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Effects of feeding barley naturally contaminated with fusarium mycotoxins on growth performance, nutrient digestibility, and blood chemistry of gilts and growth recoveries by feeding a non-contaminated diet.

INTRODUCTION

There is growing concern for mycotoxins on swine production as they can cause reduced growth and feed intake, organ damage, and altered immune responses of pigs (Chaytor et al., 2011). Among various mycotoxins, Fusarium mycotoxins are secondary toxic metabolites produced by various species of the genus Fusarium such as F. graminearum and F. culmorum and frequently found in various feedstuffs for swine diet thus have received considerable attention (CAST, 2003; Chaytor et al., 2011). Deoxynivalenol (DON) and zearalenone (ZON) are the most important Fusarium mycotoxins in terms of frequency of occurrence as well as concentration of toxins relative to guidance level (CEC, 2006; Rodrigues and Naehrer, 2012a).

Because pigs are known to be very susceptible to DON (Prelusky et al., 1994), several studies were conducted to find feed additives ameliorating the detrimental effects of DON but their effects are still equivocal (Papaioannou et al., 2002; Weaver et al., 2013; Xiao et al., 2013; Kong et al., 2014). The most apparent signs of DON intoxication are reduced feed intake and consequent body weight (BW) loss of pigs. The DON also modulates immune responses as well as affects blood chemistry in pigs (Bergsjo et al., 1993; Swamy et al., 2002; Danicke et al., 2004b). The ZON known as a mycoestrogen and estrogenic properties of its metabolites have been reported to cause reproductive disorders by hyperestrogenism (Takemura et al., 2007; Kanora and Maes, 2009; Jiang et al., 2010).

Barley ranks in fifth in global grain production (USDA, 2014). Although energy content in barley is about 10% to 15% less than that in corn, barley is still a very attractive ingredient for swine diet because of higher contents of crude protein (CP), lysine, and digestible phosphorus compared with corn (NRC, 2012; Woyengo et al., 2014). In addition, feeding diets containing barley up to 60% at the expense of corn did not show any detrimental effects on animal performances (Kim et al., 2014). A global survey for the incidence of mycotoxins in various feedstuffs indicated that the positive occurrences of DON and ZON in barley were 42% and 38%, respectively, and the average concentrations of DON and ZON were 1.677 and 0.323 mg/kg which are greater than the suggested guidance levels of mycotoxins for swine diets at 0.9 and 0.1 mg/kg, respectively (CEC, 2006; Rodrigues and Naehrer, 2012b). Moreover, it was reported that the effect of Fusarium mycotoxins on growth performance of pigs was dependent on the source of mycotoxin (i.e. purified vs naturally contaminated) in ingredient (Prelusky et al., 1994). However, little data from study feeding contaminated barley with Fusarium mycotoxins to pigs is available. Therefore, the objectives of the present study were to investigate the effects of feeding barley naturally contaminated with Fusarium mycotoxins on growth performance, vulva swelling, and digestibility of dry matter (DM), organic matter (OM), and CP for gilts and the recovery of gilts fed normal diets immediately after the exposure to the contaminated diets by measuring growth performance and vulva size.

MATERIALS AND METHODS

All the experimental procedures used in the current study were approved by the Institutional Animal Care and Use Committee at Konkuk University.

Exp. 1

Animals and experimental design: In a 14-d experiment, 16 crossbred gilts with an average initial BW of 33.3 kg (standard deviation = 3.0) were individually housed in metabolism crates (0.48 x 1.49 m) in an environmentally controlled room. The pigs were allotted to 4 dietary treatments in a randomized complete block design with 4 blocks based on BW.

Ingredients, diets, feeding, and sample collection: The concentration of DON in the corn, soybean meal (SBM), control barley, or naturally contaminated barley used were analyzed to be 0.6, 1.0, 0.3, or 25.7 mg/kg, respectively, and the concentrations of ZON were less than the detection limit (25.0 [micro]g/kg) except for contaminated barley which was analyzed to be 26.0 [micro]g/kg (Table 1).

A corn-SBM based diet containing 45% of control barley was prepared and 3 additional diets were also formulated to contain 15%, 30%, or 45% of the contaminated barley at the expense of the control barley (Table 2). Individual BW and feed consumption were measured on d 0, 7, and 14 and at the completion of experiment on d 14 average daily gain (ADG), average daily feed intake (ADFI), and gain:feed (G:F) were calculated. Individual vertical and horizontal lengths of vulva were measured on d 0, 3, 6, 9, and 12. From d 10 to 14, fecal samples were collected quantitatively by the marker-to-marker method as described by Kong and Adeola (2014). Blood samples were collected from all the pigs at the completion of study on d 14.

