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Colonic epithelial nodular hyperplasia associated with strongyloidiasis in cats in the Amazon region, Para State, Brazil/Hiperplasia epitelial nodular no colon de felinos associada a estrongiloidiase na regiao Amazonica, Para, Brasil.

Strongyloides spp. are helminths that belong to the Rhabditoidea superfamily and Strongyloididae family and can have both free-living or parasitic reproductive cycles. The parasitic phase is completed by adult parthenogenetic females in the digestive tract of several animal species, including humans. These adult females have a thin, thread-like shape and are a few millimeters long (URQUHART et al., 1991; KASSAI, 1999).

Strongyloidiasis in domestic cats has been associated with S. felis, S. planiceps, S. stercoralis and S. tumefaciens. In the three former species, adult female parasitizes the small intestines; however, infection by S. tumefaciens is associated with the formation of nodules on the mucosa of the large intestine (PRICE & DIKMANS, 1941; MALONE et al., 1977; LEVINE et al., 1979; BROWMAN et al., 2002). In Brazil, studies concerning strongyloidiasis in cats are scarce; there are reports of parasitism by S. stercoralis in domestic cats (VICENTE et al., 1997) and by an unidentified species of Strongyloides in a wild feline (Leopardus tigrinus) (SANTOS et al., 2009).

The present study reports two cases of intestinal strongyloidiasis in cats. Animals were male adult mongrel cats. In the first case, in November 2012, a debilitated stray cat exhibiting muscle weakness and icteric mucous membranes was rescued. The cat died on the way to the clinical care and was submitted to necropsy. The main gross lesions included moderately icteric carcass, enhanced lobular pattern of the liver, splenomegaly, lymphadenomegaly and several 0.2 to 2.0cm diameter whitish nodules protruding from the colon mucosa and exhibiting a punctate opening facing the intestinal lumen. In the second case, in April 2013, a cat died after chronic diarrhea and progressive weight loss. The only gross lesions reported by the attending veterinarian were whitish nodules on the mucosa of the large intestine, similar to those described in the first case.

Fragments of several organs were collected, fixed in 10% formalin, processed routinely for histology, sectioned at 5^m, stained with hematoxylin and eosin, and observed under optical microscopy.

Histopathology revealed that the large intestine nodules were composed of projections of the mucosal epithelium into the submucosa, which formed tubules lined by a single layer of columnar epithelium with high cellularity (1-2 mitosis / obj. 40) and rare goblet cells (Figure 1A). Lumen of the tubules exhibited transversal sections of nematodes with paired genital tract, uninucleate eggs, and larvae, which were compatible with parasites belonging to the Strongyloididae family. Larvae were also observed in the interstitial tissue between the tubules and the limit of the nodules. Strongyloides spp. were characterized by the presence of rhabditiform eggs, larvae, and adults on cross sections. Eggs measuring 5 to 16 [micro]m in diameter were delimited by a thin cuticle. Adult females ranged from 39 to 63 [micro]m in diameter. Sometimes it was possible to distinguish structures, such as uterus, ovary, intestine, an external muscle layer and, rarely, the lateral chords, in adults (Figure 1A, B and C). In some cross sections of females, it was possible to recognize eggs in the uterus (Figure 1D) (GARDINER & POYNTON, 1999). There was a small number of lymphocytes, plasma cells, and macrophages bordering some nodules and interspersed between the tubules. Fragments of the large intestine, which were in formalin and had not been processed for histology, were dissected in an attempt to recover adult parasites; however, only larvae were recovered. These larvae exhibited smooth tegument, rounded head with a short buccal cavity, and a filariform esophagus occupying approximately one third of the body length. The intestinal lumen was straight, and it had a thin tail.

The first cat also exhibited significant lesions in the liver, which were characterized as moderate, focally extensive, necrosuppurative hepatitis with intralesional bacterial colonies. No significant lesions were observed in the few tissues submitted for histology from the second cat.

