      Nectin OS =
               Q70JA0_PARLI    Paracentrous lividus

               tr|O44344|      Complemenl component
               O44344_STRFU    C3 OS =
                               Strongylocentrouce
                               purpuratus

               tr|Q86RA9|      Amassin OS =
               Q86RA9_STRPU    Strongylocentrouce
                               purpuratus

               tr|A9LYL6|      Putative uterine-ovary
               A9LYL6_HELTB    speeilie-44 protein OS
                               = Heliocidaris
                               tuberculata

               tr|Q6PTFl|      Fructose- bisphosphatc
               Q6PTF1_STRPU    aldolase (Fragment) OS
                               = Strongylocentrouce
                               purpuratus

               tr|D5H3J3|      Ubiquitin OS =
               D5H3J3_P5AMI    Psammechinus miliaris

               sp|P02290|      Histone H2B.1, sperm
               H2BS1_PARAN     OS = Parechinus
                               angulosus

               tr|Q6TS36|      Neural alfa2 tubulin
               Q6TS36_PARLI    OS = Paracentrous
                               lividus

               tr|O61230|      G-cadherin OS =
               O61230_LYTVA    Lytechinus variegatus

               tr|Q27753|      Histone 114 (Fragment)
               Q27753_PSAM1    OS = Psammechinus
                               miliaris

               tr|Q68BMl|      Outer arm dynein
               Q68BMLANTCR     binding protein OS =
                               Anthocidaris

               sp|Q05634|      Fascin OS =
               FASC_STRPU      Strongylocentrouce
                               purpuratus

               lr|Q8MPK6|      Beta- thymosin OS -
               QKMPK6 PARLI    Paracentrous lividus

               tr|Q963T2|      Early historic H3
               Q963T2_9ECHN    (Fragment) OS =
                               Tetrapygus mger

               Lr|B6DS92|      Fyn-related kinase OS
               B6DS92_STRPU    = Strongylocentrouce
                               purpuratus

               trlQSMXBO|      DNA- binding protein
               Q8MXB0_9HCHN    HOX10 (Fragment) OS =
                               Holopneustes
                               purpurescens

               trlQ25427|      USAI OS=Lytechinus
               Q25427_LYTVA    variegatus

               sp|Q27802|      Cytoplasmic dynein 2
               DYHC2_TRiGR     heavy chain 1 OS =
                               Tripneustes gratilla

               tr|Q6IUG6|      Axin OS - Lytechinus
               Q6IUG6_LYTVA    variegaius

Database       Accession      Protein name             Query
               number                                 coverage

EST_seaurchin  gi|50872995    Sp1170 Bacterially       76%
                              activated coelomocyle.
                              arrayed
                              Strorgvlocetrotus
                              purpuratus cDNA clone
                              Spl170 5' similar to
                              Actin, mKNA sequence

               gi|139309108   AM569285 RZPD            98%
                              MPMGp1173
                              Paracentrotus lividus
                              CASPLU Paracentrotus
                              lividus cDNA clone
                              MPMGp1173CI2I I5Q 5',
                              mRNA sequence

               gi|109400265   132678021 Sea Urchin     95%
                              Mesentary cDNA library
                              .Strongylocentrouce
                              purpuratus cDNA done
                              MSP-2L19 5', mRNA
                              sequence

               gi|24901528    RN.V/8T3 Sea urchin      69%
                              radial nerve Lambda
                              Zap cDNA library
                              Heliocidaris
                              ervthrogramma cDNA 5',
                              similar to Cyl actin,
                              mRNA

               gi|5713384     MPMGp621_0341119         40%
                              MPMGp621
                              Strongylocentrouce
                              purpuratus to s cDNA
                              done 034H19 similar to
                              CyIIIa. mRNA sequence

               gi|89438622    AM2(MW54 RZPD            98%
                              MPMGp1172
                              Paracentrotus lividus
                              MBSB Paracentrotus
                              lividus cDNA done
                              MPMGp1172J2350Q 5',
                              mRNA

               gi|139330259   AM573430 RZPD            53%
                              MPMGp1173
                              Paracentrotus lividus
                              GASPLU Paracentrotus
                              lividus cDNA clone
                              MPMGp1173F08126Q 5',
                              mRNA

               gi|34756695    StrPu69 1.005010 Sea     95%
                              urchin larva cDNA
                              library MPMGp691
                              Strangylocentrotus
                              purpuratus cDNA clone
                              MPMGp69101838;
                              MPI_SURUDI 38018 5',
                              mRNA sequence

               gi|89445358    AM2IU987 RZPD            80%
                              MPMGp1172
                              Paracentrotus lividus
                              MBSB Paracenlrotus
                              liudus cD.NA Clone
                              MPMGp117210660Q 5',
                              mRNA

               gi|89442983    AM2OH097 RZPD MPMGpl    65'%
                              174 Paracentrotus
                              lividus EGG
                              Paracentotus lividus
                              cDNA clone
                              MPMGp1174D2214Q 5',
                              mRNA

               gi|139328625   AM563391 RZPD            89%
                              MPMGp1173
                              Paracentrotus lividus
                              GASPLU Puracentratus
                              lividus cDNA clone
                              MPMGp1173E09100Q 5',
                              mRNA

               gi|109399080   133334354 Sea Urchin     87%
                              Lantern cDNA library
                              Strangylocentrotus
                              purpuratus cDNA clone
                              LSP-2F21 5', mRNA
                              sequence

               gi|62385757    92292867 Sea Urchin      84%
                              primary mesenchyme
                              cell cDNA J i bran
                              Strangylocentrotus
                              purpuralus cDNA clone
                              PMCSPR2-165P21 5',
                              mRNA

               gi|89457047    AM214690 RZPD            57%
                              MPMGp1174 Paracentotus
                              lividus HGG
                              Paracentotus lividus
                              cDNA clone
                              MPMGp1174E1230Q 5',
                              mRNA sequence

               gi|91196436    AM205158 RZPD            54%
                              MPMGp1172 Paracentotus
                              lividus MBSB
                              Paracentotus lividus
                              cD.NA clone
                              MPMGp1172PI860Q 5',
                              mRNA sequence

               gi|89437392    AM188140 RZPD            91%
                              MPMGp1172 Paracentrous
                              lividus MBSB
                              Paracentrous lividus
                              cDNA clone
                              MPMGp1172D171Q 5',
                              mRNA sequence

               gi|109399528   133334291 Sea Urchin     93%
                              Lantern cDNA library
                              .Strongylocentrouce
                              purpuratus cDNA clone
                              LSP-2B1 5', mRNA
                              sequence

               gi|139265663   AM570670 RZPD            99%
                              MPMGp1173 Paracentrous
                              lividus GASPI.U
                              Paracentrous lividus
                              cDNA clone
                              MPMGp1173O02118Q 5',
                              mRNA sequence

               gi|139245731   AM557988 RZPD            97%
                              MPMGp1172 Paracentrous
                              lividus MBSB
                              Paracentrous lividus
                              cDNA clone
                              MPMGp1172M1164Q 5',
                              mRNA sequence

               gi|139268548   AM551774 RZPD           56s?
                              MPMGp1172 Paracentrous
                              lividus MBSB
                              Paracentrous lividus
                              cDNA clone
                              MPMGp1172PI7105Q 5',
                              mRNA sequence

               gi|139302769   AM539511 RZPD            96%
                              MPMGp1171 Paracentrous
                              lividus CLEB
                              Paracentrous lividus
                              cDNA clone
                              MPMGp1171E03207Q 5',
                              mRNA sequence

               gi|89446177    AM212199 RZPDMPMGpll74   99%
                              Paracentrous lividus
                              EGG Paracentrous
                              lividus cDNA clone
                              MPMGp1174G0224Q 5'
                              mRNA sequence

               gi|34746608    StrPu691.006926 Sea      97%
                              urchin larva cDNA
                              library MPMCp69l
                              Strongylocentrouce
                              purpuratus cDNA clone
                              MPMGp69101599; MPI
                              SURUDI_99015 5', mRNA
                              sequence

               gi|89440162    AM197614 RZPD            56%
                              MPMGp1172 Paracentrous
                              lividus MBSB
                              Paracentrous lividus
                              cDNA clone
                              MPMGp1172J1442Q 5'
                              mRNA sequence

               gi|139242372   AM545798 RZPD            83%
                              MPMGp1171 Paracentrous
                              lividus CLEB
                              Paracentrous lividus
                              cDNA clone
                              MPMGp1171F22200Q 5'
                              mRNA sequence

               gi|89438467    AM197503 RZPD            85%
                              MPMGp1172 Paracentrous
                              lividus MBSB
                              Paracentrous lividus
                              cDNA clone
                              MPMGp1172HI642Q 5',
                              mRNA sequence

               gi|l39268897   AM543994 RZPD            99%
                              MPMGp1171 Paracentrous
                              lividus CLEB
                              Paracentrous lividus
                              cDNA clone
                              MPMGpll71E13116Q 5',
                              mRNA sequence

