Degradation Kinetics, In Vitro Dissolution Studies, and Quantification of Praziquantel, Anchored in Emission Intensity by Spectrofluorimetry/Degradasyon Kinetigi, In Vitro Cozunme Calismalari ve Baglanmis Prazikuantelin Emisyon Yogunluklu Spektrofluorimetri ile Miktar Tayini.
Chemically, praziquantel is 2-cyclohexanecarbonyl-1H, 2H,3H,4H,6H,7H,11bH-piperazino [2,1-a] isoquinolin-4-one (Figure 1). It is within the category of anthelmintics, used for the treatment of schistosome and many cestode infestations. Schistosomiasis is a parasitic disease caused by worms that penetrate the skin of people through water. Praziquantel is the only drug for treatment of schistosomiasis and it is effective and safe. Work is ongoing to expand the treatment with praziquantel to young children as well. (1,2)
An extended literature survey revealed that few analytical methods [using high-performance liquid chromatography (HPLC) and HPLC-mass spectrometry] have been reported for estimation of praziquantel. (3-7) To date, one spectrofluorimetric method has been reported for praziquantel quantification in blood plasma and urine. However, the method was complicated because of drug chemical derivatization with dansyl chloride, which involved various solvents with many extraction procedures. Keeping the above points in mind, our objective in this work was to develop a simple method for praziquantel quantification in pharmaceutical dosage forms in the presence of degradation products and in vitro dissolution samples. Spectrofluorimetry has implicit a foremost role in drug analysis because of its greater sensitivity and specificity than absorption spectrophotometry. Fluorimetry involves the estimation of drug solution at two wavelengths, excitation and emission in fluorescence; an emission spectrum is acquired by scanning the emission monochromator at determined emission wavelengths ([[lambda].sub.em]), at a particular excitation wavelength ([[lambda].sub.ex]). (8-13)
Nowadays, dissolution tests are routinely used in the pharmaceutical industry in a wide variety of applications, to identify which sort of formulation will produce the best results in the clinic, to release products into the market, and to verify batch-to-batch reproducibility. (14,15) International Conference on Harmonization (ICH) Q1A guidelines suggested procedures to carry out stress degradation studies to ensure the high quality, safety, and efficacy of the pharmaceutical product during its storage. Forced degradation (FD) study/stress testing is a process in which the natural degradation rate of a pharmaceutical product is increased by the application of an additional stress. It is an important part of the drug development process as it provides knowledge about the degradation chemistry of drug compounds to develop stability-indicating analytical methods and characterization of degradation products. (16)
Taking all these points into consideration, the objective of the present work was to develop and validate a simple spectrofluorimetric method for quantification of praziquantel in pharmaceutical dosage forms. The proposed method was applied to study the in vitro dissolution profiles of praziquantel and further extended to stress degradation studies of praziquantel and degradation kinetics were also calculated.
MATERIALS AND METHODS
Chemicals and reagents
All chemicals and reagents were of analytical grade. Pure praziquantel (99.50%) was obtained as gift samples from Taj Pharmaceutical Ltd. (Mumbai, India). Tablets (Zenticide) were obtained from local pharmacies. Glacial acetic acid (99.8-100.5%), ethanol, methanol, hydrochloric acid (36.46%), ortho-phosphoric acid, sodium hydroxide, hydrogen peroxide, dimethyl sulphoxide (DMSO), dimethyl formamide (DMF), acetonitrile, and potassium di-hydrogen phosphate were purchased from SD Fine Chemicals Ltd. (Mumbai, India). Series of buffer solutions of acetate buffers (pH 3.7-4.7) and phosphate buffers (pH 5.8-7.8) were prepared as per the Indian Pharmacopeia. (17)
A Shimadzu (Japan) RF-5301 PC spectrofluorophotometer was used for measurement of the fluorescence intensity of the selected drug. It was equipped with a 150-W Xenon arc lamp, a 1-cm non-fluorescent quartz cell was used, and it was connected to RFPC software. The instrument was operated at both low and high sensitivity with excitation and emission slit width set at 5 nm. An analytical balance (Shimadzu AUX 220, Japan), dissolution apparatus (Electro Lab, TDT-08L, India), pH meter (Elico), hot air oven, and ultraviolet (UV)-cabinet (Bio-Technics, India) were also used.
Preparation of standard stock solutions
Pure praziquantel drug of 10.0 mg was weighed and placed into a 10.0-mL volumetric flask and dissolved in methanol. The flask was shaken and volume was made up to the mark with distilled water. Then 0.1 mL of prepared solution was diluted to 10.0 mL with distilled water to attain praziquantel end concentration of 10.0 [micro]g/mL.
Construction of the calibration graph
The standard stock solution of praziquantel (100 [micro]g/mL) was used to prepare a set of diluted standard solutions of various concentrations (1-20 [micro]g/mL) prepared by pipetting appropriate volumes (0.1, 0.5, 1, 1.5, 2 mL) of stock solution into 10.0-mL volumetric flasks and adjusting the volume to the mark with distilled water. These solutions were scanned in a spectrofluorimeter at [[lambda].sub.ex] of 263 nm.