Mycotoxins and chemical analyses: The concentration of DON and ZON in the ingredients and diets were determined by using enzyme-linked immunosorbent assay kits (AgraQuant, Romer Labs Inc., Singapore, Republic of Singapore) which had quantification ranges for analysis on DON and ZON from 250 to 5,000 and 25 to 1,000 ng/mL, respectively.

All the fecal samples and orts collected for each gilt were dried in a forced-air oven at 55[degrees]C to constant weight and ground using a coffee grinder. Dry matter analysis of samples was performed by drying the samples in a forced-air oven at 135[degrees]C for 2 h (method 930.15; AOAC, 2005). Nitrogen content of samples was determined by the Kjeldahl method (method 984.13; AOAC, 2005) using a Buchi K-424 digestion and B-324 distillation apparatus (Buchi, Flawil, Switzerland) and gross energy was determined by adiabatic bomb calorimeter (Parr 1261 bomb calorimeter; Parr Instruments Co., Moline, IL, USA) using benzoic acid as a calibration standard. Serum concentration of immunoglobulin subsets (IgA, IgG, and IgM) was determined on a Cobas Integra 800 analyzer (Roche, Mannheim, Germany). An automated blood chemistry analyzer (Roche Cobas c702; Roche, Germany) was used to measure the amounts of albumin, globulin, alkaline phosphatase (ALP), Ala transaminase (ALT), Asp transaminase (AST), [gamma]-glutamyl transferase (GGT), blood urea nitrogen (BUN), calcium, glucose, phosphorus, and total protein in the serum samples.

Exp. 2

Diets and feeding: A 14-d feeding trial was conducted to evaluate the recovery of pigs that previously consumed diets contaminated with mycotoxin in Exp. 1. Immediately after ending of Exp. 1, the pigs were fed a corn-SBM-based standard diet (Table 3) which met or exceed the nutrient requirement estimates of pigs (NRC, 1998). Individual pig BW and feed consumption were measured on d 0, 7, and 14 and the vertical and horizontal lengths of vulva were measured every 3 days from d 0 to 14.

Calculations and statistical analyses

For each of the experimental diets, the apparent total tract digestibility of DM, OM, and CP was determined according to the total collection method as described by Kong and Adeola (2014).

The experimental data were statistically analyzed using MIXED procedures of SAS (SAS Inst. Inc., Cary, NC, USA). The independent variables in the model included diet as a fixed effect and block as a random effect. Growth performance including ADG, ADFI, and G:F, immune responses and blood characteristics were response variables and each animal was considered as an experimental unit. Orthogonal polynomial contrasts were used to show linear or quadratic responses to the level of contaminated barley in the experimental diets and specific orthogonal contrasts were also used to compare the recovery results between pigs previously fed diet without and with contaminated barley. The alpha level used for the determination of statistical differences was set at 0.05. Probability values between 0.05 and 0.10 were considered tendencies.

RESULTS

Exp. 1

Mycotoxin analysis: The concentrations of DON and ZON in diets containing 0%, 15%, 30%, or 45% contaminated barley were analyzed to be 0.6, 6.1, 7.7, or 14.6 mg/kg and 23.8, 35.4, 47.8, or 33.7 [micro]g/kg, respectively (Table 2).

Growth performance and vulva size: From d 0 to 7, the ADFI for pigs fed increasing level of the contaminated barley in the diets showed linearly decreasing trend (p = 0.056) and the ADG and G:F decreased linearly (p = 0.011) as the contaminated barley levels increased (Table 4). From d 7 to 14, the ADG and ADFI of pigs linearly decreased (p<0.01) with increasing level of the contaminated barley in the diets whereas the G:F of pigs showed linearly decreasing trend (p = 0.063). On d 14, the final BW of pigs fed diets with increasing level of the contaminated barley linearly decreased (p = 0.017). During the overall period, all growth performance measurements linearly decreased (p<0.01) as the level of contaminated barley in the diets fed to pigs increased.

The vulva size of pigs fed the diets with increasing level of contaminated barley was not affected throughout the 12-d period (Table 5).

