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Competition for space among sessile marine invertebrates: Changes in HSP70 expression in two pacific cnidarians.

Sergi Rossi (*)

Mark J. Snyder (+)

The role of stress proteins--either constitutive (HSC) or inducible (HSP)--of the HSP70 family in intra-and interspecific competition for space was examined in two sessile Pacific cnidarians. Anthopleura elegantissima, an intertidal anemone, and Corynactis californica, a subtidal corallimorpharian, express HSP70 in the absence of apparent physical stress. HSP70 protein expression is concentrated in the tentacles of A. elegantissima when the animal is exposed to contact with other benthic organisms. Under the same conditions, however, HSP concentrations are similar in the body and tentacles of C. californica. When two different clones of A. elegantissima interact in the field, the outside polyps (warriors) express more HSP70 than the inside ones (2.4 versus 0.6 ng HSP70/[micro]g Protein). When different C. californica clones interact, HSP70 expression in the outside and inside polyps is similar (1.5 versus 1.8 ng HSP70/[micro]g P) and is fairly constant in the corallimorpharian in the different inter specific encounters. HSP70 expression is related to the different kinds of aggression encountered by both cnidarians. HSP70 expression may be involved in the recovery of tissues damaged by the allelochemical, cytotoxical, or corrosive substances produced by different enemies. C. californica clones appear prepared for war, as evidenced by the high constant expression of HSP70 in the polyps. A. elegantissima exhibits differential HSP70 expression depending on the identity of each neighboring intra- or interspecific sessile competitor. We propose that stress proteins can be used to quantify space competition or aggression among sessile marine invertebrates.

Abstract.

Introduction

Space on which to live is often the most limiting resource in marine hard-substratum environments, and patchiness has evolved under the influence of intense competition for living space (Connell, 1961; Pequegnat, 1964; Paine, 1971; Dayton, 1971; Jackson, 1977). Once established, organisms can show aggressive behavior (Chadwick, 1987) that may be especially intense in cryptic environments where free space is almost nonexistent.

In benthic environments, sponges, ectoprocts, cnidarians, and ascidians can produce biologically active substances that may be destructive to enemies during space competition (Whittaker and Feeny, 1973; Uriz et al., 1991). These organisms aggregate in patches that can dominate hard-bottom substrates (Sutherland, 1978; Chornesky, 1983; Chadwick, 1987, 1991; Chadwick and Adams, 1991; Langmead and Chadwick, 1999a, b, among others). Growth is often slow in such organisms, and interactions between competitors are often nonevident. It is difficult to quantify competitive interactions in situ, and the manipulation of organisms is frequently essential to demonstrate the potential effects of space competition (Schoener, 1983). For example, investigators have rarely observed agonistic interactions in wild anemones (A. xanthogrammica), although these organisms frequently exhibit such behavior in forced situations (Sebens, 1984). The quantification of damage from encounters between such organisms and the identification o f potential mechanisms used to counter the effect of such aggression have proved difficult. Most studies have dealt with the organismal responses to the attack and the consequent aggressive behavior displayed by individuals. Few workers have focused on the capacities, and implied mechanisms, for tissue recovery following aggressive interactions. We hypothesize that components of the stress response such as HSPs may provide evidence of the intensity of competitive interactions and are one of the mechanisms by which cnidarians recover from or prepare their tissues for the effects of competitive or aggressive interactions.

HSPs enhance cell survival by reducing the accumulation of damaged or abnormal polypeptides within cells (Feder and Hofmann, 1999). However, whether all wild organisms routinely, occasionally, or seldom express inducible HSPs is unknown. For marine invertebrates, most investigators have examined the effects of thermal variations on constitutive (HSC70) and inducible (HSP70) responses (Feder and Hofmann, 1999). Competitive interactions between sessile organisms can elicit HSP responses due to protein damage following the excretion of harmful substances by one or both competitors (Uriz et al., 1991; Turon et al., 1996; Wiens et al., 1998). One index of tolerance to aggressive sessile organisms could be the presence and abundance of mechanisms (such as HSPs) that would resist or ameliorate the damage inflicted on cellular components by the potential space competitor. Furthermore, once HSP can be related to space competition, no manipulation will be necessary to test such hypotheses. HSP expression could then be a quantitative tool to examine competitive interactions in the field without human interference.

To determine whether HSP expression patterns could be related to competitive interactions in marine hard-bottom sessile invertebrates, two Pacific cnidarians were chosen for study: the intertidal anemone Anthopleura elegantissima and the subtidal corallimorpharian Corynactis californica. A. elegantissima forms contiguous aggregations composed of individuals of a single clone, the products of asexual reproduction (Francis, 1973b; Sebens, 1982a, b). Free zones are created where competition between clones occurs through the outside polyps of the aggregation (called "warriors," Francis, 1973a). Compared with polyps in the center of the clone, the warriors have larger and more abundant acrorhagi (specialized nonfeeding tentacles) and lack mature gonads (Francis, 1973b, 1976). The aggressive response is not directly involved in either defense against predators or capture of prey (Francis, 1973b), but functions in the competition for space. We hypothesize that A. elegantissima warriors may exhibit higher HSP levels than interior clonemates because they interact more frequently with competitors.

