Evaluation of litchi chinensis for host status to Xyleborus glabratus (Coleoptera: Curculionidae: Scolytinae) and susceptibility to laurel wilt disease.
To date, 12 U.S. species--all in the angiosperm family Lauraceae--have been reported as hosts of X. glabratus and/or have shown susceptibility to laurel wilt in nature or through laboratory inoculations with R. lauricola (Fraedrich et al. 2008; Hanula et al. 2008; Mayfield et al. 2008, 2013; Mayfield & Hanula 2012; Ploetz & Konkol 2013). Chemical ecology studies provide evidence that dispersing beetles locate appropriate hosts based on their volatile emissions; the current hypothesis is that females respond to a blend of terpenoid compounds (a generalized 'laurel bouquet'), with [alpha]-copaene as the primary long-range attractant (Hanula & Sullivan 2008; Niogret et al. 2011a; Kendra et al. 2011, 2012a, 2012c; Hanula et al. 2013). In early studies, freshly-cut logs (bolts) of lychee (Litchi chinensis Sonn., Sapindales: Sapindaceae) were evaluated as a positive control for attraction of X. glabratus (Kendra et al. 2011). Lychee wood was chosen based on its high content of [alpha]-copaene (Niogret et al. 2011b), but it was presumed to be a non-host since the species is not a member of the Lauraceae. In the field, lychee bolts (from an unknown cultivar) were found to be highly attractive to X. glabratus, capturing significantly more beetles than host avocado wood or commercial manuka oil lures (Kendra et al. 2011). In no-choice laboratory bioassays conducted with the same lychee germplasm, more than half of X. glabratus females initiated boring when presented with lychee bolts.
Based on the latter observation, a study was initiated to evaluate host status of two commercial lychee cultivars, 'Brewster' and 'Mauritius', varieties planted extensively in south Florida (Crane et al. 2008). Since lychee is a subtropical fruit tree grown in close proximity to avocado groves in Miami-Dade County, lychee could potentially function as a reservoir for R. lauricola and/or X. glabratus even if it is not susceptible to laurel wilt. Several laboratory, greenhouse, and field experiments were conducted with 'Brewster' and 'Mauritius' to determine if these cultivars were susceptible to attack by X. glabratus and, if so, whether they develop symptoms of laurel wilt and support reproduction of X. glabratus. Plants were also artificially inoculated with R. lauricola to assess colonization of this host by the fungus and whether laurel wilt could be induced in this manner. In addition, we performed chemical analysis of the volatile emissions from lychee to determine how its sesquiterpene composition compares to that from known lauraceous hosts.
MATERIALS AND METHODS
Our initial experiment was a series of no-choice laboratory bioassays to assess host recognition and boring behaviors, following methods previously published (Kendra et al. 2011). Test substrates consisted of freshly-cut bolts (5 cm diam. x 15 cm length) from four trees: (1) L. chinensis cv. 'Brewster' [obtained from the USDA-ARS Germplasm Repository, Miami, Florida (WA3-2461, MIA# 17454, PI# 277462)], (2) L. chinensis cv. 'Mauritius' [USDA-ARS (WA3-20-64, MIA# 17441, PI# 277473)], (3) silkbay Persea humilis Nash [a known host and positive control (Fraedrich et al. 2008), found to be the most attractive native Persea in previous field tests (Kendra et al. 2013)], and (4) live oak Quercus virginiana Mill. [Fagaceae, a non-host and negative control (Fraedrich et al. 2008; Mayfield et al. 2008; Kendra et al. 2011)]. Bolts of silkbay and live oak were collected from sites along the Lake Wales Ridge in central Florida (Highlands County). Test insects were host-seeking female X. glabratus collected at those same field sites using a baiting method described previously (Kendra et al. 2012b). Insects were captured during the late afternoon/ early evening, held overnight in plastic boxes containing moist tissue paper, and used in bioassays early the next morning. Assays were conducted at Archbold Biological Station (Lake Placid, Florida) under controlled laboratory conditions (25[degrees]C, 16:8 h L:D). Test arenas consisted of 4.4-L plastic buckets covered with cheese cloth mesh, secured with rubber bands, and held in screened insect cages (BioQuip, Rancho Dominguez, California) during the experiment (Fig. 1A). In each bucket, lined with a filter paper disk (15 cm diam; Whatman Intl. Ltd., Maidstone, England), were placed 10-15 beetles and a single bolt of wood (Fig. 1B). A beetle was scored positive for boring when it was perpendicular to the wood substrate and half its body length (~1 mm) was inserted into the entrance hole (Fig. 1C). [Previous observations indicated that females sometimes 'sampled' the substrate (making shallow bore holes) but then aborted the attempt; however, once they inserted half their body length, they continued to bore through the bark and cambium layers into the sapwood.] The number of beetles that were boring and their location on the bolt were recorded at 1, 2, 4, 8, 12, 24, and 48 h. Tests were replicated 5 times for each substrate, and each replicate was run using a separate arena, a new bolt of wood, and a new cohort of beetles.
