Printer Friendly

Control of synaptic function by endocannabinoid-mediated retrograde signaling.

1. Introduction

Marijuana, a derivative of the plant Cannabis sativa, has been used for recreational and therapeutic purposes for a thousands of years. Marijuana inhalation causes variety of psychomotor effects including euphoria and relaxation, feelings of well-being, grandiosity, altered perception of passage of time, visual distorsions, drowsiness, diminished coordination and memory impairment. Marijuana can also increase appetite, attenuate nausea, and relieve chronic pain, which led to the use of cannabinoids for therapeutic purposes. (1)), (2)) However, how marijuana modifies various brain functions had been a longstanding mystery. The first breakthrough of cannabinoid research is the identification of " [[DELTA].sup.9]-tetrahydrocannabinol ("[[DELTA].sup.9]-THC) as the main psychoactive component of marijuana in 1964. (3)) The second breakthrough is the identification of receptors for [[DELTA].sup.9]-THC. The first receptor, termed cannabinoid C[B.sub.1] receptor (C[B.sub.1]R), was found in 1990, (4)) and the second one, termed cannabinoid C[B.sub.2] receptor (C[B.sub.2]R), was reported in 1993. (5)) These are seven-transmembrane receptors coupled to [G.sub.i]/o protein and display distinct expression patterns in animal's body. C[B.sub.1]Rs are richly expressed in the brain, whereas CB2Rs are mainly expressed in the immune system of the periphery. The third breakthrough is the identification of endogenous ligands for cannabinoid receptors (endocannabinoid). The first endocannabinoid, N-arachidonoylethanolamine, was found in 1992 and termed "anandamide" after the Sanscrit word that means "bliss". (6)) In 1995, two groups identified another endocannabinoid, 2-arachidonoylglycerol (2-AG). (7)), (8)) Anandamide and 2-AG have been regarded as two major endocannabinoids in animal's tissues.

The fourth breakthrough, which is functionally most important, is the discovery that endocannabinoids mediate retrograde signaling at synapses. In 2001, my group and two other laboratories independently reported that depolarization of postsynaptic neurons causes endocannabinoid-mediated transient suppression of transmitter release from presynaptic terminals in the hippocampus and cerebellum. (9)-11)) It is now widely accepted that endocannabinoids are released from postsynaptic neurons upon postsynaptic depolarization and/or receptor activation, and act on presynaptic C[B.sub.1]R to induce transient suppression of transmitter release (endocannabinoid-mediated short-term depression; eCB-STD) (for review, see refs. 12-15). Moreover, endocannabinoid-mediated long-term depression (eCB-LTD) was reported in 2002 in the striatum (16)) and nucleus accumbens. (17) Later studies have confirmed that eCB-STD and eCB-LTD can be induced at variety of synapses throughout the central and peripheral nervous systems (for review, see refs. 12-15). In support of electrophysiological data, immunohistochemical studies demonstrate that the molecules for retrograde endocannabinoid signaling are arranged around synapses in functionally relevant manners. (18)) (-23)) These endocannabinoid signaling molecules are richly expressed in various brain areas including the hippocampus, cerebral cortex, amygdale, dorsal and ventral striatum, hypothalamus, cerebellum, and spinal cord. (13)), (24)) These brain regions are considered to be responsible for the neural functions that have been shown to depend on endocannabinoid signaling. Behavioral studies with pharmacological and genetic manipulation of retrograde endocannabinoid signaling have demonstrated that it is involved in various aspects of neural functions, including learning and memory, mood and anxiety, drug addiction, feeding behavior, motor learning and analgesia (for review, see refs. 13, 25).

Recent studies have revealed that the endocan-nabinoid system has diverse roles in the modulation of neural circuit function over eCB-STD/LTD. Moreover, endocannabinoid signaling itself has been shown to undergo plastic changes in response to various factors. This review provides an up to date understanding of how endocannabinoid signaling modulates synaptic transmission and neural circuit functions. Space limitation allows me to include a limited number of references. For more information about original papers, several excellent reviews are available. (12)-15)-26)-30))

2. Molecules responsible for endocannabinoid-mediated synaptic modulation C[B.sub.1]R

The C[B.sub.1]R, the first identified receptor for [[DELTA].sup.9]-THC, is expressed widely throughout the brain but its distribution is heterogeneous at the cellular level. (19)) (-23)) For example, in the cerebral cortex, hippocampus and amygdala, C[B.sub.1]R is highly expressed in a subset of GABAergic inhibitory interneurons that express an intestinal peptide hormone, cholecystokinin (CCK), but almost absent in another class of inhibitory neurons that possess a calcium-binding protein, parvalbumin. (31)) In contrast, C[B.sub.1]R is weakly expressed in most excitatory neurons. (18)-19)) The C[B.sub.1]R is preferentially targeted to presynaptic terminals and axons. The expression level of the C[B.sub.1]R at presynaptic terminals varies greatly depending on brain regions and synapse types. For example, in the cerebellum, the [CB.sub.1]R is highly expressed in parallel fibers (i.e., the axons of granule cells) and their presynaptic terminals, inhibitory presynaptic terminals of interneurons in the molecular layer, but is absent in axons of Purkinje cells. (22)) In the striatum, the C[B.sub.1]R is highly expressed in inhibitory presynaptic terminals of medium spiny neurons and parvalbumin-positive interneurons, whereas its expression is low at excitatory presynaptic terminals from the cortex and is absent at excitatory terminals from the thalamus. (20))

Activation of C[B.sub.1]R triggers multiple signal transduction pathways mainly through the [G.sub.i]/o family of heterotrimeric G proteins, including decreases in cyclic AMP (cAMP) level and protein kinase A (PKA) activity, inhibition of voltage-gated [Ca.sup.2+] channels, activation of several types of [K.sup.+] channels, and suppression of transmitter release. (32)) Presynaptic mechanisms underlying endocannabinoid-mediated suppression of transmitter release involve inhibition of [Ca.sup.2+] channels, activation of [K.sup.+] channels, and inhibition of some release mechanisms downstream of [Ca.sup.2+] influx. Relative contributions of these three mechanisms for suppression of transmitter release may be different at different synapses.

Anandamide. Anandamide is a partial agonist for C[B.sub.1]R, C[B.sub.2]R and a full agonist for the transient receptor potential vanilloid type 1 (TRPV1) receptor, (33)) a cation channel to which vanilloids such as capsaicin bind. Biochemical pathways for the synthesis of anandamide have not yet been fully understood. Early studies suggested that N-acyltransferase and N-arachidonoyl phosphatidylethanolamine-hydrolyzing phospholipase D (NAPE-PLD) play a major role for anandamide biosynthesis. Later studies using NAPE-PLD-knockout mice, however, revealed that anandamide can be produced in an NAPE-PLD-independent manner. (34) The major enzyme catalyzing anandamide degradation is fatty acid amide hydrolase (FAAH), which was purified and cloned in 1996. (35))

2-AG. 2-AG functions as a full agonist of C[B.sub.1]R and C[B.sub.2]R, and its content in the brain is much higher than that of anandamide. (36)) Biochemical studies have found several pathways for 2-AG biosynthesis. The main pathway is the formation from membrane phospholipids through the combined actions of phospholipase C (PLC) and diacylglycerol lipase (DGL) (Fig. 1). Two highly related DGLs were cloned and named DGL[alpha] and DGL[beta]. (37)) Detailed immunoelectron microscopic analyses demonstrate that [DGL.sub.[alpha]] is expressed postsynaptically, particularly concentrated at the spine head in hippocampal pyramidal cells (18)), (20)) or at spine neck in cerebellar Purkinje cells. (22))

2-AG is hydrolyzed into arachidonic acid and glycerol primarily by monoacylglycerol lipase (MGL), which was first cloned from mouse adipose tissue. (36)) A functional proteomic study has demonstrated that MGL accounts for ~85% of 2-AG hydrolysis, and that other enzymes, such as [alpha]/[beta] hydrolase domain 6 and 12 (ABHD6 and ABHD12), contributes to the hydrolysis of the remaining 15%. (38)) Since MGL and ABHD6 display different distribution patterns, the former being presynaptic and the latter being postsynaptic (Fig. 1), these two enzymes are considered to have non-overlapping functions. (39)) Interestingly, a neurodegenerative disease named PHARC (polyneuropathy, hearing loss, ataxia, retinitis pigmentosa, and cataract) is caused by mutations in the ABHD12 gene, indicating that ABHD12 plays important roles in the central and peripheral nervous systems and the eye. (40)) The apparent paradox of its minor contribution to 2-AG hydrolysis versus serious PHARC phenotype remains to be resolved. Besides hydrolysis, oxidation by cyclooxygenase (COX) is another pathway for 2-AG degradation. Among three forms of COX (COX-1, -2, -3), only COX-2 preferably recognizes 2-AG as an effective substrate. (41)) Since morphological data suggest that Cox-2 is expressed at postsynaptic sites, (42)) this enzyme may play complementary roles with MGL in 2-AG degradation.

