Cholera in Bidar district of Karnataka state, India.
In the present study the isolates were vibrio cholera eltor, ogawa and NAG vibrio. Phage type Basu Mukherjee [T.sub.2] and New Scheme [T.sub.27] were seen predominantly, apart from this [T.sub.24], [T.sub.21], [T.sub.13] were also seen in less numbers. And NAG vibrio Non-0139 was also isolated. This study was conducted first time from Bidar district, in Govt. Medical College & Hospital (Brims Bidar). So, study gives much importance to prevent the public diseases and guide for treatment
and management of the illness. Culture 57 (10.14%) were confirmed from Bidar, during the out-break a large number of acute gastroenteritis were admitted in Government Hospital (Brims) Bidar.
MATERIAL METHODS: A total of 562 patients suffering from acute gastroenteritis were admitted to Govt. Medical College and Hospital, Bidar, these patients included both children and adults, age ranging (2-60 years) and belonged to the low-socio economic group, and children are more in numbers.
These cases indicate only the tip of the ice berg, because many cases were admitted for acute watery diarrhea in the private hospital.
The most rapid presumptive method to identify the presence of vibrio, in the stool sample is to observe for darting type of motility in the hanging drop preparation of the stool; but culture is must as culture consider as gold standard method for identification. Because sometimes there will not be seen motility due to delay in submission of clinical sample to the laboratory or improper collection of clinical sample.
Faecal specimen collected from each patient was studied bacteriologically and was identified by a standard procedures. (3) Isolates of cholera strain put on VP (Voges--Proskaur), Methyl red, Indole, Cholera red, Oxidase, Susceptility to polymxin-B (50 units). Sero typing was performed using anti sera obtained from king Institute of preventive medicine, Guindy Chennai, Antimicrobial susceptility test was carried out by stokes disc diffusion method using the following antibiotics (High Media Laboratories Mumbai, India) Teracycline (30 [micro]g disc) chloramphenicol (30 [micro]g disc) Amoxycillin (100 [micro]g disc) ciprofloxacin (5 [micro]g disc) Furazolidone (5 [micro]g disc) co-trimoxazole (25 [micro]g disc) and Gentamicin (10 [micro]g disc). And randomly picked the isolate positive 25 strain of vibrio cholera were sent to National Institute of Cholera and enteric diseases (NICED) Kolkatta for cross check confirmation and sero type, bio type and phage typing.
RESULTS: Out of 562 faecal samples processed 57 (10.14%) were found positive for vibrio cholera, 39 (68.42%) isolates, belong to sero type ogawa and 18 (31.57%) isolates belong to NAG vibrio.
The isolates was also confirmed by the NICED Kolkata was out of 25 isolates 16 belong to eltor ogawa phage type [T.sub.2] in this 13 isolates belong to phage group [T.sub.27] and 6 isolates of each two belong to [T.sub.24], [T.sub.21], [T.sub.13] of new scheme. The remaining isolates of 6 belong to non-01/non-0139 phage type.
In our study cholera strains were isolated almost all the 12 months of the year. That means no seasonal variations, but epidemic are noticed in the month of May, June, July-2009 and Oct-2008.
DISCUSSION: In the present study 39 (68.42%) isolates were vibrio cholera 01 sero type ogawa, and 18 (31.57%) isolate were NAG vibrio non-ol/ non-0139. The phage typing was consistent with the overall country wide, epidemiological data M which reported Type [T.sub.27] to be predominant and with this our study revealed a high percentage of children affected in the outbreak. The main contaminating source of outbreak was traced to be the drinking water either tap water, rain water or river water with the sewage. (2)
The results of 57 isolates the resistance to more than one antibiotics was common amongst the clinical isolates, there are reports of multi drug resistant vibrio cholera appearing with increasing frequency.
The gentamicin was sensitive to all the isolated strains, and all strains are resistant to Nalidixic acid and Furazolidine.
CONCLUSION: In conclusion there is a need for continued, vigilance and planning effective strategies to provide safe drinking water and simultaneously strengthening the disease surveillance to contain the outbreak of cholera especially in the vulnerable group. And this study helps to prevent the morbidity of the people and high expenditure of the Govt. Fund which was utilizing in the management of the disease.
ACKNOWLEDGEMENT: The author acknowledge the help provided by Dr. B. L. Sarkar, Scientist, National Institute of Cholera and Enteric Disease Kolkata for phage typing and confirmation of vibrios isolates.
(1.) Neelam Kaistha, Manjula Mehta, Vikas Gautam, Varsha Gupta. Outbreak of Cholera in and around Chandigarh during two successive year (2002, 2003). Indian J med (res 122, November 2005 PP404-407).
(2.) R D Kulkarni, S A Patil, V A Kulkarni, M Ramteerthakar, V L Kumbhar, S R Shah, P A Joshi, V L Jahagirdhar. An outbreak of cholera in the Sangli district of Maharashtra. Indian Journal of Medical Microbiology Vol. 25 No: 1 Jan-March 2007: PP76-78.
(3.) Hausler WJ Jr, Sussman M, Tauxe R.V. Cholera, In: Collier L, Balow A, Sussman M Editors, volume authors, Topley and Wilson's Microbiology and Microbial infections 9th ed. Vol. 3 Arnold Publishers: London: 1998 P48-512.
Pramod S. Manthalkar , S. L. Hiremath , Parmeshwarappa K. D 
[1.] Pramod S. Manthalkar
[2.] S. L. Hiremath
[3.] Parmeshwarappa K. D.
PARTICULARS OF CONTRIBUTORS:
[1.] Assistant Professor, Department of Microbiology, BRIMS, Bidar.
[2.] Professor and HOD, Department of Microbiology, BRIMS, Bidar.
[3.] Assistant Professor, Department of Microbiology, BRIMS, Bidar.
NAME ADDRESS EMAIL ID OF THE CORRESPONDING AUTHOR:
Dr. S. L. Hiremath, Professor and HOD, Department of Microbiology, Bidar Institute of Medical Sciences, Bidar-585401, Karnataka.
Date of Submission: 05/11/2014.
Date of Peer Review: 06/11/2014.
Date of Acceptance: 14/11/2014.
Date of Publishing: 19/11/2014.
|Printer friendly Cite/link Email Feedback|
|Title Annotation:||ORIGINAL ARTICLE|
|Author:||Manthalkar, Pramod S.; Hiremath, S.L.; Parmeshwarappa K.D.|
|Publication:||Journal of Evolution of Medical and Dental Sciences|
|Date:||Nov 20, 2014|
|Previous Article:||Spontaneous septostomy in twin pregnancy: a rare and dangerous complication.|
|Next Article:||Asymptomatic extensive pelvic lipomatosis associated with symptomatic epigastric hernia: a rare case report.|