In the subtidally distributed C. californica, the polyps have no distinctive roles within each clone (Chadwick, 1987). Although the physiology of this group is not as well understood as that of anemones, several studies have described the competition for space and the specific responses to aggression in corallimorpharians (Chadwick, 1987, 1991; Chadwick and Adams, 1991; Langmead and Chadwick, 1999a, b). Space competition experiments demonstrate that C. californica influences the abundance and population structure of other cnidarians by means of its aggressive behavior (Chadwick, 1987, 1991; Chadwick and Adams, 1991). We sought to determine whether the high aggression in this species is related to elevated HSP levels as preparation for possible damage resulting from such interspecies encounters.

We tested two main hypotheses in this work: first, that stress produced by space competition can induce HSP expression to counter the effects of aggressive neighbors; second, that HSP expression can provide a quantitative assay for space competition in sessile invertebrates.

Materials and Methods

Animals and treatments

Anthopleura elegantissima and Corynactis californica were collected from the Bodega Bay area and held in the running seawater system of the Bodega Marine Laboratory. All animals were held in ambient seawater (13-15 [degrees]C) and fed adult brine shrimp or frozen seafood. The seawater from the Bodega Bay area is considered clean, and the animals used in these experiments are considered to have had minimal contact with anthropogenic chemicals that are known to induce HSP expression (McCain et al., 1988). All experiments (aquarium and field) were done in September-October 1998 and 1999 to avoid seasonal differences in cnidarian behavior. Each experiment, whether forced interactions in an aquarium or in situ interaction, was designed to assess the effects of neighboring competition for space on HSP70 expression.

Forced aquarium experiments

The first experiment examined HSP70 protein expression in A. elegantissima and C. californica in a forced situation. Six isolated polyps of each species (attached to stones, no physical stress induced) were moved into contact with each other (i.e., one polyp of A. elegantissima against one polyp of C. californica). After 24 h, tentacle samples from three individuals of each species were removed and frozen in liquid nitrogen. To quantify the differences between tentacles and body, the other three polyps of each species were sampled 48 h later, frozen in liquid nitrogen, and then assayed for HSP70 level by methods detailed below. As controls, isolated polyp tentacles (n 5-6, no interacting species) of A. elegantissima and C. californica were likewise sampled in the aquarium.

In situ intraspecific competition

We assessed HSP70 expression related to competition for space in a natural environmental situation (i.e., in natural clones in the field). Because collection and transport of animals to artificial holding conditions can stimulate a stress response (Sharp et al., 1994; Roberts et al., 1997), clones of A. elegantissima and C. californica were located and sampled from the Bodega Bay Jetty from a minimum 2 m below the 0 tide level (permanently submerged). This avoided significant desiccation, changes in temperature, fluctuations in salinity and pH, and other effects that are typical of the environment for the intertidal A. elegantissima but not for the subtidal C. californica.

For the A. elegantissima intraspecific competition experiments, clones were located by scuba and photographed (Nikonos V camera, 35-mm lens with macro 1:1 or close-up lens). Polyps of each clone were sampled (n = 3, tentacles) from the outside (touching the competitor) and the inside (touching only the same clone, 10-20 cm from the outside polyps). Samples were dissected, kept in 13 [degrees]C seawater for no longer than 30 min before freezing in liquid nitrogen, and stored at -70 [degrees]C. As a control to assess whether HSP70 levels were affected by the extra 30-min tissue incubation in ambient seawater before freezing, the following experiment was performed. Individual tentacle samples were obtained from three individuals of two clones exposed to elevated temperatures in the intertidal zone (elevated HSP70 is found in these conditions, Snyder and Rossi, unpubl. obs.). Each sample was divided into three parts, of which two were immediately frozen in liquid nitrogen and the third was submerged in ambient se awater for 40 min prior to freezing as above.

For the C. californica intraspecific competition experiments, six clones were located and sampled as above. Color varies greatly between different clonal aggregations, which is useful in distinguishing clones that show potential intraspecific competition. Outside and inside polyps (tentacle crowns) of each clone were sampled to compare interacting (<2.5 mm apart) and non-interacting individuals (5-10 cm apart from the outside ones).

Interspecific competition

To examine the effects that different space competitors in the benthic substrata have on HSP70 protein levels, we chose two genera of algae that compete for space with A. elegantissima and C. californica and two intertidal and two subtidal invertebrates for A. elegantissima and C. californica, respectively. The sampled and photographed anemone clones were always submerged (as described before).

Four clones of A. elegantissima and three of C. californica that were interacting with a calcareous red alga (Lithothamnium sp.) were dissected (outside and inside clone tentacles). Another alga interacting with both cnidarians was a fleshy green alga (Ulva sp.), and six clones of each cnidarian were sampled as above.

In the high subtidal, common space competitors of A. elegantissima are the anemone A. xanthogrammica and the cirriped Balanus amphitrite. Five A. elegantissima clones interacting with A. xanthogrammica were sampled in the outside and inside parts of the clones. For B. amphitrite, three clones competing for space were likewise sampled. For C. californica, the subtidal organisms chosen (sponge Haliclona permollis; ascidian Synoicum parfustis) were considered potentially more aggressive than the fleshy algae. Six C. californica clones were chosen for their clear interactions with H. permollis, and polyps of the outside and inside part of each clone were dissected. For S. parfustis, the interaction of the clones was observed in four populations in the dive area, and outside and inside polyps were sampled.