3. Endocannabinoid-mediated short-term depression (eCB-STD)

Since the first reports on endocannabinoid as synaptic retrograde messengers in 2001, (9)-11)) numerous electrophysiological studies have been performed. It is now widely accepted that endocannabinoids function as retrograde messengers and contribute to short-term and long-term synaptic plasticity (for review, see refs. 12-15). The release of endocannabinoids from postsynaptic neurons is induced by various stimulation protocols that cause [Ca.sup.2+] elevation and/or activation of [G.sub.q/11]-coupled receptors.

After release from postsynaptic neurons, endocannabinoids activate presynaptic C[B.sub.1]R, and suppress transmitter release transiently (eCB-STD) or persistently (eCB-LTD). Various forms of eCB-STD/LTD have been reported to occur at inhibitory and excitatory synapses in various regions of the brain. In this section, I will discuss the molecular mechanisms that explain how endocannabinoids are produced and released in response to each stimulation protocol and induce eCB-STD.

STD depending on [Ca.sup.2+]-driven endocannabinoid release (CaER). Endocannabinoid release can be induced by a large postsynaptic [Ca.sup.2+] elevation alone, which has been termed [Ca.sup.2+]-driven endocannabinoid release (CaER). (13)), (15)) This form of eCB-STD includes DSI (depolarization-induced suppression of inhibition) (10))' (11)) (Fig. 2A), DSE (depolarization-induced suppression of excitation), (9)) and presynaptic suppression caused by [Ca.sup.2+] influx through NMDA-type glutamate receptors (NMDARs). (43))

DSI was reported in early 1990s first in the cerebellum and then in the hippocampus. In 1991, Llano et al. discovered that depolarization of cerebellar Purkinje cells induces transient suppression of inhibitory inputs to the depolarized cells. (44)) In 1992, Pitler and Alger reported the same phenomenon in hippocampal CA1 neurons and termed it "DSP'. (45)) Because DSI requires postsynaptic [Ca.sup.2+] elevation and causes suppression of transmitter release from presynaptic terminals, some mechanisms of "retrograde signaling" were thought to exist. Candidates of such retrograde messengers were sought, but it took 10 years to identify the mechanism that mediates DSI. In 2001, Ohno-Shosaku et al. and Wilson and Nicoll reported independently that endocannabinoids mediate retrograde signaling for DSI in the hippocampus ((10),11)) (see Fig. 2A). Since then, endocannabinoid-mediated DSI has been reported in various brain regions including the striatum, globus pallidus, substantia nigra, cerebral cortex, amygdala, and hypothalamus (for review, see refs. (13, 26, 46)).

At the same time of the discovery that endocannabinoids mediate DSI, Kreitzer and Regehr discovered that depolarization of cerebellar Purkinje cells induces transient suppression of excitatory transmission, (9)) which was termed DSE. They found that, similarly to DSI, DSE was induced by postsynaptic [Ca.sup.2+] elevation, expressed presynaptically, and mediated by retrograde endocannabinoid signaling. (9)) DSE has also been reported in many other brain regions including the hippocampus, cerebral cortex, hypothalamus, ventral tegmental area, and dorsal cochlear nucleus (for review, see refs. 13, 26, 46).

DSI and DSE are dependent on [Ca.sup.2+] influx to postsynaptic neurons through voltage-gated [Ca.sup.2+] channels. It was unclear whether the NMDA-type glutamate receptor (NMDAR), another major route of [Ca.sup.2+] influx into neurons, can contribute to endocannabinoid release. In 2007, Ohno-Shosaku et al. demonstrated that stimulation of NMDARs induces a transient suppression of inhibitory transmission in cultured hippocampal neurons, which requires postsynaptic [Ca.sup.2+] elevation and endocannabinoid signaling. (43)) Blockade of postsynaptic voltagegated [Ca.sup.2+] channels had no effect on NMDARdriven eCB-STD, indicating that [Ca.sup.2+] influx through NMDAR directly induces endocannabinoid release. (43))

A current model for the mechanisms of eCBSTD depending on CaER (DSI/DSE and NMDARdriven eCB-STD) is illustrated in Fig. 3(a). Activation of voltage-gated [Ca.sup.2+] channels or NMDARs causes a large [Ca.sup.2+] elevation in postsynaptic neurons, and produces 2-AG in a DGL,-dependent manner. 2-AG is then released from postsynaptic neurons, binds to presynaptic C[B.sub.1]R and suppresses transmitter release by inhibiting presynaptic voltagegated [Ca.sup.2+] channels. CaER requires elevation of intracellular [Ca.sup.2+] concentration to micromolar range, but the [Ca.sup.2+] level might be dependent on the duration of [Ca.sup.2+] elevation. Several lines of evidence strongly suggest that 2-AG rather than anandamide mediates eCB-STD depending on CaER. Inhibition of 2-AG hydrolyzing enzyme (MGL), but not anandamide hydrolyzing enzyme (FAAH) has been shown to prolong DSI/DSE. (47)) Most importantly, Tanimura et al. (2010) and Gao et al. (2010) demonstrated independently that DSI and/or DSE are totally absent in the hippocampus, striatum or cerebellum of mice lacking [DGL.sub.[alpha]]. (48)), (49)) Later, Yoshino et al. confirmed the lack of DSI in the prefrontal cortex of DGL[alpha] deficient mice. (50))

How [Ca.sup.2+] elevation in neurons leads to 2-AG synthesis and release in a DGL[alpha]-dependent manner remains unclear. A simple model is that [Ca.sup.2+] activates an unidentified enzyme that produces diacylglycerol which is then converted to 2-AG by DGL[alpha], as illustrated in Fig. 3(a). There is, however, a controversy as to this "on-demand 2-AG synthesis model" which I will discuss later.

STD depending on receptor-driven endocannabinoid release (RER). Soon after the reports on the roles of endocannabinoid signaling in DSI/DSE, Maejima et al. reported that activation of group I metabotropic glutamate receptor (mGluR) causes eCB-STD in cerebellar Purkinje cells in a [Ca.sup.2+]-independent manner. (51)) After this report, various Gq/11-coupled receptors including, [M.sub.1]/[M.sub.3] muscarinic, 5-HT2-type serotonin (5-HT2R), orexin and oxytocin receptors have been shown to be capable of driving eCB-STD (for review, see refs. 13-15). In these forms of eCB-STD, the endocannabinoid release is induced by strong activation of the receptors without need of postsynaptic [Ca.sup.2+] elevation, (51)) which is termed receptor-driven endocannabinoid release (RER). (13)), (15))

The mGluRs include eight subtypes (mGluR18), and are divided into three groups, namely group I (mGluR1 and 5), group II (mGluR2, 3) and group III (mGluR4, 6, 7 and 8). Group I mGluRs are coupled to [G.sub.q/11]-type of G proteins. In 2001, Maejima et al. reported that application of the group I mGluR agonist 3,5-dihydroxyphenylglycine (DHPG) caused a transient suppression of the excitatory transmission to Purkinje cells. (51)) This suppression was abolished when the G protein signaling in postsynaptic neurons is blocked by inclusion of GTP[gamma]S or GDP[beta]S in the recording pipette, indicating that some retrograde signaling from postsynaptic neurons to presynaptic terminals is required. This phenomenon occurred with a high concentration of the [Ca.sup.2+] chelator BAPTA in the recording pipette, indicating that group I mGluR activation can trigger eCB-STD in a [Ca.sup.2+]-independent manner. The mGluR-driven eCBSTD is now known to occur in various regions of the brain, including the hippocampus, striatum, nucleus accumbens, medial nucleus of the trapezoid body, and periaqueductal gray (for review, see refs. 13-15).

Subsequently, Kim et al. reported in hippocampal slices that a cholinergic agonist, carbachol, suppressed inhibitory synaptic transmission to CA1 pyramidal cells in a C[B.sub.1]R-dependent manner. (52) They showed pharmacologically that the receptors responsible for this effect were muscarinic acetylcholine receptors (mAChRs) rather than nicotinic receptors.52) Among the five subtypes of mAChRs ([M.sub.1]-[M.sub.5]), [M.sub.1], [M.sub.3] and [M.sub.5] receptors are coupled to [G.sub.q/11], whereas [M.sub.2] and [M.sup.4] receptors are coupled to [G.sub.i/o]. Fukudome et al. determined the subtypes of mAChRs involved in eCB-STD by using genetically engineered mice lacking each subtype of mAChRs. The data clearly demonstrate that [M.sub.1] and [M.sub.3] receptors are responsible for the induction of eCBSTD. (53)) The mAChR-driven eCB-STD is also found at hippocampal excitatory synapses (54)) and striatal inhibitory synapses. (55)

After the discoveries of mGluR- and mAChRdriven eCB-STDs, several groups demonstrated that other [G.sub.q/11]-coupled receptors also can drive eCBSTD. In the inferior olive in which [G.sub.q/11]-coupled [5HT.sub.2]Rs are highly expressed on neuronal dendrites and soma, serotonin receptor agonists suppressed excitatory synaptic transmission. This 5-HT2R-dependent component of suppression was abolished by blockade of C[B.sub.1]R, indicating that 5-HT2R drives eCB-STD. (56)) In addition, [G.sub.q/11]-coupled receptors for neuropeptide are also capable of driving endocannabinoid release. Suppressing effects of orexin-B on the excitatory transmission to serotonergic neurons in the dorsal raphe nucleus, (57)) and oxytocin on both the excitatory and inhibitory synaptic transmissions to magnocellular neurons in the supraoptic nucleus (58)) have been reported. These forms of synaptic suppression were C[B.sub.1]R-dependent, and therefore can be classified as receptor-driven eCB-STD.

