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Effect of partial replacement of concentrates with Barhar (Artocarpus lakocha) leaves on growth performance of kids fed a mixed jungle grass-based diet.


Most of the goats in mid-altitude regions of the Himalaya are raised by poor and landless farmers. They cut and carry vegetation from the backyard, farm boundaries, community land and nearby forest land. Such vegetation comprises many different kinds of plant species and leaf blades and is grossly termed as "mixed jungle grass". As forest grazing is not desirable in this fragile ecosystem of the state, mixed jungle grass plays a pivotal role in raising goats. Earlier, Balaraman and Gupta (1990) suggested that mixed jungle grass could support only maintenance of goats. It is therefore desirable to increase intake and digestion of nutrients and thereby growth performance of kids through supplementation. Supplementation with concentrate increased production performance of sheep (Karim et al., 2001) and goats (Das and Ghosh, 2000; Das and Ghosh, 2001a) reared under grazing on native pasture. However, considering the cost and availability of concentrates, it will be wise to use it most judiciously and to replace it with locally available feed ingredients like tree fodders. However, there are only few reports concerning direct replacement of concentrates with tree fodders. These studies indicate that responses to supplementation vary with the nature of the basal diet (Mui et al., 2002), degradation characteristic of the forage (Bates et al., 1988), level of replacement and interaction between nutrients of the feed ingredients (Brown and Pitman, 1991).

Barhar (Artocarpus heterophyllus) is one of the most popularly grown trees in the Himalayan mid-altitude region. The tree is grown as a component of traditional Silvi-pasture system (Singh et al., 1996) in the Himalayan region. The crown volume of a fully-grown tree may go up to 246 sqm, with biomass yields of 102 kg DM/tree. The trees are lopped and fed to livestock during the winter period when the scarcity of fodder is very acute. Barhar leaves are very highly palatable and contain 8% digestible CP and 59% TDN (Das and De, 2001), and like jack fruit leaves have the potential to partially replace concentrates (Das and Ghosh, 2007). This experiment was conducted to study the effect of partial replacement of concentrates with Barhar leaves on growth performance of kids fed a mixed jungle grass-based diet.


Experimental site

The experiment was conducted at the ICAR Research Complex for the North Eastern Hill Region, Sikkim Centre, Tadong, situated in the East District of Sikkim State in the Northeast region of India, at an elevation of 1,325 msl. The general topography of the site is hilly. The climate of the area is sub-tropical humid with distinct seasonal variation. During the trial period (November to February) the site recorded 212.4 mm of rainfall and the average temperature ranged from 7.2 to 22.9[degrees]C.

Animals and design of experiment

Fifteen male Sikkim local kids, about 4 months of age and body weight ranging from 5.8 to 9.2 kg, were selected. Before onset of the experiment, the animals were washed with 2 ml of delta methrin (Butox, Inter Vet India Ltd, Bombay) diluted in 1 litre. They were also drenched with Albomar (Analgon, Wockhardt Veterinary Pvt. Ltd., Bombay) at 7.5 mg/kg BW. The animals were randomly distributed into one of the 3 dietary treatments.

Herbal composition of mixed jungle grass

For determination of the herbal composition of mixed jungle grass, 5 plots of 4 sqm each were randomly selected during the digestion trial. All the vegetation was removed at ground level and weighed. A sample (1 kg) was separated into the different components. Mixed jungle grass of Sikkim is comprised of various species of grasses, shrubs and weeds. The components of the mixed jungle grass were identified by local names and then correlated with the literature for their respective botanical name. During the experimental period most of the herbage components were at a late stage of maturity. The predominant pasture species were Setaria palmifolia (25.1%), Pennisetum purpureum (21.3%), Thysaenolena maxima (11.4%), Penicum maxima (5.2%), Ageritum conyzoides (9.1%), Euopotaruim odoratum (8.9%), Desmodium intortum (13.05) and Crystellina parasitica (3.0%). The rest (4.1%) of the mixed jungle grass was comprised of leaf blades and stems from unidentified species.

Feeding management and dietary treatments

The kids were housed in a well-ventilated shed with arrangements for individual feeding and faeces collection. A concentrate mixture (maize 35, rice bran 30, mustard cake 32, common salt 1, mineral mixture 2 kg) was offered at 7.00 am. Immediately after consumption of the concentrate, mixed jungle grass was offered and made available to the kids for the rest of the day. Animals in group I received concentrate at 2% of their body weight; 25 and 50% of the concentrate was replaced with an equal amount (DM basis) of Barhar leaves in groups II and III, respectively. After lopping, Barhar leaves were separated from branches and offered to the animals at 2.00 pm. Clean and fresh drinking water was freely available to the kids.

