City of Hope obtains United States patent.
City of Hope (Duarte, CA) has patented genetically engineered,
CE7-specific redirected immune cells expressing a cell surface protein
having an extracellular domain comprising a receptor which is specific
for CE7, an intracellular signaling domain, and a transmembrane domain,
and methods of use for such cells for cellular immunotherapy of CE7+
neuroblastoma. In one embodiment, the immune cell is a T cell and the
cell surface protein is a single chain FvFc:.zeta. receptor where Fv
designates the V.sub.H and V.sub.L chains of a single chain monoclonal
antibody to CE7 linked by peptide, Fc represents a
hinge-C.sub.H2-C.sub.H3 region of a human IgG.sub.1, and .zeta.
represents the intracellular signaling domain of the zeta chain of human
CD3. DNA constructs encoding a chimeric T-cell receptor and a method of
making a redirected T cell expressing a chimeric T cell receptor by
electroporation using naked DNA encoding the receptor are also
disclosed. (US 7,354,762)