Printer Friendly

Effects of xylanase on performance, blood parameters, intestinal morphology, microflora and digestive enzyme activities of broilers fed wheat-based diets.


Although wheat is becoming an important source of energy in poultry diets, its high level of xylans, the principal water-soluble non-starch polysaccharides (NSP), limits its use. The presence of xylans increases the viscosity of the digesta, impeding the digestion and absorption of nutrients and causing poor performance (Almirall et al., 1995; Choct et al., 1995). There is considerable evidence that negative effects of NSP in poultry diets are related to the gut microflora of broilers, as supplementation of antibiotics to diets increases their nutritive value (Annison and Choct, 1991). Diet composition may produce microscopic alterations in the intestinal mucosa (Yamauchi, 2002) and it is possible that the change in morphology of the gastrointestinal tract (GIT) may be associated with dietary NSP levels. NSP also alters digestive functions and, in particular, digestive enzyme activities. It has been demonstrated in rats that there may be an attempt to compensate for the inefficiency of digestion and absorption with hyperplasia and hypertrophy of digestive organs and an increased secretion of digestive juice, although nutrient digestibility does not improve (Ikegami et al., 1990).

Adding NSP-degrading enzymes is a routine practice to improve the performance of broilers fed diets based on rye, wheat, barley or oats (Bedford, 2000a; Acamovic, 2001; Cowieson, 2005). However, although the efficacy of exogenous enzymes has been well established, the underlying mechanism is not clearly understood. It has been proposed that the NSP-degrading enzymes reduce digesta viscosity in the small intestine, and result in improvements in nutrient absorption. In vitro study showed that the endosperm cell wall of barley was completely degraded by the NSP-degrading enzyme and supplementation with NSP-degrading enzymes increased the digestibilities of dry matter, crude protein, nitrogen-free extract, crude fat and crude fiber of barley by 18.1%, 20.3%, 16.4%, 26.9% and 30.0%, respectively (Li et al., 2004). Some studies have demonstrated that enzyme treatment can influence the intestinal morphology of birds fed barley-based diets (Brenes et al., 1993) and there are interactions between enzymes and the host animal, its microflora, and also dietary ingredients (Bedford, 2002).

Furthermore, the growth performance of poultry is also closely associated with the regulation of metabolism and function of the growth-related endocrine system. There is a high correlation between the relative growth rate of broiler cockerels and the concentrations of some hormones such as IGF-I and tri-iodothyronine ([T.sub.3]) (Buys et al., 1999). Nutritional status is an important factor in the regulation of blood hormones and intermediary metabolism in broiler chickens (Buyse et al., 2002; Swennen et al., 2005). However, relatively few studies have been conducted to evaluate the effects of different levels of xylanase on intestinal development and hormone levels of broilers fed wheat-based diets. Besides, some researchers showed that excess supplementation of enzyme complex had no effect on performance or even inhibited endogenous enzyme secretion and destroyed small intestine structure (Iji et al., 2001; Ai et al., 2004). However, few reports are available on the effects of a high level of xylanase as a single source of enzyme on the performance and intestinal parameters of broilers. Therefore, the objective of the present study was to investigate the effects of different levels of xylanase on performance, blood hormones, intestinal morphology, microflora and digestive enzyme activities in broilers fed wheat-based diets.


Enzyme preparation

The enzyme preparation used in this study was a microbial xylanase containing 10,000 U/g xylanase activity. One unit of xylanase is defined as the amount of enzyme that liberates 1 ^mol of reducing sugars from 5 mg/ml at xylan solution per min at pH 5.5 and 37[degrees]C.

Birds and diets

A total of 240 one-day-old Arbor Acres, female broiler chicks were obtained from a commercial hatchery. The chicks were weighed and allocated to 4 dietary treatments in a completely randomized design. Each treatment was replicated 5 times with 12 chicks each. The birds had free access to feed and water. The experimental period was divided into 2 phases: growing phase (1 to 21 d) and finishing phase (22 to 42 d). The compositions of the diets are listed in Table 1. The 4 dietary treatments were wheat-based diets adequate in all nutrients for both experimental phases and supplemented with 0, 500, 1,000, and 5,000 U/kg xylanase. Diets of the finishing phase were antibiotic-free. All experimental diets were given in mash form. Diets were formulated to the nutrient requirements recommended by NRC (1994) for broilers of each matching age.

All chicks were provided 24-h light for the first three days, followed by 18 h of light and 6 h of dark for the growing phase and 16 h of light and 8 h of dark for the finishing phase. Room temperature was maintained at 33 [degrees]C for the first three days and then gradually reduced 2 to 3[degrees]C per week to a final temperature of 22[degrees]C. House conditions and animal management followed standard recommendations (Institute of Laboratory Animal Resources Commission on Life Science, 1996).

On d 21 and d 42, birds were weighed by the pen. Feed consumption was recorded weekly and mortality was recorded daily.

