Evaluation of the Antioxidant Capacities and Cytotoxic Effects of Ten Parmeliaceae Lichen Species.
Lichens are generally defined as symbiotic organisms resulting from the successful association between a fungus (the mycobiont) and an extracellularly located photosynthetic partner (the photobiont), which in most cases is represented by green algae, but it can also be replaced by cyanobacteria (10% of lichen symbiosis) or by the simultaneous association of both algae and cyanobacteria (3-4%) . The number of lichen species described so far (more than 27000) varies depending on authors criteria and is in continuous change due to the inclusion of molecular data . They are all characterized by their capacity to survive in the most adverse and diverse geoclimatic circumstances; actually, lichens are found disseminated from the poles to the tropics and from the highest mountains to the plains on earth and substrates, which is mainly favored by their evolved strategy of poikilohydry .
Most of the lichenized fungi belong to the phylum Ascomycota (98% of lichen species) and, among them, Parmeliaceae (Ascomycota, Lecanorales) represents the largest family of lichens. This family is widely distributed in different latitudes of both Northern and Southern hemispheres and is remarkably the best studied from systematic and phylogenetic perspectives . Lichen species included in Parmeliaceae (ca. 2700 species grouped in 80 genera) are supposedly around the 10% of total lichen species and they are characterized by having cupulate exciple, foliose, dorsiventral, and rhizinate lower surface, fruticose to subfruticose threadlike thallus, and a gray, yellow-green, and brown to olive-brown upper surface. Parmeliaceae includes several commonly known groups of lichens such as the Iceland moss (Cetraria islandica), beard-lichens (Usnea sp.), and the Oakmoss (Evernia prunastri) .
Those and other species of lichens have been used throughout ages with various purposes, in particular as dyes, perfumes, bioindicators of air pollutants, and medicinal remedies in folk medicines. As examples of therapeutic uses, Usnea barbata was used to treat hair-related diseases, Parmelia sulcata for cranial maladies, and Parmelia saxatilis for the treatment of epilepsy . However, in comparison to other natural products, biological activities of lichens are poorly known and its thorough research is mainly being developed in the last two decades; Parmeliaceae arises as the family with the highest pharmacological potential . In general, the pharmacological interest of lichens relies on the capacity of the mycobiont to produce secondary metabolites, which differ from those found in nonlichenized fungi. These unique compounds normally play an adaptive role in the symbiosis, with functions including the regulation of cell division of photobionts, allelopathy, antiherbivory, chelation of heavy metals, and light screening . Besides, lichen metabolites and especially the polymalonyl derived
polyketides (such as depsides and depsidones) have been found to exhibit manifold biological activities with potential application in human pharmacology [9,10]. Various scientific reports suggested that lichens present antimicrobial, antiprotozoal, anti-inflammatory, antipyretic, and antiproliferative (antitumor) activities [11-13]. Regarding their antioxidant potential, it could be considered that lichens are poorly known when compared to higher plants or other fungus, despite recent investigations are dealing with the issue; only a few lichens species and compounds have exerted promising antioxidative potentials (as reviewed in Fernandez-Moriano et al. ) and further studies are encouraged to deeply understand the value of lichen compounds as protective antioxidant agents.
Antioxidants comprise a heterogeneous group of compounds that share common actions in the oxidation process, such as stopping, retarding, or preventing the effects mediated by the reactive species derived from oxygen (ROS) or nitrogen (RSN) towards oxidizable substrates in biological systems. Antioxidants are especially relevant if one considers that numerous physiological and pathological processes in the human cells produce free radicals and reactive species. What is more, their overproduction leads to a situation of cellular oxidative stress, in which the endogenous antioxidant systems cannot overcome the damaging effect of ROS such as superoxide anion ([O.sup.2-]), hydrogen peroxide ([H.sub.2][O.sub.2]), hydroxyl radical (H[O.sup.*]), and singlet oxygen ([sup.1] [O.sub.2]) . Therefore, oxidative stress involves the damage to biomolecules (DNA, membrane lipids, and enzymes, among others) and is eventually a pathogenic feature of chronic diseases, including cancer and age-related neurodegenerative diseases [16, 17]. It takes special relevance within the brain cells, as they are extremely susceptible to oxidative stress and ROS due to their major consumption of oxygen and the higher content of polyunsaturated fatty acid (prone to peroxidation), among other reasons .
Many authors agree with the idea that one of the most efficient ways to counteract oxidative stress-mediated cytotoxicity is through exogenous antioxidant supplementation; antioxidant compounds may act through different mechanisms including scavenging of ROS, induction of endogenous antioxidant enzymes, and chelation of excess catalytic metals (iron, copper). Since several synthetic antioxidants have demonstrated toxicity in humans (e.g., BHA, BHT), consistence evidences support the research and use of antioxidant compounds of natural origin with that aim. What is more, many natural antioxidants, such as flavonoids, resveratrol, and other polyphenols, have been proposed as potential therapeutic tools for the prevention or delay of neurodegenerative diseases (e.g., Alzheimer's and Parkinson's disease), which currently lack effective treatments [19-21]. Consequently, in a context of growing interest towards the finding of antioxidant compounds from plant resources without any undesirable effect, in the last few years lichens emerge as an attractive field of research.
In view of this information and as part of our present research, herein we report a screening on the antioxidant capacity and phenolic profile of the methanol extract of ten Parmeliaceae lichen species from diverse locations. Out of all lichen species under investigation, some of the selected species have already been investigated for antimicrobial or enzyme inhibitory activities [22, 23], but little is known about their antioxidant capacities. In addition, as an approach to their anticancer potential, we aimed to determine their cytotoxic effects on two human cancer cell lines (the hepatocellular carcinoma-derived HepG2 and the breast adenocarcinoma MCF-7 cell line).
2. Materials and Methods
2.1. Chemicals. RPMI 1640 medium, fetal bovine serum (FBS), phosphate-buffered saline (PBS), and gentamicin were obtained from Gibco (Invitrogen, Paisley, UK). 3-(4,5-Dimethyl-2-thiazolyl)-2,5- diphenyltetrazolium bromide (MTT) and dimethyl sulphoxide (DMSO), 1,1-diphenyl-2-picrylhydrazyl (DPPH), 2,2'-azobis(2-methylpropionamidine)-dihydrochloride (AAPH), fluorescein disodium salt, and 2,4,6-Tris (2-pyridyl)-1,3,5-triazine (TPTZ) were provided by Sigma-Aldrich (St. Louis, MO, USA).
