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El ciclo de vida de una arana desertica deducido a partir de la distribucion de frecuencia de tallas.

The life cycle of a desert spider inferred from observed size frequency distribution

INTRODUCTION

The life history of any species is related to the species distribution, habitat use and energetic requirements, the biological relationships with other species and the structure and dynamics of communities (Polis et al. 1996; Begon et al. 2006). There are many studies of post-embryonic development and the identification of spiders instars by direct observation of captive specimens (Aguilar & Mendez 1971; Jackson 1978; Bartos 2005; Punzo & Farmer 2006), but this takes a lot of time and effort (Toft 1976). An alternative method is to deduce the life cycle of a species based on the analysis of different body size measurements of organisms systematically collected during one or two years (Toft 1976; Aitchinson 1984; Aiken & Coyle 2000; Wright & Coyle 2000; Crews et al. 2008). Most of these studies identified spider size classes by using frequency histograms, assuming that most conspicuous modes represent size groups while recognizing that the boundaries among them are arbitrary (Toft 1976; Davis & Coyle 2001). However these histograms present several problems, including dependence of grid origin and the interval width, discontinuity and the use of fixed width intervals. These problems have motivated statisticians to find alternative, more efficient methods. Kernel Density Estimators (KDEs) do not depend on the origin position and are continuous distribution estimators (Silverman 1986). Besides, there are several methods for choosing the interval width (Hardle 1991; Scott 1992). Most of these methods have been employed to describe fish size classes and growth (Sanvicente-Anorve et al. 2003); however they have not been used to describe spider size classes. Here we present the use of KDEs as a modern tool to examine body sizefrequency distributions of spiders, using Syspira tigrina Simon, 1885 as an example.

The species Syspira tigrina Simon, 1885 (Araneae: Miturgidae) is very abundant in xeric areas from North America. This species is one of the most conspicuous spiders in Baja California Sur, representing up 40% of all wandering spiders (NietoCastaneda & Jimenez-Jimenez 2009; Jimenez & Navarrete 2010). This miturgid is a fast-moving ground-hunting spider and its body size range from 5 to 18 mm; males are same or bigger size than females (Ubick & Richman 2005; Nieto-Castaneda & Jimenez-Jimenez 2009). In turn, this species is an important prey diet component for some rodents (Alvarez-Castaneda et al. 2006), and possibly be preyed upon by other vertebrates and invertebrates due to its high abundance. Nevertheless, information on its biology is scarce.

METHODS

Study area. We worked in El Comitan, a federal biological preserve station located near Bahia de La Paz (24[grados]7'N, 110[grados]25'W, 20 m a.s.l.), west of La Paz city in Baja California Sur, Mexico. The region is a subtropical desert with hot summers, a sporadic rainy season between July and October, and warm winters with little or no rain between November and February. The vegetation is a subtropical desert shrub (Leon de la Luz et al. 1996).

Field work. We collected spiders every month from July 2005 to July 2006 by establishing two 150 m line transects, each one with 15 pitfall traps at approximately 10 m intervals (Muma 1981). A wire covering on each trap excluded dead leaves and other debris but not spiders. The samples were preserved in 70% ethanol and transferred to the laboratory for cleaning and taxonomic identification.

Species identification and measurements. We grouped the spiders as juveniles, penultimate adults (males and females) and adults (males and females). Then we measured the tibia I length (TIL) (distance along the dorsal surface from proximal joint to distal joint with the leg on the horizontal plane) as an estimator of body size (Toft 1976), using a graduated lens under the stereoscopic microscope with each specimen submerged in 70% ethanol and anchored in a bed of fine white sand on the bottom of a culture dish. Voucher specimens were deposited in the Arachnological and Entomological Collection of the Centro de Investigaciones Biologicas del Noroeste (CAECIB).

Data analysis. We analyzed the TIL distribution of each monthly sample by means of Kernel Density Estimators (KDEs), using weighted averaging of rounded points (WARP) (Hardle & Scott 1992; Salgado-Ugarte et al. 1995a). KDEs are defined as:

[MATHEMATICAL EXPRESSION NOT REPRODUCIBLE IN ASCII]

Where,

f = density estimation of the variable x

n = number of observations

h = bandwidth

[X.sub.i] = tibia length of the i-th spider specimen

K(*) = a smooth, symmetric kernel function integrating to one.

In this case, we use the Gaussian kernel function, i.e.:

K (z) = 1/[square root of (2 [pi])] exp (-[z.sup.2]/2)

where

z = (x-[X.sup.i]/h

The bandwidth h was chosen based on Silverman's rule (1986) (Salgado-Ugarte et al. 1995b):

h = [0.9 A/[n.sup.1/5]] p

where

[MATHEMATICAL EXPRESSION NOT REPRODUCIBLE IN ASCII]

A is the smaller of two estimates of the standard deviation: the usual estimate S or F-pseudosigma, a robust alternative for S (Hoaglin 1983). The Fourth-spread is a resistant dispersion measure approximately equivalent to the interquartile range (Tukey 1977). The Silverman optimal bandwidth is designed for Gaussian distributed data. This value is too large when applied with skewed or multimodal distributions. Nevertheless, in these cases the optimal bandwidth may serve as a starting point which allows the dominant modes to be recovered.