Sample collection

On d 42, one bird from each pen with body weight closest to the mean was selected and killed by cervical dislocation. The digesta from the duodenum, jejunum and ileum were added into ice-cold deionized water. The mixture was homogenized and centrifuged (13,000 xg for 5 min) and the supernatant was transferred into a 2 ml eppendorf tube immediately and frozen at -20[degrees]C until analyzed.

On d 21 and d 42, one bird from each pen was selected randomly, and blood was obtained after morning feeding by heart puncture for the determination of blood glucose, uric acid, insulin and IGF-I. Blood samples were allowed to clot at 4[degrees]C and centrifuged at 1,520 xg for 20 min before harvesting serum. Serum samples were stored at -20[degrees]C until assayed. For broilers at 21 d, approximately 5 cm lengths of the duodenum, jejunum and ileum were removed for measurements of intestine histology.

Bacteriological analysis

On 42 d, five broilers from each treatment were randomly selected and killed by cervical dislocation. The following procedures were conducted according to the method described by Zhang et al. (2003). About 1 g of ileal or cecal contents was placed into a bottle containing 50 ml of sterilized physiological salt solution (NaCl, 9 g/L) together with a few glass beads to aid dispersion. The sample was homogenized and the suspension was then serially diluted to [10.sup.-8] in 9 ml of sterilized physiological salt solution for viable counts of total aerobes, Lactobacillus, and E. coli. Aliquot volumes (0.2 ml) of appropriate dilutions were spread on the appropriate selective agar plates and incubated at 37[degrees]C. Nutrient agar was used as the medium for counting total aerobes with dilutions of 10-2 to [10.sup.-6]. Aliquots of these dilutions were also placed onto MacConkey agar for E. coli counts. Nutrient agar and MacConkey agar were incubated aerobically for 1 d. For Lactobacillus, Rogasa medium was used with dilutions of [10.sup.-4] to [10.sup.-8], and the plates were incubated in 5% C[O.sub.2] for 48 h. All dilutions were plated in duplicate. After incubation, colonies were counted according to their morphology. Counts from duplicate plates were averaged. Numbers of colony-forming units were expressed as log colony-forming units per gram of the digesta content.

Intestinal morphology

Examinations of intestinal morphology were carried out according to the method of Iji et al. (2001). Intestine samples from each section were fixed in 10% buffered formalin until analyzed. Each segment was embedded in paraffin. A section of each sample was placed onto a glass slide and stained with alcian blue/haematoxylin and eosin for examination with a light microscope. Villus height, crypt depth, and the thickness of epithelium and muscle were measured at 100xmagnification using computer software (Sigma Scan, Jandel Scientific, San Rafael, CA. USA), and then the ratio of villus height to crypt depth and villus surface area were calculated.

Chemical analysis

The activities of amylase and protease of the small intestinal digesta were determined using standard kits (Jiancheng Bioengineerign Institute, Nanjing, China). The concentrations of blood glucose and uric acid were measured by kits (Shanghai Fuxing Changzheng Medical Science, Ltd. Co., Shanghai, China), and insulin and IGF-I were measured by RIA using standard kits (Tianjin Jiuding Biological Technology Ltd. Co., Tianjin, China). All blood parameters were measured according to the manufacturer's instructions. All measurements for each variable were run in the same assay in order to avoid inter-assay variability.

Statistical analysis

Data were analyzed using the General Linear Model procedure of SAS (SAS Institute, 1996) to determine the treatment effects. Means with a significant F ratio were separated by the least significant difference test. Differences were considered significant at p<0.05.


Birds were in good health throughout the experimental period. Mortality was less than 0.2% and was not related to dietary treatment. At the start of the experimental period, there were no differences (p>0.05) in initial BW among the treatments.

The effects of exogenous xylanase supplementation on the performance of broilers fed wheat-corn-based diets are shown in Table 2. Different levels of xylanase tended to increase (p>0.05) body weight gain (BWG) during the finishing phase. Enzyme had no significant effects on average daily feed intake (ADFI) of broilers in both phases. Feed:gain ratio was significantly improved by enzyme supplementation in broilers from 1-21 d and 1-42 d (p<0.05). There were no significant differences in the performance of broilers among levels of enzyme supplementation in both phases.

Xylanase supplementation had no influence on intestinal microflora of broilers at 42 d (Table 3). However, supplementing 500 U/kg and 1,000 U/kg xylanase tended to reduce (p = 0.06) the counts of E. coli in the ileum compared with the control.