2.2. Lichen Samples. The investigated lichen samples were collected, identified, and authenticated by a taxonomist and the voucher specimens are preserved in the lichen section of MAF herbarium, Faculty of Pharmacy, Universidad Complutense de Madrid, Madrid, Spain (MAF-Lich). Their identifying data are as follows:
(i) Bulbothrix setschwanensis (Zahlbr.) Hale, Uttarakhand, Uttarkashi district, India, November 2012, MAF-LICH 20660.
(ii) Flavoparmelia caperata (L.) Hale, Gran Canaria, Canary Islands, Spain, June 2009, MAF-LICH 20662.
(iii) Flavoparmelia euplecta (Stirt.) Hale, New South Wales, Australia, February 2004, MAF-LICH 15375.
(iv) Flavoparmelia haysomii (CW Dodge) Hale, Canberra, Australia, September 1999, MAF-LICH 7535.
(v) Hypotrachyna cirrhata (Fr.) Hale, Uttarakhand, Uttarkashi district, Kedarkantha, India, November 2012, MAF-LICH 20659.
(vi) Lethariella canariensis (Ach.) Krog, Madeira, Portugal, September 2012, MAF-LICH 20663.
(vii) Myelochroa irrugans (Nyl.) Elix & Hale, Chichibu city, Nakatsugawa, Prefecture Saitama, Province Musashi, Honshu, Japan, February 2009, MAF-LICH 303.
(viii) Parmelia omphalodes (L.) Ach, Candeleda, Castilla y Leon, Spain, July 2015, MAF-LICH 20661.
(ix) Usnea aurantiacoatra (Jacq.) Bory, Navarino, La Bandera, Chile, January 2008, MAF-LICH 15686.
(x) Usnea contexta Motyka, Navarino, La Bandera, Chile, January 2005, MAF-LICH 15710.
2.3. Lichen Extracts Preparation. Drythalli of the investigated lichens (50 mg) were extracted in methanol (2 ml) for 2 h, by the extraction method of shaking maceration. Every 30 min, flasks were shaken in vortex for 1 min. The extraction was performed at room temperature (20-22[degrees]C). Afterwards, extracts were filtered (through nylon filters of 0.45 [micro]m pore) and then evaporated to dryness at room temperature . The dry residues were then weighted and kept at 4[degrees]C. Finally, extraction yields were calculated as the percentage of air-dried weight lichens/weight of the original thallus sample.
2.4. Antioxidant Activities
2.4.1. ORAC Assay. The ORAC assay was performed as previously described by Dovalos et al. . Trolox was used as the antioxidant reference compound. The samples were dissolved at a concentration of 1 mg/ml in methanol and then progressively diluted so that the concentrations in the wells ranged from 10 and 500 [micro]g/ml. Lichen extract solutions were incubated with fluorescein (70 nM) for 10 min at 37[degrees]C in 96-well plates. After incubation, AAPH (12 mM) was added and fluorescence was recorded for 98 min at excitation and emission wavelengths of 485 nm and emission of 520 nm, respectively, in a FLUOstar Optima fluorimeter (BMG Labtech, Ortenberg, Germany). Results are expressed as [micro]mol Trolox equivalents (TE)/mg sample.
2.4.2. DPPH Assay. The DPPH assay was done according to the method described by Amarowicz et al.  with some modifications. Lichen extracts solutions were incubated with DPPH (50 [micro]m) for 30 min at dark in 96-well plates. Absorbance was then measured at 517 nm in a FLUOstar Optima fluorimeter (BMG Labtech, Ortenberg, Germany). Trolox was used as antioxidant reference compound. Results are expressed as E[C.sub.50] value (effective concentration in [micro]g/ml).
2.4.3. FRAP Assay. The ferric reducing antioxidant activity of lichen extracts was measured using FRAP assay by the method described Sonchez-Muniz et al. . The working FRAP reagent was prepared by mixing TPTZ (2,4,6-tri(2-pyridyl)-s-triazine), ferric chloride, and buffer solution. Lichen extracts (1mg/ml) were then mixed with working FRAP reagent and incubated for 30 min at 37[degrees]C. Absorbance was read at 595 nm in a Spectrostar Nanomicroplate reader (BMG Labtech Inc., Ortenberg, Germany). The values are expressed as [micro]mol [Fe.sup.2+]/g extract.
2.5. Determination of Total Phenolic Content. The total phenolic content in lichen extracts was measured using the Folin-Ciocalteu assay . Briefly, lichen extract at 1mg/ml in methanol (0.5 ml) was added to test tubes and mixed with Folin-Ciocalteu reagent (0.5 ml), [Na.sub.2]C[O.sub.3] solution (75 g/l; 10 ml), and distilled water (14 ml). This reaction mixture was then incubated at dark for 1 h. Absorbance was measured at 760 nm using a spectrophotometer (Uvikon930, Kontron Instruments, Bardsey, UK) and compared to a gallic acid calibration curve. Results are expressed as [micro]g gallic acid equivalent/mg dry extract.
2.6. Cancer Cell Lines. MCF-7 human breast cancer cells and HepG2 human liver cancer lines were obtained from the NCI-Frederick Cancer DCTD Tumor/Cell line Repository (Frederick National Laboratory for Cancer Research, National Cancer Institute). MCF-7 and HepG2 cells were cultured in RPMI 1640 medium, supplemented with FBS (10%), and gentamicin (0.5%) at 37[degrees]C in a humidified 5% C[O.sub.2] incubator.
2.7. Cell Viability Assay. Cell viability was determined by using the colorimetric MTT metabolic activity assay. Cells were treated with different concentrations of lichen extracts from 5 [micro]g/ml to 800 [micro]g/ml for 24 h. After cell treatments, MTT solution (2 mg/ml) was added and incubated for 1 h at 37[degrees]C. Following, MTT was removed and the formazan crystals were dissolved in DMSO. The absorbance was read at 550 nm in a Spectrostar Nanomicroplate reader (BMG Labtech Inc., Ortenberg, Germany) and results were expressed as percentage of cell viability (control absorbance values were taken as 100%) .
2.8. Statistical Analysis. Data were analyzed by one-way analysis of variance (ANOVA) followed by Tukey's test (p < 0.05) for multiple comparisons using Statgraphics Centurion XVI software. Data of all assays in the current work correspond to biologically independent experiments performed in triplicate, and the mean values are shown.
Pearson's correlation coefficient (.R)has been used to measure the strength and direction of a linear correlation between phenolic content and the different antioxidant activities (in ORAC, DPPH and FRAP assays). Correlations were also calculated using the Statgraphics software. The correlation coefficients are significant at the 0.05 level.
