Printer Friendly

Development of novel drug delivery systems using phage display technology for clinical application of protein drugs.

1. Introduction

In our post-genome era, a number of proteins thought to be involved in pathological disorders and other biological processes are considered potentially useful as therapeutic seeds or as targets for pharmaceutical development. (1-3) Therefore, comprehensive protein analyses in the healthy body and in disease conditions through disease proteomics, are now a focus of life science research and are expected to help identify proteins of therapeutic importance in various diseases. Against this background, potentially potent protein therapies that use cytokines or antibodies have recently received a great deal of attention. Indeed, attempts are currently under way to develop a wide variety of therapeutic proteins for treating conditions such as cancer, infectious diseases, and autoimmune disorders. (4-8)

Unfortunately, the clinical applications of protein drugs are still limited, except in the cases of drugs based on erythropoietin, granulocyte colony-stimulating factor, interferon-alpha, and antibodies. One difficulty lies in identifying proteins useful as therapeutic seeds or as targets for pharmaceutical development. The application of disease proteomics has enabled us to identify many candidate proteins that are differentially expressed in disease samples, but the most important issue that remains to be resolved is how to correctly identify the useful proteins from among the candidates. Another difficulty with the use of bioactive proteins, such as cytokines, as drugs is pleiotropic actions through a number of receptors in vivo, making it difficult to elicit the desired effect without simultaneously triggering undesirable secondary effects. Additionally, bioactive proteins generally have unexpectedly weak therapeutic effects. (9,10) Often these proteins are degraded by various proteases in vivo and rapidly excreted from the circulatory system. Consequently, frequent administration of an excessively high dose of a protein is required to obtain its desired therapeutic effect in vivo, leading to disturbance of homeostasis and unexpected side effects. Thus, establishment of a series of novel DDS technologies that enhance the pathway from drug exploration to optimization and overcome the abovementioned problems is essential for the advancement of protein drug development.

From this perspective, our laboratory aims to develop a novel DDS platform to overcome problems with protein therapies by: (i) establishing a high-throughput system to validate many candidate proteins by applying a phage antibody library [i.e., an in vitro monoclonal antibody (mAb) development system] to the study of disease proteomics; (11) (ii) developing a powerful system to rapidly create functional mutant proteins (muteins) with enhanced receptor affinity and receptor specificity by using a phage display technique; (12,13) and (iii) creating a novel polymer-conjugation system to dramatically improve the in vivo stability of bioactive proteins. (14) In this review, we describe these DDS technologies for advanced pharmaceutical applications.

2. Establishment of antibody proteomics technology: a high-throughput system for validation of multiple candidate proteins

Proteomics-based analysis is one of the most powerful approaches to identifying proteins useful for drug development. (1-3) The technological development of proteomics to seek and identify small amounts of proteins that are differentially expressed in diseased samples and are thus candidate therapeutic seeds or targets is expanding rapidly. However, the number of proteins successfully applied to drug development has been limited. The main difficulty is the lack of a methodology to comprehensively analyze the expression or function of many candidate proteins and to efficiently select potential proteins of interest. To circumvent this problem, we need an improved technology to efficiently screen the truly valuable proteins from among large numbers of candidates. We have therefore focused on mAbs, which are essential tools for validation of proteins. (15-17) However, the commonly used hybridoma-based mAb production requires preparation of recombinant proteins as antigens and is laborious and time-consuming, (18-21) making it impractical for creating mAbs against many candidate proteins identified by proteomics-based analysis and forcing researchers to preferentially analyze proteins of their own interest. A phage antibody library system can rapidly produce mAbs against many antigens in vitro. (22-28) We therefore applied this system to proteomics studies and developed an antibody proteomics technology that allows us to produce mAbs against many candidates to analyze their expression profiles or functions; this technology accelerates the identification of proteins potentially useful as therapeutic seeds or targets. This technology (Fig. 1) comprises (i) a search for disease-related proteins by proteomics-based analyses such as two-dimensional differential in-gel electrophoresis (2D-DIGE); (ii) identification of candidate proteins by using mass spectrometry analysis; (iii) isolation of mAbs against candidate proteins for which no commercially produced mAbs are available by using a phage antibody library; and (iv) validation of the proteins by using disease samples such as a tissue microarray, (29-33) which is a glass slide containing many clinical samples and clinical information such as age, gender, clinical stage, and treatment history. The advantages of this technology are 1) high-throughput generation of mAbs against many candidate proteins by directly using small amounts of proteins extracted from 2D-DIGE gels by optimizing the mAb selection method, and 2) validation of candidate proteins using clinical samples, even if the candidate proteins have been detected using cell lines. We can comprehensively validate each candidate protein by analyzing the correlation between its expression profile and the clinical information.

