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Estimation of lymphocyte apoptosis in patients with chronic non-healing diabetic foot ulcer.

Introduction

Diabetes mellitus (DM) is one of the most common non-communicable diseases affecting majority of population globally. Long-term complications of diabetes include retinopathy with potential loss of vision; nephropathy leading to renal failure; peripheral neuropathy with risk of foot ulcers, amputations, and Charcot joints; and autonomic neuropathy causing gastrointestinal, genitourinary, and cardiovascular symptoms and sexual dysfunction. [1]

The process of wound healing is very complex; it consists of haemostasis, inflammation, proliferation, and remodelling. Large number of cell types including neutrophils, macrophages, lymphocytes, keratinocytes, fibroblasts, and endothelial cells are involved in this process. [2] Lymphocytes are part of the adaptive immune response which plays a crucial role to maintain normal immune functions. These cells are carefully regulated through the equilibrium between cell birth and cell death. The participation of lymphocytes to the healing process is largely associated with their production of cytokines and growth factors (lymphokines). The role of lymphokines in wound healing has been studied in a variety of in vitro and in vivo studies. [3] Their regulatory effect on fibroblast activity and wound fibroplasia is well known. [4]

Materials and Methods

All participants involved in this study were divided into three groups. Group A included thirty healthy individuals of age group (51.33 [+ or -] 6.55 years) [mean [+ or -] SD]; group B included thirty patients with T2DM without neuropathic diabetic foot ulcer (52.50 [+ or -] 7.04 years) and group C have thirty T2DM patients with non-healing neuropathic diabetic foot ulcer (55.40 [+ or -] 7.25 years) attending SS hospital, BHU, Varanasi, India was recruited. Patients having any other complications with neuropathic diabetic foot ulcer were excluded from the study. T2DM was diagnosed according to American Diabetes Association (ADA) criteria i.e. fasting plasma glucose level ([greater than or equal to] 126 mg/dL, i.e., 7.0 mmol/L) on two occasions, or an oral glucose tolerance test (OGTT) yielding [greater than or equal to] 200 mg/dL (11.1 mmol/L) after 2 h, or symptoms of uncontrolled diabetes with a random plasma glucose level [greater than or equal to] 200 mg/dL (11.1 mmol/L). Study entrance criteria included presence of poorly controlled type-2 diabetes {glycosylated haemoglobin (HbA1C %) [greater than or equal to] 8.0}. Participants involved in this study were gone through detail clinical history and physical examination. This included age, sex, duration of diabetes, symptoms suggestive of diabetes mellitus and family history of diabetes. Physical examination included anthropometric measurements such as, height, weight, body mass index and waist circumference.

The Institutional ethical committee approved the study and written informed consent was obtained from each participant. We have followed the Helsinki guidelines, developed by the World Medical Association (WMA) on good clinical practices. Human blood samples were obtained by vein puncture in the morning while the patients were in the fasting state and kept in a heparinised tube. Peripheral blood monocytes (PBMC) were prepared by centrifugation using Ficoll-Paque[TM] plus. The number of viable cells (>95%) was determined in by Trypan blue dye exclusion test. Equal numbers of cells (1 x 106 cells/ml) were resuspended into 1X binding buffer and 100 [micro]l was taken in 5ml falcon tube for staining. Staining was performed using annexin-V-FITC kit and CD-3-PE antibody. 5 [micro]l propidium iodide (PI), 5 [micro]l annexin-V-FITC and 10 [micro]l CD-3-PE were added and the cells incubated for 15 min in the dark, after which 400 [micro]l binding buffer was then added to each tube and the cells analyzed by flow cytometry within 30 min. Flow analysis was performed on a FACScan (Becton Dickinson, CA) with a 488-nm argon laser. A quantity of 10,000 cells was collected and analyzed.

Total lymphocytes undergoing early and late apoptosis in T2DM patients with and without ulcer were compared with those of the control subjects. Statistical analysis was performed within these three groups by One-way analysis of variance (ANOVA). Newman-Keuls post-test was used for multiple comparisons. The differences were considered statistically significant when p <0.05.

Results

Significance difference was observed in FBG, PPBG and HbA1c whereas there was no significance difference in the age and body mass index (BMI) in between the all three groups. The percentage of CD-3 + annexin-V-FITC-positive cells was 14.76 [+ or -] 4.61% in group A, 22.52 [+ or -] 8.47% in group B, and 28.36 [+ or -] 15.41% in group C. The percentage of CD-3 + PI-positive cells was 0.44 [+ or -] 0.38 in group A, 0.90 [+ or -] 0.53 in group B, and 1.17 [+ or -] 0.26 in group C. CD-3 + Annexin-V-FITC positive lymphocytes were statistically significant in group C (P<0.01) and group B (p<0.001) when compared from group A. The value of significance was p<0.05 when compared between group B and group C. CD-3 + PI positive lymphocytes were highly significant in group C and group B when compared from group A (p<0.001). The value of significance was p<0.05 when compared between group B and group C (Figure 1 A & B).