3. Results and Discussion
3.1. Morphoanatomical Features of the Identified Parmeliaceae Lichens. The illustrations of the thallus structures of the ten lichen species from Parmeliaceae family investigated in the present study are shown in Figure 1. The selection of these species was based on a geographic distribution pattern, with samples from 4 different continents (Oceania, Asia, Europe, including the Canary Islands, and America), on the presence of diverse extrolites contained in these species and focused on the phytochemistry, pharmacological potential, and phylogenetic features of Parmeliaceae (see ).
3.2. Extraction Yields and Total Phenolic Content. The results for extraction yields and the content in phenolic compounds of the methanol extracts of the studied species are summarized in Table 1. Yield values ranged between 2.17 and 14.31% w/w. The highest yield was observed for Myelochroa irrugans methanol extract, whereas Usnea aurantiacoatra methanol extract showed the lowest yield. These extraction yields were similar to those previously reported for the methanol extracts of other Parmeliaceae species [24, 30].
Lichens synthesize unique secondary metabolites, especially depsides, depsidones, and dibenzofuran derivatives with phenol groups in their structure and mainly through the acetyl polymalonyl biosynthetic pathway . Phenolic compounds possess remarkable antioxidant capabilities through free radical scavenging activity, metal ion-chelating action, and modulation of cytoprotective enzymes activity . The total phenolic content was determined by Folin-Ciocalteu method and using a standard curve of gallic acid (0-400 [micro]g/ml). Flavoparmelia euplecta showed the highest phenolic content (101.4 [micro]g GA/mg), followed by Myelochroa irrugans (92.5 [micro]g GA/mg) and Parmelia omphalodes (65.0 [micro]g GA/mg). The lowest phenolic content was found for Usnea contexta (20.7 [micro]g GA/mg) and Usnea aurantiacoatra (22.4 [micro]g GA/mg). In general, since methanol is used as an efficient solvent for the extraction of phenolic compounds, there is a good correlation between the yield of the maceration process and the content of phenolic compounds . Our results are in line with this: Usnea sp. (U. contexta and U. auranticoatra) showed the lowest yield and phenolic content, whereas Flavoparmelia euplecta and Myelochroa irrugans were two of the species showing the highest extraction yields.
3.3. Antioxidant Capacities. Antioxidant capacities of the ten Parmeliaceae species were determined by evaluating the free radical scavenging activities (ORAC and DPPH assays) and the ferric reducing power (FRAP assay) of their methanol extracts. Results are shown in Table 1.
Through the ORAC assay, we aimed to determine the capacity of lichen extracts to scavenge peroxyl radicals in vitro. The ORAC values obtained showed the highest scavenging capacity for Flavoparmelia euplecta (3.30 [micro]mol TE/mg dry extract) followed by Parmelia omphalodes (3.15 [micro]mol TE/mg dry extract), Flavoparmelia caperata (2.81 [micro]mol TE/mg dry extract), and Myelochroa irrugans (2.64 [micro]mol TE/mg dry extract). In this assay, the lowest scavenging action was shown for Usnea aurantiacoatra, with an ORAC value of 0.32 [micro]mol TE/mg dry extract.
With regard to DPPH method, Myelochroa irrugans (E[C.sub.50] = 384 [micro]g/ml) and Flavoparmelia euplecta (E[C.sub.50] = 582 [micro]g/ml) presented the strongest DPPH radical scavenging activity. On the other hand, the lowest free radical scavenging effectiveness was displayed by the methanol extract of Flavoparmelia caperata (E[C.sub.50] = 3216 [micro]g/ml) and Lethariella canariensis (E[C.sub.50] = 2894 [micro]g/ml).
Both ORAC and DPPH assays measure the radical scavenging ability of test samples to neutralize the reactive and oxidative action of free radicals. However, the fundament regarding the mechanisms of antioxidation is different: whereas ORAC method evaluates the antioxidant ability based on the hydrogen atom transference capacity (HAT mechanism), the reaction mechanism of DPPH test proceeds via single electron transfer (ET). These different mechanisms of quenching radicals along the experiments may explain the distinct behaviors of the methanol extracts of the studied Parmeliaceae lichens .
FRAP assay measures the reducing power of samples via direct electron donation and the reduction of ferric tripyridyltriazine [[Fe.sup.3+]-TPTZ] complex to ferrous tripyridyltriazine [[Fe.sup.2+]-TPTZ]. Among the ten methanol extracts studied, the ferric reducing antioxidant power was the highest for Hypotrachyna cirrhata (316 [micro]mol of [Fe.sup.2+] eq/g sample), Flavoparmelia euplecta (273 [micro]mol of [Fe.sup.2+] eq/g sample), and Myelochroa irrugans (266 [micro]mol of [Fe.sup.2+] eq/g sample). In the contrary, Usnea aurantiacoatra methanol extract possessed the lowest reducing ferric capacity (98 [micro]mol of [Fe.sup.2+] eq/g sample).
3.4. Correlation between Total Phenolic Content and Antioxidant Capacities. Since lichens contain diverse phenolic constituents and these kinds ofcompounds are known to possess antioxidant properties, correlations between each antioxidant assay (ORAC, DPPH and FRAP) and total phenolic content were investigated by a regression analysis (correlation coefficient, R).
As shown in Figure 2, there is a moderate to high positive correlation between the antioxidant parameters measured and the amount of phenolic compounds present in the different extracts. Actually, the correlations were very similar for all assays, with the following R values versus total phenolic content: R = 0.743 * for ORAC assay ([R.sup.2] = 0.552); R = 0.746 * for DPPH assay ([R.sup.2] = 0.556); and R = 0.706 * for FRAP method ([R.sup.2] = 0.498) (* stands for statistical significance of Pearson's correlation coefficient at p < 0.05).
Although slightly higher, ORAC and DPPH radical scavenging capacities presented a better correlation with phenolic content than ferric reducing activity. In general, we found that the lichen extracts showing the highest phenolic content (e.g., Flavoparmelia euplecta and Myelochroa irrugans) were among the most active ones in the chemical tests evaluating antioxidant potential via radical scavenging mechanism; also, those with the lowest contents in polyphenols (Usnea auranticoatra and Usnea contexta) displayed the weakest antioxidant activities. However, we found moderate variations in the effect displayed by other species with intermediate phenolic content.
