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Effects of two different deep digital flexor tenotomy techniques on distal articular angles of equine cadaver forelimbs/Efeito de duas tecnicas de tenotomia do flexor digital profundo sobre os angulos articulares distais dos membros anteriores de equinos: estudo post-mortem.

INTRODUCTION

Developmental orthopaedic disease (DOD) and conditions involving the foot are important causes of permanent conformational faults or lameness in horses (KING & MANSMANN, 1997). Among them, flexural limb deformities or 'contracted tendons' and laminitis play an important role in the equine industry scenario not only because of their high morbidity in the equine population but the guarded prognosis for the horse to develop a successful athletic carrier, especially when selected surgical techniques are used as treatment.

A technique employed for years to treat flexural limb deformities of the distal interphalangeal joint as well as laminitis is the radical section of the deep digital flexor tendon (DDFT) (FACKELMAN et al., 1983). Two different surgical approaches have been described for performing the procedure. In the first one, the DDFT is exteriorized and severed at the mid-metacarpal level (ADAMS & FESSLER, 2000). In the second one, the tendon is exteriorized and severed following opening of the tendon sheath at the mid-pastern level (FACKELMAN et al., 1983). Apart from the advantages and disadvantages inherent to each technique, immediate and sometimes unwanted effects (e.g. hyperextension) are produced by the radical section of the tendon (HUNT et al., 1991; FACKELMAN et al., 1983; KRAMER, 2006). Although clinical observations have suggested that 'release of the tendon' to a greater extent is produced by the pastern technique (HUNT et al., 1991; HUNT, 2003), quantitative data regarding the distal conformational modifications induced by either technique is lacking.

One of the difficulties encountered to study the tenotomy's effects in live and healthy animals is the ethical and animal welfare considerations related to the crippling nature of the technique. In order to overcome these issues, biomechanical systems capable of generating forces applied to the limb using ex-vivo specimens have been successfully developed allowing extrapolations of the results to clinical situations (BUKOWIECKI et al., 1987; SHOEMAKER et al., 1991; ALEXANDER et al., 2001). To the authors' knowledge, controlled studies comparing the effects of both techniques using biomechanical models have not been described.

Therefore, the purpose of the present study was to investigate the viability of a proposed biomechanical system of induced-traction used to compare the two deep digital flexor tenotomy techniques currently employed in clinical practice by measuring the distal articular angles of equine cadaver forelimbs. The tested hypothesis was that the pastern technique would produce a greater 'release of the tendon' when compared to the mid-metacarpal technique.