Field Infestation Experiment
A second no-choice experiment was conducted to determine if X. glabratus would bore into live lychee trees and transmit R. lauricola, using protocols similar to those of Mayfield et al. (2008) for evaluation of avocado host status. Test substrates consisted of four potted 'Brewster' trees of two size classes, maintained outdoors under irrigation at the Lake Wales Ridge Environmental Management Area, Royce Ranch Unit, Highlands County, Florida (N 27[degrees] 22' 464", W 81[degrees] 20' 212"). Two small trees (Trees A and B), each 2.5 cm trunk diam at soil level, were purchased from a commercial nursery in Homestead, Florida. Two larger trees (Trees C and D), with trunk diam of 11. 4 and 13.8 cm, were obtained from a local lychee grower by air-layering of branches from mature trees in his grove. With each of the four trees, one branch was sawed off (flush with the trunk) to provide a freshly-cut surface, since previous studies had shown a preference by X. glabratus to initiate boring at cut or wounded sites (Kendra et al. 2011). The portion of the trunk containing the cut surface was enclosed in a mesh insect sleeve (20 x 40 cm, BioQuip) which contained a zippered access port and a clear plastic window to allow for observations (Fig. 2A). Two thin metal hoops were placed inside the sleeve and secured with binder clips, which prevented the sleeve from collapsing and further facilitated observations. Each sleeved trunk then received 10 female X. glabratus ([less than or equal to] 3 d post-emergence), obtained from the University of Florida laboratory colony, which had been reared under conditions described by Brar et al. (2013). The experiment was initiated in the early evening (18:00-18:30 h EDST), since this time period had been identified as the peak window during which female X. glabratus engage in host-seeking flight in Florida (Brar et al. 2012; Kendra et al. 2012a). Initial observations (to confirm boring) were made after 1 h and 16 h (the next morning), and then the condition of the trees was checked thereafter at approximately 2-3 wk intervals for 3 mo. At the conclusion of the experiment, wood was dissected near the beetle entrance holes to examine gallery formation and search for evidence of X. glabratus reproduction.
In addition, tissue associated with five (Tree C) and six (Tree D) galleries of X. glabratus was assayed for R. lauricola. Brownish discoloration, often quite conspicuous, was observed adjacent to galleries when trunks were dissected lengthwise with a bandsaw (Fig. 3). With a hammer and chisel, discolored wood was sampled ca 0.5 cm from the above galleries, surface-disinfested for 15 sec in 70% ethanol and then 2 min in 10% bleach, rinsed in sterile water, and blotted dry with sterile Kimwipes before placement on a semi-selective medium, CSMA+, in 10-cm-diam Petri plates (Ploetz et al. 2012). Plates were incubated on a laboratory bench for 10 d under ambient light before examination for growth characteristic of R. lauricola.
Artificial Inoculation of Lychee with Raffaelea lauricola
RL4, an isolate of R. lauricola that kills lauraceous hosts of this pathogen (Ploetz et al. 2012; Ploetz & Konkol 2013), is deposited as CBS 127349 at the Centraalbureau voor Schimmelcultures (CBS Fungal Biodiversity Centre, Utrecht, The Netherlands); it was removed from -80[degrees]C storage and grown on malt extract agar (MEA) for use in inoculation experiments. Trees of cvs. 'Brewster' and 'Mauritius' (air-layered plants in 12-L pots) were obtained from a local commercial nursery and maintained in an air-conditioned greenhouse (ca 28[degrees]C) at the University of Florida's Tropical Research & Education Center in Homestead, Florida. Three trees of each cv were each inoculated ~20 cm above the soil line with 105 conidia of RL4 as described previously (Ploetz et al. 2012).
After 1 mo, trees were observed externally and internally for disease symptoms, including chlorotic and necrotic foliage, vascular discoloration, discharge from the inoculation sites, and any other indication of a pathological interaction of RL4 with inoculated trees. In addition, sapwood was sampled as above and assayed for R. lauricola. The experiment was conducted twice.
Chemical Collection and Analysis
Samples for chemical analysis were prepared by manually rasping the outer layers of bark and cambial tissue from freshly cut bolts (5 cm diam) of lychee cv. 'Brewster', silkbay, and live oak (identical to those used in bioassays above), using methods reported by Niogret et al. (2011a). Volatile chemicals were collected from the rasped shavings (6-g samples, 3 replicates per tree) by using Super Q traps (Analytical Research Systems, Gainesville, Florida) according to published methods (Heath & Manukian 1992; Heath et al. 1993). Samples were spread in a cylindrical glass chamber (4.5 cm diam x 25 cm length), purified air was introduced into the chamber (1 L/min), and headspace volatiles were collected for 15 min. Super Q traps were cleaned by soxhlet extraction with methylene chloride for 24 h and dried in a fume hood prior to each use. Volatile chemicals were eluted from the Super Q adsorbent with 200 [micro]L of high purity methylene chloride (99.5% pure; ACROS, Morris Plains, New Jersey). An aliquot of [C.sub.16] standard (5 [micro]g) was added to each sample for quantitative analysis. All chemical sampling was performed within 2 h of collection of the branch material.
