Printer Friendly

Evaluation of quantitative parameters of Leydig cell in diabetic adults rats/Avaliacao de parametros quantitativos de celulas de Leydig em ratos diabeticos adultos.


Diabetes is a metabolic disorder characterized by blood glucose levels above 126 mg [dL.sup.-1], after fasting for at least eight hours, while normal levels are those below 100 mg [dL.sup.-1]. Actually, the disease is classified in diabetes mellitus type 1, type 2, gestational and others specific types (SEINO et al., 2010). Type 1 diabetes occurs by deficiency in insulin production by damage in pancreatic beta cells and in type 2 diabetes, generally associated with obesity, the insulin production is normal, but there is an insulin resistance on tissues (VAN BELLE et al., 2011).

Male sexual dysfunctions have been studied in diabetic individuals; however, the mechanisms are poorly understood (SHRILATHA; URALIDHARA, 2007; RICCI et al., 2009). Experimental studies in rats reported reductions in the testicular weight (BAL et al., 2011; KIANIFARD et al., 2011; NAVARROCASADO et al., 2010), seminiferous epithelium height (KIANIFARD et al., 2011; MALLICK et al., 2007; NAVARRO-CASADO et al., 2010; TRINDADE et al., 2013), impaired seminiferous cell cycle (RICCI et al., 2009) and depletion of germ cells (KAPANOGLU et al., 2013; KHAKI et al., 2010; WANKEU-NYA et al., 2013).

Diabetes may affect the endocrine control of the spermatogenic process (AGBAJE et al., 2007). According to Ballester et al. (2004), lack of insulin promotes alterations in the follicle stimulating hormone levels (FSH) and indirectly reduction in the luteinizing hormone levels (LH) and androgen production.

Changes in testosterone levels in diabetic men may be related to alterations in the Leydig cell function (ARIKAWE et al., 2006; BALLESTER et al., 2004; RICCI et al., 2009; USLU et al., 2009). Ballester et al. (2005) observed that Leydig cell number, testosterone level and reproductive performance were restored in diabetic rats treated with tungstate.

The relationship between quantitative changes in Leydig cells and diabetes are not clear. Some studies have demonstrated a reduced number of these cells (ARIKAWE et al., 2006; BALLESTER et al., 2004; BALLESTER et al., 2005) while others showed a significant increase in the Leydig number (EL-ROUBY et al., 2013; HASSEN et al., 2007; SANGUINETTI et al., 1995). Therefore, the goal of the present study was to evaluate the effects of diabetes on quantitative parameters of Leydig cells.

Material and methods

Experimental design

Twelve adult male Wistar rats (Rattus norvegicus, var. albinus), 70 days of age (200-300 g), were assigned to the following groups: Diabetic Group (DG; n = 6) and Control Group (CG; n = 6). Each group had free access to pelleted food and water until the end of the experiment. They were kept in a 12 hours reverse light [dark.sup.-1] cycle, with controlled humidity (50%) and temperature (22[degrees]C) in the vivarium of the Anatomy Department of Federal University of Pernambuco (UFPE). This study was approved by the ethics committee on animal experimentation of the UFPE (58/08 from 9/17/2008).

After fasting for 14 hours, diabetes was induced by a single intraperitoneal injection of streptozotocin (60 mg [kg.sup.-1]) according to Silva et al. (2011). The Control Group received citrate buffer (vehicle) by the same way. Animals were considered diabetic when presented glucose levels above 200 mg [dL.sup.-1]. The glycemic index was checked weekly and before euthanasia by blood collection from the caudal vein (Glucometer Kit- Accu Chek Active).

After eight weeks, rats were weighed, anesthetized with xylazine (5 mg [kg.sup.-1]) and ketamine (80 mg [kg.sup.-1]) and subjected to euthanasia (FANTONI; CORTOPASSI, 1996). All testicles were collected, weighed and fixed by immersion in 4% paraformaldehyde in phosphate buffer solution. Samples were routinely embedded in paraffin and fragments with 5 [micro]m thickness were stained with hematoxylin-eosin. Quantitative testicular analysis was performed according to Valenca et al. (2013) and the gonadosomatic index (GSI = [testicular weight/body weight] x 100]) was calculated as proposed by Caldeira et al.(2010).

