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Determination of the Phenolic Profile and Antioxidant Activity of Leaves and Fruits of Spanish Quercus coccifera.

1. Introduction

Plants represent a rich source of natural compounds which are responsible for many multifunctional biological effects. In the last few years, exhaustive research is being carried out to obtain new raw materials from plants for the development of products with healthy characteristics, which help maintain or improve health and protect against chronic diseases.

The genus Quercus (Fagaceae) has 450 species estimated worldwide [1] and has been widely investigated for years, not only due to their extensive use in the wine and wood industries but also for animal feeding and medicinal purposes [2]. Acorns of Quercus species are a high-protein food source for a wide array of wildlife and are also used to fatten poultry and pigs [3, 4]. They are not only a source of important nutrients, namely carbohydrates, proteins, fatty acids, and sterols [5, 6], but also of phenolic constituents [3, 6, 7]. On the contrary, leaves from different Quercus species, which are commonly consumed as tisanes (aqueous extracts), also contain bioactive compounds, in particular phenolics [1, 8]. The antioxidant and biological activities of extracts of leaves [9, 10], acorns [4, 10, 11], and other different morphological parts of Quercus species such as twigs and cork [12] have been evaluated. Quercus species have been reported to have gastroprotective [13], antibacterial [14], cardioprotective [15], hepatoprotective [15], anti-inflammatory [16], and anticarcinogenic [16] effects, among other health benefits.

These biological activities are thought to be associated, at least in part, with the presence of phenolic compounds, such as flavonoids and tannins [3,4, 11,17]. The phenolic profiles vary significantly among Quercus species. For instance, high levels of gentisic and chlorogenic acids, as well as of the flavonoids naringin and rutin, have been found in Quercus acuta, Quercus glauca, Quercus myrsinifolia, Quercus phylliraeoides, and Quercus salicina [18]. Nevertheless, none of these compounds was detected in any other Quercus species [6]. Likewise, several gallic acid derivatives have been solely found in Quercus ilex, Quercus rotundifolia, and Quercus suber [3]. Despite the phylogenetic variability, flavonoids, phenolic acids, and tannins are somehow ubiquitous in all Quercus species [6]. High levels of ellagitannins, a group of condensed tannins, have been reported in woods and barks of several Quercus species used in cooperages [19]. Gallotannins, another essential group of tannins, have also been reported in extracts of Quercus species [1, 20]. Other phenolic compounds such as flavonoids of quercetin and kaempferol have been found in Quercus leaves [1].

Kermes oak (Quercus coccifera L.) is a small evergreen shrub of fewer than 2 meters, whose fruits are acorns provided with stings. Q. coccifera is the prevailing species in the evergreen sclerophyllous shrublands, which are an important part of Mediterranean rangelands. Despite its low commercial value with regard to wood production, it plays a significant role in preventing soil erosion [21], and it is used for fodder production for domestic and wild animals [22]. Kermes oak acorns seem to be a highly energetic resource for small ruminants such as goats and lambs and are often compared to barley [23, 24].

Several biological effects have been reported for Q. coccifera such as neuroprotective [25], antibacterial [26, 27], antifungal [26], antihelmintic [28], and antioxidant [29] activity. Furthermore, previous studies have shown that Q. coccifera contains tocopherols and fatty acids [7], besides phenolic compounds such as tannins and flavonoids [27, 29]. In a recent article [30], the authors characterized individual phenolics in fruits and leaves of Q. coccifera, although of only seven compounds. Phenolic compounds have been shown to be responsible for many health benefits [6] and are very useful in the food industry since they can be used as dietary supplements or as preservatives instead of synthetic antioxidants such as butylhydroxytoluene (BHT) and butylhydroxyanisole (BHA), which have negative effects on human health. Therefore, the leaf and acorn extracts can represent a valuable source of natural antioxidants for different applications. The extracts can be obtained by using simple extraction procedures directly from the raw material, reducing the need for additional processing stages.

Considering the variety of phenolic compounds that have been reported in different Quercus species and that the published research concerning phenolics in Q. coccifera is scarce, our work aimed at identifying the extractable phenolic compounds present in acorns and leaves from Q. coccifera and their antioxidant activity. These data provide a better understanding of the composition of this Quercus species and can lead to further investigations regarding the valorization of its residues and the use of its biomass, within a biorefinery concept, for the production of biofuels; chemical, pharmaceutical, and care products; and bioenergy. The exploitation of leaves and acorns from Q. coccifera on an industrial scale could contribute to improving the sustainability of the agrofood chain by achieving new food products or as alternative sources of different highly-valued food ingredients. The upgrading of these products of the forest industry is an important challenge in the development of a sustainable economy and environmental friendly industrial processes.

