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Evaluation of cytogenotoxic and nutrient composition of three commonly consumed vegetables in South-Western Nigeria.

INTRODUCTION

Human diets in most developing countries have green leafy vegetables as one of the indispensable sources of carbohydrate, protein, vitamins and minerals, as well as roughages which promote digestion and prevent constipation [1, 2]. In Nigeria, most of the commonly eaten vegetables are the succulent leaves of herbaceous plants which are consumed as supplements, side dishes, prepared in soup as condiments or eaten with other major staple foods. Different parts, such as roots, stems, leaves, fruits or seeds of vegetables may be edible, and each of them contributes to diet in its own way [3]. Vegetable fats and oils have been reported to lower blood lipids, thereby reducing the occurrences of diseases associated with the damage of the coronary artery [4]. High fruit and vegetable diets have also been reported to have an ameliorating effect on the blood pressure of hypertensive patients [5], lowered the systolic and diastolic blood pressure, blood viscosity and plasma fibrinogen [6].

Corchorus olitorius (L.), Celosia argentea var. cristata (L.), and Ocimum gratissimum (L.) are among the commonly consumed vegetables in the South-West zone of Nigeria. C. olitorius is usually recommended for pregnant women and nursing mothers due to its richness in iron [7]. C. argentea is used as vegetable and ornamental plant and also, its dried leaves, inflorescences and seeds are used in traditional Chinese medicine [8]. Celogentins and moroidin, the bicyclic peptides isolated from the seeds of C. argentea, showed potent inhibition of tubulin polymerization, suggesting antimitotic activity of these compounds [9.10]. The genus Ocimum belongs to the economically important group of herbaceous plants widely distributed in tropical Africa and being grown in different parts of Nigeria. In African traditional medicine, the aqueous leaves decoction of O. gratissimum is used to treat gastrointestinal disorders and helminthiasis [11] and also has antioxidant properties [12]. Eugenol, an essential oil of O. gratissimum, demonstrated leishmanicidal activity and was not cytotoxic against mammalian cells [13]. However, in another report, Ocimum oil was reported to be capable of invoking an inflammatory response that transits from acute to chronic toxicity if continuously administered [14].

Although vegetables contain essential nutrients for human health benefits, some are toxic, or contain constituents that compromise digestion and absorption of vital nutrients [15]. The toxic constituents of vegetables may act on the chromosomes, thereby causing serious genetic problems such as cancers in the affected individuals or inheritance of abnormal genes in their offspring [16]. Proximate and mineral composition of the selected vegetables used in the present study have been reported in different locations around Ogbomoso, Nigeria [2, 17, 18] but none of the investigations included in vivo cyto-genotoxic evaluations. It is on this basis that the further screening of these common edible vegetables; C. olitorius, C. argentea and O. gratissimum for bioaccumulation of toxic minerals coupled with the genotoxicity of their aqueous extracts were conducted. This is with a view to give an insight into possible hazards associated with their consumption either as part of human diet or for medicinal purposes.

MATERIALS AND METHODS

Collection of samples

Three vegetables namely: Corchorous Olitorious (L.), Ocimum gratissimum (L.) and Celosia argentea var. cristata (L.) were freshly obtained from 'Wazo' market in Ogbomoso, Oyo state, Nigeria and were identified at the herbarium, LAUTECH, Ogbomoso.

Physicochemical analysis of water used: The water sample used for extracts preparation and planting of control onion bulbs was subjected to physicochemical analysis. The parameters evaluated included: pH, dissolved oxygen, total suspended solids, total dissolved solids, sulphate content, nitrate content, salinity, conductivity, biological oxygen demand and alkalinity [19]. The concentrations of the selected metals ([Cd.sup.2+], [Cr.sup.+2][Cu.sup.2+], [Fe.sup.2+], [Pb.sup.2+], [Mg.sup.2+], [Ni.sup.2+] and [Zn.sup.2+]) were also analyzed.