Figure 3(b) illustrates a current model for the mechanisms underlying [G.sub.q/11]-coupled receptor-driven eCB-STD without need of [Ca.sup.2+] elevation. When postsynaptic [G.sub.q/11]-coupled receptors are stimulated, diacylglycerol is produced through PLC[beta], and converted to 2-AG by DGL[alpha]. 2-AG is then released from postsynaptic neurons, binds to presynaptic C[B.sub.1]R and suppresses transmitter release. PLC[beta] consists of 4 isoforms (PLC[beta]1-4) and the type of PLC[beta] is different in different brain areas. (59)) Hashimotodani et al. demonstrated that eCB-STD driven by mGluR or mAChR activation is absent in PLC[beta]1-deficient hippocampal neurons, (60)) while Maejima et al. found that mGluR-driven eCB-STD is absent in PLC[beta]4-deficient cerebellar Purkinje cells. (61)) These electro physiological data are consistent with those of in situ hybridization showing that PLC[beta]1 and PLC[beta]4 are the major PLCO isozymes in the hippocampus and the rostral portion of the cerebellum, respectively. (59)) Studies using DGL inhibitors (20)), (47)), (57)), (62)), (63)) and DGL[alpha]-KO mice (49)) support the involvement of DGL[alpha] in [G.sub.q/11]-coupled receptor-driven eCB-STD. The experimental results so far reported are largely consistent with this model. There is, however, also a controversy as to this "on-demand 2-AG synthesis model" and I will discuss this issue later.

STD depending on [Ca.sup.2+]-assisted RER. The third form of eCB-STD can be induced when small [Ca.sup.2+] elevation and weak [G.sub.q/11]-coupled-receptor activation, both of which are subthreshold for triggering endocannabinoid release, are applied simultaneously (52)), (55)), (60)), (61)), (64)) (-66)) (see Fig. 2B). This phenomenon results from the enhancement of RER by subthreshold [Ca.sup.2+] elevation. In cultured hippocampal neurons, Hashimotodani et al. monitored the degree of PLC[beta] activation by mAChR using the diacylglycerol-activated cation channel TRPC6 (transient receptor potential cation channel subfamily C member 6) as a biosensor for the PLC activity. (60)) They confirmed that the TRPC6-mediated inward current induced by muscarinic agonist was dependent on PLC[beta]1. Then they demonstrated that the current was highly sensitive to intracellular [Ca.sup.2+] concentration within a physiological range, and was greatly enhanced by a [Ca.sup.2+] elevation. (60)) Moreover, they confirmed that mGluR-driven and mAChR-driven eCB-STDs depended on intracellular [Ca.sup.2+] levels in the hippocampus. (60)) In the same year, Maejima et al. reported that mGluR-driven eCBSTD was dependent on intracellular [Ca.sup.2+] levels in cerebellar Purkinje cells. (61)) Figure 3(c) shows a current model of the mechanisms underlying the endocannabinoid release induced by combined subthreshold [Ca.sup.2+] elevation and receptor activation, which is termed [Ca.sup.2+]-assisted RER. (64)) When mild activation of postsynaptic [G.sub.q/11]-coupled receptors is combined with a small [Ca.sup.2+] elevation (submicromolar range), the endocannabinoid 2-AG is produced through PLCO-dependent pathway. Another possible candidate mediating [Ca.sup.2+]-assisted RER is PLC.. Lemtiri-Chlieh and Levine reported that BDNF suppresses inhibitory transmission in a C[B.sub.1]Rdependent manner in the neocortex, which is suppressed by inhibition of postsynaptic [Ca.sup.2+] rise. (67)) Although molecular mechanisms underlying this eCB-STD are not determined, a likely candidate is [Ca.sup.2+]-assisted RER involving PLC[gamma]. instead of PLC[beta].

Since weak depolarization of postsynaptic membrane and activation of postsynaptic [G.sub.q/11]-coupled receptors can be achieved by synaptic activity, [Ca.sup.2+]-assisted RER appears to be most relevant for synaptically-driven eCB-STD (see below) among CaER, RER and [Ca.sup.2+]-assisted RER. Another important feature of [Ca.sup.2+]-assisted RER is that this mechanism is suitable for detecting coincidence between the activity of presynaptic terminals and that of postsynaptic neurons. More specifically, PLCO functions as a coincidence detector of [G.sub.q/11]-coupled receptor activation and [Ca.sup.2+] elevation, leading to the production of 2-AG.60), (64)) In line with this notion, PLCO may play a crucial role as a coincident detector in timing-dependent eCB-LTD. (68))

Synaptically-driven eCB-STD. In several brain areas, synaptic activity has been demonstrated to induce eCB-STD, which is termed synapticallydriven eCB-STD. (13)), (66)) Repetitive stimulation of excitatory synaptic inputs is often an effective way of inducing robust eCB-STD. When sufficient glutamate is released from excitatory presynaptic terminals by repetitive stimulation, it can induce postsynaptic depolarization mediated by AMPA receptors and resultant [Ca.sup.2+] elevation, and simultaneous activation of mGluR. Each of these three events may be capable of triggering endocannabinoid release by itself. Synaptically-driven eCB-STD may thus involve CaER, RER or [Ca.sup.2+]-assisted RER, depending on stimulation and recording conditions. At parallel fiber to Purkinje cell synapses in the cerebellum, eCB-STD can be induced by a brief burst of parallel fiber stimulation (5-10 pulses at 50-100 Hz). (61)), (69)) This synaptically-driven eCB-STD required both [Ca.sup.2+] elevation and mGluR1 activation in postsynaptic Purkinje cells, indicating predominant contribution of [Ca.sup.2+]-assisted RER. (61)) In contrast, eCB-STD induced by intense parallel fiber stimulation (100 pulses at 100 Hz) was resistant to an mGluR1 antagonist, indicating that contribution of CaER is predominant. (61)) Furthermore, Brenowitz and Regehr reported that parallel fiber to Purkinje cell synapses undergo eCB-STD when subthreshold parallel fiber stimulation was combined with stimulation of climbing fiber, the other excitatory input to Purkinje cells. (70)) Climbing fiber inputs induce depolarization and resultant [Ca.sup.2+] elevation in Purkinje cells, whereas parallel fiber stimulation activates mGluR1 concentrated postsynaptically, which fulfills the requirements of [Ca.sup.2+]-assisted RER. On the other hand, at inhibitory synapses on Purkinje cells, eCB-STD can be induced by intense climbing fiber stimulation (50 pulses at 5Hz). (71)) This heterosynaptic eCB-STD is thought to be dependent on endocannabinoids released from Purkinje cells by climbing fiber stimulation presumably through CaER.

Degradation of 2-AG after eCB-STD. Duration of eCB-STD is dependent on the speed of degradation and removal of 2-AG around synaptic terminals. Since about 85% of 2-AG is hydrolyzed into arachidonic acid and glycerol by MGL, (38)) the localization of this enzyme was thought to be crucial for determining the duration of eCB-STD. Immunohistochemical data indicate that the expression of MGL is highly heterogeneous, being very rich at particular types of presynaptic terminals but totally absent at others. (21)), (72)) MGL is also present in astrocypes. (21)), (72)) Tanimura et al. recently reported that eCB-STD is prolonged rather non-selectively at both MGL-positive and MGL-negative synapses in the cerebellum of MGL knockout mice. (72)) This study also shows that eCB-STD is prolonged in the cerebellum of conditional MGL knockout mice in which MGL is expressed only in astroglia. (72)) This study clearly indicates that 2-AG is degraded in a synapse non-specific manner by MGL concentrated in particular cell types including astroglia. (72))

4. On-demand vs. pre-formed

2-AG is generally thought to be synthesized "on demand" upon cellular stimulation. However, this "on-demand synthesis model" was recently challenged and an alternative model was proposed. (73)), (74)) According to this model, 2-AG is constitutively synthesized by DGL[alpha] and pooled within cells and mobilized from this hypothetical "pre-formed 2-AG pools" upon stimulation without the contribution of DGL[alpha]. (73)), (74)) The reason for developing this new model was to reconcile the apparent discrepancy between the results from DGL[alpha] knockout mice and those from pharmacological blockade of DGL. Three groups independently generated DGL[alpha] knockout mice and they consistently exhibit complete loss of eCBSTD. (48)) (-50)) In contrast, the reported effects of acute pharmacological blockade of DGL on eCB-STD were highly controversial. (73)) A classical DGL inhibitor, tetrahydrolipstatin (THL), inhibited eCB-STD in some studies, (28)), (75)) (-77)) but not in others. (78)), (79)) Inconsistent results were also reported regarding a novel potent DGL inhibitor, oMDM-188. This compound failed to inhibit DSI in one study,') whereas it inhibited DSI but not mGluR-driven STD in another study. (77)) Recently, Hashimotodani et al., systemati cally reexamined the effects of OMDM-188 on eCBSTD induced by CaER, basal RER, [Ca.sup.2+]-assisted RER and synaptically-driven eCB release in acute slices of the hippocampus, cerebellum and striatum as well as in cultured hippocampal neurons. (81)) The data clearly demonstrate that all forms of eCB-STD examined, including DSI, DSE, receptor-driven eCBSTD and synaptically-driven eCB-STD, are consistently blocked by the treatment with OMDM-188. (81)) Importantly, application of OMDM-188 as short as 2 min was sufficient to block DSI in cultured hippocampal neurons. (81)) This study has confirmed that there is no discrepancy in the results between genetic and pharmacological blockade of DGL, and strongly support the "on-demand synthesis model".

