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Comparative histological and histochemical studies of the larval tapeworms, Cyclustera ibisae and Glossocercus caribaenis. (Withdrawn).

Mummichogs (Fundulus heteroclitus) and striped killifish (Fundulus majalis) were collected from the North Inlet-Winyah Bay near Georgetown, South Carolina and examined for the presence of larval stages (metascestodes) of two dilepidid tapeworms (Glossocercus caribaenis and Cyclustera ibisae). These two species of tapeworms were found encysted in the liver and mesenteries of both fish hosts. The metacestodes were isolated and fixed in buffered formalin and processed for histological studies using standard (hematoxylin and eosin) staining procedures. Formalin-fixed tissues were also stained with Periodic Acid Schiff(PAS) and Gomori's Trichrome for histochemical caparisons of the two species of metacestodes. The cyst wall of G. carbiaensis was slightly thinner (~0.08 mm) than that of Cyclustera ibisae (~0.11 mm). In both species, however, the cyst wall appeared to be made of two distinct tissue layers. These metacestodes also differ in the thickness of integument (~0.01 mm for G. caribaensis and ~0.04 mm for C. ibisae). Other than the difference in general body form and size, these two metacestodes appear to be very similar. Histochemical analysis suggests presence of collagen in the tegument and out cyst wall, muscle fibers below the tegument and around the scolex area, and keratin at the base of the hooks in both species. Interestingly, very little or no glycogen was detected in any of the tissues examined. Additional histochemical tests could provide clues to distinguish between the two species of metacestodes and possibly, to understand their metabolism.
Avery Tomlinson, Edna Steele
Department of Biology
Converse College
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Author:Tomlinson, Avery; Steele, Edna
Publication:Bulletin of the South Carolina Academy of Science
Article Type:Brief Article
Geographic Code:1U5SC
Date:Jan 1, 2003
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