Histological alterations reported in the large intestine nodules from the cats in this study were identical to those described in parasitism by S. tumefaciens (PRICE & DIKMANS, 1941; MALONE et al., 1977; BOWMAN et al., 2002). Specific parasite identification was not possible in the cases presented here because only formalinfixed samples were available for analysis and only larvae were obtained after dissection of tissues. However, the pattern of tissue response to the parasite observed in the cats of the present study and in the literature on S. tumefaciens has not been described in parasitism by other Strongyloides species in domestic cats.

Lesions in the cats of the present study were considered as hyperplasia due the high cellularity and well-differentiated epithelium present in nodules, continuity with the adjacent colonic mucosal crypts, and rarefaction of goblet cells. Few lymphocytes, plasma cells, and macrophages observed, occasionally bordering and interspersed with the hyperplastic nodules, could either be remnants of lymphoid clusters or a reaction to the parasite. Thus, similarly to those described by other authors (MALONE et al., 1977; BARKER et al., 1993; BOWMAN et al., 2002), lesions observed in the cats of this study were interpreted as hyperplasia of the colonic crypt epithelium into the lymphoid clusters. However, some authors characterize this type of lesion as an adenomatous tumor (PRICE & DIKMANS, 1941).

Microscopic findings are characteristic; however, lymphoid cluster hyperplasia or intestinal lymphoma should be considered when evaluating the gross findings (LINGARD et al., 2009). Other differential diagnoses to be considered are colorectal polyps and intestinal carcinomas (CRIBB, 1988).

Antemortem diagnosis of strongyloidiasis in cats, particularly caused by S. tumefaciens, is complex because this parasitism is rarely associated with diarrhea (BARKER et al., 1993). It is unlikely that the lesions observed in the cases were sufficient to cause diarrhea because they were restricted to lymphoid clusters. Diarrhea was reported in only one of the cats. However, it was not possible to establish whether parasitism was the main cause of the diarrhea. In the other cat, death was attributed to liver failure, and parasitism was an incidental finding. It was not possible to determine the predisposing factors to parasitism in the cats of this study. Both animals had free access to the streets and had not received proper deworming medication. This access to various environments increased the possibility of contact with infective larvae and the development of parasites. Some limiting factors for the diagnosis of strongyloidiasis are related to the fecal parasitological methods routinely employed. The common stool examination methods require multiple stool samples to achieve higher sensitivity; therefore, the gold standard for the diagnosis of Strongyloides infection is serial stool examination, which is not commonly performed. The Baermann technique after fecal culture is another useful method, but it also not commonly performed in feline medicine (SPEARE & TINSLEY, 1987). The data presented here indicate that the possibility of infection by this Strongyloides species in cats in Brazil should be investigated.

http://dx.doi.org/10.1590/0103-8478cr20160328

Received 03.30.16

Approved 08.15.16

Returned by the author 10.24.16

CR-2016.0328.R1

ACKNOWLEDGEMENTS

To the Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior, Programa Nacional de Pos-doutorado (CAPES-PNDN) for the postdoctoral scholarship granted to the co-author Oliveira C. A.

REFERENCES

BARKER, IK. et al. The alimentary system. In: JUBB, K.V.F. et al. Pathology of domestic animals. San Diego, Academic Press, 1993. V.2, cap.1, p.1-318.

BOWMAN, D.D. et al. Feline clinical parasitology. Ames: Iowa State University, 2002. cap.4, p.239-241.

CRIBB, A.E. Feline gastrointestinal adenocarcinoma: A review and retrospective study. Canadian Veterinary Ciencia Rural, v.47, n.1, 2017. Journal, v,29, n.9, p.709-712, 1988. Available from: <http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1680855/>. Accessed: Feb. 15, 2016.

GARDINER, C.H.; POYNTON, S.L. An atlas of metazoan parasites in animal tissues. Washington, DC.: Armed Forces Institute of Pathology, 1999. 64p.

KASSAI, T. Veterinary helminthology. Oxford: ButterworthHeinemann, 1999. 260p.

LEVINE, N.D. Nematode parasites of domestic animals and of man. 2.ed. Minneapolis, Minn.: Burgess Pub. Co., 1979. p.477.