2. Materials and Methods

2.1. Reagents and Solutions. All reagents and standards were of analytical grade unless stated otherwise. Activated charcoal (p.a.), catechin ([greater than or equal to] 99%), citric acid ([greater than or equal to] 99.5%), gallic acid ([greater than or equal to] 99%), procyanidin B2 ([greater than or equal to] 90%), kaempferol ([greater than or equal to] 97%), quercetin ([greater than or equal to] 95%), 2,2'-azinobis(3-ethylbenzthiazoline-6- sulfonic acid) (ABTS, [greater than or equal to] 98%), potassium persulfate ([K.sub.2][S.sub.2][O.sub.8], >99%), 6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid (Trolox, 97%), and 2,2-diphenyl-l-picrylhydrazyl (DPPH, 95%) were purchased from Sigma-Aldrich (Madrid, Spain). Methanol (MeOH [greater than or equal to] 99%), ethanol (96%), Folin-Ciocalteu's phenol reagent (FCR), and sodium carbonate ([Na.sub.2]C[O.sub.3]) (p.a.) were purchased from Panreac (Barcelona, Spain). LC-MS grade acetonitrile (C[H.sub.3]CN) (99%) (Sigma-Aldrich) and ultrapure water (Milli-Q Waters purification system; Millipore; Milford, MA) were also used.

2.2. Sample Collection and Preparation. Plant materials were collected from different plants placed in Sierra Morena, a mountain range in the province of Jaen, Andalucia, southcentral Spain. The materials were collected in October 2016, at an approximate height of 550 meters (38[degrees]08'01.97" N, 3[degrees]58'30.03"W). For analysis, we separated plant materials into leaves and acorns (fully ripe) and analyzed all samples as the same batch. They were lyophilized to dryness (ModulyoD freeze dryer; Thermo Fisher Scientific, Madrid, Spain), ground to powder, and stored at -20[degrees]C.

For the extraction of the phenolic compounds, we carried out an ultrasound-assisted solid-liquid extraction using 5g of sample powder and 100mL of MeOH using a sonicator with a temperature controller (Bandelin Sonorex Digital lOP; Sigma-Aldrich, Madrid, Spain) at 35 Hz and 280 W for 60 min (room temperature). Then, we filtered the extracts, eliminated the chlorophylls by adsorption on activated charcoal, and concentrated the extracts to dryness using a rotary evaporator (Buchi Rotavapor R-114) at 40[degrees]C. The resulting extracts were stored at 4[degrees]C until analysis.

2.3. HPLC Analysis. The analysis of the phytochemical profile was carried out by using [HPLC-MS.sup.n]. Dried extract (DE) of 5-10mg was re-dissolved in lmL MeOH. After filtration through 0.45 [micro]m PTFE membrane filters, 10 [micro]L of each solution was injected in the chromatographic system.

An Agilent Series 1100 HPLC system (Agilent Series 1100, Agilent Technologies, Santa Clara, CA, USA) with a G1315B diode array detector was used. A reversed-phase Kinetex core-shell [C.sub.18] analytical column of 100 x 2.1mm and 2.6 [micro]m particle size (Phenomenex, Torrance, CA, USA) and a [C.sub.18] Security Guard Ultra cartridge (Phenomenex) of 2.1 mm i.d. placed before the analytical column were used. The mobile phase consisted of acetonitrile (C[H.sub.3]CN) and water-formic acid (100:0.1, v/v). The following gradients were used: initial mobile phase, 10% C[H.sub.3]CN; linear increase to 25% C[H.sub.3]CN (0-10 min); 25% C[H.sub.3]CN (10-20 min); linear increase to 50% C[H.sub.3]CN (20-40min); linear increase to 100% C[H.sub.3]CN (40-42min); and return to initial mobile phase and stabilization time of 7 min. The mobile phase flow rate was 0.4 mL x [min.sup.-1].

