Printer Friendly

Effects of Achyranthes bidentata polysaccharide on growth performance, immunological, adrenal, and somatotropic responses of weaned pigs challenged with Escherichia coli lipopolysaccharide.

ABSTRACT : A study was conducted with 48 weaned barrows (28 [+ or -] 3 d, 8.45 [+ or -] 0.14 kg) to determine the effect of Achyranthes bidentata polysaccharide (ABPS) supplementation on pig performance, immunological, adrenal and somatotropic responses following Escherichia coli lipopolysaccharide (LPS) challenge. The experiment was a 2x2 factorial design; the main factors included diet (supplementation with 0 or 500 mg/kg ABPS) and immunological challenge (LPS or saline). On d 14 and 21 of the trial, pigs were given an intraperitoneal injection with either 100 [micro]g/kg BW of LPS or an equivalent amount of sterile saline. Blood samples were obtained 3 h after injection for analysis of tumor necrosis factor-[alpha] (TNF-[alpha]), prostaglandin [E.sub.2] (PG[E.sub.2]), cortisol, growth hormone (GH), insulin-like growth factor (IGF)-I and immunoglobulin G (IgG). On d 2 after LPS challenge, peripheral blood lymphocyte proliferation (PBLP) was measured. LPS administration decreased average daily feed intake (ADFI) (p<0.05), had a tendency to decrease average daily gain (ADG) (p<0.10) during both the first and second challenge periods and increased (p<0.05) feed:gain ratio only during the first challenge period. ABPS tended to improve ADG (p<0.10) during the first challenge period, and improved ADG (p<0.05) and tended to improve ADFI (p<0.10) during the second challenge period. ABPS did not affect feed:gain ratio. An interaction (p<0.05) between LPS challenge and diet was observed for the plasma concentrations of TNF-[alpha], PG[E.sub.2] and cortisol after both LPS challenges such that, among LPS-treated pigs, pigs fed the ABPS diet were lower for these indices than those receiving the control diet. In contrast, pigs fed the ABPS diet had higher IGF-I (p<0.05) compared with those fed the control diet. No effect of diet, LPS challenge or both on GH and IgG was observed after both LPS administrations. LPS challenge increased PBLP when these cells were incubated with 8 [micro]g/ml of LPS during both the challenge periods, and did likewise when incubated with 8 [micro]g/ml of concanavalin A only after the first challenge. ABPS had no effect on PBLP. These data demonstrate that ABPS alters the release of pro-inflammatory cytokines following an immunological challenge, which might enable pigs to achieve better performance. (Key Words : Achyranthes Bidentata Polysaccharide, Lipopolysaccharide, Inflammatory Response, Weaned Pigs)

INTRODUCTION

In swine production, the continual exposure of weanling piglets to a wide variety of microorganisms will lead to an immunological challenge, which can result in a series of physiological changes including temporary fever, depressed feed intake and activation of the immune system (Kelley et al., 1994). Consequently, the immunological challenge results in poor growth on animals and increases economic loss for pig producers. Johnson (1997) and Webel et al. (1997) propose that these changes are mostly ascribed to the release of pro-inflammatory cytokines, including tumor necrosis factor (TNF), interleukin (IL)-1 and IL-6. Overproduction of these pro-inflammatory cytokines has negative effects on growth and feed efficiency. Therefore, using immunomodulators to modulate the secretion of these cytokines is considered as a potential means to mitigate the negative effects induced by an immunological challenge (Lang et al., 1996).

Achyranthes bidentata polysaccharide (ABPS), a gray-white powder, which is isolated from the root of Chinese medicinal herb Achyranthes bidentata Blume, is composed of fructose and glucose residues and the molar ratio is 8:1 (Chen et al., 2005). ABPS is a graminans-type fructan that contains a [beta]-D fructofuranosyl backbone having residues linked (2 [right arrow] 1)- and (2 [right arrow] 6)- with branches and an [alpha]-D-glucopyranose residue on the nonreducing end of the fructan chain (Jin et al., 2007). Compared with other polysaccharides, ABPS has a smaller molecular mass. In humans and rodents, it has been used as an immune modulator (Li and Li, 1997; Shao et al., 2002; Jin et al., 2007). Because of its traits of natural origin, as well as diverse pharmacological effects, producing no drug residues and low side effects, ABPS is an attractive alternative to antibiotics. Within our knowledge, no study has been conducted to observe the effect which ABPS exerts its activities in pigs. Therefore, this study was conducted to evaluate the effects of dietary ABPS supplementation on performance, as well as the immunological, adrenal, and somatotropic response in weaned pigs challenged with Escherichia coli lipopolysaccharide (LPS).

MATERIALS AND METHODS

Experimental design

Our experimental protocol was approved by the Animal Care and Use Committee of Hubei Province. A total of 48 crossbred (Duroc x Large White x Landrace) male pigs weaned at 28 [+ or -] 3 d of age (8.45 [+ or -] 0.14 kg) were randomly allotted to one of four treatments by initial BW in a 2 x 2 factorial arrangement that included a dietary addition of ABPS (0 or 500 mg/kg) and LPS challenge (with or without).

