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Cryptococcus gattii VGIIb-like variant in white-tailed deer, Nova Scotia, Canada.

To the Editor: Cryptococcus gattii is a fungal pathogen that is emerging in the Pacific Northwest of North America. In Nova Scotia, Canada, previously not recognized as a C. gattii-endemic area, a variant strain similar to VGIIb caused cryptococcosis with nasopulmonary, lymph node and central nervous system involvement in a free-ranging, yearling white-tailed deer (Odocoileus virginianus). The deer was found in the village of Greenwood (latitude 44.9717246; longitude -64.9341295) on July 14, 2014. The deer exhibited behavioral and neurologic abnormalities, including loss of fear of humans, ataxia, circling, high-stepping gait, torticollis, and a fixed stare. Additional clinical signs were ptyalism with frothing from the mouth and dyspnea with gurgling respiration. The animal was euthanized, and the head, lungs, heart, gastrointestinal tract, liver, and kidneys were submitted for pathologic examination.

Gross examination revealed multifocal, soft, round, expansile, pale tan masses of variable sizes that had replaced or effaced the normal architecture of the tracheobronchial lymph nodes and pulmonary parenchyma. The center of the largest lymph node mass was necrotic and filled with viscous yellow material. Similar yellow gelatinous material obliterated the right ethmoturbinates rostral to the cribriform plate. In the brain, cerebellar coning was prominent. Several small, pitted lesions with dark rims were noted in the neuropil of the thalami, superior colliculi, and hippocampus. Gross lesions were absent in the liver, kidney, and gastrointestinal tract (online Technical Appendix, http://wwwnc.cdc.gov/ EID/article/22/6/16-0081-Techapp1.pdf).

Microscopically, the nasal cavity, lung, tracheobronchial lymph node, and brain lesions were similar, consisting of variably sized, cystic spaces supported by various thicknesses of well-differentiated fibrovascular septa or remaining normal parenchyma. The cystic spaces, immediately adjacent tissues, meninges, and ependyma contained variable numbers of yeast associated with a granulomatous inflammatory response or a pleocellular population of lymphocytes, plasma cells, macrophages, and neutrophils (Figure). The yeast were round to oval, 15-33 [micro]m in total diameter, with poorly staining central portions (5-12 pm in diameter) surrounded by a pale acidophilic or basophilic capsule (5-21 [micro]m thick), which stained positively with a mucicarmine stain. Some yeast were dematiaceous, and Fontana-Masson staining was consistent with presence of melanin. Very rarely, narrow-based budding was observed in the yeast. All aforementioned morphologic characteristics, staining affinities, and lesion distributions are consistent with an infection with fungi in the genus Cryptococcus (1,2).

One species of Cryptococcus was isolated from a tracheobronchial lymph node aspirate. Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry fingerprinting of the isolate (Biotyper RTC software; Bruker Daltonics Ltd, Bremen, Germany) yielded 8 diagnostic signals consistent with C. gattii VGIIb and VGIIc; therefore, the isolate was further classified by multilocus sequence typing based on 7 genetic loci, following the International Society for Human and Animal Mycology consensus multilocus sequence typing scheme for the C. neoformans/C. gattii species complex (3). Further discrimination based on allele congruence with established C. gattii VGII genotypes (4) classified the isolate as being most similar to genotype C. gattii VGIIb (CAP59 allele no. 2, GPD1 allele no. 6, LAC1 allele no. 4, PLB1 allele no. 2, URA5 allele no. 2, IGS1 allele no. 10). However, because of a slight difference in the SOD1 allele (99.5% similarity with allele no. 15), this strain is considered to be a unique variant strain, most similar to that of the VGIIb genotype. Whole-genotyping studies have provided evidence of multiple distinct introductions of the VGIIb genotype to North America (5). Because of the observed difference in the SOD1 allele, the VGIIb-like variant strain may represent a fourth introduction or a different VGII genotype altogether.

The white-tailed deer represents a new host species for C. gattii in North America. Because white-tailed deer are nonmigratory, generally exhibiting only minor seasonal movements (6), this infection was considered to be autochthonous, indicating endemicity of the C. gattii VGIIb-like variant in Nova Scotia and highlighting the value of nonmigratory animals as sentinels for emerging diseases (7). Incidence for this disease is highest in the Pacific Northwest, where the primary agents are C. gattii VGII genotypes (2,4). A pertinent literature review and consultation with regional public and veterinary health authorities determined that Quebec was the most eastern province in Canada where crytococcosis associated with C. gattii VGII has caused clinical disease that was not potentially travel related in humans (Phillippe Dufresne, pers. comm.). In eastern North America, the C. gattii VGIIb genotype is reported to have caused disseminated cryptococcosis in a human in Florida, USA (8,9). Because C. gattii is potentially pervasive in the environment, the Nova Scotia Department of Health has alerted provincial infectious disease specialists and the provincial public health laboratory to ensure availability of the diagnostic capacity to test for the fungus.