Morphometric analysis

Volumetric density (%)

Volume densities of the testicular tissue components were obtained by 441-intersection grid placed in the ocular of a light microscope. Ten fields, randomly chosen (6615 points), were scored for each animal at 400X magnification. The intersection points over testicular parenchyma tissue were considered as following: tubular compartment (tunica propria, seminiferous epithelium and lumen) and intertubular space (Leydig cells, connective tissue, blood and lymphatic vessels). According to Valenca et al. (2013), the volume of each testicular component (mL) was established by the product of percentage points of testicular component (volumetric density %) and testis volume (testis net weight) (Figure 1).

Nuclear diameter of leydig cells

Thirty nuclear profiles were captured at 1000x magnification and analyzed with the software LAZ EZ 1.6.0. The nuclear diameter was obtained by means of two diametrically opposite measurements, according to Saraiva et al. (2006).

Volumetric density of leydig

The Leydig cells volumetric density (%) was measured using a reticule with 441 points (coupled in microscope) at 1000x magnification. A thousand points were counted, considering nucleus and cell cytoplasm. After this, the individual volume of the Leydig cell was determined with mathematical models, using the values of nuclear diameter and proportion in the testis.

Nuclear volume = 4/3 [pi][R.sup.3] R = nuclear radius;

Cytoplasmic Volume = % cytoplasm x nuclear volume/ % nucleus;

Cell Volume = nuclear volume + cytoplasmic volume;

The Leydig cell population was obtained using the values of individual volume of Leydig cell, volumetric density (%) and total volume (mL) occupied by Leydig cells in the testes.

Statistical analysis

Data were analyzed using t-student or Mann-Whitney tests in the program Sigmastat 3.5 after testing for normality. Data were expressed as mean ([+ or -]) standard deviation and median (minimum and maximum values). All statistical analysis was outlined for p < 0.05.


Glycemic index, body weight, testicular weight and gonadosomatic index

The diabetes induced by streptozotocin was efficient since the average glycemic level in the DG [526.5 (439-579) mg [dL.sup.-1]] increased 84% (p = 0.002) when compared with CG [85.5 (83-90) mg [dL.sup.-1]].

According to Figure 2, adult male rats with high glucose levels presented reduction in body (263 g [+ or -] 20.8; p [less than or equal to] 0.001) and testicular weight [1.422 (0.992-1.660) g; p = 0. 026] when compared with the control group [BW = 427 g [+ or -] 22.01; TW = 1.708 (1.693-1.786) g]. There was no significant difference in the gonadosomatic index between experimental groups, even though this index was higher in diabetic rats (1.05 % [+ or -] 0.146) than in non-diabetic rats (0.81% [+ or -] 0.313).

The volumetric density of the seminiferous tubule in DG (92.06% [+ or -] 0.699) did not differ from CG (93.06% [+ or -] 0.298). However, intertubular space increased in the diabetic group (8.34% [+ or -] 0.499; p= 0.007) when compared with non-diabetic animals (6.46% [+ or -] 0.256) (Figure 3).

Nuclear diameter, individual volume and population of leydig cells

According to Figure 2, Leydig cells nuclear diameter ([micro]m), nuclear volume ([micro][m.sup.3]) and cell volume were decreased in DG (ND = 4.08 [micro]m [+ or -] 0.08; p = 0.033; NV = 35.88 [micro][m.sup.3] [+ or -] 2.18; p = 0.041; CV = 394.7 [micro][m.sup.3] [+ or -] 24.03; p = 0.041) when compared to CG (ND = 4.88 [micro]m [+ or -] 0.312; NV = 64.74 [micro][m.sup.3] [+ or -] 12.1; CV = 712.1 [micro][m.sup.3] [+ or -] 133.1). The population of Leydig cell per gram of testis was 82 % higher in DG (418.6 [+ or -] 41.2 x [10.sup.6]; p = 0.01) than CG (230.3 [+ or -] 44.1 x [10.sup.6]).

The interstitial compartment is illustrated in Figure 4, which presents the arrangement of Leydig cells in animals of the control and diabetic groups.


