Effects of acute leg ischemia during cycling on oxygen and carbon dioxide stores.
Progressive physical deconditioning is common in patients with chronic diseases, such as congestive heart failure and chronic obstructive pulmonary disease. One limitation these patients face is an inability to exercise with sufficient intensity to provide adequate training stimuli. However, regional training of muscles without taxing the central circulation can improve whole-body exercise capacity in these patients . An unusual potential tool to facilitate regional muscle rehabilitation is exercise training during reduced limb blood flow [2-3]. Such "ischemic limb training" with limb pressure cuffs has improved limb strength and exercise endurance in physically fit subjects [4-5], diminished postoperative disuse atrophy of knee extensors , and induced favorable biochemical and structural changes in muscles [7-8]. Ischemic limb training with low-intensity exercise in patients with congestive heart failure has also reduced exertional dyspnea . We recently demonstrated that leg-extension exercise endurance was enhanced with a 6-week training program of very light leg-extension exercise with ischemia induced by thigh cuff inflation .
Superimposing ischemia on exercising limbs provokes the muscle metaboreflex, whereby pulmonary ventilation ([[??].sub.E]) and systemic blood pressure are elevated by a chemoreflex stimulated by buildup of metabolic byproducts in the ischemic limbs; the most likely candidate is hydrogen ion concentration ([H.sup.+]) . The oxygen ([O.sub.2]) stores ([O.sub.2]s) and carbon dioxide ([CO.sub.2]) stores ([CO.sub.2]s) in the region where blood flow is occluded, as well as in the whole body, will be affected during this ischemia and after circulation is restored as a result of ventilatory, blood flow, and biochemical perturbations. The magnitude and time course of these gas store changes will affect regional and whole-body acid-base status, will cause secondary ventilatory and gas exchange fluctuations during and after exercise, and may induce transient hypoxemia and hypercapnia, such as noted following passive changes in posture .
Although rapid transient changes in [O.sub.2]s and [CO.sub.2]s during exercise workload transitions have been studied and quantified , gas store changes induced by limb ischemia have received little attention. Specifically, the quantitative relationship is not well defined between [O.sub.2] repayment and [CO.sub.2] elimination after exercise requiring energy partially derived from anaerobic sources  and these measurements with the anaerobic component artificially superimposed have not been reported. Therefore, this study was an initial attempt to estimate the time course and magnitude of changes in [O.sub.2]s and [CO.sub.2]s during and after acute, temporary ischemia of the legs applied by cuff inflation during steady state exercise on a cycle ergometer.
Five men and one woman volunteered as subjects. Informed consent was obtained from each person, as approved by the University of New Mexico Human Research Review Committee. All were physically fit and regularly taking part in physical recreation and fitness activities, including jogging and cycling. Their ages ranged from 24 to 62 yr, with a mean body weight and body mass index of 82.5 kg and 25.0 kg/[m.sup.2], respectively. Their maximal [O.sub.2] uptake ([??][O.sub.2]max) averaged 48 [mL.min.sup.-1].[kg.sup.-1] (range: 42-56). The [O.sub.2] uptake ([??][O.sub.2]) during exercise before ischemia (baseline) averaged 35.7 percent (range: 30%-42%, standard error of the mean = 1.7%) of the subjects' [??][0.sub.2max]. This percentage was not related to age (r = -0.22).
Ergometer Exercise and Inflation Cuffs
We placed cuffs on each upper thigh (SC-17, Hokanson Co; Bellevue, Washington) and each lower leg (SC22) using adhesive tape to keep them in position during exercise. Lower leg cuffs were used to minimize trapping of blood and to enhance ischemia of the calf muscles. Cuffs were inflated to 140 mmHg during exercise. This cuff pressure, slightly exceeding systolic pressure, was chosen after preliminary trials indicated that discomfort at this pressure could be tolerated and gas exchange transients stabilized in about 5 min at the chosen workload. Although the blood pressure response of each subject to the inflation pressure varied, we maintained the pressure at the same level for all to reduce variations in blood "pooling" and thereby reduce variability in the measured responses. Resting measurements were made for 5 min while subjects sat on the ergometer before and after exercise. Subjects cycled for 15 min at 75 W on an electrically load-controlled Bosch ergometer (model ERG 551; Munich, Germany) at 50 to 60 rpm. After 5 min, the four cuffs were simultaneously inflated over a [approximately equal to]10 s period from a gas cylinder pressure source. Cuff pressure was maintained for 5 min and then deflated rapidly in 3 s, with exercise continuing for another 5 min.
