Determination of the fatty acid profile in Eugenia dysenterica DC. seeds/Determinacao do perfil de acidos graxos em sementes de cagaiteiras (Eugenia dysenterica DC.).
Cagaita (Eugenia dysenterica DC.), one of the native fruits widely used by the regional population, is one of the species that are suffering with the advancement of agribusiness and the urbanization of the midwestern region of the country. It belongs to the Myrtaceae family, measures on average 4-10 m in height and occurs naturally in the states of Sao Paulo, Minas Gerais, Bahia, Tocantins, Mato Grosso, Mato Grosso do Sul, Para, Maranhao, Piaui and Goias, as well as in the Federal District (BRITO et al., 2003).
The main use of cagaita tree is due to the food potential of its fruits. All fruit production occurs in an extractive way, in natural populations of the species, not being recorded any organized planting initiative (VIEIRA et al., 2010). Its fruits can be consumed fresh, or processed in the form of liqueurs, ice creams, juices or jellies, which constitutes an important source of food and possibility of increase in the income of small farmers of the Brazilian midwestern region (CHAVES; TELLES, 2006). The use of this fruit species can constitute a very promising economic activity, given the excellent quality of its fruits and its most diverse uses (SOUZA et al., 2001).
Thus, cagaita is one of the Cerrado species that have great potential for use in agricultural production systems, presenting high density and good production, which favors the exploitation of native populations in a sustainable way. However, there is still a great shortage in researches to overcome restrictive factors that allow the rational use of the species, requiring more in-depth studies in order to better understand this native fruit (VIEIRA et al., 2010).
A segment still unknown to several native cerrado fruits is the use of their vegetable oils, which represent one of the most important resources provided by nature, being produced and stored in fruits and seeds (ATHAR; NASIR, 2005). Oil extracted from fruit seeds can be used in cosmetics and pharmaceuticals, as edible oil, in varnish industries, etc. Therefore, the development of an effective technological extraction and characterization of its physicochemical composition is of utmost importance (ROY et al., 1996).
Recent studies have shown the influence of environmental variation on chemical constituents, including fat percentage, during the production phase of several plant species. According to Kriskamol et al. (2015), the lipid constituents in black gram seeds (Vigna mungo L.) produced in the dry season were higher than in those produced in the rainy season. The amount of rain, sunlight and temperature also affect the chemical compositions of cloudberry (Rubus chamaemorus L.) (JAKAKOL et al., 2012). This influence could also be observed in oil extracted from rapeseed (Brassica napus L.), with variations of oleic and linoleic acid, according to the production season, spring and winter (RAD, ZANDI, 2012). Since it is not synthesized in the body, these components must be provided through meals, and the extraction of constituents in the time of greater production is of extreme importance.
Thus, the aim of this work was to determine the fatty acid profile of cagaita seeds oil grown in the Cerrado of the state of Goias in the of 2013 and 2014 harvests, evaluating the effects of climatic conditions on the synthesis of components.
MATERIAL AND METHODS
Seeds obtained for the research were collected from cagaita trees implanted in the experimental area of the School of Agronomy, Federal University of Goias (EA / UFG), in Goiania-GO. Cagaita trees are 16 years old and were implanted in 1998 using seeds collected from cagaita trees in natural environment in 10 municipalities of the state of Goias. From each locality, 8 to 12 subpopulations were selected, totaling 110 mother plants, which were planted in four blocks, making a total of 440 plants.
From cagaita trees of each block, the maximum number of healthy and good-appearance fruits was collected. Fruits were manually and randomly collected, when fully mature and already fallen to the ground. Collections were carried out in the second fortnight of October 2013 and 2014, season of intense production of fruits.
After pulping, seeds were sent to the Center for Food Research (CPA) of the Veterinary and Animal Science School, Federal University of Goias (EVZ / UFG) to determine the fatty acid profile according to methodology established by the American Oil Chemists' Society--AOCS (AOCS, 1995).
Data obtained were transformed using the formula [square root of] (value + 1), according to Volpato and Barreto (2011) in order to obtain greater homogeneity. Subsequently, they were submitted to analysis of variance (ANOVA), and means were compared by the Tukey test at 5% probability.
Climatic data of temperature (maximum, average and minimum), relative humidity, rainfall and insolation, for the month of October 2013 and 2014, season of cagaita production, were obtained at the First-Class Evaporimetric Station, operating at EA / UFG (Table 1).
Data analysis was performed using the SAS statistical software (2010).