The reasons for the inconsistent results in previous studies regarding the effect of DGL inhibitors are unclear. One possibility is insufficient penetration of these compounds into brain tissues. Since OMDM-188 and THL are highly lipophilic, they are difficult to penetrate into brain slices. The effectiveness of these DGL inhibitors therefore depends on whether their targets (e.g., soma or dendrites) are located near the surface or in the depth of slices. Another possibility is related to whether DGL activity is rate limiting for 2-AG production or not. For example, if DGL activity is rate limiting for CaER, but not for RER, these two types of eCB-STD can have different sensitivity to the DGL inhibitors. (77))

5. Endocannabinoid-mediated long-term depression (eCB-LTD)

Long-term depression (LTD) of synaptic transmission dependent on retrograde endocannabinoid signaling (eCB-LTD) has been found in several areas of the brain. Because an excellent review focusing on eCB-LTD is available, (29)) its main features will be briefly summarized here. eCB-LTD occurs at excitatory synapses in the dorsal striatum, nucleus accumbens, cerebral cortex, dorsal cochlear nucleus, cerebellum, and hippocampus, and at inhibitory synapses in the hippocampus, amygdala and ventral tegmental area. (29)) eCB-LTD is induced mostly by repetitive afferent stimulation with or without postsynaptic depolarization, and also by postsynaptic firing (16)), (82)) (for review, see refs. 12, 13). Evidence suggests that, eCB-LTD is mediated by 2-AG in most cases and that eCB-LTD and eCB-STD share the same molecular mechanisms for 2-AG release (i.e., CaER, basal RER and [Ca.sup.2+]-assisted RER) (Fig. 4). Typically, afferent stimulation causes glutamate release from excitatory presynaptic terminals, activates AMPA receptors and mGluRs on the postsynaptic membrane, and induces 2-AG release. 2-AG then activates C[B.sub.1]Rs on the same presynaptic terminals releasing glutamate (homosynaptic) or neighboring presynaptic terminals (heterosynaptic). eCB-STD can be induced by a brief burst of afferent stimulation and resultant release of 2-AG that causes transient activation of presynaptic C[B.sub.1]Rs. In contrast, eCB-LTD requires a long-lasting afferent stimulation for several minutes (5 to 10 minutes) and resultant release of 2-AG that causes persistent activation of C[B.sub.1]Rs. Importantly, LTD induced by spike timing stimulation in the rodent somatosensory cortex has been reported to involve endocannabinoid signaling. (83) This form of spike-timing dependent plasticity may underlie development and critical period plasticity of the whisker map in the rodent somatosensory cortex. (84))

Although presynaptic mechanisms of eCB-LTD have not been fully elucidated, inhibition of the cAMP/PKA pathway seems to be crucial. (12)), (15)) Involvement of calcineurin, the small GTP-binding protein Rab3, and Rab3-interacting molecule 1, (RIMl[alpha]), has also been suggested (12)), (15)) (Fig. 4). In most, if not all cases, activation of C[B.sub.1]R is not enough to induce LTD, and an additional presynaptic mechanism(s) is required, (85)) such as presynaptic electrical activity, (86), 87)) [Ca.sup.2+] elevation, (86), 87)) NMDAR activation, (88)) [M.sub.2] receptor activation (89)) and/or [D.sub.2] receptor activation. (90), 91)) This associative feature may ensure the selective induction of eCB-LTD at active synapses. For example, presynaptic activity induces [Ca.sup.2+] elevation and leads to activation of calcineurin, which shifts the phosphorylation/dephosphorylation balance towards dephosphorylation in concert with reduced PKA activity by C[B.sub.1]R activation. The resulting decrease in phosphorylation of target proteins causes suppression of transmitter release selectively from presynaptic terminals that have been active during C[B.sub.1]R activation (Fig. 4).

6. Roles of anandamide in synaptic modulation

Several recent studies demonstrate that the other major endocannabinoid, anandamide, also contributes to the modulation of synaptic transmission. In particular, anandamide-mediated LTD has been reported in several brain areas. (92)-96)) Two distinct types of eCB-LTD has been reported in medium spiny neurons (MSNs) of the indirect pathway in the dorsolateral striatum. (95)) Low frequency stimulation induces LTD mediated by 2-AG, whereas high frequency stimulation induces C[B.sub.1]-dependent LTD that is mediated by anandamide. (95)) Since anandamide is a partial agonist for C[B.sub.1]R and a full agonist for TRPV1, it may contribute to LTD through two distinct pathways involving C[B.sub.1]R and TRPV1. In line with this notion, Grueter et al. suggested that low frequency stimulation induces anandamide-mediated LTD, partly by activating presynaptic C[B.sub.1]Rs and partly by activating postsynaptic TRPV1 channels in indirect pathway MSNs of the nucleus accumbens. (93)) In the dentate gyrus, Chavez et al. reported that pairing stimulation induces LTD that is C[B.sub.1]-independent and TRPV1-dependent. (92)) They showed this LTD was expressed postsynaptically and mediated by anandamide. (92)) In contrast, Puente et al. suggested that 2-AG mediates both eCB-STD and eCB-LTD in the striatum, whereas 2-AG and anandamide mediate eCB-STD and TRPV1-dependent LTD, respectively, in the extended amygdala. (96)) Besides contribution to LTD induction, anandamide has been shown to generate the endocannabinoid tone. Experimental evidence suggests that it contributes to homeostatic plasticity in the hippocampus (97) and the regulation of activity along hypothalamic-pituitary-adrenal (HPA) axis in the amygdala. (98)) Taking together, the two major endocannabinoids, 2-AG and anandamide, are considered to mediate different types of signals for synaptic modulation. (99) 2-AG primarily transmits a rapid, transient, point-to-point retrograde endocannabinoid signal. In contrast, anandamide may mediate a relatively slow, retrograde or non-retrograde endocannabinoid signal and/or may function as a vanilloid agonist.

7. Plasticity of the endocannabinoid system

As described in previous sections, the endocannabinoid system involves various molecules including voltage-gated [Ca.sup.2+] channels, [G.sub.q/11]-coupled receptors, PLC[beta]s, DGL[alpha], MGL and C[B.sub.1]R. Therefore any changes in their functional activities, expression levels or subcellular distribution can influence the performance of the endocannabinoid system. Recent studies suggest that the endocannabinoid system is regulated by various postsynaptic and presynaptic receptors, and also influenced by environmental and pathological factors. (100)) For example, in human and rodents are known to cause behavioral tolerance to cannabinoids, (25)), (101)) which is considered to result from down-regulation of C[B.sub.1]R. Furthermore, MGL knockout mice and mice with chronic administration of an MGL inhibitor demonstrate cannabinoid tolerance including reduced antinociceptive and hypothermic responses to [[DELTA].sup.9]-THC. (102)) These symptoms are attributed to chronic activation of C[B.sub.1]Rs by excess 2-AG and resultant down-regulation of these receptors.

Receptors on the postsynaptic membrane can influence endocannabinoid release. Lerner and Kreitzer showed that postsynaptic [D.sub.2] and [A.sub.2A] receptors on MSNs of the striatum regulated endocannabinoid release and eCB-LTD positively and negatively, respectively, by modulating mGluR signaling through regulator of G protein signaling 4 (RGS4). (95)) On the other hand, Shonesy et al. reported that enzymatic activity of DGL[alpha] was reduced after phosphorylation by CaMKII, (103)) suggesting the possibility that DGL[alpha] might be regulated by CaMKII-involving signals. On the presynaptic side, C[B.sub.1]R signaling has been shown to be influenced by the activity of colocalized presy naptic receptors. In the hippocampus, Hoffman et al. reported that tonic activation of [A.sub.1] receptors by endogenous adenosine inhibits presynaptic C[B.sub.1] signaling. (104)) Because C[B.sub.1] and [A.sub.1] receptors are both [G.sub.i/o]-coupled, they may use overlapping sets of Go subunits and interact with each other.

