Comparison of bleeding time and clotting time between males and females.
Bleeding time (BT) can be defined as the time taken from the puncture of the blood vessel to the stoppage of the bleeding. Bleeding normally lasts for 2-6 min. BT and clotting time (CT) are done for blood transfusion for various purposes and to diagnose various disorders of functions of platelets and clotting factors.
BT depends on various factors such as functions of platelets and endothelial cells of arteries and pathways of coagulation.[3-5]
CT is the time interval from puncture of blood vessel to the formation of fibrin thread. Normal value of CT is 3-8 min. CT is increased due to the absence or abnormality of clotting factors. CT is higher in females as compared to males.  This is because of increased estrogen in females which prolongs CT and decreases plasma fibrinogen level. BT is increased in females due to the presence of estrogens which, in turn, reduce the functions of platelets.[10,11] BT is decreased in males due to increased activation and aggregation of platelets.[12-16] The major focus of the present investigation is to demonstrate the gender wise differences in BT and CT.
MATERIALS AND METHODS
The study was done at the Department of Physiology, TMMC and RC over a period of 12 months. A group of 200 volunteers from the western U.P population was taken for the study. Of 200 volunteers, 84 were male and 116 were female. Approval for the study was taken by the institutional ethical committee.
* Males and females between the ages of 18 and 30 years.
The following criteria are excluded from the study:
* Those without written informed consent.
* History of any bleeding and clotting disorder like purpura.
* History of any medication causing abnormal BT and CT like NSAIDs.
The BT was determined by Duke's method using Whatman filter paper. CT was determined by capillary tube method.
The data were collected and analyzed using SPSS (version 23).
The comparison of mean BT and CT between males and females was done using unpaired t-test.
Of 200 volunteers, 84 were male and 116 were female. The comparison of mean BT and CT between males and females was done using unpaired t-test. It was found that the mean BT and CT in males were 128.69, 44 and 219.88 respectively. The mean BT and CT in females were 133.28 and 223.97, respectively. The mean BT (P = 0.047) and CT (P = 0.046) were significantly more in females as compared to males [Table 1].
The mean BT (P = 0.047) and CT (P = 0.046) were more in females as compared to males and the difference was statistically significant.
Aleemet et al. studied correlation of blood groups, BT, and CT in male and female students. They conducted a study on 122 students, of which 35 were male and 87 were female. Similar to our result, they found that BT and CT were slightly more in females as compared to males. Reeta studied gender wise relation of BT and CT, of 154 students, 59 were female and 95 were male. They also found that BT and CT were higher in females as compared to males. Roy et al. conducted a study in 261 volunteers; they also found higher BT and CT in females as compared to males. Meena and Sunil conducted a study on 150 students. They also found that BT and CT were higher in females than males. Manjeet and Arvinder conducted a study on 150 students, they found that BT and CT were higher in females than males, but the differences were statistically not significant.The comparison of BT and CT between males and females shows that females have greater values of BT and CT as compared to males; it is attributed mainly to hormonal difference between males and females. Females are having higher levels of estrogen and lower levels of fibrinogen in blood plasma as compared to males. The estrogen decreases the plasma level of fibrinogen and increases the CT. It causes dilatation of blood vessels so prolongs the BT.
Duke's method for BT and capillary tube method for CT though easy and inexpensive methods, but they are not very accurate methods.
The future studies can be conducted using other test methods such as estimation of prothrombin time, activated partial thromboplastin time, study of platelet aggregation, and determination of coagulation factor.
It can be concluded that BT and CT are higher in females as compared to males and the difference is statistically significant.
[1.] Bijlani RL, Manjunatha S. Understanding Medical Physiology. 4th ed. India: Jaypee Publication; 2011.
[2.] Roy B, Banerjee I, Sathian B, mondal M, Saha CG. Blood group distribution and its relationship with bleeding time and clotting time: A medical school based observational study among Nepali. Indian and Sri Lankan students. Nepal J Epidemiol 2011;1:135-40.
[3.] Born GVR. Platelets and blood vessels. J Cardiovasc Pharm 1984;6:S706-13.
[4.] Kristensen SD, Schmidt EB, Dyerberg J. Dietary supplementation with n-3 polyunsaturated fatty acids and human platelet function: A review with particular emphasis on implications for cardiovascular disease. J Int Med 1989;22 Suppl1:141-50.
[5.] Markovitz JH. Hostility is associated with increased platelet activation in coronary heart disease. Psychosom Med 1998;60:586-91.
[6.] Guyton JH, Hall J. Text Book for Medical Physiology. 11th ed. Philadelphia, PA: Elsevier Saunders; 1956. p. 467.
