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Early-life exposures and early-onset uterine leiomyomata in black women m the sister study.

BACKGROUND: Uterine leiomyomata (fibroids) are hormonally responsive tumors, but little is known about risk factors. Early-life exposures may influence uterine development and subsequent response to hormones in adulthood. An earlier analysis of non-Hispanic white women who participated in the Sister Study found associations between several early-life factors and early-onset fibroids.

OBJECTIVE: We evaluated associations of early-life and childhood exposures with early-onset fibroids among black women and compared the results with those found among white women.

METHODS: We analyzed baseline data from 3,534 black women, 35-59 years of age, in the Sister Study (a nationwide cohort of women who had a sister diagnosed with breast cancer) who self-reported information on early-life and childhood exposures. Early-onset fibroids were assessed based on self-report of a physician diagnosis of fibroids by the age of 30 years (n = 561). We estimated risk ratios (RR) and 95% confidence intervals (CI) from log-binomial regression models.

RESULTS: Factors most strongly associated with early-onset fibroids were in utero diethylstilbestrol (DES; RR = 2.02; 95% CI: 1.28, 3.18), maternal prepregnancy diabetes or gestational diabetes (RR = 1.54; 95% CI: 0.95, 2.49), and monozygotic multiple birth (RR = 1.94; 95% CI: 1.26, 2.99). We also found positive associations with having been taller or thinner than peers at the age of 10 years and with early-life factors that included being the firstborn child of a teenage mother, maternal hypertensive disorder, preterm birth, and having been fed soy formula.

CONCLUSIONS: With the exception of monozygotic multiple birth and maternal hypertensive disorder, early-life risk factors for early-onset fibroids for black women were similar to those found for white women. However, in contrast to whites, childhood height and weight, but not low socioeconomic status indicators, were associated with early-onset fibroids in blacks. The general consistency of early-life findings for black and white women supports a possible role of early-life factors in fibroid development.

KEY WORDS: diabetes meilitus, diethylstilbestrol, early-life, leiomyoma, multiple birth offspring, pregnancy, pregnancy-induced hypertension, prenatal exposure delayed effects, socioeconomic factors, soy formula. Environ Health Perspect 120:406-412 (2012). eh p. 1103620 [Online 2 November 2011]

Uterine leiomyomata (fibroids) are highly prevalent benign tumors (Baird et al. 2003) that are the principal indication for hysterectomies in the United States (Farquhar and Sreiner 2002). Fibroid-related morbidity may inclndc pelvic pain, heavy bleeding, and reproductive problems (Stewart 2001). Black women have an increased risk of developing fibroids and greater severity of symptoms than do white women (Baird et al. 2003; Faerstcin et al. 2001; Kjerulff et al. 1996; Marshall et al. 1997; Wise et al. 2005b). Although fibroids may be asymptomatic, prevalence estimates based on clinically evident disease have been as high as 50% for blacks and 25% for whites (Baird et al. 2003; Stewart 2001).

Although it is known that hormones influence fibroid development (Marsh and Bulun 2006), little is known about the risk factors that could explain these hormonal mechanisms. One hypothesis is that fibroid pathogenesis is influenced by early-life and childhood exposures that may affect the development of the uterus and later impact how a woman responds to hormonal challenges as an adult (Baird 2004). Proof of principle for this hypothesis is based on neonatal laboratory rodents exposed to experimental diethylstilbestrol (DES). In response to DES exposure, expression of estrogen-responsive genes was developmentally reprogrammed in the neonatal uterus, leading to fibroid development after maturity (Greathouse et al. 2008). Consistent with the laboratory animal findings, early data from non-Hispanic white participants in the Sister Study showed suggestive evidence of an elevated risk of early-onset uterine fibroids for women with in utero DES exposure (D'Aloisio et al. 2010). Other factors associated with early-onset fibroid development were having a mother diagnosed with diabetes before the index pregnancy (leading to participant's birth), early gestational age at birth, having been fed soy formula, and low childhood socioeconomic status (D'Aloisio et al. 2010). At the time of our previous analysis, enrollment of the Sister Study cohort was ongoing, and we did not have sufficient numbers to evaluate associations with early-life factors for black women. With enrollment now complete, we were able to evaluate whether early-life and childhood exposures were associated with self-reported early-onset fibroids using baseline data from black participants in the Sister Study and to compare these results with associations found among non-Hispanic whites from the complete cohort. Our study examined early-life factors related to the mother's index pregnancy and participant's birth and feeding during infancy, as well as childhood developmental and socioeconomic factors.

