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Detection of transmissible spongiform encephalopathies using proteins found in serum and cerebrospinal fluid. (Medical Science and Health Poster Session 09:00 AM-10:00 AM).

BOARD 11

Transmissible spongiform encephalopathies (TSEs) are a group of fatal neurologic diseases affecting both animals and humans causing a "sponge-like" degeneration of brain tissue. These diseases are associated with the accumulation of a prion protein in nerve cells that lead to death of the host. The demonstration that prions are responsible for BSE and can spread to humans has lead to urgency to develop early detection methods. From previous research, it has been shown that alterations in levels several biochemical markers that indicate neurological damage of TSEs can be detected in cerebrospinal fluid and serum of infected animals. Specifically, two proteins, 14-3-3 (gamma isoform) and S-100, have been shown to be elevated in TSEs, such as Cruetzfelt-Jakob disease. The goal of this research is to develop a panel of rapid and unambiguous diagnostic tests to detect TSEs using sheep scrapie as a model. ELISA and Western Blot based tests are under development to examine the presence of 14-3-3 and S-100 in matched samples of plasma and CSF of sheep. Positive samples were histologically confirmed cases of scrapie received from the Ohio Dept of Agriculture. Negative samples were collected from apparently normal animals from scrapie infected and scrapie free flocks at the Ohio State University in Columbus and Wooster, Ohio. The last goal of the project is to convert the double antibody sandwich ELISA systems to a standard, simple to use, lateral flow device to identify both 14-3-3 and S-100 in samples of serum and CSF of sheep potentially infected with scrapie.

KATHARINE M. RINGER KRINGER@WOOSTER.EDU, (DEAN FRAGA DFRAGA@WOOSTER.EDU, SRINAND SREEVATSAN SREEVATSAN.1@OSU.EDU), COLLEGE OF WOOSTER, 1189 BEALL AVE, WOOSTER OH 44691
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Author:Ringer, Katharine M.; Fraga, Dean; Sreevatsan, Srinand
Publication:The Ohio Journal of Science
Article Type:Abstract
Date:Mar 1, 2003
Words:278
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