Estimation of intake and digestibility, collection of blood and rumen fluid

During the mid-phase of the trial (days 50-56), the animals were transferred to metabolism cages. After an adaptation period of 7 days, a digestion trial of 5 days was conducted. Blood was collected from the jugular vein with a hypodermic needle 2 h prior to feeding on days 30, 60 and 89 of the study. Blood glucose was analyzed on the same day. Another sample of blood was used to harvest plasma using heparin as anticoagulant. Rumen liquor was collected from each kid using a stomach tube at 0, 2, 4, 6 and 8 h after feeding on day 31, 61 and 90 of the study.

In sacco-degradation characteristics

For rumen degradation studies, fortnightly samples of Barhar leaves, pasture herbage and concentrates were collected up to 56 days of the trial and pooled. Samples were ground (2 mm sieve) and about 3 g of samples were put into nylon bags (60 mx12 cm, 41 pore size) and incubated inside the rumen of three ruminally-fistulated steers fed a diet of maize fodder and concentrate mixture. Samples of mixed jungle grass and Barhar leaves were incubated for 6, 12, 24, 48, 72 and 96 h; concentrate mixture was incubated for 3, 6, 9, 12, 18 and 24 h. On removal of the bags after each incubation period, the bags were rinsed under tap water until the wash water was clean and then the bags were oven dried at 60[degrees]C for 48 h for determination of DM. Residues remaining in the bags were analyzed for N (AOAC, 1984).

Chemical analysis

Estimation of tannins in mixed jungle grass and Barhar leaves : Condensed and other fractions of tannins in jungle grasses and Barhar leaves were estimated as per the method described by Makkar (2003). Leaf samples (200 mg) were extracted with ultrasonicator (Cell Disrupter Ultrasonic Probe, Model 1000L) at 4[degrees]C in a 10 ml aqueous acetone solution (acetone/water: 7/3 v/v). After centrifugation (3,000xg at 4[degrees]C for 20 min), the supernatants (total phenolics extract) were analyzed for phenolic components (total phenolics, non-tannin phenolics, total tannin phenolics and condensed tannins) as described by Makkar (2003).

Contents of total phenolics was analyzed using Folin-Ciocalteu reagent (Sisco Research Laboratories Pvt., Ltd, Mumbai, India) based on tannic acid standard (Qualigens fine chemicals, GlaxoSmithKline Pharmaceuticals Ltd., Mumbai, India). Total phenolics consist of simple phenolic compounds or non-tannin phenolics and pure tannins or total tannin phenolics. Polyvinyl polypyrrolidone (PVPP, Sigma-Aldrich) has the property to bind tannins but not the simple phenolics. Distilled (triple glass) water (2 ml) and total phenolics extract (2 ml) were added to the test tube containing 200 mg PVPP, vortexed twice and filtered through Whatman No. 1 filter paper. The filtrate was used to estimate non-tannin phenolics, which was subtracted from total phenolics to obtain total tannins. The concentration of total phenolics and total tannins were expressed as tannic acid equivalent.

Condensed tannin : Three ml n-butanol-HCl (95:5 v/v) and 0.1 ml ferric ammonium sulphate (1%) were added to the test tube containing 0.5 ml phenolics extract. The test tube was closed with a glass marble and heated in a boiling water bath for 60 min. The absorbance of the red anthocyanidin products (i.e. condensed tannin) was measured at 550 nm and condensed tannin was expressed as leucocyanidin equivalent.

Proximate principles and cell wall components : Forage and fecal samples were analyzed for DM, CP, ash and acid insoluble ash (AOAC, 1984), NDF, ADF and acid detergent lignin (ADL) (Van Soest et al., 1991). Samples incubated in nylon bags and their residues were analyzed for DM and N. Frozen fecal samples were analyzed for DM, N and NDF. Hemicellulose was calculated as the difference between NDF and ADF. Similarly, cellulose was calculated as the difference between ADF and ADL.

Rumen and blood metabolites : Ammonia-N in rumen liquor was estimated by the micro-diffusion technique of Conway as cited by Raghuramulu et al. (1983). TVFA concentration in rumen fluid was measured by Markham's distillation (Barnett and Reid, 1957). A digital pH meter was used to measure pH of rumen fluid. Serum samples were analyzed for glucose (Folin and Wu, 1920), total protein (Lowry et al., 1951), albumin (Doumas et al., 1971) and urea (Rahamatullah and Boyde, 1980); hemoglobin in whole blood was estimated as described by Dacie and Lewis (1968).

Estimation of minerals in feed and serum samples : Estimation of Ca and P in feed samples was done by the methods of Talapatra (1940) and AOAC (1984), respectively. Serum Ca and P were determined using a diagnostics kit (Span Diagnostics Ltd., Sachin, India). Concentrations of Fe, Zn and Cu in feed and serum samples were determined using an atomic absorption spectrophotometer (Model No: AAS 4141, ECIL, Hyderabad, India).

