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Evaluation of the effect of smoking on complete blood counts serum C-reactive protein and magnesium levels in healthy adult male smokers.

Byline: Fatehuddin Khand Shumaila Shabbir Shaikh Muhammad Atif Ata and Saeed Sattar Shaikh

Abstract

Objective: To examine the effect of smoking on complete blood count serum C-reactive protein and magnesium levels in male smokers.

Methods: The prospective case-control study was conducted in two villages of Matiari district in rural Sindh Pakistan from July to December 2013 and comprised healthy adult male smokers and an equal number of matching non-smokers as controls. The complete blood count serum C-reactive protein and magnesium levels in all the subjects were measured to assess the effect of smoking on these parameters.

Results: The two groups had 48 subjects each with an overall age range of 20-40 years. The results of complete blood count were comparable except for lymphocyte which was significantly higher (pless than 0.001) and neutrophil which was lower (pless than 0.001) in smokers than in the non-smokers. Serum C-reactive protein concentrations among the cases (14.620.16mg/L) compared to the controls (4.810.38mg/L) were significantly higher (pless than 0.001). However reverse was true for serum magnesium levels which were significantly higher (pless than 0.001) in the controls (2.520.18mg/L) as against the cases (1.090.38mg/dl).Serum C-reactive protein-to-magnesium ratio was significantly higher (pless than 0.001) in smokers than in the non-smokers.

Conclusion: Lymphocyte count was higher while neutrophil count was lower in smokers. Smoking also caused significant increase in serum C-reactive protein concentration concomitant to decrease in magnesium concentration in the smokers.

Keywords: Smoking Complete blood counts C-reactive protein Magnesium. (JPMA 65: 59; 2015)

Introduction

Cigarette smoking is a major risk factor involved in the pathogenesis of several diseases with an inflammatory component such as cardiovascular disease (CVD) and chronic obstructive pulmonary disease (COPD).12

Smoking triggers inflammation which is manifested by raised plasma levels of inflammatory markers like C- reactive protein (CRP) and white blood cell ( WBC) counts.34 CRP synthesis increases dramatically in the liver in response to cytokines released by adipocytes5 and macrophages.6 In a recent study it has been observed that adult males who were current smokers had elevated levels of serum CRP compared to non- smokers.7 It has also been noted that people with high basal levels of CRP are at increased risk of developing obesity diabetes hypertension and CVD.89 Healthy smokers compared to non-smokers have been found to have more insulin resistance (a characteristic of metabolic syndrome that is caused by lower cellular magnesium) increased glucose levels much raised insulin levels almost twice more very low-density lipoprotein (VLDL) cholesterol and 30 percent lower high-density lipoprotein(HDL) cholesterol.

Although these variables are not direct measures of magnesium status in smokers but they clearly point out that smoking lowers cellular magnesium levels.10 There seems to be a link between hypomagnesaemia and elevated plasma levels of CRP in cellular processes that affect vascular endothelial function11 in people with obesity12 and in people with blood pressure (BP) alterations.13

The current study was planned to find out the effect of smoking on complete blood count (CBC) and serum levels of CRP and magnesium in healthy adult male smokers. To our knowledge no study has concurrently examined the effect of smoking on CBC and blood levels of magnesium and CRP in healthy adult male smokers residing in rural areas.

Subjects and Methods

The prospective case-control study was conducted in two villages of Matiari district in rural Sindh Pakistan from July to December 2013 and comprised healthy adult male smokers and an equal number of matching non-smokers as controls. The two villages were selected on the grounds that majority of the male inhabitants of one village were either active or passive smokers whereas it was the opposite in the other village. The size of population in our study was initially 125 and with a 0.5 margin of error the needed sample size calculated by using simplified formula for proportions14 was 95.24.

Both the cases and the controls were randomly recruited from respective villages and were matched for age height weight and socioeconomic background. Individuals suffering from any inflammatory diseases or using any medication were excluded. Healthy smokers were defined as those who were smoking 10 or more cigarettes per day for three or more years without indication of the exclusion criteria The non-smokers were those who had never smoked. CBC along with serum CRP and magnesium levels in all the subjects were measured to assess the effect of smoking on these parameters. Before blood sampling informed written consent was obtained from each subject while the approval was obtained from the Ethical Committee of Isra University Hyderabad. CBC was analysed using Sysmex Haematology Analyser while serum CRP and magnesium levels were determined by standard methods using Nyocard and Diasys kits respectively.

