Effects of non-ionic polymers of surfactant additives on stress induced apoptosis in type II pneumocytes.
The exposure of the cells to hyperoxia/etoposide/ UV could result in increased apoptosis. Presence of surfactant resulted in a decrease in the percentage of apoptotic cell death in all the cases. Further, the extent of apoptosis was reduced in the cells exposed to hyperoxia/UV in presence of PEG/dextran alone or along with surfactant. However, an increase in the percentage of apoptotic cell death was noticed in case of cells exposed to etoposide in presence of PEG/dextran alone. These results were further substantiated by cell death detection ELISA. Formation of the nucleosomal DNA ladder is the hallmark of apoptosis so analysis of the pattern of DNA isolated from the cells exposed to different stress inducers was done. The ladder like appearance of the fragmented DNA was quite prominent in cells exposed to [O.sub.2] for 8h. No such ladder was observed in the control cells. Very faint ladder was detected in the cells exposed to hyperoxia in presence of surfactant/nonionic polymers like PEG/dextran. Exposure of cells to etoposide/UV also resulted in formation of ladder which was not very prominent in these cells in presence of surfactant/ nonionic polymer. Further, the oxygen exposed cells and the cells exposed to etoposide/UV showed maximum FITC-dUTP end labeling which pointed to the higher percentage of apoptosis in these cells as compared to the control cells and also the cells cotreated with the modulators.
Western blotting was performed to assess the expression level of Bcl-XI (antiapoptotic protein. and Bax (proapoptotic protein., which revealed a decreased expression of Bcl-XI in the stress (hyperoxic, etoposide/ UV. exposed cells. Bcl-XI expression was comparably same in the control cells and also the cells exposed to stress in the presence of modulators like surfactant or nonionic polymer. Expression of proapoptotic protein Bax was found to increase in the cells exposed to hyperoxia or other stress inducers as compared to the control cells.
Professor & Head
Department of Experimental
Medicine & Biotechnology
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|Title Annotation:||ABSTRACTS: Some Research Projects Completed Recently|
|Date:||Oct 1, 2007|
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