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Determining and profiling the prevelence of antibiotics--resistant bacteria (ARBs) in the waters of the Ballona watershed.

82 DETERMINING AND PROFILING THE PREVELENCE OF ANTIBIOTICS -RESISTANT BACTERIA (ARBs) IN THE WATERS OF THE BALLONA WATERSHED

The overuse of antibiotics has led to the rise of antibiotics-resistant microbes in the environment. Recently, a prevalence of bacterial strains with insensitivities some types of antibiotics have been found in the Ballona Wetlands. This investigation addressed the Ballona Wetland's effectiveness in reducing these bacterial counts. Ebb/flood tide, and sediment samples were collected and the bacteria was extracted from the samples and plated. A standard replication method was then used to replicate colony growth and the tryptic soy agar plates were then infused with antibiotics. Photos of replica plates were taken and were analyzed and colonies were identified. Then, the bacteria were tested to determine the presence of ABIs using the Kirby-Bauer method. Cumulative frequencies of ABIs of flood, ebb, and sediment samples were compared using the Kolmogorov-Smirnov test. If the wetlands were functioning properly, bacterial counts would be reduced during ebb tide and that there would be a significant difference between the ebb and flow tide samples, however they were nearly identical. It was also expected that the cumulative frequency of ABIs found in sediment would have a very different trend, yet the trends were very similar to ebb and flood. The existence of bacteria resistant to a wide spectrum of antibiotics in the Ballona Wetlands has been confirmed. Additionally, the patterns of frequencies of ABIs occurring have been consistent. As this research is ongoing, the next steps will involve DNA sequencing of the bacteria and identifying the species of prevalent bacteria.

D. Pham (1) and A. Lee (2). 11425 Via Arco, Palos Verdes Estates, CA 90274. 25403 Littlebow Rd., Rancho Palos Verdes, CA 90275. Mentor: John Dorsey, Environmental Science Professor, 1 LMLT Dr., Los Angeles, CA 90045

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Title Annotation:POSTER SESSION ABSTRACTS IN PROGRAM ORDER
Author:Pham, D.; Lee, A.
Publication:Bulletin (Southern California Academy of Sciences)
Article Type:Author abstract
Geographic Code:1USA
Date:Aug 1, 2014
Words:298
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