Pigs were housed in 1.20 x 1.10 m pens with six replicate pens per treatment with two pigs per pen. Each pen was equipped with plastic slotted floor, and a feeder and a nipple waterer to allow pigs ad libitum access to feed and water. The temperature in the room was controlled at 25-27[degrees]C by air condition. The basal diet (Table 1) was formulated to meet or exceed NRC (1998) requirements for all nutrients. ABPS, which contained polysaccharide no less than 90% and had a molecular weight of 2,680, was purchased from Zhejiang Jingxin Pharmaceutical Company (Zhejiang, China). Pigs were weighed and feed disappearance was measured on days 0, 14, 21 and 28 throughout the 28-day trial.

Immunological challenge model

On d 14 and 21 of the trial, the challenged group was injected intraperitoneally with LPS (Escherichia coli Serotype O55:B5; Sigma Chemical Co., St. Louis, MO, USA) at 100 [micro]g/kg BW and the unchallenged group was administrated an equivalent amount of 0.9% (wt/vol) NaCl solution. The LPS was dissolved in sterile 0.9% NaCl solution such that 0.2 ml of solution/kg of BW would achieve the desired dosage.

Blood sample collections and assays

On d 14 and 21, 3 h after the LPS or saline administration, blood samples (all pigs) were respectively collected into heparinized vacuum tubes (Becton Dickinson Vacutainer System; Franklin Lake, NJ) and centrifuged (3,500xg for 10 min) to separate plasma, and then stored at -80[degrees]C until analysis.

Plasma tumor necrosis factor-[alpha] (TNF-[alpha]) was analyzed using a commercially available ELISA kits (R&D Systems Inc., Minneapolis, MN). Minimum detectable concentration was 3.7 pg/ml with an intra- and inter-assay CV were 4.9% and 8.9%. The average recovery of porcine TNF-[alpha] in porcine serum is 95%.

Plasma prostaglandin [E.sub.2] (PG[E.sub.2]) was analyzed using a commercially available [sup.125]I RIA kit (College of Medical Science of Suzhou University, Jiansu, China), which we have also previously validated in pigs (Liu et al., 2003). Minimum detectability of PG[E.sub.2] was 6.25 pg/ml with an intra-assay CV less than 10%.

Plasma cortisol was analyzed using a Coat-a-CountTM assay kit (Diagnostic Products, Los Angeles, CA). Minimum detectable dose of cortisol was 2 ng/ml. Intra-and inter-assay CV were 4.6% and 9.0%, respectively.

Plasma growth hormone (GH) was measured using a commercially available porcine GH [sup.125]I RIA kit (Linco Research, Inc., St. Charles, MO). The minimum detection limit was 1 ng/ml, with an intra-assay CV of 4.0%.

Plasma insulin-like growth factor-I (IGF-I) was determined by using a commercially available porcine IGF-1 IRMA kit (Diagnostic Systems Laboratories, Inc.). The minimum detection limit was 2 ng/ml, with an intra-assay CV of 3.9%.

IgG concentration was determined using a radial immunodiffusion test kit according to the methods of Hicks et al. (1998) as a representative humoral immune response. Five microliters of standard solutions and diluted plasma samples were pipetted to a separately identified well of the test plates. The plate was securely covered and placed in a 37[degrees]C, humidified incubator for 48 to 72 h. After incubation, plates were removed and placed over a source of illumination to clearly see precipitin rings. The external diameters of the rings were measured to the nearest 0.1 mm using the scale provided. A reference curve was plotted using the diameters measured from standard solutions. From the reference curve, the IgG concentration of each diluted test sample was calculated by multiplying the concentration read from the curve by the dilution factor to obtain the actual concentration.

All blood related measurements were analyzed in duplicate.

Lymphocyte proliferation

Lymphocyte proliferation was measured using a colorimetric assay, with 3-(4,5-dimethlthiazol-2-yl)-2,5-diphenyltetrazolium bromide (M-2128, Sigma Chemical Inc., St. Louis, USA) in cultures of purified peripheral blood mononuclear cells according to the method of Liu et al. (2003) and Kong et al. (2007). Briefly, mononuclear cells were collected by gradient centrifugation from the peripheral blood obtained d 2 after the first and the second LPS injection, at 3,000xg for 30 min. The cells were washed three times, and then suspended in RPMI-1640 complete culture medium supplemented with 10% (vol/vol) heat-inactivated fetal calf serum, 100 U/ml of penicillin, 100 [micro]g/ml of streptomycin and 25 mM N-(2-hydroxyethyl)-piperazine-N'-2-ethane-sulfonic acid. Cell activity was detected by trypan blue dye exclusion, and the cell density was counted to adjust to 2x [10.sup.6] cells/ml culture medium. Then the cellular suspension was added to 96-well microtiter plates with a total culture volume of 200 [micro]l.