The lack of a higher correlation between the antioxidant capacities of lichen methanol extracts and the content in polyphenols might be explained by several facts including the structural diversity of phenolic compounds. Previous studies on structure-activity relationship have demonstrated that antioxidant potency and reaction mechanisms of phenol derivatives compounds depend on both hydroxyl group position and number. Lopes et al.  confirmed that hydrogen bonding of carbonyl groups to hydroxyl radicals reduced significantly the scavenging activity of lichen constituents. In higher plants, there is scientific consensus on a good correlation between the total phenolic content and the antioxidant capacity of a certain sample [35, 36]. However, there are many discrepancies on such correlation in lichen species. Some authors did not find any positive correlation between antioxidant activity and total phenolic content of lichen extracts , but in contrast, others described strong relationships between total phenolic and flavonoid contents and the antioxidant effect . Our results are in line with this information and suggest that the antioxidant activity of some tested extracts might be attributable to the presence of nonphenolic compounds. For instance, apart from phenols, phytochemical analysis of lichens has revealed the presence of terpenoids in different Parmeliaceae species , and these minor compounds have demonstrated to possess antioxidant properties and therefore to contribute to the total antioxidant activity . Besides, it should be pointed out that individual phenolics may present distinct antioxidant activities in the different chemical tests, and there may be synergistic or antagonistic interactions between phenolics and other compounds like proteins, carbohydrates, and so forth.
3.5. Cytotoxic Activities. Up to date, only a few studies have evaluated the cytotoxic properties of Parmeliaceae lichens. Mitrovioc et al.  reported the antiproliferative activity of the methanol extracts of some Parmeliaceae species different to these investigated in the present study (such as Parmelia sulcata, Flavoparmelia caperata, Evernia prunastri, and Hypogymnia physodes) against the colon cancer adenocarcinoma cell line HCT-116. Moreover, Bezivin et al.  studied the anticancer activity of eight extracts obtained from Parmeliaceae spp. collected in various places of Brittany, among which Parmelia caperata, Parmelia perlata, and Usnea rubicunda are found, on human and murine cancer lines. However, to our knowledge, the species included in our study has not been previously evaluated against the MCF-7 and HepG2 cell lines.
Therefore, the cytotoxic effects of the methanol extracts of the ten studied Parmeliaceae species were evaluated, as an approach to their anticancer potential, against the human-derived MCF-7 (human breast adenocarcinoma) and HepG2 (human hepatocellular carcinoma) cancer cell lines. Their effects on cell viability were analyzed and quantified by using MTT assay after 24-hour treatment with a range of concentrations of extracts from 5 to 800 [micro]g/mL. Through this assay, cell viability is determined based on the measurement of mitochondrial function, as MTT is transformed into formazan crystals in living cells in which mitochondrial dehydrogenases are functional. As shown in Figure 3, cell viability decreased in a concentration-dependent manner in both MCF-7 and HepG2 cell lines for all assayed lichen extracts. The lethal doses 50% (L[D.sub.50]) were determined and these data are shown in Table 2. Differences among cancer cell lines types in their sensitivity to tested methanol lichen extracts were observed.
The lichen extracts that possessed stronger cytotoxic activity towards MCF-7 human breast cancer cells were Lethariella canariensis (L[D.sub.50] = 66 [micro]g/mL) followed by Flavoparmelia euplecta (L[D.sub.50] = 67 [micro]g/mL) and Bulbothrix setschwanensis (L[D.sub.50] = 91 [micro]g/mL). In contrast, Usnea aurantiacoatra, Hypotrachyna cirrhata, and Flavoparmelia haysomii exhibited very low toxicity with L[D.sub.50] values of 339 [micro]g/mL, 281 [micro]g/mL, and 212 [micro]g/mL, respectively. The screening for cytotoxicity against HepG2 human hepatocarcinoma cells revealed that Myelochroa irrugans reduced significantly their cell viability with L[D.sub.50] of 22 [micro]g/mL; methanol extracts of the Parmeliaceae species Hypotrachyna cirrhata (L[D.sub.50] = 41 [micro]g/mL) and Usnea contexta (L[D.sub.50] = 54 [micro]g/mL) induced also remarkable toxicity against HepG2 cells. On the other hand, the less active lichen extracts were Lethariella canariensis and Flavoparmelia caperata which showed values of L[D.sub.50] of 351 [micro]g/mL and 272 [micro]g/mL, respectively. According to the National Cancer Institute from USA, crude extracts are considered to possess significant cytotoxic activity when I[C.sub.50] < 30 [micro]g/mL. The methanol extract of Myelochroa irrugans falls within this criteria, being of interest as anticancer agent against liver cancer.
Currently there is growing evidence that supports the involvement of reactive oxygen species in the etiology of fat-related neoplasms such as cancer of the breast, colorectum, and liver due to the peroxidation of lipids and subsequent formation of toxic aldehydes . With this regard, antioxidant phenolic compounds may counteract the carcinogenic potential of ROS via scavenging of free radicals and blockage of peroxidation chain reaction. Actually, positive correlation between phenolic content and cytotoxic activities against human cancer cell lines has been found in vitro [44, 45]. This correlation may explain the interesting cytotoxic effects displayed by the methanol extracts of Myelochroa irrugans and Flavoparmelia euplecta.
Based on the overall results for antioxidant and cytotoxic activities, the methanol extracts of the three species that exerted the most promising pharmacological potential were Flavoparmelia euplecta, Myelochroa irrugans, and Parmelia omphalodes. These three species belong to the major phylogenetic clade in the Parmeliaceae family: the parmelioid clade. Within the parmelioid clade, Flavoparmelia is grouped in the parmotremoid clade whereas Myelochroa and Parmelia belong to the Parmelina and Parmelia clades, respectively .
For these lichen species, there is not any previous study that has evaluated their antioxidant or their cytotoxic/anticancer actions. The biological properties demonstrated herein can be attributed to the presence of bioactive secondary metabolites in the extracts. Flavoparmelia euplecta has been reported to contain the dibenzofuran usnic acid and the depsidone protocetraric acid ; on the other hand, Myelochroa irrugans present as bioactive compounds the depside atranorin, the triterpenes zeorin and leucotylic acid, and the xanthone secalonic acid A ; and the main secondary metabolites in Parmelia omphalodes have been described to be the depsides atranorin, the depsidones salazinic and lobaric acids, and the acyclic fatty acid protolichesterinic acid .