The C. gattii VGIIb genotype causes substantial, life-threatening disease in otherwise healthy hosts (2), and a unique VGIIb-like variant is endemic to Atlantic Canada. Therefore, continued surveillance by physicians and veterinarians in the region is warranted.

Acknowledgments

We thank Beatrice Despres for her technical support in the operation and maintenance of matrix-assisted laser desorption/ ionization time-of-flight mass spectrometry for sample analysis. We also thank Bob Petri and Donald Sam for their ongoing support of the scanning wildlife health surveillance program in Atlantic Canada and encouragement of Nova Scotia Department of Natural Resources field staff to take the time and make the effort to submit diagnostic specimens from the white-tailed deer, enabling a confirmatory diagnosis for this index case of cryptococcosis in Atlantic Canada.

The cost of the diagnostic testing for this case was covered by the scanning wildlife health surveillance program of the Canadian Wildlife Health Cooperative.

References

(1.) Lester SJ, Malik R, Bartlett KH, Duncan CG. Cryptococcosis: update and emergence of Cryptococcus gattii. Vet Clin Pathol. 2011; 40:4-17. http://dx.doi.org/10.1111/j.1939-165X.2010.00281.x

(2.) Espinel-Ingroff A, Kidd SE. Current trends in the prevalence of Cryptococcus gattii in the United States and Canada. Infect Drug Resist. 2015; 8:89-97. http://dx.doi.org/10.2147/IDR.S57686

(3.) Meyer W, Aanensen DM, Boekhout T, Cogliati M, Diaz MR, Esposto MC, et al. Consensus multi-locus sequence typing scheme for Cryptococcus neoformans and Cryptococcus gattii. J Med Mycol. 2009; 47:561-70. http://dx.doi.org/10.1080/ 13693780902953886

(4.) Byrnes EJ, Li W, Lewit Y, Ma H, Voelz K, Ren P, et al. Emergence and pathogenicity of highly virulent Cryptococcus gattii genotypes in the Northwest United States. PLoS Pathog. 2010; 6:e1000850. http://dx.doi.org/10.1371/journal.ppat.1000850

(5.) Engelthaler DM, Hicks ND, Gillece JD, Roe CC, Schupp JM, Driebe EM, et al. Cryptococcus gattii in North America Pacific Northwest: whole-population genome analysis provides insights into the species evolution and dispersal. mBiol. 2014; 5:e0146414. http://dx.doi.org/10.1128/mBio.01464-14

(6.) Marchinton Rl, Hirth DH. Chapter 6: Behavior. In: Halls LK, editor. White-tailed deer: ecology and management. Harrisburg (PA): Stackpole Books; 1984. p. 129-68.

(7.) Duncan C, Schwantje H, Stephen C, Campbell J, Bartlett K. Cryptococcus gattii in wildlife of Vancouver Island, British Columbia, Canada. J Wildl Dis. 2006; 42:175-8. http://dx.doi.org/ 10.7589/0090-3558-42.1.175

(8.) Kunadharaju R, Choe U, Harris JR, Lockhart SR, Greene JN. Cryptococcus gattii, Florida, USA, 2011. Emerg Infect Dis. 2013; 19:519-21. http://dx.doi.org/10.3201/eid1903.121399

(9.) Lockhart SR, Iqbal N, Harris JR, Grossman NT, DeBess E, Wohrle R, et al. Cryptococcus gattii in the United States: genotypic diversity of human and veterinary isolates. PLoS ONE. 2013; 8:e74737 http://dx.doi.org/10.1371/journal.pone.0074737.

Address for correspondence: David P. Overy, Department of Pathology and Microbiology, Atlantic Veterinary College, University of Prince Edward Island, 550 University of Prince Edward Island, Charlottetown, PEI, Canada; email: dovery@upei.ca

David P. Overy, [1] Scott McBurney, [1] Anne Muckle, Lorraine Lund, P. Jeffery Lewis, Robert Strang

[1] These authors contributed equally to this article.

Author affiliations: Atlantic Veterinary College, University of Prince Edward Island, Charlottetown, Prince Edward Island, Canada (D.P. Overy, S. McBurney, A. Muckle, L. Lund, PJ. Lewis); Department of Health and Wellness, Halifax, Nova Scotia, Canada (R. Strang)

DOI: http://dx.doi.org/10.3201/eid2206.160081
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Title Annotation:LETTERS
Author:Overy, David P.; McBurney, Scott; Muckle, Anne; Lund, Lorraine; Lewis, P. Jeffery; Strang, Robert
Publication:Emerging Infectious Diseases
Article Type:Report
Geographic Code:1CNOV
Date:Jun 1, 2016
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