HSP70 measurements

The western immunoblotting for HSP70 expression was done as follows. Frozen tentacle samples (stored at -70 [degrees]C) were individually homogenized in 0.2 ml of buffer K containing 5 mM Na[HPO.sub.4], 40 mM HEPES (pH 7.4), 5 mM Mg[Cl.sub.2], 70 mM potassium gluconate, 150 mM sorbitol, and 1% SDS. Homogenates were centrifuged 10 min at 10,000 X g, and the supernatants were combined with equal volumes of SDS sample buffer (Laemmli, 1970) and boiled for 5 min. Supernatant protein levels were determined by BioRad DC assay, and 20 [micro]g of tentacle protein was loaded in each gel lane. For each blot, 50 ng of standard HSP70 protein (human, StressGen) was included. Discontinuous SDS gels (1 mm) were 6.2% for the stacking gel and 12% for the resolving gel. After running for 2 h at 150 V, SDS gels were electroblotted onto PDVF membranes (for 1 h at 100 V). The protein bands in each western blot were visualized by staining with Ponceau S. HSP70 protein was detected with mouse monoclonal anti-HSP70 (SPA-822, Stress Gen, Victoria, BC); the secondary antibody was goat-anti-mouse IgG, conjugated to peroxidase (Sigma), and was visualized with ECL reagents (Amersham) and exposure of blots to X-ray film.

Blot band intensities were compared by scanning the X-ray films and analyzing the scans with the NIH Image software package. For each blot, the scanned intensity of the HSP was normalized against the intensities of the HSP70 protein standard from that blot; that is, the NIH Image datum point was divided by the intensity of the HSP70 standard.

Results

Anthopleura elegantissima and Corynactis californica express a single HSC70 or HSP70 protein (Fig. 1). In other eukaryotes, the HSP70-DnaK protein family comprises multiple proteins, more than one of which may be detected by the antibody. For the sake of convenience, we will collectively term these as "HSP70." The inclusion of protease inhibitors did not affect HSP70 levels (Fig. 1A, Anthopleura 1 and 2, a versus b); therefore they were omitted from our studies during the homogenization steps. The 30-min ambient seawater submersion of subtidal tentacle samples prior to freezing had no effect compared with immediate freezing (Fig. 1A, Anthopleura 1 and 2, c versus a and b). In comparing tentacles of the same polyp 24 h after the first forced interaction between the two cnidarian species in the laboratory, no differences were observed (F(3, 8) = 2.0, P < 0.1929) (Fig. 2). Two days later, HSP70 levels in A. elegantissima tentacle were 4 times greater than before (4.0 [+ or -] 0.5 ng HSP70/[micro]g P in the tenta cles; 0.0 [+ or -] 0.1 ng HSP70/[micro]g P in the body, power of test = 0.87), but no differences were detected in C. californica tentacles (1.7 [+ or -] 0.9 ng HSP70/[micro]g P in the tentacles; 0.8 [+ or -] 0.9 ng HSP70/[micro]g P in the body) (Fig. 2). Differences between tentacles and body were found in A. elegantissima but not in C. californica (Fig. 1; F(3, 8) = 18.55, P < 0.0006, power of test = 0.98). Algal symbionts are at the highest concentration in A. elegantissima oral disk (Fitt et al., 1982; Weis and Levine, 1996); these data imply that we are measuring HSP70 responses in animal tissue. No such differences were found in the corallimorpharian, which lacks algal symbionts.

HSP70 levels in isolated polyps were also examined under the same conditions (no contact with any other invertebrate). A. elegantissima tentacles had very low expression (0.2 [+ or -] 0.3 ng HSP70/[micro]g P) compared with the previous contact experiments. C. californica had high expression (2.1 [+ or -] 1.3 ng HSP70/[micro]g P) even when there was no direct (contact) aggression present. Comparing this analysis with the anemone-corallimorpharian experiments, no differences were found between HSP70 expressions in C. californica. There were differences in the HSP70 expression of polyps between the two cnidarians when they were compared together (F(1, 9) = 10.81, P < 0.0094).

The mean distance between competitors in field studies as determined from the photographs was 2.4 [+ or -] 0.9 mm (n 17). This distance is clearly within the range that A. elegantissima tentacle crowns sway during seawater movements (Francis, 1973a). The results of intraspecific competition in selected patches of both cnidarians are shown in Figure 3. There were clear differences in A. elegantissima HSP70 expression between the outside warrior polyps and the inside ones (in contact, 2.4 [+ or -] 0.5 ng HSP70/[micro]g P; no contact, 0.6 [+ or -] 0.7 ng HSP70/[micro]g P; F(3, 20) = 3.93, P < 0.0234, power of test = 0.82) when two clones of the same species interacted. Interestingly, C. californica had similar HSP70 amounts in polyps of different clones (outside 1.5 [+ or -] 1.1 ng HSP70/[micro]g P; inside 1.8 [+ or -] 1.3 ng HSP70/[micro]g P).

