Printer Friendly

Developmental mode in opisthobranch molluscs from the northeast Pacific Ocean: additional species from Southern California and supplemental data.

Abstract.--We document development type for 33 species of benthic opisthobranch gastropods 15 for the first time--collected mainly from the Southern California Bight. Fourteen of the newly examined species had planktotrophic development, while the dorid nudibranch Atagema alba had capsular metamorphic development, the first example of direct development in a non-dendrodoridid nudibranch known from the northeast Pacific Ocean. For the remaining 18 species our new data are either consistent with earlier determinations of development type, or confirm previous inferences. The new data also broaden geographic coverage for some species, and for the sacoglossan Stiliger fuscovittatus and the nudibranch Melibe leonina, suggest that egg size is inversely related to temperature. We correct the previous erroneous identification of nephrocysts as eyespots in the hatching planktotrophic larvae of the nudibranchs Tritonia festiva and Janolus fuscus. These results further highlight the predominance of planktotrophic development in benthic opisthobranchs from the northeast Pacific Ocean.

Introduction

Mode of development has been determined for approximately two-thirds of the over 300 species of opisthobranch molluscs known from the northeast Pacific Ocean (Goddard 2004, 2005; Goddard and Hermosillo 2008). Small eggs and planktotrophic development predominate among these species; so far, only 14 species from the region are known to hatch from their egg coverings as either lecithotrophic larvae or juveniles (Goddard and Hermosillo 2008). The four species of nudibranchs known to bypass a free-living larval stage and hatch as juveniles all belong to a single family, the Dendrodorididae (Goddard, 2005).

Developmental mode in opisthobranchs is most reliably documented when based on observations of the morphology of hatching veliger larvae--particularly the presence or absence of eyespots and propodium--accompanied by measurements of egg size, embryonic period and temperature, and size and type of shell at hatching (Bonar 1978; Goddard 2004). However, these parameters are not always readily measured or reported in the literature, and developmental mode has previously been inferred for some species based on subsets of the above information. For example, Goddard (2004) characterized as planktotrophic the development of the nudibranchs Polycera tricolor Robilliard, 1971 and Dirona picta MacFarland in Cockerell & Eliot, 1905, based on, respectively, (1) a comparison of the embryonic period reported for P. tricolor by Robilliard (1971) with those of congeners of known developmental type, and (2), qualitative observations, combined with a measurement of the preserved embryos reported by Marcus and Marcus (1967). As opportunities have arisen, we have endeavored to fill some of these gaps in the available embryological data, as well as sample across more of the geographic range of some species. Aside from providing confirmation or not of previous inferences, this will enable more robust comparative studies, help distinguish closely related species, and increase our understanding of geographic variation in the life history traits of opisthobranchs.

Here we document mode of development for species not previously examined, most of which are from southern California, and fill existing gaps in the embryological data for others. We include species rarely observed or mainly subtidal in distribution, two undescribed species, and document a new example of rare direct development in a nudibranch from the northeast Pacific Ocean. We discuss discrepancies between our observations and those reported in the literature and also correct the previous erroneous identification by the senior author of the paired larval structures known as nephrocysts (e.g., Thompson 1976; Bonar 1978) as eyespots in the hatching larvae of two common planktotrophic species.

Collection Sites and Methods

Adult opisthobranchs, or portions of their egg masses in the process of being deposited, were collected by hand from subtidal and intertidal sites along the Pacific coast of North America (Table 1). Adults were held in containers (250 to 1000 ml) of unfiltered seawater at near ambient ocean temperatures until they laid egg masses. Recently laid egg masses were examined using a compound microscope equipped with an ocular micrometer. If first cleavage had not commenced, the diameters of a random sample of 10 zygotes were measured in each egg mass; otherwise, an upper limit on zygote size was estimated by measuring the dimensions of a few randomly selected embryos at or before the gastrula stage. We then isolated individual egg masses, or approximately 1 cm long sections of larger egg masses, into separate, labeled vials (20 ml) and changed and gently swirled the seawater in these once or twice daily. We examined the egg masses daily until hatching and then measured the longest dimension of their shells and assigned mode of development (planktotrophic, lecithotrophic or direct) and larval shell type (coiled type 1 or egg-shaped, inflated type 2) as in Goddard (2004). After obtaining the above egg masses, adult specimens were relaxed in 7.5% Mg[Cl.sub.2], fixed in 70% ethanol, and deposited as voucher specimens in the California Academy of Sciences. Some of these vouchers included pieces of egg masses and were supplemented by digital images of adults. We used an underwater data logger (Stow Away Tidbit, Onset Computer Corp.) to record temperature at 10 min. intervals in our holding containers.

For the dorids Geitodoris mavis Marcus & Marcus, 1967 and Taringa aivica Marcus & Marcus, 1967, we examined preserved specimens originally collected in 1994 and 2000, respectively, and following Goddard (2004), inferred their mode of development based on the relatively small size of their embryos. For the chromodorid Felimare californiensis (= Hypselodoris californiensis) (Bergh, 1879) we inferred mode of development based on estimates of embryonic period and egg size. We estimated the latter by measuring the zygotes in an image of an adult laying its egg ribbon (Figure 2A) and assuming an egg ribbon width of 6 mm (adjusted to 4.243 mm to account for the approximately 45[degrees] viewing angle of the segment of the egg ribbon containing the zygotes we measured), based on the measurement reported by Ingrain (1935). Craig Hoover (personal communication to JG, 30 Sept 2012) provided information on the embryonic period of F. californiensis, based on subtidal observations he made in September 2012 of three egg masses laid by F. californiensis in Big Fisherman Cove, Santa Catalina Island.

JG identified the egg mass of Atagema alba (O'Donoghue, 1927) from Bird Rock, La Jolla (see Table 2) based on original 35 mm slides, including close-ups of sections of egg ribbon, in the James R. Lance collection at the California Academy of Sciences (CAS) in San Francisco.

We follow the taxonomic nomenclature of Behrens and Hermosillo (2005), and for the Chromodoridae, Johnson and Gosliner (2012).

