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Byline: M. Raheel, N. Javed, S. A. Khan and S. Ahmed


Entomopathogenic nematodes as alternatives to chemical pesticides for management of insect pests of various crop is an environmentally safe approach. Compatibility of Steinernema feltiae, S. asiaticum, Heterorhabditis bacteriophora and H. indica with seven chemical and biopesticides (imidacloprid (0.60 ml/L), spinosad (0.45 g/L), azadirachtin (1.5 ml/L), abamectin (1.25 ml/L), emamectin (0.20 ml/L), lambda-cyhalothrin (0.15 ml/L) and radiant (1.5 g/L) against Galleria mellonella was evaluated in lab. H. bacteriophora survived best as compared to all other entomopathogenic nematodes (EPN) species in all tested chemicals. S. feltiae survival was higher in imidacloprid followed by lambda-cyhalothrin, while abamectin proved to be lethal for S. feltiae. Azadirachtin and lambda-cyhalothrin proved to be compatible with H. bacteriophora as its survival was higher as compared to the rest of chemicals while radiant proved to be less friendly for its survival.

Emamectin followed by abamectin were more compatible with S. asiaticum whereas radiant was least compatible. Exposure of H. indica to spinosad and emamectin resulted in greater survival of nematodes while survival was lower when applied with lambda-cyhalothrin and radiant. Survival of S. feltiae and H. bacteriophora was higher when compared to H. indica and S. asiaticum. The infectivity of S. feltiae was the maximum when used with imidacloprid and lambda-cyhalothrin. H. bacteriophora proved to be more compatible with imidacloprid, azadirachtin, emamactin and lambda-cyhalothrin. S. asiaticum and H. indica were compatible with emamectin. Among all tested chemicals, EPN species were sensitive to abamectin. These results showed that EPN species. with better compatibility with evaluated pesticides may be included in Integrated Pest Management (IPM) Programs.

Key words: Compatibility, entomopathogenic nematodes, Steinernema feltiae, S. asiaticum, Heterorhabditis bacteriophora, H. indica, Galleria mellonella, chemicals, biopesticides.


Entomopathogenic nematodes (EPNs) are soil inhabiting insect pathogens being successfully used in agricultural systems not only against insects but also against Root Knot Nematodes (Kaya and Gaugler, 1993; Javed et al., 2012; Aatif et al., 2015).They have beneficial relationship with bacteria as Photorhabdus spp. present inside Heterorhabditis and Xenorhabdus spp. live in Steinernema (Raheel et al., 2015, 2017). Due to their ability to survive without food, EPNs can be used for insect control purposes. Entomopathogenic nematodes have been proved the most effective against more than 300 species of insects (Shahina and Tabassum, 2010). Like other parasitoids or predators, EPNs have chemo-receptors and are motile in nature. They can be cultured both in vitro and in vivo conditions, and are extremely virulent to their hosts (Ehlers, 2001).

There were also no difficulties in applying EPNs, as they are easily sprayed using standard equipment's including pressurized, mist, fan, electrostatic and aerial sprayers, and can be combined with almost all chemical control compounds (Georgis and Kaya, 1998; Ruhela, 2008; Radova, 2010, 2011). These were used against various insect pests both on foliage, and in the soil under cryptic habitats. As the best conditions for action and survival of EPNs were offered by the soil which is their natural habitat (Gaugler, 1988). Hence, opportunity to use EPNs is promising because a portion of the life circle of many insects (above 90%) is spent in the soil. Therefore, it is necessary to assess their compatibility with chemical and bio pesticides for insect management purposes.

The pesticides, bioproducts and plant extracts (imidacloprid (0.60 ml/L), spinosad (0.45 g/L), azadirachtin (1.5 ml/L), abamectin (1.25 ml/L), emamectin (0.20 ml/L), lambda-cyhalothrin (0.15 ml/L) and radiant (1.5 g/L) were evaluated against the survival of four EPN species (H. bacteriophora, H. indica, S. feltiae and S. asiaticum).


Nematodes: Heterorhabditis bacteriophora, H. indica, Steinernema feltiae and S. asiaticum were used for experimentation.

G. mellonella: The larvae of G. mellonella were reared on cereal diet in laboratory (Wiesner, 1993).

EPNs culturing: Entomopathogenic nematodes were cultured on G. mellonella larvae. The freshly hatched EPNs were placed in shallow plastic containers having covers with suspension being no more than 1 cm in depth to confirm enough availability of oxygen at 10degC. The in vivo produced nematodes could be kept for 3 months. All stocked nematode cultures were again cultured after every 4 months. For current study fresh culture of EPNs (less than 2 weeks old) was used.