The HPLC system was connected to an ion trap mass spectrometer (Esquire 6000, Bruker Daltonics, Billerica, MA, USA) equipped with an electrospray (ESI) interface. The scan range was set at m/z 100-1200 with a speed of 13,000 Da/s. The ESI conditions were as follows: drying gas ([N.sub.2]) flow rate and temperature, 10mL/min and 3 650C; nebulizer gas ([N.sub.2]) pressure, 50 psi; capillary voltage, 4500 V; and capillary exit voltage, -117.3 V. We used the auto [MS.sup.n] mode (negative and positive modes) for the acquisition, with isolation width of 4.0 m/z, and fragmentation amplitude of 0.6 V ([MS.sup.n] up to [MS.sup.4]). The analysis of the phenolic composition was performed with HPLC-ESI-[MS.sup.n] using negative ionization mode.

2.4. Total Phenolic and Flavonoid Contents and Antioxidant Capacity Assays. The total phenolic (TPC) and flavonoid (TFC) contents were obtained using the Folin-Ciocalteu and aluminum chloride methods, respectively. TPC was expressed as mg of gallic acid equivalents (GAE) per g of DE. TFC was expressed as mg of quercetin equivalents (QE) per g of DE. [ABTS.sup.+] and DPPH radical scavenging activities were expressed as [micro]mol Trolox equivalent (TE) per 100g of DE. Detailed procedures have been previously reported [31].

3. Results and Discussion

Currently, there is scarce information concerning Q. coccifera, as most of the published articles regarding the Quercus genus have focused on Q. suber due to its extensive use in cork industry. For Q. coccifera, water and methanol [29] and water with mixtures of acetone, ethyl acetate, and methanol [27] have been used as extraction solvents. For Q. suber, different extraction solvents and temperature have been reported: water at 80[degrees]C [1], 100% methanol and 80% methanol: water [12], or methanol and hexane [10]. Hence, it is clear that there is no standard protocol to carry out the extraction of phenolics, as they have very different polarities. In this work, we have selected methanol--one of the most common extractants [6]--to carry out the extraction procedure. We performed an ultrasound-assisted solid-liquid extraction, whose main benefits are its simplicity and rapidity.

3.1. Phytochemical Profile. The initial step for the characterization of the compounds consisted in the determination of the molecular weight of each compound. In the negative ion mode MS1 spectrum, the most intense peak corresponded to the deprotonated molecular ion [[M - H].sup.-] or formate adduct [[M + HCOOH - H].sup.-]. The base peak chromatograms of extracts of acorns and leaves are shown in Figure 1, whereas the MS data for the detected compounds are reported in Table 1.

3.1.1. Tannins and Gallic Acid Derivatives. Most of the compounds found in the extracts of Q. coccifera corresponded to tannins, which can be classified in proanthocyanidins, ellagitannins, and gallotannins (galloylglucoses).

Compound 6 was a procyanidin dimer, (epi)catechin(epi)catechin, B-type [32]. With an additional 152 Da (galloyl moiety), we characterized compound 17 as (epi) catechin-(epi)catechin monogallate.

Twelve gallotannins, monomers and oligomers of gallic acid with a hexoside moiety, were characterized in the analyzed extracts. These compounds were characterized by the neutral losses of 152 Da (galloyl moiety; typical 169 [right arrow] 125 fragmentation) and 162 Da (hexoside moiety). Compound 4 was the only monogalloyl-hexoside. Compounds 5 and 7 were digalloyl-hexosides [33]. Five trigalloylhexosides were characterized (compounds 9,12,16,18, and 19); compound 25 was a tetragalloy-hexoside; compounds 27 and 28 were pentagalloyl-hexosides [20], and compound 33 was a hexagalloyl-hexoside [34].

Compounds 8 and 13 were tentatively characterized as isomers of hexahydroxydiphenyl-digalloyl-glucose based on their molecular weight and fragment ions at m/z 301 (loss of a digalloyl-hexoside residue) and 483 (loss of a hexahydroxydiphenyl residue) [20].

The fragmentation of compound 11, with [[M - H].sup.-] at m/z 183, was consistent with methyl gallate [35].

Compound 20, with [[M - H].sup.-] at m/z 473, suffered two consecutive losses of 152 Da (galloyl moieties) to yield gallic acid at m/z 169, fragmentation that corresponded to trigallic acid.

Compounds 22 and 29, which suffered neutral losses of 152 and 15 Da, were characterized as galloyl methyl gallate isomers [36].

Compound 23 exhibited the deprotonated molecular ion at m/z 615. After the neutral losses of 152 Da (galloyl) and 162 Da (hexoside), it yielded a fragment ion at m/z 301, corresponding to quercetin. Hence, we identified this compound as quercetin hexoside-gallate.

