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Clinico-colonoscopic and histomorphological spectrum of colonic diseases in an academic tertiary care centre.

INTRODUCTION: The development of flexible endoscopes has led to a great increase in the examination and mucosal biopsy evaluation of all portions of the large intestine and sometimes terminal ileum. (1) Colonoscopic examination is important in the diagnosis and treatment of various suspected colonic diseases. It is a diagnostic procedure of choice for patients with diarrhea lasting for several weeks to months or for any bloody diarrhoea. (2) The procedure is used to identify early signs of colorectal cancer.

The colon and rectum can be sites for infections, inflammatory bowel diseases (IBD), vascular disorders, motor and mechanical conditions and various neoplasms. Macroscopically normal colonic mucosa may look pathologically inflamed.Thus without biopsy, significant inflammatory bowel disease may go unrecognized or be mistaken for a functional disorder. (2)

The American Society for Gastrointestinal Endoscopy has developed guidelines for colonoscopy based on the indications as 'generally indicated' and 'generally not indicated'. (3) The colonoscopic findings can subsequently be divided into clinically significant and clinically not significant based on the further therapeutic decisions and prognosis.

Biopsies are sought for specific diagnosis for determining the extent of disease and its response to therapy and for detecting complication. Colonoscopic biopsy provides the first source of tissue for most cases of colorectal carcinoma and therefore might become an important source for histopathological analysis. Colonoscopic mucosal biopsies have been shown to be most accurate indicator of the extent of involvement of the colon in inflammatory bowel disease.

The aims of this study were to correlate colonoscopic findings with the histopathological diagnosis obtained and also to study histopathological features of colonoscopic biopsy specimens.

MATERIALS AND METHODS: The study was a retrospective analysis of data from the Departments of Surgical Gastroenterology and Pathology between April 2012 and June 2013. Patient demographics, clinical details, colonoscopic findings and apparent pathology noted and the final histopathological diagnosis made were all recorded. Biopsies (n =107) which showed significant pathological findings were included in the study.

507 colonoscopies were performed at the department of Surgical Gastroenterology during this period. Biopsies were taken in cases with clinically relevant findings, which affected the therapeutic decisions and prognosis like colorectal cancers (CRC), polyps, inflammatory bowel diseases, strictures and proctitis or colitis secondary to radiation. Biopsies were also taken in some normal colonoscopies where irritable bowel syndrome (IBS) was suspected. The more severe diagnosis was used for analysis when there was more than one colonoscopic finding. These were sent to the department of pathology for histopathological examination.

All the colonoscopic biopsy specimens were immediately fixed in 10% formalin for 24 hours. It was then routinely processed and embedded with the mucosal surface uppermost. Five microns thick serial sections were prepared and stained with Hematoxylin and Eosin. Detailed study was performed under the light microscope. Adequacy of the biopsy was assessed and an attempt was made to correlate the histopathological diagnosis with colonoscopic diagnosis obtained. Special stains like Periodic acid-Schiff stain (PAS) and Van Gieson stains were used wherever necessary.

RESULTS: Biopsies taken from 107 cases showed significant findings on histopathology and these were included in the study. The clinically significant findings seen on colonoscopy included colorectal cancers, IBD, colonic tuberculosis, strictures, solitary rectal ulcers and polyps (Fig 1-8). Other relevant findings seen were GI bleed and extrinsic compressions from contiguous structures.

Ages ranged from 16-83 years, with a mean age of 47.48 [+ or -] 13.86 years. 68 biopsies were from men and 39 biopsies were from women. The male to female ratio was 1.74: 1. The presenting symptoms in the study population were chronic diarrhea, bleeding per rectum, abdominal pain, chronic constipation and weight loss, seen in 66.36%, 64.49%, 27.10%, 13.08% and 10.28% respectively. The duration of these symptoms ranged from 1 month to 2 years.