Digestibility: The daily feed DM, OM, and CP intake as well as the respective feces components output linearly decreased (p<0.01) as the level of the contaminated barley in the diets increased (Table 6). However, the CP digestibility linearly increased (p = 0.011), and the digestibility of DM (p = 0.065) and OM (p = 0.060) showed increasing trend as the contaminated barley in the diets increased.

Serum immunoglobulin and blood characteristics: The concentrations of serum IgA, IgG, and IgM were not influenced by the increase of the contaminated barley in diets fed to pigs (Table 7). Feeding of increasing level of the contaminated barley to pigs linearly increased BUN (p = 0.016) whereas other blood chemistry measurements were not affected.

Exp. 2

Growth performance: From d 0 to 7, the ADG and G:F of pigs previously fed 15% or 45% of the contaminated barley in diets was greater (p<0.05) compared with pigs previously fed the control barley in diet (Table 8). No difference was observed in the growth performance among the pigs previously fed the diets containing 0%, 15%, 30%, or 45% of contaminated barley from d 7 to 14. For the overall period, the ADG of pigs previously fed 15% or 45% of the contaminated barley in diets was greater (p<0.05) compared with the pigs previously fed the control barley diet.

Vulva size: During the recovery period, the vulva size measured was not influenced by the levels of the contaminated barley in diets fed previously (Table 9).

DISCUSSION

Exp. 1

The difference between the analyzed and calculated concentrations of mycotoxins in the experimental diets based on the analyzed values of the individual ingredient was observed in the present study. This is in agreement with Swamy et al. (2003) who also observed much higher analyzed values of DON compared with the intended values in diets. This might be attributed to uneven distribution of mycotoxins in the ingredients used. The analyzed mycotoxin also showed that the contaminated barley used in the present study was primarily contaminated with DON rather than ZON.

Increasing level of contaminated barley with Fusarium mycotoxins negatively affected growth performance of growing pigs. Feed intake depression and consequent decrease of BW gain were commonly observed and are considered as the major adverse effects of feeding DON-contaminated diets to pigs (Swamy et al., 2003; Goyarts et al., 2005; Mok et al., 2013) and these effects were also observed in the present study. Moreover, the reductions in ADG of pigs fed diet containing 45% contaminated barley compared with diet containing 0% contaminated barley from d 0 to 7, 7 to 14, and the overall period, were 90.3%, 70.3%, and 80.4%, respectively, which were close to the value (93.8%) predicted by the equation derived from literature data on growth performance of pigs exposed to various levels of DON (Mok et al., 2013). Considering the guidance levels of DON and ZON for swine diets at 0.9 and 0.1 mg/kg, respectively (CEC, 2006), the concentrations of DON in the contaminated diets were above the guidance level while those of ZON were not. In addition, the vulva swelling which is one of common clinical symptoms of pigs exposed to ZON was not observed in the present study. Therefore, it might be speculated that the poor growth performance was primarily attributed to the presence of DON rather than ZON in contaminated diets fed to pigs. However, other unanalyzed Fusarium toxins which might interact with DON could not be excluded as a contributor for the poor growth performance. There was a linear decrease in the G:F of pigs fed diets with increasing levels of contaminated barley with Fusarium mycotoxins from d 0 to 7 during the first week as well as the overall period and this is in accordance with Young et al. (1983) and Smith et al. (1997) who also reported a linear reduction in the G:F as dietary DON increased in the diets. On the contrary, other studies observed a significant depression of feed intake and BW gain but no effect (Swamy et al., 2002) or an increase (Rotter et al., 1994; Swamy et al., 2003) in feed efficiency. This conflict of results among experiments may be, in part, attributed to different physiological stage of pigs (age, sex, or BW), source of contaminations (type of grains), and concentration of mycotoxins (Goyarts et al., 2005).

In contrast to the adverse effects of feeding diets contaminated with Fusarium mycotoxins on the growth performance, the positive effect was shown for the digestibility of DM, OM, and CP. This was in agreement with Danicke et al. (2004a) who also found a DON-related improvement in nutrient digestibility. Several explanations could be possible for this positive effect. Matthaus et al. (2004) found that there were increased activities of protease, amylase, and non-starch polysaccharide-degrading enzymes in the wheat inoculated with Fusarium culmorum compared with the control wheat and were alterations of the cell penetrating properties by fungus. Reduced feed intake due to the presence of mycotoxin might also be attributed to the increase in nutrient utilization because Haydon et al. (1984) observed that the apparent total tract digestibility of DM and energy tended to increase as the feed intake of 25-kg pigs fed sorghum-SBM based diet decreased. However, Danicke et al. (2004a) and Goyarts et al. (2005) reported that significant increases in nutrient digestibility for pigs fed diets contaminated with DON regardless of the feeding methods (i.e. ad libitum vs restricted) indicating that levels of feed intake did not affect nutrient digestibility.