Compound 26, [[M - H].sup.-] at m/z441, suffered the neutral loss of a galloyl moiety to yield (epi)catechin at m/z 289, so we characterized it as (epi) catechin-O-gallate.

Similar to the reported results, other authors found abundance of galloyl derivatives in different Quercus species [1, 20]. For instance, mono-, di-, tri-, tetra-, and pentagalloyl glucosides have been found in Q. ilex, Q. suber, and Q. rotundifolia [6].

3.1.2. Flavonoids. Compound 10 was identified as catechin by comparison with an analytical standard. This compound has already been reported in several Quercus species, such as Q. acuta, Q. salicina, and Quercus resinosa, among others [6].

Three kaempferol derivatives were present in the extracts. The aglycone at m/z285 was identified by comparison with an analytical standard. Compound 38 suffered the neutral loss of 308Da (rutinoside), whereas compound 43 displayed the neutral loss of 146 (rhamnoside) and 308 Da. The exact nature of compound 44 could not be completely elucidated.

Compound 41 and 42 were characterized as quercetin-rhamnoside-hexoside-rhamnoside isomers based on the neutral losses of rhamnoside (146 Da) and hexoside (162 Da) moieties, and the aglycone quercetin observed at m/z 301 (comparison with an analytical standard).

The presence of kaempferol derivatives and quercetin derivatives has previously been reported in the leaves of different Quercus species [1], although mono- and diglycosides were detected, not tri-glycosides, which are here reported for the first time to the best of our knowledge.

3.1.3. Other Compounds. Compound 1 was characterized as a quinic acid derivative due to the 191 [right arrow] 127 fragmentation.

Compound 2 was identified as a disaccharide, whereas compounds 24 and 32 were tentatively characterized as saccharide derivatives due to the [MS.sup.n] fragment ions at m/z 179, 161, 143, 119, and 113 [37].

Compound 3 was identified as citric acid by comparison with an analytical standard.

Compound 14, with a deprotonated molecular ion at m/z 387, displayed fragment ions at m/z 207 and 163, characteristic of the lignan medioresinol [38].

Compound 15 was characterized as roseoside (formate adduct) due to the deprotonated molecular ion at m/z 385 and fragment ions at m/z 223 and 153 [31].

We characterized compounds 39 and 40 as oxodihydroxy-octadecenoic and trihydroxy-octadecenoic acids, respectively, after comparison with bibliographic data [39, 40].

3.2. (Semi)quantification of Phenolics. We performed the semiquantitative analysis of the main compounds found in leaves and acorns. Acorn extracts were rich in gallic acid derivatives. Hence, the quantification of these compounds was carried out using gallic acid to construct the analytical graph, using the UV chromatograph at 275 nm. For leaf extracts, we used the UV signal of analytical standards of catechin (280nm), gallic acid (275 nm), quercetin (350 nm), and kaempferol (350 nm) to construct the calibration graphs. The results are summarized in Table 2. These results are expressed in mg/g DE. In addition, humidity percentages were also calculated (62 [+ or -] 2% and 58 [+ or -] 3% for acorns and leaves, resp.), so that the amounts of phenolics in fresh samples could be calculated too.

To the best of our knowledge, the quantification of individual phenolics has not been reported for Q. coccifera. However, the results can be compared with data from other Quercus species, always keeping in mind that different solvents and extraction procedures have been used. Garcia-Villaba et al. [1] analyzed leaves of seven Quercus species, obtaining 1-5 mg/g DE of flavonoids and 2.5-285 mg/g DE of hydrolyzable tannins. In this work, we observed higher amounts of flavonoids in leaves, and the amounts of gallic acid derivatives are within the concentrations found by the mentioned authors.

3.3. Total Phenolic Content and Antioxidant Assays. We determined the total phenolic content (TPC) and the antioxidant activity ([ABTS.sup.+] and DPPH) of the extracts using the procedures previously reported [31]. The results are depicted in Figure 2. It can be observed that very high values of TPC were observed in both acorns and leaves, although acorns had higher TPC values than leaves. High TPC values (similar to the ones observed here in leaves) have been previously reported in Q. suber [12]. In a similar way to TPC, the values obtained for the [ABTS.sup.+] and DPPH antioxidant assays were also higher in acorns compared to leaves.