Calculation and statistics

To characterize the rumen degradability of feeds, their rumen degradation values were fitted to the equation:

Y = a + b (1 - [e.sup.-ct])

Where Y is the degradation at time t and a, b, c are parameters describing rapidly and slowly degradable fractions and rate of degradation, respectively (Orskov and McDonald, 1979). Data obtained were analyzed statistically following the procedure for a randomized block design, and treatment means were separated using students "t" test (Snedecor and Cochran, 1967).


Chemical composition of feeds and fodders

Chemical composition of feeds and fodder and their degradation characteristics are presented in Table 1. Proportion of N bound to NDF was greater in Barhar leaves in comparison to pasture and concentrate. Rate and extent of degradation of DM and N was much greater in concentrate in comparison to pasture and Barhar leaves. Rapidly soluble and potentially degradable fraction of both DM and N was greater in pasture in comparison to Barhar leaves. Mixed jungle grass contained much higher condensed tannins than most of the cultivated grasses/grass based diets. Total condensed tannin of Barhar leaves, however, was 9 times higher than that of mixed jungle grass. Both mixed jungle grass and Barhar leaves were characterized by high Ca and low P level. Concentration of Zn and Cu was within normal range for both the feed ingredients; however, they were characterized by higher Fe content.

Chemical composition of components of mixed jungle grass

The mixed jungle grass comprised 4 grass species (Setaria palmifolia, Panicum maximum, Thysanolaena maxima, and Pennisetum polystachyon), 1 legume (Desmodium intortum), 1 member of compositae (Eupotarium odoratum), 1 member from Asteracecae (Ageritum conyzoides) and 1 fern (Crystellina parasiticus). Desmodium intortum contained the most N and least NDF, followed by Setaria palmifolia (Table 2). Setaria palmifolia contained the least amount of condensed tannins. Ageritum conyzoides and Eupotarim oduratum were the most unique component of the mixed jungle grass. There is no previous report concerning their nutritional or anti-nutritional value. Nevertheless, they contained higher amounts of condensed tannins and, with Desmodim intortum, contributed to the higher condensed tannins content of mixed jungle grass (Table 3).

Feed consumption, nutrient intake and diet digestibility

Feed and nutrient intake are presented in Table 4. Intake of DM, OM, NDF, ADF, hemicellulose and cellulose was not significantly different among the groups. Replacing concentrates with Barhar leaves resulted in a small, but significant decrease (p<0.05) in CP intake. Overall tract digestibility of DM and OM was not significantly different among the groups (Table 4). However, increasing Barhar leaves in the diet resulted in an increase (p<0.01) in digestibility of NDF, ADF (p<0.05), hemicelluloses (p<0.05) and cellulose (p<0.01), whereas digestibility of N decreased (p<0.01).

Rumen fermentation, blood metabolites and diet digestibility

Ruminal pH and TVFA concentration were not significantly different among the groups however, rumen ammonia-N concentration decreased (p<0.01) with increased level of Barhar leaves in the diet. Blood urea N and blood glucose levels were lower (p<0.05) in groups fed Barhar leaves (Table 5).

Intake and serum profile of certain minerals

Intake and serum profile of selected minerals are presented in Table 6. Intake of P decreased (p<0.05) as the level of Barhar leaves increased in the diet. Intakes of other minerals were not significantly different among the groups. Replacement of 50% of the concentrates with Barhar leaves resulted in decreased blood hemoglobin (p<0.01) and serum Fe (p<0.05) concentration.

Growth performance

Replacing 25% of concentrate with Barhar leaves showed no effect on ADG. However, replacing 50% of the concentrate resulted in reduction (p<0.05) in ADG (Table 7). Replacing 50% of the concentrates with Barhar leaves resulted in increased (p<0.05) fecal excretion and decreased (p<0.05) N balance.


Herbal and chemical composition of feeds and fodder

Herbal composition of the mixed jungle grass during the trial period was quite different from that observed during the monsoon at the same location (Balaraman and Gupta, 1990). However, the herbal composition of mixed jungle grass reported from the same location during winter (Das, 2005) was more or less similar to that observed during the present investigation. CP content of mixed jungle grass during winter (present experiment) was lower and NDF content was higher than the values reported earlier during the monsoon from the same location (Balaraman and Gupta, 1990). Thus, it is evident that herbal and chemical composition of mixed jungle grass varies according to the season. Similar change in nutrient composition was also reported in arid pasture (Shinde et al., 1998). The change in nutrient composition could be correlated with stage of maturity. During the monsoon, most of the pasture components were in pre-flowering/full bloom stage, during which the nutrient concentration is maximum.