There are some previous studies that have investigated both the antioxidant and the cytotoxic activities of some of these lichen substances, and their results may help to explain our findings. Usnic acid has been one of the most studied lichen compound for its pharmacotoxicological actions, including its antioxidant potential. This dibenzofuran has shown to increase the levels of superoxide dismutase (SOD), glutathione peroxidase (GPx), and reduced glutathione (GSH) and to reduce lipid peroxidation on indomethacin-induced gastric ulcer in rats . In another study, usnic acid and also salazinic acid (although to a lesser extent) have demonstrated protecting astrocytes against hydrogen peroxide-induced oxidative stress by increasing cell viability and inhibiting intracellular ROS production; this protection seems to be related, at least in part, to its peroxyl scavenger properties . Moreover, usnic acid was also effective as an antioxidant agent against lipopolysaccharide-induced lung injury through the decrease of the levels of hydrogen peroxide, myeloperoxidase, and malondialdehyde (MDA), and the increase of the levels of superoxide dismutase (SOD) and reduced glutathione (GSH) . Atranorin has also shown to possess remarkable antioxidant activity by inhibiting lipid peroxidation  and acting as a superoxide  and free radical diphenyl picryl hydrazyl (DPP[H.sup.*]) scavenger . Manojlovic et al.,  evidenced that salazinic acid has stronger superoxide anion radical scavenging activity and reducing power than protocetraric acid.
With respect to cytotoxic/anticancer activities, the isolated compounds protocetraric acid and usnic acid have demonstrated to significantly inhibit the growth of the melanoma UACC-62 and B16-F10 cell lines . In another study, the compounds salazinic acid and protocetraric acid were proved to exert high cytotoxic activity against FemX (human melanoma) and LS174 (human colon carcinoma) . Moreover, atranorin and usnic acid are potent anticancer agents in both the A2780 human ovarian cancer cell line and the HT-29 human colon cancer cell line, acting through a mitochondrial pathway . Furthermore, the compound protolichesterinic acid has resulted to be a promising agent against the cervix adenocarcinoma HeLa cell line via activation of caspase pathway .
Still, further studies are required to better understand the antioxidant and cytotoxic potentials of the lichen species studied here and their active metabolites. It would be of interest to isolate the main bioactive compounds found in Flavoparmelia euplecta, Myelochroa irrugans, and Parmelia omphalodes and to determine which compound contributes in a higher degree to the antioxidant activity and cytotoxic action against MCF-7 and HepG2 cell lines.
Taken together, our results suggest that the Parmeliaceae lichen species Flavoparmelia euplecta and Myelochroa irrugans, which showed the highest antioxidant and cytotoxic actions, may arise as promising sources of natural compounds with pharmacological interest. Further research is encouraged to determine the real potential of their secondary metabolites in the therapy of oxidative stress-related diseases and cancer.
The authors declare that there is no conflict of interests regarding the publication of this paper.
C. Fernandez-Moriano and E. Gonzalez-Burgos contributed equally to this work.
This work was supported by the research project of the Spanish Ministry of Economy [Ref. CGL2013-42498] awarded to Professor Dr. A. Crespo and Professor Dr. P. K. Divakar and the predoctoral grant awarded by the Spanish Ministry of Education, Culture and Sports to Carlos Fernandez-Moriano [no. FPU12/03824].
 D. L. Hawksworth and R. Honegger, "The lichen thallus: a symbiotic phenotype of nutritionally specialized fungi and its response to gall producers," in Plant Galls: Organisms, Interactions, Populations, M. A. J. Williams, Ed., pp. 77-98, Clarendon Press, Oxford, UK, 1994.
 M. C. Molina, R. Del-Prado, P. K. Divakar, D. Saonchez-Mata, and A. Crespo, "Another example of cryptic diversity in lichen-forming fungi: the new species Parmelia mayi (Ascomycota: Parmeliaceae)," Organisms Diversity & Evolution, vol. 11, no. 5, pp. 331-342, 2011.
 R. Honegger, "The lichen symbiosis--what is so spectacular about it?" Lichenologist, vol. 30, no. 3, pp. 193-212, 1998.
 A. Thell, A. Crespo, P. K. Divakar et al., "A review of the lichen family parmeliaceae--history, phylogeny and current taxonomy," Nordic Journal of Botany, vol. 30, no. 6, pp. 641-664, 2012.
 A. Crespo, H. T. Lumbsch, J.-E. Mattsson et al., "Testing morphology-based hypotheses of phylogenetic relationships in Parmeliaceae (Ascomycota) using three ribosomal markers and the nuclear RPB1 gene," Molecular Phylogenetics and Evolution, vol. 44, no. 2, pp. 812-824, 2007.
 S. Malhotra, R. Subban, and A. Singh, "Lichens-role in traditional medicine and drug discovery," The Internet Journal of Alternative Medicine, vol. 5, no. 2, pp. 1-5, 2008.
 M. P. Gomez-Serranillos, C. Fernandez-Moriano, E. Gonzalez-Burgos, P. K. Divakar, and A. Crespo, "Parmeliaceae family: phytochemistry, pharmacological potential and phylogenetic features," RSC Advances, vol. 4, no. 103, pp. 59017-59047, 2014.
 K. Molnar and E. Farkas, "Current results on biological activities of lichen secondary metabolites: a review," Journal of Biosciences, vol. 65, no. 3-4, pp. 157-173, 2010.
 E. Stocker-Worgotter, "Metabolic diversity of lichen-forming ascomycetous fungi: culturing, polyketide and shikimate metabolite production, and PKS genes," Natural Product Reports, vol. 25, no. 1, pp. 188-200, 2008.
 V. Shukla, G. P. Joshi, and M. S. M. Rawat, "Lichens as a potential natural source of bioactive compounds: a review," Phytochemistry Reviews, vol. 9, no. 2, pp. 303-314, 2010.
 B. Rankovic, M. Misia, and S. Sukdolak, "Antimicrobial activity of extracts of the lichens Cladonia furcata, Parmelia caperata, Parmelia pertusa, Hypogymnia physodes and Umbilicaria polyphylla," British Journal of Biomedical Science, vol. 64, no. 4, pp. 143-148, 2007.
 T. Mitrovic, S. Stamenkovic, V. Cvetkovic, M. Nikolic, S. Tosic, and D. Stojicic, "Lichens as source of versatile bioactive compounds," Biologica Nyssana, vol. 2, pp. 1-6, 2011.
 M. M. Kosanic, B. R. Rankovia, and T. P. Stanojkovia, "Antioxidant, antimicrobial and anticancer activities of three Parmelia species," Journal of the Science of Food and Agriculture, vol. 92, no. 9, pp. 1909-1916, 2012.