4. Conclusions

We have carried out the characterization of the phenolic profile of methanolic extracts of leaves and acorns of Q. coccifera by using [HPLC-ESI-MS.sup.n]. A total of forty-one compounds were identified or tentatively characterized. Although some flavonoids were identified, mainly kaempferol and quercetin derivatives, most of the compounds were condensed tannins and gallic acid derivatives. The extracts were particularly rich in gallotannins, which is in line with the reports in other Quercus species. The phytochemical profiles of acorns and leaves were similar, although (epi) catechin dimers were only detected in leaves, and hexahydroxydiphenyl-digalloyl-glucose isomers were only present in acorns. The main compounds were quantified by using HPLC with UV detection. Both acorns and leaves had high antioxidant potential, which was in agreement with the TPC values observed, particularly in acorns. This study provides additional information concerning the phytochemical profile of this plant, which can be a valuable source of phytochemicals for the food or pharmacological industries. However, it is important to mention that these results are representative of the studied area, but samples were collected only in one year. Hence, more results are required considering that they may vary within different years or collection places.

https://doi.org/10.1155/2018/2573270

Data Availability

The HPLC-MS data used to support the findings of this study are available from the corresponding author upon request.

Conflicts of Interest

The authors declare that there are no conflicts of interest regarding the publication of this paper.

Acknowledgments

Technical and human support provided by CICT of Universidad de Jaen (UJA, MINECO, Junta de Andalucia, FEDER) is gratefully acknowledged. This research was supported by funding from the University of Jaen (UJA2014/10_FT/01).

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L. Molina-Garcia, R. Martinez-Exposito, M. L. Fernandez-de Cordova, and E. J. Llorent-Martinez (iD)

Department of Physical and Analytical Chemistry, Faculty of Experimental Sciences, University of Jaen, Campus Las Lagunillas, E-23071 Jaen, Spain

Correspondence should be addressed to E. J. Llorent-Martinez; ellorent@ujaen.es

Received 30 May 2018; Revised 24 July 2018; Accepted 9 August 2018; Published 6 September 2018

Academic Editor: Jose A. Pereira

Caption: Figure 1: HPLC-ESI/[MS.sup.n] base peak chromatograms (BPCs) of the methanolic extracts of acorns and leaves of Q. coccifera.

Caption: Figure 2: TPC, ABTS, and DPPH results for acorns and leaves of methanol extracts of Q. coccifera. GAE: gallic acid equivalents; TE: Trolox equivalents; DE: dried extract.
Table 1: Characterization of the compounds found in the methanolic
extracts of acorns and leaves of Q. coccifera.

No.   [t.sub.R] (min)   [[M - H].sup.-] m/z

1           1.1                 533
2           i.i                 341
3           1.2                 191
4           1.3                 331
5           1.6                 483
6           2.1                 577
7           2.2                 483
8           2.5                 785
9           2.6                 635
10          2.9                 289
11          3.3                 183
12          3.6                 635
13          3.9                 785
14          4.3                 387
15          4.4                 431
16          4.6                 635
17          5.1                 729
18          5.2                 635
19          5.7                 635
20          6.5                 473
21          7.3                 345
22          7.4                 335
23          7.7                 615
24          7.9                 377
25          8.0                 787
26          8.5                 441
27          8.9                 937
28          9.6                 939
29          9.6                 335
30          9.9                 599
31         10.3                 939
32         10.5                 417
33         11.0                1091
34         12.8                 487
35         14.5                 639
36         14.8                 507
37         15.1                 493
38         16.8                 593
39         20.5                 327
40         26.1                 329
41         27.5                 755
42         28.9                 755
43         30.2                 739
44         35.1                 781

No.                          m/z (% base peak)