Endocannabinoid signaling can also be influenced by environmental factors. For example, stress can alter the endocannabinoid system through HPA axis. Conversely, the endocannabinoid system is known to regulate the HPA axis under both basal and stressful conditions. Several studies indicate that stress-induced HPA axis activity can alter the endocannabinoid system, either positively or negatively depending on the brain region, age and conditions of stress, in several brain regions including the hippocampus, amygdala, hypothalamus, nucleus accumbens and prefrontal cortex. (98), 100), 105)) For example, repetitive immobilization stress is reported to cause down-regulation of C[B.sub.1]R and impair DSI/DSE in the paraventricular nucleus of the hypothalamus. (106)) In contrast, chronic stress caused a partial down-regulation of MGL and enhanced DSI in the basolateral amygdala. (107)) Interestingly, acute restraint stress switched C[B.sub.1]-dependent plasticity from LTD to LTP in the bed nucleus of the stria terminalis, and this stress-elicited shift in plasticity was controlled by C[B.sub.1]-Rs on excitatory terminals. (108)) Molecular and neural circuit mechanisms of these stress-induced plastic changes in the endocannabinoid system remain to be elucidated.

8. Conclusion

In the last two decades, great advances have been made in the cannabinoid research. In 1990s, biochemical studies have identified cannabinoid receptors (C[B.sub.1]R and C[B.sub.2]R) and endocannabinoids (anandamide, 2-AG and others), and characterized the enzymes involved in generation and degradation of endocannabinoids, some of which have been cloned successfully. In the next decade, electrophysiological studies revealed how endocannabinoids are released from neurons and induce short-term and long-term forms of synaptic plasticity. The endocannabinoid 2-AG is produced from membrane lipids upon postsynaptic [Ca.sup.2+] elevation and/or activation of [G.sub.q/11]-coupled receptors, and released from postsynaptic neurons. The released 2-AG acts retrogradely onto presynaptic C[B.sub.1]R and induces suppression of neurotransmitter release. After causing synaptic suppression, 2-AG is degraded in a synapse nonspecific manner by MGL concentrated in particular cell types including astroglia. Postsynaptic COX-2 and ABHD6 may also contributes to inactivation of 2-AG. Besides 2-AG, anandamide has been shown to mediate a certain form of eCB-LTD as well as eCB-independent and TRPV1-dependent form of LTD. It has also been demonstrated that the endocannabinoid system itself is plastic, which can be either upor down-regulated by experimental or environmental conditions. Although endocannabinoid signaling is involved in various forms of short-term and long-term synaptic modulation, it remains largely unknown how each form contributes to particular aspects of neural functions. Future studies will elucidate the molecular, cellular and neural circuit mechanisms as to how endocannabinoid-mediated synaptic modulation contributes to various brain functions and the pathophysiology of neuropsychiatric disorders.

doi: 10.2183/pjab.90.235


The author thanks Yuki Hashimotodani for valuable comments on this review article. The author expresses his sincere thank to all the collaborators concerned with the studies described in this review. This work has been supported in part by Grants-inAid for Scientific Research (21220006 and 25000015), and the Strategic Research Program for Brain Sciences (Development of Biomarker Candidates for Social Behavior) from the Ministry of Education, Culture, Sports, Science and Technology of Japan.


1) Iversen, L. (2000) The Science of Marijuana. Oxford University Press, Inc., New York.

2) Katzung, B.G., Masters, S.B. and Trevor, A.J. (2009) Basic and Clinical Pharmacology. MaGraw-Hill Companies, Inc., New York.

3) Gaoni, Y. and Mechoulam, R. (1964) Isolation, structure and partial synthesis of an active constituent of hashish. J. Am. Chem. Soc. 86, 1646-1647.

4) Matsuda, L.A., Lolait, S.J., Brownstein, M.J., Young, A.C. and Bonner, T.I. (1990) Structure of a cannabinoid receptor and functional expression of the cloned cDNA. Nature 346, 561-564.

5) Munro, S., Thomas, K.L. and Abu-Shaar, M. (1993) Molecular characterization of a peripheral receptor for cannabinoids. Nature 365, 61-65.

6) Devane, W.A., Hanus, L., Breuer, A., Pertwee, R.G., Stevenson, L.A., Griffin, G., Gibson, D., Mandelbaum, A., Etinger, A. and Mechoulam, R. (1992) Isolation and structure of a brain constituent that binds to the cannabinoid receptor. Science 258, 1946-1949.

7) Mechoulam, R., Ben-Shabat, S., Hanus, L., Ligumsky, M., Kaminski, N.E., Schatz, A.R., Gopher, A., Almog, S., Martin, B.R., Compton, D.R., Pertwee, R.G., Griffin, G., Bayewitch, M., Barg, J. and Vogel, Z. (1995) Identification of an endogenous 2-monoglyceride, present in canine gut, that binds to cannabinoid receptors. Biochem. Pharmacol. 50, 83-90.

8) Sugiura, T., Kondo, S., Sukagawa, A., Nakane, S., Shinoda, A., Itoh, K., Yamashita, A. and Waku, K. (1995) 2-Arachidonoylglycerol: a possible endogenous cannabinoid receptor ligand in brain. Biochem. Biophys. Res. Commun. 215, 89-97.

9) Kreitzer, A.C. and Regehr, W.G. (2001) Retrograde inhibition of presynaptic calcium influx by endogenous cannabinoids at excitatory synapses onto Purkinje cells. Neuron 29, 717-727.

10) Ohno-Shosaku, T., Maejima, T. and Kano, M. (2001) Endogenous cannabinoids mediate retrograde signals from depolarized postsynaptic neurons to presynaptic terminals. Neuron 29, 729738.

11) Wilson, R.I. and Nicoll, R.A. (2001) Endogenous cannabinoids mediate retrograde signalling at hippocampal synapses. Nature 410, 588-592.

12) Castillo, P.E., Younts, T.J., Chavez, A.E. and Hashimotodani, Y. (2012) Endocannabinoid signaling and synaptic function. Neuron 76, 70-81.

13) Kano, M., Ohno-Shosaku, T., Hashimotodani, Y., Uchigashima, M. and Watanabe, M. (2009) Endocannabinoid-mediated control of synaptic transmission. Physiol. Rev. 89, 309-380.

14) Katona, I. and Freund, T.F. (2012) Multiple functions of endocannabinoid signaling in the brain. Annu. Rev. Neurosci. 35, 529-558.

15) Ohno-Shosaku, T., Tanimura, A., Hashimotodani, Y. and Kano, M. (2012) Endocannabinoids and retrograde modulation of synaptic transmission. Neuroscientist 18, 119-132.

16) Gerdeman, G.L., Ronesi, J. and Lovinger, D.M. (2002) Postsynaptic endocannabinoid release is critical to long-term depression in the striatum. Nat. Neurosci. 5, 446-451.

17) Robbe, D., Kopf, M., Remaury, A., Bockaert, J. and Manzoni, O.J. (2002) Endogenous cannabinoids mediate long-term synaptic depression in the nucleus accumbens. Proc. Natl. Acad. Sci. U.S.A. 99, 8384-8388.

18) Katona, I., Urban, G.M., Wallace, M., Ledent, C., Jung, K.M., Piomelli, D., Mackie, K. and Freund, T.F. (2006) Molecular composition of the endocannabinoid system at glutamatergic synapses. J. Neurosci. 26, 5628-5637.

19) Kawamura, Y., Fukaya, M., Maejima, T., Yoshida, T., Miura, E., Watanabe, M., Ohno-Shosaku, T. and Kano, M. (2006) The C[B.sub.1] cannabinoid receptor is the major cannabinoid receptor at excitatory presynaptic sites in the hippocampus and cerebellum. J. Neurosci. 26, 2991-3001.

20) Uchigashima, M., Narushima, M., Fukaya, M., Katona, I., Kano, M. and Watanabe, M. (2007) Subcellular arrangement of molecules for 2-arachidonoyl-glycerol-mediated retrograde signaling and its physiological contribution to synaptic modulation in the striatum. J. Neurosci. 27, 3663-3676.

21) Uchigashima, M., Yamazaki, M., Yamasaki, M., Tanimura, A., Sakimura, K., Kano, M. and Watanabe, M. (2011) Molecular and morphological configuration for 2-arachidonoylglycerolmediated retrograde signaling at mossy cellgranule cell synapses in the dentate gyrus. J. Neurosci. 31, 7700-7714.

22) Yoshida, T., Fukaya, M., Uchigashima, M., Miura, E., Kamiya, H., Kano, M. and Watanabe, M. (2006) Localization of diacylglycerol lipase-a around postsynaptic spine suggests close proximity between production site of an endocannabinoid, 2-arachidonoyl-glycerol, and presynaptic cannabinoid C[B.sub.1] receptor. J. Neurosci. 26, 4740-4751.

23) Yoshida, T., Uchigashima, M., Yamasaki, M., Katona, I., Yamazaki, M., Sakimura, K., Kano, M., Yoshioka, M. and Watanabe, M. (2011) Unique inhibitory synapse with particularly rich endocannabinoid signaling machinery on pyramidal neurons in basal amygdaloid nucleus. Proc. Natl. Acad. Sci. U.S.A. 108, 3059-3064.