Data was presented as mean standard deviation (SD). Comparison of mean values between the cases and the controls was done using student's t- test. Differences were considered significant at pless than 0.001.

Results

Of the total 96 subjects in the study 48(50%) each represented the two groups. The overall age range was 20-40 years. CBC values for each group were comparable except for neutrophils which were significantly higher (pless than 0.001) and lymphocytes which were significantly raised (pless than 0.001) in smokers than in non-smokers (Table-1).

Table-1: Blood CP values compared between healthy adult male smokers and non-smokers.

Blood CP###Smokers (n=48)###Non-Smokers (n=48)###P value

Hb g/dl###12.89 2.09###12.70 2.17###0.400

HCT %###40.34 5.94###41.49 4.78###0.342

MCV fl###83.05 9.27###83.07 11.82###0.226

MCH pg###26.55 4.23###25.37 5.09###0.137

MCHC g/dl###31.94 2.19###30.50 2.65###0.315

WBC %###8.38 2.45###8.90 2.62###0.294

Neutrophils %###45.74 1.14###56.65 9.80###less than 0.001

Lymphocytes %###48.39 1.30###31.10 7.14###less than 0.001

Platelets x103/l###273.73 9.01###322.85 9.52###0.223

Table-2: Comparison of serum C-reactive protein and magnesium levels between healthy adult male smokers and non-smokers.

Serum variable###Smokers (n=48)###Non-Smokers (n=48)###P value

C- reactive protein

Normal range

(less than 5mg/L)###14.620.16###4.810.16###less than 0.001

Magnesium

Normal range

(1.8-2.6mg/dl)###1.090.38###2.520.18###less than 0.001

The mean serum CRP concentration in smokers (14.620.16mg/L) was significantly higher (pless than 0.001) compared to non-smokers (4.810.38mg/L). The mean serum magnesium concentration in non-smokers (2.520.18mg/L) compared to smokers (1.090.38mg/L) was significantly higher (pless than 0.001) (Table-2).

Serum magnesium level in all smokers was found to be less than 1.8mg/dl while serum CRP level higher than 10mg/l. On the contrary all non-smokers had their serum magnesium concentration greater than 2.0mg/dl and serum CRP less than 7.5 mg/L.

The mean CRP-to-magnesium ratio in serum samples of the smokers was 1.631.02 against 0.200.04 in non- smokers (pless than 0.001).

Discussion

The finding of the present study that male smokers had significantly higher lymphocytes count and lower counts of neutrophils compared to healthy adult male non- smokers is in full agreement with earlier findings.15

In the present study it was noted that serum magnesium levels were significantly decreased in smokers. This could be due to increased demand for magnesium by smokers owing to increased release of adrenaline and thermogenic effect of nicotine which is the main constituent of tobacco. Also smokers may get less magnesium than non- smokers because they tend to eat less.

A study7 reported raised levels of serum CRP in smokers. This is supported by the finding of the present study.

A study carried out on magnesium-deficient rats16 observed that inflammatory response was an early outcome of magnesium deficiency in rats. This is supported by the notion that magnesium inhibits inflammation and decreases CRP levels in chronic diseases.17

According to a study18 which is supported by a meta- analysis and systemic review17 individuals with less intake of magnesium were more likely to have had raised serum CRP. The independent relationship between the low serum magnesium levels and raised CRP concentrations in smokers in the present study as well as by an earlier one5 in non-diabetic non-hypertensive obese subjects suggest that hypomagnesaemia might be responsible for the activated state of immune cells. In order to clarify the role of plasma magnesium deficiency in the aetiology of the inflammatory processes interventional prospective studies are needed to be carried out to examine the effects of magnesium supplementation on serum magnesium and CRP levels in healthy adult male smokers.

The difference in the mean CRP-to-magnesium ratio in the two groups in the current study clearly indicates that smoking increased inflammation (manifested by raised levels of CRP in serum and decreased serum magnesium concentration in the smokers. However it will be interesting to see whether the ratio could be used to examine additive effects of smoking on severity and prognosis of diseases with inflammatory component.