MATERIAL AND METHODS

Procedures were performed in cadaver forelimbs of horses that were euthanatized for reasons unrelated to the project or died in the premises of the Veterinary Teaching Hospital of the State University of Londrina, Parana, Brazil. Pairs of forelimbs from 10 mixed breed horses, aged 8 months to 12 years, with no signs of musculoskeletal disorders were collected immediately after death and frozen at -20[degrees]C until the time of testing. Body weight ranged approximately from 280 to 500kg. The entire forelimb, from the scapula to the foot, was used. Following complete thawing at room temperature, limbs were transversally drilled at selected sites and secured with their medial aspect facing down to a 180cm x 30cm x 2.5cm wooden platform by means of 7mm , full threaded bolts with nuts and washers. Bolts were placed at the proximal and distal aspect of the third metacarpal bone, proximal radius and proximal scapula allowing free movement of the hoof and distal joints. An approximately 5cm x 5cm x 2cm drilled wooden block was interposed between the medial aspect of the limb and the platform at screw sites to allow slight elevation of the limb from the platform. A round, full threaded, 8cm long, 5mm [empty set] screw hook was perpendicularly drilled to the distal aspect of the hoof wall at midline (toe level) and fixed at the sole aspect with a nut and washer. On the dorsal aspect of the limb a digital scale was connected to the screw hook. The opposite extremity of the scale was connected to a 2mm [empty set] steel cable positioned parallel to the limb axis up to a turnbuckle attached to the top of the platform (Figure 1). A 20kg tension was applied to the system by adjusting the turnbuckle. A radiographic exam (lateromedial projections) of the metacarpophalangeal (MP), proximal interphalangeal (PIP) and distal interphalangeal (DIP) joints was performed and the articular angulations were determined by geometric lines traced along the longitudinal axis of each bone as described by HUSSNI (1991). Briefly, the longitudinal axes (bisecting lines) of the third metacarpal bone, proximal and middle phalanx were drawn by a line uniting two midpoints on the diaphyses of each bone. The longitudinal axis of the distal phalanx was drawn by a line traced parallel to its dorsal surface. The dorsal angles formed by the intersected lines were measured with the aid of a protractor. The experimental model was designed for each limb to act as its own control. Therefore, angles initially obtained from the limbs were assigned to a control group. Once the initial radiographic exam was concluded, the tension on the turnbuckle was released and one of the two deep digital flexor tenotomy techniques (i.e. mid-metacarpal or pastern) was randomly (coin toss) selected and performed according to FACKELMAN et al. (1983) and ADAMS & FESSLER (2000), respectively. Shortly, for the mid-metacarpal technique, a 4cm longitudinal skin incision was made over the lateral aspect of the DDFT followed by blunt dissection of the tendon. The DDFT was exteriorized and transversally severed. For the pastern technique, a 4cm longitudinal skin incision was made on the palmar aspect of the pastern, the tendon sheath was incised and the DDFT was transversally severed. Once the technique was completed, the same tension was reapplied to the system and a second radiographic exam was performed. Measurements of the dorsal joint angles were repeated as previously described. The second technique was performed on the remainder limb of the pair following the same procedures previously described. Angles obtained from the limbs subjected to the mid-metacarpal technique were designated as "metacarpal tenotomy (mc)" group. Similarly, angles obtained from the limbs subjected to the pastern technique were designated as "pastern tenotomy (p)" group. The author responsible for measuring the angles on radiographs was unaware of the employed technique.

[FIGURE 1 OMITTED]

Data were analyzed using Minitab for Windows (Minitab Inc. USA). Normality of data was assessed by the Ryan-Joiner (similar to Shapiro-Wilk) normality test. For each joint, comparisons between each tenotomy group and its own control group were analyzed by the Student t-test for pairwise samples. Comparisons between the two tenotomy groups were analyzed by the Student t-test using as variable the difference between the tenotomy and control groups. The significance level of the test was set at 0.05.

RESULTS AND DISCUSSION

Assembly of the induced-traction construct didn't present any difficulty. The whole limb, from the scapula to the hoof, was used to preserve all proximal attachments of the deep digital flexor muscle (i.e. medial umeral epicondile, medial aspect of the olecranon, caudal aspect of the radius and small portion of the ulna) (SISSON, 1981) and its anatomical relationship with adjacent muscle groups.

Significant differences (P [less than or equal to] 0.01) between control and tenotomy groups regardless of the technique employed were noticed for each joint. The dorsal articular angles of the MP and DIP joints decreased to a greater extent when compared to the PIP joint (Table 1). Primary stability of PIP joint is provided by two short collateral ligaments and four palmar ligaments (KAINER, 2002). The reason the dorsal articular angle of the PIP joint decreased to a lesser extent may be explained by the strong stability of the joint provided by the palmar support structures other than the DDFT. As previously stated, overextension of the PIP joint is prevented by its palmar (axial and abaxial) ligaments, the straight sesamoidean ligament as it inserts on the scutum medium and the SDFT (DENOIX, 1994) through its insertions on the distal and proximal extremities of the proximal and middle phalanx, respectively (SISSON, 1981; KAINER, 2002).