               gi|91194567    AM194337 RZPD            99%
                              MPMGp1172 Paracentrous
                              lividus MBSB
                              Paracentrous lividus
                              cDNA clone
                              MPMGp1172L1134Q 5',
                              mRNA sequence

               gi|139255468   AM566312 RZPD            72%
                              MPMGp1173 Paracentrous
                              lividus GASPLU
                              Paracentrous lividus
                              cDNA clone
                              MPMGp1173C14108Q 5',
                              mRNA sequence

               gij 109401942  165424028 Sea Urchin     96%
                              Radial Nerve cDNA
                              Library
                              Strongylocentrouce
                              purpuratus cDNA clone
                              RNSP-9D11 5', mRNA
                              sequence

               gi|139313404   AM591951 RZPD            86%
                              MPMGpI173 Paracentrous
                              lividus GASPU.
                              Paracentrous lividus
                              cDNA clone
                              MPMGp1173E1357Q 5',
                              mRNA sequence

               gi|139357079   AM52S425 RZPD MPMGpl     73%
                              171 Paracentrous
                              lividus CLEB
                              Paracentrous lividus
                              cDNA clone
                              MPMGp1171.107203Q 5'
                              mRNA sequence

               gi|34745864    SirPuMI.006182 Sea       71%
                              urchin larva cDNA
                              library MPMGp691
                              Strongylocentrouce
                              purpuratus cDNA clone
                              MPMGp691L1867;
                              MPI_SURUDI_67L18 5'
                              mRNA sequence

               gi|34757996    StrPu621.003311 Sea      80%
                              urchin unfertilised
                              egg cDNA Library
                              MPMGpo21
                              Strongylocentrouce
                              purpuratus cDNA clone
                              MPMGp621K2014;
                              MPI_621_14K20 5'.
                              mRNA

               gi|139269763   AM527561 RZPD            58%
                              MPMGpll7l Paracentrous
                              lividus CLEB
                              Paracentrous lividus
                              cDNA clone
                              MPMGp117G05205Q 5'
                              mRNA sequence

               gi|57947733    ydf02cO3.yl Sea urchin   41%
                              EST Lib 1
                              Sironevlocentrotu.-.
                              purpura! us cDNA clone
                              ydf02c03 5'. mRNA
                              sequence

               gi|34748064    SlrPu691.008382 Sea      92%
                              urchin larva eDNA
                              library MPMGp69 1
                              Strongylocentrouce
                              purpuratus cDNA clone
                              MPMGp691K04142:
                              MPI_SURUDI_142K4 5',
                              mRNA sequence

               gi|139249974   AM560126 RZPD            90%
                              MPMGp1172
                              Paracentrotus lividus
                              MBSB Paracentrotus
                              lividus. cDNA clone
                              MPMGp1172U870Q 5'.
                              mRNA sequence

               gi|138478028   AM504745 RZPD            29%
                              MPMGp1171 Paracentrous
                              lividus CLEB
                              Paracentrous lividus
                              cDNA clone
                              MPMGp1171G242Q 5',
                              mRNA sequence

               gi|89442847    AM211931 RZPD            79%
                              MPMGp1174 Paracentrous
                              lividus KGG
                              Paracentrous lividus
                              cDNA clone
                              MPMGp1174J1323Q 5',
                              mRNA sequence

               gi|139279753   AM587839 RZPD            99%
                              MPMGp1173 Paracentrous
                              lividus GASPL.U
                              Paracentrous lividus
                              cDNA clone
                              MPMGp1173D1547Q 5',
                              mRNA sequence

               gi|139251073   AM568303 RZPD MPMGpl     80%
                              173 Paracentrous
                              lividus GASPLU
                              Paracentrous lividus
                              cDNA clone
                              MPMGp11731114112Q 5',
                              mRNA sequence

               gi|5713390     MPMGp621_035K08          85%
                              MPMGp621
                              Strongylocentrouce
                              purpuratus cDNA done
                              035KOK similar to
                              alpha-tubulin isotype
                              2. mRNA sequence

               gi|61136150    94026114 Sea Urchin      72%
                              primary mesenchyme
                              cell cDNA library
                              Strongylocentrouce
                              purpuratus cDNA clone
                              PMCSPR2-149L21 5',
                              mRNA sequence

               gi|l39254559   AM546I96 RZPD            61%
                              MPMGp1171 Paracentrous
                              lividus CLEB
                              Paracentrous lividus
                              cDNA done
                              MPMGp1171J06214Q 5',
                              mRNA sequence

               gi|139345636   AM542633 RZPD            59%
                              MPMGp1171 Paracentrous
                              lividus CLEB
                              Paracentrous lividus
                              cDNA clone
                              MPMGpl17IL17I09Q 5',
                              mRNA sequence

               gi|34751766    StrPu691.009QS4 Sea      98%
                              urchin larva cDNA
                              library MPMGp691
                              Strongylocentrouce
                              purpuratus cDNA clone
                              MPMGp691H4106:
                              MPI_SURUDI_106F14 5',
                              mRNA sequence

               gi|109396781   132763544 Sea Urchin     98%
                              Mesentary cDNA library
                              Strongylocentrouce
                              purpuratus cDNA clone
                              M.SP-1N8 3'. mRNA
                              sequence

               gi|139253277   AM56264I RZPD MPMGpl     59%
                              173 Paracentrous
                              lividus GASPLU
                              Paracentrous lividus
                              cDNA clone
                              MPMGp1173F112Q 5',.
                              mRNA sequence

               gi|l.39294700  AM579100 RZPD            70%
                              MPMGp1173 Paracentrous
                              lividus s GASPLU
                              Paracentrous lividus
                              cDNA clone
                              MPMGp1173.I2325Q 5',
                              mRNA sequence

               gi|61232318    92891311 Sea Urchin      43%
                              primary mesenchyme
                              cell cDNA librarv
                              Strongylocentrouce
                              purpuratus cDNA clone
                              PMCSPR2-73K21 5', mRNA
                              sequence

               gi|62377095    90959498 Sea Urchin      37%
                              primary mesenchyme
                              cell cDNA librarv
                              Strongylocentrouce
                              purpuratus cDNA clone
                              PMCSPR2-98N6 5', mRNA
                              sequence

               gi|62383655    92283899 Sea Urchin      89%
                              primary mesenchyme
                              cell cDNA librarv
                              Strongylocentrouce
                              purpuratus cDNA clone
                              PMCSPR2-164M9 5', mRNA
                              sequence

               gi|109399379   133201928 Sea Urchin     93%
                              Lantern cDNA library
                              Strongylocentrouce
                              purpuratus cDNA clone
                              LSP-1B23 5', mRNA
                              sequence

Uniprot_seaur  tr|Q6WQT5|     Toposome OS =
chin           Q6WQT5_PARU    Paracentrous lividus

               tr|Q3YL94|     Major yolk protein OS
               Q3YL94_STRNU   = Strongylocentrouce
                              purpuratus

               tr|Q2637S]     CyI actin OS =
               Q26378_TRIGR   Tripneustes gratilla

               tr|O18554|     Cytoplasmic actin CyII
               O18554_HELER   (Fragment) OS =
                              Heliocidaris
                              erythrogramma

               sp|P53474|     Actin.cytoskeletal 3A
               ACTE_STRPU     OS =
                              Strongylocentrouce
                              purpuratus

               tr|O18555|     Cytoplasmic actin CyII
               O18555_HELER   (Fragment) OS =
                              Heliocidaris
                              erythrogramma

               tr|Q8WQY0|     Toposome OS =
               Q8WQY0_TRIOR   Tripneustes gratilla

               tr|O18553|     Cytoplasmic actin CyII
               O18553_HFLTB   (Fragment) OS =
                              Heliocidaris
                              erythrogramma

               sp|Q07903|     Actin.cytoskeletal 2A
               ACTC_STRPU     OS =
                              Strongylocentrouce
                              purpuratus

               tr|Q70JA0|     Nectin OS =
               Q70JA0_PARLI   Paracentrous lividus

               tr|O44344|     Complemenl component
               O44344_STRFU   C3 OS =
                              Strongylocentrouce
                              purpuratus

               tr|Q86RA9|     Amassin OS =
               Q86RA9_STRPU   Strongylocentrouce
                              purpuratus

               tr|A9LYL6|     Putative uterine-ovary
               A9LYL6_HELTB   speeilie-44 protein OS
                              = Heliocidaris
                              tuberculata

               tr|Q6PTFl|     Fructose- bisphosphatc
               Q6PTF1_STRPU   aldolase (Fragment) OS
                              = Strongylocentrouce
                              purpuratus

               tr|D5H3J3|     Ubiquitin OS =
               D5H3J3_P5AMI   Psammechinus miliaris

               sp|P02290|     Histone H2B.1, sperm
               H2BS1_PARAN    OS = Parechinus
                              angulosus

               tr|Q6TS36|     Neural alfa2 tubulin
               Q6TS36_PARLI   OS = Paracentrous
                              lividus

               tr|O61230|     G-cadherin OS =
               O61230_LYTVA   Lytechinus variegatus

               tr|Q27753|     Histone 114 (Fragment)
               Q27753_PSAM1   OS = Psammechinus
                              miliaris

               tr|Q68BMl|     Outer arm dynein
               Q68BMLANTCR    binding protein OS =
                              Anthocidaris

               sp|Q05634|     Fascin OS =
               FASC_STRPU     Strongylocentrouce
                              purpuratus

               lr|Q8MPK6|     Beta- thymosin OS -
               QKMPK6 PARLI   Paracentrous lividus

               tr|Q963T2|     Early historic H3
               Q963T2_9ECHN   (Fragment) OS =
                              Tetrapygus mger

