Estimation of serum calcium and magnesium in preeclampsia and eclampsia.
Preeclampsia is a progressive, multisystemic disorders characterised by triad of high blood pressure to the extent of 140/90 mmHg or more, proteinuria and with or without oedema developing after 20 weeks of pregnancy.  Eclampsia is the occurrence of convulsion in association with signs and symptoms of preeclampsia. The pathophysiological mechanism is characterised by a failure of the trophoblastic invasion of the spiral arteries, which may be associated with an increased vascular resistance to the uterine artery and a decrease perfusion of the placenta.  But the exact aetiology is still unknown, various hypothesis in the occurrence of preeclampsia have been set including the increase in thromboxane levels and the decrease in [PGI.sub.2] levels as well as an imbalance of lipid peroxidise and antioxidants. However, the results from many clinical studies show the relationship between the aggravation of the hypertension complications and the change in the concentration of various chemicals in the mother's serum.  Interestingly, significant reduction in serum calcium and magnesium are found in pre-eclamptic mothers.  Decreased serum calcium may trigger high blood pressure by stimulating renin release and parathyroid hormone secretion and also by causing vasoconstriction by its effect on vascular smooth muscle. Magnesium has been known as an essential cofactor for many enzymes and it plays an important role in peripheral vasodilatation.
Now-a-days eclampsia is considered as a multi-nutritional related disease. Attention has turned in recent years towards identifying maternal markers, which are changed in women who go on to develop preeclampsia and eclampsia which has been used in the prediction of preeclampsia and explore future areas of investigations. This study was done to estimate the serum calcium and magnesium level in preeclampsia and eclampsia with foeto-maternal outcomes.
This prospective study was carried out in the Department of Obstetrics and Gynaecology in collaboration with Department of Biochemistry of Medical College during the period from 1st July 2012 to 31st June 2013. The study was approved by Institutional Ethical Committee. Study population was divided into two groups. Group A (Control Group) consisting of 100 numbers of normotensive pregnant women after completing 20 weeks of gestation and Group B (Study Group) consisting of 50 preeclampsia and 50 eclampsia patients. They were selected randomly by lottery method. Selection of cases in Group B was done after fulfilling the inclusion criteria.
1. Pregnant woman presenting with antepartum and intrapartum eclampsia.
2. Gestational age more than 20 weeks.
3. Blood pressure [greater than or equal to] 140/90 mmHg.
4. Occurrence of convulsion with or without subsequent loss of consciousness.
5. Proteinuria [greater than or equal to] 1+ on dipstick in the random urine sample. Exclusion Criteria
1. Patients with convulsions due to causes other than eclampsia.
2. Previous history of hypertension and renal disease.
3. Postpartum eclampsia.
Blood samples were collected before magnesium sulphate was given for the treatment of Group B patient. Those patients who had received magnesium sulphate from referral centres were excluded. The estimation of magnesium and calcium was done by Calmagite and Arsenazo 3 method with normal reference value for adult was 1.8-3.0 mg/dL and 8.4-10.4 mg/dL respectively. After taking samples, treatment by magnesium sulphate was done where indicated like convulsion and imminent eclampsia. All the patients were followed up till discharge from the hospital. Data were taken in structured proforma and analysis was done using relevant statistical tools.
RESULTS AND OBSERVATIONS
There were no significant statistical differences between the study and control group regarding the age, parity, socioeconomic status, antenatal visit, gestational age and mode of delivery. In both the groups, maximum patients delivered vaginally. The commonest indication of caesarean section in both Group A and Group B was foetal distress followed by induction failure.
Table 1 shows that there is a significant decrease in the calcium and magnesium level in the preeclampsia and eclampsia group (Group B) compared to normotensives (Group A). In preeclampsia and eclampsia group also the value of calcium and magnesium are significantly lower in eclampsia (p<0.05).
Maternal complications were more in Group B (N=45) compared to normotensive group (N=15). The commonest complication in Group A was wound infection. In group B the commonest complication was post-partum haemorrhage (PPH) (53.34%) followed by wound infection (11.11%).
Foetal complications were more in Group B (N=79) compared to group A (N=17). Table 3 shows that in normotensive mothers commonest foetal complication was birth weight <2.5 kg. In group B, commonest foetal complication was weight <2.5 kg followed by preterm delivery.
Table 4 shows that the calcium and magnesium level is significantly reduced in those cases that developed complications in the preeclampsia and eclampsia group (Group B) compared to the normotensive (Group A). Two maternal deaths were found in Group B and both were eclamptic patients. Out of the 2 deaths, one was due to CVA and the other was due to pulmonary oedema.
From the present study, it was found that there is a significant decrease in the calcium and magnesium levels in the preeclampsia and eclampsia patients compared to normotensive women (p<0.0001). Also, observed is that there is a significant decrease in the levels of calcium and magnesium in those patients who developed complications in the study group compared to the control group (p<0.0001). The level of both calcium and magnesium is significantly more decreased in eclampsia than preeclampsia (p=0.0067). Similar findings were reported by Idogun ES et al  2007, in preeclampsia and eclampsia both serum calcium and magnesium levels were found to be decreased compared to normotensive women, though decrease in magnesium level was not significant. R Akhter et al  2009 reported that the level of magnesium was significantly decreased in eclamptic patients compared to normotensive women. Gupta D et al  2013 reported that there is a significant decrease in the level of serum magnesium and calcium in both preeclampsia and eclampsia compared to normal pregnant and non-pregnant women.
