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Evaluation of [sup.99m]Tc-HYNIC-[beta]Ala-[Bornbesin.sub.(7-14)] as an agent for pancreas tumor detection in mice.

Introduction

Cancer is one of the main causes of death worldwide. In 2012, about 14.1 million new cases of the disease were registered, resulting in approximately 8.2 million deaths. Pancreatic adenocarcinoma has a high mortality rate. Global data showed 337,872 new cases of this disease in 2012, followed by 330,372 deaths, which represent approximately 98% of the cases (1). Deaths could be delayed if an early diagnosis could be achieved.

Cancer cells are characterized by the pathological upregulation of several physiological processes, including the overexpression of a variety of peptide receptors in cancer cell membranes (2). This upregulation enables the use of radiolabeled peptides for the differentiation between tumor and normal tissues by molecular imaging. For example, pancreas, prostate, lung, colon and breast cancer cells present an increased expression of gastrin-releasing peptide receptors (GRPr). Thus, a radiolabeled peptide derivative of gastrin-releasing peptide (GRP) may be used as a specific imaging probe for these types of tumors (3-5).

The neuropeptide bombesin consists of 14 amino acids and is analogous to GRP, differing in only 1 of 10 carboxy-terminal residues. Bombesin has been evaluated as a radiotracer for GRPr-overexpressing tumors (6-8). A truncated sequence of bombesin containing the eight carboxy-terminal residues ([Bombesin.sub.(7-14)]) necessary to retain its affinity for GRPr has been employed in order to identify tumors. Coincidentally, the removal of the six nitrogen-terminal residues also increases its stability (9,10).

Technetium-99m ([sup.99m]Tc) is the most widely used radionuclide in nuclear medicine since it presents suitable characteristics fora radioisotope, including a physical half-life of 6.02 h and gamma emission of low energy (140 keV) (11,12). For using [sup.99m]Tc for radiolabeling [Bombesin.sub.(7-14)], which does not have a disulfide bond, 2-hydrazinonicotinamide (HYNIC) is a good chelating agent because its carboxylic acid group reacts with the nitrogen-terminal residue of the peptide. This addition does not affect [Bombesin.sub.(7-14)] binding to GRPr, since it occurs near the carboxy-terminal residue. Moreover, a spacer amino acid, such as beta-alanine ([beta]Ala), can be added between HYNIC and [Bombesin.sub.(7-14)] without compromising the peptide's interaction with its receptor. Finally, the presence of co-ligands such as tricine and ethylenediamine-N,N'-diacetic acid (EDDA) in the radiolabeling procedure of HYNIC-[beta]Ala-[Bombesin.sub.(7-14)] stabilizes the metal complex (13,14).

The Capan-1 cell line was isolated from a liver metastasis of a human pancreatic adenocarcinoma. This tumor cell line is of ductal origin and exhibits characteristics of a well-differentiated adenocarcinoma. In addition, it produces a well-defined tumor nodule after subcutaneous inoculation in nude mice, mimicking the tumor in humans (15). It has been reported that this ductal cell line expresses functional GRPr on its membrane surface (16-18). Therefore, a GRP analog such as a Bombesin derivative may be used to identify Capan-1 tumor tissues.

The objective of the present study was to develop pancreatic adenocarcinomas in nude mice using the Capan-1 cell line and to evaluate the feasibility of [sup.99m]Tc-HYNIC-[beta]Ala-[Bombesin.sub.(7-14)] as an imaging probe for Capan-1 pancreatic adenocarcinoma.

Material and Methods

Material

The peptide HYNIC-[beta]Ala-[Bombesin.sub.(7-14)] was purchased from GL Biochem Ltd. (China). [sup.99m]Tc was obtained from a [sup.99]Mo/[sup.99m]Tc generator supplied by Instituto de Pesquisas Energeticas e Nucleares (IPEN; Brazil). Other reagents and solvents were acquired from Sigma-Aldrich (Brazil). The Capan-1 human cell line, Iscove's Modified Dulbecco's Medium (IMDM) and fetal bovine serum were purchased from American Type Culture Collection (ATCC; USA). Trypsin was acquired from Invitrogen (Brazil). Nude male BALB/c mice (18-20 g) were supplied by Fundacao de Apoio e Fomento a Inovacao Tecnologica, a Pesquisa e ao Ensino of IPEN (Brazil). Animals were kept under specific pathogen-free conditions, with ad libitum access to chow and water. The housing was temperature-controlled with Altered air and a light-dark cycle (12/12 h). The experiments were conducted according to animal-use principles approved by the local Ethics Committee on Animal Use of the Universidade Federal de Minas Gerais (CEUA/UFMG).