[FIGURE 1 OMITTED]

We applied this technology to various kinds of tumors and successfully identified useful proteins for drug discovery, such as cancer-specific proteins, (11,34) cancer metastasis-related proteins, (34,35) and a cisplatin resistance-related protein. (36) Here, we describe Ephrin receptor A10 (EphA10), which was identified as a novel breast cancer-related protein expressed in many breast cancer tissues but not normal breast tissues. (11) EphA10 is a relatively uncharacterized protein: the only thing known about it before our report was that it is expressed in the testis at an mRNA level. (37) Because EphA10 is a highly novel breast cancer-related protein, its production profile in normal and cancer tissues, as well as its function, need to be clarified to determine its potential as a drug target. We therefore analyzed the EphA10 production profile by using tissue microarrays and showed that EphA10 was produced in various subtypes of breast cancer--luminal A (54%), luminal B (68%), human epidermal growth factor receptor 2 (Her2)-enriched (64%), and triple negative breast cancer (TNBC) (67%)--but not in 35 kinds of normal tissue, except for the testis. (38) The TNBC subtype, which lacks the production of estrogen receptor, progesterone receptor, and Her2, is refractory to current treatments because of an absence of molecularly targeted drugs. Therefore, there is considerable interest worldwide in developing therapeutics against TNBC. (39-41) Our data suggest that EphA10 is an attractive drug target in breast cancer (particularly in patients with TNBC) and is a highly specific tumor antigen. To evaluate the utility of EphA10 as a drug target, we developed an anti-EphA10 mAb and administered it or a control mAb once a week in an intravenously xenografted mouse model. Tumor growth was significantly lower in the anti-EphA10 mAb-treated group than in the controls; this effect was dose dependent (38) (Fig. 2). EphA10 is therefore a promising drug target for treating breast cancers, including TNBC. (38,42-44)

This example shows that antibody proteomics technology is a powerful system for identifying proteins useful as therapeutic seeds or as targets for pharmaceutical development.

3. Development of a system to create artificial functional muteins for advanced pharmaceutical applications

Even though therapeutic seeds can be identified by proteome analyses such as antibody proteomics technology, the bioactive proteins generally have pleiotropic actions through a number of receptors in vivo, making it difficult to elicit the desired effect without simultaneously triggering undesirable secondary effects. It is often desirable to alter the amino acid sequence to yield artificial functional muteins with enhanced receptor affinity and receptor specificity. Traditionally, most biotechnological research facilities have used site-directed mutagenesis, for example by the classic Kunkel method, to produce functional muteins. (45,46) However, the creation of muteins by point mutations requires the generation of many individual mutants by replacing specific amino acids through a process of trial and error. The overall process of generating bioactive proteins with the desired properties is therefore time consuming and costly. Consequently, the variety of functional muteins is often limited, making it difficult to achieve the desired enhancement of therapeutic effect.

[FIGURE 2 OMITTED]

To solve these problems, phage display systems, including the phage antibody library described above, have recently been developed for constructing libraries of muteins displayed on a bacteriophage surface; these systems facilitate rapid screening against a given target (47-49) (Fig. 3). From such libraries, the desired phages (e.g. phages displaying muteins with high affinity to target proteins) can be selected, isolated, and then expanded by application of a panning procedure. Moreover, the relevant gene sequence is readily determined because the selected phage contains the gene that encodes the desired protein. The range of applications of the phage display method as a standard technology for quick and efficient screening of molecules that bind to particular targets is constantly increasing.

From this perspective, we have been trying to create functional muteins for advanced pharmaceutical applications, using tumor necrosis factor-alpha (TNF) as an example. We previously constructed a phage library displaying mutated TNFs with substitutions of amino acids at the receptor binding sites predicted on the basis of a 3D structure of TNF receptor 1 (TNFR1) or 2 (TNFR2). (50,51) We then successfully created various kinds of functional TNF muteins, such as those with enhanced affinity to TNFR1 or TNFR2, those with altered binding selectivity for the receptors, (12,13,52) and lysine deficient muteins with full bioactivity. (14) Here, we describe the TNFR1-selective antagonistic mutant TNF (R1antTNF), which is the first mutein modified from agonist to antagonist (12,13) (Fig. 4). Nowadays, TNF inhibitors such as anti-TNF mAbs have been used as effective drugs against autoimmune conditions, including rheumatoid arthritis. (53-55) However, the drugs have serious side effects such as infections or increased cancer risk, (56,57) because they inhibit functions via both TNFR1 (induction of inflammation) (58,59) and TNFR2 (protection against infection). (60,61) Therefore, an agent that inhibits only TNFR1-mediated function is urgently needed. In this respect, R1antTNF can be useful because it displays TNFR1-selective binding and therefore selective inhibition of TNFR1-mediated biological activity in vitro (Fig. 4). R1antTNF may thus be an attractive therapeutic mutein. In fact, the therapeutic effects of R1antTNF in models of acute, potentially lethal hepatitis are as good as, or better than, those obtained by using conventional anti-TNF mAb therapy. (62) By using these technologies, we have also been able to create many functional muteins for various proteins such as interferons, lymphotoxinalpha, and LIGHT. (63,64) This technology is versatile and can be used routinely to create functional muteins.

[FIGURE 3 OMITTED]

4. A new approach to site-specific PEGylation: creation of a polymer-conjugation system to improve the in vivo stability of bioactive proteins

We expect that RlantTNF will also have therapeutic effects in models of chronic inflammatory diseases, such as a model of collagen-induced arthritis and a model of experimental autoimmune encephalomyelitis. However, both RlantTNF and wild-type TNF (wtTNF) have short half-lives (about 10min) in the plasma of mice. A method of extending the half-life of RlantTNF in the plasma is therefore needed.