Lymphocyte mitogen concanavalin A (ConA; Type IV, C-2010, Sigma Chemical Inc., St. Louis, USA) or LPS was added at a final concentration of 8 [micro]g/ml culture medium, and then the plates were incubated at 37[degrees]C in a 5% C[O.sub.2] incubator. After 66 h of incubation, 10 [micro]l MTT solution (5 mg MTT/ml in 1/15 M phosphate-buffered saline, pH 7.6) was added to each well and the plates were incubated at 37[degrees]C for another 6 h. At the end of incubation, 100 [micro]l of a 10% sodium dodecyl sulfate in 0.04 M HCl solution was added to lyse the cells and solubilize the MTT crystals. Plates were read at 570 nm using an automated microplate reader (Bio-Rad, Model 550, Hercules, CA). Lymphocyte proliferation was expressed as a stimulation index, which was calculated as the absorbance of wells incubated with ConA or LPS divided by the absorbance of wells incubated without ConA or LPS.

Statistical analysis

All data were analyzed by ANOVA using the GLM procedures of SAS (SAS Inst. Inc., Cary, NC) appropriate for a factorial arrangement of treatments in a randomized complete block design. The statistical model consisted of the effects of challenge (saline or LPS), diet (ABPS or no ABPS) and their interactions. Pen was used as experimental unit for the performance data, whereas individual pig data were used as the experimental unit for blood analysis and measurement of lymphocyte proliferation. A level of p<0.05 was used as the criterion for statistical significance, whereas p<0.10 was taken to indicate a statistical trend.

RESULTS

Growth performance

There was no LPS challenge x diet interaction for average daily gain (ADG), average daily feed intake (ADFI) and feed/gain observed throughout the 28-d trial (Table 2). Prior to LPS challenge (from d 0 to 14), dietary treatment did not affect growth performance. During the first challenge period (from d 14 to 21), pigs fed ABPS tended to have a 10.0% higher ADG (p<0.10) than the control pigs, but ADFI and feed:gain were not affected. Correspondingly, LPS challenge reduced ADG (p<0.05) by 21.7% and ADFI (p<0.05) by 11.1%, and increased feed:gain (p<0.05) by 13.6% compared to the saline-injected pigs regardless of dietary treatment. During the second challenge period (from d 21 to 28), supplementation of ABPS increased ADG (p<0.05) by 8.9% and tended to increase ADFI (p<0.10) by 5.6%; whereas LPS challenge had a tendency to decrease ADG (p<0.10) by 7.7% and decreased ADFI (p<0.05) by 7.6%. In the whole performance trial (from d 0 to 28), there was no significant affect on ADG, ADFI and feed:gain observed by addition of ABPS, but LPS challenge reduced ADG (p<0.05) by 9.5% and ADFI (p = 0.05) by 6.2%, and had a tendency to increase feed:gain (p<0.10) by 3.7% compared with the saline-treated pigs.

Lymphocyte proliferative and humoral responses

The results of lymphocyte proliferation are shown in Table 3. There was no LPS challenge x diet interaction observed for lymphocyte proliferation when incubated with either LPS or ConA. Dietary treatment had no effect on lymphocyte proliferation. LPS challenge increased (p<0.05) lymphocyte proliferation when incubated with 8 [micro]g/ml LPS after the first and second LPS challenges, and did likewise with 8 [micro]g/ml ConA only after the first LPS challenge.

The concentration of serum IgG was measured to represent the humoral immune response, and the results are presented in Table 4. There was neither LPS challenge nor diet effect on the concentration of serum IgG after the first and the second LPS challenge.

Plasma TNF-[alpha], PG[E.sub.2], cortisol, GH, and IGF-I

As indicated by Table 5, after the first and second LPS challenges, an interaction between ABPS and LPS was observed for all plasma indices with the exception of GH. Plasma TNF-[alpha] (p<0.05), PG[E.sub.2] (p<0.10) and cortisol (p<0.10) responses to the LPS challenge were lower in pigs receiving the ABPS diet than the LPS-treated pigs fed the control diet, whereas there was no difference for these indices response in the saline-injected pigs. In contrast, plasma IGF-I in pigs fed the ABPS diet was higher (p<0.05) than in those fed the control diet among LPS-injected pigs, whereas there was no difference among saline-injected pigs. No effects of diet, LPS challenge or both on plasma GH concentration were observed following both the first and the second LPS challenge.

DISCUSSION

In the present study, we used the well-recognized model for inducing sickness in pigs by injecting LPS (Johnson and von Borell, 1994; Lien et al., 2005) which is a component of the outer membrane of gram-negative bacteria, to determine the effect of ABPS supplementation on immunological challenge. Until now, few researches were conducted to evaluate the effect of ABPS on growth performance of livestock. In our study, before LPS challenge, dietary treatment had no effect on pig performance. This finding is similar to Chen (2002) who reported that there was no effect on broiler performance when ABPS added to 200 mg/kg. However, pigs fed 500 mg/kg ABPS had higher weight gain during both LPS challenge periods and higher feed intake during the first LPS challenge period compared with those fed the control diet, which indicates the importance of ABPS supplementation under stress, infection and diseases. The LPS challenge significantly decreased pig weight gain and feed intake, and this finding is consistent with some previous studies in pigs (Lee et al., 2000b; Liu et al., 2003; Mao et al., 2005).