Radiolabeling of HYNIC-[beta]Ala-[Bombesin.sub.(7-14)]

The radiolabeling procedure was performed according to a method published elsewhere (19). Briefly, 20 mg of tricine and 5 mg of EDDA were added to a sealed vial and dissolved with 0.5 mL of 0.9% NaCl (w/v). To this solution, 10 [micro]g of HYNIC-[beta]Ala-[Bombesin.sub.(7-14)] and 10 [micro]L of a 1-mg/mL Sn[Cl.sub.2] x 2[H.sub.2]O solution in 0.25 N HCl were added and vacuum was applied. The pH was adjusted to 7-8, and 74 MBq of Na[sup.99m]Tc[O.sub.4] was added to the vial. Finally, the solution was heated in a water bath (100[degrees]C) for 15 min and cooled to room temperature. The specific activity of the final product was 8.5 MBq/nmol.

Radiolabeling yield

Radiolabeling yields were determined by thin-layer chromatography (TLC) on silica gel strips (Merck[R], Germany). Methyl ethyl ketone was used to determine the free technetium ([sup.99m]Tc[O.sup.-.sub.4]) and a solution of acetonitrile:water (1:1) was used to quantify hydrolyzed technetium ([sup.99m]Tc[O.sub.2]). Radioactivity was measured using an automatic gamma counter (Wizard, Finland).

Capan-1 cell culture

Capan-1 cells were maintained at 37[degrees]C in a humidified atmosphere containing 5% C[O.sub.2] and were continuously grown in IMDM supplemented with 10% (v/v) fetal bovine serum (FBS) (Sigma-Aldrich, USA), 100 IU/mL penicillin (Sigma-Aldrich), and 10 mg/mL streptomycin (Sigma-Aldrich). The cells were grown to confluence and then harvested by trypsinization. After centrifugation (5 min at 241 g), cells were re-suspended in IMDM for development of the pancreas tumor in an animal model.

Pancreas tumor animal model

An aliquot (100 [micro]L) containing 5 x [10.sup.6] Capan-1 cells in IMDM was injected subcutaneously in the right upper flank of each nude mouse. Tumors were allowed to grow in vivo for 25 days after the inoculations, with a tumor diameter of no more than 10 mm being obtained at this time. Capan-1 tumor-bearing mice were then used for ex vivo biodistribution studies and scintigraphic images.

Ex vivo biodistribution studies

Aliquots containing [sup.99m]Tc-HYNIC-[beta]Ala-[Bombesin.sub.(7-14)] (7.4 MBq) were administered into the tail vein of Capan-1 tumor-bearing mice (n = 5). After 4 h of radiolabeled peptide administration, the mice were anesthetized with a solution of 80 mg/kg ketamine and 15 mg/kg xylazine, and then euthanized. Organs and tissues of interest, such as spleen, heart, stomach, liver, small intestine, contralateral muscle, kidneys, blood, and tumor were removed and weighed. The associated radioactivity was determined with an automatic gamma counter (Wizard, Finland). Results are reported as percent of the injected dose per gram of tissue (% ID/g).

Histopathological analysis

After performing ex vivo biodistribution studies, Capan-1 tumor tissues were fixed in formalin (10% w/v in phosphate-buffered saline, pH 7.4), and sections (4 mm) were prepared for light microscopy studies. All staining procedures were performed on paraffinembedded sections mounted on glass slides. Histological section images were captured by a digital camera (Spot Insight Color, USA) adapted to an Olympus microscope (BX-40; Japan). SPOT[R] software (version 3.4.5) and CorelDRAW[R] (version 11.633, USA) were used for image analysis.