Materials and Methods

Study population. The Sister Study [National Institute of Environmental Health Sciences (NIEHS) 2011] is a prospective cohort study of 50,884 U.S. and Puerto Rican volunteer women 35-74 years of age who were not previously diagnosed with breast cancer but had a full or half sister who was diagnosed with breast cancer. Details of the study design have been described elsewhere (D'Aloisio et al. 2010). Study enrollment occurred between 2003 and 2009. The Sister Study was approved by the Institutional Review Board (IRB) of the NIEHS, National Institutes of Health, and the Copernicus Group IRB; all participants provided their informed consent. Computer-assisted telephone interviews assessed demographics, medical history, and possible risk factors for breast cancer and other conditions. Participants also completed a self-administered questionnaire on family history that included early-life events. Prepaid phone cards were given to participants to contact their mother or other relatives for assistance with completing family history questionnaires.

Our analysis included 3,534 black women who were 35-59 years of age when they cornpieced baseline enrollment activities in the Sister Study. Similar to our previous analysis, we excluded women [greater than or equal to] 60 years of age because of temporal trends in ultrasound use for fibroid diagnosis and because of the reduced likelihood that these women had a living mother to ask about early-life events (D'Aloisio et al. 2010). A total of 3,567 black women, 35-59 years of age, were available at baseline. We excluded 10 women with miss- ing data on fibroid status, 21 with missing age at fibroid diagnosis, and 2 with unlikely ages at fibroid diagnosis--for example, fibroids are rarely seen in girls < 12 years of age who have not begun menstruation. For comparison to results in black women, we updated our previously published results in non-Hispanic white women (n = 19,972), which were from an analysis done prior to completion of cohort enrollment (D'Aloisio et al. 2010). Data from 27,786 non-Hispanic white women (hereafter referred to as "whites") who were 35-59 years of age at baseline and who had complete data on fibroid diagnoses constituted the updated comparison group.

Fibroid assessment. During the baseline telephone interview, women reported whether a physician or health professional had ever diagnosed them as having uterine fibroids and their age at diagnosis. Because fibroids are highly prevalent and because their prevalence increases with age until menopause, many older women have undiagnosed fibroids. Therefore, we focused our study on early-onset fibroids to limit misclassification of women as noncases. For white women, we previously defined early-onset fibroids as a diagnosis at [less than or equal to] 35 years of age (D'AIoisio et al. 2010) based on ultrasound screening results that have shown that fibroid prevalence accelerates after 35 years of age (Baird et al. 2003; Laughlin et al. 2010). However, Laughlin et al. (2010) reported that the estimated age at onset may be about 10 years earlier for black women. Because our fibroid diagnoses are based on self-reported data, we used a conservative estimate to define early-onset fibroids in black women as a reported diagnosis at [less than or equal to] 30 years of age. We considered noncases to be all women who did not report earlv-onset fibroids, regardless of whether they reported a later fibroid diagnosis.

Exposure and covariate assessment. As previously described, most of the earlv-life exposures wrere ascertained in self-administered family history questionnaires that were completed at baseline (D'AIoisio et al. 2010). Early-life exposures included birth weight (pounds/ounces), categorical gestational age at birth, singleton or multiple birth, ever breastfed during infancy, ever fed soy formula, and maternal factors involving the index pregnancy (maternal age at birth, residence and work on a farm, smoking, DES use, prepregnancy diabetes, gestational diabetes, preeclampsia, and gestational hypertension). Gestational diabetes was defined as reporting gestational diabetes during the index pregnancy and reporting no history of diabetes before the index pregnancy. We defined mothers as having had gestational hypertension during the index pregnancy only if pregnancy-related high blood pressure was reported in the absence of preeclampsia. To allow for uncertainty, response options were "definitely," "probably," "probably not," and "definitely not" as well as "don't know" for most early-life questions. However, "don't know" was the only option available to address uncertainty for gestational age, birth weight, singleton or multiple birth, and maternal age at birth.

Questionnaires provided additional information on multiple births including number of babies delivered from the index pregnancy, sex of multiple birth sibling(s), and zygosity based on the participant's perception and test results when available. Using this information, we assigned twin births as monozygotic (reporting a twin sister who is genetically identical to the participant based on perception or testing), dizygotic (reporting a twin brother or a twin sister who is not genetically identical to the participant), or unknown (missing data to categorize type of twin birth). For the < 1% of black or white participants who reported having two or more multiple birth siblings, we combined them with twin birth categories according to the following criteria: monozygotic (all were genetically identical sisters), dizygotic (no sisters that are genetically identical to the participant), or unknown (missing data to categorize multiple births) or polyzygotic (having a genetically identical sister and a non-identical multiple birth sibling). Birth order was calculatcd using birthdates reported by participants for full siblings and half-siblings sharing the same mother. Participants reported sisters' birthdates during the telephone interview and brothers' birthdates in the family history questionnaire. Questionnaires also asked how long participants were breastfed or fed with soy formula, and whether soy formula consumption occurred during the first two months of life. Whether the participant's mother was alive at baseline was reported in the family history questionnaire.

