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Creating a procedural control for the detection of methicillin--resistance in S. aureus. (Senior Division).

Antibiotic resistance in microbes, specifically resistance of Staphylococcus aureus to methicillin, is an increasing problem in hospitals. For example, the prevalence of methicillin resistance in S. aureus infections has increased from 2% in 1970 to roughly 50% today. Critical care patients are at the most risk for contracting staph that is resistant to methicillin, called MRSA. Therefore, it is important to have a test to monitor the presence of MRSA in the hospital setting. At Thermo Biostar, a test has been developed to measure the presence of a gene, mec A, responsible for methicillin in Staphylococcus aureus. The test is highly specific for measuring MRSA. Also the test is rapid and provides a visual result; instrumentation is not needed. This experiment consists of creating a positive control spot for the MRSA detection assay, this is to make sure that when testing for this particular strain of bacteria, the test is performed accurately. Chips of modified silicon, coated with two different DNA capture sequences were created. One DNA sequence is used to immobilize the mec A gene which is simultaneously hybridizing to detect DNA sequences in solution. The other captures the DNA sequence which contains part of the mec A gene that is recognized by the detector DNA sequence, thereby serving as a positive control for hybridization.

Alex Grimes, New Vista High School.
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Author:Grimes, Alex
Publication:Journal of the Colorado-Wyoming Academy of Science
Article Type:Brief Article
Geographic Code:1USA
Date:Apr 1, 2002
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