Blood urea nitrogen is an indication of renal health and a linear increase of BUN was observed but the values were within the reference range of BUN in pigs (8.2 to 25 mg/dL; Latimer et al., 2003). Signs of liver damage (ALP, ALT, AST, and GGT) were not altered as the concentration of mycotoxin in the diets increased. Similarly, no signs of liver damage were detected in previous studies reported (Danicke et al., 2004a; Accensi et al., 2006) whereas other study reported that there was a dose-response related decrease in blood measurements for the sign of liver damage (Doll et al., 2003). Two major groups of blood total proteins are albumin and globulin, and their alterations are indicatives of liver damage and abnormal immune functions, respectively (Busher, 1990). In some experiments, increases of serum albumin:globulin ratio were observed in pigs fed diets containing Fusarium mycotoxins (Rotter et al., 1994; Swamy et al., 2003). However, there were no changes in albumin:globulin ratio as well as total protein with increasing level of contaminated barley in experimental diets used in the present study. Rotter et al. (1994) suggested that DON and other Fusarium mycotoxins might directly affect globulin synthesis in the liver and compromise the immune response of pigs exposed to Fusarium mycotoxins. This discrepancy in results might be attributed to differences in age of pigs, types of grain as mycotoxin sources, and composition of Fusarium mycotoxins in grains as well as volume of blood sample collected (Danicke et al., 2004b).

Exp. 2

A rapid compensation in feed intake and BW gain of the pigs fed the corn-SBM-based standard diet for 14 days was in accordance with Danicke et al. (2004b) who observed quick and complete growth compensatory effects. During the first week of the recovery period, feed intake of pigs previously fed contaminated diets already returned to that of pigs fed diet without contamination and this remained similar following 7 days. In addition, the difference in ADG between the pigs previously fed diets including 0 and 15% or 45% contaminated barley was significant for the period during d 0 to 7 and the overall period, which resulted in the greater G:F in the pigs previously fed diet containing 15% or 45% contaminated barley for the respective periods.

In conclusion, the present study showed that feeding of barley contaminated with Fusarium mycotoxin negatively affects the growth performance of pigs primarily by feed intake depression but these adverse effects were quickly recovered in 7 days after the diets were replaced to the standard corn-SBM diet. The serum immunoglobulin and blood chemical components were not altered by feeding of contaminated barley diets whereas improvement in the digestibility of DM, OM, and CP was observed and further research is needed to verify underlying mechanisms on this improvement.

http://dx.doi.org/10.5713/ajas.14.0707

ACKNOWLEDGMENTS

This paper was supported by the KU Research Professor Program of Konkuk University and Rural Development Administration (Republic of Korea; PJ010932).

REFERENCES

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Submitted Sept. 11, 2014; Revised Oct. 8, 2014; Accepted Dec. 3, 2014

C. Kong, S. Y. Shin, C. S. Park, and B. G Kim *

Department of Animal Science and Technology, Konkuk University, Seoul 143-701, Korea

* Corresponding Author: B. G. Kim. Tel: +82-2-2049-6255, Fax: +82-446-6255, E-mail: bgkim@konkuk.ac.kr
Table 1. Mycotoxin concentrations of ground corn, soybean meal,
and barley as a control and a naturally contaminated one with
Fusarium mycotoxins (as-fed basis), Exp. 1

Mycotoxin         Ground   Soybean         Barley
                   corn     meal

                                     Control   Contaminated

Deoxynivalenol     0.6       1.0       0.3         25.7
  (mg/kg)
Zearalenone         ND       ND        ND          26.0
  ([micro]g/kg)

ND, not detected.