 C. Fernandez-Moriano, M. P. Gomez-Serranillos, and A. Crespo, "Antioxidant potential of lichen species and their secondary metabolites. a systematic review," Pharmaceutical Biology, vol. 54, no. 1, pp. 1-17, 2016.
 B. Halliwell and J. M. C. Gutteridge, Free Radicals in Biology and Medicine, Clarendon Press, Oxford, UK, 4th edition, 2007.
 S. Gandhi and A. Y. Abramov, "Mechanism of oxidative stress in neurodegeneration," Oxidative medicine and cellular longevity, vol. 2012, p. 428010, 2012.
 R. Thanan, S. Oikawa, Y. Hiraku et al., "Oxidative stress and its significant roles in neurodegenerative diseases and cancer," International Journal of Molecular Sciences, vol. 16, no. 1, pp. 193-217, 2014.
 J. Friedman, "Why is the nervous system vulnerable to oxidative stress?" in Oxidative Stress and Free Radical Damage in Neurology, Oxidative Stress in Applied Basic Research and Clinical Practice, N. Gadoth and Gobel, Eds., Springer Science, Business Media, LLC, 2011.
 C. Guerra-Araiza, A. L. Alvarez-Mejia, S. Sanchez-Torres et al., "Effect of natural exogenous antioxidants on aging and on neurodegenerative diseases," Free Radical Research, vol. 47, no. 6-7, pp. 451-462, 2013.
 I. Solanki, P. Parihar, M. L. Mansuri, and M. S. Parihar, "Flavonoid-based therapies in the early management of neurodegenerative diseases," Advances in Nutrition, vol. 6, no. 1, pp. 64-72, 2015.
 E. Tellone, A. Galtieri, A. Russo, B. Giardina, and S. Ficarra, "Resveratrol: a focus on several neurodegenerative diseases," Oxidative Medicine and Cellular Longevity, vol. 2015, Article ID 392169, 14 pages, 2015.
 V. K. Gupta, M. P. Darokar, D. Saikia, A. Pal, A. Fatima, and S. P. S. Khanuja, "Antimycobacterial activity of lichens," Pharmaceutical Biology, vol. 45, no. 3, pp. 200-204, 2007.
 H. S. Anil Kumar, T. R. Prashith Kekuda, K. S. Vinayaka, D. Swathi, and T. M. Venugopal, "Anti-obesity (Pancreatic lipase inhibitory) activity of Everniastrum cirrhatum (Fr.) Hale (Parmeliaceae)," Pharmacognosy Journal, vol. 3, no. 19, pp. 65-68, 2011.
 C. Fernandez-Moriano, P. K. Divakar, A. Crespo, and M. P. Gomez-Serranillos, "Neuroprotective activity and cytotoxic potential of two parmeliaceae lichens: identification of active compounds," Phytomedicine, vol. 22, no. 9, pp. 847-855, 2015.
 A. Davalos, C. Gamez-Cordovas, and B. Bartoloma, "Extending applicability of the oxygen radical absorbance capacity (ORAC-fluorescein) assay," Journal of Agricultural and Food Chemistry, vol. 52, no. 1, pp. 48-54, 2004.
 R. Amarowicz, R. B. Pegg, P. Rahimi-Moghaddam, B. Barl, and J. A. Weil, "Free-radical scavenging capacity and antioxidant activity of selected plant species from the Canadian prairies," Food Chemistry, vol. 84, no. 4, pp. 551-562, 2004.
 F. J. Sanchez-Muniz, R. Olivero-David, M. Triki et al., "Antioxidant activity of Hypericum perforatum L. extract in enriched n-3 PUFA pork meat systems during chilled storage," Food Research International, vol. 48, no. 2, pp. 909-915, 2012.
 O. Folin and V. Ciocalteu, "On tyrosine and tryptophane determinations in proteins," The Journal of Biological Chemistry, vol. 73, pp. 627-650, 1927.
 T. Mosmann, "Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays," Journal of Immunological Methods, vol. 65, no. 1-2, pp. 55-63, 1983.
 G. Stojanovic, I. Stojanovic, V. Stankov-Jovanovic, V. Mitia, and D. Kostiac, "Reducing power and radical scavenging activity of four Parmeliaceae species," Central European Journal of Biology, vol. 5, no. 6, pp. 808-813, 2010.
 M. Leopoldini, N. Russo, and M. Toscano, "The molecular basis of working mechanism of natural polyphenolic antioxidants," Food Chemistry, vol. 125, no. 2, pp. 288-306, 2011.
 S. E. Atawodi, J. C. Atawodi, G. A. Idakwo et al., "Evaluation of the polyphenol content and antioxidant properties of methanol extracts of the leaves, stem, and root barks of Moringa oleifera Lam," Journal of Medicinal Food, vol. 13, no. 3, pp. 710-716,2010.
 R. Apak, K. Guclu, B. Demirata et al., "Comparative evaluation of various total antioxidant capacity assays applied to phenolic compounds with the CUPRAC assay," Molecules, vol. 12, no. 7, pp. 1496-1547, 2007.
 T. I. B. Lopes, R. G. Coelho, N. C. Yoshida, and N. K. Honda, "Radical-scavenging activity of orsellinates," Chemical and Pharmaceutical Bulletin, vol. 56, no. 11, pp. 1551-1554, 2008.
 N. Turkmen, F. Sari, and Y. S. Velioglu, "Effects of extraction solvents on concentration and antioxidant activity of black and black mate tea polyphenols determined by ferrous tartrate and Folin-Ciocalteu methods," Food Chemistry, vol. 99, no. 4, pp. 835-841, 2006.
 M. Alothman, R. Bhat, and A. A. Karim, "Antioxidant capacity and phenolic content of selected tropical fruits from Malaysia, extracted with different solvents," Food Chemistry, vol. 115, no. 3, pp. 785-788, 2009.
 F. Odabasoglu, A. Aslan, A. Cakir et al., "Antioxidant activity, reducing power and total phenolic content of some lichen species," Fitoterapia, vol. 76, no. 2, pp. 216-219, 2005.
 B. R. Rankovia, M. M. Kosania, and T. P. Stanojkovic, "Antioxidant, antimicrobial and anticancer activity of the lichens Cladonia furcata, Lecanora atra and Lecanora muralis," BMC Complementary and Alternative Medicine, vol. 11, article 97, 2011.
 S. T. Abdullah, H. Hamid, M. Ali, S. H. Ansari, and M. S. Alam, "Two new terpenes from the lichen Parmelia perlata," Indian Journal of Chemistry--Section B Organic and Medicinal Chemistry, vol. 46, no. 1, pp. 173-176, 2007.