Participants reported childhood exposures during the baseline telephone interview. Childhood socioeconomic factors included educational level of parents or guardians in the household when the participant was 13 years of age, family income level based on self-reported categories (poor, low, middle, or well off), and food insecurity (not having enough to eat at times during childhood). Developmental factors were age at menarche and height and weight relative to peers at age 10 years. Information on participant characteristics at baseline, including age, education, smoking status, alcohol intake, ages at live and still births, and menopausal status, were assessed during the telephone interview. Body mass index at baseline was calculated using home visit measurements of weight (pounds) and height (inches).

Statistical analyses. We estimated risk ratios (RR) with 95% confidence intervals (CI) using log-binomial regression for associations between each exposure and early-onset fibroid diagnosis. For infancy feeding and maternal exposures involving the index pregnancy (except for maternal age at birth), women were classified as exposed if they reported "definitely" or "probably" for a given factor and as unexposed if they reported "probably not" or "definitely not." We did not analyze data on how long participants were breastfed or fed with soy formula because a substantial proportion of participants did not know this information. For birth weight analyses, we created the following categories: < 2,500 g (low), 2,500-3,999 g, and [greater than or equal to] 4,000 g (high) based on clinical definitions of low and high birth weight and similarity of fibroid risk within categories. For maternal age and birth order, we created a combined index variable based on similarities in associations with fibroids and correlations between young maternal age at birth and being the firstborn using the following categories: < 20 years old and firstborn, [greater than or equal to] 20 years old and firstborn, <20 years old and not firstborn, and [greater than or equal to] 20 years old and not firstborn.

We considered variables as confounders if they either influenced reporting or were associated with the fibroid outcome and early-life and childhood exposures. Specifically, in all race-specific log-binomial regression models, we included participant's age and education because they may affect reporting of fibroids and exposures, and we included the maternal age at birth/birth-order index variable because it was associated with fibroids in our data and influences other early-life and childhood factors. We also adjusted for childhood family income in analyses for whites but not for blacks because childhood family income was only associated with the fibroid outcome for whites. Because of missing data on confounders, our adjusted analyses are based on 3,201 black women and 27,048 white women. For many of the observations excluded from the adjusted analyses, women did not complete family history questionnaires (n = 203 blacks; n = 361 whites), which resulted in missing data for maternal age at birth, birth order, and other early-life exposures. Despite evidence that early menarche is a risk factor for fibroids (Marshall et al. 1998; Wise et al. 2004), we did not adjust for age at menarche because it may be on the causal pathway between exposures of interest and fibroids. In secondary analyses, we further addressed whether other early-life factors may be acting as confounders by reevaluating associations after restricting analyses to smaller subsets of women without the potentially related factors. For example, we reevaluated the association with DES after excluding maternal conditions involving the index pregnancy (diabetes and hypertensive disorder). We conducted statistical analyses using SAS software (version 9.2; SAS Institute Inc., Cary, NC).

To assess whether our primary results for blacks were biased due to a high proportion of missing data for some early-life exposures, we also estimated associations with early-life exposures after performing multiple imputation analyses. These analyses assumed that missing data were not related to the actual values of missing variables but may have been associated with values of other study variables (i.e., we assumed that data were missing at random). We obtained ten imputation data sets from multiple imputation by chained equations using IVEware (version 0.1; University of Michigan, Ann Arbor, MI), which fits a series of regression models conditional on other variables and imputes missing data based on the values predicted for each participant in these models (Raghunathan et al. 2001, 2009). The multiple imputation regression models included all early-life factors (excluding soy formula consumption in the first two months of life because of multicollinearity with any soy formula consumption), childhood family income, participant's age, participant's education, mother's vital status at baseline, and early-onset fibroid status. Childhood family income and maternal vital status were not confounders in log-binomial regression models; however, they were included in multiple imputation regression models because they influenced whether early-life data were missing. Log-binomial regression analyses for associations with early-onset fibroids were repeated in the 10 imputation data sets as previously described, and regression estimates were combined using PROC MIANALYZE in SAS (version 9.2; SAS Institute Inc.). In secondaiy analyses after imputation of missing data, we also explored whether there was possible confounding among the early-life exposures by adjusting for additional early-life factors in log-binomial regression models. Multiple imputation of missing data allowed for simultaneous adjustment for several early-life factors in log-binomial regression models without reductions in sample size.


