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Digestibility of pelleted rations containing diverse potato flour and urea/ Degradabilidade ruminal e digestibilidade in vitro de racoes peletizadas contendo farinha de batata diversa e ureia.

INTRODUCTION

The state of Minas Gerais is the leading producer of potatoes (Solanum tuberosum L.) in Brazil, both in terms of crop growth estimates and production areas (Instituto Brasileiro de Pesquisa Agropecuaria - IBGE, 2013). However, a large quantity of potatoes do not meet the market standards because of damage caused during harvesting and processing, and these losses increase during periods of high availability of potatoes in the market.

These discarded potatoes are a type of crop residue called "diverse potatoes", which consist of tubers that failed to reach commercial standards (REZENDE et al., 2007), and they can be used as ruminant feed. However, potatoes are rapidly perishable and their production period is of few months, which makes their use as animal feed discontinuous. Thus, methods to promote the conservation of potatoes and extend their shelf life are necessary. Silage of diverse potato bran (REZENDE et al., 2007) or of other waste generated during industrial processing (SUGIMOTO et al., 2007, 2008) is the most widely adopted conservation method.

Pelletization can be used as an alternative method to recover potato residues, and it can promote the elimination of contaminating microorganisms, increase shelf life, make transportation and storage easier, and allow the addition of other ingredients to the residue.

Diverse potato flour mixed with urea and pelletized, similar to Starea (extruded starch + urea product; SALVADOR et al., 2004), can promote a gradual release of ammonia in the rumen, possibly reducing the risk of urea poisoning and improving the synthesis of microbial proteins.

Thus, the aim of this study was to evaluate in situ rumen degradability and in vitro digestibility of dry matter (DM) of concentrated supplements formulated using pelleted diverse potato flour with varying levels of urea.

MATERIAL AND METHODS

Diverse potatoes were ground in a stationary forage chopper to obtain pieces of an average size of 2cm. Subsequently, the potato pieces were dried in the sun until they had a moisture level of 15%, and they were then ground. The flour obtained was mixed with other ingredients (Table 1) to facilitate pelletization and to improve nutritional adequacy. Urea was added to the pellets at increasing levels (0%, 4%, 8%, and 12% DM), and four treatments were established (Table 1). Ingredients were mixed and pelletized in a 3/8 matrix HP7.5 pelletizer.

Rumen degradability test was conducted using a 4x4 Latin square (LS) design. Four male sheep (average body weight, 46.0 [+ or -] 5.3kg) of an undefined breed were used. Each sheep was fitted with a rumen cannula. The animals' diet consisted of coarsely ground Tifton 85 grass hay (Cynodon spp.) comprising 90.7% DM, 9.5% crude protein, and 76.6% neutral detergent fiber (NDF), and it was provided daily at 7 AM and 5 PM. The amount provided was approximately 3% of their body weight and was adjusted to account for leftovers of around 20%. Water and mineral supplements were provided ad libitum.

The incubation bags were made of nonwoven fabric (100g/[m.sup.2]) and measured 9.0x 14.0cm (MONTEILS et al., 2002); dried in a forced ventilation oven at 65[degrees]C for 48h; weighed; and filled with 4g of the evaluated feed (ORSKOV, 1992), which had been previously ground into 2-mm pieces (NOCEK, 1988). Feeds were incubated as recommended by NOCEK (1988) for 0, 2, 4, 8, 12, 24, 36, and 48h, according to a method used in previous studies (ORSKOV, 1992; TEIXEIRA et al., 1999; MONTEILS et al., 2002).

A total of 384 bags (four sheep x four treatments x eight times x three replicates) were incubated and distributed so that each animal received all three repetitions of each of the four treatments and in each phase of LS. Because of the small volume of sheep rumen, each animal received the bags for varying periods of decreasing duration, i.e., 48-, 36-, and 24-h periods, and were simultaneously withdrawn for further incubation for other periods. After the bags were removed, they were placed in a container with ice in order to halt fermentation and were subsequently frozen so all bags were simultaneously washed at the end of each incubation. The bags at time zero were placed in the container with ice and immediately removed, thereby receiving the same treatment as the other bags.