The regular cnidarian HSP70 expression in both outside and inside polyps of the clone in different competition-for-space situations is illustrated in Figure 4. A. elegantissima had more HSP70 in the warriors than in the inside clone polyps in general, depending on the competing species (Fig. 4). In Figure 5A, B we show HSP70 levels when both cnidarians interacted with the same competitors in the field: crustose red (Lithothamnium sp.) and fleshy green (Ulva sp.) algae. Contact with Lithothamnium (Fig. 5A) resulted in higher HSP70 expression in the outside A. elegantissima clone polyps (warriors, 2.4 [+ or -] 1.2 ng HSP/[micro]g P; inside ones 0.5 [+ or -] 0.4 ng HSP/[micro]g P, F(3, 10) 4.82, P < 0.025, power of test = 0.80). No differences were found between the inside and outside C. californica polyps in interactions with either algal species (outside 1.2 [+ or -] 0.4 ng HSP/[micro]g P; inside 1.5 [+ or -] 0.5 ng HSP/micro]g P).

Neither cnidarian showed any significant difference in HSP70 between inside and outside polyps (Fig. 5B). In A. elegantissima, the inside polyps (1.0 [+ or -] 0.8 ng HSP70/[micro]g P) were similar to the outside ones (0.6 [+ or -] 0.6 ng HSP70/[micro]g P). The expression was also similar for both clone polyps in C. californica (outside 1.2 [+ or -] 0.6 ng HSP70/[micro]g P; inside 1.3 [+ or -] 0.8 ng HSP70/[micro]g P). C. californica HSP70 expression was always the same in the outside and inside polyps (1-1.8 ng HSP70/[micro]g P) in encounters with either A. elegantissima, other C. californica clones, or either algal species.

For A. elegantissima, two intertidal competitors were tested in submersed conditions: A. xanthogrammica and Balanus amphitrite (Fig. 6A). Encounters with A. xanthogrammica resulted in higher HSP70 in A. elegantissima outside polyps (0.6 [+ or -] 0.2 ng HSP70/[micro]g P; inside ones 0.1 [+ or -] 0.1 ng HSP70/[micro]g P, F(3, 12) = 2.88, P < 0.048, power of test = 0.99). However, HSP70 levels were low compared with other situations (interactions with calcareous algae or other A. elegantissima clones). No differences in HSP70 level were found with the B. amphitrite interactions (outside 0.5 [+ or -] 0.6 ng HSP70/[micro]g P; inside 0.4 [+ or -] 0.4 ng HSP70/[micro]g P).

Differences in C. californica HSP70 levels occurred when potential encounters and fights for space were against the sponge Haliclona permollis or the ascidian Synoicum parfustis (Fig. 6B). HSP70 expression was the same in the outside and inside polyps, but was slightly higher than with other competitors. Both sponge and ascidian appear to activate higher HSP70 expression (H. permollis outside 3.1 [+ or -] 0.5 ng HSP70/[micro]g P; inside 2.5 [+ or -] 0.5 ng HSP70/[micro]g P; S. parfustis outside 2.4 [+ or -] 1.0 ng HSP70/[micro]g P; inside 1.8 [+ or -] 0.6 ng HSP70/[micro]g P). Again, no significant differences were found between inside and outside polyps. When comparing the response of this cnidarian against the sponge and the ascidian with all the other encounters, significant HSP70 differences were found (F(5, 79) = 18.58, P < 0.00001). HSP70 expression in the sponge and ascidian encounters was 2.2 [+ or -] 0.7 ng HSP70/[micro]g P, and in all the other encounters (A. elegantissima and C. californica, calcar eous and fleshy algae) the HSP70 level was 1.3 [+ or -] 0.6 ng HSP70/[micro]g P.

Discussion

Anthopleura elegantissima and Corynactis californica express HSP70 without physical stress (e.g., from temperature, desiccation, changes in pH) or pollution stress (e.g., due to heavy metals, organochlorines). There are few examples of cnidarian HSP expression patterns, and all are directly (Bosch et al., 1988; Bosch and Praetzel, 1991; Sharp et al., 1994) or indirectly (Hayes and King, 1995; Sharp et al., 1997; coral bleaching) related to temperature stress. This is the first set of observations relating aquatic invertebrate HSP levels to biological stress and relating cnidarian HSP expression to parameters other than temperature.

There were significant differences in HSP70 levels between the two cnidarians, and these depended on the particular competing species. Perhaps the aggressive behavior of C. californica (Chadwick, 1987, 1991; Chadwick and Adams, 1991) causes cellular damage, thereby increasing HSP70 expression levels in A. elegantissima tentacles (Fig. 2) in the first aquarium experiments. C. ca1ifornica extrudes mesentarial filaments upon contact with nonfood species, suggesting that this behavior is used in interspecies aggressive encounters (Chadwick, 1987; Chadwick and Adams, 1991). Prolonged contact with C. californica mesentarial filaments kills the competitor. In this forced situation, no stresses other than contact between polyps appear to affect the tentacles of both cnidarians. In comparison with isolated (non-interacting) A. elegantissima polyps (Fig. 2), the expression of HSP70 is nearly 20 times greater after 48 h of interspecific interactions. The differences shown between tentacle crown and whole body in A. eleg antissima were not found in C. californica.

The more striking result is the lack of differences between the solitary and interacting C. californica polyps in the aquarium experiences (in Fig. 2, compare 24 and 48 h). The expression of HSP70 is high and very constant in the three interspecific encounters (1.3-2.1 ng HSP70/[micro]g P). One explanation could be that the aggressive behavior of some corallimorpharians requires cellular protection to counter the effect of the competing species' response (Chadwick, 1987; Langmead and Chadwick, 1999a, b). After a period of contact with C. californica, A. elegantissima moved away via pedal locomotion, suggesting that the specialized aggressive structures of the anemone were ineffective against the corallimorpharian (Francis, 1973a, b; Chadwick, 1987).

