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Epizootiology of columnaris disease in aquaculture and sport fishing ponds located within a bounded hydrologic system.

EPIZOOTIOLOGY OF COLUMNARIS DISEASE IN AQUACULTURE AND SPORT FISHING PONDS LOCATED WITHIN A BOUNDED HYDROLOGIC SYSTEM HAITHAM H. MOHAMMED AND COVADONGA R. ARIAS, SCHOOL OF FISHERIES, AQUACULTURE, AND AQUATIC SCIENCES, AUBURN UNIVERSITY, AUBURN, AL 36849.

Flavobacterium columnare, the etiologic agent of columnaris disease, is one of the most significant bacterial pathogens of freshwater wild and cultured fishes both in the US and worldwide. However, the epizootiology of columnaris disease in fish ponds has not been extensively studied. Recently, we have observed abnormally rigorous outbreaks of columnaris at the E.W. Shell Fisheries Center, Auburn, AL. Throughout these outbreaks, not only catfishes were affected but also other aquaculture and sport fish species that were in ponds supplied by the same watershed. The purpose of this study was to examine the genetic diversity among F. columnare isolates collected from different sample sources, sites and dates in an attempt to clarify the origin of these outbreaks. From spring 2010 till summer 2012, 102 F. columnare isolates were recovered from catfishes, bluegill, tilapia, largemouth bass, catfish egg masses and water samples. Putative colonies were identified by their unique morphology on agar plates and confirmed by specific PCR amplification. Genomovar ascription by RFLP analysis of the 16S rRNA gene grouped all the isolates under genomovar II. Genetic variability among the isolates was further studied by AFLP fingerprinting. Antibiogram was used to test the susceptibility of the isolates to the most common antibiotics. Our results showed distribution of specific F. columnare types throughout the watershed over the study period and resistance of some isolates to oxytetracycline and kanamycin.

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Title Annotation:Biological Sciences Paper Abstracts
Publication:Journal of the Alabama Academy of Science
Article Type:Author abstract
Date:Apr 1, 2014
Words:262
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