Citologias vaginales: una fuente clave de informacion del ciclo estral en murcielagos neotropicales.
Estrous cycles in Neotropical bats occur yearround (Racey, 2009; Altringham, 2011), and are generally associated with local flowering and fruiting events (Racey, 1982; Kunz et al., 2009). In tropical ecosystems, bat reproductive events involve different strategies depending on climatic variables such as rainfall and its effect on the availability of nutritional resources (Estrada and Coates-Estrada, 2001); whereas in temperate areas reproduction tends to be seasonal due to climatic restrictions (Willig, 1985; Barclay et al., 2004). Although environmental conditions, in general, are more stable in the tropics (Altringham, 2011), seasonally dependent changes in the abundance of fruits, flowers, and insects are usually contingent on levels of precipitation (Montiel et al., 2011), which trigger the reproductive activity of many tropical species (Wilson, 1979; Porter and Wilkinson, 2001; Sperr et al., 2011). The ecology of bat species and specifically reproduction-related traits have received considerable attention due to the diversity, heterogeneity, and functional importance of Neotropical bats across a diversity of tropical ecosystems. As such, accurate and low-cost methods are likely to prove of great value in understanding key aspects of the reproductive ecology of Neotropical bats and the impact of habitat fragmentation on their reproductive cycles.
Studies on bat reproductive cycles, including determination of reproductive peaks, inactive periods, and female reproductive stages are often based on assessments of an individual's reproductive stage and posterior association with environmental determinants (Fleming et al., 1972; Wilson, 1979; Costa et al., 2007; Duarte and Talamoni, 2010; Santos-Moreno et al., 2010; Montiel et al., 2011). For this, the reproductive characterization of each individual is usually based on a set of external reproductive signs (Fleming et al., 1972; Dinerstein, 1986; Mena and Williams de Castro, 2002; Tschapka, 2005; Costa et al., 2007; Duarte and Talamoni, 2010). However, the fidelity, quality, and objectivity of data collection using this approach largely depend on the experience of the researcher in assessing each species and therefore the chances of subjective or inaccurate assessment are considerable. To mitigate potential inaccuracies, some authors have proposed the use of cytological smears of vaginal wall cells as a more accurate and objective method for estimating the reproductive stage of adult bats (Laska, 1990; Elizalde-Arellano et al., 2008; Wang et al., 2008; Racey, 2009). This method is based on the identification of the estrous cycle as a sequence of stages (anestrus, proestrus, estrus, and metaestrus), by counting and characterizing the different types of vaginal cells present in the vaginal wall (Racey, 2009). Each stage during the cycle has a singular and differential cell composition involving different proportions of parabasal, intermediate, and superficial nucleated or anucleated cells. This allows to determine estrous cycle stages, estimated through vaginal wall cell type counts (Nelson, 2000; Felipe et al., 2001; Touma et al., 2001; Escobar Botero et al., 2004; Ji et al., 2008).
The objective of our study was to design and test a new protocol using vaginal smears technique for reproductive characterizations in Neotropical bats.
MATERIALS AND METHODS
Sampling was performed on small living female bats (Microchiroptera), in the field or under laboratory conditions, at all stages of the reproductive cycle. The protocol was divided into three stages from field collection to laboratory analysis:
Stage 1. Vaginal mucosa cell sampling
This method represents a low cost approach, in which samples are relatively easy to collect and analyze, and can provide useful information with relatively little sampling effort in the field. The step-by-step process is described below.
A drop of sterile water (pH 5) solution was taken using a micropipette Socorex [R] (0.1 - 2 [micro]l) with tips Brand [R] (0.1-20 [micro]l). The standard measure for adult females of large species with a weight of more than 40 g was 2 [micro]l (e.g. Phyllostomus spp., Artibeus spp., Noctilio spp.). In the case of juvenile females and small species with a weight of less than 40 g (e.g. Thyroptera spp., Myotis spp., Lasiurus spp., Dermanura spp., Glossophaga spp.) the micropipette must be set to a volume of 1.5 [micro]l.
The saline solution was introduced into the female's vagina by inserting a short length of the micropipette tip to avoid harming the individual.
Wait from five to ten seconds to obtain a suspension of the vaginal mucosa cells.
Aspirate the saline solution from the vagina in the same way using the micropipette tip and transfer the suspension to a microscope slide. To transfer the suspension of saline onto the microscope slide, an S motion was used while expelling the suspension on the center of the slide. The sample was allowed to dry at ambient temperature for five to ten minutes inside a slide box to avoid dust contamination
The sample was covered with a methanol (90%) spray in order to fix it, and the alcohol was allowed to evaporate completely.