Table 2. Ingredient and nutrient composition (%) of experimental
diets containing increasing levels of barley contaminated with
Fusarium mycotoxins (as-fed basis), Exp. 1

Item                                         Diet (1)

                                     Contaminated barley (%)

                                  0      15      30      45

Ingredient
  Ground corn                   21.8    21.8    21.8    21.8
  Soybean meal (48% CP)         30.6    30.6    30.6    30.6
  Control barley                45.0    30.0    15.0     --
  Contaminated barley            --     15.0    30.0    45.0
  Limestone                     0.95    0.95    0.95    0.95
  Dicalcium phosphate           0.75    0.75    0.75    0.75
  Salt                          0.40    0.40    0.40    0.40
  Vitamin-mineral premix (2)    0.50    0.50    0.50    0.50
Analyzed nutrient and energy
  Dry matter                    89.3    89.1    89.5    89.4
  Organic matter                84.2    83.5    83.0    83.3
  CP                            21.6    22.1    22.8    23.8
  Metabolizable energy          3,131   3,131   3,131   3,131
    (3) (kcal/kg)
Analyzed mycotoxin
  Deoxynivalenol (mg/kg)         0.6     6.1     7.7    14.6
  Zearalenone (pg/kg)            ND     35.4    47.8    33.7

CP, crude protein; ND, not detected.

(1) Each diet contains 0%, 15%, 30%, or 45% contaminated barley
at the expense of control barley, respectively.

(2) Provided the following quantities per kg of complete diet:
vitamin A, 25,000 IU; vitamin D3, 4,000 IU; vitamin E, 50 IU;
vitamin K, 5.0 mg; thiamin, 4.9 mg; riboflavin, 10.0 mg;
pyridoxine, 4.9 mg; vitamin B12, 0. 06 mg; pantothenic acid, 37.5
mg; folic acid, 1.10 mg; niacin, 62 mg; biotin, 0.06 mg; Cu, 25
mg as copper sulfate; Fe, 268 mg as iron sulfate; I, 5.0 mg as
potassium iodate; Mn, 125 mg as manganese sulfate; Se, 0.38 mg as
sodium selenite; Zn, 313 mg as zinc oxide;
butylatedhydroxytoluene, 50 mg.

(3) Calculated value based on NRC (2012).

Table 3. Ingredient and nutrient composition (%) of recovery diet
(as-fed basis), Exp. 2

Item                            Diet

Ingredient
  Ground corn                   67.2
  Soybean meal (48% CP)         28.0
  Soybean oil                   2.00
  L-Lys-HCl (78.8%)             0.20
  Limestone                     0.90
  Dicalcium phosphate           1.00
  Salt                          0.40
  Vitamin premix (1)            0.10
  Mineral premix (2)            0.20
Analyzed nutrient and energy
  Dry matter                    84.5
  CP                            19.1
  Metabolizable energy          3,384
    (3) (kcal/kg)

CP, crude protein.

(1) Provided the following quantities per kg of diet: vitamin A,
20,000 IU; vitamin D3, 4,200 IU; vitamin E, 100 IU; vitamin K3,
5.6 mg; thiamin, 2.8 mg; riboflavin, 5.5 mg; pantothenic acid, 14
mg; pyridoxin, 4.2 mg; biotin, 0.1 mg; cyanocobalamin, 0.042 mg;
niacin, 42 mg; folacin, 1.1 mg; ethoxyquin, 1.1 mg.

(2) Provided the following quantities as milligram per kg of
diet: Fe, 100 mg as iron sulfate; Cu, 70 mg as copper sulfate;
Zn, 48 mg as zinc sulfate; Mn, 60 mg as manganese sulfate; Co,
0.4 mg as cobalt sulfate; I, 0.8 mg as calcium iodate; Se, 0.5 mg
as sodium selenite.

(3) Calculated value based on NRC (2012).

Table 4. Growth performance of pigs fed experimental diets
containing increasing amounts of barley naturally contaminated with
Fusarium mycotoxins (1), Exp. 1

Item                             Diet (2)

                          Contaminated barley (%)

                      0      15      30      45

d 0 to 7
  Initial BW (kg)   32.5    33.0    33.0    34.8
  ADG (g/d)          589     614     475     57
  ADFI (g/d)        1,332   1,297   1,155    848
  G:F               0.42    0.47    0.38    0.06
  Final BW (kg)     36.7    37.3    36.3    35.2
d 7 to 14
  ADG (g/d)          575     511     211     171
  ADFI (g/d)        1,768   1,658   1,281   1,071
  G:F               0.32    0.31    0.13    0.16
  Final BW (kg)     40.7    40.9    37.8    36.4
d 0 to 14
  ADG (g/d)          582     563     343     114
  ADFI (g/d)        1,550   1,478   1,218    959
  G:F               0.37    0.38    0.26    0.11