For washing, bags were placed in a container with water until the residue of the rumen contents was completely removed. After drying in an oven at 65[degrees]C for 72h, bags were weighed, and the remaining residues were analyzed for DM content.

Ruminal degradation parameters of DM were estimated using the Marquardt algorithm iterative process, according to the procedure for non-linear models (PROC NLIN; SAS, 2004). Data from partial degradation of DM for each treatment were adjusted per sheep, according to the equation proposed by MEHREZ & ORSKOV (1977), and subsequently subjected to linear and quadratic regression analyses ([alpha]=0.05) using PROC REG (SAS, 2004). Effective degradability (ED) values of DM were calculated according to the method proposed by ORSKOV & MCDONALD (1979), adopting passage rates of 2% [h.sup.-1], 5% [h.sup.-1], and 8% [h.sup.-1] in the rumen (ARC, 1980).

To evaluate in vitro digestible dry matter (IVDDM), rumen fluid was obtained from a fistulated male sheep of an undefined breed. The animal, which was adapted to consume pelleted diverse potato flour with urea, was fed with Tifton 85 grass hay and water, mineral salts, and supplementary feed were provided ad libitum. Contents of the rumen were collected before the morning feed and were immediately filtered. An artificial saliva solution was added to the rumen fluid at a 4:1 ratio, and it was then incubated with 5g each of the tested feeds, according to the two-stage technique described by TILLEY & TERRY (1963). After incubation, the remaining content of each tube was oven-dried at 105[degrees]C for 12h, weight of the residue was determined, and the residue was then analyzed for DM content.

The effect of urea levels on IVDDM was evaluated using a completely randomized design with six replicates through analyses of linear and quadratic regression ([alpha]=0.05) with PROC REG (SAS, 2004). LSMEANS of the GLM procedure was adopted to generate mean and standard error values.

RESULTS AND DISCUSSION

The amount of urea in the pellets had no effect (P>0.05) on the soluble (a) and potentially degradable (b) fractions (Table 2). These were considerably different from the fractions obtained for Starea, containing corn or cassava, with values ranging from 30.5% to 36.5% for fraction "a" (TEIXEIRA et al., 1999), as well as the silage of potato with or without urea treatment (30.3% and 38%, respectively) (SUGIMOTO et al., 2007).

Rumen degradability of starch in different feeds varied from 18.4% to 87.3% for fraction "a". Soluble fraction of starch obtained by washing the bags at time zero could be overestimated because of the loss of particles from inside the bag, thereby influencing the rumen degradability values of feeds and jeopardizing the estimate of parameters "b" and "c" (ZEOULA et al., 1999). Thus, factors such grain size and the source of grain and feed may influence starch degradability (GIUBERTI et al., 2013).

A quadratic effect (P=0.03) was observed for degradation rate "c," which ranged from 4.75% [h.sup.-1] to 7.39% [h.sup.-1] (Table 2); the maximum value estimated was 7.41% [h.sup.-1], and it was obtained when 5.9% urea was included in the pellet. The values observed (Table 2) were close to 6.6% [h.sup.-1] obtained for Starea 45S, with ground corn being the source of starch (TEIXEIRA et al., 1999). The values 6.3% [h.sup.-1] and 6.5% [h.sup.-1] were obtained for the silage of potato pulp with or without treatment with 0.5% urea, respectively (SUGIMOTO et al., 2007); and 7.4% [h.sup.-1] and 6.4% [h.sup.-1] for the silage of potato pulp with soybean meal or corn gluten, respectively (SUGIMOTO et al., 2008).