Preparation of extract

Five hundred grams (500g) each of the vegetables were weighed and boiled separately in 1L of borehole water. The aqueous extracts obtained were allowed to cool, after which the extracts were filtered and kept at 4[degrees]C for experimental use.

Proximate analysis of the three vegetables

Moisture, ash, protein, fibre, fat and carbohydrate contents of the studied vegetables were determined using the standard method [20]. All the proximate values were calculated and recorded [2, 21].

Metal contents analysis of the three vegetables

The presence and concentrations of [Cd.sup.2+], [Cr.sup.+2][Cu.sup.2+], [Fe.sup.2+], [Pb.sup.2+], [Mg.sup.2+], [Ni.sup.2+] and [Zn.sup.2+] in the three vegetables were determined by wet digestion and analyzed using Atomic Absorption Spectrometer--AAS (PerkinElmer A Analyst 100). Standards for the analyzed metals were prepared from a known concentration of 1000 [micro]g/ml [21]. The samples analyses were carried out following the appropriate quality assurance procedures and precautions in order to ensure reliability of the data [2].

Allium cepa assay

Onions bulbs (Allium cepa) were purchased from 'Wazo' market in Ogbomoso, Nigeria and sun dried for three weeks before being used for the experiment. The dried outer scales and roots in the onions were removed carefully, leaving the primordial root ring intact. The stock extract was diluted using the same water that was used for the control group into seven concentrations; 0.5, 1.0, 2.5, 5.0, 10.0, 25.0 and 50.0 ppt. Ten onion bulbs were planted per concentration of each of the extracts in a dark cupboard at room temperature. After 24 h, fresh extract solution was prepared and the previously suspended onions were transferred thereafter for the root growth to continue [22, 23].

Microscopic evaluation

After 48 h of root growth, root tips from five out of ten onions per concentration were cut, fixed separately in ethanol-ethanoic acid (ratio 3:1) fixative and stored at 4[degrees]C for slide preparation. The fixed root tips were first hydrolyzed in 1N HCl and further treated as previously described [22]. Two root tips were teased on a clean glass slide using a pair of needles. One--two drops of aceto-orcein stain was added to the homogenized root tips and left for 10 minutes. Thereafter, excess stain was removed with a filter paper and a cover slip was carefully placed on the slide. The slide was wrapped with rolls of tissue paper and tapped with an index finger to remove excess stain, after which the edges of the cover slip were then sealed with nail polish. Five slides were prepared and scored per concentration. A total of 5000 cells per concentration were examined for dividing cells and chromosomal aberrations using oil immersion objective lens. Mitotic index and frequency of chromosomal aberrations were calculated as reported [23].

Macroscopic evaluation

Root lengths from the remaining five onions per concentration were measured after 72 h. The average root lengths obtained for the treated and control groups were calculated and used for plotting a graph of percentage root length against concentration (22). Therefore, the level of toxicity of aqueous extracts on the root growth in A. cepa was determined from the graph.

Statistical method

The data obtained from proximate and metal analyses, as well as the root lengths, mitotic indices and frequency of chromosomal aberrations of the treated groups and the control were compared using one way analysis of variance (ANOVA) and Duncan's multiple range test. The difference between the control and treated groups was considered significant at p [less than or equal to] 0.05. All statistical analyses were performed with SPSS software, version 15.0.

RESULTS

The proximate analyses of the studied vegetables (Table 1) showed that carbohydrate was the most abundant with the value obtained for C. argentea (31.41%) significantly lower compared to other two vegetables. The values of protein (30.79%), ash (19.98), and fat (0.22%) obtained for C. argentea were significantly different from those obtained for O. gratissimum and C. olitorius vegetables, while the highest moisture content (8.85%) was found in O. gratissimum.