Supplementing 500 U/kg and 1,000 U/kg xylanase increased (p<0.05) the villus height in the duodenum, jejunum and ileum (Table 4). There was no significant difference between the two levels except in the jejunum where 1,000 U/kg increased the villus height (p<0.01). In the duodenum and jejunum, there was no significant difference in villus height between 5,000 U/kg xylanase and the control, but the height was decreased in the 5,000 U/kg group compared with 500 U/kg and 1,000 U/kg groups (p<0.05). In the ileum, 5,000 U/kg increased the villus height compared with the control (p<0.01). The ratio of villus height to crypt depth was increased by 1,000 U/kg xylanase supplementation in the duodenum, jejunum and ileum (p<0.01). There was a significant difference in the ratio between 500 U/kg xylanase supplementation and the control (p<0.05) in the ileum. 5,000 U/kg treatment increased the height in the duodenum and ileum compared with the control (p<0.05). However, xylanase supplementation had no effects on crypt depth, epithelial thickness and intestinal muscle thickness in the three segments.

No significant differences were observed among dietary treatments on amylase and protease activities of the small intestinal digesta (Table 5) and on blood parameters (Table 6).


It is well documented that supplementing exogenous enzymes to wheat-based diets for broilers can improve performance (Peng, 2003; Wang et al., 2005). The xylanase preparation improved weight gain and feed:gain ratio throughout the experiment. The feed to gain ratio was decreased by 9.11%, 8.11%, 7.88% during 1-21 d and 9.96%, 7.50%, 11.27% during 1-42 d with 500 U/kg, 1,000 U/kg, and 5,000 U/kg xylanase supplementation, respectively. However, the effects on feed to gain ratio were not significant among the three different levels of xylanase supplementation (Table 2). The data were in general agreement with those of Wang et al. (2005) and Gao et al. (2008). The improved performance may be due to lowered viscosity and/or disruption of cell wall. However, the feed:gain ratio over 22-42 d was not improved by xylanase addition. This may be explained by effects of enzyme supplementation being dependent on the bird's age and older birds having a greater capacity to endure the effects of high viscosity because of enhanced fermentation capacity of the microflora in their intestines (Vukic-Vranjes and Wenk, 1995; Choct et al., 1996).

Composition of the diet affects the gastrointestinal microflora in broilers. The presence of viscous polysaccharides has been shown to increase the intestinal microbial activity associated with poor broiler performance (Choct et al., 1996; Hubner et al., 2002). Enzyme supplementation can significantly influence microbial populations in the intestine. NSP-degrading enzymes such as xylanase are hypothesized to work in two steps, described as an ileal phase and a cecal phase (Bedford, 2000b). During the ileal phase, enzymes remove fermentable substrates. During the cecal phase, degradation products of sugars, such as xylose and xylo-oligomers, are fermented by cecal bacteria, thus stimulating the production of VFA and the growth of specific beneficial bacteria (Bedford, 2000b). Engberg et al. (2004) found that xylanase addition to wheat-based broiler diets stimulated growth of lactic acid bacteria in the ileum, which was confirmed by higher lactic acid concentrations. However, in the present study, there was no influence of xylanase on the counts of Lactobacillus, E. coli and total aerobes in the ileum and caecum (Table 3). These results are consistent with those of Gao et al. (2008) who reported no significant change of lactobacillus and coliform bacteria counts in caecum contents of 21-day-old birds. In our study, we observed that in the ileum there was a tendency for decreased (p = 0.06) counts of E. coli when 500 U/kg and 1,000 U/kg xylanase were added. The ceca contain the largest number of bacteria in the chicken GIT so the regulation of microflora composition by diet ingredients might be more complicated than in the ileum.

In the present study, compared with the control 500 U/kg and 1,000 U/kg xylanase supplementation increased villus height of the duodenum, jejunum and ileum. The ratio of villus height to crypt depth of the three segments was increased when 1,000 U/kg xylanase was added but only the ratio in the ileum was increased by 500 U/kg xylanase (Table 4). A significant positive correlation between xylan level in wheat and the relative weights of the duodenum, jejunum and ileum has been reported by Steenfeldt (2001). Iji (1999) found that guar gum and xanthin gum significantly increased crypt depth of both the jejunum and ileum, suggesting that NSP may promote GIT cell turnover. The length of the villus is related to the absorption capacity of the enterocytes. Presence of short villi decreases the surface area for nutrient absorption. The epithelial cells of the villi originate in the crypt and a large crypt indicates fast tissue turnover and a high demand for new tissues (Parsaie, 2007). Any additional tissue turnover will increase nutrient requirements for maintenance and will therefore lower the feed efficiency of the animal. Shortening of the villi and deepening crypts can also lead to increased secretion in the GIT, diarrhea, reduced disease resistance and lower overall performance (Parsaie, 2007). Wang et al. (2005) reported a linear decrease in ileal relative length and relative weight on d 21 and d 42 as the level of enzyme supplementation (primarily xylanase and [beta]-glucanase) increased in a broiler wheat-based diet. These studies indicated that NSP-degrading enzymes may counter the negative effects of NSP on intestinal morphology.