In the current study, the circulating concentrations of the inflammatory indices TNF-[alpha] and PG[E.sub.2] were increased after LPS injection, which is consistent with Lee et al. (2000a) and Liu et al. (2008a, b). It is well known that, during an immunological challenge, one of the first responses of an animal is to release pro-inflammatory cytokines such as IL-1[beta] and TNF-[alpha] from macrophages (Spurlock, 1997), which will induce an increase in the secretion of PG[E.sub.2] in muscle (Hellerstein et al., 1989) and an activation of the immune system. This response directs nutrients away from tissue growth to support immune function (Spurlock, 1997), and finally decreases the efficiency of nutrient utilization for growth. The pigs fed ABPS had lower concentrations of TNF-[alpha] and PG[E.sub.2] than did pigs fed the control diet among the LPS-challenged pigs, which indicates an anti-inflammatory role of ABPS. Until now, no other research was conducted to evaluate the anti-inflammatory role of ABPS. The exact mechanism(s) by which ABPS exerts its anti-inflammatory role on the animals is unclear. However, there are three possible pathways for anti-inflammatory effects of ABPS.

First of all, ABPS may inhibit pro-inflammatory cytokine synthesis through the synthesis of anti-inflammatory cytokines. Anti-inflammatory cytokines such as IL-10, by suppressing the activity of the signal transduction of nuclear transcription factor [kappa]B which is a major transcription factor of pro-inflammatory cytokines (Schottelius et al., 1999) inhibit the synthesis of proinflammatory cytokine to maintain the balance between the pro- and anti-inflammatory mediators (Hoqaboam et al., 1998). Secondly, ABPS may increase the synthesis of IL-1 receptor antagonist. IL-1 receptor antagonist is a competitive inhibitor of interleukin-1 that binds to type I interleukin-1 receptors (Poutsiaka et al., 1993). Regretfully, we did not measure the anti-inflammatory cytokines and interleukin receptor antagonists in the current study. Finally, ABPS may inhibit pro-inflammatory cytokines synthesis by decreasing the production of arachidonic acid metabolites such as prostaglandins (PG[E.sub.2] in the current study). Prostaglandins play a major role in the inflammatory and immune responses and are capable to decrease cytokines production (Calder, 1997).

In our study, that ABPS supplementation significantly suppressed the increase of pro-inflammatory cytokine TNF-[alpha] and PG[E.sub.2] release, which indicates less activation of the immune system, and consequently more nutrients to support tissue growth. This may partially explain why pigs fed 500 mg/kg ABPS had higher gain and feed intake compared with the control.

Besides depressed gain and feed intake, a reduction in plasma IGF-I was observed in pigs administrated with LPS. The study of Hasselgren (1993) suggested that the decrease in IGF-I was indicative of the repartitioning of nutrients away from normal growth to the immune response after LPS challenge. Soto et al. (1998) also proposed that the decrease in release of IGF-I was an important causative factor of decreased growth during an immunological challenge. Therefore, that the significantly mitigated reduction of plasma IGF-I due to addition of ABPS observed in our study may be an indication that ABPS supplementation alleviates the alterations in somatotropic axis after an LPS challenge, which might have been associated with the improved performance in pigs supplemented with ABPS compared with control pigs.

In the present study, in those LPS-challenged pigs, feeding ABPS partially attenuated the reduction of plasma IGF-I, concurrent with lower plasma TNF-[alpha] levels compared with the control. Therefore, the effect of ABPS on alleviating plasma IGF-I reduction seems to be closely associated with the decrease of pro-inflammatory cytokine release, such as TNF-[alpha]. McCarthy et al. (1995) proposed that anorexia induced by pro-inflammatory cytokines after LPS challenge was associated with the suppressed release of IGF-I. Accordingly, in our study, those LPS-challenged pigs fed with ABPS mitigated the decrease in feed intake, correspondingly attenuated the reduction of IGF-I. This may also explain why pigs fed ABPS had a higher feed intake compared with control pigs.

Pro-inflammatory cytokines are not only primarily associated with immune function, but also have the potential to change many aspects of neuroendocrine function, including the hypothalamic-pituitary-adrenal axis (HPAA) (Mandrup-Poulsen et al., 1995). Pro-inflammatory cytokines have been shown to stimulate neurons in the hypothalamus to release corticotropin-releasing hormone (Perlstein et al., 1993), which stimulated the adrenal cortex to produce cortisol (George and Chrousos, 1995). Concurred with aforesaid viewpoint, in the current study, we observed the increase in TNF-[alpha] induced by LPS challenge was concomitant with a rapid elevation of plasma cortisol, which is in agreement with previous study of Liu et al. (2003). However, feeding ABPS significantly decreased the level of plasma pro-inflammatory cytokine TNF-[alpha] and cortisol induced by LPS challenge. Therefore, ABPS may decrease the production of cortisol by suppressing pro-inflammatory cytokine TNF-[alpha] release.

In our study, ABPS supplementation decreased the release of pro-inflammatory cytokine TNF-[alpha] and PG[E.sub.2] in LPS-treated pigs, but did not reduce the production of TNF-[alpha] and PG[E.sub.2] in saline-injected pigs, indicating that ABPS mitigated the excessive activation of immune system in animals subjected to immunological challenge, but did not affect the immune function of normal animals. Furthermore, we did not observe an effect of ABPS on the concentration of IgG and lymphocyte proliferation in the current study. So ABPS did not decrease the immune function of normal animals. Therefore, it is rational to say that it is not bad at least to animals to fed ABPS when pigs are challenged by infectious diseases. Further evaluation of effects of ABPS on growth and inflammatory response in pigs challenged by infectious diseases is warranted before it is used in commercial practice.