Diabetes is a metabolic disorder associated with some characteristic symptoms as glycosuria, weight loss and polydipsia. Over time, high glucose levels promotes various complications including retinopathy, neuropathy, nephropathy, cardiovascular symptoms and sexual dysfunction (SEINO et al., 2010; VAN BELLE et al., 2011).

In the present study, the body weight reduction observed in diabetic animals corroborated Ricci et al. (2009). This reduction can be related to excessive urinary water and electrolytes loss (CHIASSON et al., 2003). Furthermore, low levels of insulin increases fat mobilization by activation of lipases, promoting triglycerides breakdown and release of free fatty acids (CHIASSON et al., 2003).

Testicular weight is a parameter related to seminiferous tubules length, Sertoli cells population and sperm production (FRANCA; RUSSELL, 1998). The testicular weight reduction (22.6%) observed in the current study corroborate previous studies in diabetic animals (BALLESTER et al., 2004; KHAKI et al., 2010; KIANIFARD et al., 2012; YULUG et al., 2013).

The gonadosomatic index (GSI = [testicular weight/ body weight] x 100) indicates the percentage of body weight allocated to the testis (CALDEIRA et al., 2010). In the current experiment, the GSI did not differ between groups, but in diabetic animals, the reduction in body and testicular weight was proportional, justifying the absence of difference. However, the GSI of the diabetic rats tended to increase, as previously described by Kianifard et al. (2011).

In this study, no change was observed in the tubular compartment between groups. Nevertheless, Ricci et al. (2009) observed reduced size and atrophy of seminiferous tubules in diabetic animals. These changes are probably related to reduction in FSH levels since low insulin levels affect directly testosterone and FSH levels (BALLESTER et al., 2004; KIANIFARD et al., 2012).

In the current study, the interstitial compartment was increased (29 %) in diabetic rats. This improvement may be related with increased thickness in the walls of blood vessels and higher deposition of extracellular matrix, observed in diabetics (BALLESTER et al., 2004; HASSEN et al., 2007).

Population of Leydig cells per gram of testis and individual volume vary among species. In mammalians, the volume of each Leydig cell ranges from 1000 [micro][m.sup.3] to 2000 [micro][m.sup.3] as well as the number per testis (FRANCA; RUSSEL, 1998).

Smooth endoplasmic reticulum (SER) is the most extensive organelle of Leydig cells (SHIRAI et al., 2013). The individual volume reduction of Leydig cells observed in the current study could be related to reduction of SER in the Leydig cells of the diabetic rats (KIANIFARD et al., 2012). For instance, Leydig cells function can be impaired since endoplasmic reticulum is directly related to androgen production (HASSEN et al., 2007). Furthermore, the low levels of LH and testosterone observed in diabetics by Ballester et al. (2004) can be related to the reduction of Leydig cells volume in this study. Therefore, these hormonal alterations influence the mechanisms related to proliferation, differentiation and function of Leydig cells, promoting changes in the number of these cells (KHAKI et al., 2010; NASROLAHI et al., 2013; SCHOELLER et al., 2012).

The Leydig cells number was increased in diabetic rats in the present study and corroborates Sanguinetti et al. (1995), Hassen et al. (2007) and El-Rouby et al. (2013). However, other studies pointed out a reduction in the number of these cells (ARIKAWE et al., 2006; BALLESTER et al., 2004; BALLESTER et al., 2005). On the other hand, Cameron et al. (1985) found no structural differences, in the number or function of Leydig cells. El-Rouby et al. (2013) mentioned that this variation between studies might be related to various factors as induction protocol, duration of the experiment and differences in species.


In conclusion, streptozotocin-induced diabetic rats show changes in the volumetric density between testicular compartments. In addition, absence of normal insulin levels affects the individual volume and population of Leydig cells.

Doi: 10.4025/actascibiolsci.v36i4.22455


AGBAJE, I. M.; ROGERS, D. A.; MCVILAR, C. M.; MCLURE, N.; ATHINKSON, A. B.; MALLIDIS, C.; LEWIS, S. E. Insulin dependent diabetes mellitus: implications for male reproductive function. Human Reproduction, v. 22, n. 7, p. 1871-1877, 2007.

ARIKAWE, A. P.; DARAMOLA, A. O.; ODOFIN, A. O.; OBIKA, L. F. Alloxan-induced and insulin-resistant diabetes mellitus affect semen parameters and impair spermatogenesis in male rats. African Journal of Reproductive Health, v. 10, n. 3, p. 106-113, 2006.