Chemical extracts were analyzed by using gas-chromatography (ThermoQuest Trace GC 2000, Austin, Texas). The column was fused silica, 25 m long, 0.25 mm i.d. DB-5MS phase (J&W Scientific, Agilent Technologies, Santa Clara, California), programmed from 50 to 130[degrees]C at 15.0[degrees]C/ min, then from 130 to 220[degrees]C at 10.0[degrees]C/min, and then held at 220[degrees]C for 4 min. The column used in the gas chromatograph interface to the mass spectrometer (Agilent Technologies 5975B) was 25 m long, 0.25 mm i.d., DB-5MS phase (J&W Scientific, Agilent Technologies), programmed at 40[degrees]C for 2 min, then from 40 to 130[degrees]C at 10.0[degrees]C/ min, then from 130 to 220[degrees]C at 20.0[degrees]C/min, and then held at 220[degrees]C for 4 min. Chemicals were identified by using the NIST mass spectral program (version 2.0 d) and the NIST/EPA/NIH mass spectral library (NIST11) when Reverse Matches and Matches were > 950 and > 900%e, respectively. For each sesquiterpene, the Kovats Retention Index (RI) was compared with the RI calculated from synthetic chemicals when commercially available [RI = 1358, 1391, 1442, 1477, 1532 for [alpha]-cubebene (Bedoukian Research Inc., Danbury, Connecticut), [alpha]-copaene (Fluka Analytical, Stenheim, Germany), [beta]-caryophyllene (Sigma Chemical Co., St. Louis, Missouri), [alpha]-humulene (Sigma Chemical Co.), and [delta]-cadinene (Fluka Chemie, Buchs, Switzerland), respectively] or with previously published data [calamenene (Singh et al. 2007; Ibrahim et al. 2010)].
Regression analysis was used to document temporal patterns in boring behaviors observed in the no-choice laboratory bioassays (Systat Software 2010). Final results for percentage of boring observed with the bolt treatments was analyzed by one-way analysis of variance (ANOVA), followed by least significant difference test (LSD) for mean separation, P < 0.05 (Systat Software 2010).
Composite results of the no-choice boring bioassays are presented in Fig. 4. Regression analysis with sigmoidal models best described the relationships between the time after presentation of lychee or silkbay bolts and the percentage of females boring [Silkbay: y = 98.21 / (1 + [e.sup.-[(x - 4.12)/1.26]]), [R.sup.2] = 0.989; lychee cv. 'Brewster': y = 42.84 / (1 + [e.sup.-[x - 664)/1.94]), [R.sup.2] = 0.991; and lychee cv. 'Mauritius': y = 34.56 / (1 + [e.sup.-[x - 7.30)/2.55]), [R.sup.2] = 0.987]. Boring was initiated quickly on bolts of silkbay, with the maximum percentage achieved within ~8 h. With both cultivars of lychee, females spent considerably more time walking over the wood substrate before choosing a site and committing to boring behaviors. This resulted in a large lag time (relative to silkbay) in boring response, with the maximum percentage reached at 12-15 h, and no further increase recorded at the 24 or 48 h readings. Few beetles responded to the bolts of live oak; the majority continued to wander throughout the arena during the course of the experiment, but several females were observed to settle into natural crevices or under the bark at the cut ends of the bolts. This behavior was interpreted to represent a thigmotactic response; it was observed to varying degrees with all bolt treatments and was not scored as positive for boring in our bioassays. At the conclusion of the test (48 h), there were differences in boring among the bolt treatments (F = 78.03, df = 3,16; P < 0.001). Percentage (mean [+ or -] SE) of females boring into silkbay (97.78 [+ or -] 2.22 %) was significantly higher than into 'Brewster' (44.29 [+ or -] 5.25 %) or 'Mauritius' (35.00 [+ or -] 7.17 %; not statistically different from 'Brewster'), and all three were higher than live oak (0%). Regarding site selection on the bolts, the percentage of boring initiated on the cut surface corresponded to 60.4% for silkbay, 85.7% for 'Mauritius', and 100% for 'Brewster'.
Field Infestation Experiment
One hour after introduction into the sleeved enclosures, the majority of female X. glabratus were walking over the freshly cut surfaces where branches had been removed from the four test trees (Fig. 2B), but no boring was observed at this time (following the same criteria for positive boring applied in the laboratory bioassays). When checked 16 h later (at 10:00 h the next morning), three beetles were actively boring into the cut surface on one of the large diam trees (Tree C, Fig. 2C), and four beetles were boring into the cut surface on the second large tree (Tree D). No boring was observed at any other site (i.e. through the bark) along the trunks of Trees C and D, and no boring was observed with either of the two small diam trees (Trees A and B). When checked again 2 wk later, Tree C had four distinct entry holes (representing 40% boring; Fig. 2D) and Tree D had five entry holes (50% boring), all located on the cut surface. Both trees also had fresh 'sawdust' present around the entrance holes which indicated that there was active boring by X. glabratus. In contrast, neither small tree had evidence of boring. At this 2-wk inspection, the sleeves were removed from all trees, and the dead 'founding' females (that had not bored) were removed from the enclosures. The sleeves were then reapplied to collect any F1 (first filial generation) adult X. glabratus that might emerge. Observations made over the next few months showed no indication of laurel wilt symptoms in either large diam tree into which X. glabratus had bored.
At 3 mo when the experiment was terminated, the sleeves were removed and no adult X. glabratus were found. Tree dissections revealed that the majority of beetle galleries were fairly shallow, consisting of an entrance tunnel with little or no branching into secondary tunnels, the sites of X. glabratus brood galleries (Brar et al. 2013). Although wood adjacent to galleries was discolored (Fig. 3), typical colonies of R. lauricola were not recovered on CSMA+ from tissue pieces associated with 11 galleries (0 of 102 assays).
Artificial Inoculation of Lychee with Raffaelea lauricola
After 1 mo, disease symptoms did not develop externally or internally in plants of either 'Brewster' or 'Mauritius' that were inoculated with R. lauricola; RL4 had no discernible impact on the health of inoculated trees during both experiments. Moreover, in no case was the fungus recovered from inoculated tissue.