1                       [MS.sup.2] [533]: 191 (100)
               [MS.sup.3] [533 [right arrow] 191]: 127(100)
           [MS.sup.2] [341]: 179 (100), 161 (20), 143 (25), 119
2                              (11), 113 (6)
          [MS.sup.3] [341 [right arrow] 179]: 161 (26), 149 (100)
3                  [MS.sup.2] [191]: 171 (27), 111 (100)
4             [MS.sup.2] [331]: 169 (100), 271 (43), 211 (40)
               [MS.sup.3] [331 [right arrow] 169]: 125 (100)
               [MS.sup.2] [483]: 331 (100), 313 (2), 169 (8)
5       [MS.sup.3] [483 [right arrow] 331]: 271 (26), 211 (19), 193
                         (18), 169 (100), 125 (18)
6          [MS.sup.2] [577]: 451 (33), 425 (100), 407 (66), 289
                              (26), 287 (13)
           [MS.sup.2] [483]: 331 (27.8), 313 (24.8), 271 (100),
7                               211 (15.8)
          [MS.sup.3] [483 [right arrow] 271]: 211 (100), 169 (20)
           [MS.sup.2] [785]: 785 (100), 633 (19), 483 (19), 3O1
8                                  (53)
          [MS.sup.3] [785 [right arrow] 301]: 257 (32), 229 (100)
              [MS.sup.2] [635]: 483 (30), 465 (100), 313 (9)
9         [MS.sup.3] [635 [right arrow] 465]: 421 (45), 313 (47),
                                 169 (100)
      [MS.sup.4] [635 [right arrow] 465 [right arrow] 169]: 125 (100)
10         [MS.sup.2] [289]: 245 (100), 2O5 (34), 203 (29), 179
                                   (11)
11                      [MS.sup.2] [183]: 168 (100)
               [MS.sup.3] [183 [right arrow] 168]: 124 (100)
              [MS.sup.2] [635]: 483 (100), 465 (6), 331 (25)
12    [MS.sup.3] [635 [right arrow] 483]: 331 (100), 313 (3), 169 (3)
      [MS.sup.4] [635 [right arrow] 483 [right arrow] 331]: 271 (48),
                       211 (20), 169 (100), 125 (10)
           [MS.sup.2] [785]: 785 (100), 633 (13), 483 (51), 301
                                   (77)
13            [MS.sup.3] [785 [right arrow] 301]: 257 (26.5)
        [MS.sup.3] [785 [right arrow] 483]: 313 (59), 295 (30), 193
                              (40), 169 (100)
14                 [MS.sup.2] [387]: 207 (100), 163 (67)
               [MS.sup.3] [387 [right arrow] 207]: 163 (100)
                   [MS.sup.2] [431]: 385 (100), 161 (9)
15     [MS.sup.3] [431 [right arrow] 385]: 223 (48), 205 (68), 161
                              (44), 153 (100)
               [MS.sup.2] [635]: 483 (4), 465 (100), 313 (3)
16       [MS.sup.3] [635 [right arrow] 465]: 313 (100), 295 (21),
           [MS.sup.4] [635 [right arrow] 465 [right arrow] 313]:
                            295 (25), 169 (100)
              [MS.sup.2] [729]: 577 (100), 425 (38), 407 (45)
17      [MS.sup.3] [729 [right arrow] 577]: 451 (63), 425 (42), 407
                         (100), 289 (31), 287 (17)
                   [MS.sup.2] [635]: 483 (100), 465 (80)
        [MS.sup.3] [635 [right arrow] 483]: 331 (33), 313 (10), 271
18                       (100), 211 (13), 169 (20)
         [MS.sup.4] [635 [right arrow] 483 [right arrow] 271]: 211
                         (100), 169 (26), 125 (3)
                        [MS.sup.2] [635]: 483 (100)
        [MS.sup.3] [635 [right arrow] 483]: 331 (10), 313 (15), 271
                                  (100),
19                               211 (38)
         [MS.sup.4] [635 [right arrow] 483 [right arrow] 271]: 211
                         (100), 169 (26), 125 (15)
                   [MS.sup.2] [473]: 321 (100), 169 (14)
20        [MS.sup.3] [473 [right arrow] 321]: 169 (100), 125 (8)
      [MS.sup.4] [473 [right arrow] 321 [right arrow] 169]: 125 (100)
21                      [MS.sup.2] [345]: 183 (100)
      [MS.sup.3] [345 [right arrow] 183]: 165 (100), 139 (85), 121 (78)
22                      [MS.sup.2] [335]: 183 (100)
      [MS.sup.3] [335 [right arrow] 183]: 183 (100), 168 (21), 124 (32)
                   [MS.sup.2] [615]: 463 (100), 301 (36)