Scintigraphic images

[sup.99m]Tc-HYNIC-[beta]Ala-[Bombesin.sub.(7-14)] (7.4 MBq) was administered into the tail vein of Capan-1 tumor-bearing mice. At 1 and 4 h after injection, the mice were anesthetized with 80 mg/kg ketamine and 15 mg/kg xylazine, and then placed in a prone position under a gamma camera (Mediso, Hungary) with a low-energy high-resolution collimator. Images were acquired using a 256 x 256 x 16 matrix size with a 20% energy window set at 140 keV for 10 min. We chose an early time (1 h) and a late time (4 h) in order to demonstrate the clearance of the radiolabeled peptide. Regions of interest (ROIs) were analyzed by scintigraphic images outlining the tumor (target). The ROIs were automatically copied to the contralateral muscle (non-target). The target/non-target ratios were calculated using the total ROI counts.

Statistical analysis

Data are reported as means [+ or -] SD. The means of the 2 groups of ROIs (target and non-target) were compared using Student's t-test. P values of less than 0.05 were considered to be significant. Data were analyzed using the Prism software (version 5.00, USA).

Results and Discussion

Radiolabeling of HYNIC-[beta]Ala-[Bombesin.sub.(7-14)] and radiolabeling yield evaluation

HYNIC-[beta]Ala-[Bombesin.sub.(7-14)] was successfully labeled with [sup.99m]Tc, showing a radiolabeling yield of 97.72 [+ or -] 0.73% (n = 6). This yield is consistent with other works published by our group (19-21).

The presence of radiochemical impurities has proved to be a drawback in nuclear medicine, yielding images of poor quality. It is accepted that, to be injected, a radiopharmaceutical must present a radiolabeling yield higher than 90%. Therefore, the [sup.99m]Tc-HYNIC-[beta]Ala-[Bombesina.sub.(7-14)] complex presented good chemical characteristics, since it showed high radiochemical purity (>95%).

Histopathological analysis

A xenographic pancreatic adenocarcinoma was developed by the subcutaneous inoculation of the human ductal Capan-1 cell line in the right upper flank of nude male BALB/c mice. In order to obtain a suitable tumor focus, cells were allowed to grow for about 25 days after their inoculation into the animals. Histopathological examination (Figure 1) revealed that tumor tissue presented a delicate conjunctive stroma and more necrosis in the central area. Histopathological analysis also showed evident mitotic activity and the presence of papillary projections and granular cells.

Scintigraphic images and ex vivo biodistribution studies

Ex vivo biodistribution (Figure 2) showed that [sup.99m]Tc-HYNIC-[beta]Ala-[Bombesin.sub.(7-14)] presented high kidney uptake. Scintigraphic images (Figure 3) corroborate the biodistribution results, showing high radioactivity accumulation in the abdominal region, which was mainly due to [sup.99m]Tc-HYNIC-[beta]Ala-[Bombesin.sub.(7-14)] depuration, showing high levels of radioactivity in kidneys and bladder. These results indicate radiopeptide elimination by the urinary tract, which is consistent with the hydrophilic nature of the molecule (20-22). In addition, low levels of radioactivity were observed in stomach, liver, and spleen, corresponding to the low quantities of radiochemical impurities, such as [sup.99m]Tc[O.sup.-.sub.4] and [sup.99m]Tc[O.sub.2].

The xenographic pancreas tumor was successfully developed and identified 30 days after the subcutaneous inoculation of the Capan-1 human cell line into nude male BALB/c mice, as can be clearly seen by the scintigraphic images obtained 1 and 4 h after radiopeptide administration (Figure 3). Scintigraphic images showed higher uptake by the tumor (right thigh) when compared with the left thigh used as control. The tumor-to-muscle (T/M) ratio obtained by means of scintigraphic images (Figure 4) increased at 4 h (4.11 [+ or -] 0.75) compared to the values detected at 1 h (2.29 [+ or -] 0.13). This increase may be explained by a higher clearance of [sup.99m]Tc-HYNIC-[beta]Ala-[Bombesin.sub.(7-14)] from blood and contralateral muscle compared to its elimination from the tumor focus. The visual analysis of scintigraphic images corroborated the quantitative data, since the tumor focus was more evident at 4 h than at 1 h after radiopeptide injection into Capan-1 tumor-bearing mice. This finding was due to an increase in the target/nontarget ratio due to clearance of the radiopharmaceutical in the non-target organ.