RESULTS AND DISCUSSION
Overall, 37 compounds were identified, including saturated, monounsaturated and polyunsaturated fats. Saturated fats accounted for a total of 27% and unsaturated ones for 73%. Saturated fats presented the highest number of compounds (16); however, when comparing contents, polyunsaturated fats, with 11 compounds, had higher average compared to the others, with 250.036 mg / 100 g, corresponding to a total of 49.65%. There was no variation of the fatty acid profile in cagaita seeds between years 2013 and 2014 (Table 2).
According to Jorge et al. (2010), the fatty acid composition in cagaita seeds had percentage of 37.66% of saturated fatty acids, among which palmitic acid, the most prevalent, with an average of 25.12%.
Analyzing the fatty acid profile of jambolan seeds (Syzygium cumini L.), Luzia and Jorge (2009) obtained 67.91% of the total unsaturated fatty acids, including 24.10% of monounsaturated fatty acids and 43.81% of polyunsaturated acids, with linoleic acid being the major component.
In pequi seeds (Caryocar brasilienses), Damiani et al. (2013) found 86.9% of saturated fatty acids and 10.57% of unsaturated acids. In pitanga seeds, the same family of cagaita, Luzia et al. (2010) obtained 58.06% of the total content of unsaturated fatty acids, among which oleic acid, with average of 38.29%, and linoleic acid, with average of 13.46%. The two major acids accounted for 89% of unsaturated acids, the others appearing in much lower percentages. Among saturated fatty acids, palmitic acid had significant amounts (34.09%).
Among saturated fats, the acid that presented the highest content in the present study was palmitic acid (C16: 0), with average of 105.990 mg / 100 g, followed by butyric acid (C4: 0), with 16.701 mg / 100 g, stearic acid (C18: 0), with 7.977 mg / 100 g, and arachidic, with 2.004 mg / 100 g. The others remained with averages ranging from 0.027 mg / 100 g (caproic acid C10: 0) to 0.677 mg / 100 g (myristic acid C14: 0) (Table 3). In pequi and pitanga seeds, palmitic acid also stood out with higher amounts when compared to the other acids analyzed (LIMA et al., 2007, DAMIANI et al., 2013). This result is consistent with the fact that palmitic acid is the most abundant saturated fatty acid in plant lipids (JORGE; LUZIA, 2012).
According to Sano and Almeida (1998), palmitic acid is a saturated compound, being the main responsible for the high caloric power. Thus, the consumption of products with high content of this acid provides a feeling of fullness due to its high satiety capacity. However, although saturated, stearic fatty acid is not considered atherogenic since it is rapidly converted into oleic acid (monounsaturated) within the organism, which is beneficial to consumers of foods with higher levels of this compound (CASTRO et al., 2004). Oleic acid is not considered essential and can be synthesized by the human organism from saturated fatty acids (MINAZZI-RODRIGUES; PENTEADO, 1991).
Unsaturated fats have positive effects on human health. The consumption of foods with unsaturated fats controls the level of bad cholesterol in the blood and helps to absorb vitamins A, D, E and K (fat soluble vitamins) (HIRAYAMA et al., 2006). Jorge et al. (2010) found in cagaita seeds, amount of unsaturated fatty acids of 62.33%, of which 21.18% are monounsaturated acids and 41.15% are polyunsaturated acids, with linoleic acid being the main component. Among monounsaturated and polyunsaturated acids, only oleic acid (20.17%) and linoleic acid (38.11%) were found in significant amounts.
In monounsaturated fat, oleic acid was obtained in high amounts (Table 4), which is vital in the construction of membranes, being present in the epidermis (outermost skin layer), with the function of protection and barrier against dehydration and also in the formation of hormones (HIRAYAMA et al., 2006).
Other authors also report the presence of high content of oleic acid in pequi, pitanga and jambolan seeds (LUZIA, JORGE, 2009; LUZIA et al., 2010; DAMIANI et al., 2013). In baru almonds, the most frequent fatty acids were oleic (45.577-48.985 g / 100 g) and linoleic acids (22.828-29.707 g / 100 g) (VERA et al, 2009).
In a study carried out by Jardini and Mancini-Filho (2007), the fatty acid profile of oil extracted from pomegranate seeds presented high amount of linoleic and oleic acids, accounting for 70.09% of unsaturated fatty acids, close to values obtained in this study.
In the food industry, oleic acid is the most used in various processes because it provides better quality products. The following are among the various industrial applications: fiber lubricants, high-tech lubricants, cosmetics and chemical intermediates (esters, amines, amides). Pequi pulp and baru almond contain high levels of oleic acid (ALMEIDA et al., 2008).