24) Herkenham, M., Lynn, A.B., Johnson, M.R., Melvin, L.S., de Costa, B.R. and Rice, K.C. (1991) Characterization and localization of cannabinoid receptors in rat brain: a quantitative in vitro autoradiographic study. J. Neurosci. 11, 563-583.

25) Mechoulam, R. and Parker, L.A. (2013) The endocannabinoid system and the brain. Annu. Rev. Psychol. 64, 21-47.

26) Alger, B.E. (2002) Retrograde signaling in the regulation of synaptic transmission: focus on endocannabinoids. Prog. Neurobiol. 68, 247-286.

27) Freund, T.F., Katona, I. and Piomelli, D. (2003) Role of endogenous cannabinoids in synaptic signaling. Physiol. Rev. 83, 1017-1066.

28) Hashimotodani, Y., Ohno-Shosaku, T. and Kano, M. (2007) Endocannabinoids and synaptic function in the CNS. Neuroscientist 13, 127-137.

29) Heifets, B.D. and Castillo, P.E. (2009) Endocanna binoid signaling and long-term synaptic plasticity. Annu. Rev. Physiol. 71, 283-306.

30) Piomelli, D. (2003) The molecular logic of endocan nabinoid signalling. Nat. Rev. Neurosci. 4, 873884.

31) Katona, I., Sperlagh, B., Sik, A., Kafalvi, A., Vizi, E.S., Mackie, K. and Freund, T.F. (1999) Presynaptically located C[B.sub.1] cannabinoid receptors regulate GABA release from axon terminals of specific hippocampal interneurons. J. Neurosci. 19, 4544-4558.

32) Turu, G. and Hunyady, L. (2010) Signal trans duction of the C[B.sub.1] cannabinoid receptor. J. Mol. Endocrinol. 44, 75-85.

33) Starowicz, K., Nigam, S. and Di Marzo, V. (2007) Biochemistry and pharmacology of endovanilloids. Pharmacol. Ther. 114, 13-33.

34) Okamoto, Y., Wang, J., Morishita, J. and Ueda, N. (2007) Biosynthetic pathways of the endocannabinoid anandamide. Chem. Biodivers. 4, 1842-1857.

35) Cravatt, B.F., Giang, D.K., Mayfield, S.P., Boger, D.L., Lerner, R.A. and Gilula, N.B. (1996) Molecular characterization of an enzyme that degrades neuromodulatory fatty-acid amides. Nature 384, 83-87.

36) Sugiura, T., Kishimoto, S., Oka, S. and Gokoh, M. (2006) Biochemistry, pharmacology and physiology of 2-arachidonoylglycerol, an endogenous cannabinoid receptor ligand. Prog. Lipid Res. 45, 405-446.

37) Bisogno, T., Howell, F., Williams, G., Minassi, A., Cascio, M.G., Ligresti, A., Matias, I., SchianoMoriello, A., Paul, P., Williams, E.J., Gangadharan, U., Hobbs, C., Di Marzo, V. and Doherty, P. (2003) Cloning of the first sn1-DAG lipases points to the spatial and temporal regulation of endocannabinoid signaling in the brain. J. Cell Biol. 163, 463-468.

38) Blankman, J.L., Simon, G.M. and Cravatt, B.F. (2007) A comprehensive profile of brain enzymes that hydrolyze the endocannabinoid 2-arachidonoylglycerol. Chem. Biol. 14, 1347-1356.

39) Marrs, W.R., Blankman, J.L., Horne, E.A., Thomazeau, A., Lin, Y.H., Coy, J., Bodor, A.L., Muccioli, G.G., Hu, S.S., Woodruff, G., Fung, S., Lafourcade, M., Alexander, J.P., Long, J.Z., Li, W., Xu, C., Moller, T., Mackie, K., Manzoni, O.J., Cravatt, B.F. and Stella, N. (2010) The serine hydrolase ABHD6 controls the accumulation and efficacy of 2-AG at cannabinoid receptors. Nat. Neurosci. 13, 951-957.

40) Fiskerstrand, T., H'mida-Ben Brahim, D., Johansson, S., M'zahem, A., Haukanes, B.I., Drouot, N., Zimmermann, J., Cole, A.J., Vedeler, C., Bredrup, C., Assoum, M., Tazir, M., Klockgether, T., Hamri, A., Steen, V.M., Boman, H., Bindoff, L.A., Koenig, M. and Knappskog, P.M. (2010) Mutations in ABHD12 cause the neurodegenerative disease PHARC: An inborn error of endocannabinoid metabolism. Am. J. Hum. Genet. 87, 410-417.

41) Vandevoorde, S. and Lambert, D.M. (2007) The multiple pathways of endocannabinoid metabolism: a zoom out. Chem. Biodivers. 4, 1858-1881.

42) Kaufmann, W.E., Worley, P.F., Pegg, J., Bremer, M. and Isakson, P. (1996) COX-2, a synaptically induced enzyme, is expressed by excitatory neurons at postsynaptic sites in rat cerebral cortex. Proc. Natl. Acad. Sci. U.S.A. 93, 23172321.

43) Ohno-Shosaku, T., Hashimotodani, Y., Ano, M., Takeda, S., Tsubokawa, H. and Kano, M. (2007) Endocannabinoid signalling triggered by NMDA receptor-mediated calcium entry into rat hippocampal neurons. J. Physiol. 584, 407-418.

44) Llano, I., Leresche, N. and Marty, A. (1991) Calcium entry increases the sensitivity of cerebellar Purkinje cells to applied GABA and decreases inhibitory synaptic currents. Neuron 6, 565-574.

45) Pitler, T.A. and Alger, B.E. (1992) Postsynaptic spike firing reduces synaptic GABAa responses in hippocampal pyramidal cells. J. Neurosci. 12, 4122-4132.

46) Chevaleyre, V., Takahashi, K.A. and Castillo, P.E. (2006) Endocannabinoid-mediated synaptic plasticity in the CNS. Annu. Rev. Neurosci. 29, 3776.

47) Hashimotodani, Y., Ohno-Shosaku, T. and Kano, M. (2007) Presynaptic monoacylglycerol lipase activity determines basal endocannabinoid tone and terminates retrograde endocannabinoid signaling in the hippocampus. J. Neurosci. 27, 12111219.

48) Gao, Y., Vasilyev, D.V., Goncalves, M.B., Howell, F. V., Hobbs, C., Reisenberg, M., Shen, R., Zhang, M.Y., Strassle, B.W., Lu, P., Mark, L., Piesla, M.J., Deng, K., Kouranova, E.V., Ring, R.H., Whiteside, G.T., Bates, B., Walsh, F.S., Williams, G. , Pangalos, M.N., Samad, T.A. and Doherty, P. (2010) Loss of retrograde endocannabinoid signaling and reduced adult neurogenesis in diacylglycerol lipase knock-out mice. J. Neurosci. 30, 20172024.

49) Tanimura, A., Yamazaki, M., Hashimotodani, Y., Uchigashima, M., Kawata, S., Abe, M., Kita, Y., Hashimoto, K., Shimizu, T., Watanabe, M., Sakimura, K. and Kano, M. (2010) The endocannabinoid 2-arachidonoylglycerol produced by diacylglycerol lipase [alpha] mediates retrograde suppression of synaptic transmission. Neuron 65, 320327.

50) Yoshino, H., Miyamae, T., Hansen, G., Zambrowicz, B., Flynn, M., Pedicord, D., Blat, Y., Westphal, R.S., Zaczek, R., Lewis, D.A. and GonzalezBurgos, G. (2011) Postsynaptic diacylglycerol lipase mediates retrograde endocannabinoid suppression of inhibition in mouse prefrontal cortex. J. Physiol. 589, 4857-4884.

51) Maejima, T., Hashimoto, K., Yoshida, T., Aiba, A. and Kano, M. (2001) Presynaptic inhibition caused by retrograde signal from metabotropic glutamate to cannabinoid receptors. Neuron 31, 463-475.

52) Kim, J., Isokawa, M., Ledent, C. and Alger, B.E. (2002) Activation of muscarinic acetylcholine receptors enhances the release of endogenous cannabinoids in the hippocampus. J. Neurosci. 22, 10182-10191.

53) Fukudome, Y., Ohno-Shosaku, T., Matsui, M., Omori, Y., Fukaya, M., Tsubokawa, H., Taketo, M.M., Watanabe, M., Manabe, T. and Kano, M. (2004) Two distinct classes of muscarinic action on hippocampal inhibitory synapses: [M.sub.2] -mediated direct suppression and [M.sub.1]/[M.sub.3]-mediated indirect suppression through endocannabinoid signalling. Eur. J. Neurosci. 19, 2682-2692.

54) Straiker, A. and Mackie, K. (2007) Metabotropic suppression of excitation in murine autaptic hippocampal neurons. J. Physiol. 578, 773-785.

55) Narushima, M., Uchigashima, M., Fukaya, M., Matsui, M., Manabe, T., Hashimoto, K., Watanabe, M. and Kano, M. (2007) Tonic enhancement of endocannabinoid-mediated retrograde suppression of inhibition by cholinergic interneuron activity in the striatum. J. Neurosci. 27, 496-506.