 J. Grafimann, "Terpenoids as plant antioxidants," Vitamins and Hormones, vol. 72, pp. 505-535, 2005.
 T. Mitrovio, S. Stamenkovic, V. Cvetkovio et al., "Antioxidant, antimicrobial and antiproliferative activities of five lichen species," International Journal of Molecular Sciences, vol. 12, no. 8, pp. 5428-5448, 2011.
 C. Bezivin, S. Tomasi, F. Lohezic-Le Devohat, and J. Boustie, "Cytotoxic activity of some lichen extracts on murine and human cancer cell lines," Phytomedicine, vol. 10, no. 6-7, pp. 499-503, 2003.
 R. W. Owen, A. Giacosa, W. E. Hull, R. Haubner, B. Spiegelhalder, and H. Bartsch, "The antioxidant/anticancer potential of phenolic compounds isolated from olive oil," European Journal ofCancer, vol. 36, no. 10, pp. 1235-1247, 2000.
 V. Rajkumar, G. Guha, and R. Ashok Kumar, "Antioxidant and anti-neoplastic activities of Picrorhiza kurroa extracts," Food and Chemical Toxicology, vol. 49, no. 2, pp. 363-369, 2011.
 D. Atmani, M. B. Ruiz-Larrea, J. I. Ruiz-Sanz, L. J. Lizcano, F. Bakkali, and D. Atmani, "Antioxidant potential, cytotoxic activity and phenolic content of Clematis flammula leaf extracts," Journal of Medicinal Plants Research, vol. 5, no. 4, pp. 589-598, 2011.
 P. K. Divakar, A. Crespo, M. Wedin et al., "Evolution of complex symbiotic relationships in a morphologically derived family of lichen-forming fungi," New Phytologist, vol. 208, no. 4, pp. 1217-1226, 2015.
 J. A. Elix, "Parmeliaceae," Flora of Australia, vol. 55, pp. 1-360, 1994.
 P. K. Divakar and D. K. Upreti, Parmelioid Lichens in India: A Revisionary Study, Bishen Singh Mahrendra Pal Singh, Dehradun, India, 2005.
 M. E. Hale, "A Monograph of the Lichen Genus Parmelia Acharius sensu stricto (Ascomycotina: Parmeliaceae)," Smithsonian Contributions to Botany, vol. 66, 55 pages, 1987.
 F. Odabasoglu, A. Cakir, H. Suleyman et al., "Gastroprotective and antioxidant effects of usnic acid on indomethacin-induced gastric ulcer in rats," Journal ofEthnopharmacology, vol. 103, no. 1, pp. 59-65, 2006.
 G. A. de Paz, J. Raggio, M. P. Gomez-Serranillos et al., "HPLC isolation of antioxidant constituents from Xanthoparmelia spp.," Journal of Pharmaceutical and Biomedical Analysis, vol. 53, no. 2, pp. 165-171, 2010.
 Z.-Q. Su, Z.-Z. Mo, J.-B. Liao et al., "Usnic acid protects LPS-induced acute lung injury in mice through attenuating inflammatory responses and oxidative stress," International Immunopharmacology, vol. 22, no. 2, pp. 371-378, 2014.
 F. J. Toledo Marante, A. Garcia Castellano, F. Estevez Rosas, J. Quintana Aguiar, and J. Bermejo Barrera, "Identification and quantitation of allelochemicals from the lichen Lethariella canariensis: phototoxicity and antioxidative activity," Journal of Chemical Ecology, vol. 29, no. 9, pp. 2049-2071, 2003.
 M. G. D. Melo, J. P. A. dos Santos, M. R. Serafini et al., "Redox properties and cytoprotective actions of atranorin, a lichen secondary metabolite," Toxicology in Vitro, vol. 25, no. 2, pp. 462-468, 2011.
 N. Valencia-Islas, A. Zambrano, and J. L. Rojas, "Ozone reactivity and free radical scavenging behavior of phenolic secondary metabolites in lichens exposed to chronic oxidant air pollution from Mexico City," Journal of Chemical Ecology, vol. 33, no. 8, pp. 1619-1634, 2007.
 N. Manojlovio, B. Rankovic, M. Kosanio, P. Vasiljevio, and T. Stanojkovioc, "Chemical composition of three Parmelia lichens and antioxidant, antimicrobial and cytotoxic activities of some their major metabolites," Phytomedicine, vol. 19, no. 13, pp. 1166-1172, 2012.
 L. F. G. Brandao, G. B. Alcantara, M. F. C. Matos et al., "Cytotoxic evaluation of phenolic compounds from lichens against melanoma cells," Chemical & Pharmaceutical Bulletin, vol. 61, no. 2, pp. 176-183, 2013.
 M. Backorovo, R. Jendzelovsky, M. Kello, M. Backor, J. Mikes, and P. Fedorocko, "Lichen secondary metabolites are responsible for induction of apoptosis in HT-29 and A2780 human cancer cell lines," Toxicology in Vitro, vol. 26, no. 3, pp. 462-468, 2012.
 F. Brisdelli, M. Perilli, D. Sellitri et al., "Cytotoxic activity and antioxidant capacity of purified lichen metabolites: an in vitro study," Phytotherapy Research, vol. 27, no. 3, pp. 431-437, 2013.
C. Fernandez-Moriano, (1) E. Gonzalez-Burgos, (1) P. K. Divakar, (2) A. Crespo, (2) and M. P. Gomez-Serranillos (1)
(1) Department of Pharmacology, Faculty of Pharmacy, University Complutense of Madrid, Plaza Ramon y Cajal s/n, 28040 Madrid, Spain
(2) Department of Plant Biology II, Faculty of Pharmacy, University Complutense of Madrid, Plaza Ramon y Cajal s/n, 28040 Madrid, Spain
Correspondence should be addressed to M. P. Gomez-Serranillos; email@example.com
Received 31 July 2016; Revised 28 October 2016; Accepted 8 November 2016
Academic Editor: Michal Tomczyk
Caption: Figure 1: (a) Bulbothrix setschwanensis (Zahlbr.) Hale; (b) Flavoparmelia caperata (L.) Hale; (c) Flavoparmelia euplecta (Stirt.) Hale; (d) Flavoparmelia haysomii (CW Dodge) Hale; (e) Hypotrachyna cirrhata (Fr.) Hale; (f) Lethariella canariensis (Ach.) Krog; (g) Myelochroa irrugans (Nyl.) Elix & Hale; (h) Parmelia omphalodes (L.) Ach; (i) Usnea aurantiacoatra (Jacq.) Bory; (j) Usnea contexta Motyka.