In polyunsaturated fats, linoleic acid was obtained in greater amount, with 217.164 mg / 100 g, followed by a-linolenic acid, with 14.119 mg / 100 g (Table 5). The former is considered an important anti-inflammatory agent, assists in the body defense, improves the immune system and lowers blood cholesterol. The latter is important for the development of the central nervous and visual systems, as it acts on the formation and regeneration of neurons, decreases the risk of cardiovascular diseases, bad cholesterol and blood sugar, being also an anti-inflammatory agent, and its intake is of great importance for humans (HIRAYAMA et al., 2006).
Linoleic acid is abundantly present in plant seeds and in the oils they produce, such as corn, safflower, cotton, soybean and sunflower oil. Linolenic acid, which is also present in some vegetable oils, although to a lesser extent than linoleic acid, is found in chestnuts and linseed (Hirayama et al., 2006).
Although animal tissues can synthesize most saturated and unsaturated fatty acids, they cannot produce unsaturated fatty acids of linoleic series unless a dietary precursor is provided. If linoleic acid is given in the diet, tissues can synthesize the archonic acid from it. This acid, alone or together with linoleic acid, is essential for the maintenance of the normal skin structure (BURTON, 1979).
The quality and digestibility of edible vegetable oils are determined by the amount and composition of unsaturated fatty acids. The presence of linoleic acid in adequate contents is essential, since it is an essential fatty acid. The higher the amount of linoleic acid in relation to oleic acid, the better the quality of vegetable oil in avoiding the formation of bad cholesterol (EL-ADAWY; TAHA, 2001).
Studying the oil obtained from pitanga seeds, Luzia and Jorge (2009) found oleic / linoleic acid ratio (Ole / Lin) of 1 / 0.35, close to the value found by Borges et al. (2007) for peanut oil (1 / 0.5). In the present study, this ratio was 1 / 1.9, evidencing the high amount of oil, which presents higher levels of linoleic acid in relation to oleic acid. This relationship was lower than that obtained by Jorge et al. (2010) in cagaita seeds, with 1 / 1.89 and higher than that found by Borges et al. (2007) for umbu seeds.
The detailed study of the composition of oil extracted from seeds contributes with professionals of the food area for adequate dietary guidelines, as well as in obtaining data that could be used in composition centesimal tables and of fatty acids and their lipid fraction. Data collection for the composition of Brazilian foods has been stimulated with the objective of obtaining up-to-date, reliable and adequate information to the national reality (LUZIA et al., 2010).
Cagaita seeds presented in their fatty acid profile 73% of unsaturated fats and 27% of saturated fats.
The major fatty acids present in the seeds were linoleic acid, oleic acid and palmitic acid.
There was no variation in the fatty acid composition present in seeds between years 2013 and 2014, therefore concluding that there are no external influences such as climate or foliar or soil nutrients in the production of these oils.
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YANUZI MARA VARGAS CAMILO (2), ELI REGINA BARBOZA DE SOUZA (3), RONALDO VELOSO NAVES (3), ROSANGELA VERA (3), MUZA DO CARMO VIEIRA (4)
(1) (Paper 144-15). Received May 28, 2015. Accepted November 03, 2015. Part of the doctoral thesis of the first author.
(2) PhD student in Agronomy, School of Agronomy, Federal University of Goias (EA / UFG), Goiania-GO, Brazil. Email: firstname.lastname@example.org
(3) Professor at the School of Agronomy, Federal University of Goias (EA / UFG), Goiania-GO, Brazil. Email: email@example.com, firstname.lastname@example.org; email@example.com
(4) Agronomist, PhD in Agronomy--Federal Institute of Goias--Urutai Campus--Goias--Brazil. Email: firstname.lastname@example.org Financial Support: National Council for Scientific and Technological Development--CNPq.