Conjugation of water-soluble polymers such as polyethylene glycol (PEG) to bioactive proteins dramatically improves protein stability in vivo (65-67) (Fig. 5). Bioconjugation of PEG to bioactive proteins --a process known as PEGylation--increases the molecular weight of the protein and decreases the rate of renal clearance. Additionally, because water-soluble polymers cover the protein surface, attack by proteases is generally blocked through steric hindrance, resulting in prolongation of the half-life in vivo. Moreover, steric hindrance decreases antigenicity and immunogenicity. Because of these advantages, it is possible to use the bioactive protein at a lower dose. Some bioactive proteins such as granulocyte-colony stimulating factor, interferon-alpha, asparaginase, and adenosine deaminase have been successfully PEGylated, and this process gives them greater therapeutic efficacy than their corresponding native forms. (68-72) These findings suggest that PEGylation will become an important technique for expanding the clinical application of therapeutic proteins. However, in reality, the successful application of PEGylation is limited, because the polymers react with or mask the active site of the bioactive protein. PEGylation generally targets not only the N-terminal [alpha]-amino group but also the [epsilon]-amino group of lysine residues, which are often important for the formation of multi-dimensional structures and in the binding between ligands and receptors. Therefore, introducing polymers at these sites can potentially reduce the biological activity of the protein. (73,74) Indeed, PEGylated interferon, the use of which has raised hopes as a potential cure for hepatitis C, can be produced only as a heterogeneous mixture with only 10% to 30% of its anticipated activity. (74) Improvement in bioconjugation efficiency will require the creation of a system that maintains the current efficiency of polymer modification and simultaneously makes it possible to modify only specific sites within a protein.

We have developed a novel PEGylation system combined with a method of creating functional muteins. In our strategy, site-specific PEGylation to improve therapeutic potency is established by using a fully bioactive lysine-deficient mutant. (14) Whereas conventional PEGylation of TNF causes a loss of bioactivity due to random introduction of PEG at the [epsilon]-amino groups of six lysine residues in monomeric TNF, our site-specific PEGylation introduces PEG only at the N-terminus via a lysine-deficient mutant TNF, without loss of bioactivity. R1antTNF was generated by using a phage library based on the lysine-deficient mutant of TNF. Consequently, the R1antTNF also lacked lysine residues. (12,13) Interestingly, the N-terminus of TNF is dispensable for function, because a deletion mutant of TNF lacking eight residues at the N-terminus retains full bioactivity. (75) Thus, site-specific PEGylated R1antTNF (PEG-R1antTNF) was uniform at the molecular level and had similar bioactivity (80%) to that of unmodified R1antTNF. Furthermore, introducing PEG only to the N-terminal amino group made it possible to produce stable bioconjugated proteins with nearly 100% yield. In fact, PEG-R1antTNF had therapeutic effects in an experimental autoimmune encephalomyelitis model (76) and a collagen-induced arthritis model (77) (Fig. 6). This could have been due to the enhanced retention of R1antTNF in the circulatory system. Moreover, the prolonged retention might increase the availability of R1antTNF to block TNF/TNFR1 interactions in the general circulation or in the lesion areas, resulting in improved inhibitory activity. In future, we hope that PEG-R1antTNF will be a therapeutic option for managing chronic inflammatory diseases and that our site-specific PEGylation system will become a standard technology for clinical application of protein drugs.

[FIGURE 6 OMITTED]

5. Conclusions and prospects

Despite the enhancement of basic research in Japan, clinical application of protein drugs remains limited because of the poor nature of fundamental platforms for drug discovery. Here, we discussed a series of DDS technologies needed for protein drug development. Moreover, we have recently succeeded in developing functionalized polymer carriers (78) or nano-carriers, (79,80) which are able to regulate drug delivery into various tissues such as the placenta or brain, and into cellular organelles such as nuclei. Furthermore, we consider not only efficacy but also safety to be important in drug development. We have thus been promoting the fusion of sustainable nanotechnology with nano-safety science for assuring the safety of nanomaterials and nano-safety design study for enhancing safety of nanomaterials. (81-83) We hope that many innovative protein drugs will be developed in Japan by combining these technologies.

doi: 10.2183/pjab.92.156

Acknowledgments

We thank our staff and our collaborators in this long-term project, especially S. Nagata, T. Ise, S. Tsunoda, H. Kamada, Y. Mukai, and T. Mori (National Institutes of Biomedical Innovation, Health, and Nutrition); Y. Yoshioka, H. Takahashi, and K. Misato (Research Institute for Microbial Diseases, Osaka University); H. Shibata, H. Nabeshi, T. Toshida, and Y. Abe (National Institute of Health Sciences); and T. Yoshikawa and K. Higashisaka (Graduate School of Pharmaceutical Sciences, Osaka university) for supporting this study and for their fruitful discussions. We also thank the staff and students of the Laboratory of Biotechnology and Therapeutics, Laboratory of Toxicology and Safety Science (Osaka University), Laboratory of Biopharmaceutical Research, Laboratory of innovative Antibody Engineering and Design, and Laboratory of Pharmaceutical Proteomics (National Institutes of Biomedical Innovation, Health, and Nutrition). Finally, we sincerely thank our former teachers, T. Mayumi (Emeritus Professor, Osaka University), T. Kawanishi (Director, National Institute of Health Sciences), K. Yamanishi (Director, The Research Foundation for Microbial Diseases, Osaka University), Y. Yoneda (Director, National Institutes of Biomedical Innovation, Health, and Nutrition).