IMPLICATIONS

The results of current study indicate that supplementation with achyranthes bidentata polysaccharide to pigs diet is able to relax the immunological and adrenal response to an Escherichaia coli lipopolysaccharide challenge by mediating the release of pro-inflammatory cytokines, and make pigs achieve better growth performance finally. However, source of achyranthes bidentata polysaccharide obtained from different methods or producers may vary in their structure, chemical composition, or both, and further evoke different effects on pigs. Accordingly, further investigation is warranted to focus on not only the performance and immune effect of achyranthes bidentata polysaccharide, but also the method producing the polysaccharide when it is added to animal diets.

ACKNOWLEDGMENT

The authors express their gratitude to the National Natural Science Foundation of China (30500362), the Youth Scholars Foundation of Wuhan, China (20055003059-46) and the Natural Science Foundation of Hubei Province (2005ABA091) for the financial supports.

Received December 20, 2007; Accepted March 17, 2008

REFERENCES

Calder, P. C. 1997. n-3 Polyunsaturated fatty acids and immune cell function. Adv. Enzyme Reg. 37:197-237.

Chen, H. L. 2002. Studies on the extraction, immunomodulating activities of Chinese Herbal polysaccharides and approach to the mechanism. Ph.D. Dissertation, Chinese Academy of Agriculture Science, Beijing, China.

Chen, X, M., Y. J. Xu and G.. Y. Tian. 2005. Physical-chemical properties and structure elucidation of ABPS isolated from the root of achyranthes bidentata. Acta Pharm Sinica 40:32-35 (Chinese).

George, P. and M. D. Chrousos. 1995. The hypothalamic-pituitary-adrenal axis and immune-mediated inflammation. N. Engl. J. Med. 332:1351-1363.

Hasselgren, P. O. 1993. Protein metabolism in sepsis. R. G. Landes Co., Austin, TX.

Hellerstein, M. K., S. N. Meydani, M. Meydani, K. Wu and C. A. Dinarello. 1989. Interleukin-1 induced anorexia in the rat. Influence of prostaglandins. J. Clin. Invest. 84:228-235.

Hicks, T. A., J. J. McGlone, C. S. Whisnant, H. G. Kattesh and R. L. Norman. 1998. Behavioral, endocrine, immune, and performance measures for pigs exposed to acute stress. J. Anim. Sci. 76:474-483.

Hoqaboam, C. M., M. L. Steinhauser, H. Schock, N. Lukacs, H. Schock, N. Lukacs, R. M. Strieter, T. Standiford and S. L. Kunkel. 1998. Therapeutic effects of nitric oxide inhibition during experimental fecal peritonitis: Role of interleukin-10 and monocyte chemoattractant protein 1. Infect. Immun. 66: 650-655.

Jin, L. Q., Z. J. Zheng, Y. Peng, W. X. Li, X. M. Chen and J. X. Lu. 2007. Opposite effects on tumor growth depending on dose of achyranthes bidentata polysaccharides in C57BL/6 mice. Int. Immunopharmacol. 7:568-577.

Johnson, R. W. 1997. Inhibition of growth by pro-inflammatory cytokines: An integrated view. J. Anim. Sci. 75:1244-1255.

Johnson, R. W. and E. von Borell. 1994. Lipopolysaccharide-induced sickness behavior in pigs is inhibited by pretreatment with indomethacin. J. Anim. Sci. 72:309-314.

Kelley, K. W., R. W. Johnson and R. Dantzer. 1994. Immunology discovers physiology. Vet. Immunol. Immunopathol. 43:157-165.

Kong, X. F., Y. L. Yin, G. Y. Wu, H. J. Liu, F. G. Yin, T. J. Li, R. L. Huang, Z. Ruan, H. Xiong, Z. Y. Deng, M. Y. Xie, Y. P. Liao and S. W. Kim. 2007. Dietary supplementation with acanthopanax senticosus extract modulates cellular and humoral immunity in weaned piglets. Asian-Aust. J. Anim. Sci. 20:1453-1461.

Lang, C. H., J. Fan, R. Cooney and T. C. Vary. 1996. Interleukin-1 receptor antagonist attenuates sepsis-induced alterations in the IGF system and protein synthesis. Am. J. Physiol. 270:E430-E437.

Lee, D. N., T. F. Shen, H. T. Yen, C. F. Weng and B. J. Chen. 2000a. Effects of chromium supplementation and lipopolysaccharide injection on the immune responses of weanling pigs. Asian-Aust. J. Anim. Sci. 13:1414-1421.

Lee, D. N., C. F. Weng, H. T. Yen, T. F. Shen and B. J. Chen. 2000b. Effects of chromium supplementation and lipopolysaccharide injection on physiological responses of weanling pigs. Asian-Aust. J. Anim. Sci. 13:528-534.