               Lr|B6DS92|     Fyn-related kinase OS
               B6DS92_STRPU   = Strongylocentrouce
                              purpuratus

               trlQSMXBO|     DNA- binding protein
               Q8MXB0_9HCHN   HOX10 (Fragment) OS =
                              Holopneustes
                              purpurescens

               trlQ25427|     USAI OS=Lytechinus
               Q25427_LYTVA   variegatus

               sp|Q27802|     Cytoplasmic dynein 2
               DYHC2_TRiGR    heavy chain 1 OS =
                              Tripneustes gratilla

               tr|Q6IUG6|     Axin OS - Lytechinus
               Q6IUG6_LYTVA   variegaius

Database       Accession      Protein name              E value
               number

EST_seaurchin  gi|50872995    Sp1170 Bacterially      5.0OE-17l
                              activated coelomocyle.
                              arrayed
                              Strorgvlocetrotus
                              purpuratus cDNA clone
                              Spl170 5' similar to
                              Actin, mKNA sequence

               gi|139309108   AM569285 RZPD           7.00E-158
                              MPMGp1173
                              Paracentrotus lividus
                              CASPLU Paracentrotus
                              lividus cDNA clone
                              MPMGp1173CI2I I5Q 5',
                              mRNA sequence

               gi|109400265   132678021 Sea Urchin    3.00E-179
                              Mesentary cDNA library
                              .Strongylocentrouce
                              purpuratus cDNA done
                              MSP-2L19 5', mRNA
                              sequence

               gi|24901528    RN.V/8T3 Sea urchin     5.00E-14l
                              radial nerve Lambda
                              Zap cDNA library
                              Heliocidaris
                              ervthrogramma cDNA 5',
                              similar to Cyl actin,
                              mRNA

               gi|5713384     MPMGp621_0341119         7.00E-73
                              MPMGp621
                              Strongylocentrouce
                              purpuratus to s cDNA
                              done 034H19 similar to
                              CyIIIa. mRNA sequence

               gi|89438622    AM2(MW54 RZPD            1.00E-44
                              MPMGp1172
                              Paracentrotus lividus
                              MBSB Paracentrotus
                              lividus cDNA done
                              MPMGp1172J2350Q 5',
                              mRNA

               gi|139330259   AM573430 RZPD            1.00E-45
                              MPMGp1173
                              Paracentrotus lividus
                              GASPLU Paracentrotus
                              lividus cDNA clone
                              MPMGp1173F08126Q 5',
                              mRNA

               gi|34756695    StrPu69 1.005010 Sea    3.00E-109
                              urchin larva cDNA
                              library MPMGp691
                              Strangylocentrotus
                              purpuratus cDNA clone
                              MPMGp69101838;
                              MPI_SURUDI 38018 5',
                              mRNA sequence

               gi|89445358    AM2IU987 RZPD            4.00K-56
                              MPMGp1172
                              Paracentrotus lividus
                              MBSB Paracenlrotus
                              liudus cD.NA Clone
                              MPMGp1172I0660Q 5',.
                              mRNA

               gi|89442983    AM2OH097 RZPD MPMGpl     4.00E-89
                              174 Paracentrotus
                              lividus EGG
                              Paracentotus lividus
                              cDNA clone
                              MPMGp1174D2214Q 5'.
                              mRNA

               gi|139328625   AM563391 RZPD           5.00E-123
                              MPMGp1173
                              Paracentrotus lividus
                              GASPLU Puracentratus
                              lividus cDNA clone
                              MPMGp1173E09100Q 5',
                              mRNA

               gi|109399080   133334354 Sea Urchin    3.00E-153
                              Lantern cDNA library
                              Strangylocentrotus
                              purpuratus cDNA clone
                              LSP-2F21 5', mRNA
                              sequence

               gi|62385757    92292867 Sea Urchin     3.00E-142
                              primary mesenchyme
                              cell cDNA J i bran
                              Strangylocentrotus
                              purpuralus cDNA clone
                              PMCSPR2-165P21 5',
                              mRNA

               gi|89457047    AM214690 RZPD            3.00E-33
                              MPMGp1174 Paracentotus
                              lividus HGG
                              Paracentotus lividus
                              cDNA clone
                              MPMGp1174E1230Q 5',
                              mRNA sequence

               gi|91196436    AM205158 RZPD            1.00E-45
                              MPMGp1172 Paracentotus
                              lividus MBSB
                              Paracentotus lividus
                              cD.NA clone
                              MPMGp1172PI860Q 5',
                              mRNA sequence

               gi|89437392    AM188140 RZPD            1.00E-70
                              MPMGp1172 Paracentrous
                              lividus MBSB
                              Paracentrous lividus
                              cDNA clone
                              MPMGp1172D171Q 5',
                              mRNA sequence

               gi|109399528   133334291 Sea Urchin    5.00E-122
                              Lantern cDNA library
                              .Strongylocentrouce
                              purpuratus cDNA clone
                              LSP-2B1 5', mRNA
                              sequence

               gi|139265663   AM570670 RZPD           8.00E-157
                              MPMGp1173 Paracentrous
                              lividus GASPI.U
                              Paracentrous lividus
                              cDNA clone
                              MPMGp1173O02118Q 5',
                              mRNA sequence

               gi|139245731   AM557988 RZPD           4.00E-108
                              MPMGp1172 Paracentrous
                              lividus MBSB
                              Paracentrous lividus
                              cDNA clone
                              MPMGp1172M1164Q 5',
                              mRNA sequence

               gi|139268548   AM551774 RZPD            6.00E-57
                              MPMGp1172 Paracentrous
                              lividus MBSB
                              Paracentrous lividus
                              cDNA clone
                              MPMGp1172PI7105Q 5',
                              mRNA sequence

               gi|139302769   AM539511 RZPD            3.00E-92
                              MPMGp1171 Paracentrous
                              lividus CLEB
                              Paracentrous lividus
                              cDNA clone
                              MPMGp1171E03207Q 5',
                              mRNA sequence

               gi|89446177    AM212199 RZPDMPMGpll74   1.00E-82
                              Paracentrous lividus
                              EGG Paracentrous
                              lividus cDNA clone
                              MPMGp1174G0224Q 5'
                              mRNA sequence

               gi|34746608    StrPu691.006926 Sea     6.00E-106
                              urchin larva cDNA
                              library MPMCp69l
                              Strongylocentrouce
                              purpuratus cDNA clone
                              MPMGp69101599; MPI
                              SURUDI_99015 5', mRNA
                              sequence

               gi|89440162    AM197614 RZPD            9.00E-54
                              MPMGp1172 Paracentrous
                              lividus MBSB
                              Paracentrous lividus
                              cDNA clone
                              MPMGp1172J1442Q 5'
                              mRNA sequence

               gi|139242372   AM545798 RZPD            2.00E-52
                              MPMGp1171 Paracentrous
                              lividus CLEB
                              Paracentrous lividus
                              cDNA clone
                              MPMGp1171F22200Q 5'
                              mRNA sequence

               gi|89438467    AM197503 RZPD            5.00E-95
                              MPMGp1172 Paracentrous
                              lividus MBSB
                              Paracentrous lividus
                              cDNA clone
                              MPMGp1172HI642Q 5',
                              mRNA sequence

               gi|l39268897   AM543994 RZPD           1.00E-155
                              MPMGp1171 Paracentrous
                              lividus CLEB
                              Paracentrous lividus
                              cDNA clone
                              MPMGpll71E13116Q 5',
                              mRNA sequence

               gi|91194567    AM194337 RZPD            3.00E-98
                              MPMGp1172 Paracentrous
                              lividus MBSB
                              Paracentrous lividus
                              cDNA clone
                              MPMGp1172L1134Q 5',
                              mRNA sequence

               gi|139255468   AM566312 RZPD            7.00E-41
                              MPMGp1173 Paracentrous
                              lividus GASPLU
                              Paracentrous lividus
                              cDNA clone
                              MPMGp1173C14108Q 5',
                              mRNA sequence