For the analysis of survival of these EPN species stock solutions of these chemicals were made in water at double strength of the necessitated dose. About 1500 IJs of all species in 0.5 mL of water were mixed with 0.5 ml of each stock solution in each well of the 24-well plates. These well plates were then kept at room temperature (20-25degC). There were five replications of each treatment. In control treatment water was used. The products were examined in groups of four to eight chemicals with a control treatment included each time. This was carried out for comfort of handling.

Under a stereomicroscope the viability of IJs incubated in various chemicals was evaluated after 24h from each well of the 5 replicates. One hundred IJs were counted for each treatment and the control. Infective Juveniles which did not show any movement after prodding were considered dead (Elizabeth et al., 2003). The experimental units were arranged under CRD design and the data was analyzed statistically. The viable nematodes after 24 h exposure to pesticides/biopesticides (imidacloprid, spinosad, azadirachtin, abamectin, emamectin, lambda-cyhalothrin and radiant) were washed by using 400 mesh sized sieve and pouring distilled water on the IJs.

The infectivity of nematodes against larvae, after treatment with all the pesticides/biopesticides, was examined by using the 1:1 sand-well bioassay (Grewal et al., 1999) in 24-well plates. Sieved sand was washed and then allowed to dry. Sub samples from each treatment were removed from the wells, placed in 10 cm petri dishes, and diluted with water. Five IJs were picked with a micropipette in 90 mL of water under a stereomicroscope and were placed in each well containing 1.5 g of sterilized dry sand. One larva of G. mellonella was placed in each well and the plates were incubated at 22degC and 60% RH. The mortality of G. mellonella larvae was evaluated after 6 days of incubation while the control treatment had no pesticides/biopesticides. There were three replications of each treatment and experimental units were arranged in completely randomized design. The data was analyzed statistically.


ANOVA for survival of EPNs at different soil moisture levels showed that effect of treatments and species was significant (p>0.05). Interaction effect of treatments and species was also significant. Maximum mortality was observed in S. feltiae (42%) when used with abamectin, followed by S. asiaticum (29.4%) with radiant. The mortality was recorded minimum in case of S. feltiae (0.2%) when used with imidacloprid. Hence, the survival of S. feltiae was significantly higher with imidacloprid followed by lambda-cyhalothrin and spinosad, while abamectin proved to be lethal for its survival (45% mortality). Effect of azadirachtin and lambda-cyhalothrin on survival of H. bacteriophora was significantly more friendly than rest of biopesticides used (as exhibited by least mortality of IJs) and radiant proved to be less friendly for its survival.

Emamectin followed by abamectin were significantly more compatible with S. asiaticum whereas it was sensitive to radiant. Exposure of H. indica to spinosad and emamectin resulted in significantly higher survival while its survival was significantly lower against lambda-cyhalothrin and radiant. (Fig 1). H. bacteriophora showed best results against all biopesticides compared to other species.

ANOVA for infectivity of EPNs with combination of different pesticides/biopesticides showed that effect of treatments and species was significant (p>0.05). Interaction effect of treatments and species was also significant. The pathogenicity/infectivity of S. feltiae and H. bacteriophora was significantly higher as compared to H. indica and S. asiaticum. The infectivity of S. feltiae was significantly higher when used with imidacloprid, spinosad, emamectin and lambda-cyhalothrin. Whereas, H. bacteriophora proved to be more compatible with imidacloprid, azadirachtin, emamectin and lambda-cyhalothrin. Steinernema asiaticum and H. indica were more compatible with emamectin. Out of 7 chemicals tested abamectin was most destructive as S. feltiae nematodes were not able to infect the larvae of G. mellonella it was followed by radiant in case of S. feltiae and S. asiaticum (Table 1).

Overall H. bacteriophora showed best infectivity against larvae of G. mellonella when used with all biopesticides compared to other species.