Caption: Figure 2: Correlations between each antioxidant assay (ORAC, DPPH, and FRAP) and the total phenolic content. The correlation coefficients are significant at the level of p < 0.05.
Caption: Figure 3: Cell viability of HepG2 and MCF-7 cells treated with different concentrations of Parmeliaceae lichen extracts for 24 h. Cell viability of control cells was normalized to 100%. Means values [+ or -] SD, * p < 0.05 versus control.
Table 1: Yields of extraction, antioxidant capacities (ORAC method, DPPH assay, and FRAP method), and total phenolic content of the ten methanol extracts of Parmeliaceae lichens species. Statistical significances (of antioxidant capacities values) for multiple comparisons from Tukey's test are shown in superscripts. a: statistically significant differences versus values of Bulbothrix setschwanensis; b: versus Flavoparmelia caperata; c: versus Flavoparmelia euplecta; d: versus Flavoparmelia haysomii; e: versus Hypotrachyna cirrhata; f: versus Lethariella canariensis; g: versus Myelochroa irrugans; h: versus Parmelia omphalodes; i: versus Usnea aurantiacoatra; j: versus Usnea contexta (p < 0.05). ORAC value Yield ([micro]mol TE/ Lichen species (% w/w) mg dry extract) Bulbothrix 9.83 [+ or -] 1.68 1.64 [+ or -] 0.13 setschwanensis (b,c,e,g,h,i) Flavoparmelia 11.31 [+ or -] 1.95 2.81 [+ or -] 0.33 caperata (a,c,d,e,f,i,j) Flavoparmelia 12.14 [+ or -]3.36 3.30 [+ or -] 0.24 euplecta (a,b,d,e,f,g,i,j) Flavoparmelia 14.02 [+ or -] 3.00 1.52 [+ or -] 0.08 haysomii (b,c,e,g,h,i) Hypotrachyna 9.70 [+ or -] 2.05 1.11 [+ or -] 0.07 cirrhata (a,b,c,d,g,h,i) Lethariella 5.21 [+ or -] 1.31 1.30 [+ or -] 0.07 canariensis (b,c,g,h,i) Myelochroa 14.31 [+ or -] 2.80 2.64 [+ or -] 0.25 irrugans (a,c,d,e,f,h,i,j) Parmelia 7.32 [+ or -] 1.22 3.15 [+ or -] 0.14 omphalodes (a,d,e,f,g,i,j) Usnea 2.17 [+ or -] 0.65 0.32 [+ or -] 0.03 aurantiacoatra (a,b,c,d,e,f,g,h,j) Usnea contexta 2.64 [+ or -] 0.61 1.34 [+ or -] 0.15 (b,c,g,h,i) FRAP ([micro]mol DPPH E[C.sub.50] of [Fe.sup.2+] Lichen species ([micro]g/mL) eq/g sample) Bulbothrix 851 (b,c,d,f ,g,i,j) 180 [+ or -] 3 setschwanensis (b,c,e,f,g,i,j) Flavoparmelia 3216 (a,c,d,e,g,h,i,j) 122 [+ or -] 2 caperata (a,c,d,e,f,g,h,i,j) Flavoparmelia 582 (a,b,d,e,f,i,j) 273 [+ or -] 2 euplecta (a,b,d,e,h,i,j) Flavoparmelia 1444 (a,b,c,e,f,g,h,i) 165 [+ or -] 4 haysomii (b,c,e,f,g,i,j) Hypotrachyna 946 (b,c,d,f,g,i) 316 [+ or -] 3 cirrhata (a,b,c,d,f,g,h,i,j) Lethariella 2894 (a,c,d,e,g,h,i,j) 290 [+ or -] 3 canariensis (a,b,d,e,h,i,j) Myelochroa 384 (a,b,d,e,f,i,j) 266 [+ or -] 6 irrugans (a,b,d,e,h,i,j) Parmelia 680 (b,d,f,i,j) 174 [+ or -] 2 omphalodes (b,c,e,f,g,i,j) Usnea 2446 (a,b,c,d,f,g,h,j) 98 [+ or -] 2 aurantiacoatra (a,c,d,e,f,g,h) Usnea contexta 1332 (a,b,c,f,g,i) 100 [+ or -] 1 (b,c,d,e,f,g,h) Phenolic content Lichen species ([micro]g GA/mg) Bulbothrix 52.2 [+ or -] 0.2 setschwanensis Flavoparmelia 49.2 [+ or -] 0.2 caperata Flavoparmelia 101.4 [+ or -]0.3 euplecta Flavoparmelia 35.2 [+ or -] 0.3 haysomii Hypotrachyna 60.6 [+ or -] 0.2 cirrhata Lethariella 50.6 [+ or -] 0.2 canariensis Myelochroa 92.5 [+ or -] 0.3 irrugans Parmelia 65.0 [+ or -] 0.2 omphalodes Usnea 22.4 [+ or -] 0.3 aurantiacoatra Usnea contexta 20.7 [+ or -] 0.2 Table 2: Values of L[D.sub.50] for the methanol extracts of the ten studied Parmeliaceae lichens species towards MCF-7 and HepG2 cells in treatments of 24 h. L[D.sub.50] L[D.sub.50] Lichen species ([micro]g/ml) ([micro]g/ml) MCF-7 HepG2 Bulbothrix setschwanensis 91 92 Flavoparmelia caperata 131 272 Flavoparmelia euplecta 67 67 Flavoparmelia haysomii 212 193 Hypotrachyna cirrhata 281 41 Lethariella canariensis 66 351 Myelochroa irrugans 145 22 Parmelia omphalodes 138 260 Usnea aurantiacoatra 339 151 Usnea contexta 208 54
|Printer friendly Cite/link Email Feedback|
|Title Annotation:||Research Article|
|Author:||Fernandez-Moriano, C.; Gonzalez-Burgos, E.; Divakar, P.K.; Crespo, A.; Gomez- Serranillos, M.P.|
|Publication:||Evidence - Based Complementary and Alternative Medicine|
|Date:||Jan 1, 2017|
|Previous Article:||Clinical Effect of Electroacupuncture on Lung Injury Patients Caused by Severe Acute Pancreatitis.|
|Next Article:||The Hypocholesterolemic Effects of Eryngium carlinae F. Delaroche Are Mediated by the Involvement of the Intestinal Transporters ABCG5 and ABCG8.|