               gij 109401942  165424028 Sea Urchin    9.00E-163
                              Radial Nerve cDNA
                              Library
                              Strongylocentrouce
                              purpuratus cDNA clone
                              RNSP-9D11 5', mRNA
                              sequence

               gi|139313404   AM591951 RZPD            5.00E-83
                              MPMGpI173 Paracentrous
                              lividus GASPU.
                              Paracentrous lividus
                              cDNA clone
                              MPMGp1173E1357Q 5',
                              mRNA sequence

               gi|139357079   AM52S425 RZPD MPMGpl     2.00E-92
                              171 Paracentrous
                              lividus CLEB
                              Paracentrous lividus
                              cDNA clone
                              MPMGp1171.107203Q 5'
                              mRNA sequence

               gi|34745864    SirPuMI.006182 Sea       1.00E-94
                              urchin larva cDNA
                              library MPMGp691
                              Strongylocentrouce
                              purpuratus cDNA clone
                              MPMGp691L1867;
                              MPI_SURUDI_67L18 5'
                              mRNA sequence

               gi|34757996    StrPu621.003311 Sea      7.00E-70
                              urchin unfertilised
                              egg cDNA Library
                              MPMGpo21
                              Strongylocentrouce
                              purpuratus cDNA clone
                              MPMGp621K2014;
                              MPI_621_14K20 5'.
                              mRNA

               gi|139269763   AM527561 RZPD            1.00E-43
                              MPMGpll7l Paracentrous
                              lividus CLEB
                              Paracentrous lividus
                              cDNA clone
                              MPMGp117G05205Q 5'
                              mRNA sequence

               gi|57947733    ydf02cO3.yl Sea urchin   2.00E-16
                              EST Lib 1
                              Sironevlocentrotu.-.
                              purpuratus cDNA clone
                              ydf02c03 5'. mRNA
                              sequence

               gi|34748064    SlrPu691.008382 Sea     5.00E-104
                              urchin larva eDNA
                              library MPMGp69 1
                              Strongylocentrouce
                              purpuratus cDNA clone
                              MPMGp691K04142:
                              MPI_SURUDI_142K4 5',
                              mRNA sequence

               gi|139249974   AM560126 RZPD           2.00E-138
                              MPMGp1172
                              Paracentrotus lividus
                              MBSB Paracentrotus
                              lividus. cDNA clone
                              MPMGp1172U870Q 5'.
                              mRNA sequence

               gi|138478028   AM504745 RZPD            2.00E-38
                              MPMGp1171 Paracentrous
                              lividus CLEB
                              Paracentrous lividus
                              cDNA clone
                              MPMGp1171G242Q 5',
                              mRNA sequence

               gi|89442847    AM211931 RZPD            4.00E-51
                              MPMGp1174 Paracentrous
                              lividus KGG
                              Paracentrous lividus
                              cDNA clone
                              MPMGp1174J1323Q 5',
                              mRNA sequence

               gi|139279753   AM587839 RZPD           8.00E-121
                              MPMGp1173 Paracentrous
                              lividus GASPL.U
                              Paracentrous lividus
                              cDNA clone
                              MPMGp1173D1547Q 5',
                              mRNA sequence

               gi|139251073   AM568303 RZPD MPMGpl     1.00E-74
                              173 Paracentrous
                              lividus GASPLU
                              Paracentrous lividus
                              cDNA clone
                              MPMGp11731114112Q 5',
                              mRNA sequence

               gi|5713390     MPMGp621_035K08         5.00E-143
                              MPMGp621
                              Strongylocentrouce
                              purpuratus cDNA done
                              035KOK similar to
                              alpha-tubulin isotype
                              2. mRNA sequence

               gi|61136150    94026114 Sea Urchin     8.00E-100
                              primary mesenchyme
                              cell cDNA library
                              Strongylocentrouce
                              purpuratus cDNA clone
                              PMCSPR2-149L21 5',
                              mRNA sequence

               gi|l39254559   AM546I96 RZPD            1.00E-77
                              MPMGp1171 Paracentrous
                              lividus CLEB
                              Paracentrous lividus
                              cDNA done
                              MPMGp1171J06214Q 5',
                              mRNA sequence

               gi|139345636   AM542633 RZPD            8.00E-60
                              MPMGp1171 Paracentrous
                              lividus CLEB
                              Paracentrous lividus
                              cDNA clone
                              MPMGpl17IL17I09Q 5',
                              mRNA sequence

               gi|34751766    StrPu691.009QS4 Sea     2.00E-101
                              urchin larva cDNA
                              library MPMGp691
                              Strongylocentrouce
                              purpuratus cDNA clone
                              MPMGp691H4106:
                              MPI_SURUDI_106F14 5',
                              mRNA sequence

               gi|109396781   132763544 Sea Urchin    6.00E 158
                              Mesentary cDNA library
                              Strongylocentrouce
                              purpuratus cDNA clone
                              M.SP-1N8 3'. mRNA
                              sequence

               gi|139253277   AM56264I RZPD MPMGpl     2.00H-68
                              173 Paracentrous
                              lividus GASPLU
                              Paracentrous lividus
                              cDNA clone
                              MPMGp1173F112Q 5',.
                              mRNA sequence

               gi|l.39294700  AM579100 RZPD            3.00E-61
                              MPMGp1173 Paracentrous
                              lividus s GASPLU
                              Paracentrous lividus
                              cDNA clone
                              MPMGp1173.I2325Q 5',
                              mRNA sequence

               gi|61232318    92891311 Sea Urchin      6.00E-69
                              primary mesenchyme
                              cell cDNA librarv
                              Strongylocentrouce
                              purpuratus cDNA clone
                              PMCSPR2-73K21 5', mRNA
                              sequence

               gi|62377095    90959498 Sea Urchin     3.00E-143
                              primary mesenchyme
                              cell cDNA librarv
                              Strongylocentrouce
                              purpuratus cDNA clone
                              PMCSPR2-98N6 5', mRNA
                              sequence

               gi|62383655    92283899 Sea Urchin     4.00E-140
                              primary mesenchyme
                              cell cDNA librarv
                              Strongylocentrouce
                              purpuratus cDNA clone
                              PMCSPR2-164M9 5', mRNA
                              sequence

               gi|109399379   133201928 Sea Urchin            0
                              Lantern cDNA library
                              Strongylocentrouce
                              purpuratus cDNA clone
                              LSP-1B23 5', mRNA
                              sequence

Uniprot_seaur  tr|Q6WQT5|     Toposome OS =
chin           Q6WQT5_PARU    Paracentrous lividus

               tr|Q3YL94|     Major yolk protein OS
               Q3YL94_STRNU   = Strongylocentrouce
                              purpuratus

               tr|Q2637S]     CyI actin OS =
               Q26378_TRIGR   Tripneustes gratilla

               tr|O18554|     Cytoplasmic actin CyII
               O18554_HELER   (Fragment) OS =
                              Heliocidaris
                              erythrogramma

               sp|P53474|     Actin.cytoskeletal 3A
               ACTE_STRPU     OS =
                              Strongylocentrouce
                              purpuratus

               tr|O18555|     Cytoplasmic actin CyII
               O18555_HELER   (Fragment) OS =
                              Heliocidaris
                              erythrogramma

               tr|Q8WQY0|     Toposome OS =
               Q8WQY0_TRIOR   Tripneustes gratilla

               tr|O18553|     Cytoplasmic actin CyII
               O18553_HFLTB   (Fragment) OS =
                              Heliocidaris
                              erythrogramma

               sp|Q07903|     Actin.cytoskeletal 2A
               ACTC_STRPU     OS =
                              Strongylocentrouce
                              purpuratus

               tr|Q70JA0|     Nectin OS =
               Q70JA0_PARLI   Paracentrous lividus

               tr|O44344|     Complemenl component
               O44344_STRFU   C3 OS =
                              Strongylocentrouce
                              purpuratus

               tr|Q86RA9|     Amassin OS =
               Q86RA9_STRPU   Strongylocentrouce
                              purpuratus

               tr|A9LYL6|     Putative uterine-ovary
               A9LYL6_HELTB   speeilie-44 protein OS
                              = Heliocidaris
                              tuberculata

               tr|Q6PTFl|     Fructose- bisphosphatc
               Q6PTF1_STRPU   aldolase (Fragment) OS
                              = Strongylocentrouce
                              purpuratus

               tr|D5H3J3|     Ubiquitin OS =
               D5H3J3_P5AMI   Psammechinus miliaris

               sp|P02290|     Histone H2B.1, sperm
               H2BS1_PARAN    OS = Parechinus
                              angulosus

               tr|Q6TS36|     Neural alfa2 tubulin
               Q6TS36_PARLI   OS = Paracentrous
                              lividus

               tr|O61230|     G-cadherin OS =
               O61230_LYTVA   Lytechinus variegatus

               tr|Q27753|     Histone 114 (Fragment)
               Q27753_PSAM1   OS = Psammechinus
                              miliaris

               tr|Q68BMl|     Outer arm dynein
               Q68BMLANTCR    binding protein OS =
                              Anthocidaris

               sp|Q05634|     Fascin OS =
               FASC_STRPU     Strongylocentrouce
                              purpuratus

               lr|Q8MPK6|     Beta- thymosin OS -
               QKMPK6 PARLI   Paracentrous lividus

               tr|Q963T2|     Early historic H3
               Q963T2_9ECHN   (Fragment) OS =
                              Tetrapygus mger