Ihe overall prevalence of self-reported fibroids among black women was 52%. Early-onset fibroids (diagnosed at [less than or equal to] 30 years of age) were reported by 16% of black women (Table 1). Black women who reported early-onset fibroids (cases) tended to be older at baseline (45-59 years of age, 82% vs. 79% of noncases) and were more likely to have received at least an associate's or technical degree (74% vs. 67%) than were women who did not report an early-onset diagnosis (Table 1). In addition, proportionally more cases (33%) than noncases (29%) reported no live or still births before 31 years of age, and cases were much more likely than were noncases to have reported that menarche began at [less than or equal to] 11 years of age (33% vs. 24%) and that they were surgically menopausal at baseline (56% vs. 33%) (Table 1). Furthermore, cases were three times as likely to have had a hysterectomy at [less than or equal to] 35 years of age than were noncases (26% vs. 8%), which suggests that many of the cases experienced severe fibroid-related morbidity.
Table 1. Baseline characteristics [n (%)]
by self-reported diagnosis of early-onset
uterine fibroids among black women, 35-59
years of age, in the Sister Study, 2003-2009 (n = 3,534). (a)

Characteristic Cases Noncases
Total 561 2,973 Body

Age (years) <25 77 514
 (13.8) (17.6)

35-44 102 634 25-29.9 173 905
 (18.2) (21.3) (30.9)

45-59 459 2,339 [greater 308 1,508

 than (51.5)

 (81.8) (78.7) or (55.2)

Highest Missing 3 46


High 32 310 Age
 (5.7) (10.4) at

school menarche

less (b) (years)

Some 116 659 <11 88 292
 (20.7) (22.2)
college, (15.7) (9.8)
Associate's 104 391 11 95 419
 (18.5) (13.2)
or (16.9) (14.1)

degree 12-13 278 1,574

Bachelor's 167 859 14 58 308

 (28.9) (10.4)
degree (29.8)

Graduate 142 753 [greater 42 376
 (25.3) (7.5)
 than (12.7)
degree (25.3) or


Missing 0 1 Missing 0 4

Maternal Parity
living at
status 30

Alive 276 1,498 0 184 865
 (52.9) (53.6)


Deceased 246 1,296 1 178 837
 (47.1) (46.4) (31.8)

Missingc 39 179 2 134 823
 (24.0) (27.8)
Smoking [greater 63 437
status than (11.3) (14.8)

Current 76(13.5) 352 Missing 2 11

Former 131 688 Menopausal
 (23.4) status

Never 354 1,933 Premenopausal 159 1,276
 (65.0) (28.3) (.43.0)

Alcohol Natural 87 727
intake (15.5) (24.5)
0 143 847 Surgical 315 965
 (28.6) (56.1) (32.5)

0.01-0.49 231 1,124 Missing 0 5

 (41.3) (37.9)

0.50- 104 511

2.00 (18.6) (17.3)

2.01- 55 314
6.99 (9.8) (10.6)
[greater 26 166
 (4.7) (5.6)



Missing 2 11

aCases are women with self-reported
diagnosis of fibroids at [less than
or equal to] 30 years of age. bLess
than high school includes 3 cases and
35 noncases. cData were missing for
203 women because they did not complete
family history questionnaires. rfTotal
number of live and still births before 31
years of age. eNatural menopause includes
women who had not had a menstrual period
in the past 12 months as long as they
were not pregnant, breastfeeding, or
using premenopausal medications that
cause amenorrhea. Surgical menopause
includes women whose menstrual periods
had ended before natural menopause because
they had had a hysterectomy, bilateral
oophorectomy, endometrial ablation, uterine
artery embolization, or chemotherapy.

Early-life factors. Eight early-life factors (DES, diabetes, hypertensive disorder, being the firstborn child of a teenage mother, low- birth weight, early gestational age at birth, multiple birth, and soy formula) were associated with at least a 20% relative increase in the risk of early-onset fibroid diagnosis among black women (Table 2). The strongest associations were with in utero DES (RR = 2.02; 95% CI: 1.28, 3.18) and maternal prepregnancy diabetes or gestational diabetes (RR = 1.54; 95% CI: 0.95, 2.49). The association with gestational diabetes (RR = 2.09; 95% CI: 1.00, 4.34) was stronger than the association with prepregnancy diabetes (Table 2). Excluding women who reported that their mothers had medical conditions during the index pregnancy (hypertensive disorder and prepregnancy diabetes or gestational diabetes) did not affect the association with in utero DES (RR = 1.95; 95% CI: 1.15, 3.30) based on 2,240 observations. In our previously published study of whites (D'Aloisio et al. 2010), we found that the association with DES was driven by women who reported probable rather than definite exposure. However, we estimated associations separately for this analysis of black women who reported definite and probable exposure to in utero DES, and we found that RRs were comparable (definite, RR = 1.87; 95% CI: 1.00, 3.50; probable, RR = 2.22; 95% CI: 1.15, 4.26). The multiple birth association appeared to be specific to monozygotic multiple births (RR = 1.94; 95% CI: 1.26, 2.99) (Table 2). After restricting analyses to singleton births and excluding women born at least 1 month early, we observed little change in the association between low birth weight and early-onset fibroids (RR = 1.27; 95% CI: 0.94, 1.71 based on I >833 observations). Although maternal farm residence during the index pregnancy was associated with early-onset fibroids, we found this association only among women who reported that their mothers had not worked on a farm during the index pregnancy.
Table 2. RRs for early-onset uterine
fibroids3 in association with early-life
exposures among black women, 35-59 years
of age, in the Sister Study, 2003-2009.