Measurements and Calculations
We measured gas exchange at the mouth while subjects sat on the ergometer at rest, during exercise, and at rest after exercise, using a TrueMax 2400 breath-by-breath automated system (Parvomedics, Inc; Sandy, Utah) with incorporated software and model 2700 Rudolph breathing valve and mouthpiece (Hans Rudolph, Inc; Shawnee, Kansas). The measurements included [??][O.sub.2], [CO.sub.2] Output ([??][CO.sub.2]), [[??].sub.E], calculated respiratory exchange ratio (RER), and [[??].sub.E]/ V [CO.sub.2] as an index of ventilatory drive. Alveolar ventilation ([[??].sub.A]) was calculated from anatomical dead space (ADS) taken as apparatus dead space + milliliter = body weight in pounds  and breathing frequency (f) as [??]A = [[??].sub.E] - f X ADS. Experiments were conducted at an average barometric pressure ([P.sub.B]) of 631 mmHg (range: 630-635 mmHg) and ambient fraction of inspired [O.sub.2] (17,[O.sub.2]) of 0.2094. Partial pressure of [CO.sub.2] (P[CO.sub.2]) in alveoli (PA[CO.sub.2]) and partial pressure of [O.sub.2] (P[O.sub.2]) in alveoli (PA[O.sub.2]) were calculated from alveolar gas equations : PA[CO.sub.2] = ([??][CO.sub.2] X 0.863)ivA (1)
'A[O.sub.2] = ([P.sub.B] [??] 47. 1)FI[O.sub.2] [??] PA[CO.sub.2] [FI[O.sub.2] + (1 [??] FI[O.sub.2])/RER]. (2;
We averaged breath-by-breath measurements continuously over 30 s intervals for each subject throughout exercise and the pre- and postexercise rest periods. We then averaged these values for the six subjects to obtain representative temporal patterns for analysis.
Average changes in [O.sub.2]s and [CO.sub.2]s were calculated from differences between measured and predicted gas exchange time courses integrated over time. We based pre dicted values on baseline gas exchange measurements during the 5th min, assuming these represented steady state values required by the workload, and an increase during ischemia based on assumptions given in the subsequent section for predicted gas exchange. An increase in [O.sub.2]s was indicated when measured [??][O.sub.2] is greater than predicted [??][O.sub.2] over time, and a decrease in [CO.sub.2]s was indicated when measured [??] [CO.sub.2] is greater than predicted [??] [CO.sub.2] and vice versa. Differences in these gas store changes during and after blood flow restriction were attributed to the ischemia. In addition, we obtained total body gas stores present during baseline, 5th min during cuff inflation, and 5th min after cuff deflation from a model using gas exchange, blood flow, and blood volume values. We also used differences between these modeled total store values and the time-integrated measured values of changes in [O.sub.2]s and [CO.sub.2]s to extract effects of leg ischemia.
Predicted Gas Exchange
During cuff inflation, we assumed the predicted time course for [??][O.sub.2] would increase linearly during the 6th through 10th min from the steady state exercise value at 5 min because of--
1. A gradual loss of mechanical efficiency by increasing recruitment of ancillary muscles of the hip, torso, and arms to maintain leg work as fatigue increased.
2. Increased [O.sub.2] cost of ventilation stimulated by the metaboreflex, which may account for as much as one-third of the observed [??][O.sub.2] rise [17-18].
3. The partial restoration of curtailed leg circulation by the reflex rise in blood pressure that would enhance [O.sub.2] delivery to the legs despite restricted blood flow during cuff inflation.
4. The subjects' subjective reports that the last minute of exercise seemed less stressful than the previous minutes, indicating that the anaerobic component of the energy supply had stabilized.
During the 5 min following cuff deflation, [??][O.sub.2] was assumed to decline exponentially to the baseline exercise value by 15 min because the factors just listed were removed by cuff deflation and the elevated [??][O.sub.2] was expected to return similarly to that following the removal of an additional acute exercise workload. The predicted [??][CO.sub.2] was similarly assumed to increase linearly from baseline to 10 min, but to a value calculated as measured [??][O.sub.2] X measured baseline RER before cuff inflation (for correcting the elevated [??][CO.sub.2] from the increase in [[??].sub.E] resulting from the metaboreflex), and then decline exponentially to the baseline value by 15 min.
Total Gas Stores Model with Blood Flow and Volume Redistribution
Computations and assumptions are shown in the following list for compartmental and total whole body [O.sub.2]s and [CO.sub.2]s during exercise at three exercise conditions A, B, and C: A = baseline, 5th min before cuff inflation; B = 5th min of cuff inflation; and C = 5th min after cuff deflation. Arterial and mixed venous blood [O.sub.2] and [CO.sub.2] contents and mixed venous P[O.sub.2] and P[CO.sub.2] were calculated from a computer model integrating gas exchange and blood flow values [19-20].
* Blood volume.
--Total = 71.5 mL/kg body weight = 5,900 mL.
--Venous compartment for exercise conditions A and C = total X 0.8 = 4,720 mL.
--Arterial compartment for exercise conditions A and C = total X 0.2 = 1,180 mL.