Among 107 biopsies, 82 biopsies were non-neoplastic, 25 biopsies were neoplastic. One case was classified as biopsy specimen inadequate or unsatisfactory for evaluation on initial biopsy; a repeat biopsy was requested and later was reported as chronic colitis. Overall 17 malignant lesions were detected on histopathology. The distributions of all the lesions are summarized in Tables 1, 2 and 3. (Fig 9-16)

DISCUSSION: Colonic conditions like infections, inflammatory bowel diseases, polyps and colorectal cancers are important lesions which often require colonic biopsy for their conclusive diagnosis. (4) Lower GI endoscopic evaluation is established as the diagnostic procedure of choice in the setting of diarrhea and lower GI bleed.

Out of 107 biopsies, 76.64% biopsies were non neoplastic, 23.36% biopsies were neoplastic lesions. These finding are similar with the other studies where non neoplastic lesions were detected more than the neoplastic lesions (66.3% vs. 28.9%, 61.3% vs. 38.7%). (4, 5) In another study the neoplastic lesions were seen more than the non-neoplastic lesions (56.2% vs. 43.1%). (6) Out of 82 biopsies diagnosed as non-neoplastic lesions, 45.12% biopsies comprised of chronic colitis, 9.76% biopsies were acute colitis, 24.39% were IBD and others made up 20.73%. Similar findings were seen in other previous study series where colitis was seen in 47.3% and 38.3% biopsies. (4, 7) A lower rate of colitis at 14.9% was seen in one previous study. (8)

Colitis was diagnosed in 54.88% of the biopsies, 9.76% acute colitis, and 45.12% chronic colitis. Acute colitis was characterized by neutrophilic infiltrate in the lamina propria. No crypt abscess or decrease in goblet cells was observed. Chronic colitis was characterized by well- preserved architecture of mucosal glands, normal goblet cells, and predominant lymphoblastic infiltrate in the lamina propria. Some biopsies also showed lymphoid follicles in the lamina propria. 7.32% showed tuberculosis and were characterized by presence of confluent granulomas with areas of caseation necrosis, aggregation of epithelioid cells, Langhans giant cells and lymphocytic infiltrate. 1.22% of biopsies each were diagnosed as amoebic colitis and malabsorption syndrome. Amoebic colitis was characterized by ulcerated mucosa and the exudates contained inflammatory cells along with amebic trophozoites some of which showed erythrophagocytosis. Malabsorption syndrome is characterized by villous atrophy of terminal ileum.

Six resected specimens of polyps were analyzed. Of these, 50% were inflammatory polyps, 33.33% were diagnosed as hyperplastic polyps and 16.67% lymphoid polyp. Inflammatory polyps were characterized by finger-like projections of submucosa covered by mucosa on all sides suggesting healing. The hyperplastic polyps were characterized by elongation of crypts in association with tall columnar cells giving the epithelium a crowded, tufted or crenated appearance. Goblet cells were relatively sparse. Lymphoid polyps were characterized by aggregates of mucosa associated lymphoid tissue with germinal centers.

18.69% of all biopsies were diagnosed as inflammatory bowel diseases. Previous studies also show a similar detection rate of IBD with 28.2% and 39% while one other study showed lesser incidence of IBD (14.9%). (4, 7, 8) Ulcerative colitis (UC) was predominantly seen in 90% of biopsies while Crohn's disease (CD) was significantly less seen at 10%. Three phases were described in UC, active phase;resolving phase and remission phase. (9) Majority of the cases of UC in our study were in active phase (88.89%) while remaining 11.11% were in resolving or remission phases. Active phase of UC showed epithelial necrosis, distortion of glandular pattern, and increase in the number of neutrophils, lymphocytes and plasma cells in the lamina propria, crypt abscesses, and decrease in the number of goblet cells with the bases of the crypts showing epithelial hyperplasia. Resolving phase was characterized by distorted and branched crypts with a villous surface, regenerative hyperplasia of the base of crypts, restoration of goblet cell population, reduction in the inflammatory cell infiltrate with few polymorphs and/or crypt abscess. In the remission phase, epithelial surface was flat, crypt atrophied and distorted, goblet cell population was normal. The lamina propria showed mild lymphoplasmacytic infiltrate. CD was characterized by the presence of small, multiple granulomas, foreign body type giant cells and lymphocytic infiltrate in the mucosa and submucosa.