The dorsal MP joint angle was the most affected by the DDF tenotomy. Differences up to 23 degrees could be observed between control and tenotomy groups (Table 1). In clinically normal horses, when joints are loaded in axial compression during the weight bearing position, deep digital flexor tenotomy has its greatest effects over the DIP joint angulation (HUSSNI, 1991). The loading pattern underwent by the limbs in the present study was developed by upward tension at the toe level. When modifications of the toe or heel angle are experimentally induced in live horses or in isolated limbs, corresponding modifications occurs on distal joint angulations and on tension of the flexor tendons and suspensory apparatus (DENOIX, 1994; RIEMERSMA et al., 1996; CREVIERDENOIX et al., 2001). Elevation of the toe results in DIP joint extension with increased tension on the DDFT and its accessory ligament, straightened up of the pastern and increased dorsal MP angle. Furthermore, participation of the SL and SDFT in supporting the fetlock is decreased (Denoix, 1985, 1987 apud DENOIX, 1994). Although the toes of the control limbs used in this experiment were not intentionally tensioned to a point of rotating upward (mean DIP angle = 159.7[degrees]), the tension used (i.e. 20kg) might have caused a certain degree of toe elevation, especially in the specimens taken from lighter horses. This observation is supported by the DIP joint angle (mean [+ or -] sd = 171.6[degrees] [+ or -] 5.99) described in 20 normal horses (HUSSNI, 1991). In these horses, the mean [+ or -] sd MP joint angle was 154.27 [+ or -] 6.5 (HUSSNI, 1991). Therefore, the increased dorsal MP angle induced by traction at the toe (mean [+ or -] sd = 171.7 [+ or -] 3.36) might have played a central role in the angular differences of the MP joint between clinically normal horses and the specimens used in the experiment reported here.

Comparisons between the pastern and the mid-metacarpal technique didn't show any statistical significance for any joint. The lack of statistical difference was probably due to the small sample size (power = 0.307). Thus, a larger sample size should have been used to result in significant differences between groups. Despite the lack of statistical difference, the pastern technique has produced extension of the DIP joint to a greater extent when compared to the mid-metacarpal technique (Figure 2), confirming the clinical impression. It has been reported that the magnitude of tension release provided by the pastern technique is greater when compared to the mid-metacarpal technique. Tendon ends may separate 6 to 10 cm after transection in the pastern region (HUNT, 2003). Although separation between the transected tendon ends was not measured in this study, the overall results are in agreement with the observations reported by HUNT (2003). According to his observations, this greater release would be achieved because of the close proximity of the surgical region to the insertion of the DDFT on the distal phalanx and unrestricted movement within the deep flexor tendon sheath. In contrast, separation after mid-metacarpal tenotomy would be restricted by peritenon attachment to the subcutaneous tissue (HUNT, 2003; PARKS & O'GRADY, 2003). An alternative hypothesis to explain the lesser tendon release produced by the mid-metacarpal technique would be the greater compression sustained by the distal segment of the DDFT at the fetlock joint after transection. The increased fetlock extension produced by the DDF tenotomy, which has been demonstrated in this study and elsewhere (HUSSNI, 1991), would create increased dorso-palmar pressure over the DDFT against the SDFT. At this level, both flexor tendons are palmarly surrounded by the inelastic, strong, transverse fibers of the palmar annular ligament, which in association with the proximal scutum (i.e. proximal sesamoid bones and intersesamoidean ligament) form a real canal (DENOIX, 1994). The resultant increase in transverse compression at the fetlock level over the distal segment of the transected DDFT would ultimately restrict its distal excursion. The lesser the distal excursion of the distal DDFT segment, the lesser the DIP joint extension.

[FIGURE 2 OMITTED]

One of the disadvantages of the deep digital flexor tenotomy technique regardless of what region it is performed is a variable degree of digital hyperextension (HUNT et al., 1991; FACKELMAN et al., 1983; KRAMER, 2006) that can occur following the procedure. Based on the results of this study and the degree of contracture of the distal interphalangeal joint observed in a given horse, it is possible that digital hyperextension can be null or produced to a lesser extent when the deep digital flexor tenotomy is performed at the mid-metacarpal level. Grounds for this though are supported by a study comparing the effects of the deep digital flexor tenotomy performed proximal and distal to the insertion of the inferior check ligament on the DDFT on normal horses. Out of 10 horses, in which the procedure was performed at the mid-metacarpal level distad to the insertion of the inferior check ligament, hyperextension was observed permanently in 3 horses, only at walk in 4 horses and only at a trot in 3 horses (HUSSNI, 1991).

Interestingly, the greater extension produced in the DIP joint by the pastern technique was not uniformly observed in all joints. A slight increase in extension of the MP joint produced by the mid-metacarpal technique was noted when compared to the pastern technique. Again, this difference could have been more reliable if a larger sample size had been used.