Results

We obtained results on the development of 33 species, 15 for the first time (Table 2). Eleven of these 15 laid small eggs and developed into hatching planktotrophic larvae (Table 2). Planktotrophic development was inferred for an additional three of them (Geitodoris mavis, Felimare californiensis, and Flabellina pricei), based on the small size of their embryos, and for Felimare califoniensis, the length of its embryonic period as well (Table 2). Finally, Atagema alha from La Jolla developed from relatively large eggs and had capsular metamorphic development (Table 2, Figure 1). The juveniles of this species exited their shells while leaving the egg capsules, and one day after hatching had rhinophore buds and an oval notum reinforced by calcareous spicules and edged with secretory glands (Figure 1B & C). Five days after hatching, the juveniles had two to three notal caryophyllidia (papillae surrounded by crowns of spicules; Figure 1D). A radula with three pairs of hammate teeth was visible two days after hatching (Figure 1 F) and appeared to have approximately six rows of teeth after seven days (Figure 1E).

Of the remaining 18 species examined, hatching planktotrophic larvae were observed in 15, and this same mode of development was inferred for the remaining three based on the small size of their embryos (Table 2).

Discussion

Thirty-two of the 33 total species we examined were found to have planktotrophic development, consistent with prior compilations of developmental mode in benthic opisthobranchs from both the temperate and tropical northeast Pacific Ocean (Goddard 2004; Goddard and Hermosillo 2008). Atagema alba, with capsular metamorphic development, was the only species we observed in this study with non-feeding development. It represents the first example of direct development in a non-dendrodoridid nudibranch from the region. For each of the 18 species previously examined, including Polycera tricolor and Dirona picta, our new data either support earlier determinations of development type, or confirm previous inferences (see Goddard 2004, Table 1). Below we (1) discuss individual species, including discrepancies with previously reported embryological data, and (2) correct the previous erroneous identification by one of us (JG) of nephrocysts as eyespots in two species.

Stiliger fuscovittatus Lance, 1962

Case (1972) studied this diminutive sacoglossan in San Francisco Bay and reported an uncleaved egg diameter of 70 [micro]m and shell-size at hatching of 110 [micro]m, similar to the values reported here for specimens from San Diego and Monterey. In contrast, Strathmann (1987) reported values of 95 [micro]m and 150 [micro]m for eggs and hatching larvae, respectively, from specimens from the San Juan Islands, Washington. A similar size discrepancy exists between our values for the eggs and larvae of Melibe leonina (Gould, 1852) from Santa Barbara (Table 2) and those reported by Strathmann (1987) for this species from the Washington and British Columbia. These size discrepancies are consistent with the inverse relationship between temperature and egg and larval sizes known for some marine invertebrates (discussed by Moran & McAlister 2009), and might be worth investigating experimentally.

Atagema alba (O'Donoghue, 1927)

The egg mass of this species was found under an intertidal cobble at Bird Rock, La Jolla. The egg mass was a fairly stout ribbon 3 mm high, coiled in 1.3 turns, with a simple (not wavy) free edge and a total diameter of 6.5 mm. The zygotes and embryos were white. Compared to the images in the Lance collection at CAS of an egg mass laid in captivity by an A. alba collected intertidally in San Diego in July 1969, the egg mass we found had one less turn, but was otherwise virtually identical in appearance, including the relatively large size and spacing of the embryos. In the northeast Pacific Ocean, only Dendrodoris behrensi Millen & Bertsch, 2005 deposits a similar egg mass, but its embryos are significantly larger than those of A. alba, are deposited in thicker-walled capsules, and undergo ametamorphic direct development (Goddard 2005). Our voucher specimens (CASIZ 186493) of A. alba consist of post-metamorphic juveniles as pictured in Figure 1D.

Doris pickensi Marcus & Marcus, 1967

This species, previously known only from the northern Gulf of California to Costa Rica (Behrens and Hermosillo 2005; Camacho-Garcia et al. 2005), laid its egg ribbon flat, rather than on edge like most other dorids. The yellow ribbon, pictured in Goddard (2012a), measured up to 2.3 mm wide and was laid in a loose coil of three turns.

Felimare californiensis (Bergh, 1879)

Our estimate of 100 [micro]m for the egg diameter of this species is based on Ingram's (1935) measurement of a single egg ribbon, applied to Figure 2A, and therefore depends on variability in egg ribbon width in F. californiensis, which is unknown. However, the estimate is similar to the 95 [micro]m egg size of another chromodorid, the sympatric Felimida macfarlandi (Cockerell, 1902), a known planktotroph (Goddard 2004), as well as planktotrophic representatives in the northeastern Pacific Ocean of the closely related genus Cadlina (Goddard 2004).

Based on in situ observations by Craig Hoover of three egg masses in Big Fisherman Cove, Santa Catalina Island, F. californiensis developed to hatching at 17-19 [degrees]C in at least seven days, but not more than 12 days (Table 2). At these temperatures, this range in embryonic period falls within that typical of dorid nudibranchs with similarly sized eggs and planktotrophic development and is also at least a week shorter than embryonic periods known for species with non-feeding modes of development in the NE Pacific Ocean (Hadfield and Switzer-Dunlap 1987; Goddard 1996; Goddard and Hermosillo 2008). We therefore conclude that F. californiensis has planktotrophic development. Based on an image in (Kopp 2008), a smaller congener of F. californiensis, F. porterae (Cockerell, 1901) also lays relatively small eggs and likely has planktotrophic development.

Although the information we present here on the development of F. californiensis is incomplete and less precise than for other species, we have included it to help assess the cause of the loss of this species from the mainland of southern California. Felimare californiensis was once common throughout the Southern California Bight, but in contrast to other Californian nudibranchs in the region, has not been sighted on the mainland in over three decades (M. Miller in Behrens 2001; Goddard et al. 2013).