Ex vivo biodistribution data showed that [sup.99m]Tc-HYNIC-[beta]Ala-[Bombesin.sub.(7-14)] uptake by the tumor focus was 0.47 [+ or -] 0.20% ID/g and demonstrated T/M and tumor-to-blood (T/B) of 4.42 [+ or -] 0.85 and 2.02 [+ or -] 0.36, respectively, at 4 h after radiopeptide administration. These results were similar to those obtained in a previous study by our group (19) in which an uptake of 0.39 [+ or -] 0.03% ID/g was achieved in Ehrlich tumor-bearing mice, reaching T/M and tumorto-blood (T/B) ratios of 4.78 [+ or -] 0.81 and 3.53 [+ or -] 0.53, respectively, at the same 4 h post injection. All target/non-target ratios from scintigraphic images and ex vivo biodistribution assay were higher than 1.5, which represents a 50% higher radiopeptide uptake by the target tissue than the by nontarget tissues. Thus, the radiotracer is able to produce high quality images (23), as can be seen in the present study where [sup.99m]Tc-HYNIC-[beta]Ala-[Bombesin.sub.(7-14)] was able to identify the Capan-1 focus at an earlier stage of tumor development.

Several published studies have reported in vivo profiles of [sup.99m]Tc-HYNIC-[beta]Ala-[Bombesin.sub.(7-14)] similar to those obtained in the present study. The authors described radio-peptide accumulation in the kidneys, bladder, and tumor sites after its administration in murine breast (Ehrlich cells) (19), human breast (MDA-MB-231 cells) (24), prostate (PC3 and LNCaP cells) (21,22) and colon (HT-29 cells) (25) tumor-bearing mice. However, to the best of our knowledge, no previous study has evaluated the feasibility of [sup.99m]Tc-HYNIC-[beta]Ala-[Bombesin.sub.(7-14)] to identify Capan-1 pancreatic adenocarcinoma.

The aggressiveness and high incidence of metastases associated with pancreatic adenocarcinoma represent a challenge for clinicians and researchers. A strategy to overcome some of these difficulties would be the early identification of the tumor. Genetic study of pancreatic cancer suggested an opportunity for the early detection of pancreatic neoplasia. However, this approach has some disadvantages since it is an invasive and an expensive methodology requiring a biopsy and expensive biochemical analysis, such as RT-PCR. Imaging methods for pancreatic tumors are innovative and rarely reported in the literature (26). Finally, the data presented here suggest that [sup.99m]Tc-HYNIC-[Bombesin.sub.(7-14)] could be used as a new potential agent to detect Capan-1 tumors by scintigraphic images at an early stage of tumor development. In addition, it could be used to monitor cancer therapy.

Acknowledgments

The research was supported by CNPq, Comissao Nacional de Energia Nuclear (CNEN), FAPEMIG, and Pro-Reitoria de Pesquisa da Universidade Federal de Minas Gerais (PRPq/UFMG).

References

(1.) Ferlay J, Soerjomataram I, Ervik M, Dikshit R, Eser S, Mathers C, et al. Cancer incidence and mortality worldwide: Sources, methods and major patterns in GLOBOCAN 2012. Int J Cancer 2015; 136: E359-E386, doi: 10.1002/ijc. 29210.

(2.) Schottelius M, Wester HJ. Molecular imaging targeting peptide receptors. Methods 2009; 48: 161-177, doi: 10.1016/j.ymeth.2009.03.012.

(3.) Ferro-Flores G, Ramirez FM, Melendez-Alafort L, Santos-Cuevas CL. Peptides for in vivo target-specific cancer imaging. Mini Rev Med Chem 2010; 10: 87-97, doi: 10.2174/ 138955710791112596.

(4.) Fani M, Maecke HR, Okarvi SM. Radiolabeled peptides: valuable tools for the detection and treatment of cancer. Theranostics 2012; 2: 481-501, doi: 10.7150/ thno.4024.

(5.) Laverman P, Sosabowski JK, Boerman OC, Oyen WJ. Radiolabelled peptides for oncological diagnosis. Eur J Nucl Med Mol Imaging. 2012; 39 (Suppl 1)S78-S92, doi: 10.1007/ s00259-011-2014-7.

(6.) De Barros ALB, Mota LG, Soares DCF, Coelho MMA, Oliveira MC, Cardoso VN. Tumor bombesin analog loaded long-circulating and pH-sensitive liposomes as tool for tumor identification. Bioorg Med Chem Lett 2011; 21: 7373-7375, doi: 10.1016/j.bmcl.2011.10.016.