               Lr|B6DS92|     Fyn-related kinase OS
               B6DS92_STRPU   = Strongylocentrouce
                              purpuratus

               trlQSMXBO|     DNA- binding protein
               Q8MXB0_9HCHN   HOX10 (Fragment) OS =
                              Holopneustes
                              purpurescens

               trlQ25427|     USAI OS=Lytechinus
               Q25427_LYTVA   variegatus

               sp|Q27802|     Cytoplasmic dynein 2
               DYHC2_TRiGR    heavy chain 1 OS =
                              Tripneustes gratilla

               tr|Q6IUG6|     Axin OS - Lytechinus
               Q6IUG6_LYTVA   variegaius

Database       Accession      Protein name             Max  pH7
               number

EST_seaurchin  gi|50872995    Sp1170 Bacterially       90%    x
                              activated coelomocyle.
                              arrayed
                              Strorgvlocetrotus
                              purpuratus cDNA clone
                              Spl170 5' similar to
                              Actin, mKNA sequence

               gi|139309108   AM569285 RZPD            99%    x
                              MPMGp1173
                              Paracentrotus lividus
                              CASPLU Paracentrotus
                              lividus cDNA clone
                              MPMGp1173CI2I I5Q 5',
                              mRNA sequence

               gi|109400265   132678021 Sea Urchin     97%    x
                              Mesentary cDNA library
                              .Strongylocentrouce
                              purpuratus cDNA done
                              MSP-2L19 5', mRNA
                              sequence

               gi|24901528    RN.V/8T3 Sea urchin      92%    x
                              radial nerve Lambda
                              Zap cDNA library
                              Heliocidaris
                              ervthrogramma cDNA 5',
                              similar to Cyl actin,
                              mRNA

               gi|5713384     MPMGp621_0341119         97%    x
                              MPMGp621
                              Strongylocentrouce
                              purpuratus to s cDNA
                              done 034H19 similar to
                              CyIIIa. mRNA sequence

               gi|89438622    AM2(MW54 RZPD            59%    x
                              MPMGp1172
                              Paracentrotus lividus
                              MBSB Paracentrotus
                              lividus cDNA done
                              MPMGp1172J2350Q 5',
                              mRNA

               gi|139330259   AM573430 RZPD            84%    x
                              MPMGp1173
                              Paracentrotus lividus
                              GASPLU Paracentrotus
                              lividus cDNA clone
                              MPMGp1173F08126Q 5',
                              mRNA

               gi|34756695    StrPu69 1.005010 Sea    100%
                              urchin larva cDNA
                              library MPMGp691
                              Strangylocentrotus
                              purpuratus cDNA clone
                              MPMGp69101838;
                              MPI_SURUDI 38018 5',
                              mRNA sequence

               gi|89445358    AM2IU987 RZPD            61%    x
                              MPMGp1172
                              Paracentrotus lividus
                              MBSB Paracenlrotus
                              liudus cD.NA Clone
                              MPMGp1172I0660Q 5',.
                              mRNA

               gi|89442983    AM2OH097 RZPD MPMGpl     82%    x
                              174 Paracentrotus
                              lividus EGG
                              Paracentotus lividus
                              cDNA clone
                              MPMGp1174D2214Q 5'.
                              mRNA

               gi|139328625   AM563391 RZPD            82%    x
                              MPMGp1173
                              Paracentrotus lividus
                              GASPLU Puracentratus
                              lividus cDNA clone
                              MPMGp1173E09100Q 5',
                              mRNA

               gi|109399080   133334354 Sea Urchin     92%
                              Lantern cDNA library
                              Strangylocentrotus
                              purpuratus cDNA clone
                              LSP-2F21 5', mRNA
                              sequence

               gi|62385757    92292867 Sea Urchin      99%
                              primary mesenchyme
                              cell cDNA J i bran
                              Strangylocentrotus
                              purpuralus cDNA clone
                              PMCSPR2-165P21 5',
                              mRNA

               gi|89457047    AM214690 RZPD            73%    x
                              MPMGp1174 Paracentotus
                              lividus HGG
                              Paracentotus lividus
                              cDNA clone
                              MPMGp1174E1230Q 5',
                              mRNA sequence

               gi|91196436    AM205158 RZPD            84%
                              MPMGp1172 Paracentotus
                              lividus MBSB
                              Paracentotus lividus
                              cD.NA clone
                              MPMGp1172PI860Q 5',
                              mRNA sequence

               gi|89437392    AM188140 RZPD            65%    x
                              MPMGp1172 Paracentrous
                              lividus MBSB
                              Paracentrous lividus
                              cDNA clone
                              MPMGp1172D171Q 5',
                              mRNA sequence

               gi|109399528   133334291 Sea Urchin    100%
                              Lantern cDNA library
                              .Strongylocentrouce
                              purpuratus cDNA clone
                              LSP-2B1 5', mRNA
                              sequence

               gi|139265663   AM570670 RZPD            94%    x
                              MPMGp1173 Paracentrous
                              lividus GASPI.U
                              Paracentrous lividus
                              cDNA clone
                              MPMGp1173O02118Q 5',
                              mRNA sequence

               gi|139245731   AM557988 RZPD            73%    x
                              MPMGp1172 Paracentrous
                              lividus MBSB
                              Paracentrous lividus
                              cDNA clone
                              MPMGp1172M1164Q 5',
                              mRNA sequence

               gi|139268548   AM551774 RZPD            69%    x
                              MPMGp1172 Paracentrous
                              lividus MBSB
                              Paracentrous lividus
                              cDNA clone
                              MPMGp1172PI7105Q 5',
                              mRNA sequence

               gi|139302769   AM539511 RZPD           74%.
                              MPMGp1171 Paracentrous
                              lividus CLEB
                              Paracentrous lividus
                              cDNA clone
                              MPMGp1171E03207Q 5',
                              mRNA sequence

               gi|89446177    AM212199 RZPDMPMGpll74   71%    x
                              Paracentrous lividus
                              EGG Paracentrous
                              lividus cDNA clone
                              MPMGp1174G0224Q 5'
                              mRNA sequence

               gi|34746608    StrPu691.006926 Sea     78'*    x
                              urchin larva cDNA
                              library MPMCp69l
                              Strongylocentrouce
                              purpuratus cDNA clone
                              MPMGp69101599; MPI
                              SURUDI_99015 5', mRNA
                              sequence

               gi|89440162    AM197614 RZPD            82%    x
                              MPMGp1172 Paracentrous
                              lividus MBSB
                              Paracentrous lividus
                              cDNA clone
                              MPMGp1172J1442Q 5'
                              mRNA sequence

               gi|139242372   AM545798 RZPD            51%    x
                              MPMGp1171 Paracentrous
                              lividus CLEB
                              Paracentrous lividus
                              cDNA clone
                              MPMGp1171F22200Q 5'
                              mRNA sequence

               gi|89438467    AM197503 RZPD            83%    x
                              MPMGp1172 Paracentrous
                              lividus MBSB
                              Paracentrous lividus
                              cDNA clone
                              MPMGp1172HI642Q 5',
                              mRNA sequence

               gi|l39268897   AM543994 RZPD            88%    x
                              MPMGp1171 Paracentrous
                              lividus CLEB
                              Paracentrous lividus
                              cDNA clone
                              MPMGpll71E13116Q 5',
                              mRNA sequence

               gi|91194567    AM194337 RZPD            75%    x
                              MPMGp1172 Paracentrous
                              lividus MBSB
                              Paracentrous lividus
                              cDNA clone
                              MPMGp1172L1134Q 5',
                              mRNA sequence

               gi|139255468   AM566312 RZPD            46%    x
                              MPMGp1173 Paracentrous
                              lividus GASPLU
                              Paracentrous lividus
                              cDNA clone
                              MPMGp1173C14108Q 5',
                              mRNA sequence

               gij 109401942  165424028 Sea Urchin    100%    x
                              Radial Nerve cDNA
                              Library
                              Strongylocentrouce
                              purpuratus cDNA clone
                              RNSP-9D11 5', mRNA
                              sequence

               gi|139313404   AM591951 RZPD            74%    x
                              MPMGpI173 Paracentrous
                              lividus GASPU.
                              Paracentrous lividus
                              cDNA clone
                              MPMGp1173E1357Q 5',
                              mRNA sequence