Palio dubia (M. Sars, 1829)

The size of the embryos from Alaska is consistent with measurements of the eggs and embryos of this species from the North Atlantic Ocean (Hamel et al. 2008; Goddard 2011a).

Triopha maculata MacFarland, 1905

Mulliner (1972) reported that an 80 mm long Triopha grandis MacFarland 1966 "produced 400,000 eggs per egg-mass" and hatched after 6 days (at an unspecified temperature) into "free swimming, planktotrophic veligers." Because both the large brood size and short hatching time are entirely consistent with planktotrophic development, Goddard (2004) followed Mulliner's (1972) determination of developmental mode for this species, which was synonymized, along with Triopha oecidentalis (Fewkes, 1889), with T. maculata by Ferreira (1977). Behrens (1991) and Goddard (2004) followed Ferreira (1977), but not McDonald (1983, 2007), who considers T. grandis a junior synonym of T. occidentalis, separate from T. maculata. Our finding that the eggs deposited by a 50 mm long T. maculata were encapsulated singly (Table 2) contrasts with Mulliner's (1972) report of an average of 18 eggs per capsule for T. grandis. This large difference between egg masses in the number of eggs per capsule suggests that T. grandis may indeed be separate from T. maculata, as argued by McDonald (1983, 2007).

Doto columbiana O'Donoghue, 1921

This species feeds on hydroids of the genus Aglaophenia and has previously been referred to by the senior author as Doto form B (Goddard, 1996, 2004). The sizes of the eggs and hatching larvae reported here from Hazard Canyon are similar to those reported from northern California by Goddard (1996).

Janolus anulatus Camacho-Garcia & Gosliner, 2006

The cream to white egg strings of this species (see Goddard 2012b) contained embryos encapsulated singly. This contrasts with other species of Janolus known from the northeast Pacific Ocean, including J. barbarensis (Cooper, 1863), with 15 to 20 embryos per capsule (present study, Table 2), and J. fuscus O'Donoghue, 1924, with an average of 66 embryos per capsule (Wolf and Young 2012). The zygotes and hatching larvae of J. anulatus were also significantly smaller than those of the above two congeners (Goddard 2004; Wolf and Young 2012; present study, Table 2).

Cumanotus sp.

Following Behrens (1991), Goddard (2004) referred to this species, which feeds on Ectopleura crocea (Agassiz, 1862) in bays and harbors, as Cumanotusfernaldi Thompson & Brown, 1984. However, as pointed out by S. Millen (personal communication to JG, 8 Jan 2012), Thompson and Brown (1984) in their brief description of C. fernaldi were referring to the larger, soft-sediment dwelling species studied by Hurst (1967) that is ecologically and morphologically similar to the north Atlantic C. beaumonti (Eliot, 1906). Hurst (1967) used the name C. beaumonti for her specimens from Washington and reported that they laid corkscrew shaped egg masses with 4-14 eggs per egg capsule. Cumanotus sp. consistently has one egg per capsule (Goddard 1992; present study, Table 2), is ecologically and morphologically similar to the north Atlantic C. cuenoti Pruvot-Fol, 1948, and is undescribed (S. Millen, personal communication to JG, 8 Jan 2012). It is pictured as C. fernaldi in Behrens and Hermosillo (2005, species number 244), but that name actually applies to species number 245 in Behrens and Hermosillo (2005). (S. Millen, personal communication to JG, 8 Jan 2012).

Unaware that two species of Cumanotus exist in the northeast Pacific Ocean, Goddard (2004, Table 1) lumped development data for both species. The complete and correct breakdown is as follows. Based on Hurst (1967), C. fernaldi (as C. beaumonti) deposits corkscrew-shaped egg masses with 4-14 eggs per capsule that hatch after 10 days at 8-11 [degrees]C with type 1 shells averaging 119 [micro]m long. Based on Goddard (1992, 2011b) and the present study, Cumanotus sp. also deposits corkscrew-shaped egg masses, but with one egg (averaging 73 [micro]m in diameter) per capsule, hatching after 9-10 days at 12-16 [degrees]C with type 1 shells averaging 130 [micro]n long.

Cuthona lagunae (O'Donoghue, 1926)

The egg masses and embryonic development of specimens of C. lagunae collected from Hazard Canyon in central California were virtually identical to those described by Goddard (1991) for specimens from northern California and southern Oregon, including the extrusion from the zygotes of unusually large, yolk-filled polar bodies.

Eubranchus sp, 2 of Behrens and Hermosillo (2005)

This species laid small, C-shaped egg masses characteristic of the genus. The embryos developed into planktotrophic veligers with type 2 shells, a small foot, eyespots, and minimal yolk reserves (Figure 2B).

Flabellina goddardi Gosliner, 2010

The developmental data for this species in Table 2 were obtained from the coiled and secondarily looped, white egg string and near-hatching veligers pictured without size or scale information in Gosliner's (2010) original description of this distinctive species. Since finding the first specimen in May 2008, we have found 14 additional specimens of F. goddardi, all in May and June at Tarpits Reef in Carpinteria, California, the type locality of this species.

Flabellina trilineata (O'Donoghue, 1921)

Two specimens found feeding on Ectopleura crocea in the Santa Barbara Harbor in January 2012 each laid egg strings with most capsules containing 2 eggs (Table 2). Previously, only one egg per capsule has been recorded for this species, including for specimens from the outer coast of Santa Barbara County (Bridges and Blake 1972; Strathmann 1987; Goddard 1992; present study).

Flabellina sp.

Adults were collected intertidally at Naples, on the south coast of Santa Barbara County. They closely resembled F. trilineata collected from the same locality in overall shape, but had smooth to slightly wrinkled rhinophores, more irregular white lines on the body, and white cnidosacs. Instead of a thin white line down the middle of the notum as in F. trilineata, some of our specimens of Flabellina sp. had a notum mostly covered with white pigment. Flabellina sp. deposited egg masses similar to those of F. trilineata collected from the same locality, but the ribbons of Flabellina sp. had a smaller diameter. The eggs were similar in diameter to those of F. trilineata, but the shells of the hatching veligers were slightly larger (Table 2). Preliminary genetic evidence suggests Flabellina sp. is distinct from F. trilineata (R. Johnson, personal communication to JG, 23 June 2010).