--During condition B, a 300 ml, blood volume shift from the venous to arterial compartment was predicted based on transient increases in measured [??][O.sub.2] and a [??][CO.sub.2] from 30 to 60 s after cuff deflation.
* Lung: [O.sub.2] and [CO.sub.2] were calculated from PA[O.sub.2] and PA[CO.sub.2] and an assumed functional residual capacity of 4.0 L.
* Arterial [O.sub.2]: Content based on Hb (hemoglobin concentration) = 15 g%, arterial P[O.sub.2] = PA[O.sub.2], saturation = standard dissociation curve  at pHa (arterial pH, the negative log of [H.sup.+] in arterial blood) calculated to maintain whole blood base excess (BE) equal to baseline , where a pHa value of 7.420 was assumed.
* Venous [O.sub.2]: Content from Fick equation with arterial content and measured [??][O.sub.2] at, exercise conditions A, B, and C and cardiac output (Q) = 15 L/min at conditions A and C, with 1 L/min reduction during condition B, based on observations during cuff induced ischemia by Asmussen and Nielsen .
* Tissue [O.sub.2].
--P[O.sub.2] from venous content and saturation from standard curve.
--P[O.sub.2] X body weight (82.5 kg) X 0.64 X 0.[O.sub.2]4 .
* Arterial [CO.sub.2].
--Content based on arterial P[CO.sub.2] = PA[CO.sub.2].
--Content from [CO.sub.2] dissociation curve at Hb and pHa .
* Venous [CO.sub.2]: Content from Fick equation with arterial [CO.sub.2] content and measured [??][CO.sub.2] and predicted Q at exercise conditions A, B, and C.
* Tissue [CO.sub.2].
--P[CO.sub.2] for venous content from [CO.sub.2] dissociation curve.
--P[CO.sub.2] X body weight X 1.[O.sub.2].
We obtained half-times for rest-to-exercise ("on") responses and ("off') transitions from exponential fits to the 10 measured breath-by-breath intervals. We used paired Mests to determine significance (p < 0.05) of selected individual transient changes over time and used least squares linear regressions to estimate the significance of relationships between selected variables.
The average [??][O.sub.2] and V [CO.sub.2] measurements during rest, exercise, and postexercise rest are shown in Figure 1. A plateau for both measurements was reached after [approximately equal to] 3 min of exercise, because the 5th min values were not significantly above the 3 min values (p > 0.13). Transient changes induced by ischemia and cuff deflation appeared to have stabilized by the end of exercise. The baseline mechanical efficiency at 75 W for a [??][O.sub.2] of 1,410 mL/min (minus the resting [??][O.sub.2] of 335 mL/min) was 20.0 percent, decreasing to 17.1 percent at 1,595 mL/min by the end of inflation. During the 5 min postexercise rest period, the total excess [??][O.sub.2] and VC [O.sub.2] were both significantly larger than the 5 min [??][O.sub.2] deficits following exercise onset. The averages of the corresponding changes in gas stores calculated from time-integrated values for measured and predicted [??][O.sub.2] and [??][CO.sub.2] are detailed in Figure 2.
[FIGURE 1 OMITTED]
Measured [??][O.sub.2] increased significantly during the first 30 s after cuffs were inflated (p = 0.042) and then declined transiently, but significantly, at 6.5 min by 72 mL/min (p = 0.049). [??][O.sub.2] then rose steadily until cuffs were deflated. The [O.sub.2]s cumulative loss over 5 min of cuff inflation was 227 mL (Figure 2). [??][O.sub.2] peaked 45 s after cuff deflation, being 150 mL above adjacent measurements (p = 0.001). The 5 min postdeflation exercise [??][O.sub.2] excess indicated that [O.sub.2]s increased by 518 mL.
Measured [??][CO.sub.2] during ischemia is related to similar circulatory and biochemical events affecting [??][O.sub.2] but is partially overridden by the large increase in [[??].sub.E] (Figure 3), because of the metaboreflex stimulation by leg ischemia. [CO.sub.2]s decreased by 497 mL by the end of the 5 min inflation, as indicated in Figure 2. Similar to [??][O.sub.2], the [??][CO.sub.2] peaked 45 s after cuff deflation, indicating an additional 180 mL loss in [CO.sub.2]s above the adjacent measurements (p = 0.0[O.sub.2]), corresponding to the 150 mL of [O.sub.2] taken up. The loss in [CO.sub.2]s over 5 min after cuff deflation was 1,162 mL, about double that of the [O.sub.2]s gain (518 mL). Over the 10 min of exercise during cuff inflation and deflation, the total [O.sub.2]s gain was -227 + 518 = 291 mL and the total [CO.sub.2]s loss was 497 + 1,162 = 1,659 mL.