Table 1 presents the sesquiterpene emissions quantified from the bolt treatments used in the no-choice boring bioassays. Silkbay (which is highly aromatic) had the highest quantity of volatile sesquiterpenes as well as the largest diversity of sesquiterpene components. The five principal components (in descending order) emitted from silkbay (Fig. 5A) consisted of [beta]-caryophyllene (peak 5), [alpha]-copaene (peak 2), [alpha]-cubebene (peak 1), an unidentified sesquiterpene (peak 4), and calamenene (peak 8). Lychee cv. 'Brewster' (Fig. 5B) had significantly fewer peaks detectable within the sesquiter pene range, but there were several key constituents found in common with silkbay, including [beta]-caryophyllene, [alpha]-copaene, and [alpha]-humulene (peak 6); however, all were detected at much lower levels. Live oak (Fig. 5C) also emitted [beta]-caryophyllene and [alpha]-copaene, but at levels lower than lychee.
In a previous study, R. lauricola was the most common symbiont recovered from X. glabratus (86% of assayed females with an average of 2,783 colony forming units per individual) (Carrillo et al. 2013). Although female X. glabratus, presumably carrying conidia of R. lauricola within mandibular mycangia (Fraedrich et al. 2008), successfully bored into lychee in the present no-choice tests, R. lauricola was neither recovered from areas adjacent to galleries of X. glabratus, nor were vascular symptoms (typical for laurel wilt) observed. Furthermore, RL4, an isolate of R. lauricola that kills lauraceous host plants (Ploetz et al. 2012, Ploetz & Konkol 2013), had no discernible impact on artificially inoculated lychee. The available data indicate that lychee cvs. 'Brewster' and 'Mauritius' are not hosts for R. lauricola and not susceptible to laurel wilt. Since lychee is native to Southeast Asia, it may have had a coevolved history with endemic X. glabratus, resulting in resistance to R. lauricola (a known symbiont of X. glabratus in Asia as well; Harrington et al. 2011). Further investigations would be necessary to determine if resistance is due to an induced defensive response by lychee trees or simply due to an unfavorable internal environment within lychee wood (e.g., moisture content, nutrient levels, secondary metabolites). Regardless, the inability of lychee wood to support growth of R. lauricola, the presumed primary nutritional symbiont of X. glabratus, also leads to the conclusion that L. chinensis is not likely to serve as a suitable reproductive host for X. glabratus. This is supported further by the lack of F1 adults 3 mo after gallery initiation into 'Brewster' lychee trees. In confirmed hosts, it has been shown that teneral adults are present within the galleries after 1 mo, and dispersing females are observed after 2 mo (Brar et al. 2013). However, the current study focused primarily on the threat of X. glabratus and R. lauricola to healthy, live trees of lychee (a specialty fruit crop of economic importance in south Florida); we cannot rule out the possibility that X. glabratus may potentially reproduce in stressed/ dying lychee trees (from factors other than laurel wilt) which may have compromised resistance to ambrosia fungi. In lauraceous hosts, it has been hypothesized that optimal reproduction of X. glabratus is dependent on pre-colonization by R. lauricola; founding females may not necessarily lay eggs, but their inoculation of host trees with R. lauricola results in stress/disease conditions that facilitate successful reproduction of subsequent generations of X. glabratus (Fraedrich et al. 2008, 2011).
Although not a likely host for X. glabratus, lychee can be regarded as an effective 'chemical mimic' of the host Lauraceae. Lychee wood is attractive to dispersing, host-seeking females in the field (Kendra et al. 2011, 2012b), and therefore emits the appropriate long-range olfactory cues. Previous research with essential oils and lauraceous hosts suggests that [alpha]-copaene is the primary host attractant (kairomone), but other volatile sesquiterpenes may contribute; potential candidates include [alpha]-cubebene, [alpha]-humulene, [beta]-caryophyllene, [delta]-cadinene, and calamenene (Hanula & Sullivan 2008; Kendra et al. 2011, 2012c, 2013; Niogret et al. 2011a, 2013a; Hanula et al. 2013). The chemical analysis of 'Brewster' in this study indicated that its sesquiterpene profile was much less complex than that of silkbay. 'Brewster's three main components--[beta]-caryophyllene, [alpha]-copaene, and [alpha]-humulene--were also high in silkbay. Emissions of these same three sesquiterpenes were positively correlated with captures of X. glabratus in field tests with avocado bolts (three cultivars, Kendra et al. 2011), providing further support that these highly volatile terpenoids likely function as attractive kairomones.