Although the construct used in this experiment to compare the two deep digital flexor tenotomy techniques has provided valuable estimations regarding the quantitative differences of distal joint angulations between both techniques, the study is not exempt from limitations. Similarly to other biological variables, measurement of joint angles radiographically represented by the longitudinal axes of bones composing a joint is a variable amenable to imprecision. Although it has been used and validated in previous experimental studies (BUSHE et al., 1987; HUSSNI, 1991), small variations in joint angulations, particularly due to a slight degree of obliqueness of the radiographs (BUSHE et al., 1987) should not be unexpected.

The idea behind the development of the traction-induced biomechanical system was based upon the ethical and animal welfare considerations related to the crippling nature of the technique. Although the system has not been able or developed to reproduce the pattern of loading or conditions observed in clinical situations, it is believed that good estimations of the distal joints angle range can be obtained by it when tenotomy or even other selected corrective techniques (e.g. desmotomy) are performed in clinical patients. A system that could mimic a more realistic scenario of forces such as one mounted exerting axial compression forces from top to bottom of the limb rather than applying traction on its distal aspect should provide more precise information.

In conclusion, the DDF tenotomy technique at the pastern level performed in cadaver forelimbs subjected to a traction-induced biomechanical system didn't produce statistically significant differences on the distal joint angles when compared to the mid metacarpal level. The lack of statistical difference is probably related to the small sample size. From the clinical standpoint, these results emphasize the need of a proper case selection before indication of either technique.

ACKNOWLEDGMENTS

The authors would like to thank the radiology technician Mr. Antonio Ribeiro Guimaraes for performing the radiographic exams, the State University of Londrina Universidade Estadual de Londrina--for the undergraduate scholarship in science (PROIC--UEL) and the Araucaria Foundation--Fundacao Araucaria (Apoio ao Desenvolvimento Cientifico e Tecnologico do Parana)--for funding the project.

REFERENCES

ADAMS, S.B.; FESSLER, J.F. Deep digital flexor tenotomy. In:--. Atlas of equine surgery. Philadelphia: Saunders, 2000. Cap.73, p.359-361.

ALEXANDER, GR. et al. Effects of superior check desmotomy on flexor tendon and suspensory ligament strain in equine cadaver limbs. Veterinary Surgery, v.30, p.522-527, 2001. Available from: <http://onlinelibrary.wiley.com/doi/10.1111/vsu.1991.20.issue-1/issuetoc>. Accessed: August 3rd, 2011. doi: 10.1111/j.1532-950X.1991.tb00300.x.

BUSHE, T. et al. The effect of hoof angle on coffin, pastern and fetlock joint angles. Proceedings of the American Association of Equine Practitioners, v.33, p.729-737, 1987. Available from: <http://agris.fao.org/agris-search/search/display.do?f=1989/US/US89513.xml;US8843016>. Accessed: August [3.sup.rd] 2011.

BUKOWIECKI, C.F. et al. In vitro strength of the suspensory apparatus in training and resting horses. Veterinary Surgery, v.16, p.126-130, 1987. Available from: <http://onlinelibrary.wiley.com/doi/10.1111/vsu.1987.16.issue-2/issuetoc>. Accessed: August 3rd, 2011. doi: 10.1111/j.1532950X.1987.tb00923.x.

CREVIER-DENOIX, N. et al. Effects of heel and toe elevation upon the digital joint angles in the standing horse. Equine Veterinary Journal, v.33, Suppl.33, p.74-78, 2001. Available from: <http://onlinelibrary.wiley.com/doi/10.1111/ evj.2001.33.issue-S33/issuetoc>. Accessed: August [3.sup.rd], 2011. doi: 10.1111/j.2042-3306.2001.tb05364.x.

DENOIX, J.M. Functional anatomy of tendons and ligaments in the distal limbs (manus and pes). Veterinary Clinics of North America: Equine Practice, v.10, n.2, p.273-322, 1994. Available from: <http://www.ncbi.nlm.nih.gov/pubmed/7987720>. Accessed: August 4th, 2011.