BAL, R.; TURK, G.; TUZCU, M.; YILMAZ, O.; OZERCAN, I.; KULOGLU, T.; GUR, S.; NEDZVETSKY, V. S.; TYKHOMYROV, A. A.; ANDRIEVSKY, G. V.; BAYDAS, G.; NAZIROGLU, M. Protective effects of nanostructures of hydrate C(60) fullerene on reproductive function in streptozotocin-diabetic male rats. Toxicology, v. 282, n. 3, p. 69-81, 2011.

BALLESTER, J.; DOMINGUEZ, J.; MUNOZ, M. C.; SENSAT, M.; RIGAU, T.; GUINOVART, J. J.; RODRIGUEZ-GIL, J. E. Tungstate treatment improves leydig cell function in streptozotocin-diabetic rats. Journal of Andrology, v. 26, n. 6, p. 706-715, 2005.

BALLESTER, J.; MUNOZ, M. C.; DOMINGUEZ, J.; RIGAU, T.; GUINOVART, J. J.; RODRIGUEZ-GIL, J. E. Insulin-dependent diabetes affects testicular function by FSH and LH-linked mechanisms. Journal of Andrology, v. 25, n. 5, p. 706-719, 2004.

CALDEIRA, B. C.; PAULA, T. A. R.; MATTA, S. L. P.; BALARINI, M. K.; CAMPOS, P. K. A. Morphometry of testis and seminiferous tubules of the adult crab-eating fox (Cerdocyonthous, Linnaeus, 1766). Revista Ceres, v. 57, n. 5, p. 569-575, 2010.

CAMERON, D. F.; MURRAY, F. T.; DRYLIE D. D. Interstitial compartment pathology and spermatogenic disruption in testes from impotent diabetic men. The Anatomical Record, v. 213, n. 1, p. 53-62, 1985.

CHIASSON, J. L.; ARIS-JILWAN, N.; BELANGER, R.; BERTRAND, S.; BEAUREGARD, H.; EKOE, J. M.; FOURNIER, H.; HAVRANKOVA, J. Diagnosis and treatment of diabetic ketoacidosis and the hyperglycemic hyperosmolar state. Canadian Medical Association Journal, v. 168, n. 7, p. 859-866, 2003.

EL-ROUBY, N. M. M. A.; BAYUMI, F. A.; GAWISH, S. M. Early administration of insulin glargine improved expression of endothelial nitric oxide synthase in the testes of diabetic rats. Acta Biologica Malaysiana, v. 2, n. 1, p. 1-11, 2013.

FANTONI, D. T.; CORTOPASSI, S. R. G. Tecnicas anestesicas em animais de laboratorio. In: DE LUCA, R. R. (Org.) Manual para tecnicos de bioterio. 2. ed. Sao Paulo: Winner Graph, 1996. p. 157-164.

FRANCA, L. R.; RUSSELL, L. D. The testis of domestic animals. In: MARTINEZ-GARCIA, F.; REGADERA, J. (Ed.). Male reproduction: a multidisciplinary overview, 1st ed. Madrid: Churchill Levingstone, 1998. v. 1, p. 197-219.

HASSEN, N. S.; ROUBI, N. M. E. I.; OMARA, E. A. Evaluation of the influence of each of melatonin and chromium against diabetes-induced alteration in the testis of albino rats using light and electron microscopies. Egyptian Journal of Hospital Medicine, v. 27, n. 2 p. 143-162, 2007.

KAPANOGLU, G. T.; BAHCELIOGLU, M.; GOZIL, R.; MELVACIOGLU, F.; BURU, E.; TEKINDAL, M. A.; ERDOGAN, D.; CALGUNER, E. Effects of green tea and vitamin E in the testicular tissue of streptozotocin-induced diabetic rats. Saudi Medical Journal, v. 34, n. 7, p. 734-743, 2013.

KHAKI, A.; FATHIAZAD, F.; NOURI, M.; KHAKI, A.; MALEKI, N. A.; KHAMNEI, H. J.; AHMADI, P. Beneficial effects of quercetin on sperm parameters in streptozotocin-induced diabetic male rats. Phytotherapy Research, v. 24, n. 9, p. 1285-1291, 2010.