Among the different components of mixed jungle grass, Setaria palmifolia was characterized by higher CP, lower NDF and very low content of tannins. These attributes make it a suitable component for a native silvi-pasture system (Rai et al., 1988). Another notable component of mixed jungle grass was Thysanolaena maxima, the broom grass. Usually this multipurpose herb is grown in the terrace risers for conservation of soil, flowers are used as commercial broom and, after harvesting of broom, the leaves are fed to livestock and the left-over sticks are either used as fuel or for thatching. The broom grass is often grown wild in the scrub and forest land. Two of the most striking components of mixed jungle grass were Ageritum conyziodes and Eupotarium odoratum. There is no previous report regarding their nutritional/anti-nutritional value. Nevertheless they were characterized by a high content of tannins and, along with Desmodium intortum, probably contributed to the high tannin content of mixed jungle grass. It seems that C3 plants accumulate more condensed tannins than C-4 plants (Capinera et al., 2005).

The chemical composition of Barhar was similar to earlier reports on the same species (Das and De, 2001; Khanal and Subba, 2001). The leaves contained much higher tannins and condensed tannins than other tree fodder of the region (Khanal and Subba, 2001). Another notable feature of the leaves was the very low content of P. The P content of Barhar was 3 times lower than that of the concentrate mixture. Thus, extensive replacement of concentrates with Barhar leaves may impair P metabolism and animal performance.

Rate and extent of degradation of Barhar leaves was similar to pasture but lower than concentrates. This is contrary to leguminous tree fodders, which are very highly degradable (Bonsi et al., 1995). Such difference could be due to more NDF and ADF, and also due to more proportion of ADF-N and NDF-N in Barhar leaves. About 48 and 41% of N of Barhar leaves was bound to NDF and ADF, respectively. ADF-bound N is unavailable for degradation as observed in the case of Artocarpus heterophyllus (Das and Ghosh, 2007).

Nutrient intake, rumen fermentation pattern, blood metabolites and diet digestibility

As the goats consumed the entire amount of Barhar leaves offered to them, replacing concentrates with Barhar leaves did not affect the total DMI. Similarly, replacing concentrates with Gliricidia leaves showed no affect on total DMI in growing and lactating goats (Richards et al., 1994a, b). However, replacing concentrate with Sesbania leaves reduced DMI in lactating cows (Khalili and Varvikko, 1992). This difference in response could be attributed largely to the reluctance of animals to eat all the leaves and also due to wilting of Sesbania leaves, which resulted in dry hard stems which were most probably unpleasant to eat. A. lakocha, leaves were, however, highly palatable and the animals consumed the entire amount offered to them (Das and De, 2001). Replacing concentrates with tree fodder like Artocapus heterophyllus resulted in increased pasture intake (Das and Ghosh, 2007). Failure to observe any such positive response in the present experiment could be explained on the basis of a very high level of intake in the control group. It is to be noted that in this experiment, DMI as a proportion of BW was higher than 6.00% in all the groups. However, DMI in goats fed solely on Jack fruit leaves was only 4.11% (Das and Ghosh, 2001b), whereas it was 4.61% of BW in goats fed solely on Barhar leaves (Das and De, 2001). It was amazing to note that a diet comprising of components like Ageritum conyzoides, Eupotarium odoratum and Crystellina parasitica (all of which contained high level of condensed tannins and their nutritional/anti-nutritional attributes require further elaborate study) was relished to such an extent by the goats. Opportunistic feeding behavior of goats and their preference for a greater variety of feeds (Sharma et al., 1998) over a large quantity of one type of feed might have resulted in increased DMI in goats fed on a jungle grass-based diet. Overall DMI up to 6.71% of BW has been recorded in Black Bengal goats fed on a Jack fruit leaves-based diet supplemented with concentrates (Kibria et al., 1994). Mackenzie (1970) has indicated that, in terms of DM consumption, records up to 15% of BW have been observed in goats compared to only 2.5-3.0% in the case of cattle and sheep.

Rumen pH was not significantly different among the groups. The pH was above the cellulolytic threshold of 6.2 (Mould and Orskov, 1983) in all groups. Rumen N[H.sub.3]-N concentration decreased (p<0.01) with increasing level of replacement. This was in accordance with lower degradability of N from Barhar leaves. Similar observations were made when the concentrate mixture was replaced with tree fodders like Sesbania (Khalili and Varvikko, 1992) and Gliricidia (Richards et al., 1994a). However, the N[H.sub.3]-N level was above or about 100 mg/L in all the groups, which has been recommended as the minimum acceptable level for optimization of digestion of a forage-based diet (Leng, 1990; Das and Singh, 1999). In this experiment, feed consumption and overall tract digestibility was similar in all the groups, resulting in similar DOM intake. This effect was also reflected in similar TVFA concentration in all the groups. Replacement of concentrates with jack fruit leaves in previous studies produced similar responses (Das and Ghosh, 2007).

