Effects of anterior chamber depth and axial length on corneal endothelial cell density after phacoemulsification.
KEYWORDS: Phacoemulsification, Corneal Endothelial Cell Density, Anterior Chamber Depth, Axial Length.
Corneal endothelial cells subserve a very important role of controlling corneal hydration by their pump mechanism, apart from other functions. Their loss below critical number (45 sec)
Before surgery, all subjects underwent complete ophthalmic examination including visual acuity measurement and slit lamp examination. Further evaluation included automated refraction using Canon RK-F1 Full Auto Ref-Keratometer, three measurements of CCT and CED using Topcon SP 3000P Specular Microscope (Topcon Corporation, Tokyo, Japan) and measurement of ALs and ACDs using IOL MasterA(r).
Eyes were divided into two groups according to ACD and AL: Group-I: ACD 2.0mm - 3.0mm and AL 22mm - 23.5mm; Group-II: ACD 3.1 mm -4.0 mm and AL 23.6mm - 25mm. All cataracts were graded according to Lens Opacities Classification System III (LOCS III) and only those with Nuclear Opalescence 2 and 3 (NO-2 and NO-3) were included.
After preoperative recordings, the subjects underwent successful phacoemulsification surgery and posterior chamber IOL implantation. Phacoemulsification with IOL implantation was performed in all patients by same surgeon using same phaco machine (Oertli, Faros, Switzerland) and operation room set up under local anaesthesia (peribulbar) with 2% lignocaine. In all cases combination of 1% sodium hyaluronate (Provisc, Alcon) and 2% hydroxypropyl methylcellulose (Viscot, Alcon) was used as viscoelstics. Phaco power and phaco time were recorded for each patient.
All patients were re-examined two month postoperatively. Again three readings of CED and CCT were obtained.All data was recorded using pre-devised proforma for record keeping. Statistical package for social sciences (SPSS 22.0) for windows was used for statistical analysis. Descriptive statistics i.e. mean +- standard deviation for quantitative values (age, CCT, ACD, AL, CED) and frequencies along with percentages for qualitative variables (gender, laterality) were used to describe the data. Shapiro Wilk's test was used to check normality of data. Post normality testing, Chi square test was used to compare qualitative variables, and independent t test to compare quantitative variables between two groups. Moreover, Paired 't' test was used to compare postoperative value from pre-operative value within each group. P-value < 0.05 was considered statistically significant.
Table-I: Group wise demographic data (n=100).
Age(Years)###62.90+- 7.17###62.68+-7.03###63.12+- 7.32###0.726**
Mean +- SD
Mean +- SD
Mean +- SD
Mean +- SD
Mean Change in CED(Cells/mm2)###213.08+-199.44###259.38+-116.78###166.7840+-248.44###0.019**
Mean +- SD
Mean Frequency Change in CED(%)###7.61+-6.59###9.30+-3.99###5.9197+-8.07###0.010**
Mean +- SD
Table-II: Pre-op and post-op comparison between two groups.
###CED (Cells/mm2) mean +- SD
Mean age, gender distribution, laterality of eyes, AL, ACD, pre-operative CED, post-operative CED, mean change in CED and mean frequency change in CED of study population, and of both groups is given in Table-I. The difference in laterality, age and preoperative CED, and post-operative CED between two groups was not statistically significant. However, difference in mean change in CED and mean frequency change in CED between two groups was found to be statistically significant (p = <0.05). The comparison of pre-operative CED from post-operative CED within each group is given in Table-II. Difference of pre-operative CED from post-operative CED in both groups was statistically significant.
Corneal endothelial cells are key indicators of corneal integrity and function. They constantly dehydrate the cornea and also form the descemet membrane. Their significance in healthy as well as diseased has been emphasized greatly during last few decades after invent of reliable machines for analyzing endothelial cell morphology and function. A specular microscope is a reflected light microscope that works by projecting light onto the cornea and then imaging the reflected light from optical interface between corneal endothelium and aqueous humor. Those images are analyzed by the machine and give us different endothelial cell parameters like number, density, variation in size and hexagonality. In our study we have analyzed the endothelial cell number loss only, among other endothelial cell parameters.
Corneal endothelial cell loss (CED loss) leads to loss of hexagonality of surrounding cells intended to fill the space.6 With greater loss, the pump mechanism of remaining cells is insufficient to dehydrate cornea and edema ensures (compromised cornea); leading to decreased corneal transparency. This greatly affects the visual acuity of the patient, which eventually becomes a permanent loss.
CED loss during phacoemulsification is unavoidable. It is mainly due to very small confined space (Anterior Chamber) where surgery is performed. Corneal endothelium comes in direct contact with instruments multiple times during surgery making them vulnerable to damage by high ultrasound energy of phaco tip. Hence it is presumed that shallow anterior chamber is a risk factor for CED loss preoperatively and deeper anterior chamber is safer comparatively.
CED loss has been studied with respect to various modifiable and non modifiable factors. We have conducted our study with focus on anatomical non modifiable factor such as AL and ACD. We used IOL MasterA(r) for determination of AL and ACD. Other non modifiable risk factors for CED loss include diabetes mellitus, age and cataract density. The modifiable risk factors for CED loss include corneal incision size, phacoemulsification time, mean ultrasound power, irrigation solution turbulence, instrument-related trauma, type of viscoelastic substances being used, nuclear fragments and IOL contact during insertion and the type of implanted IOL.6-8
Cataract density is a non modifiable risk factor for CED loss. As can be expected, dense cataracts require more energy and time to break with greater manipulation within the limited chamber; making corneal endothelium vulnerable to damage. We selected cases with moderate cataract densities (NO-2 and NO-3) in our study to avoid major differences in surgical handling and techniques. Moreover, same phaco machine was used and same surgeon performed all surgeries with same surgical technique. This plays down chances of partiality in results.