[7.] Pal GK, Pravati PA. Text Book of Medical Physiology. 2nd ed. India: Ahuja Publishing House; 2007. p. 148.
[8.] Ercan M, Yegin E, Akdeniz H, Irmak H, Bayiroglu F, Tuncer I. Effect of estrogen on fibrinogen clotting time in rabbits. Turkish J Vet Anim Sci 1998;22:137-40.
[9.] Jacob B, Ron T, Jacob F, Yoseph P, Shmuel G, Jardena O. The effect of estrogen replacement therapy on platelet aggregation and adenosine tri phosphate release in postmenopausal women. Obstet Gynecol 1993;81:261-4.
[10.] Mller VM, Jayachandran M, Hashimoto K, Heit JA, Owen WG. Estrogen, inflammation and platelet phenotype. Gend Med 2008;5 Suppl A: S91-102.
[11.] Pitney WR, Nicol M, Dean S, Hickey A. Efect of flurbiprofen on bleeding time and platelet aggregation. Thromb Res 1978;13:811-9.
[12.] Miner PC, Martin JF. Shortened bleeding time in acute myocardial infarction and its relation to platelet mass Brit Med J 1985;290:1767-70.
[13.] Kristensen SD, Bath PM, Martin JF. Differences in bleeding time, aspirin sensitivity and adrenaline between acute myocardial infarction and unstable angina. Cardiovasc Res 1990;24:19-23.
[14.] Harrison P, Mackie I, Mathur A, Robinson MS, Hong Y, Erusalimsky JD, et al. Platelet hyperfunction in acute coronary syndromes. Blood Coagul Fibrinolysis 2005;16:557-62.
[15.] O'Brien JR, Jamieson S, Etherington M, Klaber MR, Ainsworth JF. Stressed template bleeding time and other platelet-function tests in myocardial infarction. Lancet 1973;301:694-6.
[16.] Martina E. Daly. Determinants of platelet count in humans. Haematologica 2011;96:10-3.
[17.] Mahapatra B, Mshra N. Comparison of bleeding time and clotting time in different blood groups. Am J Infect Dis 2009;5:106-8.
[18.] Ghai CL. A Text of Practical Physiology. 8th ed. New Delhi: Jaypee Brothers; 2013. p. 113-4.
[19.] Ambreen A, Muqeet W. Correlation of blood groups, bleeding time and clotting time in male and female students; An observational study. Pak J Pharm Res 2016;2:121-6.
[20.] Reeta B. Blood group and its relationship with bleeding time and clotting time-an observational study among the 1st MBBS students of Gauhati medical college. Int J Res Med Sci 2017;5:4147-50.
[21.] Meena M, Sunil KJ. Distribution of blood group and its relation to bleeding time and clotting time. Int J Med Sci Public Health 2016;5:1-4.
[22.] Manjeet K, Arvinder S. Blood group distribution and its relationship with bleeding time and clotting time. Natl J of Physiol Pharmacol 2015;5:253-7.
Ritu Adhana, Rajanish Chaurasiya, Anjali Verma
Department of Physiology, Teerthanker Mahaveer Medical College and Research Centre, Moradabad, Uttar Pradesh, India
Correspondence to: Ritu Adhana, E-mail: firstname.lastname@example.org
Received: June 02, 2018; Accepted: June 17, 2018
How to cite this article: Adhana R, Chaurasiya R, Verma A. Comparison of bleeding time and clotting time between males and females. Natl J Physiol Pharm Pharmacol 2018;8(10):1388-1390.
Source of Support: Nil, Conflict of Interest: None declared.
Table 1: The comparison of mean BT and CT between males and females (n=200) Parameters Mean[+ or -]SD Mean difference Male Female BT (s) 128.69[+ or -]44.09 133.28[+ or -]44.33 -4.59 CT (s) 219.88[+ or -]50.50 223.97[+ or -]57.92 -4.09 Parameters Mean difference t-test value P BT (s) -4.59 -3.724 0.047 (*) CT (s) -4.09 -3.519 0.046 (*) (*) P<0.05 (significant); ^>0.05 (not significant). BT: Bleeding time, CT: Clotting time
|Printer friendly Cite/link Email Feedback|
|Title Annotation:||RESEARCH ARTICLE|
|Author:||Adhana, Ritu; Chaurasiya, Rajanish; Verma, Anjali|
|Publication:||National Journal of Physiology, Pharmacy and Pharmacology|
|Date:||Oct 1, 2018|
|Previous Article:||Human constitutional types of Ayurveda and its relation with hematological parameters in infants.|
|Next Article:||Association of ABO blood group with different constitutional types.|