Blood urea concentration was a reflection of the rumen N[H.sub.3]-N level. This could be due to a decrease in protein degradability. Similar responses have been observed when concentrates were replaced with jack fruit leaves (Das and Ghosh, 2007). Replacing concentrate with Barhar leaves resulted in a decreased blood glucose level. This could be due to the increased acetate and decreased propionate observed with increasing Barhar leaves in the diet. Replacement of concentrates with tree leaves resulted in a higher proportion of fibre in the diet. In a diet containing more fibre, acetate production increases at the expense of propionate owing to low carbon flow through electron accepting channels such as the glycolytic acid-propionate production pathway (Van Houlert, 1983). Replacing a concentrate mixture with jack fruit leaves, produced a similar response in earlier studies (Das and Ghosh, 2007).

In spite of the slower rate and extent of degradation of Barhar leaves in comparison to concentrate, digestibility of DM and OM was similar in all groups. However, digestibility of NDF increased and that of CP decreased with increased level of Barhar leaves in the diet. Similar observations have been reported when concentrate mixture was replaced with Sesbania in lactating cows (Khalili and Varvikko, 1992), Gliricidia in growing goats (Richards et al., 1994a) or Artocarpus heterophyllus in kids (Das and Ghosh, 2007). Increased fibre digestibility in the group fed Barhar leaves could be due to supply of degradable cellulose by Barhar leaves which is essential to seed fibrolytic bacteria (Silva and Orskov, 1988). The concentrate-fed group, although provided with readily degradable nitrogen, contained very negligible amounts of degradable cellulose and hemicelluloses. Replacing concentrates with Barhar leaves is expected to decrease the passage rate and increase mean retention time due to their bulkiness (Bonsi et al., 1994). Higher levels of concentrate in the control diet might have increased the rate of passage of particulate matter from the reticulo-rumen, which is inversely correlated to digestibility (Aitchison et al., 1985). It is logical to assume that replacing concentrates with Barhar leaves would result in decreased level of starch in the rumen. Consequently, the adverse effect of starch on fibre digestion was lower when concentrate was replaced with Barhar leaves. As a result, fibre digestion was higher in groups fed Barhar leaves.

Intake and serum profile of some minerals

Mixed jungle grass and Barhar leaves had a higher Ca content, more than 1% on DM basis, which made these feed ingredients suitable for early lactation and growth. The P content of both mixed jungle grass and Barhar leaves was relatively low, but within the normal requirement range (Khanal and Subba, 2001). As the concentration of Ca was higher and P was lower in mixed jungle grass, the cereal byproduct-based concentrate mixture which was rich in P and low in Ca was a perfect supplement for the mixed jungle grass-based diet. Replacing concentrates with tree leaves, however, may upset this balance and may cause some problems with Ca, P and Vitamin D metabolism (Khanal and Subba, 2001). Replacing 50% of the concentrate with Barhar leaves resulted in a 22% reduction of P intake. Considering the large variation in P content between concentrates and Barhar leaves, the extent of reduction in P intake was small, because consumption of Barhar leaves never exceeded 20% of dietary DM. Such difference in intake of P was, however, not reflected in serum P level. At a marginal level of lower intake, input of P to the blood pool is maintained by an increased bone resorption and P mobilization from soft tissues. As all the animals were growing in the present experiments, mobilization of P from bone and soft tissue is least likely. Rather, the efficiency of absorption might have increased in the low intake group due to active demand for P as observed by Vitti et al. (2000). Further, P intake was much higher than the recommended value of 0.91 g/d for a 20 kg goat growing at 50 g/d (AFRC, Kessler, 1991). Hence, replacement of concentrates with Barhar leaves is not likely to hamper production performance of goats through interference in P metabolism.

Both mixed jungle grass and Barhar leaves contained Zn and Cu above the normal requirement range (Khanal and Subba, 2001). It is notable that both feed ingredients contained Fe above its normal range. High levels of Fe could mostly be attributed to the acidic soils of Sikkim since such soils may elevate Fe levels in plants (McDowell, 1997). In spite of higher Fe intake, replacing concentrates with Barhar leaves resulted in decreased blood hemoglobin and serum Fe concentration. Priolo et al. (2000) reported that even a small amount of condensed tannin may depress blood Hb concentration. Tannins are known to chelate Fe and thus reduce absorption (Gillooly et al., 1983). The lower hemoglobin concentrations of animals fed a higher level of Barhar leaves are probably a direct result of this effect. There are conflicting results on the effect of tannins on availability of Cu and Zn (Jansman et al., 1993; Vaquero et al., 1994). In this experiment, irrespective of dietary level of Barhar leaves and condensed tannins, serum Cu and Zn levels were similar in all the groups.