In addition to attraction of X. glabratus, lychee wood also mimics a host in that it elicits boring behaviors from a significant proportion of females that make contact. This has been documented here with 'Brewster' (44%) and 'Mauritius' (35%), and previously with an unnamed cultivar (57%, Kendra et al. 2011). Although the lychee substrates may have been perceived as suboptimal, as evidenced by the longer assessment period (relative to silkbay) prior to boring observed in bioassays, lychee possesses sufficient secondary cues necessary for host recognition and acceptance by a subset of female X. glabratus. This phenomenon also has been observed with red maple Acer rubrum L. (Aceraceae), another non-host species. In no-choice tests with young potted plants, 40% of red maples were positive for X. glabratus boring, but those plants remained asymptomatic for laurel wilt, no R. lauricola were recovered, and no beetle reproduction was observed (Fraedrich et al. 2008). Little is known about the short-range secondary cues that trigger a behavioral switch from host-seeking to host-boring, but they may include olfactory, gustatory, and contact chemosensory cues in combination with tactile and visual stimuli (i.e., the wood substrate must smell, taste, feel, and look right in order for females to commit to boring). With either bolts or live trees of lychee, X. glabratus showed a strong boring preference for freshly-cut surfaces, presumably due to a higher release (threshold dose) of chemical stimulants from damaged tissue. In the bolt assay, significantly higher boring was obtained with silkbay, which emits much higher levels of sesquiterpenes than lychee. Therefore, the same chemicals responsible for long-range attraction may be associated with initiation of boring behaviors.
In the field infestation experiment, there was also evidence of a female preference for larger diam hosts (boring percentages observed with potted 'Brewster' trees were 0, 40, and 50% with the 2.5, 11.4, and 13.8 cm diam trunks, respectively). Although anecdotal, the observation is consistent with data collected from infested forest ecosystems. In a survey of swampbay trees symptomatic for laurel wilt (N = 280), no X. glabratus entry holes (0.8 mm diam, Hanula et al. 2008) were observed in trunks/branches less than ~2 cm diam, and there was a progressive increase in density of entry holes with increasing diam (Kendra et al. 2013). The oldest (largest diam) trees are typically the first to be attacked by X. glabratus and succumb to laurel wilt; once those trees are depleted, the vector then attacks progressively smaller diam trees (Kendra et al. 2013). A host 'diameter preference' has also been observed in two rare Lauraceae, pondberry Lindera melissifolia (Walter) Blume and pondspice Litsea aestivalis (L.) Fern; although both species support growth of R. lauricola and are susceptible to laurel wilt when subjected to laboratory inoculations, the two shrubby plants with small diam stems are infrequently attacked by X. glabratus in the field, even when in close proximity to infested redbay trees (Fraedrich et al. 2011). This selective behavior for large woody hosts would be adaptive for X. glabratus, since larger diam trees could physically allow for larger gallery formations (and more extensive fungal gardens) within the sapwood, which would facilitate increased beetle reproduction. A positive correlation between gallery length and number of progeny has been demonstrated previously for other ambrosia beetles, including Xylosandrus mutilatus Blandford (Karimura & Hijii 1994) and Xyleborus pfeili (Mizuno & Kajimura 2002). Recent research indicates that host diam is a visual host-seeking cue used by female X. glabratus (Mayfield & Brownie 2013), but most likely effective when accompanied by appropriate chemical cues. Proximo-distal gradients (from trunk to large diam branches to small diam braches) in terpenoids, including [alpha]-copaene, have been documented within avocado trees (Niogret et al. 2013a, 2013b); these chemical gradients, in combination with visual cues, may function in the discrimination process used by X. glabratus for site-selection on host trees.
In summary, our results indicate that lychee may be vulnerable to attack by X. glabratus, particularly at sites with recent damage (e.g. freshly pruned limbs), due to chemical similarities with the Lauraceae. However, lychee cvs. 'Brewster' and 'Mauritius' are not susceptible to laurel wilt disease and do not promote growth of R. lauricola. Consequently, these commercial cultivars are not likely to function as reproductive hosts for X. glabratus. The discovery that lychee chemically mimics the host Lauraceae, but has a much simpler volatile profile, may provide a serendipitous opportunity to better understand the chemical ecology of X. glabratus. Further evaluations of chemically distinct lychee cultivars are underway to provide confirmation of the specific kairomones utilized by female X. glabratus for host location and recognition.
Caption: Fig. 1. Behavioral bioassays were conducted in 4.4 liter buckets covered with cheese cloth and held within screened insect cages (A). Each bucket contained 10-15 female Xyleborus glabratus and one bolt of wood (B). Bolts were checked at regular intervals and females were considered fully committed to boring (scored positive) when they were perpendicular to the substrate and half their body length (~1 mm) was inserted into the entrance hole (C, female on left). Note: the female on right has chosen a site and initiated boring (in head-down posture), but would not be scored positive in our bioassay.
Caption: Fig. 2. Field infestation experiments were conducted with potted lychee trees (Litchi chinensis cv. 'Brewster'). One branch was pruned flush with the trunk, and this portion of the tree was then enclosed in a zippered mesh sleeve and 10 female Xyleborus glabratus were introduced (A). At 1 h, observations through the sleeve indicated that many beetles were walking over the freshly-cut surface (B). At 16 h, three beetles were actively boring into the cut surface (C). At 2 wk, four distinct entrance holes were observed on the tree, and fresh sawdust was present, indicative of active boring (D). (All photos of experimental Tree C.)
Caption: Fig. 3. Longitudinally dissected trunks from no-choice studies on potted lychee Trees C and D (Litchi chinensis cv. 'Brewster') in which galleries of Xyleborus glabratus have been exposed and indicated with "V's". Discolored tissue adjacent to the galleries was removed with a hammer and chisel and assayed for Raffaelea lauricola, as decribed in the text.
Caption: Fig. 4. Mean ([+ or -] SE) percentage of female Xyleborus glabratus boring into wood bolts presented in a 48-h no-choice bioassay. Treatments consisted of silkbay (Persea humilis), lychee (Litchi chinensis cvs. 'Brewster' and 'Mauritius'), and live oak (Quercus virginiana). Rate of boring with silkbay and lychee was best fit by regression analysis with sigmoidal models (see text).