The concentrations of Magnesium and Zinc in C. argentea were significantly different from the other two vegetables. There were no significant differences in the values of Iron in C. olitorius and C. argentea both of which were 20.86 mg/l, and values of lead obtained for O. gratissimum (0.21mg/l) and C. argentea (0.20 mg/l). Cadmium and Nickel were not detected in the three vegetables (Table 2). The physicochemical analysis of the bore-hole water used as control and diluent for A. cepa assay is shown in table 3. The values obtained for Zn (0.248 mg/l) and Fe (0.26 mg/l) in the bore-hole water were lower compared to values obtained for the vegetables extracts while Cd, Cr, Pb, Ni and Cu were not detected.

The toxicity of aqueous extracts of C. olitorius, O. gratissimum and C. argentea to the root growth of A. cepa is represented in Figure 1. The values obtained for [EC.sub.50] were 6.3, 19.0 and 22.4 ppt for C. argentea, O. gratissimum and C. olitorius, respectively. The percentage root growth inhibitions caused by the aqueous extracts of the three vegetables at 5.0, 10, 25 and 50 ppt was significantly different (p [less than or equal to] 0.05) from the control.

Figure 2 shows the activity of the aqueous extracts of C. olitorius, O. gratissimum and C. argentea in suppressing mitosis in the root tips of A. cepa. Significant reduction in the number of dividing cells was observed at all concentrations of C. argentea whereas, the significant inhibition of mitosis occurred at 25 and 50 ppt of C. olitorius and 2.5, 10, and 50 ppt of O. gratissimum. Inhibition of mitosis in A. cepa by the aqueous extracts of C. olitorius and O. gratissimum was not concentration dependent, unlike the mitotic inhibitory effect of aqueous extract of C. argentea, which was dose dependent from 2.5 ppt to 50 ppt.

The aqueous extracts of C. olitorius, O. gratissimum and C. argentea induced the following chromosomal aberrations; c-mitosis, chromosome bridge and sticky chromosome, in the A. cepa root tip cells (Table 3). The frequencies of the aberrations; 0.20% and 0.14% recorded at 1.0 ppt and 25.0 ppt, respectively for the aqueous extract of O. gratissimum, were significantly different from the control, while no chromosomal aberration was induced at 50.0 ppt of the extract. Similarly, no chromosomal aberration was induced at the first four concentrations of C. argentea, while those aberrations induced at the last three concentrations of C. argentea were not significantly different from the control, which had no aberration. The induced chromosomal aberrations by these vegetables were not dose-dependent.

DISCUSSION

Green leafy vegetables provide humans with adequate amounts of many vitamins and minerals and thus occupy an important place among the food crops. They are valuable in maintaining alkaline reserve of the body because of their nutrient and mineral contents [2]. Among the nutrient and mineral contents analyzed in this study, the percentage of carbohydrate and protein in the three vegetables ranged from 31.42 49.39% and 22.24-30.79%, respectively. The results are higher than the carbohydrate and protein contents of C. olitorius and C. argentea reported [2]. The protein contents of the three vegetables are within the range of 20.48-41.66% reported as the protein level of green leafy vegetables [25]. Plant food that provides more than 12% of its calorific value from protein is considered good source of protein [26]. Therefore, these results suggest that C. olitorius, O. gratissimum and C. argentea are good source of protein and carbohydrate for humans, especially in developing countries where most people depend on starch-based food as the main staple food for the supply of both energy and protein [2]. The moisture (7.16-8.85%) and ash (10.79-19.98%) contents of these vegetables are lower than the values reported for C. olitorius, C. argentea [2, 18] and O. gratissimum [27]. The difference in the sampling time and location of vegetables may be responsible for the disparity in the results of moisture contents. The fibre contents of C. olitorius (7.40%) and C. argentea (10.22%) are almost similar to 6.70% and 11.70% previously obtained for these two vegetables, respectively [2]. Dietary fibre has been reported to lower the risk of coronary heart disease, hypertension, constipation, diabetes, and colon and breast cancer [28, 29]. Different fat content from the one obtained in this study was earlier reported for C olitorius [2]. O. gratissimum had the least fat content of 0.09%. Similarly, the lowest values of fat content among the analyzed nutrients in the vegetables studied had been observed in Amaranthus cruentus, Celosia argentea and Corchorus olitorius leaves [2]. The results of proximate analysis on these three vegetables showed that they could be good for health by providing most of the essential nutrients for normal body functions when consumed in appropriate combinations.

