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Diagnostic value of neutrophil to lymphocyte ratio in differentiation of ruptured ovarian cysts and adnexal torsion/Notrofil lenfosit oraninin rupture over kisti ve adneksiyel torsiyon ayrimindaki tanisal degeri.

Introduction

Adnexal torsion (AT) can be defined as total or partial rotation of the adnexa around its own vascular axis. It results in venous and lymphatic blockage of ovarian tissue and causes congestion and hemorrhagic infarction leading to gangrene and hemorrhagic necrosis (1). AT cases are generally seen in the reproductive age group (2). Although AT has the highest incidence among women aged between 20 and 30 years, it can be experienced by women at any age. It is diagnosed in 2-7% women who undergo surgery for acute pelvic pain (3).

The diagnosis of AT is important for preserving fertility. Early diagnosis and detorsion of the adnexa may preserve ovarian function and fertility. Although conventional methods are used for the diagnosis of AT, only 46% of patients who are diagnosed as having AT have real torsion during surgery and no specific diagnostic modality has yet been defined for the detection of AT (4). The white blood cell (WBC) count increases during inflammatory processes in the body. It was shown that WBC count increases in cases of AT (5). Many studies have shown that the neutrophil-to-lymphocyte ratio (NLR) is a significant inflammatory marker in various diseases (6,7). The mean platelet (PLT) volume (MPV) is also important for the diagnosis of inflammatory states. The diagnostic importance of MPV has been shown in diseases such as acute appendicitis, pelvic inflammatory, and ectopic pregnancy (7-9). Patients with pelvic pain due to adnexal causes should be diagnosed carefully. Ruptured or unruptured ovarian cysts (UOC) may cause pelvic pain. Clinical conditions requiring surgery should be differentiated from follow-up patients. The aim of this study was to investigate the diagnostic value of the NLR in the differential diagnosis of AT with ruptured or UOC.

Materials and Methods

Patients and data

A database of the 80 patients who underwent surgery for AT and ovarian cysts between January 2012 and June 2017 was retrospectively investigated. Patients with a suspicion of malignancy and tubal ovarian abscess were excluded from the study because malignancies and abscess formation may have an effect on blood count parameters. Three groups were formed [AT (n=35), UOC (n=25), and ovarian cyst rupture (OCR) (n=20)] and investigated. Demographic features, WBCs, neutrophil, lymphocyte, and PLT counts and hemoglobin (Hb) levels, NLR, MPV and red cell distribution width (RDW) before surgery were recorded. This study was approved by the Iocal Research Ethics Committee of Marmara University Faculty of Medicine the Institution (date: 09.2017 approval number: 543).

Statistical Analysis

Statistical analyses were performed using the SPSS 20.0 (SPSS, Version 20.0; Chicago, IL, USA) statistics software. In the study, descriptive and categorical data were evaluated as number (n) and percentage (%), and continuous data were studied as interquartile range and medians. The Mann-Whitney U test and Kruskal Wallis test were used for comparisons. The significance level was accepted as p<0.05.

Results

Demographics

The demographic findings of the groups were evaluated (Table 1). The median ages of the AT OCR, and UOC groups were 24, 32.5, and 34 years, respectively. There was a significant difference in respect to age and parity status of the patients. This difference was an expected feature of the AT cases, which are mostly seen at younger ages.

Complete Blood Counts

The median WBC count for the AT group was 12.1x[10.sup.3]/[micro]L, OCR was 12.0x[10.sup.3]/[micro]L, and the UOC was 7.3x[10.sup.3]/[micro]L. There was a statistically significant difference between the groups (p<0.001). When the AT group was compared with the other groups individually, the AT group had no significant difference with the OCR group but had a significant difference with the UOC group (p=0.0834 and p<0.001). The median Hb levels of the AT, OCR, and UOC groups were 11.6 g/dL, 12.0 g/dL, and 12.5 g/dL (p<0.001). When further analysis was conducted, the statistically significant difference was due to the difference between the AT group and the UOC (p<0.001); no significant difference was noted between the AT and OCR groups. The median neutrophil counts for groups were found as 9.9x[10.sup.3]/[micro]L for the AT group, 9.9x[10.sup.3]/[micro]L for the OCR group, and 4.7x[10.sup.3]/[micro]L for the UOC group. The significant difference was attributable to the difference between the AT group and UOC group, rather than the difference between the AT and OCR groups (p<0.001 and p=0.986). The median lymphocyte count was 1.3x[10.sup.3]/[micro]L for the AT group, 1.4x[10.sup.3]/[micro]L for the OCR group, and 2.0x[10.sup.3]/[micro]L for the UOC group. When the AT group was compared with the OCR group, no statistically significant difference was found regarding lymphocyte counts (p=0.273), whereas there was a statistically significant difference between the AT and the UOC groups (p=0.009). The median RDWs, PLTs, and MPVs for all groups showed no statistically significant difference. The NLR was found to have a difference that reached statistical significance (p<0.001). When the groups were individually compared with the AT group, NLR was not found to be different to the OCR group (p=0.372), but there were a significant difference with the UOC group (p<0.001). Table 2 shows the complete blood count results and NLR of the three groups.