In his description of Coryphella piunca, Marcus (1961, Fig. 163) illustrated smooth to lamellate rhinophores. Steinberg (1963) synonymized C. piunca with C. trilineata O'Donoghue, 1921, and later, Roller (1970) synonymized C. fisheri MacFarland, 1966 with C. trilineata. Because both MacFarland's (1966) description of C. fisheri and O'Donoghue's description of C. trilineata only mention annulate or lamellate rhinophores, it appears that Marcus's C. piunca likely included specimens of both F. trilineata and Flabellina sp. as recognized here.

Goddard (1984, Table 1) reported that dendronotid Tritonia Jestiva has eyespots at hatching, and Strathmann (1987, p. 291) and Goddard (2001 b, Appendix A) repeated this. Reexamination of JG's original notes from 1981 and photomicrographs taken by JG in 1987 of the hatching larvae of this species revealed the paired structures were not eyespots, but rather nephrocysts (see Thompson 1976; Bonar 1978; Bickell and Kempf 1983) containing unusually large and dense, irregularly shaped, semi-translucent granules. At low magnification and certain angles of view and illumination, these structures can appear to be eyespots (Figure 2C). However, larval eyespots in opisthobranchs consist of thin patches of opaque black pigment, develop an overlying hyaline lens, and are located anterior to the nephrocysts (Thompson 1976; Bonar 1978). Goddard (1992, 2001b) also mistook dense nephrocysts for eyespots in the arminid nudibranch Janolus fuscus, but these have also been confirmed to be nephrocysts in both this species (Wolf and Young 2012; pers. obs.), as well as its congener, J. anulatus (present study, Figure 2D).

The combined presence of eyespots and a propodium is a reliable indicator of metamorphic competence in opisthobranchs (Bonar 1978), and most planktotrophic nudibranchs hatch without eyespots (Bonar 1978; Hadfield and Switzer-Dunlap 1984; Goddard 2004). However, exceptions are known from the northeast Pacific Ocean, including species of Eubranchidae and Tergipedidae (Hurst 1967; Strathmann 1987; Goddard 1991 ; present study, Figure 2B) and the dorid Aegires albopunctatus MacFarland, 1905, which has unusual larval development (Goddard 2001a).

Acknowledgements

BG thanks the captain and crew of the Truth Aquatics dive boat Vision, as well as John Yasaki, Brynn Hooton, Terry Strait, Kim Mitchell and Brian Swift. We also thank Jack Engle for permission to use his image of Felimare californiensis, Maya Wolf for sharing with us her fine images of the hatching larvae of Janolus fuscus, and Craig Hoover for sharing his in situ observations on the egg masses of Felimare californiensis. Two anonymous reviewers commented on the manuscript, and we are thankful for their efforts. Some of the specimens documented in this study were collected during a study of intertidal nudibranchs funded by grant R/OPCENV-08 from the California Ocean Protection Council and California Sea Grant to JHRG.

References

Behrens, D.W. 1991. Pacific coast nudibranchs. Sea Challengers: Monterey, Calif.. vi+107 pp. --. 2001. Hypselodoris californiensis (Bergh, 1879). Available from: http://slugsite.us/bow/nudwk255.htm. Accessed 15 Jan 2012.

-- and A. Hermosillo. 2005. Eastern Pacific nudibranchs. Sea Challengers, Monterey, Calif. vi+137 pp.

Bickell, L.R. and S.C. Kempf. 1983. Larval and metamorphic morphogenesis in the nudibranch Melibe leonina (Mollusca: Opisthobranchia). Biol. Bull., 165:119 138.

Bonar, D.B. 1978. Morphogenesis at metamorphosis in opisthobranch molluscs. Pp. 177-196 in F.S. Chia and M.E. Rice (eds.), Settlement and metamorphosis of marine invertebrate larvae. Elsevier: New York. xi+290 pp.

Bridges, C. and J.A. Blake. 1972. Embryology and larval development of Coryphella trilineata O'Donoghue, 1921 (Gastropoda: Nudibranchia). Veliger, 14:293 297.

Camacho-Garcia, Y., T.M. Gosliner, and A. Vald6s. 2005. Field guide to the sea slugs of the tropical eastern Pacific. California Academy of Sciences: San Francisco, Calif., 129 pp.

Case, R.M. 1972. An ecological study of Stiliger fuscovittata (Mollusca: Opisthobranchia). M.A. Thesis, California State University, San Francisco, 76 pp.

Ferreira, A.J. 1977. A review of the genus Triopha (Mollusca: Nudibranchia). Veliger, 19:387-402.

Goddard, J.H.R. 1984. The opisthobranchs of Cape Arago, Oregon, with notes on their biology and a summary of benthic opisthobranchs known from Oregon. Veliger, 27:143 163.

--. 1991. Unusually large polar bodies in an aeolid nudibranch: a novel mechanism for producing extra-embryonic yolk reserves* J. Moll. Stud., 57(4)(Suppl.): 143-152.

--. 1992. Patterns of development in nudibranch molluscs from the northeast Pacific Ocean, with regional comparisons. Ph.D. thesis, University of Oregon, Eugene, Oregon., 237 pp.

--. 1996. Lecithotrophic development in Doto amyra (Nudibranchia: Dendronotacea), with a review of developmental mode in the genus. Veliger, 39:43-54.

--. 2001a. The early veliger larvae of Aegires albopunctatus (Nudibranchia: Notodorididae), with morphological comparisons to members of the Notaspidea. Veliger, 44:398-400.

--. Mollusca: Gastropoda. Pp. 86 128 in A.L. Shanks (ed.), An identification guide to the larval marine invertebrates of the Pacific Northwest. Oregon State University Press: Corvallis, Oregon, 314 pp.

--. 2004. Developmental mode in opisthobranch molluscs from the northeast Pacific Ocean: feeding in a sea of plenty* Can. J. Zool., 82:1954-1968.