Nitrogen utilization and growth performance

CP digestibility decreased with increased level of Barhar leaves in the diet. Similarly, decreased N utilization has been reported when a concentrate mixture was replaced with tree fodders like Sesbania (Khalili and Varvikko, 1992) or Gliricidia leaves (Richards et al., 1994a). This could be due to slower rate and extent of degradation of N from Barhar leaves as most of its N is bound to the fibre fraction as already discussed. Greater faecal N production in goats fed Artocarpus lakocha leaves suggests impairment of N utilization in the small intestine. Traditionally, impairment of N utilization in tree fodder-based diets has been attributed to the presence of tannins and other phenolics (Bakshi and Wadhawa, 2004). In this experiment, the total tannin content was 15.8% of DM, which is much higher than reported in other tree fodders of the region (Khanal and Subba, 2001). Replacing concentrate with Barhar leaves resulted in a linear increase in the condensed tannin content of the diet. Even though goats are more tolerant to tannin content than sheep and other ruminants (Narjisse et al., 1995), impairment of N utilization was observed in the present experiment when concentration of condensed tannins was 3.70%. In fact, even a small amount of dietary condensed tannin may adversely affect the performance of animals (Priole et al., 2000).

In this experiment, replacing 50% of concentrates with Barhar leaves resulted in reduced ADG. Reduced protein intake or amino acid absorption in the small intestine most likely limited performance rather than energy. Blood glucose level in group III was lower than in other groups, which indicates that propionate level was lower in that group. It would be logical to assume that supply of glucogenic amino acids for protein synthesis was lower in group III due to lower availability of propionate. However, these adverse effects on protein intake and utilization were not evident when the replacement was restricted to 25%. Protein intake and utilization were similar in groups I and II. It seems that Barhar leaves can replace 25% of the concentrates.


Although there was large variation in CP content of concentrate and Barhar leaves, CP consumption was similar in groups I and II, and there was only a small decrease in CP consumption in group III in comparison to the control. On the contrary, there was large variation in CP digestibility among the groups. The CP digestibility decreased by 9 and 17% in groups II and III, respectively. It was evident that most of the adverse effects of replacing 50% of the concentrates with Barhar (Artocapus lakocha) leaves were due to impairment of utilization of protein rather than its intake.


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A. Das *, D. De and S. Katole (1)

ICAR Research Complex for NEH Region, Sikkim Centre, Tadong-737 102, Sikkim, India

* Corresponding Author: A. Das. Centre for Wildlife, Indian Veterinary Research Institute, Izatnagar- 243 122 UP, India. Tel: +91-9412048518, Fax: +91-581-2303286, E-mail: asit@ivri.

(1) Indian Veterinary Research Institute, Izatnagar- 243 122 UP, India.

Received January 20, 2010; Accepted May 11, 2010
Table 1. Chemical composition of feeds and fodders used for the
digestion trial

Parameter                               Mixed jungle grass (1)

DM (g/kg)                                    215.1
(g/kg DM)
  Organic matter                             883.2
  Crude protein                               68.7
  NDF                                        678.9
  ADF                                        424.5
  Hemicellulose                              254.4
  Cellulose                                  289.5
  Nitrogen                                    10.99
  NDF-nitrogen (%)                            44.98

Concentration of minerals
  Ca (g/kg)                                   13.7
  P (g/kg)                                     3.5
  Cu (mg/kg)                                   8.6
  Fe (mg/kg)                                   5.9
  Zn (mg/kg)                                  56
  Mn (mg/kg)                                  44

Concentration of phenolics (g/kg DM)
  Total phenolics                             44
  Non-tannin phenolics                        18
  Tannin phenolics                            26
  Condensed tannins                           18

Degradation characteristics (%)
    a                                         28.33+0.55
    b                                         35.06+0.73
    c (%/h)                                    3.74+0.14
    a                                         36.65+0.94
    b                                         42.56+1.36
    c (%/h)                                    5.03+0.14

Parameter                               Barhar (2)    Concentrates (3)

DM (g/kg)                               338.8           894.2
(g/kg DM)
  Organic matter                        894.5           904.2
  Crude protein                         123.9           160.45
  NDF                                   321.6           123.6
  ADF                                   252.0            52.4
  Hemicellulose                          69.6            71.2
  Cellulose                             136.1            26.2
  Nitrogen                               20.6            31.8
  NDF-nitrogen (%)                       48.45            7.71

Concentration of minerals
  Ca (g/kg)                              15.2             4.0
  P (g/kg)                                3.9            12.4
  Cu (mg/kg)                              9.5            10.2
  Fe (mg/kg)                            577             312
  Zn (mg/kg)                             43              65
  Mn (mg/kg)                             62              70