Table 1: Effect of EPNs treated with various chemical and Bio-pesticides on Greater Wax Moth Larvae



S. feltiae###56.67B###55.33B###43.33EF###00.00I###55.33B###56.67B###36.67G###67.33A

H. bacteriophora###55.33B###52.67BC###56.67B###44.67DEF###54.00B###56.67B###46.00DE###67.33A

S. asiaticum###46.00 DE###43.33EF###43.33EF###44.67DEF###55.33B###46.00DE###31.33H###68.67A

H. indica###48.67CD###47.33DE###48.67CD###48.67CD###52.67BC###46.00DE###40.67FG###68.67A


Entomopathogenic Nematodes and chemical pesticides deal different but compatible tactics for management of insect population. EPNs may find out their host in unreachable parts where chemical pesticides may not perform. Therefore, efforts should be made to use EPNs in Integrated Pest Management (IPM). Though, compatibility of EPNs with chemicals was studied by invitro bioassays with direct exposure of EPNs to insecticides, still it is required to evaluate the harmful effects of these chemicals on EPNs, as the formulations of various insecticides may differ in toxicity to different nematode species due to the use of different surfactants. Furthermore, EPNs species of different origins may also vary in sensitivity to various formulations of same insecticides (Krishnayyaand and Grewal, 2002).

In recent past studies have found that many species of EPNs were relatively not sensitive to many pesticides (Alumai and Grewal, 2004; Radova, 2010, 2011; Bortoluzzi et al., 2013; Sabino et al., 2014; Ulu et al., 2016), although some carbamates and organophosphates proved to be lethal to IJs of S. carpocapsae. Moreover, few chemicals proved to be harmful to the virulence of nematodes (Radova, 2011; Bortoluzzi et al., 2013).

The recent work evaluated the important chemical and bio insecticides commonly applied in IPM system against different insects in Pakistan. The findings of the recent work and those of previous studies (Krishnayyaand and Grewal, 2002; De Nardo and Grewal, 2003; Radova, 2011; Laznik et al., 2012) in which EPNs compatibility with plant protection products was accessed, showed that compatibility is species specific. Our results showed that azadirachtin did not affect the survival of S. feltiae and H. bacteriophora however, it did affect the survival of H. indica and S. asiaticum. Whereas, abamectin showed a nematicidal effect on EPN species which may explain the relatively high mortality values (Mahfooz et al., 2008). Moreover, it was resulted that H. bacteriophora was the most tolerant species as compared to S. feltiae, S. asiaticum and H. indica. Similar finding was reported (Radova, 2011; Laznik and Trdan, 2013).

In recent study no significant difference was observed on the survival of IJs of H. indica against radiant and emamectin. These findings were in confirmation to other observations (Lalramliana and Yadav, 2008).

Therefore, it is concluded that only IJs of selected species of EPN exhibit compatibility with tested plant protective chemicals, some others may also show injurious effects to these EPNs. The EPNs with better compatibility may be considered as strong option for usage in IPM. The EPN species S. asiaticum has not been tested for its efficacy when used with various chemical pesticides and plant products.


Aatif, H.M., N. Javed, S.A. Khan, S. Ahmed and M. Raheel (2015). Virulence of entomopathogenic nematodes against Meloidogyne incognita for invasion, development and reproduction at different application times in Brinjal roots. Int. J. Agric. Biol. 17 (5): 995-1000.

Alumai, A. and P.S. Grewal (2004). Tankmix compatibility of the entomopathogenic nematodes, Heterorhabditis bacteriophora and Steinernema carpocapsae, with selected chemical pesticides used in turfgrass. Biocontr. Sci. Tech. 14: 725-730.

Bortoluzzi, L., L.F.A. Alves, V.S. Alves and N. Holz. (2013). Entomopathogenic nematodes and their interaction with chemical insecticide aiming at the control of Banana weevil borer, Cosmopolites sordidus Germar (Coleoptera: Curculionidae). Arq. Inst. Biol., Sao Paulo. 80(02): 183-192.

De Nardo, E.A.B. and P.S. Grewal (2003). Compatibility of Steinernema feltiae (Nematoda: Steinernematidae) with Pesticides and Plant Growth Regulators Used in Glasshouse Plant Production. Biocontr. Sci. Tech.13:441 - 448.

Ehlers, R.U. (2001). Mass production of entomopathogenic nematodes for plant protection. Appl. Microbol. Biotech. 56: 623-633.

Elizabeth, A., B. De-Nardo and P.S. Grewal (2003). Compatibility of Steinernema feltiae (Nematoda: Steinernematidae) with Pesticides and Plant Growth Regulators Used in Glasshouse Plant Production. Biocontr. Sci. Tech. 13(4): 441-448.

Gaugler, R. (1988). Ecological considerations in the biological control of soil-inhabiting insects with entomopathogenic nematodes. Agric. Ecosystems Environ.24: 351-360.