Discussion

Lymphocytes provide cell mediated immunity and are capable of producing growth factors to take part in the setting of wound repair. [5] T cell-dependent immune system plays an active role in the process of wound healing; its depletion leads to impaired wound healing. [6] Several studies suggest that wound healing is delayed due to decreased T-cell infiltration and concentration in the wound site where as others have reported that CD 4+ cells have a positive role and CD8+ cells play an inhibitory role in wound healing. [7] Wound healing impaired by radiation reflects reduced expression of lymphocyte subtypes. [8] The role of T lymphocyte in diabetic foot ulcer is not completely understood and is a current area of intensive investigation. In this report, we demonstrated that the total T-cell apoptosis is significantly higher in T2DM with diabetic foot ulcer as compared with T2DM without ulcer and healthy control. Our previous studies show that increased oxidative stress in diabetics with chronic non healing wound induces lymphocyte apoptosis. [9] Lymphocytes regulated the formation of fibrous tissue at wound site and alteration in host immune response alters the course of fibroplasia after wounding. [10] T lymphocytes play a crucial role in the wound healing process and its removal inhibits the healing cascade.

Conclusion

Our study provides basic information regarding increased T lymphocyte apoptosis in diabetic foot patients and shows that increased T lymphocyte apoptosis in diabetic foot patients is responsible for delayed wound healing with other factors. A detailed and thorough research is required to understand the mechanism by which T cells up regulate wound healing in diabetic foot patients.

ACKNOWLEDGMENTS

Author Mr. AK Arya is thankful to CSIR, India, for financial support.

References

[1.] American Diabetes Association. Diagnosis and Classification of Diabetes Mellitus. Diabetes care. 2010;33:S62-69.

[2.] Guo S, DiPietro LA. Factors Affecting Wound Healing. J Dent Res. 2010; 89(3): 219-229.

[3.] Schaffer M, Barbul A. Lymphocyte function in wound healing and following injury. Br J Surg. 1998; 85:444-460.

[4.] Wahl SM, Allen JB. T lymphocyte-dependent mechanisms of fibrosis. Prog Clin Biol Res. 1988; 266:147-60.

[5.] Blotnik S, Peoples GE, Freeman MR, Eberlein TJ, Klagsbrun M. T lymphocytes synthesize and export heparin-binding epidermal growth factor-like growth factor and basic fibroblast growth factor, mitogens for vascular cells and fibroblasts: differential production and release by CD41 and CD81 T cells. Proc Natl Acad Sci. 1994; 91: 2890-94.

[6.] Swift ME, Burns AL, Gray KL, DiPietro LA. Age-related alterations in the inflammatory response to dermal injury. J Invest Dermatol. 2001;117:1027-35.

[7.] Schaffer M, Stulten C, Viebahn R. Role of T-lymphocytes in radiation-impaired wound healing. Pol Przegl Chir. 2007;79(4):312-18.

[8.] Efron JE, Frankel HL, Lazarou SA, Wasserkrug HL, Barbul A. Wound Healing and T-Lymphocytes. J Surg Res. 1990;48:460-63.

[9.] Arya AK, Pokharia D, Tripathi K. Relationship between oxidative stress and apoptotic markers in lymphocytes of diabetics with chronic non healing wound. Diabetes Res Clin Pract. 2011, 94(3):377-384.

[10.] Neilson EG, Phillips SM, Jimenez S. Lymphokine moduation of fibroblast proliferation. J Immunol. 1982; 128:1484-86.

Source of Support: Council of Scientific & Industrial Research (CSIR), India

Conflict of interest: None declared

DOI: 10.5455/ijmsph.2013.100620132

Received Date: 13.03.2013

Accepted Date: 10.06.2013

Awadhesh Kumar Arya, Sunny Garg, Santosh Kumar, Lalit P Meena, Kamlakar Tripathi

Institute of Medical Sciences, Banaras Hindu University (BHU), Varanasi, Uttar Pradesh, India

Correspondence to: Awadhesh Kumar Arya (awi.imsbhu07@gmail.com)

Table-1: Baseline Characteristics of the Study
Participants

Parameter              Groups (Mean [+ or -] SD)

                A                 B                 C

Number          30                30                30
Age (Years)      51.33 [+ or -]    52.50 [+ or -]    55.40 [+ or -]
                  6.55              7.04              7.25
Duration of     --                 30.42 [+ or -]    59.57 [+ or -]
  DM (Months)                      18.32              9.51
BMI (kg/         23.57 [+ or -]    23.91 [+ or -]    24.88 [+ or -]
  [m.sup.2])      3.60              1.66              2.09
FBS (mg/dl)      93.67 [+ or -]   152.03 [+ or -]   170.63 [+ or -]
                  5.62             13.59             16.41
PPBS (mg/dl)    128.66 [+ or -]   232.16 [+ or -]   252.03 [+ or -]
                  5.56             20.66             19.55
HbA1c (%)         5.93 [+ or -]     8.81 [+ or -]    10.06 [+ or -]
                  0.34              0.81              1.99

Parameter               p value

                A vs B & C   B vs C

Number
Age (Years)     NS           NS

Duration of                  <0.001
  DM (Months)
BMI (kg/        NS           NS
  [m.sup.2])
FBS (mg/dl)     <0.001       <0.001

PPBS (mg/dl)    <0.001       <0.001

HbA1c (%)       <0.001       <0.001
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Article Details
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Title Annotation:RESEARCH ARTICLE
Author:Arya, Awadhesh Kumar; Garg, Sunny; Kumar, Santosh; Meena, Lalit P.; Tripathi, Kamlakar
Publication:International Journal of Medical Science and Public Health
Article Type:Report
Geographic Code:9INDI
Date:Oct 1, 2013
Words:1586
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