--. 2005. Ametamorphic direct development in Dendrodoris behrensi (Nudibranchia: Dendrodorididae), with a review of developmental mode in the family. Proceed. Calif. Acad. Sci., 56:201 211.

--. 2011a. Palio dubia (Nudibranchia: Doridina) from the north-west Atlantic Ocean: is its morphology at hatching consistent with settlement one day later? J. Mar. Biol. Assoc. U.K., 91: 1651 1654.

--. 2011b. Cumanotus feeding on Ectopleura crocea. Available from: http:/lwww.flickr.com/photos/34486353@NO7/65922598711in/set-72157612784841131. Accessed 18 Dec 2012.

--. 2012a. Doris pickensi laying eggs. Available from: http:/lwww.flickr.com/photos134486353@N07/6761047155/in/set-72157612784841131/. Accessed 1 Dec 2012.

--. 2012b. Bryozoan colony after 4 days of predation by Janolus anulatus. Available from: http://www.flickr.com/photos/34486353@N07/7217848426/in/set-72157612784841131. Accessed 1 Dec 2012.

--. and A. Hermosillo. 2008. Developmental mode in opisthobranch molluscs from the tropical Eastern Pacific Ocean. Veliger, 50:83-96.

--, M.C. Schaefer, C. Hoover, and A. Vald6s. 2013. Regional extinction of a conspicuous dorid nudibranch (Mollusca: Gastropoda) in California* Marine Biology doi:10.1007/s00227-013-2204-x.

Gosliner, T.M. 2010. Two new species of nudibranch mollusks from the coast of California. Proceed. Calif. Acad. Sci., 61:623 631.

Hadfield, M.G. and M. Switzer-Dunlap. 1984. Opisthobranchs. Pp. 209 350 in A.S. Tompa, N.H. Verdonk, and J.A.M. van den Biggelaar (eds.), The Mollusca. Vol. 7. Reproduction. Academic Press, New York.

Hamel, J.-F., P. Sargent, and A. Mercier. 2008. Diet, reproduction, settlement and growth of Palio dubia (Nudibranchia: Polyceridae) in the north-west Atlantic. J. Mar. Biol. Assoc. U.K., 88:365 374.

Hurst, A. 1967. The egg masses and veligers of thirty northeast Pacific opisthobranchs. Veliger, 9:255-288.

Ingram, W.M. 1935. Notes on egg masses associated with eight nudibranchs from Laguna Beach, California. J. Entom. Zool., Pomona College, 27:48-49.

Johnson, R.F. and T.M. Gosliner. 2012. Traditional taxonomic groupings mask evolutionary history: a molecular phylogeny and new classification of the chromodorid nudibranchs. Plos ONE, 7:e33479.

Kopp, K. 2008. July Wrinkles Dive on Catalina--Part 1 of 3 (the Macros). Available from: http://www.scubaboard.com/forums/socal/243321-july-wrinkles-dive-catalina-part-1-3-macros.html. Accessed 26 Nov 2012.

MacFarland, F.M. 1966. Studies of the Opisthobranchiate Mollusks of the Pacific coast of North America. Memoirs of the California Academy of Sciences, 6:1 546.

Marcus, E. 1961. Opisthobranch mollusks from California. Veliger, 3(Supplement): 1 85.

Marcus, Er. and Ev. Marcus. 1967. American opisthobranch mollusks. Stud. Trop. Oceanog. (Miami), 6: 1-256.

McDonald, G.R. 1983. A review of the nudibranchs of the California coast. Malacologia, 24:114-276.

--. 2007. Sacoglossa and Nudibranchia. Pp. 788-807 in J.T. Carlton (ed.), The Light and Smith manual: intertidal invertebrates from central California to Oregon. University of California Press: Berkeley, Calif. xvii+1001 pp.

Moran, A.L. and J.S. McAlister. 2009. Egg size as a life history character of marine invertebrates: is it all it's cracked up to be? Biol. Bull., 216:226-242.

Mulliner, D.K. 1972. Breeding habits and life cycles of three species of nudibranchs from the eastern Pacific. Festivus, 3:1 5.

Robilliard, G.A. 1971. A new species of Polycera (Opisthobranchia: Mollusca) from the northeastern Pacific, with notes on other species. Sysesis, 4:235-243.

Roller, R.A. 1970. A list of recommended nomenclatural changes for MacFarland's "Studies of opisthobranchiate mollusks of the Pacific coast of North America." Veliger, 12:371-374.

Steinberg, J.E. 1963. Notes on the opisthobranchs of the west coast of North America III. Further nomenclatorial changes in the order Nudibranchia. Veliger, 6:63-67.

Strathmann, M.F. 1987. Reproduction and development of marine invertebrates of the northern Pacific coast. University of Washington Press: Seattle. xii+670 pp.

Thompson, T.E. 1976. Biology of opisthobranch molluscs. Vol. I. Ray Society: London. 207 pp.

--and G.H. Brown. 1984. Biology of opisthobranch molluscs. Vol. II. Ray Society: London. 229 pp. Wolf, M. and C.M. Young. 2012. Complete development of the Northeast Pacific arminacean nudibranch Janolus fuscus. Biol. Bull., 222:137-149.

Jeffrey H.R. Goddard (1) and Brenna Green (2)

(1) Marine Science Institute, University of California, Santa Barbara, CA 93106-6150, goddard@lifesci.ucsb.edu

(2) Department of Invertebrate Zoology and Geology, California Academy of" Sciences, San Francisco, CA 94118-4503

Table 1. Collection localities.