[FIGURE 2 OMITTED]
After exercise termination, the off responses for [??][CO.sub.2] and [[??].sub.E] (Figure 3) were similar to each other and their on-responses (36-39 s) but slower than the on-response for [??][O.sub.2]. [??][O.sub.2] and [??] [CO.sub.2] were slightly above baseline at the end of the 5 min postexercise rest period (Figure 1). The RER was significantly higher during the 5th min postexercise rest compared with the preexercise rest because [??] [CO.sub.2] was significantly higher (30%) than [??][O.sub.2] (18%), indicating a residual enhanced ventilatory drive.
[FIGURE 3 OMITTED]
Whole Body [CO.sub.2]s
By superimposing controlled hyperventilation, one can obtain estimates of whole-body [CO.sub.2]s during exercise. From measurements in these "hyperventilation" experiments dur ing ischemic exercise, the whole-body [CO.sub.2] capacitance (dissociation curve) was 1.2 LxmmHg-lxKg-1], as calculated from the excess of measured vs predicted [??] [CO.sub.2] (497 mL) (Figure 2) per change in PA[CO.sub.2] (5 mmHg) (Figure 3) per body weight (82.5 kg).
Model of Total and Changing Gas Stores
Table 1 shows the compartmental and total gas stores calculated for the three exercise conditions from the flow and volume redistribution model. Because lactate, bicar bonate concentration (HC[O.sub.3.sup.-]), and BE changes are linearly related , we incorporated a decrease in whole blood BE of 4 mmol/L estimated from other studies (see "Discussion") during the 5th min after cuff deflation to account for circulating lactate. The values from the total stores model from Table 1 are indicated in Figure 4 in relation to the 5 min-integrated stores changes obtained from measured gas exchange (Figure 2). According to the model, during cuff inflation, total [O.sub.2]s did not change and [CO.sub.2]s decreased 164 mL, whereas the 5 min totals (Figure 2) decreased 227 and 497 mL, respectively. The difference indicates that the redistribution of blood volume and flow, the anaerobic work component, and hyperventilation resulted in losses of 227 mL and 333 mL in [O.sub.2]s and [CO.sub.2]s, respectively. During the 5th min after cuff deflation, [O.sub.2]s increased by 18 mL and [CO.sub.2]s decreased another 465 mL, whereas the 5 min totals showed that [O.sub.2]s increased by 518 mL and [CO.sub.2]s decreased by 1,162 mL. For [O.sub.2]s, reducing the 518 mL gain after cuff deflation by the 18 mL increase in total stores, as well as the 227 mL deficit during prior inflation (which is being repaid), leaves a net gain of 273 mL used to repay the anaerobic cost during ischemia. The 1,162 mL 5 min loss in [CO.sub.2]s after cuff deflation exceeds the 465 mL loss in absolute stores by 697 mL (Figure 4). Over the total 10 min, 5 min before and 5 min after inflation, the ratio of the total loss in [CO.sub.2]s versus gain in [O.sub.2]s is 3.7 (1,030/ 273), which includes the hyperventilation "artifact" during ischemia.
[FIGURE 4 OMITTED]
The initial increase in [??][O.sub.2] during the 1st min of ischemia can be accounted for by the bolus of venous blood from the legs moving into the central circulation during cuff inflation and its oxygenation to arterial blood as it traverses the pulmonary capillaries. This [approximately equal to]30 mL of [O.sub.2] (Figure 2) would reoxygenate 300 mL of venous blood having an [O.sub.2] content of 10 vol%. This rise in [??][O.sub.2] and the [approximately equal to]30 mL significant simultaneous loss of [CO.sub.2] (p = 0.004) indicated a 300 mL shift of blood from the venous to arterial compartment. A redistribution of blood flow accounted for the transient reduction in [??][O.sub.2] during the 2nd min of ischemia, whereby cuffs restricted [O.sub.2] delivery to the legs by arterial blood, reducing [??][O.sub.2] temporarily and increasing [O.sub.2] content of mixed venous blood. Similar cardiovascular readjustments with breath holds during exercise have been noted to reduce [??][O.sub.2] . The linear rise during the last 3 min with ischemia reflects the decreasing mechanical efficiency and the progressive partial restoration of leg circulation. The peak 45 s after cuff deflation signifies lung reoxygenation of venous blood returning from the legs, extracting more [O.sub.2] to repay the aerobic and anaerobic deficit incurred during the prior ischemia. Most of the anaerobic deficit was repaid over the last 3 min of uncuffed exercise as [??][O.sub.2] returned to near baseline exercise levels. However, some residual debt repayment probably occurred during the postexercise rest because the repayment exceeded the deficit at the start of exercise by 423 mL (Figure 1) and the half-time of the off response (37 s) was significantly (p = 0.001) slower than the on-response (27 s); the latter value agreed with previous reports [27-28].