Caption: Fig. 5. Representative GC-MS analyses of sesquiterpenes obtained by Super Q collections from rasped bark/ cambium of (A) silkbay (Persea humilis), (B) lychee (Litchi chinensis cv. 'Brewster'), and (C) live oak (Quercus virginiana). Peak identifications are as follows: 1 = [alpha]-cubebene, 2 = [alpha]-copaene, 3 = Unknown 1 (RI = 1404), 4 = Unknown 2 (RI = 1425), 5 = [beta]-caryophyllene, 6 = [alpha]-humulene, 7 = [delta]-cadinene, and 8 = calamenene.
The authors gratefully acknowledge Dale Stennett (USDA-ARS, Miami, Florida) for air-layering the large diam 'Brewster' trees used in the field infestation study; Jill Ploetz and Josh Konkol (UF-TREC, Homestead, Florida) for conducting laboratory and greenhouse assays with R. lauricola; Mike Winterstein (USDA-ARS, Miami, Florida) for assistance with the USDA-ARS germplasm collection; Nicole Ranalli (Florida Fish and Wildlife Conservation Commission) for permission to conduct field experiments at Royce Ranch, Lake Wales Ridge Wildlife Management Area (Lake Placid, Florida); Hilary Swain for providing laboratory space at Archbold Biological Station (Lake Placid, Florida); and the anonymous journal reviewers for helpful suggestions with the manuscript. This work was supported in part by the USDA-ARS National Plant Disease Recovery System, a NIFA Critical Issues Grant, and the Florida Avocado Administrative Committee. This report presents the results of research only; mention of a proprietary product does not constitute an endorsement by the USDA.
BRAR, G. S., CAPINERA, J. L., MCLEAN, S., KENDRA, P. E., PLOETZ, R. C., AND PENA, J. E. 2012. Effect of trap size, trap height, and age of lure on sampling Xyleborus glabratus (Coleoptera: Curculionidae: Scolytinae), and its flight periodicity and seasonality. Florida Entomol. 95: 1003-1011.
BRAR, G. S., CAPINERA, J. L., KENDRA, P. E., MCLEAN, S., AND PENA, J. E. 2013. Life cycle, development, and culture of Xyleborus glabratus (Coleoptera: Curculionidae: Scolytinae). Florida Entomol. 96: 1158-1167.
CARRILLO, D., DUNCAN, R. E., PLOETZ, J. N., CAMPBELL, A., PLOETZ, R. C., AND PENA, J. E. 2013. Lateral transfer of a phytopathogenic symbiont among native and exotic ambrosia beetles. Plant Pathol. Article first published online: 10 MAY 2013 | DOI: 10.1111/ppa.12073.
CRANE, J. H., BALERDI, C. F., AND MAGUIRE, I. 2008. Lychee growing in the Florida home landscape. <http://edistt.ifas.ufl.edu/mg051>
FDACS. 2012. Florida Department of Agriculture and Consumer Services identifies laurel wilt disease in avocado production area of Miami-Dade County, Press Release 1 May 2012. <http://www.freshfromflorida.cormnewsroom/ press/2012/05012012.html>
FRAEDRICH, S. W., HARRINGTON, T. C., RABAGLIA, R. J., ULYSHEN, M. D., MAYFIELD III, A. E., HANULA, J. L., EICKWORT, J. M., AND MILLER, D. R. 2008. A fungal symbiont of the redbay ambrosia beetle causes a lethal wilt in redbay and other Lauraceae in southeastern USA. Plant Dis. 92: 215-224.
FRAEDRICH, S. W., HARRINGTON, T. C., BATES, C. A., JOHNSON, J., REID, L. S., BEST, G. S., LEININGER, T. D., AND HAWKINS, T. S. 2011. Susceptibility to laurel wilt and disease incidence in two rare plant species, pondberry and pondspice. Plant Dis. 95: 1056-1062.
HANULA, J. L., AND SULLIVAN, B. 2008. Manuka oil and phoebe oil are attractive baits for Xyleborus glabratus (Coleoptera: Curculionidae: Scolytinae), the vector of laurel wilt. Environ. Entomol. 37: 1403-1409.
HANULA, J. L., MAYFIELD III, A. E., FRAEDRICH, S. W., AND RABAGLIA, R. J. 2008. Biology and host association of redbay ambrosia beetle, Xyleborus glabratus (Coleoptera: Curculionidae: Scolytinae), exotic vector of laurel wilt killing redbay (Persea borbonia) trees in the Southeastern United States. J. Econ. Entomol. 101: 1276-1286.
HANULA, J. L., SULLIVAN, B. T., AND WAKARCHUK, D. 2013. Variation in manuka oil lure efficacy for capturing Xyleborus glabratus (Coleoptera: Curculion-idae: Scolytinae), and cubeb oil as an alternative attractant. Environ. Entomol. 42: 333-340.
HARRINGTON, T. C., FRAEDRICH, S. W., AND AGHAYEVA, D. N. 2008. Raffaelea lauricola, a new ambrosia beetle symbiont and pathogen on the Lauraceae.
Mycotaxon 104: 399-404. HARRINGTON, T. C., AGHAYEVA, D. N., AND FRAEDRICH, S. W. 2010. New combinations of Raffaelea, Am-brosiella, and Hyalorhinocladiella, and four new species from the redbay ambrosia beetle, Xyleborus glabratus. Mycotaxon 111: 337-361.
