Comparative study of four hematology analyzers.
However, quite a few pathologists are satisfied with whatever results their counter gives them without realizing the importance of regular checks and quality control exercises for obtaining reliable results. A number of analyzers of different makes are available in the market today. Most of these counters are pre-calibrated by the company in certain modes; however, there are certain parameters which can be calibrated by the individual pathologist. As a result there is bound to be some degree of variability in the results obtained from each counter.
Yet no counter can be called good or bad. The "goodness" or "badness" of a counter is largely created by the operator. Depending on his keen observation and recognition that his counter is going wrong in a particular aspect (which can then be recalibrated), the operator can maintain his counter in good shape. Here is where a need for Inter-laboratory Quality Control programs (External Quality Assessment) is felt necessary. Same blood sample(s) may be run at different laboratories and the variability in the results obtained may be studied and gross variations may then be analyzed and corrected accordingly, on the basis of standard protocols.
A rather easy and fairly reliable method of carrying out quality control exercises is with the help of commercially available cell controls. The cell controls are run on instruments and if the results obtained do not fall within the expected range, the cell counter may be recalibrated.
The Complete Blood Count (CBC) is the most frequently performed investigation by a laboratory, be it private or institutional, clinical or research-oriented. In effect, up to 70% of the workload of a laboratory is comprised of CBCs prescribed by general practitioners, internists, hematologists and other super specialists. The OPD laboratory as well as indoor hematology laboratory including sickle cell laboratory at New Civil Hospital, Surat is well equipped with the facility of automated cell counters.
Most of the counters are of different makes. After having seen a number of CBC reports of individual patients and an occasional CBC report of the same patient from two laboratories, a definite need was felt to find out the reliability and comparability of cell counters being used at different laboratories in new civil hospital Surat. Blood counts now being the basic investigations and routine procedure before any further workup of patient should be dealt with caution as analyzers are routinely being used for them. Fully automated instruments require only an appropriate amount of blood sample that is presented to the instrument. They are multichannel instruments, i.e. they usually measure 8-20 variables including some new parameters which do not have any equivalent in manual techniques (1).
Automation in hematology has gained wide acceptance in India. There are various makes of automated hematology analyzers available. Whether all of these are similar in their results or not, this study is an attempt to assess the same with available set of analyzers. Prerequisite before judging these counters was that they were running within control by their respective company manufactured control samples. As well as all of them were calibrated and two out of four were under EQAP program assessment.
MATERIAL AND METHODS: This study was conducted at Pathology department Govt. medical college, Surat. Random selection by selecting every fourth patient's sample for running on analyzer (whether male or female) was done. Four automated hematology analyzers of different make were used. Sample was run within two hours of collection. Hematology analyzers were ABX-Micros-60, Sysmex-KX-21, Advia-120 and Erma-PCE-210. Total ten samples of EQAP and 62 patient's sample were run on all the counters. On Advia-120 only one EQAP sample was run as it was introduced late in the study.
All machines were under strict quality check and were calibrated. EQAP results of the two machines i.e. sysmex-KX-21 and ABX-Micros-60 were satisfactory throughout the test. Since these two machines were under NABL scope throughout the test one of these two machines i.e. sysmex-KX21 was selected for the inter-instrument comparison. These two machines were having good linearity, no carry over and good precision. Range for linearity was higher for sysmex-KX-21 for all counts hence it was taken as standard for inter-instrument comparison.
This study included one five part analyzer Advia-120 so parameters considered for comparison were hemoglobin, RBC counts, WBC counts, platelet counts, hematocrit, MCV and lymphocyte percentage. Overall assessment was to check whether all analyzers if undergoing strict quality check, give similar results (statistically) or they differ.
ETHICS: Samples collected for checking were original samples derived from the patients. EQAP samples came from the AIIMS, New Delhi.
STATISTICS: Modified-ANOVA (Analysis of Variance) was used.
DISCUSSION: All pathologists want an analyzer that gives the best result. The hematology analyzers which are under strict quality check specially when they are graded as satisfactory on EQAP assessment need not necessary give similar results when tested with a different set of quantitative data (i.e. patient's with different clinical conditions with varied hematological results).