KIANIFARD, D.; SADRKHANLOU, R. A.; HASANZADEH, S. The histological, histomorphometrical and histochemical changes of testicular tissue in the metmorfin treated and untreated streptozotocin-induced adult diabetic rats. Veterinary Research Forum, v. 2, n. 1, p. 13-24, 2011.

KIANIFARD, D.; SADRKHANLOU, R. A.; HASANZADEH, S. The ultrastrutural changes of the sertoli and leydig cells following streptozotocin-induced diabetes. Iran Journal of Basic Medical Science, v. 15, n. 1, p. 623-35, 2012.

MALLICK, C.; MANDAL, S.; BARIK, B.; BHATTACHARYA, A.; GHOSH, D. Protection of testicular dysfunctions by MTEC, a formulated herbal drug, in streptozotocin induced diabetic rat. Biological and Pharmaceutical Bulletin, v. 30, n. 1, p. 84-90, 2007.

NASROLAHI, O.; KHANESHI, F.; RAHMANI, F.; RAZI, M. Honey and metmorfin ameliorated diabetes-induced damages in testes of rat: correlation with hormonal changes. Iranian Journal of Reproductive Medicine, v.11, n.12, p. 1013-1020, 2013.

NAVARRO-CASADO, L.; JUNCOS-TOBAMA, M. A.; CHALER-RUDELLA, M.; ONZONO, L. I.; BLAZQUEZ-CABRERA, J. A.; MIRALLES-GARCIA, J. M. Effect of experimental diabetes and STZ on male fertility capacity: study in rats. Journal of Andrology, v. 31, n. 6, p. 584-592, 2010.

RICCI, G.; CATIZONE, A.; ESPOSITO, R.; PISANTI, F. A.; VIETRI, M. T.; GALDARI, M. Diabetic rat testes: morphological and functional alterations. Andrologia, v. 41, n. 6, p. 361-368, 2009.

SANGUINETTI, R. E.; OGAWA, K.; KUROHMARU, M.; HAYASHI, I. Ultrastructural changes in mouse leydig cells after streptozotocin administration. Experimental Animals, v. 44, n. 1, p. 71-73, 1995.

SARAIVA, K. L. A.; SILVA JUNIOR, V. A.; DIAS, E. S. F.; PEIXOTO, C. A. Morphological changes in the testis induced by diethylcarbamazine. Reproductive Toxicology, v. 22, n. 4, p. 754-759, 2006.

SCHOELLER, E. L.; ALBANNA, G.; FROLOVA, A. I.; MOLEY, K. M. Insulin rescues impaired spermatogenesis via the hypothalamic-pituitary-gonadal axis in Akita diabetic mice and restores male fertility. Diabetes, v. 61, n. 7, p. 1869-1878, 2012.

SHRILATHA, B.; MURALIDHARA, D. R. Occurrence of oxidative impairments, response of antioxidant defences and associated biochemical perturbations in male reproductive milieu in the streptozotocin-diabetic rat. International Journal of Andrology, v. 30, n. 6, p. 508-518, 2007.

SHIRAI, M.; WAKUI, S.; WEMPE, M. F.; MUTOU, T.; OYAMA, N.; MOTOHASHI, M.; TAKAHASI, H.; KANSAKU, N.; ASARI, M.; HANO, H.; ENDOU, H. Male Sprague-dawley rats exposed to in utero di(n-butyl) phthalate dose dependent and age-related morphological changes in Leydig cell smooth endoplasmic reticulum. Toxicologic Pathology, v. 41, n. 7, p. 984-991, 2013.

SEINO, Y.; NANJO, K.; TAJIMA, N.; KADOWAKI, T.; KASHIWAGI, A.; ARAKI, E.; ITO, C.; INAGAKI, N.; IWAMOTO, Y.; KASUGA, M.; HANAFUSA, T.; HANEDA, M.; UEKI, K. Report of the committee on the classification and diagnostic criteria of Diabetes mellitus. Journal of Diabetes Investigation, v. 1, n. 5, p. 212-228, 2010.