The slide was labeled and storaged in a box.
Humidity during sample preparation in the field in humid tropical regions should be minimized using high-performance desiccants (e.g. Silica gel). Use of latex-gloves and a facemask when handling samples in the field and the laboratory help avoid sample contamination.
Stage 2. Sample staining
In this study we used Gram-stain technique because it's simplicity, low cost and allows the observation of the relationships between nucleus and cytoplasm (Public Health England, 2015).
Stage 3. Cell count
For the description of the different cellular types, the combination and proportions of cells and the stages of the estrous cycle, cell counting can be performed both under laboratory and/or field conditions. For this, cells are counted through phase contrast microscope with 40x magnification. Each microscope slide is divided into four sections and at least 50 cells are counted in each section. A minimum of 200 cells must be counted in each sample in order to accurately estimate the proportion for each cell type. The number of cells of each type (parabasal, intermediate, nucleated, and non-nucleated superficial) present in each sample is recorded (Nelson, 2000; Escobar Botero et al., 2004), and the percentage of each cell type is calculated per sample. Based on the observed cell-type proportions it is possible to determine female reproductive status.
Field test of the protocol
We tested the protocol under field conditions in order to estimate its accuracy, feasibility, and agreement with traditional external traits assessment. Vaginal smears were obtained from females caught in the Department of Cordoba, northeastern Colombia, in August 2011 to March 2012. The specific study areas were located in the municipalities of Buena Vista (N.08[degree]11'05.3"; W.75[degree]31'49.2"), Canalete (N.08'30'37.1"; W76[degree]06'12.9"), Monteria (N.08[degree]44'32.4"; W.76[degree]19'23.4"), and Los Cordoba (N.08053'20.0";W.76018'42.6"). These areas are characterized by agricultural and livestock land-use, low human population density with fragments of tropical dry forest. For the manipulation of the individuals we followed all the recommendations American Society of Mammalogist (Sikes et al., 2011).
The external reproductive status of each captured female was recorded (status of nipples, pregnancy, and lactancy) (Racey, 2009; Duarte and Talamoni, 2010; Santos-Moreno et al., 2010; Montiel et al., 2011). The laboratory steps, including Gram staining and cell type counting using a Nikon e400 microscope, were performed in the Laboratorio de Ecologia Funcional of the Pontificia Universidad Javeriana, Bogota, Colombia.
All analyses were performed using pooled results from all bat species sampled. Two sources of information on reproductive variables were evaluated: vaginal smears and external reproductive traits. The normality of the data set and comparative analyses (Kruskal-Wallis test) were performed using InfoStat software (Di Rienzo et al., 2015).
Traits of epithelial cells were characterized in the counting of vaginal smears, parabasal cell are circular or oval, the nucleus covers a large proportion of the cytoplasm (Fig. 1a), in the anestrous stage the parabasal cells were more prevalent than the other cell types (H: 107.68, p 0.0001). Intermediate cells have polyhedral forms and large area of cytoplasm, the presence of granular chromatin in these type of cells recorded in the small and granulated nucleus (Figure 1b), the proportion of these cells was the highest in the metestrous stage (H: 254.58, p 0.0001). Superficial nucleated cells have polyhedral forms, with a large cytoplasm and small nucleus, the nucleus is dark because of the presence of condensed chromatin showing the main difference with the Intermediate cells (Figure 1c). In the proestrous stage, superficial nucleated cells were significantly more abundant than other types of cells present in vaginal smears (H: 798.75 p 0.0001). Superficial non-nucleated cells have polyhedral forms and show absence of nucleus, cytoplasm is translucent and not plegated (Figure 1d), the estrous stage was characterized by the high prevalence of superficial anucleated cells in the samples (H: 588 p 0.0001).
[FIGURE 1 OMITTED]
A total of 457 individuals of seven species were characterized using external reproductive traits. The most abundant species in the total sample were Artibeus lituratus (27%), A. planirostris (25%) which together accounted 52% of all bats captured. The remaining 48% was represented by five species: Carollia castanea (14%), Phyllostomus discolor (12%), C. perspicillata (11%), Dermanuraphaeotis (8%) and Glossophaga soricina (3%). All species were frugivorous except G. soricina (nectarivore-insectivore) (Clarke et al., 2005). Females were characterized as inactive (309 individuals, 68%), followed by pregnant females (108 individuals, 23%) and lactating females (40 individuals, 9%). Examination of vaginal smears from these individuals indicated that the largest proportion of females were in proestrous (221 individuals, 48%), followed by the estrous (141 individuals, 31%), metestrous (70 individuals, 15%), and anestrous stage (25 individuals, 6%).