In this study, no influence was found on digestive enzyme activities in the small intestine when xylanase was added (Table 5). Engberg et al. (2004) found that whole wheat feeding resulted in lower amylase activity in the pancreatic tissue, whereas xylanase supplementation increased chymotrypsin and lipase activities of broilers. Qian et al. (2004) reported that the feeding of 0.2% [beta]-glucosidase significantly increased intestinal amylase activity, while it had little effect on lipase and trypsin activities of broiler chicks fed corn-soybean meal. Exogenous enzymes release nutrients trapped by the fiber in plant cell walls, causing an increase of substrate in the GIT. The increased activities of digestive enzymes support the hypothesis that birds modulate specific enzymes according to substrate levels, rather than constantly maintaining high enzyme activities (Karasov and Hume, 1997). Almirall et al. (1995) reported that barley reduced amylase and lipase activities in small intestine contents, and that p-glucanase addition increased these activities in broiler chicks, along with a reduction in the intestinal viscosity. However, no changes were observed in adult birds, except for lipase, suggesting that adult birds appear to be able to cope with the intestinal viscosity and digestive enzyme activities are less affected by the diet in adult birds than in young birds (Almirall et al., 1995). In our study, the effects of xylanase on digestive enzyme activities were determined on broilers of 42 d. Thus, the absence of effects may also be associated with the age of broilers.

No effects were observed on the concentration of blood glucose. Gao (2001) reported that xylanase supplementation did not affect plasma glucose concentration, but significantly increased the level of glucose in digesta which indicated that, although the digestion of starch was improved by xylanase, the absorption of glucose was not affected. It is possible that birds modulate glucose absorption to an appropriate level for the needs of metabolism, rather than maintaining a constantly high level of blood glucose. So the results of the present study may be a consequence of the interaction between absorption and metabolism.

The concentration of blood uric acid can accurately reflect the state of protein metabolism and balance of amino acids, and the concentration is low when urea synthesis is reduced by improvement of dietary amino acid profile (Borg et al., 1987). However, the concentration of blood uric acid was not influenced by xylanase addition (Table 6). The possible reason was that amino acid profile in the experimental diets was suitable, and thus an improvement in the utilization of amino acids was not observed.

The growth of birds is modulated by the concentration of hormones such as thyroid hormones, GH, insulin and IGF-I. A close relationship between the somatotropic and thyrotropic axis in regulation of growth and development of broiler chickens has been found to play an important role in poultry growth (Cogburn et al., 1995). Gao et al. (2008) reported that xylanase supplementation to wheat-based diets increased the concentration of blood IGF-I and insulin of 21-day-old broilers, which indicated that enhanced digestion and absorption of nutrients, caused by the the enzyme supplementation, could have an effect on hormone concentrations. In our study, the concentration of insulin and IGF-I was not affected by xylanase supplementation (Table 6). Wang (2004) found that the concentrations of blood thyroxine ([T.sub.4]), [T.sub.3], thyroid stimulating hormone (TSH) and GH were not affected by the supplementation of xylanase to wheat-based diets. The mechanism of exogenous enzyme on hormone regulation is complicated and requires the further study.

In addition, in the present study the excess addition of 5,000 U/kg xylanase had no negative effects on performance, blood hormones, intestinal morphology, microflora and digestive enzyme activities in broilers fed wheat-based diets. The enzyme used in their study was a mixture of digestive (protease and amylase) and non-digestive (xylanase, [beta]-glucanase, pectinase, cellulase and cellobiose) enzymes. Thus, we suggest that the mechanisms of digestive and non-digestive enzymes may not be similar and more studies should be conducted to investigate these mechanisms.

In conclusion, supplementation of xylanase improved the feed:gain ratio of broilers fed wheat-based diets.

Supplementing 500 U/kg and 1,000 U/kg xylanase was beneficial to the morphology of the small intestine. Blood hormones, intestinal microflora and digestive enzyme activities of the intestinal digesta were not affected by xylanase supplementation. Furthermore, excess supplementation of xylanase did not result in further improvement or negative effects on those parameters tested in broilers.


Acamovic, T. 2001. Commercial applications of enzyme technology for poultry production. World's Poult. Sci. J. 57: 225-243.

Ai, X. J., Y. L. Zheng, G. T. Liu and Z. K. Han. 2004. Influences of supplementation of enzyme in rice bran diets on pancrea and chyme activity of young goose. China J. Anim. Husbandry 40: 32-34.

Almirall, M., M. Francesch, A. M. Perez-Vendrell, J. Brufau and E. Esteve-Garcia. 1995. The differences in intestinal viscosity produced by barley and p-glucanase alter digesta enzyme activities and ileal nutrient digestibilities more in broiler chicks than in cocks. J. Nutr. 125: 947-945.

Annison, G. and M. Choct. 1991. Anti-nutritive activities of cereal non-starch polysaccharides in broiler diets and strategies minimizing their effects. World's Poult. Sci. J. 47:222-242.

