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Clinical efficacy of Amoxycillin and Tazobactum combination for the management of bovine mastitis.


Mastitis, an inflammatory reaction of the mammary gland is usually caused by microbial infection and characterized by an influx of somatic cells, primarily polymorphonuclear cells into the mammary gland (Zhao and Lacasse, 2008). Bovine mastitis is recognized as one of the most economically important diseases of dairy cattle across the world owing to heavy financial losses incurred in the form of reduction in milk production, discarding of milk due to poor quality, decrease market value of animals due to quarter/s damage and cost rendered on Veterinary treatment. The present study was undertaken to evaluate the efficacy of Amoxycillin and Tazobactum combination in treatment of clinical mastitis in bovines.


A total of 23 buffaloes in 5-7 years age group were reported in months of July to December' 2010 with history of calving 12 days to 2 months back and acute swelling in one or two teats and associated quarters since 3-4 days. Milk from affected teats was watery to curdled depending upon severity of infection. All the presented cases were earlier treated locally with drugs like Intramammary infusions, Meloxicam and other antibiotics like Fortified Procaine penicillin and Ampicillin. Hot fomentation of affected quarter was also done by owners in most of the cases which further deteriorated the condition.

Clinical observation

The clinical examination of affected quarter revealed, swollen, hard udder, hot to touch. In most of the cases affected teats had become hard, red, painful with watery, clotted curdled to caseated milk came out from affected teats. The temperature, respiration rate, pulse rate and ruminal movement were normal in range.


The history, clinical finding and physical examination of udder were sufficient to make confirmatory diagnosis. To find out the involvement of other affected quarter or to assess the extent of damage, strip cup test, white side test, california mastitis test, mastitis detection strip test and electrical conductivity test were performed as per the standard procedures described by Singh et al. (2008). The milk samples from different quarter were collected aseptically and sent for antibiotic sensitivity test to select the most potent antibiotic as per the standard procedures described by Cruickshank et al. (1975). Antibiotic sensitivity test of milk from affected quarter revealed resistance to local bacterial population to Procaine Penicillin, Ampicillin, Chloramphenical, Erythromycin and sensitive to Amoxycillin, Gentamicin, Enrofloxacin and Tetracycline.


The affected animals were treated with Injection Intamox Za (Amoxycillin-3000mg + Tazobactum sodium-375 mg) @ 3375 mg IM for 5 days, Injection Melonexa (Meloxicam) @ 15 ml IM, Injection Anistamina (Chloraphenramine maleate) @ 10 ml IM, Injection Tribiveta (Vitamin [B.sub.1], [B.sub.6] and [B.sub.12]) @ 10 ml IM for 5 days. Out of these 23 animals, 16 (69.56%) animals showed recovery after 3 days of therapy and in remaining 7 animals this therapy was continued for further next 4 days. Out of these 7 animals, 4 (17.39%) animals showed complete clinical recovery after 7 days of therapy. The other 3 animals in which the teat / teats got partially sloughed were dried off by intramammary infusion of diluted solution of betadine (Intadine (a)). In the present study 86.95% of affected animals showed complete clinical recovery after 3-7 days of therapy of Intamox Z. So Intamox Z is highly effective on treatment of clinical mastitis in bovines.


Mastitis is considered as global problem of the dairy industry since long back (Radostitis et al., 2000). It has been recognized as most costly disease affecting dairy animals. The treatment of mastitis in early lactating phase is a weak spot and very often fails with conventional antibiotic therapy because of continuous drainage during milking, absence of immunocompitance of the affected udder and withdrawal of milk losses (Dash, 2000). Immediate prompt treatment has so far been considered the only way to avoid the much damage of udder tissue and prevent further complication.

Amoxycillin has remarkable activity against wide range of Gram positive and Gram negative aerobes and anaerobes. Tazobactum is semi synthetic beta-lactamase inhibitor. The combination of Amoxycillin and Tazobactum therefore provide a broad spectrum bactericidal activity along with inhibition of beta-lactamase.

In the present study out of 23 animals, 20 (86.95%) animals showed complete clinical recovery. Out of these 20 recovered animals 16 (80%) animal showed complete clinical recovery after 3 days of therapy and remaining 4 (20%) animal showed complete clinical recovery after 7 days of therapy. So, Amoxycillin and Tazobactum combination is highly effective (86.95%) against treatment of clinical mastitis in bovines.


Cruisckshank, R., Duguid, J.P., Marmion, B.P. and Swain, R.H.A. (1975). Medical Microbiology. Vol. 2: 12th edn. Churchill Living stone, New York,

Radostitis, O.M., Gay, G.C., Blood, D.C. and Hinchcliff, K.W. (2000). Veterinary Medicine. 9th Edn., Book Power formerly ELST. London.

Singh, K.P., Singh, S.V., Singh, J.P., Singh, B., Jaiswal, S. and Singh, H.N. (2008). Theraputic management of gangrenous mastitis in buffaloes. Intas Polivet 9: 255-57.

Zhao, X and Lacasse, P. (2008). Mammary tissue damage during bovine mastitis: causes and control. J. Anim. Sci. 86: 57-65.

K.P Singh * (1), S.V. Singh (2), J.P. Singh (2), Bhoopendra Singh (3), Praneeta Singh (4) and H.N. Singh (5)

Department of Veterinary Medicine

College of Veterinary Science and Animal Husbandry

Narendra Deva University of Agriculture and Technology


Faizabad--224229 (Uttar Pradesh)

(1.) Veterinary Officer and Corresponding Author; Government Veterinary Hospital, Anand Nagar Bhitari, Faizabad, Uttar Pradesh


(2.) Assistant Professor

(3.) Assistant Professor, Department of Animal Reproduction, Gynaecology and Obstetrics

(4.) Assistant Professor, Department of Livestock Product Technology, C.V.A.Sc., GBPUAT, Pantnagar

(5.) Professor and Head, Department of Surgery and Radiology

(a) - Brand of Intas Animal Health, Ahmedabad
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Title Annotation:Clinical Article
Author:Singh, K.P.; Singh, S.V.; Singh, J.P.; Singh, Bhoopendra; Singh, Praneeta; Singh, H.N.
Publication:Intas Polivet
Article Type:Report
Geographic Code:9INDI
Date:Jan 1, 2013
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Next Article:A comparative evaluation of two therapies for the management of acute bovine mastitis.

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