23        [MS.sup.3] [615 [right arrow] 463]: 301 (100), 179 (11)
         [MS.sup.4] [615 [right arrow] 463 [right arrow] 301]: 255
                         (100), 179 (62), 151 (62)
                   [MS.sup.2] [377]: 331 (100), 179 (14)
24     [MS.sup.3] [377 [right arrow] 331]: 179 (100), 161 (34), 143
                         (34), 119 (19), 113 (20)
           [MS.sup.2] [787]: 635 (100), 617 (65), 483 (15), 465
25                                  (8)
          [MS.sup.3] [787 [right arrow] 635]: 483 (100), 313 (5)
          [MS.sup.3] [787 [right arrow] 617]: 573 (49), 465 (69),
                                 331 (100)
26      [MS.sup.2] [441]: 289 (100), 271 (10), 169 (23) [MS.sup.3]
          [441 [right arrow] 289]: 245 (100), 203 (64), 179 (34)
27                      [MS.sup.2] [939]: 769 (100)
               [MS.sup.3] [939 [right arrow] 769]: 617 (100)
           [MS.sup.2] [939]: 787 (100), 769 (97), 635 (20), 617
                                    (9)
28     [MS.sup.3] [939 [right arrow] 787]: 635 (56), 617 (100), 465
                               (19), 447 (6)
      [MS.sup.4] [939 [right arrow] 787 [right arrow] 617]: 447 (100)
29                      [MS.sup.2] [335]: 183 (100)
         [MS.sup.3] [335 [right arrow] 183]: 183 (100), 168 (21),
                                 124 (32)
                        [MS.sup.2] [599]: 313 (100)
30             [MS.sup.3] [599 [right arrow] 313]: 169 (100)
      [MS.sup.4] [599 [right arrow] 313 [right arrow] 169]: 125 (100)
31            [MS.sup.2] [939]: 787 (100), 769 (3) [MS.sup.3]
               [939 [right arrow] 787]: 635 (100), 617 (95)
32                      [MS.sup.2] [417]: 371 (100)
         [MS.sup.3] [417 [right arrow] 371]: 161 (100), 179 (15),
                                 113 (18)
                   [MS.sup.2] [1091]: 939 (100), 787 (5)
33       [MS.sup.3] [1091 [right arrow] 939]: 787 (24), 769 (100)
        [MS.sup.4] [1091 [right arrow] 939 [right arrow] 769]: 617
                              (44), 447 (100)
                   [MS.sup.2] [487]: 335 (100), 183 (11)
34             [MS.sup.3] [487 [right arrow] 335]: 183 (100)
         [MS.sup.4] [487 [right arrow] 335 [right arrow] 183]: 168
                              (29), 124 (100)
                   [MS.sup.2] [639]: 487 (100), 335 (48)
35        [MS.sup.3] [639 [right arrow] 487]: 335 (100), 183 (12)
      [MS.sup.4] [639 [right arrow] 487 [right arrow] 335]: 183 (100)
                   [MS.sup.2] [507]: 461 (100), 293 (45)
36        [MS.sup.3] [507 [right arrow] 461]: 293 (100), 149 (17)
      [MS.sup.4] [507 [right arrow] 461 [right arrow] 293]: 125 (100)
                        [MS.sup.2] [493]: 447 (100)
37      [MS.sup.3] [493 [right arrow] 447]: 191 (18), 165 (18), 149
                              (100), 131 (32)
         [MS.sup.4] [493 [right arrow] 447 [right arrow] 149]: 131
                              (100), 113 (18)
38                      [MS.sup.2] [593]: 285 (100)
          [MS.sup.3] [593 [right arrow] 285]: 257 (100), 151 (18)
39         [MS.sup.2] [327]: 291 (54), 229 (100), 211 (48), 209
                              (40), 171 (99)
40          [MS.sup.2] [329]: 311 (36), 293 (28), 229 (98), 211
                              (100), 171 (25)
                   [MS.sup.2] [755]: 609 (100), 301 (87)
41        [MS.sup.3] [755 [right arrow] 609]: 463 (87), 301 (100)
      [MS.sup.4] [755 [right arrow] 609 [right arrow] 301]: 179 (100)
                   [MS.sup.2] [755]: 609 (100), 301 (67)
42        [MS.sup.3] [755 [right arrow] 609]: 463 (84), 301 (100)
      [MS.sup.4] [755 [right arrow] 609 [right arrow] 301]: 179 (100)
43            [MS.sup.2] [739]: 593 (37), 453 (63), 285 (100)
          [MS.sup.3] [739 [right arrow] 285]: 255 (100), 151 (55)
44            [MS.sup.2] [781]: 635 (25), 495 (40), 285 (100)
               [MS.sup.3] [781 [right arrow] 285]: 151 (100)