Bedford, M. R. 2000a. Exogenous enzymes in monogastric nutrition-their current value and future benefits. Anim. Feed Sci. Technol. 86:1-13.

Bedford, M. R. 2000b. Removal of antibiotic growth promoters from poultry diets: Implications and strategies to minimize subsequent problems. World's Poult. Sci. J. 56:347-365.

Bedford, M. R. 2002. The role of carbohydrases in feedstuff digestion. Pages 319-336 in Poultry Feedstuffs-Supply, Composition and Nutritive Value (Ed. J. M. McNab and K. N. Boorman). CABI Publ., England.

Borg, B. S., G. W. Libal and R. C. Wahlstom. 1987. Tryptophan and threonine requirements of young pigs and their effects on serum calcium, phosphorous and zinc concentrations. J. Anim. Sci. 64:1070-1078.

Brenes, A., M. Smith, W. Guenter and R. R. Marquardt. 1993. Effect of enzyme supplementation on the performance and digestive tract size of broiler chickens fed wheat- and barley based diets. Poult. Sci. 72:1731-1739.

Buys, N., C. W. Scheelel, C. Kwakernaakl and E. Decuypere. 1999. Performance and physiological variables in broiler chicken lines differing in susceptibility to the ascites syndrome: 2. Effect of ambient temperature on partial efficiencies of protein and fat retention and plasma hormone concentrations. Br. Poult. Sci. 40:140-144.

Buyse, J., K. Jassens, S. Van der Geyten, P. Van As, E. Decuypere and V. M. Darras. 2002. Pre- and postprandial changes in plasma hormone and metabolite levels and hepatic deiodinase activities in meal-fed broiler chickens. Br. J. Nutr. 88:641-653.

Choct, M., R. J. Huges, R. P. Trimble, K. Angkanaporn and G. Annison. 1995. Non-starch polysaccharide-degrading enzymes increase the performance of broiler chickens fed wheat and low apparent metabolisable energy. J. Nutr. 125:485-492.

Choct, M., R. J. Hughes, J. Wang, M. R. Bedford, A. J. Morgan and G. Annison. 1996. Increased small intestinal fermentation is partly responsible for the anti-nutritive activity of non-starch polysaccharides in chickens. Br. Poult. Sci. 37:609-621.

Cogburn, L. A., N. C. Mao and S. Agarwal. 1995. Interaction between somatotropic and thyrotropic axis in regulation of growth and development of broiler chickens. Arch. Geflugel. 59:9-12.

Cowieson, A. J. 2005. Factors that affect the nutritional value of maize for broilers. Anim. Feed Sci. Technol. 119:293-305.

Engberg, R. M., M. S. Hedemann, S. Steenfeldt and B. B. Jensen. 2004. Influence of whole wheat and xylanase on broiler performance and microbial composition and activity in the digestive tract. Poult. Sci. 83:925-938.

Gao, F. 2001. Effects of non-starch polysaccharides enzyme preparations supplemented to diets on the performances in chicken & pig and the mechanisms involved. Doctor Paper of Nanjing Agricultural University, Nanjing.

Gao, F., Y. Jiang, G. H. Zhou and Z. K. Han. 2008. The effects of xylanase supplementation on performance, characteristics of the gastrointestinal tract, blood parameters and gut microflora in broilers fed on wheat-based diets. Anim. Feed Sci. Technol. 142:173-184.

Hubner, K., W. Vahjen, and O. Simon. 2002. Bacterial responses to different dietary cereal type and xylanase supplementation in the intestine of broiler chicken. Arch. Anim. Nutr. 56:167-187.

Iji, P. A. 1999. The impact of cereal non-starch polysaccharides on intestinal development and function in broiler chickens. World's Poult. Sci. J. 55:375-387.

Iji, P. A., R. J. Hughes, M. Choct and D. R. Tivey. 2001. Intestinal structure and function of broiler chickens on wheat-based diets supplemented with a microbial enzyme. Asian-Aust. J. Anim. Sci. 14:54-60.

Ikegami, S., F. Tsuchihashi, H. Harada, N. Tsuchihashi, E. Nishide and S. Innami. 1990. Effect of viscous indigestible polysaccharides on pancreatic-biliary secretion and digestive organs in rats. J. Nutr. 120:353-360.

Institute of Laboratory Animal Resources Commission on Life Science. 1996. Guide to the care and use of laboratory animals. National Academy Press, Washingtom, DC.

Karasov, W. H. and I. D. Hume. 1997. Vertebrate gastrointestinal system. Pages 409-480 in Handbook of Comparative Physiology (Ed. W. Danstzler). American Physiological Society, Bethesda, MD.

Li, W. F., J. Y. Sun and Z. R. Xu. 2004. Effects of NSP degrading enzyme on in vitro digestion of barley. Asian-Aust. J. Anim. Sci. 17:122-126.