Strong intraspecific competition has been clearly demonstrated between clones of A. elegantissima (Francis, 1973a, b; Ayre and Grossberg, 1995, 1996). Contact between genetically different individuals of this species initiates elaborate behaviors involving acrorhagial contact (leaving patches of tissue containing high numbers of nematocysts) and results in damage to one or both competitors. In addition, anemones of the genus Anthopleura, including A. xanthogrammica (discussed below), produce cytolytic and sodium-channel toxins that presumably damage cellular constituents such as proteins following contact (Bernheimer and Lai, 1985; Cline and Wolowyk, 1997; Kelso and Blumenthal, 1998). These toxic mechanisms could explain the high HSP70 levels found in the examined clones (Fig. 3). The outside warrior polyps bordering neighboring clones have more HSP70 than the inside ones. Sessile organisms discontinuously fight for space, depending on growth and reproductive cycles, the age of competitors, or the nature of t he enemies (Connell, 1961; Jackson, 1977; Chadwick, 1991). Perhaps when warrior polyps encounter a "known" competitor (i.e., in this case a different clone of the same species), they become "prepared for war," producing HSP70 levels high enough to avoid serious cellular damage when real interactions begin. Alternatively, some interactions have already caused some tissue damage, resulting in higher HSP70.

No differences in HSP70 expression were expected in interactions between A. elegantissima and a fleshy green alga (Ulva sp., Fig. SB). This algal type escapes from direct competition for space by growing as rapidly as nutrients and light levels permit (Lewis, 1964; Paine, 1971). No direct interactions were evident, and the low HSP70 levels found in the outside interacting polyps of these clones seem to confirm their absence, although algae in this genus are capable of producing harmful secondary compounds (Paine, 1990; Whitfield et al., 1999). In the case of Lithothamnium sp. (Fig. 5A), it is known that coralline algae grow slowly (Steneck, 1986; Garrabou and Ballesteros, 2000) and can synthesize allelochemicals (as do some other red algae) to compete for space (Whitfield et al., 1999). Perhaps the anemone better detects or is more affected by these Lithothamniwn chemicals than by those produced by Ulva.

A. xanthogrammica is a common intertidal competitor with A. elegantissima for space (Francis, 1973b; Sebens, 1984). This solitary anemone elicits aggression in A. elegantissima (Francis, 1973b) but does not display the same mechanisms of defense. Observations made by Sebens (1984) support the idea that aggression is common between these two species, which explains the higher levels of HSP70 in the outside A. elegantissima polyps in these interactions (Fig. 6A). Balanus amphitrite, another common space competitor, seems to have no effect on HSP70 expression (Fig. 6A). It is possible that the lack of effect was due to exposure to small individual cirripeds, and it would be interesting to examine A. elegantissima clones that are in competition for space with larger clumps of barnacles.

In C. californica, HSP70 levels are similar in outside and inside clone polyps. Therefore the corallimorpharian does not distinguish between the exposed (outside polyps) and nonexposed (inside polyps) areas of the clone. More importantly, even without apparent interactions (Fig. 2), C. californica expresses HSP7O at constant levels (1-2 ng HSP70/[micro]g P). In this species, intraspecific competition results in HSP70 levels that are within the "normal" range (Fig. 3), and there is no aggressive behavior in intraspecific contacts (Chadwick, 1987). Perhaps the key to interpreting HSP70 expression as a mechanism of competence in C. californica is the finding that the highest HSP70 levels were found in polyps interacting with Haliclona or Synoicum (Fig. 6B). Also of importance is that these differences between interacting and non-interacting polyps were significant. It is known that sponges and ascidians use chemical substances to defend themselves or attack potential foes competing for substrata (Green, 1977; Su chanek et al., 1985; Thompson et al., 1985; Turon et al., 1996, 1998; Becerro et al., 1997). We suggest that HSP70 expression differences found when the encounter involves ascidians or sponges may reflect the aggressive toxic substances used by these enemies (Uriz et al., 1991).

C. californica appears to be always "prepared for war" by its aggressive behavior (Chadwick, 1991). Another organism that exhibits this strategic use of stress proteins (by maintaining a basal level of HSP expression) is the desert-dwelling ant Cataglyphys. This ant presynthesizes HSPs at relatively low nest temperatures to limit damage from heat shock on the desert floor. Coupled with continued HSP production at higher temperatures, this protects the ant from the high temperatures it experiences when foraging in daytime (Gehring and Wehner, 1995). Perhaps the presynthesis of HSP70 in C. californica provides protection from neighbors that intermittently excrete harmful substances. Alternatively, the constant HSP70 levels might protect the corallimorpharian against its own aggressive substances, which it uses to catch prey and to fight for space (Chadwick, 1987). The aggressive behavior of C. californica includes the extrusion of mesenteric filaments containing gland cells that secrete strong proteolytic enzym es and nematocysts that may inject cytolytic toxins into prey or enemies (Van-Praet, 1985).