The optimal bandwidth chosen for each TIL size group reflects reference values calculated with several practical rules for oversmoothed and optimal widths (Salgado-Ugarte et al. 1995b) and the values determined by the smoothed Bootstrap multimodality test of Silverman (1986) (Salgado-Ugarte et al. 1997). The resulting density distributions were scaled to a smoothed frequency and decomposed into their Gaussian components with a computerized version of the method proposed by Bhattacharya (1967) (Salgado-Ugarte et al. 1994). Since the slope of a Gaussian curve is positive to the left of the mode, zero at the mode and negative to the right of the mode, the logarithmic derivative decreases linearly. Therefore, each Gaussian component can be determined by identifying negative sloped intervals in the graph of logarithmic frequency differences against length midpoints (Salgado-Ugarte et al. 1994). Then each Gaussian component represents a TIL size group. We analyze the TIL groups within samples by plotting monthly TIL frequency histograms with over imposed monthly KDEs for all TIL groups. The KDEs result in figures smoother than histograms, allowing easy recognition of characteristics such as outliers, skewness, and multimodality (Salgado-Ugarte et al. 1993, 1995b). All statistical procedures were performed in Stata v. 9.1 (StataCorp 2001).

RESULTS

We collected 914 spiders, 660 were juveniles, 69 penultimate adults and 185 adults of S. tigrina. We found that adult spiders were more abundant between August and November 2005 and penultimate adults were more abundant between July and August 2005; both had low abundance or were absent the remainder of the year (Fig. 1) while juveniles were present all year.

[FIGURE 1 OMITTED]

Because use KDEs analysis is to delimit size groups continuously we only analyzed juvenile TIL distribution (Fig. 2), and excluded the other spiders because we already identified them as penultimate adults and adults.

The TIL-frequency distributions of juveniles indicate that most part of the year small sizes (TIL mean < 1 mm) had high frequency. TIL mean varied from 0.9752 to 1.0518 mm. Optimal TIL bandwidths used to construct KDEs varied between 0.053 to 0.3425 mm depending on the number of observations and their variation. In most cases we observe a dominant mode with several minor modes. Assuming Gaussian distribution, we estimated the statistics: mean, standard deviation and size (Table 1) corresponding to each main mode by the Bhattacharya's method (Fig. 3). Dominant TIL modes indicate spider groups.

We found 35 spider groups (Gaussian components) with TIL frequency distributions during the 12 month collection period (Table 1). The smallest TIL groups were more abundant between October 2005 and January 2006 (N = 284, TIL mean < 0.65 mm) and the biggest were more abundant twice, one from May to July 2006 (N = 196, TIL mean > 0.75 and < 1.92) and from July to August 2005 (N = 101, TIL mean > 0.75 and < 1.63).

DISCUSSION

The abundance patterns of adult spiders of S. tigrina indicate that the mating period might occur between August and October 2005 because it is when most adults were present. The high abundance of penultimate males and females previous to August 2005 confirms this outcome. This period takes place just after the main rainy season and when temperature starts to drop (Fig. 4). This it is compatible with the breeding season of most desert animals (Keast & Marshall 1954; Barrientos et al. 2007).

Also the juvenile TIL groups also indicate that the principal recruitment period might occur during November and December and, to a lesser extent, on January when the smallest juveniles (TIL mean < = 0.7 mm) were more abundant, and also when larger specimens (TIL mean < = 0.7 mm) were scarcer in the study period. This recruitment occurs after the main rainy season and when the temperature starts to decrease (Fig. 4).

The pattern described above suggests that there should be a continuous recruitment through the year (the highest values occurs between November and December 2005), implying that the females oviposit almost throughout the year, starting in November and gradually less frequently afterwards. There is evidence of juvenile growth from January to February, when the dominant group classes were > 0.7 mm of TIL mean, but growth continued until May- July. This suggests that the maturation and mating periods are close to each other. The patterns we found through this analysis are similar to those observed earlier for the desert spider Diguetia mojavea, where reproduction and oviposition occurs during July and October, and no juveniles are found during August to December (Boulton & Polis 1999). The continuous oviposition periods of the females could be a consequence of the characteristic desert harsh conditions (Polis & Yamashita 1991), as reported for the desert spider Clubiona robusta, which oviposits several times in at least six months in one year (the recruitment period of the species cannot be determined because their juveniles are impossible to identify from other clubionids) (Austin 1984). Unfortunately there are few comparable life history studies of other desert spiders. The lower abundance of the larger juveniles (TIL mean > 0.7 mm) during most part of the year suggests that they must be dying. For this or any other reason they cannot be caught by the pitfall traps.

[FIGURE 2 OMITTED]

[FIGURE 3 OMITTED]

[FIGURE 4 OMITTED]

In general, there are few studies about the life histories of ground dwelling spiders around the world. This might be due to the difficulty of differentiating juveniles among related species, and discriminating size classes in mixed length frequency distributions (Toft 1976). However, it is interesting that in Holarctic regions some spider species from several families (Araneidae, Clubionidae, Linyphidae, Theridiidae, Lycosidae, mainly), mate and ovipositate during May and October (Toft 1976; Aiken & Coyle 2000; Wright & Coyle 2000; Davis & Coyle 2001); these species have well defined life histories where the mature adults occur once or twice in a year, however in all cases the limits of size classes are unambiguous.