The highest magnesium content, followed by iron, suggests health benefits of the vegetables to their consumers in terms of cellular metabolism and protein synthesis. However, the levels of [Pb.sup.2+] (0.14-0.21 mg/L) which are lesser than the suggested concentration of 2-6 mg/l in the plant species [30], as well as the lack of [Cd.sup.2+] and [Ni.sup.2+], corroborates the use of these vegetables as food supplement. The observed values for the metals evaluated could only have been from bioaccumulation by the vegetable since the physicochemical analysis of the water especial in metal content were lower compared to those of the vegetables and were within the permissible levels of Nigerian Standard for Drinking water Quality (24).

The activity of aqueous extracts of the vegetables on the root growth of A. cepa shows inhibitory effects as indicated by the significant (p [less than or equal to] 0.05) root growth inhibition observed at 5.0, 10.0, 25.0 and 50.0 ppt of the three vegetables. The aqueous extract of C. agentea demonstrated highest root growth inhibitory effect, as indicated by its lowest [EC.sub.50] value. The root growth inhibitory activities of the aqueous extracts in this study might be due to the presence of some heavy metals. Heavy metals such as Zinc, Copper and Lead at different concentrations have been implicated in inhibition of root growth in Allium cepa [31, 32]. Similar root growth inhibitory effects of aqueous extracts of Vernonia amygdalina, Amaranthus caudatum and Telfairia occidentalis in A. cepa have been reported [22].

The significant (p [less than or equal to] 0.05) reduction in values of mitotic index obtained at various concentrations of the vegetables compared to the control suggests mitodepression. The concentration-dependent (1.0-50.0 ppt) inhibition of mitosis in the root tips of A. cepa by the aqueous extract of C. argentea is an indication of cytotoxicity, whereas the antimitotic activity of the aqueous extracts of C. olitorius and O. gratissimum was less, and could be regarded as cytostatic because of their non-dose related suppression of mitosis. Celogentins and moroidin are bicyclic peptides isolated from the seeds of C. argentea, and have been implicated in the inhibition of microtubule formation, hence, arrest of mitosis in eukaryotic cells [9, 10]. Therefore, the presence of these phytochemicals in the aqueous extract of C. argentea may be responsible for the inhibition of mitosis in A. cepa cells as observed in this study. This corroborates the higher toxicity to root growth of A. cepa, by the extract of C. argentea compared to the aqueous extracts of the other two vegetables. It has been observed that whenever there is root growth inhibition in the A. cepa, there is always reduction in the number of dividing cells [23, 32, 33].

The induced chromosomal aberrations by the aqueous extracts of these vegetables were not concentration dependent except at 10.0, 25.0 and 50.0 ppt of C. argentea. Although aqueous extracts of O. gratissimum induced chromosomal aberrations at 1.0 and 25.0 ppt concentrations which were significantly different from control, yet, its mutagenic effects as well as that of C. olitorius could be considered weak on the basis of their induction of chromosomal aberrations in a non-dose related manner. However, the potency of the aqueous extract of C. argentea in inducing chromosomal aberrations in A. cepa cells may be significant and more worrisome at higher concentrations than those used in this study. The ability of celogentins and moroidin in inhibiting the polymerization of tubulin has been reported in cells [9, 10]. This may disallow the assemblage of sipindle fibres, thereby causing chromosomal aberrations. Occurrence of these aberrations in A. cepa indicate that similar observations might be possible in higher eukaryotic organisms, human inclusive, if exposed to substantial concentration since the basic structure of the DNA are the same in all organisms.