Most of the studies mentioned in literature have evaluated newer counters in comparison to the better known, established cell counters. Two studies which carried out a parallel evaluation of more than two counters those conducted by Jones et al (1995) (2) and Bentley et al (1993) (3) respectively. Recent study by Drewinko B, comparing flow cytometry based analyzers with the conventional ones showed their superiority in terms of linearity and precision (4), but how much impact does it make in assessment was included in our study as one analyzer Advia120 is based on flow cytometry in our study.
Jones et al (2) compared the performance of cell counters by analyzing the coefficient of correlation. Many other workers have also evaluated counters in terms of coefficient of correlation. However, J M England (1996) (5) comments that correlation coefficients should not be used to analyze data while evaluating a cell counter since they give no information about comparability. As many as 20% of the values may be found to fall out of line for a comparison which has otherwise been reported by a correlation coefficient of 1.
In previous two studies by Bain et al on comparison between two cell counters one study showed with respect to accuracy both automated counters showed statistically significant difference from each other in differential counts (6). Other study showed 58% of blood samples counted by one cell counter required a blood film examination, and other one was imprecise in results (7). One study by Devreese et al showed two analyzers to be correlating good for hemoglobin, WBC counts, hematocrit and platelet counts (8). And similar was the finding of the study conducted by Sheridan et al on two different make analyzers (9).
In the west, it is mandatory for laboratories to participate in the external quality assessment programmes e.g. in the United Kingdom, for a laboratory to get accreditation it has to participate in the UK NEQAS (H) (United Kingdom National Quality Assessment for Hematology) (5). Similarly in the USA as per Clinical Laboratory Amendment 1988 (CLIA, 1988) 5 laboratories have to participate in external quality assessment programmes such as those being run by the College of American Pathologists. In India, this is done by All India Institute of Medical Sciences (AIIMS) through program called EQAP (10).
As parallel part of study the EQAP samples were run on ABX-Micros-60, Sysmex-KX-21 and Erma-PCE-210. As is evident from tables all counters reported different mean values for each variable in first phase of the study which dealt with random patient samples. The variability of the calculated means of all variables was analyzed by deriving the coefficient of variation (CV). A difference in the CV was observed but Sysmex-KX-21 and Advia-120 showed nearly same CV for all seven parameters (Table 1, 3, 5, 7, 9, 11, 13). The lowest and highest CV was for MCV (mean corpuscular volume) (Table 13, 14) and total leukocyte counts (Table 5, 6) respectively.
Since machines were calibrated and EQAP results applied to Sysmex-KX-21 and ABX-Micros60 (both under EQAP program separately) and stamped as satisfactory by results of EQAP, the difference that come as a result of patients samples run on them indicated inherent system capacity (difference).
CONCLUSION: As per statistical conclusion for total WBC & RBC counts (Table 5, 6, 7, 8) all the counters showed nearly same values and they don't differ. For platelet counts (Table 1, 2) SysmexKX-21 and Advia-120 are similar rest all differ. For hematocrit (Table 9, 10) Sysmex-KX-21, Advia 120, PCE-210 are same but ABX-Micros-60 differ. For MCV (Table 13, 14) and lymphocyte percentage (Table 11, 12) Sysmex-KX-21 and PCE-210 are same, rest all differ. For Hemoglobin (Table 3, 4) all counters are different.
(1.) Bain BJ Bates I. Basic haematological techniques. Lewis SM, Bain BJ Bates I(ed). Dacie and Lewis Practical Hematology, 18th Edition. London: Churchill Livingstone; 2001; 19-47.
(2.) Jones RG, Faust AM, Matthews RA. Quality team approaches in evaluating three automated haematology analyzers with five part differential capacity. Am J Clin Pathol 1995; 103:159-66.
(3.) Bentley SA, Johnson A, Bishop CA. A parallel evolution of four automated haematology analyzers. Am J Clin Pathol 1993; 100:626-32.
(4.) Drewinko B. Eight-parameter automated hematology analyzer. Comparison of two flow cytometric systems. Am J Clin Pathol 1982; 78:738.