               gi|139357079   AM52S425 RZPD MPMGpl     83%
                              171 Paracentrous
                              lividus CLEB
                              Paracentrous lividus
                              cDNA clone
                              MPMGp1171.107203Q 5'
                              mRNA sequence

               gi|34745864    SirPuMI.006182 Sea       99%    x
                              urchin larva cDNA
                              library MPMGp691
                              Strongylocentrouce
                              purpuratus cDNA clone
                              MPMGp691L1867;
                              MPI_SURUDI_67L18 5'
                              mRNA sequence

               gi|34757996    StrPu621.003311 Sea      94%
                              urchin unfertilised
                              egg cDNA Library
                              MPMGpo21
                              Strongylocentrouce
                              purpuratus cDNA clone
                              MPMGp621K2014;
                              MPI_621_14K20 5'.
                              mRNA

               gi|139269763   AM527561 RZPD            51%    x
                              MPMGpll7l Paracentrous
                              lividus CLEB
                              Paracentrous lividus
                              cDNA clone
                              MPMGp117G05205Q 5'
                              mRNA sequence

               gi|57947733    ydf02cO3.yl Sea urchin   75%    x
                              EST Lib 1
                              Sironevlocentrotu.-.
                              purpura! us cDNA clone
                              ydf02c03 5'. mRNA
                              sequence

               gi|34748064    SlrPu691.008382 Sea      98%
                              urchin larva eDNA
                              library MPMGp69 1
                              Strongylocentrouce
                              purpuratus cDNA clone
                              MPMGp691K04142:
                              MPI_SURUDI_142K4 5',
                              mRNA sequence

               gi|139249974   AM560126 RZPD            80%    x
                              MPMGp1172
                              Paracentrotus lividus
                              MBSB Paracentrotus
                              lividus. cDNA clone
                              MPMGp1172U870Q 5'.
                              mRNA sequence

               gi|138478028   AM504745 RZPD           100%    x
                              MPMGp1171 Paracentrous
                              lividus CLEB
                              Paracentrous lividus
                              cDNA clone
                              MPMGp1171G242Q 5',
                              mRNA sequence

               gi|89442847    AM211931 RZPD            51%
                              MPMGp1174 Paracentrous
                              lividus KGG
                              Paracentrous lividus
                              cDNA clone
                              MPMGp1174J1323Q 5',
                              mRNA sequence

               gi|139279753   AM587839 RZPD            82%    x
                              MPMGp1173 Paracentrous
                              lividus GASPL.U
                              Paracentrous lividus
                              cDNA clone
                              MPMGp1173D1547Q 5',
                              mRNA sequence

               gi|139251073   AM568303 RZPD MPMGpl     80%    x
                              173 Paracentrous
                              lividus GASPLU
                              Paracentrous lividus
                              cDNA clone
                              MPMGp11731114112Q 5',
                              mRNA sequence

               gi|5713390     MPMGp621_035K08          96%    x
                              MPMGp621
                              Strongylocentrouce
                              purpuratus cDNA done
                              035KOK similar to
                              alpha-tubulin isotype
                              2. mRNA sequence

               gi|61136150    94026114 Sea Urchin      97%    x
                              primary mesenchyme
                              cell cDNA library
                              Strongylocentrouce
                              purpuratus cDNA clone
                              PMCSPR2-149L21 5',
                              mRNA sequence

               gi|l39254559   AM546I96 RZPD            99%
                              MPMGp1171 Paracentrous
                              lividus CLEB
                              Paracentrous lividus
                              cDNA done
                              MPMGp1171J06214Q 5',
                              mRNA sequence

               gi|139345636   AM542633 RZPD            77%
                              MPMGp1171 Paracentrous
                              lividus CLEB
                              Paracentrous lividus
                              cDNA clone
                              MPMGpl17IL17I09Q 5',
                              mRNA sequence

               gi|34751766    StrPu691.009QS4 Sea      83%
                              urchin larva cDNA
                              library MPMGp691
                              Strongylocentrouce
                              purpuratus cDNA clone
                              MPMGp691H4106:
                              MPI_SURUDI_106F14 5',
                              mRNA sequence

               gi|109396781   132763544 Sea Urchin     92%
                              Mesentary cDNA library
                              Strongylocentrouce
                              purpuratus cDNA clone
                              M.SP-1N8 3'. mRNA
                              sequence

               gi|139253277   AM56264I RZPD MPMGpl     80%
                              173 Paracentrous
                              lividus GASPLU
                              Paracentrous lividus
                              cDNA clone
                              MPMGp1173F112Q 5',.
                              mRNA sequence

               gi|l.39294700  AM579100 RZPD            89%
                              MPMGp1173 Paracentrous
                              lividus s GASPLU
                              Paracentrous lividus
                              cDNA clone
                              MPMGp1173.I2325Q 5',
                              mRNA sequence

               gi|61232318    92891311 Sea Urchin      95%
                              primary mesenchyme
                              cell cDNA librarv
                              Strongylocentrouce
                              purpuratus cDNA clone
                              PMCSPR2-73K21 5', mRNA
                              sequence

               gi|62377095    90959498 Sea Urchin      96%
                              primary mesenchyme
                              cell cDNA librarv
                              Strongylocentrouce
                              purpuratus cDNA clone
                              PMCSPR2-98N6 5', mRNA
                              sequence

               gi|62383655    92283899 Sea Urchin      96%
                              primary mesenchyme
                              cell cDNA librarv
                              Strongylocentrouce
                              purpuratus cDNA clone
                              PMCSPR2-164M9 5', mRNA
                              sequence

               gi|109399379   133201928 Sea Urchin     96%
                              Lantern cDNA library
                              Strongylocentrouce
                              purpuratus cDNA clone
                              LSP-1B23 5', mRNA
                              sequence

Uniprot_seaur  tr|Q6WQT5|     Toposome OS =                   x
chin           Q6WQT5_PARU    Paracentrous lividus

               tr|Q3YL94|     Major yolk protein OS           x
               Q3YL94_STRNU   = Strongylocentrouce
                              purpuratus

               tr|Q2637S]     CyI actin OS =
               Q26378_TRIGR   Tripneustes gratilla

               tr|O18554|     Cytoplasmic actin CyII
               O18554_HELER   (Fragment) OS =
                              Heliocidaris
                              erythrogramma

               sp|P53474|     Actin.cytoskeletal 3A
               ACTE_STRPU     OS =
                              Strongylocentrouce
                              purpuratus

               tr|O18555|     Cytoplasmic actin CyII
               O18555_HELER   (Fragment) OS =
                              Heliocidaris
                              erythrogramma

               tr|Q8WQY0|     Toposome OS =                   x
               Q8WQY0_TRIOR   Tripneustes gratilla

               tr|O18553|     Cytoplasmic actin CyII          x
               O18553_HFLTB   (Fragment) OS =
                              Heliocidaris
                              erythrogramma

               sp|Q07903|     Actin.cytoskeletal 2A           x
               ACTC_STRPU     OS =
                              Strongylocentrouce
                              purpuratus

               tr|Q70JA0|     Nectin OS =                     x
               Q70JA0_PARLI   Paracentrous lividus

               tr|O44344|     Complemenl component            x
               O44344_STRFU   C3 OS =
                              Strongylocentrouce
                              purpuratus

               tr|Q86RA9|     Amassin OS =                    x
               Q86RA9_STRPU   Strongylocentrouce
                              purpuratus

               tr|A9LYL6|     Putative uterine-ovary          x
               A9LYL6_HELTB   speeilie-44 protein OS
                              = Heliocidaris
                              tuberculata

               tr|Q6PTFl|     Fructose- bisphosphatc          x
               Q6PTF1_STRPU   aldolase (Fragment) OS
                              = Strongylocentrouce
                              purpuratus

               tr|D5H3J3|     Ubiquitin OS =
               D5H3J3_P5AMI   Psammechinus miliaris

               sp|P02290|     Histone H2B.1, sperm            x
               H2BS1_PARAN    OS = Parechinus
                              angulosus

               tr|Q6TS36|     Neural alfa2 tubulin            x
               Q6TS36_PARLI   OS = Paracentrous
                              lividus

               tr|O61230|     G-cadherin OS =                 x
               O61230_LYTVA   Lytechinus variegatus

               tr|Q27753|     Histone 114 (Fragment)          x
               Q27753_PSAM1   OS = Psammechinus
                              miliaris

               tr|Q68BMl|     Outer arm dynein
               Q68BMLANTCR    binding protein OS =
                              Anthocidaris

               sp|Q05634|     Fascin OS =                     x
               FASC_STRPU     Strongylocentrouce
                              purpuratus

               lr|Q8MPK6|     Beta- thymosin OS -             x
               QKMPK6 PARLI   Paracentrous lividus

               tr|Q963T2|     Early historic H3               x
               Q963T2_9ECHN   (Fragment) OS =
                              Tetrapygus mger