 Exposure Total [n = Cases(n) Adjusted RR (95%
 3,201 p CI)

Maternal pregnancy factors


Work and residence 418 59 0.88(0.68,1 13)

Work only 83 13 0.99

Residence only 85 22 1.48(1.03,

None 2,429 384 1.00

Missing 186 27

Smoking status^

Definitely/probably 738 130 1.18(0.98,1.42)

Definitely not/probably 2,161 322 1.00

Missing 302 53

DES use*7

Definitely/probably 42 13 2.02(1.28,3.18)

Definitely not/probably 2,352 368 1.00

Missing 807 124


Any 54 13 1.54 (0.95,

Prepregnancy 36 8 1.38

Gestational 16 5 2.09(1.00,

Unknown 2 0

None 2,598 407 1.00

Missing 549 85

Hypertensive disorder^

Any 176 36 1.38(1.01,1.87)

Preeclampsia 62 15 1.56

Gestational hypertension 82 17 1.41

Unknown 32 4

None 2,046 310 1.00

Missing 979 159

Exposure Total = Cases Adjusted RR (95%
 3.201)6 [n) CI)

Birth and infancy factors

Maternal age at birth/birth

< 20 years/1 st 183 41 1.43(1.08,1.90)

>20 years/1 st 261 34 0.83(0.60,1.15)

< 20 years/2nd or later 144 21 0.97(0.65,1.45)

> 20 years/ 2nd or later 2,613 409 1.00

Birth weight (g)

<2,500 335 67 1.31

2,500-3,999 1,530 239 1.00

> 4,000 131 17 0.84

Missing 1,205 182

Gestational age at birth

Born > 1 months early 83 19 1.43(0.94,

Born 2-4 weeks early 61 12 1.35

Not born > 2 weeks early 951 155 1.00

Missing 2,106 319

Multiple birth

Any 133 29 1.42(1.02,1.97)

Monozygotic 43 14 1.94(1.26,

Dizygotic 69 10 0.98

Unknown or polyzygotic 21 5

None 3,057 472 1.00

Missing 11 4

Fed breast milkc

Definitely/probably 1,438 250 1.18(0.99,1.40)

Definitely not/probably 1,385 200 1.00

Missing 378 55

Fed soy formula^

Definitely/probably 96 19 1.26

Definitely not/probably 2,486 411 1.00

Missing 619 75

Fed soy formula (age < 2

Yes 39 9 1.48

No 2,553 422 1.00

Missing 609 74

(a) 'Early-onset uterine fibroid status was
based on self-reported diagnosis at [less
than or equal to] 30 years of age (adjusted:
n = 505). (b) Each adjusted model included
the following core variables: participant's
age and education and maternal age/birth-order
index. Women with missing data for core
variables were excluded from all models.

Comparisons between blacks and whites. Early-life and childhood exposures that were associated with at least a 20% relative increase or decrease in early-onset fibroid risk for either black or white women are shown in Figure 1; we included results that we updated from the previous study of white women (D'Aloisio et al. 2010) [see Supplemental Material, Tables 1 and 2( for the estimated associations for all study exposures among the full cohort of eligible white women in the Sister Study]. The proportion of women who reported early-onset fibroids was 8% for white women (diagnosed at [less than or equal to] 35 years of age) versus 16% for black women (diagnosed at [less than or equal to] 30 years of age). Earlv-lifc factors associated with early-onset fibroids among blacks were similar to those among white women with the exception of monozygotic multiple birth status, which was only associated with early-onset fibroids among black women. In addition, associations with maternal hypertensive disorder and low birth weight were stronger among black women than among white women. For maternal diabetes, gestational diabetes was more strongly associated with early-onset fibroids than was prepregnancy diabetes among blacks, whereas the association was stronger for prepregnancy diabetes than gestational diabetes among whites (RR = 1.83; 95% CI: 1.10, 3.03 and RR = 1.22; 95% CI: 0.72, 2.05, respectively).

There were larger differences in associations with childhood exposures between black and white women. Taller height and thinner weight at age 10 years were associated with early-onset fibroids only for black women while associations with factors indicating low childhood socioeconomic status were generally evident only for whites.