FACKELMAN, G.E. et al. Surgical treatment of severe flexural deformities of the distal interphalangeal joint in young horses. Journal of the American Veterinary Medical Association, v.182, p.949952, 1983. Available from: <http://www.ncbi.nlm.nih.gov/pubmed/6682845>. Accessed: August [4.sup.th], 2011.

HUNT, R.J. et al. Mid-metacarpal deep digital flexor tenotomy in the management of refractory laminitis in horses. Veterinary Surgery, v.20, n.1, p.15-20, 1991. Available from: <http://www.ncbi.nlm.nih.gov/pubmed/2008769>. Accessed: August [4.sup.th], 2011.

HUNT, R.J. Laminitis. In: ROSS, M.W.; DYSON, S.J. Diagnosis and Management of lameness in the horse. Philadelphia: Saunders, 2003. Cap.35, p.325-339.

HUSSNI, C.A. Tenotomia do musculo flexor digital profundo proximal a insercao do ligamento acessorio: proposta de uma nova tecnica cirurgica em equinos e seu efeito sobre os angulos das articulacoes metacarpofalangeana e interfalangeanas. 1991. 37f. Dissertacao (Mestrado em Medicina Veterinaria)--curso de Pos-graduacao em clinica: fisiopatologia medica, FMVZ, Unesp, Botucatu, SP.

KAINER, R.A. Functional anatomy of equine locomotor organs. In: Stashak, T.S. Adams' lameness in horses. 5.ed. Philadelphia: Lippincott Williams & Wilkins, 2002. Cap.1, p.1-72.

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KRAMER, J. Deep digital flexor tenotomy. In: Wilson, D. et al. Manual of equine field surgery. St. Louis: Saunders Elsevier, 2006. Cap.16, p.98-102.

PARKS, A.; O'GRADY, S.E. Chronic laminitis: current treatment strategies. Veterinary Clinics of North America: Equine Practice, v.19, p.393-416, 2003. Available from: <http://www.ncbi.nlm.nih.gov/pubmed/14575166>. Accessed: August [4.sup.th], 2011.

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Antonio Cezar de Oliveira Dearo (I) Vitor Bruno Bianconi Rosa (II) Peter Reichmann (I) Milton Luis Ribeiro de Oliveira (I)

(I) Departamento de Clinicas Veterinarias, Centro de Ciencias Agrarias (CCA), Universidade Estadual de Londrina (UEL), CP 6001, 86051-990, Londrina, PR, Brasil. E-mail: dearoaco@uel.br. Autor para correspondencia.

(II) Programa de Residencia em Clinica Medica, Cirurgica e Reproducao de Grandes Animais, UEL, Londrina, PR, Brasil.

Received 08.08.11 Approved 06.14.12 Returned by the author 08.06.12 CR-5796
Table 1--Descriptive statistics of joint angles (in degrees)
of equine cadaver forelimbs subjected to different DDF
tenotomy techniques in an induced-traction construct.

Measure    Technique          Control group

                                 Joints

                           MP       PIP       DIP

Mean          mc        171.2       166     159.7
               p        172.2     166.1     160.6
SEM           mc         0.57      1.39      1.34
               p         1.41      0.94      1.77
SD            mc         1.81      4.40      4.24
               p         4.47      2.96      5.58

Measure           Tenotomy group

                     Joints

               MP       PIP       DIP

Mean        157.6     163.3       154
            158.7     163.2     151.1
SEM          1.27       1.0      1.09
             1.41      0.57      1.18
SD           4.01      3.16      3.43
             4.47      1.81      3.73

Measure    Differences (Control--Tenotomy)

                     Joints

               MP       PIP       DIP

Mean         13.6       2.7       5.7
             13.5       3.0       9.5
SEM          1.02      0.83      0.86
             1.69      0.76      1.85
SD           3.24      2.63      2.71
             5.36      2.40      5.84

MP = Metacarpophalangeal

PIP = Proximal interphalangeal

DIP = Distal interphalangeal

SD = Standard deviation

SEM = Standard error of the mean

mc = Mid-metacarpal

p = Pastern
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Author:Dearo, Antonio Cezar de Oliveira; Rosa, Vitor Bruno Bianconi; Reichmann, Peter; de Oliveira, Milton
Publication:Ciencia Rural
Date:Nov 1, 2012
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