Of the 309 females characterized as inactive by external traits, 294 individuals (95%) were identified to be in an estrous stage following cytological examination. Only 15 females (5%) were characterized as inactive stage of reproductive activity both external and cytological analysis.
The diversity of reproductive strategies in bats reflects both the effects of the environmental conditions and the species-specific behaviors associated with the stages of the reproductive cycle (Racey, 2009). Despite the number of studies that have examined the reproductive ecology of bats, cytological examination of vaginal smears has been employed for just three species: C. perspicillata (Bonilla and Turriago, 1988; Laska, 1990), Diphylla ecaudata (Elizalde-Arellano et al., 2008), Rosettus leschenaultia (Zhang et al., 2007).
Data from vaginal smears and estrous cycle characterization can be used for evaluation of particular reproductive events during estrous cycle, including mating events, and sexual inactivity (Elizalde-Arellano et al., 2008). Cytological information has proved valuable for reproductive studies in many other mammalian species (Felipe et al., 2001; Touma et al., 2001; Elizalde-Arellano et al., 2008). Remarkably, incipient pregnancy, which is almost impossible to determine by external traits (Altringham, 2011; Montiel et al., 2011), was properly determined by our protocol, as part of the metestrous stage (Elizalde-Arellano et al., 2008), despite the uncertainty associated with our method. Special care is required during the classification of superficial nucleated and intermediate cells due to the morphological similarity of these two vaginal cells types. In the estrous and proestrous stages we observed an increase in the prevalence of superficial cells, both nucleated and non-nucleated morphotypes.
The cytological technique has the added advantage of avoiding female sacrifice while generating more accurate reproductive data than the external reproductive characterization. With complementary methods, cytological analyses could provide a combination of information on bat behavior and reproductive activity under field conditions. The possibility of more precise information on estrous cycle stage at individual-level will allow the analysis of both species-specific and population-specific reproductive strategies across different habitats, as well as improved understanding of social hierarchies and the study of social interactions in harem systems. In addition, information generated using cytological analyses may help evaluate bat population dynamics in relation to different anthropogenic disturbance scenarios and the potential effects of climatic change. We conclude that the availability of a standard protocol for the collection and analysis of vaginal smears should foster an improved understanding bat reproductive ecology, allowing to compare data in time and space and across species and contexts (spatial and seasonal).
We thank Drs. Trevor Williams (INECOL, Mexico), Jose F. Gonzalez-Maya (ProCAT Colombia, UNAM, Mexico), Jerrold Belant (Mississippi State University, USA), Carolina Valdespino (INECOL, Mexico), Maria Cecilia Londono (IAvH, Colombia) and Sergio Solari (UdeA, Colombia) for early comments on the manuscript. To the members of the Laboratorio de Ecologia Funcional (LEF-PUJ) for their help in the counting's of vaginal smears. The owners of all ranches kindly allowed access to field sampling sites. This study was funded by "Valorization of the goods and services of biodiversity for the sustainable development of Colombian rural landscapes" project (PUJ-ID PRY 003161). Collection permits were provided by Corporacion Autonoma Regional de los Valles del Sinu y San Jorge (CVS, permit number 1.3501). We are grateful for the valuable comments by the handling editor and the anonymous reviewers.
Recibido 21 diciembre 2015. Aceptado 13 abril 2016. Editor asociado: G Francescoli
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I. Mauricio Vela-Vargas (1,2), Laura Perez-Pabon (2), Paloma Larrain (2), and Jairo Perez-Torres (2)
(1) Proyecto de Conservacion de Aguas y Tierras--ProCAT Colombia. Carrera 13 No. 96-82, Oficina 205. Bogota, Colombia [Correspondence: I. Mauricio Vela-Vargas <firstname.lastname@example.org>]
(2) Laboratorio de Ecologia Funcional, Unidad de Ecologia y Sistematica (UNESIS), Departamento de Biologia, Pontificia Universidad Javeriana. Bogota, Colombia.
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|Author:||Vela-Vargas, I. Mauricio; Perez-Pabon, Laura; Larrain, Paloma; Perez-Torres, Jairo|
|Date:||Jun 1, 2016|
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