The estimated [CO.sub.2] capacitance value of 1.2 Lxmm[Hg.sup.-1] X[kg.sup.-1] is lower than that (1.6) interpolated for the same exercise workload from a report  during 15 min of hyperventilation, although values twice as high have also been reported . Capacitance values are directly related to the length of experiments, because more [CO.sub.2] is then washed out of slower compartments . Because leg perfusion was impaired during our experiments, one would have expected a relatively low capacitance value because [CO.sub.2] in blood and tissue of the legs are then washed out at a slower rate, being somewhat isolated from the lung. Another consideration is that [CO.sub.2]s change significantly slower than [O.sub.2]s, having a half-time of 4.0 min versus 0.5 min for [O.sub.2]s, based on studies on dogs by Farhi and Rahn . This finding suggests that part of the loss in [CO.sub.2]s following cuff deflation may be attributed to the hyperventilation during the prior ischemia.
After cuff deflation, the larger [CO.sub.2]s loss relative to [O.sub.2]s gain resulted from the HC[O.sub.3.sub.-] buffering of lactate entering the circulation. Correlation of lactate levels with excess [??][CO.sub.2] in relation to [??][O.sub.2] during and after heavy exercise resulted in the "anaerobic threshold" concept [32-33]. Excess [??][CO.sub.2] during exercise has also been used to estimate lactate accumulation in physically fit subjects  and cardiac patients . The elevated [??][CO.sub.2] and [CO.sub.2]s depletion is caused by carbonic acid, arising from the combination of [H.sup.+] with HC[O.sub.3.sup.-] dissolved [CO.sub.2] from the muscle tissue being transported to the lungs once circulation is restored; and elevated [[??].sub.E] . As shown by [[??].sub.E]/[??][CO.sub.2] in Figure 3, the metaboreflex ventilatory drive was quickly diminished after cuff deflation, but the drive was then taken over by the chemoreflex stimulated by elevated [H.sup.+] and P[CO.sub.2] in blood arriving at central chemoreceptors and continuing during subsequent rest.
In studies somewhat similar to this one, a rise of arterial blood lactate of [approximately equal to]4 mmol/L was reported 4 to 5 min after cuff deflation  and also a 4 mmol/L loss of plasma HC[O.sub.3.sub.-] . This amount of lactate release was incorporated into the model shown in Table 1 and Figure 4. If 4 mmol/L of lactate release from the legs to central circulation was entirely buffered by HC[O.sub.3.sub.-] during the 5 min postinflation period, it would amount to a [CO.sub.2]s loss of 4 mmol/L X 5.9 L X 22.3 mL/mmo1= 526 mL . This amount accounts for 75 percent of the 697 mL estimate. However, the ratio of [CO.sub.2] loss to [O.sub.2] gain of 2.6 (697/273) suggests that a part of the lactate may have been converted by oxidation, in addition to being buffered . These and other biochemical processes must have continued beyond the postexercise resting measurement period to fully restore [O.sub.2]s and [CO.sub.2]s to baseline levels of 1,331 and 6,827 mL, respectively. However, most of the excess [CO.sub.2] was eliminated by the time exercise stopped because [??][CO.sub.2] had returned to baseline (Figure 1). Without prolonged lactate turnover measurements, we can only generalize that the majority of the lactate was buffered in preference to other chemical pathways to account for the [CO.sub.2]s loss exceeding the [O.sub.2]s gain. Qualitatively, [[??].sub.E] increases during exercise with cuffs inflated, depleting [CO.sub.2]s, while the partially anaerobic exercise continues. When cuffs are deflated and after exercise stops, metabolic by-products from the legs returning to the central circulation keep ventilation elevated to repay [O.sub.2]s, while [CO.sub.2]s remains below baseline for a longer time.
Clearly, the assumptions in the total gas stores model demonstrated in Table 1 and Figure 4 will affect the absolute values and changes in gas store values. Some quantities, such as tissue water and arterial and venous blood volumes, are not easily measured and were taken from estimates in the literature. To quantify the effect of variations in these assumed values, in Table 2, we show changes in total [O.sub.2]s and [CO.sub.2]s resulting from variations in values from those used in Table 1 during the three exercise conditions. We varied indicated values for relevant physiological components individually, assuming the other variables remained constant. Table 2 indicates that calculations of total [O.sub.2]s and [CO.sub.2]s and phase differences in stores are most sensitive to values for Hb and reductions in Q during the ischemic phase. Any alveolar-arterial differences in P[O.sub.2] and P[CO.sub.2] greatly influence total stores, especially [CO.sub.2]s, but the effect on store differences is smaller, somewhat similar to changing values for the other components. Therefore, performing invasive measurements, including arterial and mixed venous blood gases and lactate, in more definitive future studies is important.
