CIRCADIAN CLOCK-REGULATED DNA REPAIR GENE XPA IS DOWNREGULATED IN METASTATIC OF LUNG CANCER.
Lung cancer is rarely detected in the early stages of the disease and 60-70% of patients have advanced or metastatic disease at diagnosis. Metastatic spread to other organs is the main cause of death in lung cancer. However, the underlying mechanisms of metastasis remain unknown and therefore further systematic studies are necessary on the possible differences in expression of markers in primary and metastatic tissues. The DNA repair capacity, which is directly involved in tumorigenesis, is controlled by circadian clock through XPA oscillation. Morever, overexpression of DNA repair genes has been suggested to be associated with metastasis. Therefore, we examined the expression of XPA at different stages of carcinogenesis from primary tumors to metastasis in patients with lung cancer.
The purpose of this study was to compare the expression of XPA in the primary tumors and metastatic lymph nodes of 21 lung cancer patients. We have analyzed the expression of XPA in 21 formalin-fixed paraffin-embedded (FFPE) specimens derived from primary non-small cell lung cancer tumors and lymph node metastasis as well as normal lung tissue. FFPE specimens were used to isolate RNA from samples, which were classified as normal lung tissue, primary tumors, and lymph-node metastases according to pathological confirmation.
Our results indicate that XPA had significantly different expression metastatic lymph nodes rather than primary tumors of patients with lung cancer. Against the general expectation that XPA levels would increase in metastatic samples, XPA levels are significantly downregulated in metastatic lymph nodes as compared to normal lung tissue
Targeted chemotherapy of lung cancer is currently based on sensitivity of the primary tumor to specific drugs. These results provide an initial step toward the improvement of lung cancer therapy that is based on measurement of the expression of genes in the metastatic lymph nodes.
Secil Yilmaz (1), Esra Atar (1), Ozlem Canoz (2), Mete Gundog (3), Yusuz Ozkul (1), Gulay Sezer (1), Nuri Ozturk (5)
(1) Genome and Stem Cell Center, Erciyes University, Kayseri, Turkey
(2) Department of Pathology, Erciyes University Faculty of Medicine, Kayseri, Turkey
(3) Department of Radiation Oncology, Erciyes University Faculty of Medicine, Kayseri, Turkey
(4) Molecular Biology and Genetics, Gebze Institute of Technology, Kocaeli, Turkey
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|Title Annotation:||Oral Posters|
|Author:||Yilmaz, Secil; Atar, Esra; Canoz, Ozlem; Gundog, Mete; Ozkul, Yusuz; Sezer, Gulay; Ozturk, Nuri|
|Publication:||Erciyes Medical Journal|
|Date:||Jun 1, 2017|
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