Site                        GPS coordinates      Depth (m)/habitat

Whittier, Alaska            60.7778, -148.6903   Floating docks
Monterey Bay                36.6088, -121.8797   Subtidal, 16 m
Monterey Harbor             36.6043, -121.8912   5 m, dock pilings
Asilomar, Pacific Grove     36.6272, -121.9408   Rocky intertidal

Sand Dollar Beach,          35.9217, -121.4717   Rocky intertidal
  Monterey Co.
Cayucos                     35.4478, -120.9100   Rocky intertidal
Hazard Canyon, Montana      35.2897, -120.8839   Rocky intertidal
  de Oro State Park
Naples, Santa Barbara Co.   34.4339, -119.9514   Rocky intertidal
Naples Reef                 34.4218, -119.9523   Subtidal, 15 m
Santa Barbara Harbor        34.4067, -119.6892   Floating docks
Tarpits Reef, Carpinteria   34.3869, -119.5164   Rocky intertidal
Laguna Beach                33.5429, -117.7906   Rocky intertidal
La Jolla                    32.8549, -117.2680   subtidal, 17 m
South Casa Reef, La Jolla   32.8434, -117.2814   Rocky intertidal
Bird Rock, La Jolla         32.8144, -117.2739   Rocky intertidal
San Clemente Island         32.8134, -118.3626   subtidal, 20 m
Mission Bay, San Diego      32.7642, -117.2172   Floating docks
Point Loma, San Diego       32.6664, -117.2450   Rocky intertidal
Punta Rosarito, Baja        28.5672, -114.1597   Rocky intertidal
  California
El Tomatal, Baja            28.4869, -114.0694   Rocky intertidal
  California

Table 2. New data on embryonic development of opisthobranch
molluses from California. Mode of development: P = planktotrophic,
CM = capsular metamorphic, with modes inferred based solely on size
of zygotes or early embryos in parentheses. An asterisk (*) preceding
a species name indicates a species whose mode of development has not
previously been etermined, and a dash (-) indicates no data.
Values for egg diameter and shell length at hatching are
means [+ or -] 1 SD, and sample size (n).

                                                             Embryonic
                                Egg Diameter    # eggs per    period
  Taxon                          ([micro]m)      capsule      (days)

Sacoglossa
  Stiliger frscorittatus          58.8 [+           1           4.5
    Lance. 1962,               or -] 1.1 (10)
    CASIZ 182713

                                  60.9 [+           1           4.5
                               or -] 0.9 (10)

                                  59.6 [+           1           --
                               or -] 2.1 (9)

                                     --             l           --

Anaspidea
  Aplrsia raccaria Winkler,          --            >50          19+
    1955, CASIZ 189306

Nudipleura: Pleurobranchidae
  Berthella strongi               89.3 [+           1           16
    (MacFarland, 1966),        or -] 0.8 (10)
    CASIZ 182722
                                     --             1           --

Nudipleura: Doridina
  * Acanthodoris lutea            69.5 [+           1            8
    MacFarland, 1925           or -] 1.1 (10)

  * Anctda lentiginosa              <60            1-2          10
    Farmer in Farmer &
    Sloan, 1964,
    CASIZ 182719

  * Atagema alba                 156.6 [+           1          >>14
    (O'Donoghue, 1927),        or -] 1.7 (3)
    CASIZ 186493

  Doriopsilla albopunctata           --            1-3          --
    (Cooper, 1863)

  * Doris pickensi Marcus &       77.2 [+           1           13
    Marcus, 1967,              or -] 1.2 (10)
    CASIZ 189305

  * Felintare californiensis   [approximately       --        >7. <12
    (Bergh, 1879)                equal to]                      (c)
                                  100 (b)

  * Geitodoris tnavis Marcus      <85 (d)           1           --
    & Marcus, 1967,
    CASIZ 186482

  Okenia rosacea                     --             1           --
    (MacFarland, 1905)

  Palio dubia (M. Sars,           <75 (d)           1           --
    1829), CASIZ 182721

  Polycera tricolor               78.0 [+           1            7
    Robilliard, 1971           or -] 1.7 (10)

  Taringa aivica Marcus &         <70 (d)          4-6          --
    Marcus, 1967,
    CASIZ 186481

  * Thordisa bimaculata           79.6 [+           1            9
    Lance, 1966,               or -] 2.0 (5)
    CASIZ 182715

  Triopha maculata                70.1 [+           1           11
    MacFarland, 1905,          or -] 1.1 (10)
    CASIZ 184514

Nudipleura: Dendronotina
  Dendronotus subramosus             --             1            8
    MacFarland, 1966
    CASIZ 184512

  Doto Colombiana                 73.6 [+           l            7
    O'Donoghue, 1921,          or -] 1.9 (5)
    CASIZ 186483

  Melibe leonine                  70.4 [+          2-5           4
    (Gould, 1852).             or -] 1.6 (10)
    CASIZ 186815

Nudipleura: Arminina
  Dirona picta, MacFarland        70.1 [+          4-5           4
    in Cockerell & Eliot,      or -] 1.5 (10)
    1905, CASIZ 182714

  * Janolus anulatus              66.7 [+           1           6-7
    Camacho-Garcia &           or -] 1.4(10)
    Gosliner, 2006,
    CASIZ 189420

  Janolus barbarensis             95.4 [+         15-20          8
    (Cooper, 1863),            or -] 1.3 (10)
    CASIZ 184513

Nudipleura: Aeolidina
  * Austraeolis steamsi           81.4 [+           1            7
    (Cockerell, 1901),         or -] 2.2 (10)
    CASIZ 186486

  * Babakina festiva              72.2 [+           1           7.5
    (Roller, 1972),            or -] 2.2 (10)
    CASIZ 182204

  Cuthona lagunae                 94.9 [+           1            8
    (O'Donoghue, 1926),        or -] 1.1 (10)
    CASIZ 182716

  Cumanotus sp. CASIZ 191891        <80             1           --

  * Eubranchus sp. 2 of             <100            1            6
    Behrens & Hermosillo
    (2005), CASIZ 189422

  * Flabellina cooperi            65.4 [+          1-6           5
    (Cockerell, 1901),         or -] 2.0 (10)
    CASIZ 186489
                                     --            2-4          --

  * Flabellina goddardi           65.2 [+           1            7
    Gosliner, 2010,            or -] 0.5 (l0)
    CASIZ 182590