Most studies using cuffs to induce acute exercise ischemia have focused on the [[??].sub.E] response following cuff deflation to study [CO.sub.2] chemoreceptor response mechanisms. Data from some of these reports [23,36-37,39-40] allowed a gas store pattern estimation to compare with this study and are shown in Table 3. Generalizations from these limited data include (1) an inverse relationship between cuff pressure and [O.sub.2]s reduction during inflation, (2) a direct relationship between workload and the increase in [O.sub.2]s and reduction in [CO.sub.2]s after cuff deflation, and (3) the [CO.sub.2]s loss after cuff deflation exceeds the change during inflation and also exceeds the [O.sub.2]s gain in recovery. From the time trends in the present study and those prior studies where time resolution was presented [37,39], apparently during inflation, the decrease in [O.sub.2]s is attenuated as exercise duration increases. This finding is probably associated with the increasing [??][O.sub.2] required by the elevated [[??].sub.E] and extra muscular effort and partial restoration of leg blood flow that diminish the [O.sub.2]s deficit and increase the [CO.sub.2]s deficit. Apparently, leg cuff pressures must be >90 mmHg during exercise to affect measured V [CO.sub.2] and [??][O.sub.2] during exercise [41-42].
SUMMARY AND CONCLUSIONS
The events in these experiments can be described as a respiratory alkalosis during ischemia, followed by a metabolic acidosis after cuff deflation when metabolites from the anaerobic portion of leg work return to the central circulation. Changes in [O.sub.2]s depend mainly on perfusion through lung and tissue, while [CO.sub.2]s changes are primarily determined by [[??].sub.E] , venous blood redistribution, and HC[O.sub.3.sub.-] buffering of lactate. This study estimated that the ischemia required a repayment of 273 mL of [O.sub.2] and produced 697 mL of [CO.sub.2]. These values depend on workload, work duration with ischemia, the cuff pressure determining the perfusion impairment, and the intensity of the metaboreflex. The amount of anaerobic debt incurred and tolerated and the recovery from a given ischemic exercise scenario will depend on the aerobic fitness of the subject and related blood pressure reflex response. These factors must be considered if this form of exercise is further evaluated and implemented for rehabilitation.
We thank the subjects for their cooperation in making this study possible.
Jack Loeppky is now retired in Cranbrook, British Columbia, Canada.
This material was based on work supported by the Department of Veterans Affairs, Veterans Health Administration, Rehabilitation Research and Development Service, grant F4096R to Milton V Icenogle, MD, Cardiology Section, Veterans Integrated Service Network 18, Albuquerque, New Mexico.
The authors have declared that no competing interests exist.
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Abbreviations: ADS = anatomical dead space (mL), BE = base excess (measure of whole blood buffer base [mmol/L]), [CO.sub.2] = carbon dioxide, [CO.sub.2]s = [CO.sub.2] stores (mL), f = breathing frequency (breaths/min), FI[O.sub.2] = fraction of inspired oxygen, [H.sup.+] = hydrogen ion concentration (mnol/L), Hb = hemoglobin concentration (g%), HC[O.sub.3.sub.-] = bicarbonate concentration (mmol/L), [O.sub.2] = oxygen, [O.sub.2]s = [O.sub.2] stores (mL), PA[CO.sub.2] = partial pressure of alveolar [CO.sub.2] (mmHg), PA[O.sub.2] = partial pressure of alveolar [O.sub.2] (mmHg), [P.sub.B] = barometric pressure, P[CO.sub.2] = partial pressure of [CO.sub.2] (mmHg), pHa = arterial pH, P[O.sub.2] = partial pressure of [O.sub.2] (mmHg), Q = cardiac output (L/min), RER = respiratory exchange ratio ([CO.sub.2] output/[O.sub.2] uptake), VA = alveolar ventilation ([L/min] body temperature, ambient pressure, saturated), [??][CO.sub.2] = [CO.sub.2] output ([mL/min] standard temperature and pressure, dry), [[??].sub.E] = pulmonary ventilation ([L/min] body temperature, ambient pressure, saturated), [??][O.sub.2] = [O.sub.2] uptake ([mL/min] standard temperature and pressure, dry), [??][O.sub.2.max] = maximal [??][O.sub.2].