Discussion

AT has a frequency of 2.5 to 7.4% in emergency gynecologic operations. It is common in reproductive-age women and early diagnosis of this condition is vital for the preservation of the vitality of the ovary (10). AT has nonspecific symptoms such as aches, nausea, vomiting, and low-grade fever, and as such, it is difficult to diagnose. OCR, ectopic pregnancy, adnexitis, acute appendicitis, gastroenteritis, and renal colic should be differentiated from AT (11). The diagnosis of AT is generally based on ultrasonography. An increase in ovarian size, cyst of the adnexa, and free liquid in the pelvic cavity are the findings in ultrasound. However, ultrasound was shown to be normal in nearly half of all AT cases, especially in children (12). Another diagnostic method is color doppler ultrasound, which is widely used for diagnosis of AT; doppler flow absence or decrease in ultrasound favors the diagnosis of AT. The detection of a normal blood flow pattern due to the dual blood supply of ovaries in 60% of cases of AT makes the role of doppler ultrasound in preoperative diagnosis debatable (13,14).

NLR has been a popular parameter for the diagnosis of several inflammatory and surgical conditions in which it has been shown to be superior to WBC counts (15). NLR has also been shown to be useful in the diagnosis of many diseases concerning gynecologic inflammatory disorders and malignancies in several studies (16-18).

Pelvic pain requiring surgery due to adnexal pathologies may sometimes be a confusing issue for surgeons, especially in cases of OCR, which can be clinically followed up without a surgical intervention. Therefore, we aimed to investigate the role of NLR for the differential diagnosis of AT from OCR and UOC in this study. Ercan et al. (19) compared preoperative NLR and WBC counts in patients with AT and ovarian cysts. They found an increase in both WBC and neutrophil counts in both groups, but a lower lymphocyte count in the ovarian cyst group. NLR was found to be significantly higher in the AT group compared with the ovarian cyst group (19). We also found a significant difference in both WBC and neutrophil and lymphocyte counts and NLR of AT, OCR, and UOC. When the groups were individually compared, it was found that there was no significant difference between the OCR group and the AT group, whereas a significant difference was found between the AT group and the UOC group. In a similar study, AT and ovarian cyst groups were compared with several complete blood count markers. NLR, WBC, and neutrophil counts were found to be higher in the AT group compared with the ovarian cyst group. We also found the same difference, but as above, it was due to the UOC and AT group difference rather than the UOC and AT group difference. MPV and platelet counts were also investigated in the same study and no difference was found between the groups, similar to our findings (20). OCR may need surgical intervention but can also be followed up clinically in hemodynamically stable cases. Observation with analgesia can usually be used for the management of hemorrhagic cysts and cyst rupture. Surgery should be performed in cases in which there is hemodynamic compromise, diagnostic uncertainty or suspicion of torsion, no relief of symptoms within 48 hours, and an increase in hemoperitoneum, and a decrease in HB level is detected (21).

Ruptured cysts and AT are different in respect to management, clinical follow-up of a ruptured cyst may be useful in specific cases, but early surgical intervention is important for preserving fertility in AT. NLR has been shown to be useful in differentiating ovarian cysts and AT (19,20). Contrary to those findings, we found that NLR was not useful in the differential diagnosis of OCR and AT cases.

Study Limitations

The retrospective nature of the study is a limiting factor. Only data of patients of a single center were collected. Number of cases should be increased for to reach a clearer conclusion about the diagnostic value of neutrophil to lymphocyte ratio in differential diagnosis of adnexal torsion and ovarian cysts.