Imputation analyses. Data on each of the early-life factors except maternal age at birth/birth-order index and multiple birth status were missing for at least 10% of black women. We performed multiple imputations of missing data for nearly all early-life factors and repeated our analyses of associations with fibroids [see Supplemental Material, Table 3 (]. Results from multiple imputation analyses were generally consistent with those shown in Table 2. After imputation of missing data, we adjusted our analyses for additional early-life factors in log-binomial regression models to evaluate possible confounding. Associations with maternal diabetes and hypertensive disorder were unaffected by further adjustment for multiple birth status (results not shown). The in utero DES association was only slightly attenuated (RR = 1.68; 95% CI: 0.98, 2.87) after repeating analyses with adjustment for maternal conditions (diabetes and hypertensive disorder) and multiple birth status, which are factors associated with higher risk pregnancy and hence a potentially increased likelihood that mothers were prescribed DES. However, the association was weakened for low birth weight (RR = 1.12; 95% CI: 0.85, 1.47) but was similar for having been born at least 1 month early (RR = 1.35; 95% CI: 0.87, 2.08) after inclusion of both early-life factors in the same model and adjustment for in utero DES, maternal diabetes, maternal hypertensive disorder, and multiple birth status.
Table 3. RRs for early-onset uterine fibroids" in association with
childhood socio-economic and developmental factors among blacks,
35-59 years of age, in the Sister Study, 2003-2009.

Exposure Total Cases Adjusted RR (95%
 (n- (n) CI)
 ) (b)

Maximum household
education (participant age
13 years)

< High school 1,050 160 0.94(0.79,1.12)

[greater than or equal 2,089 335 1.00
to]High school

Missing 62 10

Family income

Poor 557 90 1.02(0.82,1.28)

Low 1,134 IHl 1.01(0.84,1.21)

Middle/well off 1,499 232 1.00

Missing 11 2

Not enough to eat

,'CS 572 101 1.15(0.94,1.40)

No 2,623 404 1.00

Missing 6 0

Height relative to peers
(age 10 years)

Taller 832 154 1.24(1.03,1.50)

Same 1,419 212 1.00

Shorter 942 139 0.99(0.82,1.21)

Missing 8 0

Weight relative to peers
(age 10 years)

Heavier 538 83 1.09(

Same 1,343 190 1.00

Thinner 1,304 231 1.23(1.03,1.46)

Missing 16 1

(b) Early-onset uterine fibroid status was based on self-reported
diagnosis at < 30 years of age (adjusted: n = 505). *Each adjusted
model included the following core variables: participant's age and
education and maternal age/birth-order index. Women with missing
data for core variables were excluded from all models.


We found that black women had an increased risk of early onset fibroids in association with several early-life factors. Many of these associations were also found among white women with the exception of maternal hypertensive disorder and monozygotic multiple birth status. For black women, we also found that early-onset fibroids were associated with taller height and thinner weight at 10 years of age compared with peers, but no associations were observed with indicators of low socioeconomic status during childhood.

Misclassification of self-reported fibroids is a potential bias because fibroid risk increases with age, and many women who develop fibroids may never be diagnosed by a physician (Laughlin et al. 2010). Iherefore, we only considered early-onset diagnoses to improve sensitivity by reducing misclassificarion in the noncase group. For whites, we had defined early-onset cases as those occurring at [less than or equal to] 35 years of age (D Aloisio et al. 2010); however, fibroids develop at an earlier age in blacks (Laughlin et al. 2010). Iherefore, to reduce misclassihcation in the noncase group to rhe same degree in black women, we defined early-onset cases as those occurring at [less than or equal to] 30 years of age. We found that the estimated proportion of black women with early-onset fibroids in our study (16%) was consistent with the proportion of middleaged black women (19%) with self-reported fibroids by age 30 years in the NIEHS Uterine Fibroid Study (Baird Dl), unpublished data). In addition, cases were much more likely than noncases to report surgical menopause and early hysterectomies, which arc expected consequences for women with fibroids, and this supports that the specificity of self-reported early-onset fibroids is likely to be very high in our study.

Given that the majority of exposures occurred in utero or during infancy, exposure misclassification is another potential source of bias. However, participants received prepaid phone cards to encourage them to contact their mothers or other relatives to ask about early-life events, and we excluded women > 59 years of age who were less likely to have living relatives to consult. In addition, response categories for most of the early-life exposures allowed for additional uncertainty' in reporting with definite and probable categories. We repeated the analyses by evaluating associations separately for the reporting of definite and probable exposure, and we did not find that the reported associations with DES or any of the other early-life factors were unduly influenced by reporting probable exposure (results not shown). It is unlikely that differential exposure misclassification as a result of recall bias would have occurred in our study because none of the exposures are established risk factors for fibroids, instead, any exposure misclassification in our study would likely be nondifferential, which would generally result in RR estimates biased toward the null.