This study was financially supported in part by Grants-in-Aid for Scientific Research from the Ministry of Education, Culture, Sports, Science, and Technology of Japan and from the Japan Society for the Promotion of Science. This study was also supported in part by Health Labour Sciences Research Grants from the Ministry of Health, Labour, and Welfare of Japan; by Health Sciences Research Grants for Research on Publicly Essential Drugs and Medical Devices from the Japan Health Sciences Foundation; by a Global Environment Research Fund from the Ministry of the Environment; and by the Knowledge Cluster Initiative, The Nagai Foundation Tokyo, and the Takeda Science Foundation.

References

(1) Hanash, S. (2003) Disease proteomics. Nature 422, 226-232. 17)

(2) Kavallaris, M. and Marshall, G.M. (2005) Proteo mics and disease: opportunities and challenges. Med. J. Aust. 182, 575-579.

(3) Oh-Ishi, M. and Maeda, T. (2007) Disease proteomics of high-molecular-mass proteins by two-dimensional gel electrophoresis with agarose gels in the first dimension (Agarose 2-DE). J. Chromatogr. B Analyt. Technol. Biomed. Life Sci. 849, 211-222.

(4) Goldenberg, M.M. (1999) Trastuzumab, a recombinant DNA-derived humanized monoclonal anti-body, a novel agent for the treatment of metastatic breast cancer. Clin. Ther. 21, 309-318.

(5) Leget, G.A. and Czuczman, M.S. (1998) Use of rituximab, the new FDA-approved antibody. Curr. Opin. Oncol. 10, 548-551.

(6) Tamizhmani, T., Mukherjee, P.K., Manimaran, S., Subburaj, T. and Suresh, B. (2003) Some less known central nervous system depressant. Anc. Sci. Life 23, 35-39.

(7) Weinblatt, M.E., Kremer, J.M., Bankhurst, A.D., Bulpitt, K.J., Fleischmann, R.M., Fox, R.I., Jackson, C.G., Lange, M. and Burge, D.J. (1999) A trial of etanercept, a recombinant tumor necrosis factor receptor:Fc fusion protein, in patients with rheumatoid arthritis receiving methotrexate. N. Engl. J. Med. 340, 253-259.

(8) Henry, D.H., Bowers, P., Romano, M.T. and Provenzano, R. (2004) Epoetin alfa. Clinical evolution of a pleiotropic cytokine. Arch. Intern. Med. 164, 262-276.

(9) Kimura, K. et al. (1987) Phase I study of recombinant human tumor necrosis factor. Cancer Chemother. Pharmacol. 20, 223-229.

(10) Rosenberg, S.A. et al. (1987) A progress report on the treatment of 157 patients with advanced cancer using lymphokine-activated killer cells and interleukin-2 or high-dose interleukin-2 alone. N. Engl. J. Med. 316, 889-897.

(11) Imai, S. et al. (2011) Development of an antibody proteomics system using a phage antibody library for efficient screening of biomarker proteins. Bio-materials 32, 162-169.

(12) Shibata, H. et al. (2008) Creation and X-ray structure analysis of the tumor necrosis factor receptor-1selective mutant of a tumor necrosis factor-alpha antagonist. J. Biol. Chem. 283, 998-1007.

(13) Mukai, Y. et al. (2009) Structure-function relation ship of tumor necrosis factor (TNF) and its 28) receptor interaction based on 3D structural analysis of a fully active TNFR1-selective TNF mutant. J. Mol. Biol. 385, 1221-1229.

(14) Yamamoto, Y. et al. (2003) Site-specific PEGylation of a lysine-deficient TNF-alpha with full bioactivity. Nat. Biotechnol. 21, 546-552.

(15) Chaga, G.S. (2008) Antibody arrays for determination of relative protein abundances. Methods Mol. Biol. 441, 129-151. 30)

(16) Kaufmann, H., Bailey, J.E. and Fussenegger, M. (2001) Use of antibodies for detection of phosphorylated proteins separated by two-dimensional gel electrophoresis. Proteomics 1, 194-199.

(17) Xu, Z.W., Zhang, T., Song, C.J., Li, Q., Zhuang, R., Yang, K., Yang, A.G. and Jin, B.Q. (2008) Application of sandwich ELISA for detecting tag fusion proteins in high throughput. Appl. Microbiol. Biotechnol. 81, 183-189.

(18) Asadi, A., Pourfathollah, A.A., Mahdavi, M., Eftekharian, M.M. and Moazzeni, S.M. (2008) Preparation of antibody against horseradish peroxidase using hybridoma technology. Hum. Antibodies 17, 73-78.

(19) Hadas, E. and Theilen, G. (1987) Production of monoclonal antibodies. The effect of hybridoma concentration on the yield of antibody-producing clones. J. Immunol. Methods 96, 3-6.

(20) Makonkawkeyoon, L., Pharephan, S. and Makonkawkeyoon, S. (2006) Production of a mouse hybridoma secreting monoclonal antibody highly specific to hemoglobin Bart's (gamma4). Lab. Hematol. 12, 193-200.

(21) McKinney, K.L., Dilwith, R. and Belfort, G. (1995) Optimizing antibody production in batch hybridoma cell culture. J. Biotechnol. 40, 31-48.

(22) Barbas, C.F. 3rd, Kang, A.S., Lerner, R.A. and Benkovic, S.J. (1991) Assembly of combinatorial antibody libraries on phage surfaces: the gene III site. Proc. Natl. Acad. Sci. U.S.A. 88, 7978-7982.