HARRINGTON, T. C., YUN, H. Y., LU, S. S., GOTO, H., AGHAYEVA, D. N., AND FRAEDRICH, S. W. 2011. Isolations from the redbay ambrosia beetle, Xyleborus glabratus, confirm that the laurel wilt pathogen, Raffaelea lauricola, originated in Asia. Mycologia 103: 1028-1036.
HEATH, R. R., AND MANUKIAN, A. 1992. Development and evaluation of systems to collect volatile semiochemicals from insects and plants using a charcoalinfused medium for air purification. J. Chem. Ecol. 18: 1209-1226.
HEATH, R. R., MANUKIAN, A., EPSKY, N. D., SIVINSKI, J., CALKINS, C. O., AND LANDOLT, P. J. 1993. A bioassay system for collecting volatiles while simultaneously attracting tephritid fruit flies. J. Chem. Ecol. 19: 2393-2410.
IBRAHIM, M. A., EGIGU, M. C., KASURINEN, A., YAHYA, A., AND HOLOPAINEN, J. K. 2010. Diversity of volatile organic compound emissions from flowering and vegetative branches of Yeheb, Cordeauxia edulis (Caesalpiniaceae), a threatened evergreen desert shrub. Flavour Fragr. J. 25: 83-92.
KARIMURA, H., AND HIJII, N. 1994. Reproduction and resource utilization of the ambrosia beetle, Xylosandrus mutilatus, in field and experimental populations. Entomol. Exp. Appl. 71: 121-132.
KENDRA, P. E., MONTGOMERY, W. S., NIOGRET, J., PENA, J. E., CAPINERA, J. L., BRAR, G., EPSKY, N. D., AND HEATH, R. R. 2011. Attraction of the redbay ambrosia beetle, Xyleborus glabratus, to avocado, lychee, and essential oil lures. J. Chem. Ecol. 37: 932-942.
KENDRA, P. E., MONTGOMERY, W. S., NIOGRET, J., DEY-RUP, M. A., GUILLEN, L., AND EPSKY, N. D. 2012a. Xyleborus glabratus, X. affinis, and X. ferrugineus (Coloeptera: Curculionidae: Scolytinae): Electroan-tennogram responses to host-based attractants and temporal patterns in host-seeking flight. Environ. Entomol. 41: 1597-1605.
KENDRA, P. E., MONTGOMERY, W. S., SANCHEZ, J. S., DEYRUP, M. A., NIOGRET, J., AND EPSKY, N. D. 2012b. Method for collection of live redbay ambrosia beetles, Xyleborus glabratus (Coleoptera: Curculionidae: Scolytinae). Florida Entomol. 95: 513-516.
KENDRA, P. E., NIOGRET, J., MONTGOMERY, W. S., SANCHEZ, J. S., DEYRUP, M. A., PRUETT, G. E., PLOETZ, R. C., EPSKY, N. D., AND HEATH, R. R. 2012c. Temporal analysis of sesquiterpene emissions from manuka and phoebe oil lures and efficacy for attraction of Xyleborus glabratus (Coleoptera: Curculionidae: Scolytinae). J. Econ. Entomol. 105: 659-669.
KENDRA, P. E., MONTGOMERY, W. S., NIOGRET, J., AND EPSKY, N. D. 2013. An uncertain future for American Lauraceae: A lethal threat from redbay ambrosia beetle and laurel wilt disease (A review). American J. Plant Sci. 4: 727-738.
MAYFIELD III, A. E., AND BROWNIE, C. 2013. The red-bay ambrosia beetle (Coleoptera: Curculionidae: Scolytinae) uses stem silhouette diameter as a visual host-finding cue. Environ. Entomol. 42: 743-750.
MAYFIELD III, A. E., AND HANULA, J. L. 2012. Effect of tree species and end seal on attractiveness and utility of cut bolts to the redbay Ambrosia beetle and granulate ambrosia beetle (Coleoptera: Curculionidae: Scolytinae). J. Econ. Entomol.105: 461-470.
MAYFIELD III, A. E., CRANE, J. H., SMITH, J. A., PENA, J. E., BRANCH, C. L., OTTOSON, E. D., AND HUGHES, M. 2008. Ability of redbay ambrosia beetle (Coleoptera: Curculionidae: Scolytinae) to bore into young avocado (Lauraceae) plants and transmit the laurel wilt pathogen (Raffaelea spp.). Florida Entomol. 91: 485-487.
MAYFIELD III, A. E., MACKENZIE, M., CANNON, P. G., OAK, S. W., HORN, S., HWANG, J., AND KENDRA, P. E. 2013. Suitability of California bay laurel as a potential host for the non-native redbay ambrosia beetle and granulate ambrosia beetle (Coleoptera: Curculionidae: Scolytinae). Agric. Forest Entomol. 15: 227-235.
MIZUNO, T., AND KAJIMURA, H. 2002. Reproduction of the ambrosia beetle, Xyleborus pfeili (Ratzeburg) (Col., Scolytidae), on semi-artificial diet. J. Appl. Entomol. 126: 455-462.
NIOGRET, J., KENDRA, P. E., EPSKY, N. D., AND HEATH, R. R. 2011a. Comparative analysis of terpenoid emissions from Florida host trees of the redbay ambrosia beetle, Xyleborus glabratus (Coleoptera: Curculionidae: Scolytinae). Florida Entomol. 94: 1010-1017.