CONCLUSIONS

This study revealed that C. olitorius, O. gratissimum and C. argentea are nutritious and good for human consumption, as they contain various classes of food, without possessing harmful levels of heavy metals. However, it is worth mentioning that the phytochemicals in these vegetables could significantly suppress cell division in eukaryotic cells, and may induce chromosomal aberrations at higher concentrations. Nonetheless, the antimitotic potential of the extract of C. argentea may favour its uses in the development of drugs to prevent the uncontrolled proliferation of cancer cells of which further investigation is required.

Acknowledgement: The authors appreciate the effort of Dr. A.T. J. Ogunkunle of Department of Pure and Applied Biology, Ladoke Akintola University of Technology, Ogbomoso for assistance in identifying the plants used for the study.

REFERENCES

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[30] Broyer TC, Johnson CN and R Pull Some aspects of lead in plant nutrition. Plant Soil 1972; 36: 301.

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[32] Fiskesjo G Allium test for screening chemicals; evaluation of cytologic parameters. In: Wang, W., Gorsuch, J.W., Hughes, J.S. (Eds), Plants for Environmental Studies. CRC Lewis Publishers, Boca Raton, New York, 1997; 308-333.

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Yekeen TA (1) *, Akintaro OI (1), Akinboro A (1) and MA Azeez

* Corresponding author email: tayekeen@yahoomail.com

(1) department of Pure and Applied Biology, Ladoke Akintola University of Technology, Ogbomoso, Nigeria

Table 1: Nutritional components (%) of Corchorus olitorius, Ocimum
gratissimum and Celosia argentea used for the study

Vegetable       Moisture     Ash          Protein

C. olitorius    7.16 [+ or   11.97 [+     25.18 [+
                -] 0.08      or -] 0.52   or -] 2.12
                             (b)          (b)

O.gratissimum   8.85 [+ or   10.79 [+     22.24 [+
                -] 0.12      or -] 0.82   or -] 1.75
                             (b)          (b)

C. argentea     7.38 [+ or   19.98 [+     30.79 [+
                -] 0.05      or -] 1.04   or -] 2.72
                             (a)          (a)

Vegetable       Fibre        Fat          Carbohydrate

C. olitorius    7.40 [+ or   0.12 [+ or   48.17 [+
                -] 0.02      -] 0.03      or -] 2.23
                             (b)          (a)

O.gratissimum   8.64 [+ or   0.09 [+ or   49.39 [+
                -] 0.61      -] 0.01      or -] 1.07
                             (b)          (a)

C. argentea     10.22 [+     0.22 [+ or   31.41 [+
                or -] 0.42   -] 0.06      or -] 1.92
                             (a)          (b)

Data were presented using mean  [+ or -]  Standard error of mean

Mean value with different alphabet showed significant difference
(p < 0.05)

Table 2: Analyzed metals in Corchorous olitorius, Ocimum gratissimum
and Celosia argentea used for the study

Vegetable      [Cd.sup.2+]   [Cu.sup.2+]   [Fe.sup.2+]   [Pb.sup.2+]

C. olitorius   ND            0.42 [+ or    20.86 [+ or   0.14 [+ or
                             -] 0.02       -] 1.73 (a)   -] 0.00 (b)

O. gratissium   ND           0.67 [+ or    10.76 [+ or   0.21 [+ or
                             -] 0.00       -] 0.98 (b)   -] 0.04 (a)

C. argentea    ND            0.57 [+ or    20.86 [+ or   0.20 [+ or
                             -] 0.01       -] 1.20 (a)   -] 0.01 (a)

Vegetable      [Mg.sup.2+]   [Ni.sup.2+]   [Zn.sup.2+]

C. olitorius   215.33 [+     ND            1.83 [+ or

               or -] 15.76                 -] 0.12 (b)
               (a)

O. gratissium  184.21 [+     ND            1.75 [+ or
               or -] 8.86                  -] 0.02 (b)
               (b)

C. argentea    226.40 [+     ND            2.57 [+ or
               or -] 12.56                 -] 0.17 (a)
               (a)