(23) Coomber, D.W. (2002) Panning of antibody phage-display libraries. Standard protocols. Methods Mol. Biol. 178, 133-145.

(24) McCafferty, J., Griffiths, A.D., Winter, G. and Chiswell, D.J. (1990) Phage antibodies: filamentous phage displaying antibody variable domains. Nature 348, 552-554.

(25) Okamoto, T. et al. (2004) Optimal construction of non-immune scFv phage display libraries from mouse bone marrow and spleen established to select specific scFvs efficiently binding to antigen. Biochem. Biophys. Res. Commun. 323, 583-591.

(26) Smith, G.P. (1985) Filamentous fusion phage: novel expression vectors that display cloned antigens on the virion surface. Science 228, 1315-1317.

(27) Vaughan, T.J., Williams, A.J., Pritchard, K., Osbourn, J.K., Pope, A.R., Earnshaw, J.C., McCafferty, J., Hodits, R.A., Wilton, J. and Johnson, K.S. (1996) Human antibodies with subnanomolar affinities isolated from a large nonimmunized phage display library. Nat. Biotechnol. 14, 309-314.

(28) Imai, S. et al. (2006) Quality enhancement of the non-immune phage scFv library to isolate effective antibodies. Biol. Pharm. Bull. 29, 1325-1330.

(29) Au, N.H., Gown, A.M., Cheang, M., Huntsman, D., Yorida, E., Elliott, W.M., Flint, J., English, J., Gilks, C.B. and Grimes, H.L. (2004) P63 expression in lung carcinoma: a tissue microarray study of 408 cases. Appl. Immunohistochem. Mol. Morphol. 12, 240-247.

(30) de Jong, D. et al. (2009) Immunohistochemical prognostic markers in diffuse large B-cell lymphoma: validation of tissue microarray as a prerequisite for broad clinical applications (a study from the Lunenburg Lymphoma Biomarker Consortium). J. Clin. Pathol. 62, 128-138.

(31) Kozarova, A., Petrinac, S., Ali, A. and Hudson, J.W. (2006) Array of informatics: Applications in modern research. J. Proteome Res. 5, 1051-1059.

(32) Rimm, D.L., Camp, R.L., Charette, L.A., Costa, J., Olsen, D.A. and Reiss, M. (2001) Tissue microarray: a new technology for amplification of tissue resources. Cancer J. 7, 24-31.

(33) Tawfik El-Mansi, M. and Williams, A.R. (2006) Validation of tissue microarray technology using cervical adenocarcinoma and its precursors as a model system. Int. J. Gynecol. Cancer 16, 1225-1233.

(34) Nagano, K. et al. (2015) Identification and evaluation of metastasis-related proteins, oxysterol binding protein-like 5 and calumenin, in lung tumors. Int. J. Oncol. 47, 195-203.

(35) Yamashita, T. et al. (2012) Rho GDP-dissociation inhibitor alpha is associated with cancer metastasis in colon and prostate cancer. Pharmazie 67, 253-255.

(36) Yamashita, T. et al. (2012) Annexin A4 is a possible biomarker for cisplatin susceptibility of malignant mesothelioma cells. Biochem. Biophys. Res. Commun. 421, 140-144.

(37) Aasheim, H.C., Patzke, S., Hjorthaug, H.S. and Finne, E.F. (2005) Characterization of a novel Eph receptor tyrosine kinase, EphA10, expressed in testis. Biochim. Biophys. Acta 1723, 1-7.

(38) Nagano, K. et al. (2014) Ephrin receptor A10 is a promising drug target potentially useful for breast cancers including triple negative breast cancers. J. Control. Release 189, 72-79.

(39) Carey, L., Winer, E., Viale, G., Cameron, D. and Gianni, L. (2010) Triple-negative breast cancer: disease entity or title of convenience? Nat. Rev. Clin. Oncol. 7, 683-692.

(40) Pal, S.K., Childs, B.H. and Pegram, M. (2011) Triple negative breast cancer: unmet medical needs. Breast Cancer Res. Treat. 125, 627-636.

(41) Podo, F. et al. (2010) Triple-negative breast cancer: present challenges and new perspectives. Mol. Oncol. 4, 209-229.

(42) Nagano, K. et al. (2013) Expression of Eph receptor A10 is correlated with lymph node metastasis and stage progression in breast cancer patients. Cancer Med. 2, 972-977.

(43) Nagano, K., Yamashita, T., Inoue, M., Higashisaka, K., Yoshioka, Y., Abe, Y., Mukai, Y., Kamada, H., Tsutsumi, Y. and Tsunoda, S. (2014) Eph receptor A10 has a potential as a target for a prostate cancer therapy. Biochem. Biophys. Res. Commun. 450, 545-549.

(44) Kamada, H., Taki, S., Nagano, K., Inoue, M., Ando, D., Mukai, Y., Higashisaka, K., Yoshioka, Y., Tsutsumi, Y. and Tsunoda, S. (2015) Generation and characterization of a bispecific diabody targeting both EPH receptor A10 and CD3. Biochem. Biophys. Res. Commun. 456, 908-912.

(45) Tsutsumi, Y., Onda, M., Nagata, S., Lee, B., Kreitman, R.J. and Pastan, I. (2000) Site-specific chemical modification with polyethylene glycol of recombinant immunotoxin anti-Tac(Fv)-PE38 (LMB-2) improves antitumor activity and reduces animal toxicity and immunogenicity. Proc. Natl. Acad. Sci. U.S.A. 97, 8548-8553.