Strengths of this study include a focus on black women who have been shown to have greater fibroid incidence and morbidity than do white women (Baird et al. 2003; Faerstein et al. 2001; Kjerulff et al. 1996; Marshall et al. 1997; Wise et al. 2005b). Despite the small numbers of exposed cases for many of the early-life exposures, we were able to replicate many of the early-life findings from our previous analysis of white women with the exception of unique associations with monozygotic multiple birth status and maternal hypertensive disoider in black women. However, we found larger differences between blacks and whites in associations with childhood factors (D'Aloisio et al. 2010). Although > 10% of black women had missing data for most of the early-life exposures, results of multiple imputation analyses suggest that missing data did not bias reported associations.

In utero. DES was one of the early-life exposures chat was most strongly associated with early-onset fibroids among black women. Furthermore, the association between in utero DES and early-onset fibroids remained after excluding women who reported that mothers had medical conditions (diabetes and hypertensive disorder) during the index pregnancy, which may have increased the likelihood of treatment with DES. there fore, these conditions were unlikely to have confounded the association with DES. Evidence from animal studies shows that DES exposure during early development can increase fibroid incidence in adulthood (Cook et al. 2007). Previous epidemiological studies of in utero DES and fibroids have reported inconsistent findings, possibly due to differences in study design and assessment of DES exposure and fibroids (Baird and Newbold 2005; Missmer et al. 2004; Wise et al. (2005a). In particular, Wise et al. (2005a) found no association between medically documented DES exposure and surgical fibroid cases in a prospective study of women from two cohorts. Using data from a prospective study of endometriosis in Nurses' Health Study participants with intact uteri, Missmer et al. (.2004) noted that there was no association between self-reported DES exposure and uterine fibroids. However. Baird and Newbold (2005) indicated a positive association with self-reported DES exposure based on small numbers of exposed cases among black and white women from the N'lEHS Uterine Fibroid Study, which used ultrasound screening to identify fibroids and included black women in about 60% of the study sample. In addition, our finding for black women was similar to that for white women in the Sister Study. However, it is possible that women in the Sister Study who reported DES exposure may have received more gynecologic screenings, which could have led to a greater likelihood of detecting early-onset fibroids. Further studies of DES are needed in different study populations to support involvement in fibroid pathogenesis.

Another in utero factor associated with fibroids in black women was maternal diabetes--this association was slightly stronger for gestational diabetes than for prepregnancy diabetes. However, associations for gestational and prepregnancy diabetes were more similar in analyses after multiple imputation of missing data. Among whites, we also found a similar association with maternal diabetes, although the association was strongest for prepregnancy diabetes. We did not have information on the status of post-pregnancy diabetes in the participants' mothers, and distinguishing gestational diabetes from young onset of type 2 diabetes is difficult because limited access to care may have influenced whether mothers received a diagnosis of diabetes before pregnancy, if black women had poorer access to care than did white women, this may explain the differences in results for the two groups. As previously discussed, biological support exists for in utero exposure to diabetes influencing later risk of fibroids via developmental changes that may affect epigenetic patterns in areas pertaining to regulation of relevant genes for fibroid pathogenesis (D'Aloisio et al. 2010). There were few cases of maternal diabetes among black women in the cohort possibly reflecting limitations in available medical care for participants' mothers. In the United States, blacks have greater rates of type 2 and gestational diabetes than do whites (Egede and Dagogo-Jack 2005; Savitz et al. 2008); therefore, blacks are more likely to have in utero exposure to hyperinsulinemia and hyperglycemia that result in adverse developmental consequences. Thus, in utero exposure to maternal diabetes may contribute to the elevated fibroid burden among U.S. black women.

We previously reported an association between soy formula and early-onset fibroids among whites (D'Aloisio et al. 2010). Associations with having been fed soy formula (ever fed soy formula and fed soy formula within the first 2 months of life) were of similar magnitude for blacks and for whires, although the estimates for blacks were more imprecise. As previously discussed, soy formula might influence later risk of fibroids because of the high concentration of estrogenic isofla-vones (D'Aloisio et al. 2010). This hypothesis is further supported by effects on the reproductive system observed in neonatal laboratory rodents administered genistein, the hormonally activated form of the isoflavone predominantly found in soy formula (Jefferson and Williams 2011). There have been few studies of soy formula in relation to reproductive consequences in humans. However, one study reported that having been fed soy formula was associated with longer menstrual bleeding and greater pain with menstruation (Strom et al. 2001), both of which are also symptoms of fibroids.