Item                SEM           p-value

                           Linear    Quadratic

d 0 to 7
  Initial BW (kg)   1.0     0.143      0.497
  ADG (g/d)         122     0.011      0.104
  ADFI (g/d)        163     0.056      0.425
  G:F               0.08    0.011      0.044
  Final BW (kg)     1.3     0.385      0.517
d 7 to 14
  ADG (g/d)          81     0.002      0.881
  ADFI (g/d)         86    < 0.001     0.572
  G:F               0.07    0.063      0.724
  Final BW (kg)     1.2     0.017      0.531
d 0 to 14
  ADG (g/d)          69    < 0.001     0.163
  ADFI (g/d)        114     0.003      0.435
  G:F               0.04   < 0.001     0.087

SEM, standard error of the mean; BW, body weight; ADG average
daily gain; ADFI, average daily feed intake; G:F, gain:feed.

(1) Each least squares mean represents 4 observations.

(2) Each diet contains 0%, 15%, 30%, or 45% contaminated barley
at the expense of control barley, respectively.

Table 5. Vulva size of pigs fed experimental diets containing
increasing amounts of barley naturally contaminated with Fusarium
mycotoxins (1), Exp. 1

Item                                Diet (2)

                             Contaminated barley (%)

                           0      15     30     45

d 0
  Width (mm)              19.7   17.3   19.0   16.5
  Length (mm)             24.7   22.0   19.0   21.5
  Area (3) ([mm.sup.2])   245    193    182    179
d 3
  Width (mm)              20.0   16.3   19.8   17.5
  Length (mm)             27.0   26.3   23.3   26.8
  Area ([mm.sup.2])       284    215    233    235
d 6
  Width (mm)              20.0   17.3   20.0   17.5
  Length (mm)             27.8   28.5   26.8   27.0
  Area ([mm.sup.2])       287    247    269    236
d 9
  Width (mm)              21.5   18.3   20.8   18.0
  Length (mm)             31.0   27.5   25.8   27.5
  Area ([mm.sup.2])       344    251    269    248
d 12
  Width (mm)              20.5   16.5   19.3   18.0
  Length (mm)             30.3   27.8   26.5   27.8
  Area ([mm.sup.2])       319    228    260    250

Item                      SEM         p-value

                                Linear   Quadratic

d 0
  Width (mm)              2.3   0.432      0.990
  Length (mm)             1.8   0.121      0.135
  Area (3) ([mm.sup.2])   36    0.198      0.466
d 3
  Width (mm)              1.9   0.653      0.706
  Length (mm)             1.5   0.601      0.203
  Area ([mm.sup.2])       36    0.439      0.350
d 6
  Width (mm)              1.9   0.589      0.949
  Length (mm)             1.4   0.531      0.860
  Area ([mm.sup.2])       35    0.421      0.922
d 9
  Width (mm)              2.0   0.397      0.904
  Length (mm)             1.9   0.176      0.193
  Area ([mm.sup.2])       43    0.191      0.425
d 12
  Width (mm)              1.7   0.551      0.443
  Length (mm)             1.5   0.229      0.247
  Area ([mm.sup.2])       37    0.314      0.308

SEM, standard error of the mean.

(1) Each least squares mean represents 4 observations except the
diet containing 0% contaminated barley in d 0 (3 observations).

(2) Each diet contains 0, 15, 30, and 45% contaminated barley at
the expense of control barley, respectively.

(3) Area ([mm.sup.2]) = [Width (mm) x Length (mm)]/2.

Table 6. Dry matter (DM), organic matter (OM), and crude protein
(CP) digestibility of pigs fed experimental diets containing
increasing amounts of barley naturally contaminated with Fusarium
mycotoxins (1), Exp. 1

Item                             Diet (2)           SEM

                           Contaminated barley (%)

                         0      15     30     45

DM intake (kg/d)        1.66   1.57   1.17   1.03   0.09
OM intake (kg/d)        1.57   1.47   1.08   0.96   0.08
CP intake (g/d)         404    388    297    274     22
Fecal DM output (g/d)   322    294    205    168     29
Fecal OM output (g/d)   281    255    174    142     26
Fecal CP output (g/d)   82.1   72.5   49.9   34.4   9.1
DM digestibility (%)    80.4   81.3   83.0   83.8   1.2
OM digestibility (%)    81.8   82.7   84.5   85.3   1.2
CP digestibility (%)    79.3   81.4   84.4   87.6   1.8

Item                            p-value

                        Linear    Quadratic

DM intake (kg/d)         0.001      0.820
OM intake (kg/d)        < 0.001     0.882
CP intake (g/d)          0.002      0.881
Fecal DM output (g/d)    0.004      0.890
Fecal OM output (g/d)    0.004      0.908
Fecal CP output (g/d)    0.005      0.757
DM digestibility (%)     0.065      0.954
OM digestibility (%)     0.060      0.969
CP digestibility (%)     0.011      0.756

SEM, standard error of the mean.