  Flabellina iodinea                 --            1-2          --
    (Cooper, 1862),
    CASIZ 182717
                                     --            1-2          --

  * Flabellina pricei             64.2 [+           l           --
    (MacFarland, 1966),        or -] 1.0 (10)
    CASIZ 186485

  Flabellina trilineata              --             --          --
    (O'Donoghue, 1921),
    CASIZ 182718
    CASIZ 189421
                                     --             1           7.5

                                                   l-2          --

  * Flabellina sp.                62.8 [+           1            8
    (cf F. trilineata).        or -] 2.2 (10)
    CASIZ 182720
                                     --             1            8

                                  63.8 [+           1           5.5
                               or -] 1.5 (6)

                                     --             1            5

                                     --             1            6

                                  Temp.       Shell   Shell length at
  Taxon                        ([degrees]C)   type     hatching (pm)

Sacoglossa
  Stiliger frscorittatus          15-20         1        104.3 [+
    Lance. 1962,                                      or -] 3.3 (22)
    CASIZ 182713

                                  15-20         1        108.3 [+
                                                      or -] 2.8 (22)

                                    --                      --

                                    --          1        102.7 [+
                                                      or -] 6.1 (11)

Anaspidea
  Aplrsia raccaria Winkler,         --          1     143.8 = 3.1 (5)
    1955, CASIZ 189306

Nudipleura: Pleurobranchidae
  Berthella strongi               12-16         1        137.0 [+
    (MacFarland, 1966),                               or -] 3.1 (10)
    CASIZ 182722
                                    --          1        137.1 [+
                                                       or -] 7.9 (5)

Nudipleura: Doridina
  * Acanthodoris lutea              15                   132.4 [+
    MacFarland, 1925                                  or -] 4.0 (10)

  * Anctda lentiginosa            13-18         1        99.3 [+
    Farmer in Farmer &                                or -] 3.1 (10)
    Sloan, 1964,
    CASIZ 182719

  * Atagema alba                  18-20         1       210-218 (a)
    (O'Donoghue, 1927),
    CASIZ 186493

  Doriopsilla albopunctata          --          1        193.0 [+
    (Cooper, 1863)                                     or -] 7.0 (5)

  * Doris pickensi Marcus &       14-20         1        132.6 [+
    Marcus, 1967,                                     or -] 2.2 (10)
    CASIZ 189305

  * Felintare californiensis    17-19 (c)      --           --
    (Bergh, 1879)

  * Geitodoris tnavis Marcus        --         --           --
    & Marcus, 1967,
    CASIZ 186482

  Okenia rosacea                    --          1        129.8 [+
    (MacFarland, 1905)                                or -] 1.0 (10)

  Palio dubia (M. Sars,             --
    1829), CASIZ 182721

  Polycera tricolor                 18          1        120.0 [+
    Robilliard, 1971                                  or -] 5.5 (10)

  Taringa aivica Marcus &           --         --            -
    Marcus, 1967,
    CASIZ 186481

  * Thordisa bimaculata           15-19         1        122.8 [+
    Lance, 1966,                                      or -] 6.3 (10)
    CASIZ 182715

  Triopha maculata                12-17         1        116.5 [+
    MacFarland, 1905,                                 or -] 1.8 (10)
    CASIZ 184514

Nudipleura: Dendronotina
  Dendronotus subramosus          t0-20         2        223.2 [+
    MacFarland, 1966                                  or -] 9.6 (10)
    CASIZ 184512

  Doto Colombiana                   --          1        120.1 [+
    O'Donoghue, 1921,                                 or -] 2.7 (10)
    CASIZ 186483

  Melibe leonine                    --          1        120.9 [+
    (Gould, 1852).                                    or -] 3.6 (10)
    CASIZ 186815

Nudipleura: Arminina
  Dirona picta, MacFarland        15-21         1        114.7 [+
    in Cockerell & Eliot,                             or -] 3.5 (10)
    1905, CASIZ 182714

  * Janolus anulatus              12-18         1        110.7 [+
    Camacho-Garcia &                                  or -] 2.7 (10)
    Gosliner, 2006,
    CASIZ 189420

  Janolus barbarensis             16-24         1        187.1 [+
    (Cooper, 1863),                                   or -] 5.3 (10)
    CASIZ 184513

Nudipleura: Aeolidina
  * Austraeolis steamsi             18          1        122.0 [+
    (Cockerell, 1901),                                or -] 7.3 (10)
    CASIZ 186486

  * Babakina festiva              13-17         1        115.8 [+
    (Roller, 1972),                                   or -] 1.1 (10)
    CASIZ 182204

  Cuthona lagunae                   --          2        262.1 [+
    (O'Donoghue, 1926),                                or -] 2.5 (7)
    CASIZ 182716

  Cumanotus sp. CASIZ 191891        --         --           --

  * Eubranchus sp. 2 of           12-19         2        210.4 [+
    Behrens & Hermosillo                              or -] 6.4 (10)
    (2005), CASIZ 189422

  * Flabellina cooperi              18          1        101.0 [+
    (Cockerell, 1901),                                or -] 6.9 (10)
    CASIZ 186489
                                    --          1        105.9 [+
                                                       or -] 4.2(12)

  * Flabellina goddardi           11-21         1        102.4 [+
    Gosliner, 2010,                                   or -] 2.3 (10)
    CASIZ 182590

  Flabellina iodinea                --          1        155.0 [+
    (Cooper, 1862),                                    or -] 4.3 (3)
    CASIZ 182717
                                    --          1        157.2 [+
                                                       or -] 3.2 (6)

  * Flabellina pricei               --                      --
    (MacFarland, 1966),
    CASIZ 186485

  Flabellina trilineata             --          1        103.7 [+
    (O'Donoghue, 1921),                               or -] 1.9 (10)
    CASIZ 182718
    CASIZ 189421
                                  12-19         1        105.6 [+
                                                      or -] 2.8 (10)