Because of the high cost of the HSP expression and its occasional harmful effect if constantly highly expressed (Feder et al., 1992; Krebs and Feder, 1997), we suggest that expression varies depending on the kind of neighboring competitor or enemy. Furthermore, A. elegantissima also expresses high levels of HSP70 in response to physical factors, especially temperature (Rossi and Snyder, unpubl. obs.). The anemone has to "share" HSP70 expression between biological (e.g., competition for space) and physical (e.g., temperature) factors.

It is also possible that other stress proteins contribute to the responses against biological phenomena such as competitive interactions for space in the benthic environment. For example, unexpected low-molecular-weight HSP7O homologs have been found in other cnidarians (Sharp et al., 1994). HSP60 has known roles in thermal acclimation of the cnidarians Hydra vulgaris and Acropora grandis (Bosch et al., 1988; Fang et al., 1997). The use of SPA-822 HSP70 antiserum can possibly underestimate the number of HSP70 isoforms, and consequently may explain the finding of single HSP70 proteins by our methods. However, we have successfully used the same antiserum and measured two and three to four different HSP70 isoforms in larval lobsters, (Homarus americanus), and juvenile abalone (Haliotis rufescens) and adult mussels (Mytilus galloprovincialis) respectively (Snyder and Mulder, 2001; Snyder et al., 2001).

Many questions remain unanswered, such as the identity of the harmful substances or aggressive behaviors that activate HSP7O expression in competitive interactions among sessile marine invertebrates. Among the likely candidates for cellular damaging allelochemicals are cnidarian sodium channel toxins (Kelso and Blumenthal, 1998), cytotoxic and cytolytic factors (Bernheimer and Lai, 1985; Cline and Wolowyk, 1997), and an array of toxic alkaloids found in cnidarians and sponges (e.g., Djura and Faulkner, 1980; Koh and Sweatman, 2000). Such chemicals can diffuse and act at some distance from the source or can be deposited on neighboring organisms by direct contact (e.g., Schmitt et al., 1995; Slattery et al., 1997). Further studies of HSP proteins may provide important information about the consequent distribution and hierarchy of species in the rocky benthos.

With this work we propose HSP70 expression as a tool for evaluating space competition among sessile marine invertebrates, without manipulative experiments. From our results, it is clear that the expression of the stress proteins depends on both the particular competing species and the interacting life stages of each competitor. The energy required to repair tissue damage cannot be used for other processes such as reproduction and growth. It will be interesting to measure how the amount of energy an organism devotes to growth and reproduction varies with the level of HSP produced during prolonged competition for space.

Acknowledgments

The manuscript was improved by the comments of Drs. Josep-Maria Gili, Cadet Hand, and several anonymous reviewers. This work was supported by the National Sea Grant College Program, National Oceanic and Atmospheric Administration, U.S. Department of Commerce, under grant number NA66RG0477, project number R/A-108, through the California Sea Grant College Program and an F.P.I. fellowship from "Ministerio de Educacion y Ciencia" to S.R. through the DGICYT grants PB94-0014-C02-01 and PB98-0496-C03-01. The views expressed herein are those of the authors and do not necessarily reflect the views of NOAA or any of its sub-agencies. The U.S. Government is authorized to reproduce and distribute this publication for governmental purposes. Contribution 2136 from the Bodega Marine Laboratory, University of California at Davis.

Received 26 January 2001; accepted 22 May 2001.

(*.) Current address: Institut de Ciences del Mar. Passeig Nacional, s/n. 08003 Barcelona, Spain.

(+.) To whom correspondence should be addressed. E-mail: mjsnyder@ucdavis.edu

Literature Cited

Ayre, D. J., and R. K. Grosberg. 1995. Aggression, habituation, and clonal coexistence in the sea anemone Anthopleura elegantissima. Am. Nat. 146: 427-453.

Ayre, D. J., and R. K. Grosberg. 1996. Effects of social organization on inter-clonal dominance relationships in the sea anemone Anthopleura elegantissima. Anim. Behav. 51: 1233-1245.

Becerro, M. A., X. Turon, and M. J. Uriz. 1997. Multiple functions for the secondary metabolites in encrusting marine invertebrates. J. Chem. Ecol. 23: 1527-1547.

Bernheimer, A. W., and C. Y. Lai. 1985. Properties of a cytolytic toxin from the sea anemone, Stoichacyis kenti. Toxicon 23: 791-800.

Bosch, T. C. G., and G. Praetzel. 1991. The heat shock response in Hydra: immunological relationships of hsp60, the major heat shock protein of Hydra vulgaris, to the ubiquitous hsp70 family. Hydrobiologia 216/217: 513-517.

Bosch, T. C. G., S. M. Krylow, H. R. Bode, and R. E. Steele. 1988. Thermotolerance and synthesis of heat shock proteins; these responses are present in Hydra attenuata but absent in Hydra oligactis. Proc. Natl. Acad. Sci. USA 85: 7927-7931.

Chadwick, N. E. 1987. Interspecific aggressive behavior of the corallimorpharian Corynactis californica (Cnidaria: Anthozoa): effects on sympatric corals and sea anemones. Biol. Bull. 173: 110-125.

Chadwick, N. E. 1991. Spatial distribution and the effects of competition on some temperate Scleractinia and Corallimorpharia. Mar. Ecol. Prog. Ser. 70: 39-48.

Chadwick, N. E., and C. Adams. 1991. Locomotion, asexual reproduction and the killing of corals by the corallimorpharian Corynactis californica. Hydrabialagia 216/217: 263-269.