Jack A. Loeppky, PhD; (1) * Burke Gurney, PhD; (2) Milton V Icenogle, MD (3)
(1) Research Service, Department of Veterans Affairs Medical Center (VAMC), Albuquerque, NM, (2) Dppartment of Orthopaedics and Division of Physical Therapy, University of New Mexico Health Sciences Center, Albuquerque, NM, (3) Cardiology Section, VAMC, Albuquerque, NM
* Address all correspondence to Jack A. Loeppky, PhD; Research Service, VAMC, 1501 San Pedro Dr SE, Albuquerque, NM 87108; 250-489-4597; fax: 250-489-8256. Email: Ioe11pkviatelus.net or IogRpkvna,unm.edu
Table 1. Estimated oxygen stores ([O.sub.2]s) and carbon dioxide stores ([CO.sub.2]s) (milliliters) during three conditions (A, B, and, C) of 15 min exercise: 5th min baseline, 5th min of inflation, and 5th min after cuff deflation, respectively. [O.sub.2] Condition Location P[O.sub.2] Stores A. 5th Min Baseline Arterial 84.1 232 (BE = -1.8 mmol/L, Venous 28.9 486 pHa = 7.420, Q x = 15 L/min) Tissue 28.9 37 Lung 84.1 576 Total -- -- 1,331 B. 5th Min Cuff Inflation Arterial 92.1 293 (BE = -1.8 mmol/L, Venous 25.3 375 pHa = 7.462, Q x = 14 L/min) Tissue 25.3 32 Lung 92.1 631 Total -- -- 1,331 B - A -- -- 0 C. 5th Min Cuff Deflation * Arterial 86.6 232 (BE = -5.8 mmol/L, Venous 30.3 486 pHa = 7.370, [??] = 15 L/min) Tissue 30.3 38 Lung 86.6 593 Total -- -- 1,349 C--B -- -- 18 C--A -- -- 18 [CO.sub.2] Condition P[CO.sub.2] Stores A. 5th Min Baseline 33.8 479 (BE = -1.8 mmol/L, 45.4 2,293 pHa = 7.420, [??] = 15 L/min) 45.4 3,824 33.8 231 Total -- 6,827 B. 5th Min Cuff Inflation 28.9 559 (BE = -1.8 mmol/L, 44.7 2,146 pHa = 7.462, Q x = 14 L/min) 44.7 3,760 28.9 198 Total -- 6,663 B--A -- -164 C. 5th Min Cuff Deflation * 31.4 402 (BE = -5.8 mmol/L, 42.7 1,986 pHa = 7.370, Q x = 15 L/min) 42.7 3,595 31.4 215 Total -- 6,198 C--B -- -465 C--A -- -629 Note: [O.sub.2]s and [CO.sub.2]s are based on model given in "Methods" of main text with assumptions: * Total blood volume = 5,900 mL. * Venous volume = total x 0.8 = 4,720 mL; arterial = total x 0.2 = 1,180 mL in conditions A and C. * 300 mL was shifted from venous to arterial compartment in condition B; i.e., venous = 4,420 mL and arterial = 1,480 mL. * Adjusted for BE = -4.0 mmol/L. BE = base excess (measure of whole blood buffer base), pHa = arterial pH (negative log of [H.sup.+] in arterial blood), P[CO.sub.2] = partial pressure of carbon dioxide, P[O.sub.2] = partial pressure of oxygen, [??] = cardiac output. Table 2. Effect on total gas stores of variations in assumed values for gas stores model during three conditions A, B, and C of 15 min exercise: 5th min baseline, 5th min of cuff inflation, and 5th min after cuff deflation, respectively. Value Variable Value Condition Change Functional Residual 4.0 A, B, C [+ or -]10% Capacity (L) Blood Volume (L) 5.9 A, B, C [+ or -]10% Hb (g%) 15.0 A, B, C [+ or -]10% Alv-Art Diff (mmHg) P[CO.sub.2] & A, B, C 3, 13 P[O.sub.2] = 0 [H.sup.+] (nmol/L) pHa = 7.42 A, B, C [+ or -]10% at base [??] Decrease (L/min) 1.0 B 0 & 2 BE Decrease (mmol/L) 4.0 C -3 & -5 [O.sub.2]s Difference Total* (%) Diff ([double Variable ([ddager]) dagger]) Functional Residual 60 (4.5) 5 Capacity (L) Blood Volume (L) 69 (5.2) 8 Hb (g%) 118 (8.8) 2 Alv-Art Diff (mmHg) 112 (8.4) 10 [H.sup.+] (nmol/L) 4 (0.3) 2 [??] Decrease (L/min) 37 (2.8) 37 BE Decrease (mmol/L) 2 (0.1) 2 [CO.sub.2]s Difference Total* (%) Diff (double Variable (dagger) dagger) Functional Residual 21 (0.3) 3 Capacity (L) Blood Volume (L) 263 (4.0) 19 Hb (g%) 110 (1.7) 34 Alv-Art Diff (mmHg) 536 (8.2) 32 [H.sup.+] (nmol/L) 193 (2.9) 18 [??] Decrease (L/min) 141 (2.1) 141 BE Decrease (mmol/L) 73 (1.2) 73 Note: [CO.sub.2]s decreases 73 mL per 1.0 mmol/L decrease in BE. * Mean absolute differences in total gas stores (milliliters) from values in Table 1 (see main text). ([dagger]) These mean differences as % of values in Table 1. ([double dagger]) Mean of differences in gas store changes between conditions from those in Table 1. Alv-Art = alveolar-arterial, BE = base excess (measure of whole blood buffer base), [CO.sub.2]s = carbon dioxide stores, diff = differences, [H.sup.