Parsaie, S., F. Shariatmadari, M. J. Zamiri and K. Khajeh. 2007. Influence of wheat-based diets supplemented with xylanas, bile acid and antibiotics on performance, digestive tract measurements and gut morphology of broilers compared with a maize-based diet. Br. Poult. Sci. 48:594-600.

Peng, Y. L., Y. M. Guo and J. M. Yuan. 2003. Effects of xylanase and phytase on the growth and nutrient digestibility in broilers fed wheat-based diets. Acta. Zoonutrimenta Sinica 15:48-52.

Qian, L. C. and J. Y. Sun. 2009. Effects of p-glucosidase as a feed supplementary on the growth performance, digestive enzymes and physiology of broilers. Asian-Aust. J. Anim. Sci. 22:260-266.

SAS Institute. 1996. SAS user's guide: Statistics. Version 7.0. SAS Institute, Cary, NC.

Steenfeldt, S. 2001. The dietary effect of different wheat cultivars for broiler chickens. Br. Poult. Sci. 42:595-609.

Swennen, Q., G. P. J. Janssens, S. Millet, G. Vansant, E. Decuypere and J. Buyse. 2005. Effect of substitution between fat and protein on feed intake and its regulatory mechanisms in broiler chickens: endocrine functioning and intermediary metabolism. Poult. Sci. 84:1051-1057.

Vukic-Vranjes, M. and C. Wenk. 1995. Influence of dietary enzyme complex on the performance of broilers fed on diets with and without antibiotic supplementation. Br. Poult. Sci. 36: 265-275.

Wang, J. Q. 2004. Study on anti-nutritive mechanism of wheat NSP and application of xylanase supplemented in wheat-based diet for broiler. Doctor Paper of the Chinese Academy of Agricultural Science. Beijing.

Wang, Z. R., S. Y. Qiao, W. Q. Lu and D. F. Li. 2005. Effects of enzyme supplementation on performance, nutrient digestibility, gastrointestinal morphology, and volatile fatty acid profiles in the hindgut of broilers fed wheat-based diets. Poult. Sci. 84:875-881.

Yamauchi, K. 2002. Review of chicken intestinal villus histological alterations related with intestinal function. J. Poult. Sci. 39:229-242.

Zhang, W. F., D. F. Li, W. Q. Lu and G. F. Yi. 2003. Effects of isomalto-oligosaccharides on broiler performance and intestinal microflora. Poult. Sci. 82:657-663.

Dingyuan Luo, Fengxia Yanga, Xiaojun Yang, Junhu Yao **, Baojun Shi (1) and Zhenfeng Zhou (1)

College of Animal Science and Technology, Northwest A&F University, Yangling Shaanxi, 712100, China

* This research was supported by Scientific & Technological Innovation Project of Shaanxi, P. R. China (2007ZDKG-15) and the R&D Fund of Guangdong VTR Bio-tech Co. Ltd., Zhuhai, China.

** Corresponding Author: Junhu Yao. Tel: 29-87092102,

Fax: 29-87092164, E-mail:

(1) Guangdong VTR Bio-tech Co. Ltd., Guangdong Zhuhai, 519060, China.

(a) Contribute equal to this paper, as a correspond-first author.

Received January 20, 2009; Accepted April 13, 2009
Table 1. Composition and nutrient levels of the experimental diets

                                       Experiment phase

Ingredient (%)                Growing phase     Finishing phase

Wheat                              40.00             40.00
Corn                               19.00             24.50
Soybean meal                       30.00             22.00
Cottonseed meal                     3.00              5.00
Soy oil                             4.00              4.60
Limestone                           1.24              1.33
Dicalcium phosphate                 1.50              1.30
Salt                                0.30              0.30
Lysine                              0.47              0.47
Methionine                          0.22              0.20
Threonine                           0.02              0.05
Mineral premix (1)                  0.01              0.10
Vitamin premix (2)                  0.04              0.05
Choline-Cl                          0.10              0.10

                                       Experiment phase

Calculated analysis (%) (3)    Growing phase    Finishing phase

ME, kcal/kg                      2,960             3,050
Crude Protein                       22.40             19.80
Ca                                   0.92              0.86
Total P                              0.61              0.56
Nonphytate P                         0.45              0.40
Lysine                               1.35              1.20
Methionine                           0.55              0.50
Methionine+cystine                   0.90              0.82
Threonine                            0.80              0.73

(1) The mineral premix provided per kilogram of diets of 1-21 d and
22-42 d: iron, 100, 60 mg; zinc, 100, 80 mg; copper, 8, 8 mg;
manganese, 120, 60 mg; iodine, 0.7, 0.6 mg; and selenium, 0.3,
0.3 mg, respectively.