Chornesky, E. A. 1983. Induced development of sweeper tentacles on the reef coral Agaricia agaricites: a response to direct competition. Biol. Bull. 165: 569-581.

Cline, E. I., and M. W. Wolowyk. 1997. Cardiac stimulatory, cytotoxic and cytolytic activity of extracts of sea anemones. Int. J. Pharmacogn. 35: 91-98.

Connell, J. H. 1961. The influence of interspecific competition and other factors on the distribution of the barnacle Chthamalus stellatus. Ecology 42: 315-328.

Dayton, P. K. 1971. Competition, disturbance, and community organization: the provision and subsequent utilization of space in a rocky intertidal community. Ecol. Monogr. 41: 351-389.

Djura, P., and D. J. Faulkner. 1980. Metabolites of the marine sponge Dercitus sp. J. Org. Chem. 45: 735-737.

Fang, L.-s., S.-p. Huang, and K.-1 Lin. 1997. High temperature induces the synthesis of heat-shock proteins and the elevation of intracellular calcium in the coral Acropora grandis. Coral Reefs 16: 127-131.

Feder, M. E., and G. E. Hofmann. 1999. Heat-shock proteins, molecular chaperones, and the stress response: evolutionary and ecological physiology. Annu. Rev. Physiol. 61: 243-282.

Feder, M. E., J. M. Rossi, J. Solomon, N. Solomon, and S. Lindquist. 1992. The consequences of expressing hsp70 in Drosophila cells at normal temperatures. Genes Dev. 6: 1402-1413.

Fitt, W. K., R. L. Pardy, and M. M. Littler. 1982. Photosynthesis, respiration, and contribution to community productivity of the symbiotic sea anemone Anthopleura elegantissima (Brandt, 1835). J. Exp. Mar. Biol. Ecol. 61: 213-232.

Francis, L. 1973a. Clone specific segregation in the sea anemone Anthopleura elegantissima. Biol. Bull. 144: 64-72.

Francis, L. 1973b. Intraspecific aggression and its effect on the distribution of Anthopleura elegantissima and some related sea anemones. Biol. Bull. 144: 73-92.

Francis, L. 1976. Social organization within clones of the sea anemone Anthopleura elegantissima. Biol. Bull. 150: 361-376.

Garrabou, J., and J. Ballesteros. 2000. Growth of Mesophyllum alternans and Lithophyllum frondosum (Corallines, Rhodophyta) in the northwestern Mediterranean. Eur. J. Phycol. 35: 1-10.

Gehring, W. J., and R. Wehner. 1995. Heat shock protein synthesis and thermotolerance in Cataglyphis, an ant from the Sahara desert. Proc. Natl. Acad. Sci. USA 92: 2994-2998.

Green, G. 1977. Ecology of toxicity in marine sponges. Mar. Biol. 40: 207-215.

Hayes, R. L., and C. M. King. 1995. Induction of 70-kD heat shock protein in scleractinian corals by elevated temperature: significance for coral bleaching. Mol. Mar. Biol. Biotechnol. 4: 36-42.

Ireland, C. M., D. M. Roll, T. F. Molinski, T. C. McKee, T. M. Zabriskie, and J. C. Swersey. 1988. Uniqueness of the marine chemical environment: categories of marine natural products from invertebrates. Biomedical importance of marine organisms. Mem. Calif Acad. Sci. 13: 41-57.

Jackson, J. B. C. 1977. Competition on marine hard substrata: the adaptive significance of solitary and colonial strategies. Am. Nat. 111: 743-767.

Kelso, G. J., and K. M. Blumenthal. 1998. Identification and characterization of novel sodium channel toxins from the sea anemone Anthopleura xanthogrammica. Toxicon 36: 41-51.

Koh, E. G. L., and H. Sweatman. 2000. Chemical warfare among scleractinians: bioactive natural products from Tubastraea faulkneri Wells kill larvae of potential competitors. J. Exp. Mar. Biol. Ecol. 251: 141-160.

Krebs, R., and M. E. Feder. 1997. Deleterious consequences of Hsp70 overexpression in Drosophila melanogaster larvae. Cell Stress Chaperanes 2: 60-71.

Laemmli, U. K. 1970. The cleavage of structural proteins during assembly of the head of bacteriophage T4. Nature 227: 680-685.

Langmead, O., and N. E. Chadwick. 1999a. Marginal tentacles of the corallimorpharian Rhodactis rhodostoma. 1. Role in competition for space. Mar. Biol. 134: 479-489.

Langmead, O., and N. E. Chadwick. 1999b. Marginal tentacles of the corallimorpharian Rhodactis rhodostoma. 2. Induced development and long-term effects on coral competitors. Mar. Biol. 134: 491-500.

Lewis, J. R. 1964. The Ecology of Rocky Shores. English Universities Press, London.

McCain, B. B., D. W. Brown, M. M. Krahn, M. S. Myers, R. C. Clark, S.-L. Chan, and D. C. Malins. 1988. Marine pollution problems, North American West Coast. Aquat. Toxicol. 11: 143-162.

Paine, R. T. 1971. A short-term experimental investigation of resource partitioning in a New Zealand rocky intertidal habitat. Ecology 52: 1096-1106.

Paine, R. T. 1990. Benthic macroalgal competition: complications and consequences. J. Phycol. 26: 12-17.

