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Chemotaxis; methods and protocols.

9781607611974

Chemotaxis; methods and protocols.

Ed. by Tian Jin and Dale Hereld.

Humana Press Inc.

2009

537 pages

$110.00

Hardcover

RM260

Jin (Laboratory of Immunogenetics, National Institute of Allergy and Infectious Disease, National Institute of Health) and Herald (National Institute on Alcohol, Abuse and Alcoholism of the National Institute of Health) present 33 papers describing state-of-the-art methods for investigating chemotaxis, the directed movement of cells in response to shallow gradients of specific chemical signals in their environments. The methods include experimental procedures for visualizing and measuring migration behaviors of different kinds of organisms; methods for studying signal transduction pathways involved in chemotaxis in D. discoideum; methods for measuring spatiotemporal dynamics of chemoattractant concentrations, activation of receptors, heterotrimeric G-proteins, small G-protein Ras signaling, and actin cytoskeleton assembly using different imaging techniques; and the use of computer-based quantitative models for addressing the complexity of a signaling network. Each chapter discusses the principles underlying the method, lists necessary materials and reagents, describes step-by-step protocols, and includes notes on troubleshooting and pitfalls.

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Publication:SciTech Book News
Article Type:Book review
Date:Jun 1, 2010
Words:176
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