Clinicopathological study of diseases homing and infiltrating bone marrow in children less than 3 years of age.
Cancer is a metastatic disease. Ideally cancer cells can metastasise towards many organs of the body. Clinically it has been demonstrated that cancer cells prefer to migrate to certain distant organs of the body such as bone, lung, liver, brain or the adrenal gland. In children, neuroblastoma, rhabdomyosarcoma, Ewing's sarcoma and retinoblastoma account for the majority of metastases .
Bone marrow trephine biopsy is particularly valuable when aspirate cannot be obtained due to fibrosis or densely packed tumour cells. It is also important in the initial staging of certain paediatric tumours, such as neuroblastoma, rhabdomyosarcoma, primitive neuroectodermal tumour, and Ewing's tumour . It is therefore considered imperative to rule out marrow involvement in any malignancy where curative treatment is considered. Early and accurate diagnosis with prompt management significantly improves the patient outcomes.
In this present series we aim to study the clinical presentation, peripheral smear examination and bone marrow findings of diseases homing in and infiltrating the marrow in children less than 3 years of age.
MATERIAL AND METHODS: The present retrospective study was undertaken for a period of 5 years from June 2005 to May 2010 at Department of Pathology , Paediatric Referral Hospital. A Total of 194 Bone marrow aspirations and biopsies were done during this period in children aged less than 3 years. Children older than 3 years were excluded from the study. Detailed history, clinical signs and symptoms of the patient were obtained from the patients records. Complete haemogram and bone marrow studies were done.
RESULTS: A total of 194 Bone marrow aspirations and biopsies were done in children aged less than 3 years. Mean age at diagnosis was 23.7 [+ or -] 9.34 months (Range 10 - 36 months). In all these cases clinical examination, ultrasound study, peripheral smear examination and bone marrow study lead us to conclusive diagnosis. Bone marrow infiltration was found in 13 patients (6.7%). In majority of children, bone marrow was infiltrated by lymphoma (Figure 1A, 1B), followed by neuroblastoma (Figure 2A, 2B), Neimann Pick Disease (Figure 3), Gaucher's disease (Figure 4), rhabdomyosarcoma (Figure 5A, 5B) and Langerhans Cell Histiocytosis (Figure 6).
Out of the 13 cases with Bone marrow infiltration, 38.46% were cases of lymphoma, with male to female ratio being 1:4. In 23.07% of the children, bone marrow was infiltrated by neuroblastoma. While 7.69% of cases showed their bone marrow infiltrated by rhabdomyosarcoma and Langerhans Cell Histiocytosis. The rest of the 23.07% cases were storage disorders which included Neimann Pick disease and Gaucher's disease.
Incidence of bone marrow infiltration was observed to be higher in male children in all the cases except than in lymphoma. Bone marrow findings of children less than 3 years are tabulated in Table 1.Clinically the children with bone marrow infiltrations presented usually with constitutional symptoms. Other features of bone marrow involvement in the affected children included recurrent vomiting, haematemesis, melena, haematuria, failure to thrive, hepatosplenomegaly and bone pains (Table 2).
DISCUSSION: Bone marrow examination is one of the most common and relatively safe invasive procedures done routinely in paediatric units. It is commonly done for the assessment of unexplained cytopenias and malignant conditions like leukaemia. It is also done for the diagnosis or staging of a neoplasm and storage disorders. .
Chemokines play an important role in tumour cell homing to the bone marrow microenvironment Stromal cell derived factor-1 (SDF-1) is expressed by bone marrow stroma cells and helps in sequestering mature B cells to the bone marrow. CXCR4, a G protein coupled cell surface receptor is expressed by both normal and malignant haematopoietic cells, and also includes B and T cell non-Hodgkin lymphoma, multiple myeloma, acute and chronic leukaemias. Solid tumours like breast, ovarian, prostate, rhabdomyosarcoma, and neuroblastoma also express the CXCR4 receptor and metastasise to bone in an SDF-1 dependent manner .
The identification of metastatic cells in bone marrow do not pose much of a problem because the cells appear unfamiliar to the normal haematopoietic tissue, however sometimes their morphology may not reveal the exact site of origin. The morphology of metastatic tumour cells must be correlated with the clinical presentation and histopathological findings of the tumour to confirm the final diagnosis .
In one series, bone marrow aspirates of 79 children with malignant solid tumours showed infiltrations in 28 patients. Bone marrow was infiltrated by neuroblastoma in 21 cases, embryonal rhabdomyosarcoma in 3 cases, osteogenic sarcoma in 2 cases, Ewing's sarcoma in 1 case and retinoblastoma in 1 case .
According to another study, 213 children with various solid tumours were reviewed. 493 bone marrow aspirates from these patients were analysed. 60 patients showed bone marrow infiltrations. Bone marrow was infiltrated by neuroblastoma in 49 cases, rhabdomyosarcoma in 5 cases, retinoblastoma in 3 cases, ganglioneuroblastoma in 2 cases and miscellaneous sarcomas in 1 case .