TABLE 1--Mean data of temperature, relative humidity, rainfall and insolation in the region of the School of Agronomy (UFG), Goiania-GO, in October 2013 and 2014. Temperature ([degrees]C) Month Min. Interm. Max. RH% Rainfall Insolation (mm) (hour) October/2013 19.3 25.6 32.0 55 165.5 183.6 October/2014 18.1 26.1 34 59 53.5 218.4 TABLE 2--Analysis of variance for saturated, monounsaturated and polyunsaturated fats identified in cagaita seeds (Eugenia dysenterica) grown in the experimental area of the School of Agronomy, Federal University of Goias, Goiania-GO, harvests of 2013 and 2014. Source of Saturated Monounsaturated Polyunsaturated variation Fats (mg/ Fats (mg/100 g) Fats (mg/100 g) 100 g) Year 4.249 (NS) 2.836 (NS) 4.814 (NS) Mean 135.967 117.56 250.036 VC% 28.03 51.46 53.26 NS-Not Significant. VC-variation coefficient TABLE 3--Analysis of variance for the saturated fat content (mg -100 g) present in cagaita seeds (Eugenia dysenterica) grown in the experimental area of the School of Agronomy, Federal University of Goias, Goiania-GO, harvests of 2013 and 2014. Source of Butyric acid Caproic acid Caprylic acid Variation C4:0 C6:0 C8:0 Year 7.665 (NS) 4.832 (NS) 0.875 (NS) Mean 16.701 0.086 0.067 VC (%) 69.35 4.99 6.49 Source of Myristic Acid Pentadecylic Acid Palmitic Acid Variation C14:0 C15:0 C16:0 Year 2.142 (NS) 0.835 (NS) 8.297 (NS) Mean 0.677 0.126 105.990 VC (%) 19.27 6.21 32.06 Source of Arachidonic Heneicosanoic Behenic acid Variation Acid C20:0 Acid C21:0 C22:0 Year 6.431 (NS) 1.818 (NS) 0.128 (NS) Mean 2.004 0.394 0.436 VC (%) 33.15 17.43 15.39 Source of Capric acid Hendecanoic acid Lauric acid Variation C10:0 C11:0 C12:0 Year 1.000 (NS) 0.673 (NS) 0,407 (NS) Mean 0.027 0.084 0,361 VC (%) 24.80 9.51 26,57 Source of Margaric Stearic acid Variation Acid C17:0 C18:0 Year 1.170 (NS) 7.421 (NS) Mean 0.213 7.977 VC (%) 6.22 22.87 Source of Tricosanoic acid Lignoceric acid Variation C23:0 C24:0 Year 0.822 (NS) 0.029 (NS) Mean 0.411 0.413 VC (%) 26.03 25.60 NS-Not Significant. VC-variation coefficient TABLE 4--Analysis of variance for monounsaturated fats (mg -100 g), present in cagaita seeds (Eugenia dysenterica) grown in the experimental area of the School of Agronomy, Federal University of Goias, Goiania-GO, harvests of 2013 and 2014. Source of Myristoleic Acid C 15:1 N3 Palmitoleic acid Variation C14:1 N5 C16:1N9 Year 2.359 (NS) 2.882 (NS) 2.092 (NS) Mean 0.005 0.012 0.870 VC (%) 0.44 1.01 20.30 Source of Oleic Acid C20:1 N9 C22:1 N9 Variation C18:1 N9 Year 0.347 (NS) 0.697 (NS) 0.041 (NS) Mean 113.057 2.156 0.005 VC (%) 86.62 70.60 0.35 Source of Palmitoleate acid C17:1 N7 Variation C16:1 N7 Year 4.351 (NS) 0.587 (NS) Mean 0.081 1.084 VC (%) 4.98 53.64 Source of Nervonic acid Variation C24:1 N9 Year 2.010 (NS) Mean 0.290 VC (%) 15.00 TABLE 5--Analysis of variance for the polyunsaturated fats content (mg -100 g), present in cagaita seeds (Eugenia dysenterica) grown in the experimental area of the School of Agronomy, Federal University of Goias, Goiania-GO, harvests of 2013 and 2014. Source of Linoleic Acid Linolelaidic acid [alpha]-Linolenic Variation C18:2 N6 C18:2N6 trans acid C18:3 N3 Year 5.626 (NS) 0.859 (NS) 0.797 (NS) Mean 217.164 0.958 14.119 VC (%) 49.39 50.61 54.77 Source of C20:2 Dihomo-([alpha]) Dihomo-[gamma]- Variation linolenic acid linolenic acid C20:3 N3 C20:3 N6 Year 0.003 (NS) 1.000 (NS) 2.291 (NS) Mean 0.252 0.009 0.366 VC (%) 7.23 0.49 26.67 Source of C20:4 N9 C22:2 N6 DHA C22:6 N3 Variation Year 1.485 (NS) 1.684 (NS) 0.949 (NS) Mean 5.870 0.012 10.369 VC (%) 160.56 1.30 139.06 Source of [gamma]-linolenic Variation acid C18:3 N6 Year 0.127 (NS) Mean 0.759 VC (%) 56.17 Source of Arachidonic Variation acid C20:4 N6 Year 3.963 (NS) Mean 0.158 VC (%) 8.92 Source of Variation Year Mean VC (%)