(2) The vitamin premix provided per kilogram of diets of 1-21 d and
22-42 d: vitamin A, 8,000, 6,000 IU; vitamin [D.sub.3], 1,000, 500 IU;
vitamin E, 20, 30 IU; menadione, 0.5, 0.5 mg; thiamine, 2.0, 2.0
mg; flavin, 8.0, 5.0 mg; niacin, 35, 30 mg; pyridoxine, 3.5, 3.0
mg; vitamin [B.sub.12], 0.01, 0.01 mg; pantothenic acid, 10.0,
10.0 mg; folic acid, 0.55, 0.55 mg; biotin, 0.18, 0.15 mg;
choline-Cl, 1, 1 g, respectively.

Table 2. Effects of different levels of xylanase supplementation
on the performance of broilers

Parameters                   Dietary enzyme levels (U/kg)

                         0               500              1,000

1-21 d
  BWG (1g)            543              557              557
  ADFI (2g)           930              866              876
  Feed:gain ratio       1.712 (a)        1.556 (b)        1.573 (b)
22-42 d
  BWG (g)           1,382            1,495            1,540
  ADFI (g)          3,043            2,958            3,211
  Feed:gain ratio       2.210            1.979            2.039
1-42 d
  BWG (g)           1,925            2,052            2,098
  ADFI (g)          3,973            3,824            3,998
  Feed:gain ratio       2.068 (a)        1.862 (b)        1.913 (ab)

Parameters          Dietary enzyme    SEM       p
                    levels (U/kg)


1-21 d
  BWG (1g)            549             7       0.556
  ADFI (2g)           866            14       0.164
  Feed:gain ratio       1.577 (b)     0.046   0.013
22-42 d
  BWG (g)           1,462            34       0.155
  ADFI (g)          2,821            67       0.162
  Feed:gain ratio       1.933         0.100   0.065
1-42 d
  BWG (g)           2,011            36       0.137
  ADFI (g)          3,687            69       0.156
  Feed:gain ratio       1.835 (b)     0.075   0.030

(1) BWG means body weight gain. (2) ADFI means average daily
feed intake.

Means in the same row with different superscripts differ
significantly (p<0.05).

Table 3. Effects of different levels of xylanase on the intestinal
microflora composition of broilers at 42 d (log cfu/g)

Parameters               Dietary enzyme levels (U/kg)   SEM    p-value

                          0     500    1,000   5,000

Ileum    E. coli         5.84   5.16   5.56    6.32    0.525    0.062
         Total aerobes   6.51   5.60   6.15    7.54    0.563    0.090
         Lactobacillus   7.43   8.04   8.07    7.59    0.568    0.319

Caecum   E. coli         6.67   7.17   6.95    7.51    0.446    0.108
         Total aerobes   7.19   7.37   7.02    7.64    0.317    0.094
         Lactobacillus   8.03   8.40   7.73    8.18    0.431    0.173

Means in the same row with different superscripts differ significantly

Table 4. Effects of different levels of xylanase on the
small intestine histology of broilers at 21 d ([micro]m)

Parameters                      Dietary enzyme levels (U/kg)

                                     0              500

  Villus height                 876.06 (Cb)     987.78 (ABa)
  Crypt depth                    69.65           67.76
  Villus height: crypt depth     12.62 (Bc)      14.70 (Bbc)
  Epithelial thickness           55.42           39.57
  Muscle thickness              148.64          143.99

  Villus height                 775.89 (BCb)    673.54 (Cc)
  Crypt depth                    50.54           43.94
  Villus height: crypt depth     15.98 (Bb)      15.40 (Bb)
  Epithelial thickness           37.13           26.05
  Muscle thickness              113.00          114.58

  Villus height                 354.82 (Bc)     521.90 (Aab)
  Crypt depth                    51.95           49.91
  Villus height: crypt depth      7.55 (Bb)      10.79 (ABa)
  Epithelial thickness           33.86           29.50
  Muscle thickness              111.39          105.73

Parameters                      Dietary enzyme levels (U/kg)

                                   1,000           5,000

  Villus height                 1020.60 (Aa)    905.44 (BCb)
  Crypt depth                     56.81          61.32
  Villus height: crypt depth      18.02 (Aa)     15.10 (ABb)
  Epithelial thickness            38.13          46.34
  Muscle thickness               137.83         139.05

  Villus height                  975.05 (Aa)    795.40 (Bb)
  Crypt depth                     38.57          48.20
  Villus height: crypt depth      25.53 (Aa)     16.72 (Bb)
  Epithelial thickness            25.55          34.21
  Muscle thickness               110.64         139.53

  Villus height                  556.89 (Aa)    493.82 (Ab)
  Crypt depth                     37.15          44.50
  Villus height: crypt depth      13.74 (Aa)     11.84 (ABa)
  Epithelial thickness            21.27          30.42
  Muscle thickness               104.17         105.53

Parameters                       SEM      p-value

  Villus height                 32.56      0.001
  Crypt depth                    4.78      0.058
  Villus height: crypt depth     1.05      0.001
  Epithelial thickness           6.55      0.067
  Muscle thickness              24.37      0.968

  Villus height                 37.28      0.000
  Crypt depth                    4.51      0.079
  Villus height: crypt depth     1.80      0.000
  Epithelial thickness           4.60      0.052
  Muscle thickness              20.27      0.469

  Villus height                 26.97      0.000
  Crypt depth                    5.90      0.095
  Villus height: crypt depth     1.454     0.000
  Epithelial thickness           4.93      0.986
  Muscle thickness              20.82      0.112

Means in the same row with superscripts of different small and capital
letters differ significantly at p<0.05 and p<0.01, respectively.

