Detecting formaldehyde and dimethylamine in fish
Investigators have developed a fast, reliable test for TMOase, based on the reaction of the generated formaldehyde with nicotinamide dinucleotide, the product of which is measured spectrophotometrically, or by using a microplate reader. A simple low-cost test system has also been developed in which formaldehyde levels are determined with the use of a test strip.
Very high TMOase activity was found in the kidney, spleen and stomach wall of cod, saithe and whiting. These tissues were also used to determine the intracellular localization of TMOase activity. It was highest in the lysosomal and mitochondrial fractions. These data will help determine the quality and shelflife of fish and will be of major use to fish processors and distributors.
Further information. H. Rehbein, Federal Research Centre for Fisheries, Institute of Biochemistry and Technology, Palmaille 9, D-22767 Hamburg, Germany; phone: +49-40-38905-167; Fax: +49-40-38905-262.
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|Publication:||Emerging Food R&D Report|
|Date:||Apr 1, 1998|
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