Li, Z. K. and D. D. Li. 1997. The immunomodulatory effect of achyranthes bidentata polysaccharides. Acta Pharm Sinica 32: 881-887 (Chinese).

Lien, T. F., K. H. Yang and K. J. Lin. 2005. Effects of chromium propionate supplementation on growth performance, serum traits and immune response in weaned pigs. Asian-Aust. J. Anim. Sci. 18:403-408.

Liu, Y. L., J. J. Huang, Y. Q. Hou, H. L. Zhu, S. J. Zhao, B. Y. Ding, Y. L. Yin, G. F. Yi, J. X. Shi and W. Fan. 2008a. Dietary arginine supplementation alleviates intestinal mucosal disruption induced by Escherichia coli lipopolysaccharide in weaned pigs. Br. J. Nutr. doi:10.1017/S0007114508911612.

Liu, Y. L., D. F. Li, L. M. Gong, Z. Y. Feng, G. F. Yi, A. M. Gaines and J. A. Carroll. 2003. Effects of fish oil supplementation on performance as well as immunological, adrenal and somatotropic responses of weaned pigs after Escherichia coli lipopolysaccharide challenge. J. Anim. Sci. 81:2758-2765.

Liu, Y. L., J. Lu, J. X. Shi, Y. Q. Hou, H. L. Zhu, S. J. Zhao, H. M. Liu, B. Y. Ding, Y. L. Yin and G. F. Yi. 2008b. Increased expression of the peroxisome proliferator-activated receptor? in the immune system of weaned pigs after Escherichia coli lipopolysaccharide challenge. Vet. Immunol. Immunopathol. 10.1016/j.vetimm.2008.02.014.

Mandrup-Poulsen, T., J. Nerup, J. I. Reimers, F. Pociot, H. U. Anderson, A. Karlsen, U. Bjerre and R. Bergholdt. 1995. Cytokines and the endocrine system. I. The immunoendocrine network. Eur. J. Endocrinol. 133:660-671.

Mao, X. F., X. S. Piao, C. H. Lai, D. F. Li, J. J. Xing and B. L. Shi. 2005. Effects of [beta]-glucan obtained from the Chinese herb Astragalus membranaceus and lipopolysaccharide challenge on performance, immunological, adrenal, and somatotropic responses of weanling pigs. J. Anim. Sci. 83:2775-2782.

McCarthy, H. D., S. Dryden and G. Williams. 1995. Interleukin-1[beta]-induced anorexia and pyrexia in rat: Relationship to hypothalamic neuropeptide Y. Am. J. Physiol. 269:E852-E857.

Perlstein, R. S., M. H. Whitnall, J. S. Abrams, E. H. Mougey and R. Neta. 1993. Synergistic roles of interleukin-6, interleukin-1, and tumor necrosis factor in the adrenocorticotropin response to bacterial lipopolysaccharide in vivo. Endocrinol. 132:946-952.

Poutsiaka, D. D., M. Mengozzi, B. Sinha and C. A. Dinarello. 1993. Cross-linking of the beta-glucan receptor on human monocytes results in interleukin-1 receptor antagonist but not interleukin-1 production. Blood 82:3695-3700.

Schottelius, A. J. G., M. W. Mayo, R. B. Sartor and A. S. Baldwin, Jr. 1999. Interleukin-10 signaling blocks inhibitor of kappa B kinase activity and nuclear factor kappa B DNA binding. J. Biol. Chem. 274:31868-31874.

Shao, S. J., L. Mai and Y. Chen. 2002. The effect of achyranthes bidentata polysaccharide on mice red blood cell immunological function. Chinese Remedies and Clinics, 5: 281-282 (Chinese).

Soto, L., A. I. Martin, S. Millan, E. Vara and A. Lopez-Calderon. 1998. Effects of endotoxin lipopolysaccharide administration on the somatotropic axis. J. Endocrinol. 159:239-246.

Spurlock, M. E. 1997. Regulation of metabolism and growth during immune challenge: An overview of cytokine function. J. Anim. Sci. 75:1773-1783.

Webel, D. M., B. N. Finck, D. H. Baker and R. W. Johnson. 1997. Time course of increased plasma cytokines, cortisol, and urea nitrogen in pigs following intraperitoneal injection of lipopolysaccharide. J. Anim. Sci. 75:1514-1520.

Guanglun Guo (1), Yulan Liu (1), *, Wei Fan (1), Jie Han (1), Yongqing Hou (1), Yulong Yin (1,2), Huiling Zhu (1) Binying Ding (1), Junxia Shi (1), Jing Lu (1), Huirong Wang (1), Jin Chao (1) and Yonghua Qu (1)

(1) Hubei Key Laboratory of Animal Nutrition and Feed Science, Wuhan Polytechnic University, Wuhan 430023, China

(2) Institute of Subtropical Agriculture, the Chinese Academy of Sciences, Changsha 410125, China.