Determination of praziquantel in tablet dosage form (assay)
Twenty tablets of marketed formulation (Zenticide[R]), each containing 600 mg of praziquantel, were taken and accurately weighed. Average weight was determined and they were crushed into fine powder. An accurately weighed profusion of powder equivalent to 10.0 mg of praziquantel was transferred to a 10.0 mL volumetric flask. Methanol was added to this volumetric flask and it was sonicated for 15 min. The flask was shaken and volume was made up to the mark with water. The above solution was filtered through Whatman filter paper (no. 41). From the filtrate, a final concentration of solution was prepared for the estimation of drug content by the proposed method.
Dissolution testing of the praziquantel tablet formulation (Zenticide[R]) was carried out in 0.1 M HCl (900 mL) containing 2 mg of sodium lauryl sulfate according to the Food and Drug Administration (FDA) dissolution database, using a paddle apparatus (type 2) at 50 rpm and 37[+ or -]0.5[degrees]C for 60 min. Sampling aliquots of 5.0 mL were withdrawn at 10, 20, 30, 40, 50, and 60 min and reconstituted with an equal volume of the fresh medium to maintain the sink conditions. At the end of each test time, sample aliquots were filtered, diluted with water, and quantified. Samples withdrawn were evaluated with the regression equation of the proposed analytical technique for quantification of the dissolved drug followed by a plot against time.
Forced degradation studies
FD studies were carried out by exposing the sample solution to stress conditions like acidic, alkaline, thermal, oxidative, and UV effects.
Sample solution (10 [micro]g/mL) was prepared by adding 1.0 mL of stock solution and 2.0 mL of 5 M HCl to a 10.0 mL volumetric flask. Then the volumetric flask was kept under 60[degrees]C reflux conditions for 5 h and neutralized with 5 M NaOH; then the volume was made up to the mark with distilled water. Similarly, alkaline degradation (5 M NaOH), oxidative degradation (1% [H.sub.2][O.sub.2]), and thermal degradation (heated at 105[degrees]C) were performed. UV degradation was performed with 10 mg of praziquantel placed in a UV cabinet at short wavelength (254 nm), then subjected to the proposed sample procedure, and fluorescence intensity was measured every 1 h up to 24 h.
RESULTS AND DISCUSSION
Selection of wavelength
To attain a sensitive and specific spectrofluorimetric method for the quantification of praziquantel, several solvent systems were investigated such as methanol, DMSO, ethanol, DMF, and glacial acetic acid. Fluorescence intensity was higher in methanol:water (1:9) than in the other solvent systems (Figure 2) and exhibited native fluorescence at emission wavelength 286 nm after excitation at 263 nm in the presence of a polycyclic aromatic system like pyrazino and isoquinolin rings with more Jb electrons (Figure 3). Hence methanol:water (1:9) was selected as the solvent for quantification, FD studies, and in vitro dissolution studies of praziquantel.
Analytical method validation
Linearity and range
Linearity was verified qualitatively by running the plot; at several concentrations, the plot showed that amplitudes gradually changed (increased) at 286 nm (Figure 4). This was clear evidence of the suitability of the method for analysis. The linearity was assessed by the regression equation of the calibration curve; furthermore, the data and regression equation of calibration curves shown in Table 1 and Figure 5 denote that responses for praziquantel at 286 nm were linear in the concentration range of 1-20 [micro]g/mL, with a correlation coefficient ([R.sup.2]) value of 0.996.
The fluorescence spectrum attained from the commercial formulation solution was compared with the spectrum of standard solution (praziquantel). Both the commercial formulation spectrum and the standard solutions spectrum were superimposed; they denoted no interference from excipients with response of praziquantel at analytical wavelength 286 nm. Hence the method was found to be specific and selective.
Accuracy (recovery studies)
Accuracy was assessed by standard addition method at three distinct levels (80%, 100%, and 120%) of standards coalesced to commercial tablets in triplicate and the mean of percentage recoveries and % relative standard deviation (RSD) values were computed and are given in Table 2. The % RSD values at relevant concentration level was 2 and % recoveries of praziquantel were assessed to be in the range of 99-101, thus suggesting the accuracy of the method. (18)
Intra-day and inter-day precision harmony was appraised as per ICH guidelines, in which samples containing praziquantel (5/10 and 15 [micro]g/mL) were analyzed six times on the same day (intra-day precision) and for three consecutive days (inter-day precision); then % RSD was calculated. Results of intra-day and inter-day precision data are given in Table 3, which revealed that there was no significant distinction between the % RSD values of intra-day and inter-day analysis, which indicates that the proposed method is highly precise.
Limit of detection and limit of quantitation
The limit of detection and limit of quantitation were discretely appraised based on the standard calibration curve, and the results are presented in Table 1.
The proposed method was employed for the assay of commercial tablets (Zenticide[R]) containing praziquantel (600 mg). The results were correlated with consequent labeled amounts and are shown in Table 4. The best peaks of the assay in tablets were found to be 100.1 (% RSD<2), which indicates the accuracy of the proposed method.
