Case report of new rictularia species from stray dog in Iran.
Rectularia (spirorid, Rectularidae) is a common parasite of rodents and carnivores in many parts of the world [1,2,3,4]. Many species of genus Rectularia have already been described but the genus is not common parasite from Iran. In Study on rodent, jackal and dog gastrointestinal parasites, only three species of Rictularia were observed in Iran [5,6,7].
MATERIALS AND METHODS
On April 2013, during the survey of intestinal parasite of stray dogs (Iran, Lorestan province) one mature, male nematode of the genus Rictularia was obtained.
The nematode was fixed in 10% formalin. Then transfered to lactophenol plus azocarmin for clearing and staining. Drawing was made by camera Lucida.
The worm has been armed on cuticular lateral sides with two sub ventral series of large, flattened, comb-like spines. The mouth sub terminally situated, strongly was chitinous and armed. The posterior end is conical and overly curved. Four pairs of papilla (three pairs in pre-anal and one pair in post-anal) and one pair of spicule are seemed.
Descriptions: (all measurement are in mm).
Length 6.7; maximum diameter 0.282 in near posterior end. Mouth sub terminal, without lips and with project out two teeth in floor and denticules in border (6-8) (fig.1d). Oesophagus length 1.878 (0.313 in length and 0.55 in wide in anterior narrower part and 1.565 in length and 0.101 in wide posterior thick part) (fig. 2a and 2b). Nerve ring at 0.159 from anterior end. 218 combs were observe in two rows (109 on each side); beginning 0.035 from anterior end and extending to near anus (0.44 to posterior end) (fig. 1c and 2c). The largest comb was observed at middle of the body with length 0.103. Two or three of posterior end combs are different in shape. Two pair of equal spiculs, 0.275 in length, was observed (fig.3b). In the posterior part of the body, anterior of the cloaca, a row of 8 fans was observed as the following, 1th 0.122, 2th 0.121, 3th 0.127, 4th 0.122, 5th 0.108, 6th 0.108, 7th 0.108 and 8th 0.139 length interior to posterior. These fans are fluted (fig.3a). Three pairs of papilla in pre-anal and one pair post- anal was observed (fig. 4).
RESULTS AND DISCUSSION
Rictularia is uncommon parasite of dogs in Iran. Three species of Rictularia have already been determined in Iran, Rictularia ratti, obtained from rat in south , Rictularia affinis, from stray dog in central  and R. cahirensis from jackls in north of Iran .
In this study one mature male Rictularia was obtained from stray dog in the west of Iran. According to main characters such as the total number of combs(spin), length of spiculs and the number of pre-anal fans, it is approximately resemble to three species of cahorensis, affinis (jagerskiold 1909) and splindida (hall 1911) . Table 1 compares three species of cahorensis, affinis and splindida with author's specimen.
Some scientists like Hall (1913) believed that the number of combs is an important character to differentiation of these species  but others like Kobulj (1951) were not . According to the table 1 the number of combs of author specimen is similar to splindida, close to affinis and noticeable higher than cahirensis. On the other hand, the last comb in affinis is located about 0.5-1.1 mm. anterior to the anus  but in other is near to it (0.05 to 0.1mm.). Because of the low number of specimen, total length of author's specimen is not proper criteria to compare, however it is between to affinis and splindida. Length of author's specimen spicule is larger than others. In the new case the number of fans is just similar to splindida but the length of last fan is larger than it  (110p against to 149p). Auther's specimen as well as splindida possessed three pairs of large conoidal pre-anal and one post-anal papillae but in splindida post-anal pair is near the tip of the tail but in this case near the anus.
Finally there is no significant data to determine the exact location of this specimen. It is identically not comparable to other species. Thus we named its Rictularia canicula.
Rictularia sp Figures. All figures drawn with aid of camera lucida.
1, 2--Anterior End
a--Narrow Portion of Oesophagus
b--Thick Portion of Oesophagus
Received 25 June 2014
Received in revised form 8 July 2014
Accepted 10 August May 2014
Available online 30 August 2014
The current study was supported by Broujerd Branch, Islamic Azad University, Broujerd, Iran. We are grateful of all staff of Broujerd Branch, Islamic Azad University who helped us in this study.
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(1) Farzad Parsa, (2) Hossein Vosoghi, (3) Irej Mobedi, (4) Reza Godarzi, (5) Nilufar Yaritaleb
(1,5) Deparment of Laboratory Sciences, Faculty of Para-Medicine, Broujerd Branch, Islamic Azad University, Broujerd, Iran.
(2,4) Department of Laboratory Sciences, Faculty of veterinary medicine, Broujerd Branch, Islamic Azad University, Broujerd, Iran.
(3) Department of Medical Parasitology and Mycology, School of Public Health and Institute of Public Health Research, Tehran University of Medical Sciences, Tehran, Iran.
Corresponding Author: Farzad Parsa, Department of Laboratory Sciences, Faculty of Para-Medicine, Broujerd Branch, Islamic Azad University, Broujerd, Iran
Table 1: The comparison of the male characteristics of R.affinis, R.cahirensis and R.splindida with author's specimen. Total no. of species pairs of combs Total length(mm.) affinis 111 7-8.5 cahirensis 96 4.8 splindida 108-109 4.83 author's 109 6.8 specimen N0. Precloca or species Spicule length(p) midventral fans affinis 220-230 6 cahirensis 170 7 splindida 207 8 author's 275 8 specimen
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|Author:||Parsa, Farzad; Vosoghi, Hossein; Mobedi, Irej; Godarzi, Reza; Yaritaleb, Nilufar|
|Publication:||Advances in Environmental Biology|
|Article Type:||Clinical report|
|Date:||Jun 1, 2014|
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