(46) Onda, M., Nagata, S., Tsutsumi, Y., Vincent, J.J., Wang, Q., Kreitman, R.J., Lee, B. and Pastan, I. (2001) Lowering the isoelectric point of the Fv portion of recombinant immunotoxins leads to decreased nonspecific animal toxicity without affecting antitumor activity. Cancer Res. 61, 5070-5077.

(47) Chowdhury, P.S. and Pastan, I. (1999) Improving antibody affinity by mimicking somatic hypermutation in vitro. Nat. Biotechnol. 17, 568-572.

(48) Hoogenboom, H.R. and Chames, P. (2000) Natural and designer binding sites made by phage display technology. Immunol. Today 21, 371-378.

(49) Sblattero, D. and Bradbury, A. (2000) Exploiting recombination in single bacteria to make large phage antibody libraries. Nat. Biotechnol. 18, 75-80.

(50) Mukai, Y., Nakamura, T., Yoshioka, Y., Tsunoda, S., Kamada, H., Nakagawa, S., Yamagata, Y. and Tsutsumi, Y. (2009) Crystallization and preliminary X-ray analysis of the tumour necrosis factor alpha-tumour necrosis factor receptor type 2 complex. Acta Crystallogr. Sect. F Struct. Biol. Cryst. Commun. 65, 295-298.

(51) Mukai, Y., Nakamura, T., Yoshikawa, M., Yoshioka, Y., Tsunoda, S., Nakagawa, S., Yamagata, Y. and Tsutsumi, Y. (2010) Solution of the structure of the TNF-TNFR2 complex. Sci. Signal. 3, ra83.

(52) Shibata, H. et al. (2010) Generation of mouse macrophages expressing membrane-bound TNF variants with selectivity for TNFR1 or TNFR2. Cytokine 50, 75-83.

(53) Feldmann, M. and Maini, R.N. (2003) Lasker Clinical Medical Research Award. TNF defined as a therapeutic target for rheumatoid arthritis and other autoimmune diseases. Nat. Med. 9, 1245-1250.

(54) Thorbecke, G.J., Shah, R., Leu, C.H., Kuruvilla, A.P., Hardison, A.M. and Palladino, M.A. (1992) Involvement of endogenous tumor necrosis factor alpha and transforming growth factor beta during induction of collagen type II arthritis in mice. Proc. Natl. Acad. Sci. U.S.A. 89, 7375-7379.

(55) Williams, R.O., Feldmann, M. and Maini, R.N. (1992) Anti-tumor necrosis factor ameliorates joint disease in murine collagen-induced arthritis. Proc. Natl. Acad. Sci. U.S.A. 89, 9784-9788.

(56) Gomez-Reino, J.J., Carmona, L., Valverde, V.R., Mola, E.M., Montero, M.D. and Group, B. (2003) Treatment of rheumatoid arthritis with tumor necrosis factor inhibitors may predispose to significant increase in tuberculosis risk: a multicenter active-surveillance report. Arthritis Rheum. 48, 2122-2127.

(57) Lubel, J.S., Testro, A.G. and Angus, P.W. (2007) Hepatitis B virus reactivation following immunosuppressive therapy: guidelines for prevention and management. Intern. Med. J. 37, 705-712.

(58) Mori, L., Iselin, S., De Libero, G. and Lesslauer, W. (1996) Attenuation of collagen-induced arthritis in 55-kDa TNF receptor type 1 (TNFR1)-IgG1-treated and TNFR1-deficient mice. J. Immunol. 157, 3178-3182.

(59) Keffer, J., Probert, L., Cazlaris, H., Georgopoulos, S., Kaslaris, E., Kioussis, D. and Kollias, G. (1991) Transgenic mice expressing human tumour necrosis factor: a predictive genetic model of arthritis. EMBO J. 10, 4025-4031.

(60) Olleros, M.L., Guler, R., Corazza, N., Vesin, D., Eugster, H.P., Marchal, G., Chavarot, P., Mueller, C. and Garcia, I. (2002) Transmembrane TNF induces an efficient cell-mediated immunity and resistance to Mycobacterium bovis bacillus Calmette-Guerin infection in the absence of secreted TNF and lymphotoxin-alpha. J. Immunol. 168, 3394-3401.

(61) Saunders, B.M., Tran, S., Ruuls, S., Sedgwick, J.D., Briscoe, H. and Britton, W.J. (2005) Transmembrane TNF is sufficient to initiate cell migration and granuloma formation and provide acute, but not long-term, control of Mycobacterium tuberculosis infection. J. Immunol. 174, 4852-4859.

(62) Shibata, H. et al. (2008) The therapeutic effect of TNFR1-selective antagonistic mutant TNF-alpha in murine hepatitis models. Cytokine 44, 229-233.

(63) Yoshioka, Y. et al. (2010) Creation of lysine-deficient mutant lymphotoxin-alpha with receptor selectivity by using a phage display system. Biomaterials 31, 1935-1943.

(64) Morishige, T., Yoshioka, Y., Inakura, H., Tanabe, A., Yao, X., Tsunoda, S., Tsutsumi, Y., Mukai, Y., Okada, N. and Nakagawa, S. (2010) Creation of a LIGHT mutant with the capacity to evade the decoy receptor for cancer therapy. Biomaterials 31, 3357-3363.