The use of KDEs is a powerful statistical tool to delimitate size groups with mixed frequency distributions which otherwise would be difficult to determine, not only for life cycle studies. Animal group size is one of the most important characteristics that structures populations and communities, then it must be necessary to test any biological hypothesis based on size groups.

Acknowledgements. We thank F. A. Coyle for his suggestions; M. M. Correa-Ramirez assisted during field collections and L. Higgins for her comments and valuable editorial improvements and two anonymous reviewers for the time and concern spent on the review of this manuscript. This project was supported by Consejo Nacional de Ciencia y Tecnologia of Mexico (SEMARNAT-CONACYT C01-0052). Additional support was provided by UNAM (PAPIME PE205407). Collection, study, and preservation of specimens comply with Mexican law, including a permit from SEMARNAT.

Recibido: 19/08/2011; aceptado: 21/03/2012.

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IRMA GISELA NIETO-CASTANEDA, (1) ISAIAS HAZARMABETH SALGADO-UGARTE (2) & MARIA LUISA JIMENEZ-JIMENEZ (3)

(1) Universidad del Mar, Campus Puerto Escondido (UMAR), Ciudad Universitaria, Carretera Via Sola de Vega, Puerto Escondido, San Pedro Mixtepec, Juquila, Oax., Mexico C.P. 71980 Mexico; Tel: +(52) (954) 954 58 249 90. <irmanieto@zicatela.umar.mx> (2) Laboratorio de Biometria. Facultad de Estudios Superiores Zaragoza, Universidad Nacional Autonoma de Mexico (FESZ, UNAM), Batalla 5 de mayo S/N esq. Fuerte de Loreto, Ejercito de Oriente, Iztapalapa 09230, Mexico, D.F., Mexico. <isalgado@servidor.unam.mx> (3) Centro de Investigaciones Biologicas del Noroeste Mar Bermejo 195, Col. Playa Palo de Sta. Rita La Paz, B.C.S., 23090 Mexico Tels: (612) 12 3 8481. <ljimenez04@cibnor.mx>
Table 1. Juvenile TIL groups (Gaussian components)
found with their parameters of twelve samples
of Syspira tigrina collected at El Comitan.

Date               Gaussian    Component
                   component     range

26 July 2005           1         11-23
                       2         21-27
                       3         40-43
27 August 2005         1          8-14
                       2         20-27
4 October 2005         1          5-14
                       2         17-21
                       3         31-35
                       4         38-42
6 November 2005        1         10-17
                       2         27-33
9 December 2005        1          5-17
                       2         22-26
                       3         36-42
                       4         47-59
16 January 2006        1          8-16
                       2         23-25
                       3         30-37
14 February 2006       1          3-15
                       2         23-32
                       3         44-56
                       4         62-69
11 March 2006          1          3-19
19 April 2006          1         11-20
                       2         32-38
                       3         43-52
24 May 2006            1         10-15
                       2         19-24
                       3         35-40
24 June 2006           1          7-13
                       2         21-25
                       3         30-53
                       4         38-42
22 July 2006           1         11-14
                       2         25-30

Date                 Mean      Standard     Component
                               deviation     size

26 July 2005        0.7710       0.2530       22
                    1.6270       0.4041       58
                    2.2967       0.1752       1
27 August 2005      0.5874       0.2862       31
                    1.5863       0.4425       20
4 October 2005      0.6051       0.1618       36
                    0.9644       0.1787       15
                    1.5241       0.3452       10
                    1.9755       0.1846       2
6 November 2005     0.6228       0.2343       78
                    1.8053       0.2804       9
9 December 2005     0.5845       0.1022       69
                    0.8429       0.1268       21
                    1.3235       0.1031       2
                    1.8242       0.0924       2
16 January 2006     0.6410       0.3014       36
                    1.6785       0.3992       4
                    2.2803       0.2514       1
14 February 2006    0.6606       0.1153       21
                    0.9731       0.1030       9
                    2.1546       0.0986       2
                    2.3748       0.0930       2
11 March 2006       0.5712       1.0513       25
19 April 2006       0.7964       0.1764       35
                    1.3708       0.1629       14
                    1.7374       0.1073       2
24 May 2006         0.8676       0.2887       21
                    1.4966       0.2492       14
                    2.4496       0.2166       2
24 June 2006        0.6410       0.1502       9
                    1.3036       0.2583       37
                    1.7725       0.1846       13
                    2.1511       0.1585       5
22 July 2006        0.7590       0.3365       23
                    1.9140       0.6224       15
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Author:Nieto-Castaneda, Irma Gisela; Salgado-Ugarte, Isaias Hazarmabeth; Jimenez-Jimenez, Maria Luisa
Publication:Acta Zoologica Mexicana (nueva serie)
Date:May 1, 2012
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