(7.) Yu Z, Carlucci G, Ananias HJ, Dierckx RA, Liu S, Helfrich W, et al. Evaluation of a technetium-99m labeled bombesin homodimer for GRPR imaging in prostate cancer. Amino Acids 2013; 44: 543-553, doi: 10.1007/s00726-012-1369-9.

(8.) Accardo A, Aloj L, Aurilio M, Morelli G, Tesauro D. Receptor binding peptides for target-selective delivery of nanoparticles encapsulated drugs. Int J Nanomedicine 2014; 9: 1537-1557, doi: 10.2147/ijn.s53593.

(9.) Schroeder RP, Van Weerden WM, Bangma C, Krenning EP, De Jong M. Peptide receptor imaging of prostate cancer with radiolabelled bombesin analogues. Methods 2009; 48: 200-204, doi: 10.1016/j.ymeth.2009.04.002.

(10.) Lears KA, Ferdani R, Liang K, Zheleznyak A, Andrews R, Sherman CD, et al. In vitro. and in vivo evaluation of 64Cu-labeled SarAr-bombesin analogs in gastrin-releasing peptide receptor-expressing prostate cancer. J Nucl Med 2011; 52: 470-477, doi: 10.2967/jnumed.110. 082826.

(11.) Fani M, Maecke HR. Radiopharmaceutical development of radiolabelled peptides. Eur J Nucl Med Mol Imaging. 2012; 39 (Suppl 1): S11-S30, doi: 10.1007/s00259-011-2001-z.

(12.) Kong FL, Zhang Y, Young DP, Yu DF, Yang DJ. Development of (99m)Tc-EC-tyrosine for early detection of breast cancer tumor response to the anticancer drug melphalan. Acad Radiol 2013; 20: 41-51, doi: 10.1016/j.acra.2012.08.005.

(13.) Lambrecht FY, Durkan K, Bayrak E. Labeling bombesin-like peptide with [sup.99m]Tc via hydrazinonicotinamide: description of optimized radiolabeling conditions. J Radioanal Nucl Chem 2010; 284: 539-545, doi: 10.1007/s10967-010-0530-8.

(14.) Lambrecht FY, Durkan K, Ozgur A, Gunduz C, Avcy CB, Susluer SY. In vitro. evaluation of [sup.99m]Tc-EDDA/tricine-HYNIC-Q-Litorin in gastrin-releasing peptide receptor positive tumor cell lines. J Drug Target 2013; 21: 383-388, doi: 10.3109/1061186x.2012.757772.

(15.) Kyriazis AP, Kyriazis AA, Scarpelli DG, Fogh J, Rao MS, Lepera R. Human pancreatic adenocarcinoma line Capan-1 in tissue culture and the nude mouse: morphologic, biologic, and biochemical characteristics. Am J Pathol 1982; 106: 250-260.

(16.) Burghardt B, Wenger C, Barabas K, Racz G, Olah A, Flautner L, et al. GRP-receptor-mediated signal transduction, gene expression and DNA synthesis in the human pancreatic adenocarcinoma cell line HPAF. Peptides 2001; 22: 1119-1128, doi: 10.1016/s0196-9781(01)00433-8.

(17.) Schally AV, Szepeshazi K, Nagy A, Comaru-Schally AM, Halmos G. New approaches to therapy of cancers of the stomach, colon and pancreas based on peptide analogs. Cell Mol Life Sci 2004; 61: 1042-1068, doi: 10.1007/s00018-004-3434-3.

(18.) Szepeshazi K, Schally AV, Nagy A, Halmos G. Inhibition of growth of experimental human and hamster pancreatic cancers in vivo by a targeted cytotoxic bombesin analog. Pancreas 2005; 31: 275-282, doi: 10.1097/01. mpa.0000175892.97036.a7.

(19.) De Barros ALB, Mota LG, Ferreira CA, Oliveira MC, Goes AM, Cardoso VN. Bombesin derivative radiolabeled with technetium-99m as agent for tumor identification. Bioorg Med Chem Lett 2010; 20: 6182-6184, doi: 10.1016/j. bmcl.2010.08.124.

(20.) De Barros AL, Mota L, Ferreira CA, Cardoso VN. Kit formulation for [sup.99m]Tc-labeling of HYNIC-betaAla-[Bombesin.sub.(7-14)]. Appl Radiat Isot 2012; 70: 2440-2445, doi: 10.1016/j.apradiso.2012.06.022.