(65) Aghemo, A., Rumi, M.G. and Colombo, M. (2010) Pegylated interferons alpha2a and alpha2b in the treatment of chronic hepatitis C. Nat. Rev. Gastroenterol. Hepatol. 7, 485-494.

(66) Yoshioka, Y., Tsutsumi, Y., Nakagawa, S. and Mayumi, T. (2004) Recent progress on tumor missile therapy and tumor vascular targeting therapy as a new approach. Curr. Vasc. Pharmacol. 2, 259-270.

(67) Pasut, G. and Veronese, F.M. (2009) PEG conjugates in clinical development or use as anticancer agents: an overview. Adv. Drug Deliv. Rev. 61, 1177-1188.

(68) Hershfield, M.S. (1995) PEG-ADA replacement therapy for adenosine deaminase deficiency: an update after 8.5 years. Clin. Immunol. Immunopathol. 76, S228-S232.

(69) Chapes, S.K., Simske, S.J., Sonnenfeld, G., Miller, E.S. and Zimmerman, R.J. (1999) Effects of spaceflight and PEG-IL-2 on rat physiological and immunological responses. J. Appl. Physiol. (1985) 86, 2065-2076.

(70) Maeda, H. (2001) SMANCS and polymer-conjugated macromolecular drugs: advantages in cancer chemotherapy. Adv. Drug Deliv. Rev. 46, 169-185.

(71) Talpaz, M., O'Brien, S., Rose, E., Gupta, S., Shan, J., Cortes, J., Giles, F.J., Faderl, S. and Kantarjian, H.M. (2001) Phase 1 study of polyethylene glycol formulation of interferon alpha-2B (Schering 54031) in Philadelphia chromosomepositive chronic myelogenous leukemia. Blood 98, 1708-1713.

(72) Isidori, A. et al. (2005) Phase II study of a single pegfilgrastim injection as an adjunct to chemotherapy to mobilize stem cells into the peripheral blood of pretreated lymphoma patients. Haematologica 90, 225-231.

(73) Monkarsh, S.P. et al. (1997) Positional isomers of monopegylated interferon alpha-2a: isolation, characterization, and biological activity. Anal. Biochem. 247, 434-440.

(74) Bailon, P. et al. (2001) Rational design of a potent, long-lasting form of interferon: a 40kDa branched polyethylene glycol-conjugated interferon alpha-2a for the treatment of hepatitis C. Bioconjug. Chem. 12, 195-202.

(75) Goh, C.R. and Porter, A.G. (1991) Structural and functional domains in human tumour necrosis factors. Protein Eng. 4, 385-389.

(76) Nomura, T. et al. (2011) Therapeutic effect of PEGylated TNFR1-selective antagonistic mutant TNF in experimental autoimmune encephalomyelitis mice. J. Control. Release 149, 8-14.

(77) Shibata, H. et al. (2009) The treatment of established murine collagen-induced arthritis with a TNFR1-selective antagonistic mutant TNF. Biomaterials 30, 6638-6647.

(78) Kamada, H., Tsutsumi, Y., Sato-Kamada, K., Yamamoto, Y., Yoshioka, Y., Okamoto, T., Nakagawa, S., Nagata, S. and Mayumi, T. (2003) Synthesis of a poly(vinylpyrrolidone-co-dimethyl maleic anhydride) co-polymer and its application for renal drug targeting. Nat. Biotechnol. 21, 399-404.

(79) Nabeshi, H. et al. (2011) Systemic distribution, nuclear entry and cytotoxicity of amorphous nanosilica following topical application. Biomaterials 32, 2713-2724.

(80) Yamashita, K. et al. (2011) Silica and titanium dioxide nanoparticles cause pregnancy complications in mice. Nat. Nanotechnol. 6, 321-328.

(81) Nabeshi, H. et al. (2011) Amorphous nanosilica induce endocytosis-dependent ROS generation and DNA damage in human keratinocytes. Part. Fibre Toxicol. 8, 1.

(82) Hirai, T. et al. (2012) Amorphous silica nanoparticles size-dependently aggravate atopic dermatitis-like skin lesions following an intradermal injection. Part. Fibre Toxicol. 9, 3.

(83) Yoshida, T. et al. (2013) Intranasal exposure to amorphous nanosilica particles could activate intrinsic coagulation cascade and platelets in mice. Part. Fibre Toxicol. 10, 41.

(Received Feb. 1, 2016; accepted Mar. 29, 2016)

By Kazuya NAGANO * [1], * [2] and Yasuo TSUTSUMI * [1], * [2], * [3], ([dagger]) (Communicated by Shigekazu NAGATA, M.J.A.)

* [1] Laboratory of Toxicology and Safety Science, Graduate School of Pharmaceutical Sciences, Osaka University, Suita, Osaka, Japan.

* [2] Laboratory of innovative Antibody Engineering and Design, Center for Drug Design Research, National Institute of Biomedical Innovation, Ibaraki, Osaka, Japan.

* [3] The Center for Advanced Medical Engineering and Informatics, Osaka University, Suita, Osaka, Japan.

([dagger]) Correspondence should be addressed: Y. Tsutsumi, Laboratory of Toxicology and Safety Science, Graduate School of Pharmaceutical Sciences, Osaka University, 1-6 Yamadaoka, Suita, Osaka 565-0871, Japan (e-mail: ytsutsumi@phs.osaka-u. ac.jp).