Concentration of phenolics (g/kg DM)
  Total phenolics                       204                  nd
  Non-tannin phenolics                   11                  nd
  Tannin phenolics                      193                  nd
  Condensed tannins                     158                  nd

Degradation characteristics (%)
    a                                    25.15+0.79      43.08+0.99
    b                                    32.11+2.62      51.29+0.50
    c (%/h)                               3.48+0.04       8.95+0.05
    a                                    29.65+1.44      47.43+1.36
    b                                    36.70+1.11      49.59+0.33
    c (%/h)                               4.69+0.29      11.06+0.20

a = Rapidly degradable fraction, b = Slowly degradable fraction,
c = Rate of degradation.

(1) Late stage of maturity. (2) Mature leaves.
(3) Maize 35%, mustard cake 32%, rice bran 30%, mineral mixture 2%
and common salt 1%.

Table 2. Chemical composition of pasture herbage

                                         Chemical composition
                                             (g/kg DM)

                                      OM     N      NDF     ADF

Setaria palmifolia        252   243   893   11.9   654.5   297.3
Pennisetum polystachyon   213   292   906   11.5   742.5   432.2
Thysanolaena maxima       114   328   882    6.7   782.5   462.9
Panicum maxima             52   268   912    9.3   741.9   392.5
Ageritum conyzoides        91   312   864   10.9   664.5   582.5
Euopotaruim odoratum       89         876   18.4   692.3   612.6
Desmodium intortum        130   287   902   34.4   582.3   504.5
Crystellina parasitica     30   179   842   13.4   496.7   372.6
Others                     41

                          Chemical composition (g/kg DM)

                           Hemicellulose      Cellulose

Setaria palmifolia             357.2            252.5
Pennisetum polystachyon        310.3            364.8
Thysanolaena maxima            319.6            342.8
Panicum maxima                 349.4            332.7
Ageritum conyzoides             82.0            504.5
Euopotaruim odoratum            79.7            432.5
Desmodium intortum              77.8            362.5
Crystellina parasitica         124.1            301.2

Table 3. Phenolics concentration of different components of mixed
jungle grass

                                   Phenolics (g/kg DM)

                          Total phenolics    Non-tannin phenolics

Setaria palmifolia               10                    7
Pennisetum polystachyon          42                   18
Thysanolaena maxima              29                   14
Panicum maxima                   29                    9
Ageritum conyzoides              85                   14
Euopotaruim odoratum             67                   15
Desmodium intortum              125                   21
Crystellina parasitica           37                    8

                                 Phenolics (g/kg DM)

                          Tannin phenolics    Condensed tannins

Setaria palmifolia                3                    2
Pennisetum polystachyon          24                   11
Thysanolaena maxima              15                   13
Panicum maxima                   20                   12
Ageritum conyzoides              70                   56
Euopotaruim odoratum             52                   34
Desmodium intortum              104                   87
Crystellina parasitica           29                   19

Table 4. Effect of partial replacement of concentrate mixture with
Barhar on feed consumption, nutrient intake and digestibility in
Sikkim local kids fed a mixed jungle grass-based diet

                                      Dietary treatments (1)

Parameter                                I            II

Feed consumption (g/d)
  Mixed jungle grass                 421.00       406.00
  Concentrate **                     178.84 (a)   134.13 (b)
  Artocarpus lakocha                     --        50.82
  Total                              599.84        589.95
As % of body weight
  Nutrient intake (g/d)
    OM                               533.52       525.18
    CP *                              58.34 (a)    56.21 (ab)
    NDF                              329.38       333.11
    ADF                              211.10       215.36
    Hemicellulose                    118.27       117.75
    Cellulose                        152.49       153.40
  Digestibility of nutrients (%)
    DM                                63.38        66.17
    OM                                64.38        67.44
    CP **                             71.57 (a)    65.48 (b)
    NDF *                             48.66 (a)    53.25 (b)
    ADF *                             47.22 (a)    52.08 (b)
    Hemicellulose *                   51.21 (a)    55.65 (b)
    Cellulose **                      49.83 (a)    56.75 (b)

                                     treatments (1)

Parameter                                   III        SEM

Feed consumption (g/d)
  Mixed jungle grass                   414.00          0.74
  Concentrate **                        89.42 (c)      0.81
  Artocarpus lakocha                    92.13          0.82
  Total                                595.54          0.73
As % of body weight
  Nutrient intake (g/d)
    OM                                 528.89         19.15
    CP *                                54.21 (b)      0.21
    NDF                                347.51          0.61
    ADF                                226.70          8.51
    Hemicellulose                      120.81          4.66
    Cellulose                          158.41          5.65
  Digestibility of nutrients (%)
    DM                                  64.30          0.24
    OM                                  65.30          1.93
    CP **                               59.60 (c)      0.24
    NDF *                               53.30 (b)      0.24
    ADF *                               51.29 (b)      1.43
    Hemicellulose *                     56.96 (b)      1.47
    Cellulose **                        55.00 (b)      1.13

(a,b,c) Means in the same line with different letters differ
significantly, * p<0.05, ** p<0.01. SEM = Standard error of the mean.