SILVA, M. F.; PELUZIO, M. C. G.; AMORIM, P. R. S.; LAVORATO, V. N.; SANTOS, N. P.; BOZI, L. H. M.; PENITENTE, A. R.; FALKOSKI, D. L.; BERFORT, F. G.; NATALI, A. J. Swimming training attenuates contractile dysfunction in diabetic rats cardiomyocytes. Arquivos Brasileiros de Cardiologia, v. 97, n. 1, p. 33-39, 2011.

TRINDADE, A. A. T.; SIMOES, A. C. P.; SILVA, R. J.; MACEDO, C. S.; SPADELLA, C. T. Long term evaluation of morphometric and ultrastructural changes of testes of alloxan-induced diabetic rats. Acta Cirurgica Brasileira, v. 28, n. 4, p. 256-265, 2013.

USLU, B. A.; LLHAN, F.; GULYUZF, F.; KARACA, M.; ONER, A. C. Assessment of the histopathological changes occurring in the testis of the mice suffering from the experimental diabetes induced using alloxan. Journal of Animal and Veterinary Advances, v. 8, n. 10, p. 1929-1935, 2009.

VALENCA, R. M. B.; SILVA JUNIOR, V. A.; ARAUJO, L. P. C.; REIS, J. C.; GUERRA, M. M. P.; SOARES, P. C.; COSTA, A. N. Morphometry and histomorphometry of testis in crossbred pigs fed diets with different proteins levels. Arquivo Brasileiro de Medicina Veterinaria e Zootecnia, v. 65, n. 5, p. 1329-1338, 2013.

VAN BELLE, T.; COPPIETERS, K.; VON HERRATH, M. Type 1 diabetes: etiology, immunology, and therapeutic strategies. Physiological Reviews, v. 91, n. 1, p. 79-118, 2011.

WANKEU-NYA, M.; FLOREA, A.; BALICI, S.; WATCHO, P.; MATEI, H.; KAMANYI, A. Dracaena arborea alleviates ultrastructural spermatogenic alterations in streptozotocin-induced diabetic rats. BMC Complementary and Alternative Medicine, v. 13, n. 71, p. 1-9, 2013.

YULUG, E.; TURED, S.; ALVER, A.; KUTLU, O.; KARAGUZEL, E.; KAHRAMAN, C. Effects of resveratrol on testis damage in streptozotocin induced diabetic rats. Journal of Animal and Veterinary Advances, v. 12, n. 6, p. 747-753, 2013.

Received on November 21, 2013.

Accepted on May 14, 2014.

Alluanan Adelson do Nascimento Silva (1) *, Jessica Santana de Oliveira (1), Rodrigo Ribeiro de Oliveira (2), Silvia Regina Arruda de Moraes (3), Valdemiro Amaro da Silva Junior (1) and Elizabeth Neves de Melo (3)

(1) Departamento de Morfologia e Fisiologia Animal, Universidade Federal Rural de Pernambuco, Rua Dom Manoel de Medeiros, s/n, Dois Irmaos, 52171-900, Recife, Pernambuco, Brazil. (2) Departamento de Fisioterapia, Universidade Federal do Ceara, Fortaleza, Ceara, Brazil. (3) Departamento de Anatomia, Universidade Federal de Pernambuco, Recife, Pernambuco, Brazil. * Author for correspondence. E-mail:
COPYRIGHT 2014 Universidade Estadual de Maringa
No portion of this article can be reproduced without the express written permission from the copyright holder.
Copyright 2014 Gale, Cengage Learning. All rights reserved.

Article Details
Printer friendly Cite/link Email Feedback
Title Annotation:texto en ingles
Author:Silva, Alluanan Adelson do Nascimento; de Oliveira, Jessica Santana; de Oliveira, Rodrigo Ribeiro; d
Publication:Acta Scientiarum. Biological Sciences (UEM)
Date:Oct 1, 2014
Previous Article:Assessment of DNA extraction methods for detection of arbuscular mycorrhizal fungi in plant roots by nested-PCR/Avaliacao de metodos de extracao de...
Next Article:Body and testicular biometric parameters of the scorpion mud turtle (Kinosternon scorpioides)/Parametros biometricos corporais e testiculares de...

Terms of use | Privacy policy | Copyright © 2019 Farlex, Inc. | Feedback | For webmasters