Table 5. Effects of different levels of xylanase on amylase
and protease activities in the small intestinal digesta
of broilers at 42 d ([micro]/mg prot.)

Parameters                      Dietary enzyme levels (U/kg)

                          0           500         1,000        5,000

Amylase    Duodenum       62.31        66.99        65.21        61.53
           Jejunum       147.43       142.94       139.75       137.79
           Ileum          38.59        46.77        37.90        36.88

Protease   Duodenum   19,339.75    13,142.60     8,158.72    11,932.43
           Jejunum    32,550.80    34,209.22    28,802.13    30,078.37
           Ileum       8,114.83    16,632.17    15,102.99    12,412.01

Parameters    SEM       p-value

Amylase         5.25     0.708
               25.49     0.983
               10.64     0.779

Protease    4,769.95     0.209
           12,017.25     0.968
            5,911.18     0.529

Table 6. Effects of different levels of xylanase on blood
parameters of broilers

Parameters                   Dietary enzyme levels (U/kg)

                             0       500     1,000    5,000

21 d
  Glucose (mmol/L)         15.61    15.59    16.61    15.13
  Uric acid (mmol/L)        0.40     0.42     0.48     0.34
  Insulin ([micro]IU/ml)   24.79    22.64    20.15    22.79
  IGF-I ([micro]IU/ml)     43.74    41.36    39.73    41.54

42 d
  Glucose (mmol/L)         14.74    15.03    14.06    14.99
  Uric acid (mmol/L)        0.14     0.18     0.21     0.18
  Insulin ([micro]IU/ml)   16.49    17.88    16.42    17.25
  IGF-I ([micro]IU/ml)     41.60    41.89    41.23    38.51

Parameters                 SEM     p-value

21 d
  Glucose (mmol/L)         1.56     0.812
  Uric acid (mmol/L)       0.09     0.506
  Insulin ([micro]IU/ml)   3.16     0.551
  IGF-I ([micro]IU/ml)     2.69     0.539

42 d
  Glucose (mmol/L)         1.08     0.792
  Uric acid (mmol/L)       0.07     0.800
  Insulin ([micro]IU/ml)   2.55     0.931
  IGF-I ([micro]IU/ml)     2.16     0.401
COPYRIGHT 2009 Asian - Australasian Association of Animal Production Societies
No portion of this article can be reproduced without the express written permission from the copyright holder.
Copyright 2009 Gale, Cengage Learning. All rights reserved.

Article Details
Printer friendly Cite/link Email Feedback
Author:Luo, Dingyuan; Yanga, Fengxia; Yang, Xiaojun; Yao, Junhu; Shi, Baojun; Zhou, Zhenfeng
Publication:Asian - Australasian Journal of Animal Sciences
Article Type:Report
Geographic Code:9CHIN
Date:Sep 1, 2009
Previous Article:Effect of inorganic and organic trace mineral supplementation on the performance, carcass characteristics, and fecal mineral excretion of phase-fed,...
Next Article:The effect of clinoptilolite in low calcium diets on performance and eggshell quality parameters of aged hens.

Related Articles
Relief for the digestive health market: with consumers now accepting the fact that a healthy digestive system is crucial to overall well-being, the...
The past, present and future of enzymes: discussing where the enzyme market has been, where it is today and how much it is set to grow in the future.
Xylanase supplementation improved digestibility and performance of growing pigs fed Chinese double-low rapeseed meal inclusion diets: in vitro and in...
Effects of enzyme supplementation on growth, intestinal content viscosity, and digestive enzyme activities in growing pigs fed rough ricebased diet *.
Effects of different levels of supplementary alpha-amylase on digestive enzyme activities and pancreatic amylase mRNA expression of young broilers *.
Effects of supplementing different levels of a commercial enzyme complex on performance, nutrient availability, enzyme activity and gut morphology of...
Effects of xylanase on growth and gut development of broiler chickens given a wheat-based diet.
Corticosterone administration alters small intestinal morphology and function of broiler chickens.
Effects of feed particle size and feed form on growth performance, nutrient metabolizability and intestinal morphology in broiler chickens.
Effect of [beta]-glucosidase as a feed supplementary on the growth performance, digestive enzymes and physiology of broilers.

Terms of use | Copyright © 2017 Farlex, Inc. | Feedback | For webmasters