LINGARD, A.E. et al. Low-grade alimentary lynphoma: clinicopathological findings and response to treatment in 17 cases. Journal of Feline Medicine and Surgery, v. 11, p.692-700, 2009. Available from: <http://www.ncbi.nlm.nih.gov/ pubmed/19576832>. Accessed: Feb. 15, 2016. doi: 10.1016/j. jfms.2009.05.021.

MALONE, J.B. et al. Strongyloides tumefaciens in cats. Journal of American Veterinary Medical Association, v. 171, n.3, p.278-280, 1977. Available from: <http://www.ncbi.nlm.nih.gov/ pubmed/893214>. Accessed: Feb. 15, 2016.

PRICE, E.W.; DIKMANS, G. Adenomatous tumors in the large intestine of cats caused by Strongyloides tumefaciens, n. sp. Proceedings of the Helminthological Society of Washington, v.8, p. 41-44, 1941. Available from: <http://science.peru.edu/COPA/ ProcHelmSocWash_V8_N2_1941I.pdf>. Accessed: Feb. 15, 2016.

SANTOS, K.R.; et al. First report of Strongyloides sp. in Leopardus tigrinus in the municipality of Botucatu, State of Sao Paulo, Brazil. Revista Brasileira de Parasitologia Veterinaria, v.18, supl.1, p.77-79, 2009. Available from: <http://www.scielo.br/pdf/rbpv/v18s1/ a16v18s1.pdf>. Accessed: Feb. 15, 2016. doi:10.4322/rbpv.018e1016.

SPEARE, R.; TINSLEY, D.J. Survey of cats for Strongyloides felis. Autralian Veterinary Journal. v. 64, n.6, p. 191192, 1987. Available from: <https://www.researchgate.net/ publication/19528433_Survey_of_cats_for_Strongyloides_felis>. Accessed: Feb. 15, 2016.

URQUHART, G.M. et al. Parasitologia veterinaria. Rio de Janeiro: Guanabara Koogan, 1991. 57p.

VICENTE, J.J. et al. Nematoides do Brasil. Parte V: Nematoides de mamiferos. Revista Brasileira de Zoologia, v.14 Supl.1, p.452, 1997. Available from: <http://www.scielo.br/pdf/rbzool/v14s1/ v14s1.pdf>. Accessed: Feb. 15, 2016.

Marcio Alan Oliveira Moura (1) Ediene Moura Jorge (2) Kelly Karoline Gomes do Nascimento (1) Gabriela Riet-Correa (3) Isis Abel (3) Gustavo Goes Cavalcante (3) Carlos Alberto de Oliveira (3) Pedro Soares Bezerra Junior (3) *

(1) Faculdade de Medicina Veterinaria, Instituto de Medicina Veterinaria (IMV), Universidade Federal do Para (UFPA), Castanhal, PA, Brasil.

(2) Veterinario independente, Castanhal, PA, Brasil.

(3) Programa de Pos-graduacao em Saude Animal na Amazonia (PPGSAAM), Instituto de Medicina Veterinaria (IMV), Universidade Federal do Para (UFPA), Rodovia BR 316, Km 61, Bairro Saudade I, 68743-080, Castanhal, PA, Brasil. E mail: p.s.bezerra.junior@gmail.com. * Corresponding author.

Caption: Figure 1--A. Nodule in the large intestine composed by projections of the mucosal epithelium into the submucosa, forming tubules. HE, obj. 5. B. Lumen of the tubules exhibiting transverse sections of helminths, eggs, and larvae. HE, obj. 10. C. Detail of the larvae (Strongyloides spp.) inside the tubules (long arrow) and in the adjacent interstitial tissue (short arrow). HE, obj. 20. D. Detail of an adult female worm (Strongyloides spp.) and its internal organs: u: uterus; cl: lateral chords; i: intestine; m: musculature; o: ovary. HE, obj. 40.
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Title Annotation:texto en ingles
Author:Moura, Marcio Alan Oliveira; Jorge, Ediene Moura; do Nascimento, Kelly Karoline Gomes; Riet-Correa,
Publication:Ciencia Rural
Date:Jan 1, 2017
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