NIOGRET, J., MONTGOMERY, W. S., KENDRA, P. E., HEATH, R. R., AND EPSKY, N. D. 2011b. Attraction and electroantennogram responses of male Mediterranean fruit fly to volatile chemicals from Persea, Litchi, and Ficus wood. J. Chem. Ecol. 37: 483-491.
NIOGRET, J., EPSKY, N. D., SCHNELL, E. Q., SCHNELL, R. J., HEATH, R. R., MEEROW, A. W., AND KENDRA, P. E. 2013a. Analysis of sesquiterpene distributions in the leaves, branches, and trunks of avocado (Persea ameriana Mill.). American J. Plant Sci. 4: 922-931.
NIOGRET, J., EPSKY, N. D., SCHNELL, R. J., BOZA, E. J., KENDRA, P. E., AND HEATH, R. R. 2013b. Terpenoid variations within and among half-sibling avocado trees, Persea americana Mill. PLOS One 8: e73601.
PLOETZ, R. C., AND KONKOL, J. 2013. First report of gulf licaria, Licaria triandra, as a suscept of laurel wilt. Plant Dis. 97: 1248.
PLOETZ, R. C., PEREZ-MARTINEZ, J. M., SMITH, J. A., HUGHES, M., DREADEN, T. J., INCH, S. A., AND FU, Y. 2012. Responses of avocado to laurel wilt, caused by Raffalelea lauricola. Plant Pathol. 61: 801-808.
SINGH, G., MARIMUTHU, P., DE HELUANI, C. S., AND CATALAN, C. A. N. 2007. Chemical constituents, antioxidative and antimicrobial activities of essential oil and oleoresin of tailed pepper (Piper cubeba L.). Intl. J. Food Eng. 3: 1-22.
SYSTAT SOFTWARE. 2010. SigmaPlot 11.2 User's Guide. Systat Software, Inc., San Jose, CA.
USDA-FS. 2013. Laurel wilt distribution. United States Department of Agriculture, Forest Service, Forest Health Protection, Southern Region. <http://www. fs.fed.us/r8/foresthealth/laurelwilt/dist_map.shtml>
Paul E. Kendra (1), *, Randy C. Ploetz (2), Wayne S. Montgomery (1), Jerome Niogret (1), Jorge E. Pena (2), GURPREET S. BRAR (3) AND NANCY D. EPSKY (1)
(1) USDA-ARS, Subtropical Horticulture Research Station, Miami, FL 33158 USA
(2) University of Florida, Tropical Research and Education Center, Homestead, FL 33031 USA
(3) University of Florida, Department of Entomology and Nematology, Gainesville, FL 32611 USA
* Corresponding author; E-mail: firstname.lastname@example.org
TABLE 1. QUANTITY ([mu]G, MEAN [+ or -] S.D.) OF VOLATILE SESQUITERPENES EMITTED FROM WOOD BOLTS OF LYCHEE CV. 'BREWSTER' (LITCHI OHINENSIS), SILKBAY (PERSEA H'MILIS), AND LIVE OAK (QUERCUS VIRGINIANA). VOLATILES WERE ISOLATED FROM 6-G SAMPLES OF RASPED BARK-CAMBIUM BY SUPER Q COLLECTION, AND THEN ANALYZED BY GC-MS (DB-5MS COLUMN). Lychee cv. Peak Chemical RI (a) 'Brewster' 1 [alpha]-cubebene (b) 1361 0.1 [+ or -] 0.1 2 [alpha]-copaene (b) 1394 2.6 [+ or -] 3.2 3 Unknown 1 1404 0.0 [+ or -] 0.0 4 Unknown 2 1425 0.0 [+ or -] 0.0 5 [beta]-caryophyllene 1443 5.5 [+ or -] 6.4 (b) 6 [alpha]-humulene (b) 1478 1.1 [+ or -] 1.2 7 [delta]-cadinene (b) 1532 0.0 [+ or -] 0.0 8 calamenene (c) 1534 0.2 [+ or -] 0.2 Peak Silkbay Live Oak 1 7.5 [+ or -] 4.4 0.1 [+ or -] 0.1 2 12.3 [+ or -] 3.6 1.0 [+ or -] 0.3 3 2.9 [+ or -] 1.5 1.3 [+ or -] 0.3 4 6.3 [+ or -] 4.2 0.0 [+ or -] 0.0 5 25.4 [+ or -] 11.2 5.2 [+ or -] 0.3 6 2.1 [+ or -] 1.0 0.3 [+ or -] 0.1 7 1.5 [+ or -] 0.2 0.8 [+ or -] 0.3 8 4.8 [+ or -] 3.9 0.0 [+ or -] 0.0 (a) Mean Kovats Retention Index calculated from 3 replicates per species. (b) Identification by NIST/EPA/NIH mass spectral library (NIST11), then verified by comparison with RI of synthetic chemicals (see text). (c) Identification by NIST/EPA/NIH mass spectral library (NIST11), then verified by comparison with RI from published reports (see text).
Please note: Some tables or figures were omitted from this article.
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|Author:||Kendra, Paul E.; Ploetz, Randy C.; Montgomery, Wayne S.; Niogret, Jerome; Pena, Jorge E.; Brar, Gurp|
|Date:||Dec 1, 2013|
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