Georgis, R. and H.K. Kaya (1998). Formulation of entomopathogenic nernatodes. Formulation of microbial biopesticides: Beneficial microorganisms nematodes and seed treatments. H. D. Burges. Dordrecht Kiuwer Academic Publishers: 289-308.

Grewal, P.S., V. Converse and R. Georgis (1999). Influence of production and bioassay methods on infectivity of two ambush foragers (Nematoda: Steinernematidae). J. Invertebr. Pathol.73: 40-44.

Javed, N., S.A. Khan, I.U. Haq, M. Atiq and M. Kamran (2012). Effect of Steinernema glaseri and H. Indica on the plant vigour and root knot nematodes in tomato roots at different densities and time of application. Pakistan J. Zool. 44:1165-1170.

Kaya, H.K and R. Gaugler (1993). Entomopathogenic nematodes: a developing biological control technology: In: Evans, K., (Ed.). Agricultural Zoology Reviews Intercept Andover 6: 63-94.

Krishnayyaand, P.V and P.S. Grewal (2002). Effect of neem and selected fungicides on viability and virulence of the entomopathogenic nematodes Steinernema feltiae. Biocontr. Sci. Tech. 12: 259-266.

Lalramliana and A.K. Yadav (2008). Compatibility of chemical pesticides with locally isolated entomopathogenic nematodes (Steinernematidae and Heterorhabditidae) from Meghalaya, Northeast India. Current trend in parasitology 1: 261-267.

Laznik, Z., M. Vidrih and S. Trdan (2012). Effect of different fungicides on viability of entomopathogenic nematodes Steinernema feltiae (Filipjev), S. carpocapsae Weiser and Heterorhabditis downesi Stock, Griffin and Burnell (Nematoda: Rhabditida) under laboratory conditions. Chil. J. Agr. Res. 72:62-67.

Laznik, Z. and S. Trdan (2013). The influence of insecticides on viability of entomopathogenic nematodes (Rhabditida: Steinernematidae and Heterorhabtidae) under laboratory conditions. Pest. Manag. Sci. doi: 10.1002/ps.3614

Mahfooz A., M. Z. Masood, N. Yousaf, N. Akhtar, M.A. Zafar (2008). Prevalence and athelmintic efficacy of abamectin against gastrointestinal parasites in horses. Pakistan Vet. J.28:76-78.

Radova, S. (2011). Effect of selected pesticides on survival and virulence of two nematode species. Polish. J. Environ. Stud. 20(1): 181-185.

Radova, S. (2010). Effect of selected pesticides on the vitality and virulence of entomopathogenic nematode Steinernema feltiae (Nematoda: Steinernematidae). Plant Protect. Sci. 46(02): 83-88.

Ruhela, A. (2008). Nematode Control in Crops. Oxford Books Company; New Dehli (India). 52-55p.

Raheel, M., N. Javed, S.A. Khan, H.M. Aatif and S. Ahmed (2017). Effect of temperature on reproductive potential of indigenous and exotic species of Entomopathogenic nematodes inside Galleria mellonella L. larvae. Pakistan J. Zool. 49(01): 395-397.

Raheel, M., N. Javed, S.A. Khan, and S. Ahmed (2015). Impact of soil texture on the infectivity of different species of Entomopathogenic nematodes against Greater Wax moth (Galleria mellonella L). Pakistan J. Phytopathol. 27(02): 189-192.

Sabino, P.H.S, F.S. Sales, E.J. Guevara, A. Moino. Jr. and C.C. Filgueiras (2014). Compatibility of entomopathogenic nematodes (Nematoda; Rhabditida) with insecticides used in the tomato crop. Nematoda. 1:e03014.

Shahina, F. and K.A. Tabassum (2010). Virulence of Steinernema pakistanense against different insect species in laboratory condition. Pakistan J. Nematol. 28(2): 279-284.

Ulu, T.C., B. Sadic and I.A. Susurluk. 2016. Effect of different pesticides on virulence and mortality of some entomopathogenic nematodes. Research report. Deptt. of Plant Protect., Faculty of Agri., Uludag Univ., Nilufer, Bursa, Turkey.

Wiesner, A. (1993). The induction of the Immunabwehreines insect (Galleria mellonella, Lepidoptera) durch synthetische Materialien and arteigene Haemolymphfaktoren. Berlin.
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Publication:Journal of Animal and Plant Sciences
Geographic Code:9PAKI
Date:Jun 30, 2017

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