+] = hydrogen ion concentration, Hb = hemoglobin concentration, [O.sub.2]s = oxygen stores, P[CO.sub.2] = partial pressure of carbon dioxide, pHa = arterial pH, P[O.sub.2] = partial pressure of oxygen, [??] = cardiac output. Table 3. Cumulative time-integrated oxygen uptake ([??][O.sub.2]), carbon dioxide output ([??][CO.sub.2]), and pulmonary ventilation ([[??].sub.E]) differences from baseline during leg cuff inflation and after cuff deflation. Work Time Cuff Before Pressure Inflation Studies n Work (W) (mmHg) (min) Stegemann, 1963  None 0 250-300 0 given None "mild" 250-300 20 given Asmussen & Nielsen, 1 31 300-350 10-15 1964 ([CO.sub.2] 4 62 300-350 10-15 added to maintain 2 124 300-350 10-15 PA[CO.sub.2])  Sargeant et al., 5 100 250 0 1981  Stanley et al., 8 49 200 6 1985  8 98 200 6 Roth et al., 9 [approximately 200 6 1988  equal to]17 This Study 6 75 140 5 Inflation Inflation [??][O.sub.2] Studies n Time (min) (mL) Stegemann, 1963  None 10 -495 given None 6 -233 given Asmussen & Nielsen, 1 3 -358 1964 ([CO.sub.2] 4 3 -984 added to maintain 2 2 -1,183 PA[CO.sub.2])  Sargeant et al., 5 2 -130 1981  Stanley et al., 8 2 -274 1985  8 2 -428 Roth et al., 9 2 -740 1988  This Study 6 5 282 (2) -42 Inflation [??] [CO.sub.2] [[??].sub.E] Studies n (mL) (L) Stegemann, 1963  None -387 -13 given None -121 0 given Asmussen & Nielsen, 1 -- -- 1964 ([CO.sub.2] 4 -- -- added to maintain 2 -- -- PA[CO.sub.2])  Sargeant et al., 5 350 32 1981  Stanley et al., 8 129 8 1985  8 106 10 Roth et al., 9 -- -- 1988  This Study 6 932 55 167 10 Deflation Recovery [??][O.sub.2] Studies n Time (min) (mL) Stegemann, 1963  None 20 448 given None 10 228 given Asmussen & Nielsen, 1 -- -- 1964 ([CO.sub.2] 4 -- -- added to maintain 2 -- -- PA[CO.sub.2])  Sargeant et al., 5 4 770 1981  Stanley et al., 8 4 547 1985  8 4 643 Roth et al., 9 4 1,440 1988  This Study 6 5 654 Deflation Recovery [??][O.sub.2] Studies n Time (min) (mL) Stegemann, 1963  None 20 448 given None 10 228 given Asmussen & Nielsen, 1 -- -- 1964 ([CO.sub.2] 4 -- -- added to maintain 2 -- -- PA[CO.sub.2])  Sargeant et al., 5 4 770 1981  Stanley et al., 8 4 547 1985  8 4 643 Roth et al., 9 4 1,440 1988  This Study 6 5 654 Note: See legend to Figure 2 for "predicted" values for [??][O.sub.2] and [??][CO.sub.2] for this study; here all "predicted" values were assumed equal to baseline. 1. Stegemann J. [On the mechanism of pulse frequency regulation by metabolism. I. The influence of metabolism in a muscle group isolated from the circulation on the behavior of the pulse frequency]. Pflugers Arch Gesamte Physiol Menschen Tiere. 1963;276:481-92. German. [PMID: 13983630] 2. Asmussen E, Nielsen M. Experiments on nervous factors controlling respiration and circulation during exercise employing blocking of the blood flow. Acta Physiol Scand. 1964;60:103-11. [PMID: 14131818] 3. Sargeant AJ, Rouleau MY, Sutton JR, Jones NL. Ventilation in exercise studied with circulatory occlusion. J Appl Physiol. 1981;50(4):718-23. [PMID: 6790486] 4. Stanley WC, Lee WR, Brooks GA. Ventilation studied with circulatory occlusion during two intensities of exercise. Eur J Appl Physiol Occup Physiol. 1985;54(3): 269-77. [PMID: 3933976] 5. Roth DA, Stanley WC, Brooks GA. Induced lactacidemia does not affect postexercise [O.sub.2] consumption. J Appl Physiol. 1988;65(3):1045-49. [PMID: 3182473] [CO.sub.2] = carbon dioxide, PA[CO.sub.2] = partial pressure of alveolar [CO.sub.2] (mmHg).
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|Author:||Loeppky, Jack A.; Gurney, Burke; Icenogle, Milton V.|
|Publication:||Journal of Rehabilitation Research & Development|
|Date:||Sep 1, 2008|
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