(5.) England JM. Automated blood cell counters. In Hoffbrand AV, Brener MK(ed). Recent Advances in Hematology, Number eight. New York: Churchill Livingstone; 1996; 159-89.
(6.) Bain BJ, Neill PJ, Scott D, Scott TJ, Innis MD. Automated differential leucocyte counters: an evaluation of the Hemalog D and a comparison with the Hematrak. I. Principles of operation; reproducibility and accuracy on normal blood samples. Pathology. 1980 Jan; 12(1):101-9.
(7.) Bain BJ, Neill PJ, Scott D, Scott TJ, Innis MD. Automated differential leucocyte counters: an evaluation of the Hemalog D and a comparison with the Hematrak. II. Evaluation of performance on routine blood samples from hospital patients. Pathology. 1980 Jan; 12 (1):83-100.
(8.) Devreese K, De Logi E, Francart C, Heyndrickx B, Philippe J, Leroux-Roels G. Evaluation of the automated hematology analyzer Sysmex NE-8000. Eur J Clin Chem Clin Biochem. 1991 May; 29 (5):339-45.
(9.) Sheridan BL, Lollo M, Howe S, Bergeron N. Evaluation of the Roche Cobas Argos 5 Diff Automated Hematology analyzer with comparison to a Coulter STKS. Clin Lab Hem (2)1994; Jun: 117-30.
(10.) Renu S, Gayatri G, SK Sood. Quality Assessment of Automated Electronic Cell Counters. In Renu S(ed). Recent Advances in Hematology-2, 2nd edition. New Delhi: Arya; 2000; 286-96.
1. Deepshikha Dave
2. Amrish N. Pandya
PARTICULARS OF CONTRIBUTORS:
1. Tutor, Department of Pathology, Government Medical College Surat, Gurjarat.
2. Professor and Head, Department of Pathology, IHBT, Government Medical College, Surat, Gujarat.
NAME ADDRESS EMAIL ID OF THE CORRESPONDING AUTHOR:
Dr. Deepshikha Dave, F2, Payal Flats, Opposite Bhulka Bhavan School, Adajan, Surat--395009, Gujarat. E-mail: firstname.lastname@example.org
Date of Submission: 16/02/2014.
Date of Peer Review: 17/02/2014.
Date of Acceptance: 01/03/2014.
Date of Publishing: 20/03/2014.
Table 1: For platelet counts within subject factors Factor Mean Std. error 95% CL SYS * 251.2097 17.1589 216.8983 to 285.5211 PCE ([dagger]) 221.8710 12.3514 197.1729 to 246.5691 M_60 ([double dagger]) 265.3387 16.4263 232.4922 to 298.1852 ADVIA ([section]) 252.9194 16.9815 218.9628 to 286.8759 Table 2: For platelet counts Pairwise comparison Factor Vs Factor 2 Mean difference Std. error SYS PCE 29.339 6.907 M_60 -14.129 4.372 ADVIA -1.710 1.281 Factor Vs Factor 2 P (a) 95% CL (a) SYS PCE 0.0004 10.504 to 48.174 M_60 0.0119 -26.050 to -2.202 ADVIA 1.0000 -5.203 to 1.783 Table 3: For hemoglobin within subject factors Factor mean Std. error 95% CL SYS 12.0968 0.2852 11.5266 to 12.6670 PCE 11.6613 0.2839 11.0935 to 12.2291 M_60 11.3532 0.3056 10.7422 to 11.9642 ADVIA 11.9484 0.2845 11.3795 to 12.5172 Table 4: For hemoglobin Pairwise comparison Factor Vs Factor 2 Mean difference Std. error SYS PCE 0.435 0.0423 M_60 0.744 0.114 ADVIA 0.148 0.0323 Factor Vs Factor 2 P (a) 95% CL (a) SYS PCE <0.0001 