Conclusion

NLR can be used in the preoperative differential diagnosis of ovarian cysts and AT. However, the diagnostic value of NLR in the differentiation of OCR and AT cases was not determined in this study.

Ethics

Ethics Committee Approval: This study was approved by the Marmara University Local Ethics Committee (approval number: 543 date: 09.2017).

Informed Consent: Consent form was filled out by all participants.

Peer-review: External and internal peer-reviewed.

Authorship Contributions

Surgical and Medical Practices: S.S., R.B.B., Concept: S.S., Design: S.S., Data Collection or Processing: S.S., R.B.B., Analysis or Interpretation: S.S., R.B.B., Literature Search: S.S., Writing: S. S.

Conflict of Interest: No conflict of interest was declared by the authors.

Financial Disclosure: The authors declared that this study received no financial support.

References

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(2.) Huchon C, Fauconnier A. Adnexal torsion: a literature review. Eur J Obstet Gynecol Reprod Biol 2010;150:8-12.

(3.) White M, Stella J. Ovarian torsion: 10 year perspective. Emerg Med Australas 2005;17:231-7.

(4.) Bar-On S, Mashiach R, Stockheim D, Soriano D, Goldenberg M, Schiff E, et al. Emergency laparoscopy for suspected ovarian torsion: are we too hasty to operate? Fertil Steril 2010;93:2012-5.

(5.) Tobiume T, Shiota M, Umemoto M, Kotani Y, Hoshiai H. Predictive factors for ovarian necrosis in torsion of ovarian tumor. Tohoku J Exp Med 2011;225:211-4.

(6.) Arbel Y, Finkelstein A, Halkin A, Birati EY, Revivo M, Zuzut M, et al. Neutrophil/lymphocyte ratio is related to the severity of coronary artery disease and clinical outcome in patients undergoing angiography. Atherosclerosis 2012;225:456-60.

(7.) Yardimci S Ugurlu MU, Coskun M, Attaallah W, Yegen SC. Neutrophil-lymphocyte ratio and mean platelet volume can be a predictor for severity of acute appendicitis. Ulus Travma Acil Cerrahi Derg 2016;22:163-8.

(8.) Incebiyik A, Seker A, Vural M, Gul Hilali N, Camuzcuoglu A, Camuzcuoglu H. May mean platelet volume levels be a predictor in the diagnosis of pelvic inflammatory disease? Wien Klin Wochenschr 2014;126:422-6.

(9.) Artunc Ulkumen B, Pala HG, Calik E, Oruc Koltan S. Can mean platelet volume and platelet distrubition width be possible markers for ectopic pregnancy and tubal rupture?(MPV and PDW in ectopic pregnancy). Pak J Med Sci 2014;30:352-5.

(10.) Houry D, Abbott JT. Ovarian torsion: a fifteen-year review. Ann Emerg Med 2001;38:156-9.

(11.) Nur Azurah AG, Zainol Z W, Zainuddin AA, Lim PS, Sulaiman AS, Ng BK. Update on the management of ovarian torsion in children and adolescents. World J Pediatr 2015;11:35-40.

(12.) Oltmann SC, Fischer A, Barber R, Huang R, Hicks B, Garcia N. Cannot exclude torsion--a 15-year review. J Pediatr Surg 2009;44:1212-6.

(13.) Mage G, Canis M, Manhes H, Pouly JL, Bruhat MA. Laparoscopic management of adnexal torsion. A review of 35 cases. J Reprod Med 1989;34:520-4.

(14.) Servaes S, Zurakowski D, Laufer MR, Feins N, Chow JS. Sonographic findings of ovarian torsion in children. Pediatr Radiol 2007;37:446-51.

(15.) Zahorec R. Ratio of neutrophil to lymphocyte counts-rapid and simple parameter of systemic inflammation and stress in critically ill. Bratisl Lek Listy 2001;102:5-14.

(16.) Haruma T, Nakamura K, Nishida T, Ogawa C, Kusumoto T, Seki N, et al. Pre-treatment neutrophil to lymphocyte ratio is a predictor of prognosis in endometrial cancer. Anticancer Res 2015;35:337-43.

(17.) Akopuz A, Turan V, Ozcan A, Kopuz Y, Toz E, Kurt S. A novel marker for the assessment of the treatment result in pelvic inflammatory disease. Minerva Ginecol 2016;68:117-23.