               Lr|B6DS92|     Fyn-related kinase OS           x
               B6DS92_STRPU   = Strongylocentrouce
                              purpuratus

               trlQSMXBO|     DNA- binding protein            x
               Q8MXB0_9HCHN   HOX10 (Fragment) OS =
                              Holopneustes
                              purpurescens

               trlQ25427|     USAI OS=Lytechinus
               Q25427_LYTVA   variegatus

               sp|Q27802|     Cytoplasmic dynein 2            x
               DYHC2_TRiGR    heavy chain 1 OS =
                              Tripneustes gratilla

               tr|Q6IUG6|     Axin OS - Lytechinus            x
               Q6IUG6_LYTVA   variegaius

Database       Accession      Protein name            ctrl  pH2.2
               number

EST_seaurchin  gi|50872995    Sp1170 Bacterially         x
                              activated coelomocyle.
                              arrayed
                              Strorgvlocetrotus
                              purpuratus cDNA clone
                              Spl170 5' similar to
                              Actin, mKNA sequence

               gi|139309108   AM569285 RZPD              x
                              MPMGp1173
                              Paracentrotus lividus
                              CASPLU Paracentrotus
                              lividus cDNA clone
                              MPMGp1173CI2I I5Q 5',
                              mRNA sequence

               gi|109400265   132678021 Sea Urchin
                              Mesentary cDNA library
                              .Strongylocentrouce
                              purpuratus cDNA done
                              MSP-2L19 5', mRNA
                              sequence

               gi|24901528    RN.V/8T3 Sea urchin
                              radial nerve Lambda
                              Zap cDNA library
                              Heliocidaris
                              ervthrogramma cDNA 5',
                              similar to Cyl actin,
                              mRNA

               gi|5713384     MPMGp621_0341119
                              MPMGp621
                              Strongylocentrouce
                              purpuratus to s cDNA
                              done 034H19 similar to
                              CyIIIa. mRNA sequence

               gi|89438622    AM2(MW54 RZPD              x
                              MPMGp1172
                              Paracentrotus lividus
                              MBSB Paracentrotus
                              lividus cDNA done
                              MPMGp1172J2350Q 5',
                              mRNA

               gi|139330259   AM573430 RZPD
                              MPMGp1173
                              Paracentrotus lividus
                              GASPLU Paracentrotus
                              lividus cDNA clone
                              MPMGp1173F08126Q 5',
                              mRNA

               gi|34756695    StrPu69 1.005010 Sea              x
                              urchin larva cDNA
                              library MPMGp691
                              Strangylocentrotus
                              purpuratus cDNA clone
                              MPMGp69101838;
                              MPI_SURUDI 38018 5',
                              mRNA sequence

               gi|89445358    AM2IU987 RZPD                     x
                              MPMGp1172
                              Paracentrotus lividus
                              MBSB Paracenlrotus
                              liudus cD.NA Clone
                              MPMGp1172I0660Q 5',.
                              mRNA

               gi|89442983    AM2OH097 RZPD MPMGpl
                              174 Paracentrotus
                              lividus EGG
                              Paracentotus lividus
                              cDNA clone
                              MPMGp1174D2214Q 5'.
                              mRNA

               gi|139328625   AM563391 RZPD                     x
                              MPMGp1173
                              Paracentrotus lividus
                              GASPLU Puracentratus
                              lividus cDNA clone
                              MPMGp1173E09100Q 5',
                              mRNA

               gi|109399080   133334354 Sea Urchin       x
                              Lantern cDNA library
                              Strangylocentrotus
                              purpuratus cDNA clone
                              LSP-2F21 5', mRNA
                              sequence

               gi|62385757    92292867 Sea Urchin        x
                              primary mesenchyme
                              cell cDNA J i bran
                              Strangylocentrotus
                              purpuralus cDNA clone
                              PMCSPR2-165P21 5',
                              mRNA

               gi|89457047    AM214690 RZPD              x
                              MPMGp1174 Paracentotus
                              lividus HGG
                              Paracentotus lividus
                              cDNA clone
                              MPMGp1174E1230Q 5',
                              mRNA sequence

               gi|91196436    AM205158 RZPD                     x
                              MPMGp1172 Paracentotus
                              lividus MBSB
                              Paracentotus lividus
                              cD.NA clone
                              MPMGp1172PI860Q 5',
                              mRNA sequence

               gi|89437392    AM188140 RZPD
                              MPMGp1172 Paracentrous
                              lividus MBSB
                              Paracentrous lividus
                              cDNA clone
                              MPMGp1172D171Q 5',
                              mRNA sequence

               gi|109399528   133334291 Sea Urchin       x
                              Lantern cDNA library
                              .Strongylocentrouce
                              purpuratus cDNA clone
                              LSP-2B1 5', mRNA
                              sequence

               gi|139265663   AM570670 RZPD
                              MPMGp1173 Paracentrous
                              lividus GASPI.U
                              Paracentrous lividus
                              cDNA clone
                              MPMGp1173O02118Q 5',
                              mRNA sequence

               gi|139245731   AM557988 RZPD              x      x
                              MPMGp1172 Paracentrous
                              lividus MBSB
                              Paracentrous lividus
                              cDNA clone
                              MPMGp1172M1164Q 5',
                              mRNA sequence

               gi|139268548   AM551774 RZPD
                              MPMGp1172 Paracentrous
                              lividus MBSB
                              Paracentrous lividus
                              cDNA clone
                              MPMGp1172PI7105Q 5',
                              mRNA sequence

               gi|139302769   AM539511 RZPD              x
                              MPMGp1171 Paracentrous
                              lividus CLEB
                              Paracentrous lividus
                              cDNA clone
                              MPMGp1171E03207Q 5',
                              mRNA sequence

               gi|89446177    AM212199 RZPDMPMGpll74     x
                              Paracentrous lividus
                              EGG Paracentrous
                              lividus cDNA clone
                              MPMGp1174G0224Q 5'
                              mRNA sequence

               gi|34746608    StrPu691.006926 Sea
                              urchin larva cDNA
                              library MPMCp69l
                              Strongylocentrouce
                              purpuratus cDNA clone
                              MPMGp69101599; MPI
                              SURUDI_99015 5', mRNA
                              sequence

               gi|89440162    AM197614 RZPD
                              MPMGp1172 Paracentrous
                              lividus MBSB
                              Paracentrous lividus
                              cDNA clone
                              MPMGp1172J1442Q 5'
                              mRNA sequence

               gi|139242372   AM545798 RZPD
                              MPMGp1171 Paracentrous
                              lividus CLEB
                              Paracentrous lividus
                              cDNA clone
                              MPMGp1171F22200Q 5'
                              mRNA sequence

               gi|89438467    AM197503 RZPD                     x
                              MPMGp1172 Paracentrous
                              lividus MBSB
                              Paracentrous lividus
                              cDNA clone
                              MPMGp1172HI642Q 5',
                              mRNA sequence

               gi|l39268897   AM543994 RZPD
                              MPMGp1171 Paracentrous
                              lividus CLEB
                              Paracentrous lividus
                              cDNA clone
                              MPMGpll71E13116Q 5',
                              mRNA sequence

               gi|91194567    AM194337 RZPD
                              MPMGp1172 Paracentrous
                              lividus MBSB
                              Paracentrous lividus
                              cDNA clone
                              MPMGp1172L1134Q 5',
                              mRNA sequence

               gi|139255468   AM566312 RZPD
                              MPMGp1173 Paracentrous
                              lividus GASPLU
                              Paracentrous lividus
                              cDNA clone
                              MPMGp1173C14108Q 5',
                              mRNA sequence

               gij 109401942  165424028 Sea Urchin
                              Radial Nerve cDNA
                              Library
                              Strongylocentrouce
                              purpuratus cDNA clone
                              RNSP-9D11 5', mRNA
                              sequence

               gi|139313404   AM591951 RZPD
                              MPMGpI173 Paracentrous
                              lividus GASPU.
                              Paracentrous lividus
                              cDNA clone
                              MPMGp1173E1357Q 5',
                              mRNA sequence

               gi|139357079   AM52S425 RZPD MPMGpl       x
                              171 Paracentrous
                              lividus CLEB
                              Paracentrous lividus
                              cDNA clone
                              MPMGp1171.107203Q 5'
                              mRNA sequence

               gi|34745864    SirPuMI.006182 Sea
                              urchin larva cDNA
                              library MPMGp691
                              Strongylocentrouce
                              purpuratus cDNA clone
                              MPMGp691L1867;
                              MPI_SURUDI_67L18 5'
                              mRNA sequence

               gi|34757996    StrPu621.003311 Sea               x
                              urchin unfertilised
                              egg cDNA Library
                              MPMGpo21
                              Strongylocentrouce
                              purpuratus cDNA clone
                              MPMGp621K2014;
                              MPI_621_14K20 5'.
                              mRNA