Tuberculosis was seen in 7.32% of the non-neoplastic diseases, characterized by confluent granulomas, aggregation of epithelioid cells, langhans giant cells and caseation necrosis in the mucosa. Other previous studies have shown a higher percentage at 75% and 41%. (10, 11)

23.36% biopsies were classified as neoplastic lesions, of which 32% were benign, and 68% biopsies were malignant. Equal number of adenomas-serrated, villous, tubular and tubulovillous adenomas were seen. Serrated adenomas have dilatation of the crypt most pronounced at the base, presence of horizontally oriented crypts, large areas without endocrine cells with nuclear atypia. Tubular adenomas were characterized by closely packed epithelial tubules with little papillary infoldings. Both goblet cells and tall columnar cells were seen. Villous adenomas are characterized by finger like or leaf like villous process of lamina propria covered by dysplastic epithelium. Tubulovillous adenomas were characterized by features of both tubular and villous adenoma. We have seen a higher rate of dysplasia at 87.5% in our study compared to 20.6% in a previous study. (12)

Malignancies were seen in 15.89%. 94.11% were adenocarcinomas and 5.89% were melanoma. Out of these adenocarcinomas, 35.30% were well differentiated, 41.18% were moderately differentiated, 11.76% were poorly differentiated and 5.88% was mucin secreting type. Well differentiated adenocarcinomas are characterized by malignant glands infiltrating submucosa. The tumor cells are arranged in glandular pattern with some showing papillary process and were lined by tall columnar cells with hyperchromatic nuclei and showed mild nuclear stratification. Moderately differentiated adenocarcinomas show malignant glands with irregular outline. There is loss of nuclear polarity and variation in nuclear size and shape. Poorly differentiated adenocarcinomas display highly irregular and ill formed tubular structures. The tumor cells are seen in groups and cords and showed pleomorphism, hyperchromatic nuclei with prominent nucleoli and scanty cytoplasm. Mucin secreting adenocarcinomas with the tumor cells showing abundant clear cytoplasm with eccentric hyperchromatic nuclei. This case was PAS positive indicating presence of intracellular and extracellular mucin. These findings were, in other previous studies at 47%, 16.5%, and 52.0% vs. 45.5%, 70.1%, 14.9% vs. 7.5%, 13.4%, 14.9%, respectively. (9, 13-15) We can see a similar finding as in our study in the moderately and poorly differentiated adenocarcinomas with these studies.

CONCLUSION: Chronic diarrhea and bleeding per rectum were the most common presenting complaints. IBD and malignancy were detected in a significant number of the biopsies. Histopathological diagnosis correlated well with the colonoscopy diagnosis offered. Colonoscopic screening can detect early colonic neoplasms in asymptomatic adults. Greater awareness of the disease and understanding of pathogenesis on the part of the pathologist is necessary for a better improved diagnosis since the specimens are smaller in size.

REFERENCES:

(1.) Levin B, Lieberman DA, Mc Farland B, Andrews KS, Brooks D, Bond J, Dash C, Giardello FM, Glick S, Johnson D, Johnson CD, Levin TR, Pickhardt PJ, Rex DK, Smith RA, Thorson A, Winawer SJ; American Cancer Society Colorectal Cancer Advisory Group; US Multi-Society Task Force; American College of Radiology Colon Cancer Committee. Screening and surveillance for the early detection of colorectal cancer and adenomatous polyps, 2008: a joint guideline from the American Cancer Society, the US Multi-Society Task Force on Colorectal Cancer, and the American College of Radiology. Gastroenterology. 2008 May; 134(5): 1570-95.

(2.) Carpenter HA, Talley NJ. The importance of clinicopathological correlation in diagnosis of inflammatory conditions of colon: histological patterns with clinical implications. Am J Gastroenterol. 2000 Apr; 95(4): 878-896.

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(7.) Dickinson RJ, Gilmour HM, McClelland DB. Rectal biopsy in patients presenting to an infectious unit with diarrhoeal disease. Gut. 1979 Feb.; 20(2): 141-8.

(8.) Flick AL, Voegtlin KF, Rubin CE. Clinical experience with suction biopsy of rectal mucosa. Gastroenterology. 1962 Jun; 42: 691-705.

(9.) Morson BC. The large intestine. In: W St C Symmers, Ed. Systemic pathology. Edinburgh, Churchill Livingstone; 1987. p.313-416.