In the present study there were 2 maternal deaths in eclampsia group, 1 died due to CVA and the other due to pulmonary oedema. In a study done by Marina Khanum. , there were 2 maternal deaths, 1 from acute renal failure and other from CVA. Savita Rani Singhal.  reported 8 maternal deaths, 4 were due to pulmonary embolism, 2 from DIC, 1 from HELLP syndrome and 1 due to pulmonary oedema. The differences in the maternal mortalities with different studies may be due to small sample size and most of the patients who expired were excluded from the study as majority of these patients had received magnesium sulphate from the referral centres.
In the present study, 73% of the neonates were alive and healthy on discharge, but there was 9% still births and 18% neonatal deaths in preeclampsia and eclampsia group. Edgar M et al  2012 reported 79.3% neonates healthy and alive, 12.2% still birth and 8.5% neonatal deaths. Marina Khanum et al  2004 also reported 66% living neonates, 21% still births and 17% neonatal deaths, which are almost similar to the present study.
By estimation of serum calcium and magnesium level in preeclampsia and eclampsia patients in the present study, it has been found that both are significantly reduced compared to normotensive women. However, it remains unknown whether these changes are a cause or a consequence of the disease. All these observation require further confirmation by carrying out a study with a large sample size.
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Mahendra Patwari (1), Bharat Talukdar (2), Neiluotuonuo Solo (3)
(1) Professor and HOD, Department of Obstetrics and Gynaecology, FAA Medical College, Barpeta, Assam.
(3) Assistant Professor, Department of Obstetrics and Gynaecology, FAA Medical College, Barpeta, Assam.
(3) Post Graduate Trainee, Assam Medical College, Dibrugarh, Assam.
Financial or Other, Competing Interest: None.
Submission 11-06-2016, Peer Review 08-07-2016, Acceptance 13-07-2016, Published 19-07-2016.
Corresponding Author: Dr. Mahendra Patwari, Professor and HOD, Department of Obstetrics and Gynaecology, FAA Medical College, Barpeta, Assam.
Table 1: Comparison of Calcium and Magnesium Level Serum Calcium (mg/dL) Groups Mean [+ or -] SD t-value P value Group A (N=100) 9.52 [+ or -] 1.15 10.3294 <0.0001 * Group B (N=100) 8.20 [+ or -] 0.54 Preeclampsia (N=50) 8.38 [+ or -] 0.52 2.77 0.0067 * Eclampsia (N=50) 8.02 [+ or -] 0.49 Serum Magnesium (mg/dL) Groups Mean [+ or -] SD t-value P-value Group A (N=100) 2.385 [+ or -] 0.514 12.0304 <0.0001 * Group B (N=100) 1.616 [+ or -] 0.38 Preeclampsia (N=50) 1.79 [+ or -] 0.26 5.09 <0.0001 * Eclampsia (N=50) 1.44 [+ or -] 0.41 P value <0.05 considered significant, *=Significant Table 2: Maternal Complications Complications Group A (%) Group B (%) Septicaemia -- 1 (2.22%) Pulmonary oedema -- 2 (4.45%) Abruption 4 (26.67%) 4 (8.89%) Wound infection 6 (40%) 5 (11.11%) CVA -- 1 (2.22%) PPH 5 (33.33%) 24 (53.34%) HELLP Syndrome -- 1 (2.22%) Retinal detachment -- 1 (2.22%) ARF -- 2 (4.45%) Postpartum psychosis -- 1 (2.22%) Cerebral oedema -- 1 (2.22%) Neurological deficit -- 1 (2.22%) DIC -- 1 (2.22%) Total 15 (100%) 45 (100%) Table 3: Foetal Complications Complications Group A (%) Group B (%) Pre-term 3 (17.64%) 22 (27.86%) Birth asphyxia 1 (5.89%) 8 (10.11%) IUD 2 (11.76%) 9 (11.39%) IUGR 1 (5.89%) 9 (11.39%) Septicaemia -- 5 (6.33%) Weight <2.5 kg 10 (58.82%) 26 (32.92%) Total 17 (100%) 79 (100%) Table 4: Differences in the Calcium and Magnesium in those Patients who Developed Complications in Preeclampsia and Eclampsia in Comparison to Normotensive Serum Calcium (mg/dL) Groups Mean [+ or -] SD t-value P-value Patients who developed 9.52 [+ or -] 1.15 7.7168 <0.0001 * complications in Group A Patients who developed 8.14 [+ or -] 0.50 complications in Group B Serum Magnesium (mg/dL) Groups Mean [+ or -] SD t-value P-value Patients who developed 2.38 [+ or -] 0.51 9.8354 <0.0001 * complications in Group A Patients who developed 1.53 [+ or -] 0.41 complications in Group B P value <0.05 considered significant, *=Significant
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|Title Annotation:||Original Article|
|Author:||Patwari, Mahendra; Talukdar, Bharat; Solo, Neiluotuonuo|
|Publication:||Journal of Evolution of Medical and Dental Sciences|
|Date:||Jul 21, 2016|
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