Conclusion

Smoking significantly decreased serum magnesium concentration and increased serum CRP concentration resulting in an inverse relationship between the two in healthy adult male smokers.

Acknowledgment

We are grateful to the authorities of Isra University for financial assistance.

References

1. Das SK. Harmful health effects of cigarette smoking. Mol Cell Biochem 2003; 253: 159-65.

2. Wannamethee SG Lowe GD Shaper AG Rumley A Lennon L Whincup PH. Associations between cigarette smoking pipe/ cigar smoking and smoking cessation and haemostatic and inflammatory markers for cardiovascular disease. Eur Heart J 2005; 26: 1765 -73.

3. Libby P. Inflammation in atherosclerosis. Nature 2002; 420: 868-74.

4. Lao XQ Jiang CQ Zhang WS Adab P Lam TH Cheng KK et al. Smoking smoking cessation and inflammatory markers in older Chinese men-The Guangzhou Biobank Cohort Study. Atherosclerosis 2009; 203: 304-10.

5. Lau DC Dhillon B Yan H Szmitko PE Verma S. Adipokines: molecular links between obesity and atherosclerosis. Am J Physiol Heart Circ Physiol 2005; 288: H2031-41.

6. Pepys M Hirschfield GM. C-reactive protein: a critical update. J Clin Invest 2003; 111: 1805-12.

7. Yamada S Gotoh T Nakashima Y Kayaba K Ishikawa S Nago N et al. Distribution of serum C-reactive protein and its association with atherosclerotic risk factors in a Japanese population- Jichi Medical School Cohort Study. Am J Epidemiol 2001; 153: 1183-90.

8. Lopez-Garcia E Schulze MB Meigs JB Manson JE Rifai N Stampfer MJ et al. Consumption of trans fatty acids is related to plasma biomarkers of inflammation and endothelial dysfunction. J.Nutr 2005; 135: 562-6.

9. Dehghan A Kardys I Demaat MP Uitterlinden AG Sijbrands EJ Bootsma AH et al. Genetic variation C-reactive protein levels and incidence of diabetes. Diabetes 2007; 56: 872-8.

10. Volpe SL. Magnesium the metabolic syndrome insulin resistance and type 2 diabetes mellitus. Crit Rev Food Sci Nutr 2008; 48: 293- 300.

11. Talukder MAH Johnson WM Varadharaj S Lian J Kearns PN EL- Mahdy MA et al. Chronic cigarette smoking causes hypertension increased oxidative stress impaired NO bioavailability endothelial dysfunction and cardiac remodeling in mice. Am J Physiol Heart Circ Physiol 2011; 300: H 388-96.

12. Guerrero-Romero F Rodriguez-Moran M. Relationship between serum magnesium levels and C-reactive protein concentration in non-diabetic non-hypertensive obese subjects. Int J Obes Relat Metab Disord 2002; 26: 469-74.

13. King DE Egan BM Mainous AG Geesey ME. Elevation of C-reactive protein in people with prehypertension". J Clin Hypertension 2004; 6: 562-8.

14. Taro Y. Statistics: An introductory analysis. 2nd ed. New York: Harper and Row; 1967.

15. Lyer RA Joshi AR Esmaeil H. Effect of cigarette smoking on leukocytes count in human adult males. Int J Physiol 2014; 2: 107- 11.

16. Malpuech-BrugACopyrightre C Nowacki W Daveau M Gueux E Linard C Rock E et al. Inflammatory response following acute magnesium deficiency in the rat. Biochim Biophys Acta 2000; 1501: 91-8.

17. Dibaba DT Xun P He K. Dietary magnesium intake is inversely associated with serum C-reactive protein levels: meta-analysis and systematic review. European Journal Clinical Nutrition 2014; 68: 510-6.

18. King DE Mainous AG III Geesey ME and Woolson RF. Dietary Magnesium and C-reactive protein levels. J. Am Col of Nutr 2005; 24: 166-71.
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Publication:Journal of Pakistan Medical Association
Article Type:Clinical report
Date:Jan 31, 2015
Words:2063
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