A number of studies have precluded that AL and ACD influence the CED loss.11,12 Others have turned down any effect of these parameters on endothelial damage.9,10 Cho et al. measured the decrease in CED in different anterior chamber depth groups, with no significant difference of CED loss within these groups.13 In the study by Hwang BH et al., CED loss was higher in smaller ACD group as compared to large ACD groups, but the results were not statistically significant.14 In present study we divided the cases undergoing phacoemulsification into two groups according to ACD and AL: Group-I: ACD 2.0mm - 3.0mm and AL 22mm - 23.5mm; Group-II: ACD 3.1 mm -4.0 mm and AL 23.6mm - 25mm. We documented statistically significant effect of shallow anterior chamber and smaller AL on CED loss (p = <0.05).This is the first study in Pakistan that has been conducted on specific parameters and their influence on one parameter i.e. CED loss.
We hope this study will help phaco surgeons in planning surgeries in eyes with shallow anterior chambers with greater care and better techniques. Moreover, this will open doors for research on better and safer techniques in shallow anterior chambers and small eyes.
Conflict of interests: The authors report no conflict of interests. The authors alone are responsible for the content and writing of the paper. None of the authors has a financial or proprietary interest in any material or method mentioned.
Grant Support and Financial Disclosures: None.
1. Bowling B. Cornea. 'Kanski's Clinical Ophthalmology: A Systematic Approach' (eighth edition). Saunders Ltd. 2016:6:168.
2. Kohlhaas M, Stahlhut O, Tholuck J, Richard G. Changes in corneal thickness and endothelial cell density after cataract extraction using phacoemulsification. Ophthalmologe 1997;94(7):515-518. doi: 10.1007/s003470050150.
3. Jorge J, Queiros A, Peixoto-de-Matos SC, Ferrer-Blasco T, Gonzalez-Meijome JM. Age-related changes of corneal endothelium in normal eyes with a non-contact specular microscope. J Emmetropia. 2010;1(2):132-139.
4. Moller-Pedersen T. A comparative study of human corneal keratocyte and endothelial cell density during aging. Cornea. 1997;16(3):333-338.
5. Niederer RL, Perumal D, Sherwin T, McGhee CN. Age-related differences in the normal human cornea: a laser scanning in vivo confocal microscopy study. Brit J Ophthalmol. 2007;91(9):1165-1169. doi: 10.1136/bjo.2006.112656.
6. Waring GO III, Bourne WM, Edelhauser HF, Kenyon KR. The corneal endothelium. Normal and pathologic structure and function. Ophthalmol. 1982;89(6):531-590. doi: 10.1016/S0161-6420(82)34746-6.
7. Storr-Paulsen A, Norregaard JC, Ahmed S, Storr-Paulsen T, Pedersen TH. Endothelial cell damage after cataract surgery: divide-and-conquer versus phaco-chop technique. J Cataract Refract Surg. 2008;34(6):996-1000. doi: 10.1016/j. jcrs.2008.02.013.
8. Holzer MP, Tetz MR, Auffarth GU, Welt R, Volcker HE. Effect of Healon 5, 4 and other viscoelastic substances on intraocular pressure and endothelium after cataract surgery. J Cataract Refract Surg. 2001;27(2):213-218. doi: 10.1016/S0886-3350(00)00568-X.
9. Reuschel A, Bogatsch H, Oertel N, Wiedemann R. Influence of anterior chamber depth, anterior chamber volume, axial length, and lens density on postoperative endothelial cell loss. Graefe's Arch Clinic Experim Ophthalmol. 2015;253(5):745-752. doi: 10.1007/s00417-015-2934-1.
10. Walkow T, Anders N, Klebe S. Endothelial cell loss after phacoemulsification: relation to preoperative and intraoperative parameters. J Cataract Refract Surg. 2000;26(5);727-732. doi: 10.1016/S0886-3350(99)00462-9.
11. Hugod M, Storr-Paulsen A, Norregaard JC, Nicolini J, Larsen AB, Thulesen J. Corneal endothelial cell changes associated with cataract surgery in patients with type 2 diabetes mellitus. Cornea. 2011;30(7):749-753. doi: 10.1097/ICO.0b013e31820142d9.
12. Yamazoe K, Yamaguchi T, Hotta K, Satake Y, Konomi K, Den S, Shimazaki J. Outcomes of cataract surgery in eyes with a low corneal endothelial cell density. J Cataract Refract Surg. 2011;37(12):2130-2136. doi: 10.1016/j.jcrs.2011.05.039.
13. Cho YK, Chang HS, Kim MS. Risk factors for endothelial cell loss after phacoemulsification: comparison in different anterior chamber depth groups. Korean J Ophthalmol. 2010;24(1):10-15. doi: 10.3341/kjo.2010.24.1.10.
14. Hwang HB, Lyu B, Yim HB, Lee NY. Endothelial Cell Loss after Phacoemulsification according to Different Anterior Chamber Depths. J Ophthalmol. 2015;2015:210716. doi: 10.1155/2015/210716.
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|Publication:||Pakistan Journal of Medical Sciences|
|Date:||Feb 28, 2019|
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