               gi|139269763   AM527561 RZPD
                              MPMGpll7l Paracentrous
                              lividus CLEB
                              Paracentrous lividus
                              cDNA clone
                              MPMGp117G05205Q 5'
                              mRNA sequence

               gi|57947733    ydf02cO3.yl Sea urchin     x
                              EST Lib 1
                              Sironevlocentrotu.-.
                              purpura! us cDNA clone
                              ydf02c03 5'. mRNA
                              sequence

               gi|34748064    SlrPu691.008382 Sea
                              urchin larva eDNA
                              library MPMGp69 1
                              Strongylocentrouce
                              purpuratus cDNA clone
                              MPMGp691K04142:
                              MPI_SURUDI_142K4 5',
                              mRNA sequence

               gi|139249974   AM560126 RZPD
                              MPMGp1172
                              Paracentrotus lividus
                              MBSB Paracentrotus
                              lividus. cDNA clone
                              MPMGp1172U870Q 5'.
                              mRNA sequence

               gi|138478028   AM504745 RZPD              x
                              MPMGp1171 Paracentrous
                              lividus CLEB
                              Paracentrous lividus
                              cDNA clone
                              MPMGp1171G242Q 5',
                              mRNA sequence

               gi|89442847    AM211931 RZPD              x
                              MPMGp1174 Paracentrous
                              lividus KGG
                              Paracentrous lividus
                              cDNA clone
                              MPMGp1174J1323Q 5',
                              mRNA sequence

               gi|139279753   AM587839 RZPD
                              MPMGp1173 Paracentrous
                              lividus GASPL.U
                              Paracentrous lividus
                              cDNA clone
                              MPMGp1173D1547Q 5',
                              mRNA sequence

               gi|139251073   AM568303 RZPD MPMGpl
                              173 Paracentrous
                              lividus GASPLU
                              Paracentrous lividus
                              cDNA clone
                              MPMGp11731114112Q 5',
                              mRNA sequence

               gi|5713390     MPMGp621_035K08
                              MPMGp621
                              Strongylocentrouce
                              purpuratus cDNA done
                              035KOK similar to
                              alpha-tubulin isotype
                              2. mRNA sequence

               gi|61136150    94026114 Sea Urchin        x
                              primary mesenchyme
                              cell cDNA library
                              Strongylocentrouce
                              purpuratus cDNA clone
                              PMCSPR2-149L21 5',
                              mRNA sequence

               gi|l39254559   AM546I96 RZPD              x
                              MPMGp1171 Paracentrous
                              lividus CLEB
                              Paracentrous lividus
                              cDNA done
                              MPMGp1171J06214Q 5',
                              mRNA sequence

               gi|139345636   AM542633 RZPD                     x
                              MPMGp1171 Paracentrous
                              lividus CLEB
                              Paracentrous lividus
                              cDNA clone
                              MPMGpl17IL17I09Q 5',
                              mRNA sequence

               gi|34751766    StrPu691.009QS4 Sea               x
                              urchin larva cDNA
                              library MPMGp691
                              Strongylocentrouce
                              purpuratus cDNA clone
                              MPMGp691H4106:
                              MPI_SURUDI_106F14 5',
                              mRNA sequence

               gi|109396781   132763544 Sea Urchin       x
                              Mesentary cDNA library
                              Strongylocentrouce
                              purpuratus cDNA clone
                              M.SP-1N8 3'. mRNA
                              sequence

               gi|139253277   AM56264I RZPD MPMGpl       x
                              173 Paracentrous
                              lividus GASPLU
                              Paracentrous lividus
                              cDNA clone
                              MPMGp1173F112Q 5',.
                              mRNA sequence

               gi|l.39294700  AM579100 RZPD                     x
                              MPMGp1173 Paracentrous
                              lividus s GASPLU
                              Paracentrous lividus
                              cDNA clone
                              MPMGp1173.I2325Q 5',
                              mRNA sequence

               gi|61232318    92891311 Sea Urchin               x
                              primary mesenchyme
                              cell cDNA librarv
                              Strongylocentrouce
                              purpuratus cDNA clone
                              PMCSPR2-73K21 5', mRNA
                              sequence

               gi|62377095    90959498 Sea Urchin               x
                              primary mesenchyme
                              cell cDNA librarv
                              Strongylocentrouce
                              purpuratus cDNA clone
                              PMCSPR2-98N6 5', mRNA
                              sequence

               gi|62383655    92283899 Sea Urchin        x
                              primary mesenchyme
                              cell cDNA librarv
                              Strongylocentrouce
                              purpuratus cDNA clone
                              PMCSPR2-164M9 5', mRNA
                              sequence

               gi|109399379   133201928 Sea Urchin       x
                              Lantern cDNA library
                              Strongylocentrouce
                              purpuratus cDNA clone
                              LSP-1B23 5', mRNA
                              sequence

Uniprot_seaur  tr|Q6WQT5|     Toposome OS =
chin           Q6WQT5_PARU    Paracentrous lividus

               tr|Q3YL94|     Major yolk protein OS      x
               Q3YL94_STRNU   = Strongylocentrouce
                              purpuratus

               tr|Q2637S]     CyI actin OS =             x
               Q26378_TRIGR   Tripneustes gratilla

               tr|O18554|     Cytoplasmic actin CyII     x
               O18554_HELER   (Fragment) OS =
                              Heliocidaris
                              erythrogramma

               sp|P53474|     Actin.cytoskeletal 3A             x
               ACTE_STRPU     OS =
                              Strongylocentrouce
                              purpuratus

               tr|O18555|     Cytoplasmic actin CyII            x
               O18555_HELER   (Fragment) OS =
                              Heliocidaris
                              erythrogramma

               tr|Q8WQY0|     Toposome OS =
               Q8WQY0_TRIOR   Tripneustes gratilla

               tr|O18553|     Cytoplasmic actin CyII
               O18553_HFLTB   (Fragment) OS =
                              Heliocidaris
                              erythrogramma

               sp|Q07903|     Actin.cytoskeletal 2A             x
               ACTC_STRPU     OS =
                              Strongylocentrouce
                              purpuratus

               tr|Q70JA0|     Nectin OS =                x      x
               Q70JA0_PARLI   Paracentrous lividus

               tr|O44344|     Complemenl component       x      x
               O44344_STRFU   C3 OS =
                              Strongylocentrouce
                              purpuratus

               tr|Q86RA9|     Amassin OS =               x
               Q86RA9_STRPU   Strongylocentrouce
                              purpuratus

               tr|A9LYL6|     Putative uterine-ovary
               A9LYL6_HELTB   speeilie-44 protein OS
                              = Heliocidaris
                              tuberculata

               tr|Q6PTFl|     Fructose- bisphosphatc
               Q6PTF1_STRPU   aldolase (Fragment) OS
                              = Strongylocentrouce
                              purpuratus

               tr|D5H3J3|     Ubiquitin OS =                    x
               D5H3J3_P5AMI   Psammechinus miliaris

               sp|P02290|     Histone H2B.1, sperm
               H2BS1_PARAN    OS = Parechinus
                              angulosus

               tr|Q6TS36|     Neural alfa2 tubulin       x
               Q6TS36_PARLI   OS = Paracentrous
                              lividus

               tr|O61230|     G-cadherin OS =            x      x
               O61230_LYTVA   Lytechinus variegatus

               tr|Q27753|     Histone 114 (Fragment)
               Q27753_PSAM1   OS = Psammechinus
                              miliaris

               tr|Q68BMl|     Outer arm dynein                  x
               Q68BMLANTCR    binding protein OS =
                              Anthocidaris

               sp|Q05634|     Fascin OS =
               FASC_STRPU     Strongylocentrouce
                              purpuratus

               lr|Q8MPK6|     Beta- thymosin OS -        x      x
               QKMPK6 PARLI   Paracentrous lividus

               tr|Q963T2|     Early historic H3
               Q963T2_9ECHN   (Fragment) OS =
                              Tetrapygus mger

               Lr|B6DS92|     Fyn-related kinase OS             x
               B6DS92_STRPU   = Strongylocentrouce
                              purpuratus

               trlQSMXBO|     DNA- binding protein
               Q8MXB0_9HCHN   HOX10 (Fragment) OS =
                              Holopneustes
                              purpurescens

               trlQ25427|     USAI OS=Lytechinus                x
               Q25427_LYTVA   variegatus

               sp|Q27802|     Cytoplasmic dynein 2       x      x
               DYHC2_TRiGR    heavy chain 1 OS =
                              Tripneustes gratilla

               tr|Q6IUG6|     Axin OS - Lytechinus
               Q6IUG6_LYTVA   variegaius
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Article Details
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Author:Fasoli, Elisa; D'Amato, Alfonsina; Righetti, Pier Giorgio; Barbieri, Rainer; Bellavial, Daniele
Publication:The Biological Bulletin
Article Type:Report
Geographic Code:4EUIT
Date:Apr 1, 2012
Words:14916
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