(1) Each least squares mean represents 4 observations except the
diet containing 0% contaminated barley (3 observations).

(2) Each diet contains 0%, 15%, 30%, or 45% contaminated barley
at the expense of control barley, respectively.

Table 7. Immunoglobulin and biological blood assay of pigs fed
experimental diets containing increasing amounts of barley
naturally contaminated with Fusarium mycotoxins (1,2), Exp. 1

Item                                   Diet (3)

                                Contaminated barley (%)  SEM

                              0      15     30     45

Immunoglobulin (mg/dL)
  IgA                        46.0   46.5   44.8   43.3   2.6
  IgG                        661    662    662    745     46
  IgM                        46.3   72.5   60.3   85.3   11.6
Biological blood assay (5)
  Albumin (g/dL)             3.98   4.00   3.43   3.68   0.22
  Globulin (g/dL)            2.50   2.68   3.33   3.20   0.29
  Albumin:globulin           1.82   1.50   1.10   1.17   0.28
  Total protein (g/dL)       6.48   6.68   6.75   6.88   0.26
  ALP (U/L)                  206    117    102     99     40
  ALT (U/L)                  38.3   44.3   43.5   52.0   4.3
  AST (U/L)                  90.0   67.8   39.5   79.3   31.9
  GGT, U/L                   65.8   55.8   54.5   43.5   11.8
  BUN (mg/dL)                15.9   19.3   17.4   21.6   1.2
  Calcium (mg/dL)            10.7   11.2   10.4   10.6   0.3
  Glucose (mg/dL)            81.8   69.8   81.3   74.3   3.6
  Phosphorus (mg/dL)         10.1   11.1   9.2    9.6    0.4

Item                               p-value

                             Linear   Quadratic

Immunoglobulin (mg/dL)
  IgA                        0.412      0.709
  IgG                        0.254      0.399
  IgM                        0.075      0.958
Biological blood assay (5)
  Albumin (g/dL)             0.161      0.615
  Globulin (g/dL)            0.062      0.616
  Albumin:globulin           0.090      0.493
  Total protein (g/dL)       0.296      0.887
  ALP (U/L)                  0.094      0.304
  ALT (U/L)                  0.064      0.777
  AST (U/L)                  0.682      0.357
  GGT, U/L                   0.231      0.967
  BUN (mg/dL)                0.016      0.737
  Calcium (mg/dL)            0.449      0.755
  Glucose (mg/dL)            0.516      0.509
  Phosphorus (mg/dL)         0.136      0.463

SEM, standard error of the mean; ALP, alkaline phosphatase; ALT,
Ala transaminase; AST, Asp transaminase; BUN, blood urea
nitrogen; GGT, [gamma]-glutamyl transferase.

(1) Each least squares mean represents 4 observations.

(2) Blood samples were collected at the end of the 14-d trial.

(3) Each diet contains 0%, 15%, 30%, or 45% contaminated barley
at the expense of control barley, respectively.

Table 8. Growth performance of pigs fed the recovery diet (1),
Exp. 2

Item                         Diet (2)                  SEM    p-value

                           Contaminated barley (%)

                      0       15       30       45

d 0 to 7
  Initial BW (kg)    46.8    46.3     42.8     41.6    1.5     0.085
  ADG (g/d)          614     1,032    589     1,086     86     0.004
  ADFI (g/d)        1,976    2,281   1,804    2,110    117     0.090
  G:F                0.31    0.44     0.33     0.51    0.03    0.005
  Final BW (kg)      51.1    53.6     46.9     49.2    1.8     0.136
d 7 to 14
  ADG (g/d)         1,2 89   1,389   1,236    1,461     86     0.313
  ADFI (g/d)        2,594    2,474   2,184    2,781    191     0.233
  G:F                0.50    0.60     0.56     0.53    0.06    0.629
  Final BW (kg)      60.1    63.3     55.5     59.4    2.2     0.174
d 0 to 14
  ADG (g/d)          952     12 11    913      1273     75     0.016
  ADFI (g/d)        2,285    2,378   1,994    2,445    132     0.148
  G:F                0.42    0.52     0.46     0.52    0.03    0.147