Quadratic effects (P<0.02) of urea content in pellets on the ED of DM have been observed, considering passage rates of 2% [h.sup.-1], 5% [h.sup.-1], and 8% [h.sup.-1] in the rumen (Table 2). Maximum ED values calculated under these passage rates in the rumen were estimated when 6.3-7.3% urea was included in the pellets. ED of DM estimated for 8% urea in the potato flour pellet (55%), considering a passage rate of 5% [h.sup.-1] in the rumen, was similar to that recorded for Starea 45S with corn as a source of starch in whole, broken, or in ground form (56.8%, 54%, and 57.4%, respectively). This indicates that pelletization permits synchronization between starch and urea release, which promotes microbial growth in the rumen (TEIXEIRA et al., 1999).

IVDDM of concentrated supplements was influenced in a quadratic manner (P=0.02) by the urea content in the pellet (Table 3), and the maximum value estimated was 96.9% when 7.9% urea was included in the pellet. This value is higher than the IVDDM of sun-dried potato peel residue (85.4%), which may be due to the high NDF concentration in the potato peel residue (41%; TAWILA et al., 2008) in comparison with treatments with diverse potato flour with an average NDF content of 11%.

The high values observed for IVDDM may result in increased DM intake and passage rate through the rumen (MALLMANN et al., 2006). This is relevant since the passage rate interferes with the reduction of the average age of microbiota because of removal of mature organisms. Thus, the energy required to maintain microbiota is reduced, thereby increasing the efficiency of energy use in the system for microbial growth (SALVADOR et al., 2004).

To increase the level of ammonia derived from degradable proteins or non-protein nitrogen sources in the rumen, IVDDM can be increased without necessarily increasing microbial activity, primarily in diets containing highly soluble urea (CALDAS NETO et al., 2007) because rumen microbiota have nitrogen requirements that are beyond the availability of ammonia (FIRKINS, 2010).

Levels of urea added to the pellet that was estimated to reach the maximum value for fraction "c" was 5.9%. For ED, it ranged from 6.3% to 7.3% for a maximum IVDDM of 7.9%. When the nutritional quality of the pellets was evaluated on the basis of these parameters, levels of urea in the pellets ranged from 5.9% to 7.9% or, in practice, 6-8%. Synchronization of carbohydrate and urea digestion rates in the rumen theoretically increases efficiency of microbial protein synthesis, promoting an optimal rumen environment to maintain animal health and consequently increase DM intake and productivity (TEIXEIRA, 1999). Addition of various levels of urea to the pellets resulted in a more available nitrogen for microbial activity, thereby culminating in higher ruminal degradability and IVDDM. However, branched-chain fatty acids and peptides may become a limiting factor for microbial activity at inclusion values above the 6-8% range, which partially explains the quadratic behavior observed for the degradation rate (c), ED, and IVDDM. Therefore, it is advisable to add 6-8% urea to pelleted feed made from diverse potato flour.

CONCLUSION

Our results suggest that we need to pelletize diverse potato flour and add 6-8% urea to obtain a large increase in the in vitro digestibility and ED of DM.

BIOETHICS AND BIOSSECURITY COMMITTEE APPROVAL

The procedures used during the animal experiments comply with the resolutions of Conselho Nacional de Controle de Experimentacao Animal (CONCEA).

http://dx.doi.org/10.1590/0103-8478cr20141234

ACKNOWLEDGEMENTS

This work was supported by Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq) (Process: 472606/2008-5). We like to thank Associacao dos Bataticultores de Minas Gerais (ABASMIG) the supply of potatoes and Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior (CAPES) for the Scholarship granted to I. Martinele.

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Isabel Martinele (I) Paulo Cesar de Aguiar Paiva (I) Fernando Cesar Ferraz Lopes (III) Juliana de Cassia Pereira (II) Ludhiana Rosa Ferreira (II)

(I) Departamento de Zoologia, Universidade Federal de Juiz de Fora (UFJF), 36036-900, Juiz de Fora, MG, Brasil. E-mail: isabelmartinele@yahoo.com.br. Corresponding author.