0.320 to 0.551 M_60 <0.0001 0.432 to 1.055 ADVIA 0.0001 0.0604 to 0.236 Table 5: For WBC counts within subject factors Factor mean Std. error 95% CL SYS 7.1597 0.5618 6.0363 to 8.2831 PCE 7.2871 0.5791 6.1290 to 8.4451 M_60 7.4097 0.5363 6.3372 to 8.4822 ADVIA 7.2081 0.5688 6.0707 to 8.3454 Table 6: For WBC counts Pairwise comparison Factor Vs Factor 2 Mean difference Std. error SYS PCE -0.127 0.0479 M_60 -0.250 0.125 ADVIA -0.0484 0.0311 Factor Vs Factor 2 P (a) 95% CL (a) SYS PCE 0.0599 -0.258 to 0.00325 M_60 0.2975 -0.590 to 0.0903 ADVIA 0.7485 -0.133 to 0.364 Table 7: For RBC counts within subject factors Factor mean Std. error 95% CL SYS 4.2132 0.1028 4.0077 to 4.4188 PCE 4.1885 0.09343 4.0017 to 4.3754 M_60 4.1297 0.1053 4.9190 to 4.3403 ADVIA 4.2027 0.09998 4.0028 to 4.4027 Table 8: For RBC counts Pairwise comparison Factor Vs Factor 2 Mean difference Std. error SYS PCE 0.0247 0.0160 M_60 0.0835 0.0453 ADVIA 0.0105 0.00949 Factor Vs Factor 2 P (a) 95% CL (a) SYS PCE 0.7754 -0.0191 to 0.0684 M_60 0.4200 -0.0400 to 0.207 ADVIA 1.0000 -0.0154 to 0.0364 Table 9: For hematocrit within subject factors Factor mean Std. error 95% CL SYS 36.7097 0.7795 35.1510 to 38.2684 PCE 36.3258 0.7610 34.8042 to 37.8474 M_60 34.7677 0.8438 33.0805 to 36.4550 ADVIA 36.6613 0.7687 35.1241 to 38.1985 Table 10: For hematocrit Pairwise comparison Factor Vs Factor 2 Mean difference Std.error SYS PCE 0.384 0.242 M_60 1.942 0.312 ADVIA 0.0484 0.0593 Factor Vs Factor 2 P (a) 95% CL (a) SYS PCE 0.7047 -0.275 to 1.043 M_60 <0.0001 1.091 to 2.793 ADVIA 1.0000 -0.113 to 0.210 Table 11: For lymphocyte percentage within subject factors Factor mean Std. error 95% CL SYS 31.9500 1.7130 28.5247 to 35.3753 PCE 34.9710 1.9745 31.0227 to 38.9192 M_60 36.4839 1.7616 32.9613 to 40.0064 ADVIA 32.7194 1.7165 29.2871 to 36.1516 Table 12: For lymphocyte percentage Pairwise comparison Factor Vs Factor 2 Mean difference Std. error SYS PCE -3.021 1.351 M_60 -4.534 0.720 ADVIA -0.769 0.149 Factor Vs Factor 2 P (a) 95% CL (a) SYS PCE 0.1740 -6.705 to 0.663 M_60 <0.0001 -6.498 to -2.569 ADVIA <0.0001 -1.175 to -0.364 Table 13: For MCV within subject factors Factor mean Std. error 95% CL SYS 88.2290 1.6376 84.9545 to 91.5035 PCE 87.4371 1.6773 84.0832 to 90.7910 M_60 85.4677 1.6433 82.1817 to 88.7538 ADVIA 87.7742 1.6359 84.5031 to 91.0453 Table 14: For MCV Pairwise comparison Factor Vs Factor 2 Mean difference Std.error SYS PCE 0.792 0.397 M_60 2.761 0.595 ADVIA 0.455 0.0367 Factor Vs Factor 2 P (a) 95% CL (a) SYS PCE 0.3028 -0.290 to 1.874 M_60 0.0001 1.140 to 4.383 ADVIA <0.0001 0.355 to 0.555
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|Title Annotation:||ORIGINAL ARTICLE|
|Author:||Dave, Deepshikha; Pandya, Amrish N.|
|Publication:||Journal of Evolution of Medical and Dental Sciences|
|Date:||Mar 24, 2014|
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