In vitro dissolution testing
Dissolution studies on praziquantel tablets were performed with the FDA dissolution test medium as United States Pharmacopeia dissolution apparatus type 2 containing 900 mL of 0.1 M HCl with 2.0 mg of sodium lauryl sulfate as dissolution medium at a paddle speed of 50 rpm at temperature 37[+ or -]0.5[degrees]C. These results and profiles are given in Figure 6, which revealed that 95% of praziquantel was dissolved from the tablet dosage form.
Forced degradation studies
The stability of praziquantel was studied to elucidate its inherent stability characteristics in terms of fluorescence intensity in acid/alkali hydrolysis, oxidative, thermal, and UV light conditions, Anon constructed the plot between % drug degraded vs time interval. Degradation was observed in 5 M HCl (81% up to 60 min) and 5 M NaOH (1.0% up to 60 min) and 1% [H.sub.2][O.sub.2] (84% up to 60 min) but there was no evidence of degradation under UV light or thermal conditions (data not shown). For acidic and oxidative degradation it was observed that relative fluorescence intensity decreased gradually with increased heating time (Figure 7). Constructed the plot between logarithmic concentration against time shown in Figures 8, 9, calculated the half-life and first order rate constants for acid and oxidative degradation.
The proposed spectrofluorimetric method has the advantages of being economical, simple, sensitive, specific, and extraction free for quantification of praziquantel in tablets and in vitro dissolution samples. Furthermore, the present work examined the degradation kinetics of praziquantel under stress conditions as per ICH guidelines. Fortunately, considerable degradation was observed and the rate constant and half-life under acidic and oxidative stress conditions were calculated. The results of the proposed spectrofluorimetric method were clear evidence that it can be employed fruitfully for quantification of praziquantel in tablet dosage form, in in vitro dissolution samples without any interference, and in degradation studies.
The authors are thankful to the management and Principal Dr CVS Subrahmanyam, of Gokaraju Rangaraju College of Pharmacy for providing facilities for this research work.
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[iD] Panikumar D. ANUMOLU, [iD] Sunitha GURRALA*, [iD] Ceema MATHEW, [iD] Vasavi PANCHAKATLA, [iD] Veda MADDALA
Osmania University, Gokaraju Rangaraju College of Pharmacy, Department of Pharmaceutical Analysis, Hyderabad, India
Conflict of Interest: No conflict of interest was declared by the authors.
Table 1. Optimized parameters for the proposed method Parameter Value Excitation wavelength (nm) 263 Emission wavelength (nm) 286 Linearity range ([micro]g/mL) 1-20 Limit of detection ([micro]g/mL) 0.27 Limit of quantification ([micro]g/mL) 0.81 Correlation coefficient ([r.sup.2]) 0.996 Slope (m) 0.574 Intercept (c) 0.409 Regression equation Y=0.574x+0.409 Table 2. Accuracy (% recovery) of the proposed method Formulation Recovery Theoretical Concentration level (%) content found ([micro]g/mL) ([micro]g/mL) (mean [+ or -] SD) 80 18 17.93[+ or -]0.072 Zenticide[R] 100 20 20.12[+ or -]0.164 120 22 22.45[+ or -]0.390 Formulation % Amount % RSD recovered (mean [+ or -] SD) 99.6[+ or -]0.677 0.679 Zenticide[R] 100.6[+ or -]1.361 1.352 102.0[+ or -]0.264 0.258 SD: Standard deviation, RSD: Relative standard deviation Table 3. Precision data of the proposed analytical method Theoretical Intra-day Inter-day concentration Concentration Concentration ([micro]g/mL) (mean (a) [+ or -] SD) % RSD (mean (b) [+ or -] SD) 5 4.9[+ or -]0.018 0.36 5.0[+ or -]0.016 10 9.8[+ or -]0.0169 0.17 9.9[+ or -]0.024 15 15.1[+ or -]0.023 0.15 14.9[+ or -]0.035 Theoretical concentration ([micro]g/mL) % RSD 5 0.32 10 0.24 15 0.23 (a) : Mean values of six different standards for each concentration, (b) : Inter-day reproducibility was quantified from six different standards of each concentration for three consecutive days, SD: Standard deviation, RSD: Relative standard deviation Table 4. Assay results of marketed tables of praziquantel Praziquantel Formulation Label claim Amount found (mg) % Assay % RSD (mg) (mean [+ or -] SD) (n=3) Zenticide 600 600.8[+ or -]0.08 100.1% 0.013 SD: Standard deviation, RSD: Relative standard deviation Table 5. Results of the degradation study of praziquantel under acidic and oxidative conditions on the kinetic parameters of praziquantel Degradation condition Reaction rate constant Half-life time (K [min.sup.-1]) ([t.sub.1/2] min) Acidic degradation (5 N 0.027 25.6 HCl, 60[degrees]C) Oxidative degradation 0.030 23 (1% [H.sub.2][O.sub.2])
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|Title Annotation:||ORIGINAL ARTICLE|
|Author:||Anumolu, Panikumar D.; Gurrala, Sunitha; Mathew, Ceema; panchakatla, Vasav; Maddala, Veda|
|Publication:||Turkish Journal of Pharmaceutical Sciences|
|Date:||Mar 1, 2019|
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