Item                   p-values for contrast (%)

                    0 vs 15   0 vs 30   0 vs 45

d 0 to 7
  Initial BW (kg)    0.826     0.085     0.035
  ADG (g/d)          0.008     0.842     0.004
  ADFI (g/d)         0.099     0.329     0.441
  G:F                0.017     0.644     0.002
  Final BW (kg)      0.369     0.137     0.482
d 7 to 14
  ADG (g/d)          0.432     0.670     0.192
  ADFI (g/d)         0.668     0.164     0.506
  G:F                0.233     0.467     0.728
  Final BW (kg)      0.340     0.175     0.828
d 0 to 14
  ADG (g/d)          0.036     0.718     0.014
  ADFI (g/d)         0.630     0.153     0.411
  G:F                0.059     0.421     0.056

SEM, standard error of the mean; BW, body weight; ADG average
daily gain; ADFI, average daily feed intake; G:F, gain:feed.

(1) Each least squares mean represents 4 observations.

(2) Each of previously fed diets contained 0%, 15%, 30%, or 45%
contaminated barley at the expense of control barley,
respectively.

Table 9. Vulva size of pigs fed the recovery diet (1), Exp. 2

Item                             Diet (2)

                            Contaminated barley (%)   SEM   p-value

                           0      15     30     45

d 0
  Width (mm)              19.8   16.5   20.3   16.8   2.1    0.485
  Length (mm)             29.3   28.3   27.5   26.8   1.6    0.721
  Area (3) ([mm.sup.2])   610    471    567    448    80     0.468
d 3
  Width (mm)              27.0   26.8   28.5   28.0   2.0    0.914
  Length (mm)             31.0   31.0   31.3   32.8   1.0    0.538
  Area ([mm.sup.2])       840    831    892    924    88     0.856
d 6
  Width (mm)              27.5   26.8   27.8   29.5   1.8    0.735
  Length (mm)             33.8   32.0   30.8   33.0   1.1    0.300
  Area ([mm.sup.2])       931    857    854    974    77     0.639
d 9
  Width (mm)              27.3   28.3   29.5   29.8   1.0    0.325
  Length (mm)             33.5   31.3   31.5   33.3   0.7    0.111
  Area ([mm.sup.2])       915    884    929    989    42     0.401
d 12
  Width (mm)              27.3   27.0   29.5   30.5   1.1    0.139
  Length (mm)             33.0   30.5   32.0   32.3   0.8    0.218
  Area ([mm.sup.2])       900    824    947    984    50     0.193

Item                         p-values for contrast (%)

                          0 vs 15   0 vs 30   0 vs 45

d 0
  Width (mm)               0.299     0.869     0.336
  Length (mm)              0.666     0.455     0.294
  Area (3) ([mm.sup.2])    0.252     0.714     0.187
d 3
  Width (mm)               0.932     0.610     0.733
  Length (mm)              1.000     0.858     0.228
  Area ([mm.sup.2])        0.944     0.685     0.512
d 6
  Width (mm)               0.771     0.923     0.445
  Length (mm)              0.286     0.083     0.638
  Area ([mm.sup.2])        0.515     0.496     0.698
d 9
  Width (mm)               0.501     0.149     0.113
  Length (mm)              0.052     0.078     0.810
  Area ([mm.sup.2])        0.614     0.816     0.246
d 12
  Width (mm)               0.877     0.187     0.069
  Length (mm)              0.050     0.390     0.515
  Area ([mm.sup.2])        0.305     0.517     0.261

SEM, standard error of the mean.

(1) Each least squares mean represents 4 observations.

(2) Each of previously fed diets contained 0%, 15%, 30%, or 45%
contaminated barley at the expense of control barley,
respectively.

(3) Area ([mm.sup.2]) = [Width (mm)xLength (mm)]/2.
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Article Details
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Author:Kong, C.; Shin, S.Y.; Park, C.S.; Kim, B.G.
Publication:Asian - Australasian Journal of Animal Sciences
Article Type:Report
Geographic Code:9SOUT
Date:May 1, 2015
Words:6996
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