(18.) Yildirim MA, Seckin K D, Togrul C, Baser E, Karsli M F, Gungor T, et al. Roles of neutrophil/lymphocyte and platelet/lymphocyte ratios in the early diagnosis of malignant ovarian masses. Asian Pac J Cancer Prev 2014;15:6881-5.

(19.) Ercan O, Kostu B, Bakacak M, Coskun B, Tohma A, Mavigok E. Neutrophil to Lymphocyte ratio in the diagnosis of adnexal torsion. Int J Clin Exp Med 2015;8:16095-100.

(20.) Koleli I. Mean platelet volume in early diagnosis of adnexal torsion. Balkan Med J 2015;32:410-3.

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[iD] Sunullah Soysal, [iD] Rezzan Berna Baki

Marmara University Faculty of Medicine, Department of Obstetrics and Gynecology, Istanbul Turkey

Address for Correspondence/Yazisma Adresi: Sunullah Soysal, MD,

Marmara University Faculty of Medicine, Department of Obstetrics and Gynecology, Istanbul Turkey

Phone: +90 505 795 95 57 E-mail: drsunullah@yahoo.com ORCID ID: orcid.org/0000-0002-1055-6585

Received/Gelis Tarihi: 05.04.2018 Accepted/Kabul Tarihi: 26.04.2018

[c]Copyright 2018 by Turkish Society of Obstetrics and Gynecology

Turkish Journal of Obstetrics and Gynecology published by Galenos Publishing House

DOI: 10.4274/tjod.95881
Table 1. Demographic findings

                 AT                           OCR
         Median  Interquartile range  Median  Interquartile range

Age      24      19-31                32.5    28-37
Gravida   0       0-1                  1       0-2
Parity    0       0-1                  1       0-2

                 UOC                  p
         Median  Interquartile range

Age      34      29-46                <0.001
Gravida   1       0-4                  0.08
Parity    1       0-2                  0.03

AT: Adnexal torsion, OCR: Ovarian cyst rupture, UOC: Unruptured ovarian
cysts

Table 2. Complete blood count parameters and neutrophil-to-lymphocyte
ratio

                                    AT                     OCR
                            Median  Interquartile  Median  Interquartile
                                    range                  range

WBC (x[10.sup.3]/[micro]L)   12.1     9.8-13.3      12.0     7.9-14.8
HB (g/dL)                    11.6    10.8-12.5      10.7     9.6-12.3
N (x[10.sup.3]/[micro]L)      9.9     7.9-11.4       9.9     4.9-12.4
RDW                          13.4    12.8-14.4      14.6    13.4-15.5
PLT (x[10.sup.3]/[micro]L)  262     206-334        218.5   190.5-286
MPV (fL)                      8.9     7.7-9.3        9.0     8.3-9.4
L (x[10.sup.3]/[micro]L)      1.3     0.8-1.9        1.4     1.0-1.8
NLR                           8.0     4.0-14.1       7.5     3.7-11.5

                                    UOC
                            Median  Interquartile  p
                                    range

WBC (x[10.sup.3]/[micro]L)    7.3     5.8-9.1      <0.001
HB (g/dL)                    12.5    11.5-13.4      0.04
N (x[10.sup.3]/[micro]L)      4.7     3.6-5.8      <0.001
RDW                          13.4    13.0-14.1      0.126
PLT (x[10.sup.3]/[micro]L)  258     211-284         0.157
MPV (fL)                      8.5     7.9-8.9       0.243
L (x[10.sup.3]/[micro]L)      2.0     1.6-2.4       0.016
NLR                           2.2     1.8-2.7      <0.001

WBC: White blood cell, HB: Hemoglobin, N: Neutrophil, RDW: Red cell
distribution width, PLT: Platelet, MPV: Mean platelet volume, L:
Lymphocyte; NLR: Neutrophil-to-lymphocyte ratio, AT: Adnexal torsion,
OCR: Ovarian cyst rupture, UOC: Unruptured ovarian cysts
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Article Details
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Title Annotation:Clinical Investigation / Arastirma
Author:Soysal, Sunullah; Baki, Rezzan Berna
Publication:Turkish Journal of Obstetrics and Gynecology
Article Type:Report
Date:Jun 1, 2018
Words:2740
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