56) Best, A.R. and Regehr, W.G. (2008) Serotonin evokes endocannabinoid release and retrogradely suppresses excitatory synapses. J. Neurosci. 28, 6508-6515.

57) Haj-Dahmane, S. and Shen, R.Y. (2005) The wake promoting peptide orexin-B inhibits glutamatergic transmission to dorsal raphe nucleus serotonin neurons through retrograde endocannabinoid signaling. J. Neurosci. 25, 896-905.

58) Hirasawa, M., Schwab, Y., Natah, S., Hillard, C.J., Mackie, K., Sharkey, K.A. and Pittman, Q.J. (2004) Dendritically released transmitters cooperate via autocrine and retrograde actions to inhibit afferent excitation in rat brain. J. Physiol. 559, 611-624.

59) Watanabe, M., Nakamura, M., Sato, K., Kano, M., Simon, M.I. and Inoue, Y. (1998) Patterns of expression for the mRNA corresponding to the four isoforms of phospholipase C[beta] in mouse brain. Eur. J. Neurosci. 10, 2016-2025.

60) Hashimotodani, Y., Ohno-Shosaku, T., Tsubokawa, H., Ogata, H., Emoto, K., Maejima, T., Araishi, K., Shin, H.S. and Kano, M. (2005) Phospholipase C[beta] serves as a coincidence detector through its [Ca.sup.2+] dependency for triggering retrograde endocannabinoid signal. Neuron 45, 257-268.

61) Maejima, T., Oka, S., Hashimotodani, Y., Ohno Shosaku, T., Aiba, A., Wu, D., Waku, K., Sugiura, T. and Kano, M. (2005) Synaptically driven endocannabinoid release requires [Ca.sup.2+]assisted metabotropic glutamate receptor subtype 1 to phospholipase C[beta]4 signaling cascade in the cerebellum. J. Neurosci. 25, 6826-6835.

62) Melis, M., Perra, S., Muntoni, A.L., Pillolla, G., Lutz, B., Marsicano, G., Di Marzo, V., Gessa, G.L. and Pistis, M. (2004) Prefrontal cortex stimulation induces 2-arachidonoyl-glycerol-mediated suppression of excitation in dopamine neurons. J. Neurosci. 24, 10707-10715.

63) Safo, P.K. and Regehr, W.G. (2005) Endocannabi noids control the induction of cerebellar LTD. Neuron 48, 647-659.

64) Hashimotodani, Y., Ohno-Shosaku, T. and Kano, M. (2007) [Ca.sup.2+]-assisted receptor-driven endocannabinoid release: mechanisms that associate presynaptic and postsynaptic activities. Curr. Opin. Neurobiol. 17, 360-365.

65) Ohno-Shosaku, T., Matsui, M., Fukudome, Y., Shosaku, J., Tsubokawa, H., Taketo, M.M., Manabe, T. and Kano, M. (2003) Postsynaptic [M.sub.1] and [M.sub.3] receptors are responsible for the muscarinic enhancement of retrograde endocannabinoid signalling in the hippocampus. Eur. J. Neurosci. 18, 109-116.

66) Ohno-Shosaku, T., Shosaku, J., Tsubokawa, H. and Kano, M. (2002) Cooperative endocannabinoid production by neuronal depolarization and group I metabotropic glutamate receptor activation. Eur. J. Neurosci. 15, 953--961.

67) Lemtiri-Chlieh, F. and Levine, E.S. (2010) BDNF evokes release of endogenous cannabinoids at layer 2/3 inhibitory synapses in the neocortex. J. Neurophysiol. 104, 1923-1932.

68) Nevian, T. and Sakmann, B. (2006) Spine [Ca.sup.2+] signaling in spike-timing-dependent plasticity. J. Neurosci. 26, 11001-11013.

69) Brown, S.P., Brenowitz, S.D. and Regehr, W.G. (2003) Brief presynaptic bursts evoke synapsespecific retrograde inhibition mediated by endogenous cannabinoids. Nat. Neurosci. 6, 1048-1057.

70) Brenowitz, S.D. and Regehr, W.G. (2005) Associa tive short-term synaptic plasticity mediated by endocannabinoids. Neuron 45, 419-431.

71) Urbanski, M.J., Kovacs, F.E. and Szabo, B. (2010) Endocannabinoid-mediated synaptically evoked suppression of GABAergic transmission in the cerebellar cortex. Neuroscience 169, 1268-1278.

72) Tanimura, A., Uchigashima, M., Yamazaki, M., Uesaka, N., Mikuni, T., Abe, M., Hashimoto, K., Watanabe, M., Sakimura, K. and Kano, M. (2012) Synapse type-independent degradation of the endocannabinoid 2-arachidonoylglycerol after retrograde synaptic suppression. Proc. Natl. Acad. Sci. U.S.A. 109, 12195-12200.

73) Alger, B.E. and Kim, J. (2011) Supply and demand for endocannabinoids. Trends Neurosci. 34, 304315.

74) Min, R., Di Marzo, V. and Mansvelder, H.D. (2010) DAG lipase involvement in depolarization-induced suppression of inhibition: does endocannabinoid biosynthesis always meet the demand? Neuroscientist 16, 608-613.

75) Edwards, D.A., Zhang, L. and Alger, B.E. (2008) Metaplastic control of the endocannabinoid system at inhibitory synapses in hippocampus. Proc. Natl. Acad. Sci. U.S.A. 105, 8142-8147.

76) Hashimotodani, Y., Ohno-Shosaku, T., Maejima, T., Fukami, K. and Kano, M. (2008) Pharmacological evidence for the involvement of diacylglycerol lipase in depolarization-induced endocanabinoid release. Neuropharmacology 54, 58-67.

77) Zhang, L., Wang, M., Bisogno, T., Di Marzo, V. and Alger, B.E. (2011) Endocannabinoids generated by [Ca.sup.2+] or by metabotropic glutamate receptors appear to arise from different pools of diacylglycerol lipase. PLoS ONE 6, e16305.

78) Edwards, D.A., Kim, J. and Alger, B.E. (2006) Multiple mechanisms of endocannabinoid response initiation in hippocampus. J. Neurophysiol. 95, 67-75.

79) Szabo, B., Urbanski, M.J., Bisogno, T., Di Marzo, V., Mendiguren, A., Baer, W.U. and Freiman, I. (2006) Depolarization-induced retrograde synaptic inhibition in the mouse cerebellar cortex is mediated by 2-arachidonoylglycerol. J. Physiol. 577, 263-280.

80) Min, R., Testa-Silva, G., Heistek, T.S., Canto, C.B., Lodder, J.C., Bisogno, T., Di Marzo, V., Brussaard, A.B., Burnashev, N. and Mansvelder, H.D. (2010) Diacylglycerol lipase is not involved in depolarization-induced suppression of inhibition at unitary inhibitory connections in mouse hippocampus. J. Neurosci. 30, 2710-2715.

81) Hashimotodani, Y., Ohno-Shosaku, T., Tanimura, A., Kita, Y., Sano, Y., Shimizu, T., Di Marzo, V. and Kano, M. (2013) Acute inhibition of diacylglycerol lipase blocks endocannabinoidmediated retrograde signalling: evidence for ondemand biosynthesis of 2-arachidonoylglycerol. J. Physiol. 591, 4765-4776.

82) Younts, T.J., Chevaleyre, V. and Castillo, P.E. (2013) CA1 pyramidal cell theta-burst firing triggers endocannabinoid-mediated long-term depression at both somatic and dendritic inhibitory synapses. J. Neurosci. 33, 13743-13757.

83) Bender, V.A., Bender, K.J., Brasier, D.J. and Feldman, D.E. (2006) Two coincidence detectors for spike timing-dependent plasticity in somatosensory cortex. J. Neurosci. 26, 4166-4177.

84) Li, L., Bender, K.J., Drew, P.J., Jadhav, S.P., Sylwestrak, E. and Feldman, D.E. (2009) Endocannabinoid signaling is required for development and critical period plasticity of the whisker map in somatosensory cortex. Neuron 64, 537-549.

85) Castillo, P.E. (2012) Presynaptic LTP and LTD of excitatory and inhibitory synapses. Cold Spring Harb. Perspect. Biol. 4, a005728.

86) Heifets, B.D., Chevaleyre, V. and Castillo, P.E. (2008) Interneuron activity controls endocannabinoid-mediated presynaptic plasticity through calcineurin. Proc. Natl. Acad. Sci. U.S.A. 105, 10250-10255.

87) Singla, S., Kreitzer, A.C. and Malenka, R.C. (2007) Mechanisms for synapse specificity during striatal long-term depression. J. Neurosci. 27, 5260-5264.

88) Sjostrom, P.J., Turrigiano, G.G. and Nelson, S.B. (2003) Neocortical LTD via coincident activation of presynaptic NMDA and cannabinoid receptors. Neuron 39, 641-654.

89) Ahumada, J., Fernandez de Sevilla, D., Couve, A., Buno, W. and Fuenzalida, M. (2013) Long-term depression of inhibitory synaptic transmission induced by spike-timing dependent plasticity requires coactivation of endocannabinoid and muscarinic receptors. Hippocampus 23, 14391452.