(II) Departamento de Zootecnia, Universidade Federal de Lavras (UFLA), Lavras, MG, Brasil.

(III) Embrapa Gado de Leite, Juiz de Fora, MG, Brasil.

Received 08.20.14

Approved 04.07.15

Returned by the author 07.14.15

CR-2014-1234.R3
Table 1--Composition of pelleted rations containing
diverse potato flour and urea.

Composition                  Urea level in the pellet (%)

                              0      4      8      12

Ingredients (% dry matter)
Diverse potato flour          60     60     60     60
Corn milled                   20     20     20     20
Wheat Bran                    17     13     09     05
Molasses                      03     03     03     03
Urea + Ammonium sulfate       0      4      8      12
  (9:1)
                               Chemical composition
                                  (% dry matter)

Dry matter                   87.9   89.8   88.7   85.1
Mineral matter               8.9    8.1    8.1    8.6
Organic matter               79.0   81.7   80.6   76.4
Starch                       52.7   55.7   52.1   51.4
Crude protein                8.9    19.8   28.8   36.7
Neutral detergent fiber      12.3   11.2   10.7   9.5
Ether extract                0.8    1.3    0.6    0.9

Table 2--Ruminal in situ degradation of dry matter of
pelleted rations containing diverse potato flour and
urea.

Variable (1)        Urea level in the      SEM (2)
                         pellet (%)

                0      4      8      12

a (%)          7.1    2.1    12.1   4.3      2.2
b (%)          78.2   85.2   72.9   87.5     2.8
c (%/h)        5.1    6.8    7.3    4.7      0.9

PD (%)         85.4   87.3   85.0   91.9     2.1
ED2 (%)        61.9   67.0   68.9   65.7     1.2

ED5 (%)        45.6   50.3   55.0   46.8     1.1

ED8 (%)        37.0   40.5   46.6   36.8     1.1

Variable (1)   Regression equation and P value

a (%)          y = 6.43 (P = 0.91)
b (%)          y = 80.96 (P = 0.42)
c (%/h)        y = 5.05513 + 0.80159X - 0.06824[X.sup.2];
                 [r.sup.2] = 0.31; P = 0.03
PD (%)         y = 87.40 (P = 0.18)
ED2 (%)        y = 61.85325 + 1.89269X - 0.13008[X.sup.2];
                 [r.sup.2] = 0.44; P = 0.02
ED5 (%)        y = 45.01163 + 2.62416X - 0.20176[X.sup.2];
                 [r.sup.2] = 0.45; P = 0.01
ED8 (%)        y = 36.07738 + 2.65872X - 0.21012[X.sup.2];
                 [r.sup.2] = 0.40; P = 0.01

(1) where: a = soluble fraction; b = potentially
degradable insoluble fraction; c = rate of degradation
of fraction b; PD = potential degradability
(a + b); ED2, ED5, ED8 = effective degradability
considering passage rate in the rumen of 2; 5 and 8%
[h.sup.-1].

(2) Standard error of the mean.

Table 3--In vitro digestibility of dry matter (%) of
pelleted rations containing diverse potato flour and urea.

          Urea level in the pellet (%)    Regression equation
                                          and P value
            0       4       8      12

Mean      92.06   96.29   96.42   95.87   y = 92.23467 + 1.18533X
                                          - 0.07469[X.sup.2];

SEM (1)   0.88    0.88    0.88    0.88    [r.sup.2] = 0.36;
                                            P = 0.02

(1) Standard error of the mean.
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Title Annotation:produccion animal; texto en ingles
Author:Martinele, Isabel; Paiva, Paulo Cesar de Aguiar; Lopes, Fernando Cesar Ferraz; Pereira, Juliana de C
Publication:Ciencia Rural
Date:Nov 1, 2015
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