ND: Non detectable; Unit of measurement: mg/L

Mean value with different alphabet showed significant difference
(p < 0.05)

Table 3: Physicochemical characteristics of bore-hole water used for
the preparation of vegetable extracts and A. cepa root growth
solutions

Parameters          pH         DO       BOD       Alkalinity   TDS

Bore hole water     6.97       6.8      2.67      67.58        108
                    [+ or      [+ or    [+ or     [+ or -]     [+ or
                    -] 0.1     -] 0.4   -] 0.04   1.02         -] 6.9

NSDWQ (2007) (24)   6.5-8.5    --       --        --           500

Parameters          TSS       TS       S[O.sub.4.   N[O.sub.3.   Cd
                                       sup.2-]      sup.-]

Bore hole water     74        182      4.13         3.86         ND
                    [+ or     [+ or    [+ or -]     [+ or -]
                    -] 8.08   -] 9.2   0.15         0.04

NSDWQ (2007) (24)   --        --       100          50           0.003

Parameters          Cr     Pb     Ni     Cu    Zn        Fe

Bore hole water     ND     ND     ND     ND    0.248     0.26
                                               [+ or     [+ or
                                               -] 0.01   -] 0.02

NSDWQ (2007) (24)   0.05   0.01   0.02   1.0   3.0       0.3

Unit: mg/l except pH.

DO: Dissolved oxygen; BOD: Biological Oxygen Demand; TDS: Total
Dissolved Solid; TSS: Total Suspended Solid, TS: Total Solid, Cd:
Cadmium, Cr: Chromium, Pb: Lead, Ni: Nickel, Zn: Zinc, Fe: Iron

Table 4: Types and frequencies of chromosomal aberrations induced by
aqueous extracts of the three vegetables used for the study

Concentration    C-        Chromosome   Sticky
                 mitosis   bridge       chromosome

Control          0         0            0
0.5 ppt
C. olitorius     0         1            0
O. gratissimum   5         0            0
C. argentea      0         0            0
1.0 ppt
C. olitorius     0         0            0
gratissimum      6         0            4
C. argentea      0         0            0
2.5 ppt
C. olitorius     0         0            0
gratissimum      4         0            0
C. argentea      0         0            0
5.0 ppt
C. olitorius     0         0            0
gratissimum      4         0            0
C. argentea      0         0            0
10.0 ppt
C. olitorius     1         1            0
gratissimum      0         0            0
C. argentea      1         0            0
25.0 ppt
C. olitorius     0         0            0
gratissimum      5         2            0
C. argentea      1         0            0
50.0 ppt
C. olitorius     1         2            0
gratissimum      0         0            0
C. argentea      0         0            2

Concentration    Total         Frequency
                 aberrations   of aberrant
                               cells

Control          0             0.00
0.5 ppt
C. olitorius     1             0.02
O. gratissimum   5             0.10
C. argentea      0             0.00
1.0 ppt
C. olitorius     0             0.00
gratissimum      10            0.20 *
C. argentea      0             0.00
2.5 ppt
C. olitorius     0             0.00
gratissimum      4             0.08
C. argentea      0             0.00
5.0 ppt
C. olitorius     0             0.00
gratissimum      4             0.08
C. argentea      0             0.00
10.0 ppt
C. olitorius     2             0.04
gratissimum      0             0.00
C. argentea      1             0.02
25.0 ppt
C. olitorius     0             0.00
gratissimum      7             0.14 *
C. argentea      1             0.02
50.0 ppt
C. olitorius     3             0.06
gratissimum      0             0.00
C. argentea      2             0.04

* Values significantly different from control
(p [less than or equal to] 0.05)
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Author:Yekeen, T.A.; Akintaro, O.I.; Akinboro, A.; Azeez, M.A.
Publication:African Journal of Food, Agriculture, Nutrition and Development
Article Type:Report
Geographic Code:6NIGR
Date:Apr 1, 2013
Words:4665
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