(21.) Fuscaldi LL, Barros ALB, Santos CRP, Souza CM, Cassali GD, Oliveira MC, et al. Evaluation of the optimal LNCaP prostate tumour developmental stage to be assessed by [sup.99m]Tc-HYNIC-[beta]Ala-[Bombesin.sub.(7-14)] in an experimental model. J Radioanal Nucl Ch 2014; 300: 801-807, doi: 10.1007/s10967-014-3040-2.

(22.) Faintuch BL, Teodoro R, Duatti A, Muramoto E, Faintuch S, Smith CJ. Radiolabeled bombesin analogs for prostate cancer diagnosis: preclinical studies. Nucl Med Biol 2008; 35: 401-411, doi: 10.1016/j.nucmedbio.2008.02.005.

(23.) Phillips WT. Delivery of gamma-imaging agents by liposomes. Adv Drug Deliv Rev 1999; 37: 13-32, doi: 10.1016/ s0169-409x(98)00108-2.

(24.) De Barros ALB, Mota LG, Ferreira CA, Correa NCR, Goes AM, Oliveira MC, et al. [sup.99m]Tc-labeled bombesin analog for breast cancer identification. J Radioanal Nucl Chem 2013; 295: 2083-2090, doi: 10.1007/s10967-012-2331-8.

(25.) Shi J, Jia B, Liu Z, Yang Z, Yu Z, Chen K, et al. [sup.99m]Tc-labeled [bombesin.sub.(7-14)]NH2 with favorable properties for SPECT imaging of colon cancer. Bioconjug Chem 2008; 19: 1170-1178, doi: 10.1021/bc700471z.

(26.) Larghi A, Verna EC, Lecca PG, Costamagna G. Screening for pancreatic cancer in high-risk individuals: a call for endoscopic ultrasound. Clin Cancer Res 2009; 15: 1907-1914, doi: 10.1158/1078-0432.ccr-08-1966.

F.N. Carlesso [1], L.L. Fuscaldi [1], R.S. Araujo [1], C.S. Teixeira [1], M.C. Oliveira [1], S.O.A. Fernandes [1], G.D. Cassali [2], D.C. Reis [2], A.L.B. Barros [1] and V.N. Cardoso [1]

[1] Faculdadede Farmacia, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brasil

[2] Instituto de Ciencias Biologicas, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brasil

Correspondence: V.N. Cardoso: <valbertcardoso@yahoo.com.br>.

Received November 1, 2014. Accepted February 6, 2015. First published online May 19, 2015.

Caption: Figure 1. Pancreatic adenocarcinoma experimental model at 25 days after inoculation of the human ductal Capan-1 cell line into nude male BALB/c mice. Tumor histological section images, hematoxylin and eosin staining. A, 2 x, scale bar: 500 [micro]m; B, 20 x, scale bar: 100 [micro]m: C, 40 x, scale bar: 50 mm. Arrows indicate areas of necrosis and the asterisks represent conjunctive stroma.

Caption: Figure 2. Biodistribution of [sup.99m]Tc-HYNIC-[Bombesin.sub.(7-14)] in Capan-1 tumor-bearing mice (% ID/g) after 4 h of intravenous injection of [sup.99m]Tc-HYNIC-[beta]Ala-[Bombesin.sub.(7-14)] (7.4 MBq). Data are reported as means [+ or -]SD. % ID/g: injected dose per gram of tissue.

Caption: Figure 3. Scintigraphic images (256 x 256 x 16 matrix size) obtained at 1 and 4 h after [sup.99m]Tc-HYNIC-[beta]Ala-[Bombesin.sub.(7-14)] intravenous injection (7.4 MBq) into healthy mice (left) and Capan-1 tumor-bearing mice (right) anesthetized with ketamine and xylazine. Arrows indicate tumor foci, kidneys and bladder.

Caption: Figure 4. Quantitative analysis of tumor-to-muscle (T/M) ratio evaluated by scintigraphic images. Data are reported as means [+ or -] SD. * P < 0.05, 4 h compared to 1 h (unpaired t-test).
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Author:Carlesso, F.N.; Fuscaldi, L.L.; Araujo, R.S.; Teixeira, C.S.; Oliveira, M.C.; Fernandes, S.O.A.; Cas
Publication:Brazilian Journal of Medical and Biological Research
Article Type:Report
Date:Oct 1, 2015
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