(10.) Tandon HD, Prakash A. Pathology of intestinal tuberculosis and its distinction from crohn's disease. Gut. 1972 Apr; 13(4): 260-9.

(11.) Bhargava DK, Kushwaha AK, Dasarathy S, Shrinivas, Chopra P. Endoscopic diagnosis of segmental colonic tuberculosis. Gastrointest Endosc. 1992 Sep-Oct; 38(5): 571-4.

(12.) Konishi F, Morson BC. Pathology of colorectal adenomas: a colonoscopic survey. J Clin Pathol. 1982 Aug; 35(8): 830-841.

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(14.) Jonasson L, Hallgrimsson J, Theodors A, Jonsson T, Magnusson J, Jonasson JG. Carcinoma of the colon in Iceland 1955-1989. A study on pathology.Laeknabladid. 2001 Feb; 87(2): 111-117

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Rangaswamy R [1], Sahadev R [2], Suguna B.V [3], Preethan K.N [4], Ranjeeta S.B [5]

AUTHORS:

[1.] Rangaswamy R.

[2.] Sahadev R.

[3.] Suguna B.V.

[4.] Preethan K.N.

[5.] Ranjeeta S.B.

PARTICULARS OF CONTRIBUTORS:

[1.] Professor, Department of Pathology, Kempegowda Institute of Medical Sciences, Bangalore.

[2.] Associate Professor, Department of Surgical Gastroenterology, Kempegowda Institute of Medical Sciences, Bangalore.

[3.] Professor, Department of Pathology, Kempegowda Institute of Medical Sciences, Bangalore.

[4.] Assistant Professor, Department of Surgical Gastroenterology, Kempegowda Institute of Medical Sciences, Bangalore.

[5.] Post Graduate Student, Department of Pathology, Kempegowda Institute of Medical Sciences, Bangalore.

NAME ADDRESS EMAIL ID OF THE CORRESPONDING AUTHOR:

Dr. Rangaswamy R., Professor, Department of Pathology, Kempegowda Institute of Medical Sciences, K.R. Road, V.V. Puram, Bangalore--560004.

Email--drrangs64@yahoo.co.in

Date of Submission: 07/12/2013.

Date of Peer Review: 09/12/2013.

Date of Acceptance: 23/12/2013.

Date of Publishing: 01/01/2014

Table 1: Classification of all Lesions

Diagnosis        Number   Total     %

Non neoplastic             82     76.63
Neoplastic                 25     23.37
  -- Benign        8              32.00
  -- Malignant     17             68.00
  Total                    107    100.00

Table 2: Distribution of Non Neoplastic
Lesions

Diagnosis                No. of     %
                         cases

Chroniccolitis             37     45.12
Ulcerativecolitis          18     21.95
Acute colitis              8       9.76
Tuberculosis               6       7.32
Inflammatory polyp         3       3.66
Solitary rectal ulcer      3       3.66
Crohn's colitis            2       2.44
Hyperplastic polyp         2       2.44
Amoebic colitis            1       1.22
Lymphoid polyp             1       1.22
Malabsorption syndrome     1       1.22
Total                      82     100.00

Table 3: Distribution of Neoplastic Lesions

                             Lesions             No    Total   %

Benign                                                 8       32.00

            No Dysplasia     Serrated             1            12.50
                             adenoma

            With Dysplasia                        7            87.50

            Serrated                          1
            adenoma

            Villous                           2
            adenoma

            Tubulovillous                     2
            adenoma

            Tubular                           2
            Adenoma

Malignant                                              17      68.00

            Adenocarcinoma                        16           94.12

                             Well             6
                             differentiated

                             Mod              7
                             differentiated

                             Poorly           2
                             differentiated

                             Mucin            1
            Melanoma         secreting            1            5.88
Total                                                  25      100.00
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Title Annotation:ORIGINAL ARTICLE
Author:Rangaswamy, R.; Sahadev R.; Suguna, B.V.; Preethan K.N.; Ranjeeta S.B.
Publication:Journal of Evolution of Medical and Dental Sciences
Article Type:Report
Geographic Code:9INDI
Date:Jan 6, 2014
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