Pequegnat, W. E. 1964. The epifauna of a California siltstone reef. Ecology 45: 272-283.

Roberts, D. A., G. E. Hofmann, and G. N. Somero. 1997. Heat shock protein expression in Mytilus californianus: acclimatization (seasonal and tidal-height comparisons) and acclimation effects. Biol. Bull. 192: 309-320.

Schmitt, T. M., M. E. Hay, and N. Lindquist. 1995. Constraints on chemically mediated coevolution: multiple functions for seaweed secondary metabolites. Ecology 76: 107-123.

Schoener, T. W. 1983. Field experiments on interspecific competition. Am. Nat, 122: 240-285.

Sebens, K. P. 1982a. The limits to indeterminate growth: an optimal size model applied to passive suspension feeders. Ecology 63: 209-222.

Sebens, K. P. 1982b. Asexual reproduction in Anthopleura elegantissima (Anthozoa:Actinaria): seasonality and spatial extent of clones. Ecology 63: 434-444.

Sebens, K. P. 1984. Agonistic behavior in the intertidal sea anemone Anthopleura xanthogrammica. Biol. Bull. 166: 457-472.

Sharp, V. A., D. Miller, J. C. Bythell, and B. E. Brown. 1994. Expression of low molecular weight HSP 70 related polypeptides from the symbiotic sea anemone Anemonia viridis Forskall in response to heat shock. J. Exp. Mar. Biol. Ecol. 179: 179-193.

Sharp, V. A., B. E. Brown, and D. Miller. 1997. Heat shock protein (HSP70) expression in the tropical reef coral Goniopora djiboutiensis. J. Therm. Biol. 22: 11-19.

Slattery, M., M. T. Hamann, J. B. MeClintock, T. L. Perry, M. P. Puglisi, and W. Y. Yoshida. 1997. Ecological roles for water-borne metabolites from Antarctic soft corals. Mar. Ecol. Prog. Ser. 161: 133-144.

Snyder, M. J., and E. P. Mulder. 2001. Environmental endocrine disruption in decapod crustacean larvae: Hormone titers, cytochrome P450, and stress proteins. Aquat Toxicol. 55: 177-190.

Snyder, M. J., E. Girvetz, and E. P. Mulder. 2001. Stress protein induction by chemical exposures in molluscs. Arch. Environ. Contam. Toxieol. 41: 22-29.

Steneck, R. S. 1986. The ecology of coralline algal crusts: convergent patterns and adaptative strategies. Annu. Rev. Ecol. Syst. 17: 273-303.

Suchanek, T. H., R. C. Carpenter, J. D. Witman, and C. D. Harvell. 1985. Sponges as important space competitors in deep Caribbean coral reef communities. Pp. 55-59 in The Ecology of Deep and Shallow Coral Reefs, M. L. Reaka, ed. Symposia Series for Undersea Research 3(1), NOAA/NURP, Rockville, MD.

Sutherland, J. P. 1978. Functional roles of Schizoporella and Styela in the fouling community at Beaufort, North Carolina. Ecology 59: 257-264.

Thompson, J. E., R. P. Walker, and D. J. Faulkner. 1985. Screening and bioassays for biologically-active substances from forty marine sponge species from San Diego, California USA. Mar. Biol. 88: 11-21.

Turon, X., M. A. Becerro, M. J. Uriz, and 3. Llopis. 1996. Small-scale associations measures in epibenthic communities as a clue for allelochemical interactions. Oecologia 108: 35 1-360.

Turon, X., I Tarjuelo, and M. J. Uriz. 1998. Growth dynamics and mortality of the encrusting sponge Crambe crambe (Poecilosclerida) in contrasting habitats: correlation with population structure and investment in defense. Funct. Ecol. 12: 63 1-639.

Uriz, M. J., D. Martin, X. Turon, E. Ballesteros, R. Hughes, and C. Acebal. 1991. An approach to the ecological significance of chemically mediated bioactivity in Mediterranean benthic communities. Mar. Ecol. Prog. Ser. 70: 175-188.

Van-Praet, M. 1985. Nutrition of sea anemones. Adv. Mar. Biol. 22: 65-99.

Weis, V. M., and R. P. Levine. 1996. Differential protein profiles reflect the different lifestyles of symbiotic and aposymbiotic Anthopleura elegantissima, a sea anemone from temperate waters. J. Exp. Biol. 199: 883-892.

Whitfield, F. B., F. Helidoniotis, K. J. Shaw, and D. Svoronos. 1999. Distribution of bromophenols in species of marine algae from eastern Australia. J. Agric. Food Chem. 47: 2367-2373.

Whittaker, R. H., and P. P. Feeny. 1973. Allelochemics: chemical interaction between species. Science 171: 757-768.

Wiens, M., C. Koziol, H. M. A. Hassanein, R. Batel, H. C. Schroder, and W. E. G. Muller. 1998. Induction of gene expression of the chaperones 14-3-3 and HSP70 by PCB 118 (2,3', 4,4', 5-pentachlorobiphenyl) in the marine sponge Geodia cydonium: novel biomarkers for ploychlorinated biphenyls. Mar. Ecol. Prog. Ser. 165: 247-257.

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Author:Rossi, Sergi; Snyder, Mark J.
Publication:The Biological Bulletin
Geographic Code:1USA
Date:Dec 1, 2001
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