                                    --          1           --

  * Flabellina sp.                 9-15         1       107.6- [+
    (cf F. trilineata).                               or -] 2.0 (10)
    CASIZ 182720
                                   9-15         1        110.8 [+
                                                      or -] 2.2 (10)

                                  13-17         1        112.4 [+
                                                       or -] 3.8 (8)

                                  13-17         1        108.7 [+
                                                      or -] 1.3 (10)

                                  13-17         1        110.2 [+
                                                      or -] 1.5 (10)

                               Eyespots
                                  at        Mode of
  Taxon                        hatching   Development   Locality

Sacoglossa
  Stiliger frscorittatus          no           P        Mission Bay
    Lance. 1962,
    CASIZ 182713

                                  no                    Mission Bay

                                  --                    Monterey
                                                        Harbor

                                  no                    Monterey
                                                        Harbor

Anaspidea
  Aplrsia raccaria Winkler,       no           P        Laguna Beach
    1955, CASIZ 189306

Nudipleura: Pleurobranchidae
  Berthella strongi              yes           P        El Tomatal
    (MacFarland, 1966),
    CASIZ 182722
                                 yes                    Naples

Nudipleura: Doridina
  * Acanthodoris lutea            no           P        Monterey Bay
    MacFarland, 1925

  * Anctda lentiginosa            no           P        Naples
    Farmer in Farmer &
    Sloan, 1964,
    CASIZ 182719

  * Atagema alba                 yes          CM        Bird Rock,
    (O'Donoghue, 1927),                                 La Jolla
    CASIZ 186493

  Doriopsilla albopunctata        no           P        Cayucos
    (Cooper, 1863)

  * Doris pickensi Marcus &       no           P        Naples
    Marcus, 1967,
    CASIZ 189305

  * Felintare californiensis      --          (P)       Santa
    (Bergh, 1879)                                       Catalina
                                                        Island

  * Geitodoris tnavis Marcus      --          (P)       Punta
    & Marcus, 1967,                                     Rosarito
    CASIZ 186482

  Okenia rosacea                  no      Point Loma
    (MacFarland, 1905)

  Palio dubia (M. Sars,           -           (P)       Whittier,
    1829), CASIZ 182721                                 Alaska

  Polycera tricolor               -            P        San Clemente
    Robilliard, 1971                                    Island

  Taringa aivica Marcus &         -           (P)       Punta
    Marcus, 1967,                                       Rosarito
    CASIZ 186481

  * Thordisa bimaculata           no           P        Point Loma
    Lance, 1966,
    CASIZ 182715

  Triopha maculata                no           P        Naples
    MacFarland, 1905,
    CASIZ 184514

Nudipleura: Dendronotina
  Dendronotus subramosus          no           P        Asilomar
    MacFarland, 1966
    CASIZ 184512

  Doto Colombiana                 no           P        Hazard Canyon
    O'Donoghue, 1921,
    CASIZ 186483

  Melibe leonine                  no           P        Santa Barbara
    (Gould, 1852).                                      Harbor
    CASIZ 186815

Nudipleura: Arminina
  Dirona picta, MacFarland        no           P        Point Loma
    in Cockerell & Eliot,
    1905, CASIZ 182714

  * Janolus anulatus              no           P        Tarpits Reef
    Camacho-Garcia &
    Gosliner, 2006,
    CASIZ 189420

  Janolus barbarensis             no           P        San Diego
    (Cooper, 1863),
    CASIZ 184513

Nudipleura: Aeolidina
  * Austraeolis steamsi           no           P        La Jolla
    (Cockerell, 1901),
    CASIZ 186486

  * Babakina festiva              no           P        Naples
    (Roller, 1972),
    CASIZ 182204

  Cuthona lagunae                yes           P        Hazard Canyon
    (O'Donoghue, 1926),
    CASIZ 182716

  Cumanotus sp. CASIZ 191891      --          (P)       Santa Barbara
                                                        Harbor

  * Eubranchus sp. 2 of          yes           P        South Casa
    Behrens & Hermosillo                                Reef,
    (2005), CASIZ 189422                                La Jolla

  * Flabellina cooperi            no           P        La Jolla
    (Cockerell, 1901),
    CASIZ 186489
                                  no                    La Jolla

  * Flabellina goddardi           no           P        Tarpits Reef
    Gosliner, 2010,
    CASIZ 182590

  Flabellina iodinea              no           P        Sand Dollar
    (Cooper, 1862),                                     Beach
    CASIZ 182717
                                  no           P        Naples Reef

  * Flabellina pricei             --          (P)       La Jolla
    (MacFarland, 1966),
    CASIZ 186485

  Flabellina trilineata           no           P        Naples
    (O'Donoghue, 1921),
    CASIZ 182718
    CASIZ 189421
                                  no                    Naples

                                  --          (P)       Santa Barbara
                                                        Harbor

  * Flabellina sp.                no           P        Naples
    (cf F. trilineata).
    CASIZ 182720
                                  no                    Naples

                                  no                    Naples

                                  no                    Naples

                                  no                    Naples

(a) Shells left behind while hatching from egg capsules.

(b) Estimate based on image of egg ribbon (see Methods and Figure 2A).

(c) Hoover, personal communication to JG, 30 Sept 2012.

(d) Measurements of early embryos preserved in 70% ethanol.
COPYRIGHT 2013 Southern California Academy of Sciences
No portion of this article can be reproduced without the express written permission from the copyright holder.
Copyright 2013 Gale, Cengage Learning. All rights reserved.

Article Details
Printer friendly Cite/link Email Feedback
Author:Goddard, Jeffrey H.R.; Green, Brenna
Publication:Bulletin (Southern California Academy of Sciences)
Article Type:Report
Geographic Code:1USA
Date:Aug 1, 2013
Words:6275
Previous Article:An abnormally colored redbanded rockfish, Sebastes babcocki.
Next Article:Small mammal use of the burn perimeter following a chaparral wildfire in southern California.
Topics:

Terms of use | Privacy policy | Copyright © 2021 Farlex, Inc. | Feedback | For webmasters