(1) All the kids were fed mixed jungle grass ad libitum. Kids of Group
I received concentrate at 2% of BW; however, 25 and 50% of the
concentrate was replaced with equal amount (DM basis) of Barhar leaves
in groups II and III, respectively.

Table 5. Effect of partial replacement of concentrates with Barhar
leaves on feed consumption, nutrient utilization, rumen fermentation
pattern and certain blood metabolites in Sikkim local goats fed a
mixed jungle grass-based diet

                            Dietary treatments (1)

                         I            II          III        SEM

Rumen fermentation
  pH                   6.85         6.91         6.89       0.044
  [NH.sub.3] **      198.6 (c)    155.6 (b)    115.4 (a)    0.788
  VFA                112          107.8        106          0.293
Blood metabolites
  Hb (g/dl) **        14.08 (a)    13.06 (a)    11.22 (b)   0.59
  Glucose **          61.80 (c)    57.40 (b)    50.00 (a)   0.30
  BUN *               20 (b)       14 (a)       12 (a)      0.256
  Protein              6.68         6.86         6.84       0.062
  Albumin              3.42         3.36         3.58       0.061
  Globulin             3.18         3.50         3.16       0.074

(a,b,c) Values bearing different superscript in a row differ
significantly, * p<0.05 ** p<0.01.

SEM = Standard error of the mean. (1) For detail see Table 4.

Table 6. Effect of partial replacement of concentrates with Barhar
leaves on intake and serum mineral profile of Sikkim local goats
fed a mixed jungle grass-based diet

                                Dietary treatments (1)

                           I              II            III

  Ca (g/dl)               6.48           6.87           7.44
  P (g/dl) **             3.70 (a)       3.28 (b)       2.92 (c)
  Fe ([micro]g/L)       305.03         311.52         326.14
  Zn ([micro]g/L)        35.36          33.79          33.11
  Cu ([micro]g/L)         5.44           5.34           5.35
Serum profile
  Ca (g/dl)              10.38          10.68          10.80
  P (g/dl)                4.82           4.84           4.84
  Fe ([micro]g/L) *   1,520 (a)      1,428 (ab)     1,310 (b)
  Cu ([micro]g/L)     1,216          1,206          1,150
  Zn ([micro]g/L)       590            578            568


  Ca (g/dl)            0.27
  P (g/dl) **          0.067
  Fe ([micro]g/L)     11.51
  Zn ([micro]g/L)      0.87
  Cu ([micro]g/L)      0.17
Serum profile
  Ca (g/dl)            0.45
  P (g/dl)             0.16
  Fe ([micro]g/L) *   42.23
  Cu ([micro]g/L)     16.04
  Zn ([micro]g/L)     15.55

(a,b,c) Values bearing different superscript in a row differ
significantly, * p<0.05, ** p<0.01.

SEM = Standard error of the mean. (1) For detail see Table 4.

Table 7. Effect of partial replacement of concentrates with
Artocapus lakocha leaves on N balance and growth performance
of Sikkim local goats fed a mixed jungle grass-based diet

                           Dietary treatments (1)

                         I           II          III       SEM

N balance (g/d)
  N intake *          9.32 (b)    8.99 (ab)    8.73 (a)   0.085
  NF *                2.64 (a)    2.76 (ab)    3.00 (b)   0.064
  NU                  3.09        2.88         3.10       0.097
  NB *                3.59 (b)    3.35 (b)     2.62 (a)   0.093
Growth performance
  Initial Wt (kg)     7.00        7.40         7.90       0.139
  Final Wt           11.80       11.78        11.76       0.146
  Wt change *         4.80 (a)    4.38 (a)     3.76 (b)   0.100
  ADG *              53.33 (a)   50.44 (ab)   41.78 (b)   0.343

NF = Nitrogen excreted through faeces; NU = Nitrogen excreted through
urine; NB = Nitrogen balance.

(a,b,c) Values bearing different superscript in a row differ
significantly * p<0.05 ** p<0.01.

SEM = Standard error of the mean. (1) For detail see Table 4.
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Author:Das, A.; De, D.; Katole, S.
Publication:Asian - Australasian Journal of Animal Sciences
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Geographic Code:9INDI
Date:Jan 1, 2011
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