90) Chiu, C.Q., Puente, N., Grandes, P. and Castillo, P.E. (2010) Dopaminergic modulation of endocannabinoid-mediated plasticity at GABAergic synapses in the prefrontal cortex. J. Neurosci. 30, 7236-7248.

91) Pan, B., Hillard, C.J. and Liu, Q.S. (2008) D2 dopamine receptor activation facilitates endocannabinoid-mediated long-term synaptic depression of GABAergic synaptic transmission in midbrain dopamine neurons via cAMP-protein kinase A signaling. J. Neurosci. 28, 14018-14030.

92) Chavez, A.E., Chiu, C.Q. and Castillo, P.E. (2010) TRPV1 activation by endogenous anandamide triggers postsynaptic long-term depression in dentate gyrus. Nat. Neurosci. 13, 1511-1518.

93) Grueter, B.A., Brasnjo, G. and Malenka, R.C. (2010) Postsynaptic TRPV1 triggers cell typespecific long-term depression in the nucleus accumbens. Nat. Neurosci. 13, 1519-1525.

94) Khlaifia, A., Farah, H., Gackiere, F. and Tell, F. (2013) Anandamide, cannabinoid type 1 receptor, and NMDA receptor activation mediate nonHebbian presynaptically expressed long-term depression at the first central synapse for visceral afferent fibers. J. Neurosci. 33, 12627-12637.

95) Lerner, T.N. and Kreitzer, A.C. (2012) RGS4 is required for dopaminergic control of striatal LTD and susceptibility to parkinsonian motor deficits. Neuron 73, 347-359.

96) Puente, N., Cui, Y., Lassalle, O., Lafourcade, M., Georges, F., Venance, L., Grandes, P. and Manzoni, O.J. (2011) Polymodal activation of the endocannabinoid system in the extended amygdala. Nat. Neurosci. 14, 1542-1547.

97) Kim, J. and Alger, B.E. (2010) Reduction in endocannabinoid tone is a homeostatic mechanism for specific inhibitory synapses. Nat. Neurosci. 13, 592-600.

98) Hill, M.N., Patel, S., Campolongo, P., Tasker, J.G., Wotjak, C.T. and Bains, J.S. (2010) Functional interactions between stress and the endocannabinoid system: from synaptic signaling to behavioral output. J. Neurosci. 30, 14980-14986.

99) Piomelli, D. (2014) More surprises lying ahead. The endocannabinoids keep us guessing. Neuropharmacology 76 Pt B, 228-234.

100) Iremonger, K.J., Wamsteeker Cusulin, J.I. and Bains, J.S. (2013) Changing the tune: plasticity and adaptation of retrograde signals. Trends Neurosci. 36, 471-479.

101) Greydanus, D.E., Hawver, E.K., Greydanus, M.M. and Merrick, J. (2013) Marijuana: current concepts. Front Public Health 1, Article 42.

102) Schlosburg, J.E., Blankman, J.L., Long, J.Z., Nomura, D.K., Pan, B., Kinsey, S.G., Nguyen, P. T., Ramesh, D., Booker, L., Burston, J.J., Thomas, E.A., Selley, D.E., Sim-Selley, L.J., Liu, Q. S., Lichtman, A.H. and Cravatt, B.F. (2010) Chronic monoacylglycerol lipase blockade causes functional antagonism of the endocannabinoid system. Nat. Neurosci. 13, 1113-1119.

103) Shonesy, B.C., Wang, X., Rose, K.L., Ramikie, T.S., Cavener, V.S., Rentz, T., Baucum, A.J. 2nd, Jalan-Sakrikar, N., Mackie, K., Winder, D.G., Patel, S. and Colbran, R.J. (2013) CaMKII regulates diacylglycerol lipase [alpha] and striatal endocannabinoid signaling. Nat. Neurosci. 16, 456-463.

104) Hoffman, A.F., Laaris, N., Kawamura, M., Masino, S.A. and Lupica, C.R. (2010) Control of cannabinoid C[B.sub.1] receptor function on glutamate axon terminals by endogenous adenosine acting at [A.sub.1] receptors. J. Neurosci. 30, 545-555.

105) Senst, L. and Bains, J. (2014) Neuromodulators, stress and plasticity: a role for endocannabinoid signalling. J. Exp. Biol. 217, 102-108.

106) Wamsteeker, J.I., Kuzmiski, J.B. and Bains, J.S. (2010) Repeated stress impairs endocannabinoid signaling in the paraventricular nucleus of the hypothalamus. J. Neurosci. 30, 11188-11196.

107) Patel, S., Kingsley, P.J., Mackie, K., Marnett, L.J. and Winder, D.G. (2009) Repeated homotypic stress elevates 2-arachidonoylglycerol levels and enhances short-term endocannabinoid signaling at inhibitory synapses in basolateral amygdala. Neuropsychopharmacology 34, 2699-2709.

108) Glangetas, C., Girard, D., Groc, L., Marsicano, G., Chaouloff, F. and Georges, F. (2013) Stress switches cannabinoid type-1 (C[B.sub.1]) receptor-dependent plasticity from LTD to LTP in the bed nucleus of the stria terminalis. J. Neurosci. 33, 19657-19663.

By Masanobu KANO * 1, ([dagger]) (Communicated by Masanori OTSUKA, M.J.A.)

* 1 Department of Neurophysiology, Graduate School of Medicine, The University of Tokyo, Tokyo, Japan.

([dagger]) Correspondence should be addressed: M. Kano, Department of Neurophysiology, Graduate School of Medicine, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan (e-mail:

Abbreviations: [[DELTA].sup.9]-THC: [[DELTA].sup.9]-Tetrahydrocannabinol; 2AG: 2-arachidonoylglycerol; ABHD6 and ABHD12: [alpha]/[beta] hydrolase domain 6 and 12; CCK: cholecystokinin; COX-2: cyclooxygenase-2; CaER: [Ca.sup.2+]-driven endocannabinoid release; DGL: diacylglycerol lipase; DHPG: 3,5-dihydroxyphenylglycine; DSE: depolarizationinduced suppression of excitation; DSI: depolarization-induced suppression of inhibition; FAAH: fatty acid amide hydrolase; LTD: long-term depression; MGL: monoacylglycerol lipase; NAPE-PLD: N-arachidonoyl phosphatidylethanolamine-hydrolyzing phospholipase D; PHARC: polyneuropathy, hearing loss, ataxia, retinitis pigmentosa, and cataract; PLC: phospholipase C; RER: receptordriven endocannabinoid release; RIM1a: Rab3-interacting molecule 1[alpha]; STD: short-term depression; THL: tetrahydrolipstatin; TRPC6: transient receptor potential cation channel subfamily C member 6; TRPV1: transient receptor potential vanilloid type 1 receptor; eCB-LTD: endocannabinoid-mediated long-term depression; eCB-STD: endocannabinoid-mediated short-term depression.

(Received Mar. 29, 2014; accepted May 30, 2014)


Masanobu Kano was born at Shizuoka in 1957. He received his medical degree from Tokyo Medical and Dental University in 1982. He completed his PhD at the University of Tokyo, Faculty of Medicine in 1986, where he worked on identification of glutamate receptor subtype involved in cerebellar long-term depression. He became a research associate at Jichi Medical School (Tochigi, Japan) in 1986 and studied cerebellar control of optokinetic eye movements. In 1990, he became independent as an assistant professor at Jichi Medical School, and then joined Max-Planck Institute for biophysical chemistry in Goettingen, Germany, as a visiting researcher. In Goettingen, he learned patch clamping and [Ca.sup.2+] imaging from neurons in brain slices, and discovered long-term potentiation of inhibitory synapse in the cerebellum. He returned to Jichi Medical School in 1992, and started to examine postnatal development and plasticity of cerebellar synapses. He moved to the RIKEN Institute (Wako, Japan) in 1995, and then he became Professor of Physiology at Kanazawa University School of Medicine (Kanazawa, Japan) in 1998. In Kanazawa, his group discovered that endogenous cannabinoids function as a retrograde messenger at synapses. In 2005, he became Professor of Cellular Neuroscience at Osaka University, Graduate School of Medicine. In September 2007, he became Professor of Neurophysiology at the University Tokyo, Graduate School of Medicine.
COPYRIGHT 2014 The Japan Academy
No portion of this article can be reproduced without the express written permission from the copyright holder.
Copyright 2014 Gale, Cengage Learning. All rights reserved.

Article Details
Printer friendly Cite/link Email Feedback
Author:Kano, Masanobu
Publication:Japan Academy Proceedings Series B: Physical and Biological Sciences
Article Type:Report
Geographic Code:9JAPA
Date:Jul 1, 2014
Previous Article:Frontal affinity chromatography: a unique research tool for biospecific interaction that promotes glycobiology.
Next Article:Strategies for eliminating death from gastric cancer in Japan.

Terms of use | Privacy policy | Copyright © 2022 Farlex, Inc. | Feedback | For webmasters |