No.           Assigned identification            Acorns    Leaves

1              Quinic acid derivative            [check]
2                   Disaccharide                 [check]   [check]
3                   Citric acid                  [check]   [check]
4                 Galloyl-hexoside               [check]   [check]
5                Digalloyl-hexoside              [check]   [check]
6                Procyanidin dimer                         [check]
7                Digalloyl-hexoside              [check]   [check]
8      Hexahydroxydiphenyl-digalloyl-glucose     [check]
9               Trigalloyl-hexoside              [check]
10                    Catechin                   [check]   [check]
11                 Methyl gallate                [check]
12              Trigalloyl-hexoside              [check]   [check]
13     Hexahydroxydiphenyl-digalloyl-glucose     [check]
14                  Medioresinol                 [check]
15           Roseoside (formate adduct)                    [check]
16              Trigalloyl-hexoside              [check]
17    (Epi)catechin-(epi)catechin monogallate              [check]
                      (B-type)
18              Trigalloyl-hexoside              [check]   [check]
19              Trigalloyl-hexoside              [check]
20                 Trigallic acid                [check]
21                    Unknown                              [check]
22             Galloyl methyl gallate            [check]
23           Quercetin hexoside-gallate                    [check]
24             Saccharide derivative                       [check]
25             Tetragalloyl-hexoside             [check]
26            (epi)catechin- O-gallate                     [check]
27             Pentagalloyl-hexoside             [check]   [check]
28             Pentagalloyl-hexoside             [check]
29             Galloyl methyl gallate            [check]
30             Gallic acid derivative                      [check]
31             Pentagalloyl-hexoside             [check]
32             Saccharide derivative                       [check]
33              Hexagalloyl-hexoside             [check]
34            Digalloyl methyl gallate           [check]
35           Trigalloyl methyl gallate           [check]
36                    Unknown                              [check]
37                    Unknown                              [check]
38            Kaempferol-O-rutinoside                      [check]
39        Oxo-dihydroxy-octadecenoic acid        [check]   [check]
40          Trihydroxy-octadecenoic acid         [check]   [check]
41        Quercetin-rhamnoside-rhamnoside-
                      hexoside
42        Quercetin-rhamnoside-rhamnoside-                 [check]
                      hexoside
43      Kaempferol-rhamnose-hexose-rhamnose                [check]
44             Kaempferol derivative                       [check]

Table 2: Quantification of compounds in extracts of Q. coccifera.
Results are expressed in mg/g DE.

No.            Assigned               Acorns              Leaves
            identification

Gallic acid derivatives

5         Digalloyl-hexoside     2.6 [+ or -] 0.1
7         Digalloyl-hexoside     2.1 [+ or -] 0.1
8+9      Galloyl derivatives     8.4 [+ or -] 0.6
11          Methyl gallate        21 [+ or -] 1
12+13    Galloyl derivatives     5.7 [+ or -] 0.3
16       Trigalloyl-hexoside     1.9 [+ or -] 0.1
18       Trigalloyl-hexoside     2.4 [+ or -] 0.2
19       Trigalloyl-hexoside     3.9 [+ or -] 0.2
20          Trigallic acid       1.3 [+ or -] 0.1
22      Galloyl methyl gallate    22 [+ or -] 1
25      Tetragalloyl-hexoside     26 [+ or -] 1
27      Pentagalloyl-hexoside                         57 [+ or -] 2
28+29    Galloyl derivatives      52 [+ or -] 2
30      Gallic acid derivative                       1.7 [+ or -] 0.1
31      Pentagalloyl-hexoside     22 [+ or -] 0.2
34         Digalloyl methyl      3.5 [+ or -] 0.2
               gallate
Total                             175 [+ or -] 3       59 [+ or -] 2

Flavonoids
10             Catechin                             16.4 [+ or -] 0.8
23       Quercetin hexoside-                         1.4 [+ or -] 0.1
               gallate
26         (epi)catechin-O-                         1.06 [+ or -] 0.05
               gallate
38          Kaempferol-O-                           0.10 [+ or -] 0.04
              rutinoside
41       Quercetin-rhamnoside                       0.08 [+ or -] 0.01
         -rhamnoside-hexoside
42       Quercetin-rhamnoside                       0.05 [+ or -] 0.01
         -rhamnoside-hexoside
43       Kaempferol-rhamnose                        0.16 [+ or -] 0.01
           -hexose-rhamnose
Total                                                 19 [+ or -] 2
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Title Annotation:Research Article
Author:Molina-Garcia, L.; Martinez-Exposito, R.; Fernandez-de Cordova, M.L.; Llorent-Martinez, E.J.
Publication:Journal of Chemistry
Date:Jan 1, 2018
Words:6492
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