* Corresponding Author: Y. L. Liu. Tel: +86-27-83956175, Fax: +86-27-83956175, E-mail: yulanflower@126.com
Table 1. Ingredient composition of the basal diet (as-fed basis)

Item %

Ingredient
 Corn 57.47
 Soybean meal (44% CP) 22.00
 Wheat middling 6.00
 Fish meal 6.00
 Soy oil 1.20
 Milk replacer 4.00
 Limestone 0.65
 Dicalcium phosphate 1.00
 Salt 0.31
 L-lysine x HCl (78.8% lysine) 0.32
 Butylated hydroquinone 0.05
 Vitamin and mineral premix (1) 1.00
Nutrient composition
 Digestible energy (2) (MJ/kg) 13.60
 Crude protein (3) 20.00
 Calcium (3) 0.80
 Total phosphorus (3) 0.70
 Total lysine (20 1.35
 Total methionine+cystine (2) 0.65

(1) Provided the following amounts of vitamins and trace minerals per
kilogram of complete diet: retinol acetate, 2,700 [micro]g;
cholecalciferol, 62.5 [micro]g; dl-[alpha]-tocopheryl acetate, 20 mg;
menadione, 3 mg; vitamin [B.sub.12], 18 [micro]g; riboflavin, 4 mg;
niacin, 40 mg; pantothenic acid, 15 mg; choline chloride, 400 mg;
folic acid, 700 [micro]g; thiamin, 1.5 mg; pyridoxine, 3 mg;
biotin, 100 [micro]g; Zn, 80 mg (ZnS[O.sub.4] x 7[H.sub.2]O);
Mn, 20 mg (MnS[O.sub.4] x 5[H.sub.2]O); Fe, 83 mg (FeS[O.sub.4] x
[H.sub.2]O); Cu, 25 mg (CuS[O.sub.4] x 5[H.sub.2]O); I, 0.48 mg
(KI); Se, 0.36 mg ([Na.sub.2]Se[O.sub.3] x 5[H.sub.2]O).

(2) Calculated. (3) Analyzed.

Table 2. Effect of feeding achyranthes bidentata polysaccharide
(ABPS) and lipopolysaccharide (LPS) challenge on performance of
weaned pigs (0 to 28 d) (a)

 -LPS +LPS

Item 0 mg of 500 mg of 0 mg of
 ABPS/kg ABPS/kg ABPS/kg
Average daily gain (g)
 0 to 14 d 212 212 203
 14 to 21 d (bc) 592 632 447
 21 to 28 d (de) 611 699 567
 Overall (b) 381 401 339
Average daily feed intake (g)
 0 to 14 d 330 353 349
 14 to 21 d (b) 779 788 664
 21 to 28 d (bc) 995 1,056 924
 Overall (b) 582 610 550
Feed:gain
 0 to 14 d 1.557 1.666 1.719
 14 to 21 d (b) 1.316 1.247 1.486
 21 to 28 d 1.628 1.579 1.631
 Overall (d) 1.527 1.522 1.622

 +LPS

Item 500 mg of SEM
 ABPS/kg
Average daily gain (g)
 0 to 14 d 207 23
 14 to 21 d (bc) 511 42
 21 to 28 d (de) 614 35
 Overall (b) 369 22
Average daily feed intake (g)
 0 to 14 d 335 18
 14 to 21 d (b) 729 52
 21 to 28 d (bc) 971 44
 Overall (b) 568 25
Feed:gain
 0 to 14 d 1.619 0.095
 14 to 21 d (b) 1.426 0.118
 21 to 28 d 1.582 0.092
 Overall (d) 1.539 0.046

(a) Lipopolysaccharide was injected on d 14 and 21. Values are means
(n = 6) for six pens per treatment with two pigs per pen.

(b) LPS effect (p<0.05). (c) Diet effect (p<0.10). (d) LPS effect
(p<0.10). (e) Diet effect (p<0.05).

Table 3. Effect of feeding achyranthes bidentata polysaccharide
(ABPS) and lipopolysaccharide (LPS) challenge on the proliferation of
lymphocytes isolated from peripheral blood in weaned pigs after both
lipopolysaccharide injection (a, b)

 -LPS

Item (c) 0 mg of 500 mg of
 ABPS/kg ABPS/kg
Following the first LPS injection
 LPS (8 [micro]g/ml (c)) 1.008 1.011
 ConA (8 [micro]g/ml (c)) 1.024 1.038
Following the second LPS injection
 LPS (8 [micro]g/ml (c)) 1.113 1.157
 ConA (8 [micro]g/ml) 1.148 1.102

 +LPS

Item (c) 0 mg of 500 mg of
 ABPS/kg ABPS/kg
Following the first LPS injection
 LPS (8 [micro]g/ml (c)) 1.045 1.099
 ConA (8 [micro]g/ml (c)) 1.166 1.121
Following the second LPS injection
 LPS (8 [micro]g/ml (c)) 1.398 1.355
 ConA (8 [micro]g/ml) 1.072 1.074

Item (c) SEM

Following the first LPS injection
 LPS (8 [micro]g/ml (c)) 0.031
 ConA (8 [micro]g/ml (c)) 0.050
Following the second LPS injection
 LPS (8 [micro]g/ml (c)) 0.112
 ConA (8 [micro]g/ml) 0.050

(a) Lipopolysaccharide was injected on d 14 and 21. Values are means
(n = 12) for all pigs per treatment. Values are expressed as
stimulation index, which is calculated as: absorbance of wells
incubated with concanavalin A (ConA) or LPS divided by the absorbance
of wells incubated without ConA or LPS.