In the present study 13 children had bone marrow infiltration. Bone marrow was infiltrated by lymphoma in 5 cases, neuroblastoma in 3 cases, rhabdomyosarcoma in 1 case, Langerhans cell histiocytosis in 1 case, Niemann Picks Disease in 2 cases and Gaucher's disease in 1 case.
One study reviewed 30 children with undifferentiated lymphoma, non-Burkitt's type, bone marrow involvement was present in 70% . While another series reported lymphomas as the commonest condition infiltrating the bone marrow .
Our study reported the incidence of neuroblastoma infiltrating the bone marrow to be around 23.07%. According to a study which included 48 cases of neuroblastoma, bone marrow involvement by neuroblastoma was docmented in 20 patients (42%) .
In the present study the incidence of storage disorders infiltrating the bone marrow was 23.07% which included 2 cases of Neimann Pick Disease and 1 case of Gaucher's disease. Our findings were consistent with that of similar study .
Comparison of the present study with available literature is depicted in Table 3. Lymphoma was commonest condition to infiltrate the bone marrow in our study as similarly reported by other series. Some authors reported neuroblastomas as the most common condition infiltrating the bone marrow [10, 11]. While our study showed neuroblastomas and storage disorders as second most common conditions to infiltrate the bone marrow.
CONCLUSION: Bone marrow examination in children should be done meticulously as the identification of metastatic cells in bone marrow is not that problematic. Closed trephine needle biopsy of the bone marrow has become a recognised procedure in the assessment of many malignant and benign diseases in adults; however, its role in paediatric pathology has not yet been defined. Bone marrow aspiration and biopsy gives a diagnosis for heterogeneous group of diseases involving haematopoietic cells and other diseases infiltrating bone marrow. Bone marrow examination is fastest, easiest, cheapest and simplest technique. The current study highlights the significance of bone marrow evaluation in the diagnosis of diseases homing and infiltrating the bone marrow in children.
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(1.) M. Ramani
(2.) D. Ranganath
(3.) K. Geetha
(4.) Kazi Wajid Husain
(5.) J. Khyati Kiran
(6.) Puja Deshmukh
PARTICULARS OF CONTRIBUTORS:
(1.) Professor, Department of Pathology, Niloufer Hospital, Hyderabad.
(2.) Professor, Department of Paediatrics, Niloufer Hospital, Hyderabad.
(3.) Assistant Professor, Department of Pathology, Niloufer Hospital, Hyderabad.
(4.) Post Graduate, Department of Pathology, Niloufer Hospital, Hyderabad.
(5.) Undergraduate, Third Year M.B.B.S., Osmania Medical College, Hyderabad.
(6.) Post Graduate, Department of Pathology, Niloufer Hospital, Hyderabad.
NAME ADDRESS EMAIL ID OF THE CORRESPONDING AUTHOR:
Dr. M. Ramani, Professor, Department of Pathology, Niloufer Hospital, Institute Of Child Health, Andhra Pradesh, Hyderabad, India. Email- firstname.lastname@example.org
Date of Submission: 07/07/2013.
Date of Peer Review: 08/07/2013.
Date of Acceptance: 14/08/2013.
Date of Publishing: 21/08/2013
Table 1: Bone marrow findings in children aged less than 3 years. NUMBER OF BONE MARROW FINDINGS PATIENTS MALE FEMALE NON HODGKINS LYMPHOMA 5 1 4 NEUROBLASTOMA 3 3 0 RHABDOMYOSARCOMA 1 2 0 LANGERHAN CELL HISTIOCYTOSIS 1 1 0 NEIMANN PICK DISEASE 2 1 0 GAUCHERS DISEASE 1 1 0 Table 2: Clinical features of cases with bone marrow infiltrations in our study. SYMPTOMS AND SIGNS Constitutional symptoms Recurrent vomiting Haematemesis Melena Haematuria Failure to thrive Hepatosplenomegaly Bone pains Table 3: Comparison of our study with available literature. BONE MARROW BASIL  FINKLESTEIN  SULLIVAN  FINDINGS et al. et al. et al. Neuroblastoma 75% 82% -- Rhabdomyosarcoma 11% 8% -- Lymphoma 0% 0% 70% Storage Disorders 0% 0% -- Others 14% 10% -- BONE MARROW MILLS  SYED  PRESENT FINDINGS et al. et al. STUDY Neuroblastoma 42% -- 23.07% Rhabdomyosarcoma -- -- 7.69% Lymphoma -- -- 38.46% Storage Disorders -- 23.07% 23.07% Others -- -- 7.69%
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|Title Annotation:||ORIGINAL ARTICLE|
|Author:||Ramani, M.; Ranganath, D.; Geetha, K.; Husain, Kazi Wajid; Kiran, Khyati J.; Deshmukh, Puja|
|Publication:||Journal of Evolution of Medical and Dental Sciences|
|Article Type:||Clinical report|
|Date:||Aug 26, 2013|
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