Our study of black women is the first to report a strong association between early-onset fibroid risk and multiple birth, specifically for having been part of a monozygotic multiple birth. A possible causal mechanism is unclear but may be due to a greater likelihood of fetal growth restriction and resulting metabolic consequences in multiple births versus singleton births. More than half of monozygotic multiple births are monochorionic, which may lead to a greater risk of adverse outcomes than for dizygotic multiple births (Vaag and Poulsen 2007). We also found an association with maternal hypertensive disorder that persisted after accounting for multiple birth status. Preeclampsia and gestational hypertension are more likely to occur in the U.S. black population than in whites (Tanaka et al. 2007). Maternal hypertensive disorder may affect placental development leading to fetal malnourishment, which has been associated with increased risk of metabolic diseases in adulthood (Anderson 2007). Among black women, there were associations with early gestational age at birth and with low birth weight, which remained after excluding multiple births and those born at least one month before their due date. However, approximately 40% of women were missing birth weight data, and > 60% were missing data on gestational age at birth; adjusting for other early-life confounders in multiple imputation analyses resulted in attenuation of the association with low birth weight, although an association remained with having been born at least 1 month early. An association with having been born preterm was also found among white women, but the RR estimate for whites was more precise than for blacks. We previously discussed the possible biological plausibility for an association with having been born preterm based on not receiving enough estrogen for adequate uterine development (D'Aloisio et al. 2010).

With the possible exception of having a teenage mother, we did not find associations between fibroids and indicators of low socioeconomic status during childhood among black women, as we did for whites. Low childhood socioeconomic status indicators such as parent education and self-reported family income level may have a different societal context for blacks and whites, which may differentially influence adult health outcomes (Shavers 2007). Nevertheless, it is also possible that other sources of stress such as perceived racism (Wise et al. 2007) may have more relevance in fibroid pathogenesis for black women.

We also found associations with self-reported indicators of childhood height and weight among blacks but not among whites. Specifically, we found early-onset fibroid associations with having been taller than peers at age 10 years and having been thinner than peers at age 10 years. These associations did not change after including both these variables in the same model (results not shown), which suggests that these are independent associations. Even though early menarche is a risk factor for fibroids"(Marshall et al. 1998; Wise et al. 2004), it may be on the causal pathway because of its relations with greater childhood body fat (Ahmed et al. 2009) and taller childhood height (Freedman et al. 2002). Nevertheless, we verified that further adjustment for age at menarche did not affect our findings with childhood weight and height (results not shown). We also further excluded women who reported low birth weight (because size at birth may influence later childhood weight) and found that the association with thinner childhood weight did not change (result not shown). However, we acknowledge that self-reporting by adults of childhood height and weight relative to peers has limitations in that they are not absolute measures of height or weight and reflect participants' perceptions of their size relative to peers as well as their peer group. Terry et al. (2007) reported no association with childhood body fatness using data from the Nurses' Health Study, which is consistent with our findings in whites.


Our findings in blacks replicate many of our previously published associations with early-life factors and fibroids in whites (D'Aloisio et al. 2010). Biologically plausible mechanisms exist lor these factors to promote fibroid pathogenesis in adulthood. Despite largely consistent findings between blacks and whites, most of these early-life factors have not been investigated in other study populations, so further replication is needed to support their possible involvement in fibroid pathogenesis. Although many exposures such as diabetes and pregnancy complications were relatively rare in our cohort, their prevalence in the general population is likely growing given the obesity epidemic in the United States.

Address correspondence to A, D'Aioisio, Epidemiology Branch, National Institute of Environmental Health Sciences, P.O. Box 12233, Mail Drop A3-05, 111 T.W. Alexander Dr., Research Triangle Park NC 27709 USA. Telephone: (919) 541-3225. Fax: (919) 541-2511. E-mail:

Supplemental Material is available online (http:// 1103620).

This research was supported by the imramural Research Program of the National Institutes of I lealth, National Institute of Environmental Health Sciences (Z01 ES044005).

The authors declare they have no actual or potential competing financial interests. Received 1 March 2011; accepted 2 November 2011.

Figure 1. Comparisons in associations [RR (95% CI)] of selected early-life and childhood exposures with early-onset fibroids between black and white women, 35-59 years of age, in the Sister Study, 2003-2009.


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Aimee A. D'Aloisio, Donna D. Baird, Lisa A. DeRoo, and Dale P. Sandler

(1)Epidemiology Branch, National Institute of Environmental Health Sciences, National Institutes of Health, Department of Health and Human Services, Research Triangle Park, North Carolina, USA
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Title Annotation:Research
Author:D'Aloisio, Aimee A.; Baird, Donna D.; DeRoo, Lisa A.; Sandler, Dale P.
Publication:Environmental Health Perspectives
Article Type:Report
Geographic Code:1USA
Date:Mar 1, 2012
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