(b) Blood was obtained 2 d after the first and the second LPS
administration. (c) LPS effect (p<0.05).

Table 4. Effect of feeding achyranthes bidentata polysaccharide (ABPS)
and lipopolysaccharide (LPS) challenge on serum immunoglobulin
G (IgG) level after both lipopolysaccharide challenges in
weaned pigs (a, b)

 -LPS +LPS

Item 0 mg of 500 mg of 0 mg of 500 mg of SEM
 ABPS/kg ABPS/kg ABPS/kg ABPS/kg
IgG (g/L)
 d 14 2.29 2.13 2.13 2.30 0.08
 d 21 2.22 2.26 2.34 2.35 0.11

(a) Lipopolysaccharide was injected on d 14 and 21. Values are
means (n = 12) for all pigs per treatment.

(b) Blood was obtained 3 h after the first and the second LPS
administration.

Table 5. Effect of feeding achyranthes bidentata polysaccharide (ABPS)
and lipopolysaccharide (LPS) challenge on plasma tumor necrosis
factor-[alpha] (TNF-[alpha]), prostaglandin [E.sub.2] (PG[E.sub.2]),
cortisol, insulin-like growth factor (IGF)-I and growth hormone (GH)
levels after both lipopolysaccharide challenges in weaned pigs (a, b)

 -LPS

Item 0 mg of 500 mg of
 ABPS/kg ABPS/kg

d 14
 TNF-[alpha] (pg/ml) 328 304
 PG[E.sub.2] (pg/ml) 671 694
 Cortisol (ng/ml) 51 71
 GH (ng/ml) 4.24 4.43
 IGF-I (ng/ml) 168 171
d 21
 TNF-[alpha] (pg/ml) 331 353
 PG[E.sub.2] (pg/ml) 554 580
 Cortisol (ng/ml) 67 81
 GH (ng/ml) 5.68 4.96
 IGF-I (ng/ml) 144 177

 +LPS

Item 0 mg of 500 mg of SEM
 ABPS/kg ABPS/kg

d 14
 TNF-[alpha] (pg/ml) 2,998 2,113 288
 PG[E.sub.2] (pg/ml) 1018 845 56
 Cortisol (ng/ml) 221 169 11
 GH (ng/ml) 5.12 4.62 0.66
 IGF-I (ng/ml) 108 152 16
d 21
 TNF-[alpha] (pg/ml) 2,468 1,767 225
 PG[E.sub.2] (pg/ml) 751 615 46
 Cortisol (ng/ml) 166 129 8
 GH (ng/ml) 4.90 5.42 0.60
 IGF-I (ng/ml) 129 137 10

 p-value

Item
 Diet LPS Interaction

d 14
 TNF-[alpha] (pg/ml) 0.031 <0.001 0.040
 PG[E.sub.2] (pg/ml) 0.062 <0.001 0.017
 Cortisol (ng/ml) 0.045 <0.001 <0.001
 GH (ng/ml) 0.736 0.258 0.465
 IGF-I (ng/ml) 0.042 0.001 0.075
d 21
 TNF-[alpha] (pg/ml) 0.039 <0.001 0.028
 PG[E.sub.2] (pg/ml) 0.096 0.001 0.015
 Cortisol (ng/ml) 0.059 <0.001 <0.001
 GH (ng/ml) 0.809 0.711 0.149
 IGF-I (ng/ml) 0.004 <0.001 0.079

(a) Lipopolysaccharide was injected on d 14 and 21. Values are
means (n = 12) for all pigs per treatment.

(b) Blood was obtained 3 h after the first and the second LPS
administration.
COPYRIGHT 2008 Asian - Australasian Association of Animal Production Societies
No portion of this article can be reproduced without the express written permission from the copyright holder.
Copyright 2008 Gale, Cengage Learning. All rights reserved.

Article Details
Printer friendly Cite/link Email Feedback
Author:Guo, Guanglun; Liu, Yulan; Fan, Wei; Han, Jie; Hou, Yongqing; Yin, Yulong; Zhu, Huiling; Ding, Binyi
Publication:Asian - Australasian Journal of Animal Sciences
Article Type:Report
Geographic Code:9CHIN
Date:Aug 1, 2008
Words:6048
Previous Article:Effects of egg storage material and storage period on hatchability in Japanese quail.
Next Article:Probiotics in drinking water alleviate stress of induced molting in feed-deprived laying hens.
Topics:


Related Articles
Effects of organic acids on growth performance, gastrointestinal pH, intestinal microbial populations and immune responses of weaned pigs.
Effects of [beta]-glucan from Paenibacillus polymyxa and L-theanine on growth performance and immunomodulation in weanling piglets.
Effects of dietary zinc level and an inflammatory challenge on performance and immune response of weanling pigs.
Dietary L-arginine supplementation improves intestinal function in weaned pigs after an Escherichia coli lipopolysaccharide challenge.

Terms of use | Privacy policy | Copyright © 2020 Farlex, Inc. | Feedback | For webmasters