Profile

Kazuya Nagano was born in Hokkaido in 1980. He graduated from School of Pharmaceutical Sciences, University of Shizuoka in 2005 and completed a master's degree of Graduate School of Pharmaceutical Sciences, Osaka University in 2007. In 2007, he started his research carrier as a research fellowship for young scientist in Japan Society for the Promotion of Science (DC1<doctoral course 1>). And then he was appointed as a research fellow in Laboratory of Pharmaceutical Proteomics Project, National Institute of Biomedical Innovation (Project Leader Yasuo Tsutsumi) from 2009 to 2010 and in Laboratory of Biopharmaceutical Research, National Institute of Biomedical Innovation (Project Leader Shin-ichi Tsunoda) from 2010 to 2015. He was also appointed as visiting researcher in Graduate School of Pharmaceutical Sciences, Osaka University from 2013 to 2015 and received Ph.D. degree from Osaka University in 2014. Currently, he is in position of an associate professor in Laboratory of Toxicology and Safety Science, Graduate School of Pharmaceutical Sciences, Osaka University (Professor Yasuo Tsutsumi) and a visiting researcher in Laboratory of Innovative Antibody Engineering and Design, National Institutes of Biomedical Innovation, Health and Nutrition (Invited Project Leader Yasuo Tsutsumi). Throughout his carrier, he has searched for drug targets or drug seeds and tried to develop antibody drugs. He mainly contributes to development of antibody proteomics system. From these research outcomes, he has received various awards from University of Shizuoka (2005), Kinki Branch of the Pharmaceutical Society of Japan (2009), Japanese Association for Metastasis Research (2010), Japan Society of Drug Delivery System (2012) and Clinical Pharmaceutical Sciences, the Pharmaceutical Society of Japan (2014).

Profile

Yasuo Tsutsumi was born in Osaka in 1969. He graduated from School of Pharmaceutical Sciences, Osaka University in 1991 and completed a master's degree of the graduate school in 1993. In 1994, he started his research carrier as an assistant professor in Department of Biopharmaceutics, the same school (Prof. Tadanori Mayumi) and received Ph.D. degree from Osaka University in 1997. He experienced a project head in Disease-Proteomics Project, National Institute of Health Science from 2004 to 2005 and then became a project leader in Laboratory of Pharmaceutical Proteomics, National Institute of Biomedical Innovation from 2005 to 2010. In 2008, he has been appointed as a professor in Laboratory of Toxicology and Safety Science, Graduate School of Pharmaceutical Sciences, Osaka University and in The Center for Advanced Medical Engineering and Informatics, Osaka University. Currently, he is also in position of a dean in Graduate School of Pharmaceutical Sciences, Osaka University from 2012 and an invited project leader in Laboratory of Innovative Antibody Engineering and Design, National Institutes of Biomedical Innovation, Health and Nutrition. Throughout his carrier, he has constructed fundamental technologies for protein drug development and discovered novel protein drug candidates including R1antiTNF. Recently, he has assessed efficacy and toxicity of nanomaterials and designed effective and safe nano-carriers. From these research outcomes, he has received various awards from Academy of Pharmaceutical Science and Technology, Japan (2004), the Pharmaceutical Society of Japan (2004), Japan Society of Drug Delivery System (2009) and Japan Society for the Promotion of Science (2014).
Fig. 4. Antagonistic activities of the tumor necrosis factor
receptor 1-selective mutein (R1antTNF). a) Dissociation constants
for the interaction between TNFR1 and wild-type TNF
(wtTNF) or R1antTNF were calculated by surface plasmon
resonance analysis. Selectivity of R1antTNF for TNFR1 was
normalized to that of wtTNF. b) LM cells, mouse fibroblast cells,
were incubated with serial dilutions of R1antTNF only (open
circles) or R1ant TNF mixed with human wild-type TNF
(wtTNF) (closed circles). The inhibitory effects of R1antTNF
on the cytotoxicity of wtTNF were assessed by a methylene blue
assay. The absorbance of cells without wtTNF was plotted as
100% viability.

a) TNFR1 selectivity of RlantTNF

Clone     TNFR1 Kd   TNFR2 Kd   Selectivity
             (nM)        (nM)     for TNFR1

wtTNF         1.4         2.1           1.0
R1antTNF      3.5     92900.0       17677.4

Fig. 5. Advantages of PEGylation. Chemical modification of
bioactive proteins with polyethylene glycol (PEG) is known as
PEGylation. PEGylation prolongs the half-lives of proteins and
allows them to be used at lower doses.

Advantages of PEGylation

1. Blocks protease attack

2. Decreases renal excretion rate by increasing molecular size

3. Reduces immunogenicity
COPYRIGHT 2016 The Japan Academy
No portion of this article can be reproduced without the express written permission from the copyright holder.
Copyright 2016 Gale, Cengage Learning. All rights reserved.

 
Article Details
Printer friendly Cite/link Email Feedback
Author:Nagano, Kazuya; Tsutsumi, Yasuo
Publication:Japan Academy Proceedings Series B: Physical and Biological Sciences
Article Type:Report
Date:May 1, 2016
Words:6096
Previous Article:X-ray studies of neutron stars and their magnetic fields.
Next Article:Potential uses of stable isotope ratios of Sr, Nd, and Pb in geological materials for environmental studies.
Topics:

Terms of use | Privacy policy | Copyright © 2018 Farlex, Inc. | Feedback | For webmasters